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https://www.readbyqxmd.com/read/29117430/biological-false-positive-venereal-disease-research-laboratory-test-in-cerebrospinal-fluid-in-the-diagnosis-of-neurosyphilis-a-case-control-study
#1
S Zheng, R J Lin, Y H Chan, Ccl Ngan
BACKGROUND: There is no clear consensus on the diagnosis of neurosyphilis. The Venereal Disease Research Laboratory (VDRL) test from cerebrospinal fluid (CSF) has traditionally been considered the gold standard for diagnosing neurosyphilis, but is widely known to be insensitive. OBJECTIVE: In this study, we compared the clinical and laboratory characteristics of true positive VDRL-CSF cases with biological false positive VDRL-CSF cases. METHODS: We retrospectively identified cases of true and false positive VDRL-CSF across a 3-year period received by the Immunology and Serology Laboratory, Singapore General Hospital...
November 8, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29050149/-accuracy-analysis-and-comparison-of-different-serological-detection-methods-in-syphilis
#2
M Hu, N Liu, Y Y Cai, T Lei, M Zhang
Objective: To compare the accuracy of different methods by reviewing syphilis serologic screening external quality assessment (EQA) data of Chinese primary health care institutions. Methods: The EQA was based on one or two distinct shipments from 2011 to 2016. Each shipment consisted of five serum samples. The laboratories performed the EQA and sent back the results by the deadline. The results were analyzed using the standard procedures and methods. Results: Six different methods were used for syphilis serologic screening: enzyme linked immunosorbent assay, colloidal gold method, Treponema pallidum particle agglutination (TPPA) test, rapid plasma reagin card test, toluidine red unheated serum reagin test and chemiluminescence immunoassay...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28855625/serological-response-predicts-normalization-of-cerebrospinal-fluid-abnormalities-at-six-months-after-treatment-in-hiv-negative-neurosyphilis-patients
#3
Yao Xiao, Man-Li Tong, Li-Rong Lin, Li-Li Liu, Kun Gao, Mei-Jun Chen, Hui-Lin Zhang, Wei-Hong Zheng, Shu-Lian Li, Hui-Ling Lin, Zhi-Feng Lin, Tian-Ci Yang, Jian-Jun Niu
This study aimed to determine whether a serological response could predict the normalization of cerebrospinal fluid (CSF) abnormalities at 6 months after treatment in human immunodeficiency virus (HIV)-negative neurosyphilis patients. A total of 123 neurosyphilis patients were recruited at baseline, 58 of these patients undergoing treatment, repeated CSF examinations and serological tests for syphilis at 6 months after treatment were included in the follow-up study. Before treatment, the CSF rapid plasma reagin (RPR) titer, CSF Treponema pallidum particle agglutination (TPPA) titer, CSF leukocyte count, and CSF protein concentration were correlated with both serum RPR and TPPA titers...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28768925/reduction-sensitive-poly-ethylenimine-nanogel-bearing-dithiodipropionic-acid
#4
Huangying Guo, Jin-Chul Kim
Reduction-sensitive nanogel was developed by including dithiodipropionic acid (DTPA) in tripolyphosphoric acid (TPPA) cross-linked poly(ethylenimine) (PEI) nanogel. According to the light scattering measurement, DTPA (a disulfide compound) seemed to cross-link PEI chains in a cooperative manner with TPPA (a multi-valent anion). Nanogels composed of TPPA, PEI, and DTPA exhibited negative zeta potential and the absolute value increased with the amount of TPPA and DTPA. TPPA and DTPA were found to be contained in the nanogel, evidenced by Fourier transform (FT)-IR spectroscopy and Raman spectroscopy, respectively...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28765847/highly-efficient-enrichment-of-n-linked-glycopeptides-using-a-hydrophilic-covalent-organic-framework
#5
Yu-Fang Ma, Fang Yuan, Xiao-Hui Zhang, Ying-Lin Zhou, Xin-Xiang Zhang
The enrichment of glycopeptides plays an important role in glycoproteomics. In this paper, a covalent-organic framework called TpPa-1, synthesized by the Schiff base reaction of 1,3,5-triformylphloroglucinol and paraphenylenediamine, was first successfully utilized as a hydrophilic porous material for N-linked glycopeptide enrichment. Using this material, interference from non-glycopeptides could be efficiently eliminated, which facilitated the mass spectrometry detection of glycopeptides. By capturing N-linked glycopeptides from tryptic digests of human IgG, our method was proved to have high sensitivity at the femtomole level...
August 2, 2017: Analyst
https://www.readbyqxmd.com/read/28727773/the-tradition-algorithm-approach-underestimates-the-prevalence-of-serodiagnosis-of-syphilis-in-hiv-infected-individuals
#6
COMPARATIVE STUDY
Bin Chen, Xiuming Peng, Tiansheng Xie, Changzhong Jin, Fumin Liu, Nanping Wu
Currently, there are three algorithms for screening of syphilis: traditional algorithm, reverse algorithm and European Centre for Disease Prevention and Control (ECDC) algorithm. To date, there is not a generally recognized diagnostic algorithm. When syphilis meets HIV, the situation is even more complex. To evaluate their screening performance and impact on the seroprevalence of syphilis in HIV-infected individuals, we conducted a cross-sectional study included 865 serum samples from HIV-infected patients in a tertiary hospital...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28661234/an-evaluation-of-the-sd-bioline-hiv-syphilis-duo-test
#7
Jeffrey Holden, Joshua Goheen, Mary Jett-Goheen, Mathilda Barnes, Yu-Hsiang Hsieh, Charlotte A Gaydos
Many health agencies now recommend routine HIV and syphilis testing for pregnant women and most-at-risk populations such as men who have sex with men. With the increased availability of highly sensitive, low cost rapid point-of-care tests, the ability to meet those recommendations has increased, granting wider access to quick and accurate diagnoses. Using blood specimens collected from a Baltimore City Health Department (BCHD) sexually transmitted infection clinic, we evaluated the SD Bioline HIV/Syphilis Duo, a rapid test that simultaneously detects antibodies to HIV and syphilis and has the potential to further benefit clinics and patients by reducing costs, testing complexity, and patient wait times...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28660379/hiv-incidence-and-care-linkage-among-msm-first-time-testers-in-shenyang-china-2012-2014
#8
Jing Zhang, Jun-Jie Xu, Wei Song, Shi Pan, Zhen-Xing Chu, Qing-Hai Hu, Huan Yu, Xiang Mao, Yong-Jun Jiang, Wen-Qing Geng, Hong Shang, Ning Wang
HIV testing is the first step to the fulfillment of Treat as Prevention (TasP) and reaching the 90-90-90 goal in HIV control. However, there are still a large number of Men who have Sex with Men (MSM) have never been tested for HIV before, and little is known about the HIV incidence and care linkage among this population. A Mixed method was used to recruit MSM who had never tested for HIV before from January 2012 to December 2014 in Shenyang, China. Potential MSM participants were invited to attend the enrollment for HIV and syphilis testing at a general hospital-based voluntary counseling and test (VCT)...
June 30, 2017: AIDS and Behavior
https://www.readbyqxmd.com/read/28614239/the-analyzation-and-clinical-evaluation-of-eclia-and-cmia-in-the-detection-of-treponema-pallidum
#9
COMPARATIVE STUDY
Jiansuo Zhou, Yongming Liang, Jie Zhang, Liyan Cui
To evaluate the clinical application value of electrochemiluminescence immunoassay analyzer (ECLIA) and chemiluminescent magnetic microparticle immunoassay (CMIA) in the detection of Treponema pallidum (TP).A total of 1225 patients in Peking University Third Hospital was enrolled from June 2014 to October 2014. ECLIA and CMIA were applied to detect the serum anti-TP. The positive rate was analyzed. RIBA was taken as a golden standard to evaluate the sensitivity, the specificity, the positive predictive value, the negative predictive value, and the accuracy of ECLIA and CMIA...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28446129/novel-predictors-of-neurosyphilis-among-hiv-negative-syphilis-patients-with-neurological-symptoms-an-observational-study
#10
Yao Xiao, Man-Li Tong, Li-Li Liu, Li-Rong Lin, Mei-Jun Chen, Hui-Lin Zhang, Wei-Hong Zheng, Shu-Lian Li, Hui-Ling Lin, Zhi-Feng Lin, Hui-Qin Xing, Jian-Jun Niu, Tian-Ci Yang
BACKGROUND: Known predictors of neurosyphilis were mainly drawn from human immunodeficiency virus (HIV)-infected syphilis patients, which may not be applicable to HIV-negative populations as they have different characteristics, particularly those with neurological symptoms. This study aimed to identify novel predictors of HIV-negative symptomatic neurosyphilis (S-NS). METHODS: From June 2005 to June 2015, 370 HIV-negative syphilis patients with neurological symptoms were recruited, consisting of 191 S-NS patients (including 123 confirmed neurosyphilis and 68 probable neurosyphilis patients) and 179 syphilis/non-neurosyphilis (N-NS) patients...
April 26, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28400821/the-great-impostor-transaminitis-masking-the-coinfection-of-syphilis-and-human-immunodeficiency-virus
#11
Sunit Tolia, Hassan Kassem, Ana Capatina-Rata
Introduction. The incidence of syphilis continues to rise in the United States over the past 15 years. This disease process is classified into stages and may present with a coinfection of Human Immunodeficiency Virus (HIV). Case Report. We present a case of a 32-year-old African American male who presented with cutaneous manifestations of secondary syphilis and transaminitis. A workup revealed that the transaminitis was secondary to underlying syphilitic hepatitis in the presence of HIV coinfection. The patient had a reactive rapid plasma reagin (RPR) of 1 : 64 TU and reactive Treponema pallidum particle agglutination assay (TPPA)...
2017: Case Reports in Medicine
https://www.readbyqxmd.com/read/28381602/cerebrospinal-fluid-treponema-pallidum-particle-agglutination-assay-for-neurosyphilis-diagnosis
#12
Christina M Marra, Clare L Maxwell, Shelia B Dunaway, Sharon K Sahi, Lauren C Tantalo
Limited data suggest that the cerebrospinal fluid Treponema pallidum particle agglutination assay (CSF-TPPA) is sensitive and a CSF Treponema pallidum hemagglutination assay (CSF-TPHA) titer of ≥1:640 is specific for neurosyphilis diagnosis. CSF-TPPA reactivity and titer were determined for a convenience sample of 191 CSF samples from individuals enrolled in a study of CSF abnormalities in syphilis (training data set). The sensitivity of a reactive test and the specificity for reactivity at serial higher CSF dilutions were determined...
June 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28256292/evaluation-of-the-automated-advia-centaur%C3%A2-xp-syphilis-assay-for-serological-testing
#13
Sharon Saw, Huiqin Zhao, Phyllis Tan, Betty Saw, Sunil Sethi
We evaluated the performance of the ADVIA Centaur XP Syphilis assay (Siemens Healthcare Diagnostics, Tarrytown, NY, USA) using samples previously tested on the ARCHITECT i4000SR system (Abbott Diagnostics, Lake Forest, IL, USA) and confirmed by the Treponema pallidum particle agglutination assay (TPPA) (SERODIA-TPPA, Fujirebio Diagnostics Inc., Malvern, PA, USA). Clinical patient information was included to aid resolution of discordant samples where available. Precision, interference, and cross-reactivity were also assessed...
May 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28213213/titanium-iv-ion-modified-covalent-organic-frameworks-for-specific-enrichment-of-phosphopeptides
#14
Heping Wang, Fenglong Jiao, Fangyuan Gao, Yayao Lv, Qiong Wu, Yan Zhao, Yehua Shen, Yangjun Zhang, Xiaohong Qian
To date, plenty of new alternative materials for phosphopeptides enrichment prior to mass spectrometry (MS) analysis appear, especially immobilized metal ion affinity chromatography (IMAC) materials. The variable combinations with different metal ions, chelating ligands and solid supports offer full of optionality for IMAC. However, further improvement was predicted by the tedious and complex synthetic process. In this work, a novel covalent organic framework (COF)-based IMAC material (denoted TpPa-2-Ti(4+)) was prepared simply by direct immobilizing Ti (IV) into TpPa-2 COFs without any extra chelating ligands...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28196838/screening-for-asymptomatic-neurosyphilis-in-hiv-patients-after-treatment-of-early-syphilis-an-observational-study
#15
Andrew Tomkins, Shazaad Ahmad, Darren E Cousins, Caroline M Thng, Francisco Javier Vilar, Stephen P Higgins
OBJECTIVE: To determine the prevalence of asymptomatic neurosyphilis (ANS) in HIV-positive individuals after treatment of early syphilis with single-dose benzathine penicillin G (BPG) or oral antibiotic alternatives. METHODS: Patients at high risk of neurosyphilis (defined by serum rapid plasma reagin (RPR) titre ≥1:32 and/or peripheral blood CD4 lymphocyte count ≤350/μL) underwent lumbar puncture (LP) at a median time of 8.2 months post treatment. ANS was diagnosed by a reactive cerebrospinal fluid (CSF) RPR test or CSF white blood cells (WBC) >20/μL plus a reactive CSF Treponema pallidum particle agglutination (TPPA) ≥1:640...
February 14, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28078417/covalent-organic-framework-coated-magnetic-graphene-as-a-novel-support-for-trypsin-immobilization
#16
Heping Wang, Fenglong Jiao, Fangyuan Gao, Xinyuan Zhao, Yan Zhao, Yehua Shen, Yangjun Zhang, Xiaohong Qian
Deep and efficient proteolysis is the critical premise in mass spectrometry-based bottom-up proteomics. It is difficult for traditional in-solution digestion to meet the requirement unless prolonged digestion time and enhanced enzyme dosage are employed, which makes the whole workflow time-consuming and costly. The abovementioned problems could be effectively ameliorated by anchoring many proteases on solid supports. In this work, covalent organic framework-coated magnetic graphene (MG@TpPa-1) was designed and prepared as a novel enzyme carrier for the covalent immobilization of trypsin with a high degree of loading (up to 268 μg mg(-1))...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28075557/facile-synthesis-of-magnetic-covalent-organic-framework-with-three-dimensional-bouquet-like-structure-for-enhanced-extraction-of-organic-targets
#17
Sijing He, Tao Zeng, Saihua Wang, Hongyun Niu, Yaqi Cai
A facile strategy for the fabrication of novel bouquet-shaped magnetic porous nanocomposite via grafting a covalent organic framework (COF, TpPa-1) onto the surface-modified Fe3O4 nanoparticles (Fe3O4 NPs) was reported. The magnetic TpPa-1 (a COF synthesized from 1,3,5-triformylphloroglucinol (Tp) and p-phenylenediamine (Pa-1)) contains clusters of core-shell magnetic nanoparticles and interconnected porous TpPa-1 nanofibers. Thus, it possesses larger specific surface area, higher porosity, and supermagnetism, making it an ideal sorbent for enrichment of trace analytes...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28009865/two-blue-light-excitable-yellow-emitting-lmof-phosphors-constructed-by-triangular-tri-4-pyridylphenyl-amine
#18
Fangming Wang, Zeyu Zhou, Wei Liu, Lei Zhou, Lizhuang Chen, Jing Li
Two luminescent metal-organic-frameworks (LMOFs) constructed by a molecular chromophore [tri(4-pyridylphenyl)amine] (tppa) were synthesized by solvothermal reactions and characterized by single crystal and powder X-ray diffraction, thermogravimetric analysis, optical diffuse reflection, photoluminescence spectroscopy, and internal quantum yield measurements. Both compound 1 [Zn(tppa)(ndc)·(DMF)4] and compound 2 [Zn4(tppa)2(sdc)3(NO3)2·(DMF)4·(ACN)2] (ndc = 2,6-naphthalenedicarboxylic acid, sdc = (E)-4,4'-(ethene-1,2-diyl)dibenzoic acid, DMF = dimethylformamide, ACN = acetonitrile) exhibit intense yellow emission under blue light (455 nm) excitation...
December 23, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27998453/-clinical-features-of-neurosyphilis-with-optic-neuritis-as-an-initial-finding
#19
S S Cao, H Y Li, Q G Xu, S Y Tan, S H Wei
Objective: To study the characteristics of neurosyphilis with optic neuritis as an initial finding. Methods: Retrospective analysis of clinical data and laboratory testing results of 16 cases (27 eyes) with optic neuritis as an initial finding of neurosyphilis from October 2010 to March 2015 in General Hospital of People's Liberation Army was made. Results: Six-teen patients (12 males, 4 females) were collected, the median age of patients was 47 (range 33 to 65) years ,the mean age was (49.63±9.05) years. Treponema pallidum particle agglutination assay (TPPA) analysis was positive in all of the patients and rapid plasma reagin (RPR) test was positive in 14 patients (2 patients did not test)...
December 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27852877/community-seroprevalence-survey-for-yaws-and-trachoma-in-the-western-division-of-fiji
#20
Naomi Cocks, Merelesita Rainima-Qaniuci, Chelsea Yalen, Colin Macleod, Apisalome Nakolinivalu, Stephanie Migchelsen, Chrissy H Roberts, Robert Butcher, Mike Kama, David Mabey, Michael Marks
BACKGROUND: Both yaws and trachoma are endemic in several countries in the Pacific. In co-endemic countries there may be potential synergies between both control programmes. METHODS: We undertook a cluster randomised trachoma and yaws seroprevalence survey of children in the Western Division of Fiji. Children were examined for skin lesions consistent with active yaws. A dried blood spot was collected which was tested using the Treponema pallidum particle agglutination (TPPA) test and an ELISA to detect antibodies against Pgp3...
December 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
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