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https://www.readbyqxmd.com/read/28078417/covalent-organic-framework-coated-magnetic-graphene-as-a-novel-support-for-trypsin-immobilization
#1
Heping Wang, Fenglong Jiao, Fangyuan Gao, Xinyuan Zhao, Yan Zhao, Yehua Shen, Yangjun Zhang, Xiaohong Qian
Deep and efficient proteolysis is the critical premise in mass spectrometry-based bottom-up proteomics. It is difficult for traditional in-solution digestion to meet the requirement unless prolonged digestion time and enhanced enzyme dosage are employed, which makes the whole workflow time-consuming and costly. The abovementioned problems could be effectively ameliorated by anchoring many proteases on solid supports. In this work, covalent organic framework-coated magnetic graphene (MG@TpPa-1) was designed and prepared as a novel enzyme carrier for the covalent immobilization of trypsin with a high degree of loading (up to 268 μg mg(-1))...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28075557/facile-synthesis-of-magnetic-covalent-organic-framework-with-three-dimensional-bouquet-like-structure-for-enhanced-extraction-of-organic-targets
#2
Sijing He, Tao Zeng, Saihua Wang, Hongyun Niu, Yaqi Cai
A facile strategy for the fabrication of novel bouquet-shaped magnetic porous nanocomposite via grafting a covalent organic framework (COF, TpPa-1) onto the surface-modified Fe3O4 nanoparticles (Fe3O4 NPs) was reported. The magnetic TpPa-1 (a COF synthesized from 1,3,5-triformylphloroglucinol (Tp) and p-phenylenediamine (Pa-1)) contains clusters of core-shell magnetic nanoparticles and interconnected porous TpPa-1 nanofibers. Thus, it possesses larger specific surface area, higher porosity, and supermagnetism, making it an ideal sorbent for enrichment of trace analytes...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28009865/two-blue-light-excitable-yellow-emitting-lmof-phosphors-constructed-by-triangular-tri-4-pyridylphenyl-amine
#3
Fangming Wang, Zeyu Zhou, Wei Liu, Lei Zhou, Lizhuang Chen, Jing Li
Two luminescent metal-organic-frameworks (LMOFs) constructed by a molecular chromophore [tri(4-pyridylphenyl)amine] (tppa) were synthesized by solvothermal reactions and characterized by single crystal and powder X-ray diffraction, thermogravimetric analysis, optical diffuse reflection, photoluminescence spectroscopy, and internal quantum yield measurements. Both compound 1 [Zn(tppa)(ndc)·(DMF)4] and compound 2 [Zn4(tppa)2(sdc)3(NO3)2·(DMF)4·(ACN)2] (ndc = 2,6-naphthalenedicarboxylic acid, sdc = (E)-4,4'-(ethene-1,2-diyl)dibenzoic acid, DMF = dimethylformamide, ACN = acetonitrile) exhibit intense yellow emission under blue light (455 nm) excitation...
December 23, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27998453/-clinical-features-of-neurosyphilis-with-optic-neuritis-as-an-initial-finding
#4
S S Cao, H Y Li, Q G Xu, S Y Tan, S H Wei
Objective: To study the characteristics of neurosyphilis with optic neuritis as an initial finding. Methods: Retrospective analysis of clinical data and laboratory testing results of 16 cases (27 eyes) with optic neuritis as an initial finding of neurosyphilis from October 2010 to March 2015 in General Hospital of People's Liberation Army was made. Results: Six-teen patients (12 males, 4 females) were collected, the median age of patients was 47 (range 33 to 65) years ,the mean age was (49.63±9.05) years. Treponema pallidum particle agglutination assay (TPPA) analysis was positive in all of the patients and rapid plasma reagin (RPR) test was positive in 14 patients (2 patients did not test)...
December 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27852877/community-seroprevalence-survey-for-yaws-and-trachoma-in-the-western-division-of-fiji
#5
Naomi Cocks, Merelesita Rainima-Qaniuci, Chelsea Yalen, Colin Macleod, Apisalome Nakolinivalu, Stephanie Migchelsen, Chrissy H Roberts, Robert Butcher, Mike Kama, David Mabey, Michael Marks
BACKGROUND: Both yaws and trachoma are endemic in several countries in the Pacific. In co-endemic countries there may be potential synergies between both control programmes. METHODS: We undertook a cluster randomised trachoma and yaws seroprevalence survey of children in the Western Division of Fiji. Children were examined for skin lesions consistent with active yaws. A dried blood spot was collected which was tested using the Treponema pallidum particle agglutination (TPPA) test and an ELISA to detect antibodies against Pgp3...
December 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27835625/use-of-treponemal-screening-assay-strength-of-signal-to-avoid-unnecessary-confirmatory-testing
#6
Gregory J Berry, Michael J Loeffelholz
BACKGROUND: Our reverse syphilis testing algorithm consists of a treponemal IgG multiplex flow immunoassay (MFI) followed by both rapid plasma reagin titer and the Treponema pallidum particle agglutination (TPPA) test on specimens with a reactive MFI result. We report here the impact of a modified reverse algorithm, in which the strength of signal of the MFI is used to avoid unnecessary TPPA testing. METHODS: The Bioplex syphilis IgG MFI was used as the syphilis screening assay, and specimens with equivocal (antibody index 0...
December 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27800128/the-distribution-of-syphilis-among-inpatients-in-wenzhou-china-a-hospital-based-study
#7
Ke Xu, Shengying Chi, Bin Chen, Lingzhi Chen, Dongyun Zheng
BACKGROUND: The high prevalence of syphilis among inpatients is an important concern in clinical settings. Thus, a better understanding of the serological test would be valuable. OBJECTIVES: We analyzed the serological test results for syphilis among the inpatients in Wenzhou central hospital, China, to estimate the distribution of syphilis this Chinese population. PATIENTS AND METHODS: The blood samples of 81946 inpatients at the hospital from January 2010 to December 2012 were collected and retrospectively analyzed...
August 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27602569/risk-factors-associated-with-incident-syphilis-in-a-cohort-of-high-risk-men-in-peru
#8
Hayoung Park, Kelika A Konda, Chelsea P Roberts, Jorge L Maguiña, Segundo R Leon, Jesse L Clark, Thomas J Coates, Carlos F Caceres, Jeffrey D Klausner
BACKGROUND: Syphilis is concentrated among high-risk groups, but the epidemiology of syphilis reinfection is poorly understood. We characterized factors associated with syphilis incidence, including reinfection, in a high-risk cohort in Peru. METHODS: Participants in the NIMH CPOL trial were assessed at baseline and 2 annual visits with HIV/STI testing and behavioral surveys. Participants diagnosed with syphilis also attended 4- and 9-month visits. All participants underwent syphilis testing with RPR screening and TPPA confirmation...
2016: PloS One
https://www.readbyqxmd.com/read/27551787/prevalence-of-active-and-latent-yaws-in-the-solomon-islands-18-months-after-azithromycin-mass-drug-administration-for-trachoma
#9
Michael Marks, Oliver Sokana, Eli Nachamkin, Elliot Puiahi, Georgina Kilua, Allan Pillay, Christian Bottomley, Anthony W Solomon, David C Mabey
INTRODUCTION: Both yaws and trachoma are endemic in the Pacific. Mass treatment with azithromycin is the mainstay of the WHO strategy for both the eradication of yaws and the elimination of trachoma as a public health problem, but the dose recommended for trachoma is lower than that for yaws. In countries where both diseases are endemic, there is a potential for synergy between yaws and trachoma control programs if mass treatment with the lower dose of azithromycin was shown to be effective for the treatment of yaws...
August 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27346117/-analysis-on-the-clinical-value-of-methods-used-for-the-detection-of-treponema-pallidum-antibody
#10
F Xia, Y H Xu, X L Wang
OBJECTIVE: To explore the clinical value of five methods commonly used for the detection of clinical syphilis antibody. METHODS: A total of 160 confirmed syphilis cases were chosen as the experimental group while 200 non-syphilis cases were set as the control group. Serum specimens were detected by methods as Treponema pallidum particle agglutination assay (TPPA), chemiluminescent microparticle immune assay (CMIA), enzyme linked immunosorbent assay (ELISA), emulsion method (TP-AD) and toluidine red unheated serum test (TRUST)...
June 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/27265351/the-trans-pacific-partnership-agreement-and-health-few-gains-some-losses-many-risks
#11
Ronald Labonté, Ashley Schram, Arne Ruckert
BACKGROUND: In early October 2015, 12 nations signed the Trans-Pacific Partnership Agreement (TPPA), promoted as a model '21(st) century' trade and investment agreement that other countries would eventually join. There are growing concerns amongst the public health community about the potential health implications of such WTO+ trade and investment agreements, but little existing knowledge on their potential health impacts. METHODS AND RESULTS: We conducted a health impact review which allows for a summary estimation of the most significant health impacts of a set of policies, in our case the TPPA...
2016: Globalization and Health
https://www.readbyqxmd.com/read/27249890/performance-of-chemiluminescent-microparticle-immunoassay-in-screening-for-syphilis-in-pregnant-women-from-low-prevalence-resource-limited-setting
#12
Amphiwan Boonchaoy, Piya Wongchampa, Nattiya Hirankarn, Surasith Chaithongwongwatthana
OBJECTIVE: This study evaluated the diagnostic performance of chemiluminescent microparticle immunoassay (CMIA) in screening for syphilis in Asian pregnant women. MATERIAL AND METHOD: This study retrospectively reviewed the CMA results of pregnant women attending the antenatal care clinic, King Chulalongkorn Memorial Hospital. Women with reactive CMA results were extracted from the laboratory database and further analyzed. A reactive/positive result for Treponema pallidum was defined as having a sample/cut-off absorbance ratio of ≥ 1...
February 2016: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
https://www.readbyqxmd.com/read/27183698/tppa-nurse-protestor-unlikety-to-lose-her-job
#13
Susan Hornsby-Geluk
No abstract text is available yet for this article.
March 2016: Nursing New Zealand, Kai Tiaki
https://www.readbyqxmd.com/read/27183678/protest-helps-debate-on-tppa-says-butler
#14
(no author information available yet)
No abstract text is available yet for this article.
March 2016: Nursing New Zealand, Kai Tiaki
https://www.readbyqxmd.com/read/27154184/field-evaluation-of-standard-diagnostics-bioline-hiv-syphilis-duo-test-among-female-sex-workers-in-johannesburg-south-africa
#15
Vivian Black, Brian G Williams, Venessa Maseko, Frans Radebe, Helen V Rees, David A Lewis
BACKGROUND: Point-of-care tests provide immediate results with the opportunity for same-day interventions with improved public health outcomes. A dual HIV/syphilis test enables early treatment of both diseases. METHODS: We conducted a field evaluation of the Standard Diagnostics' SD Bioline HIV/Syphilis Duo test (SD Bioline) among female sex workers. SD Bioline was conducted on finger-prick blood according to manufacturer's instructions and compared with (i) Genscreen HIV1/2 (third generation) and Vironostika Ag/Ab (fourth generation) assays for HIV, and (ii) Treponema pallidum particle agglutination (TPPA) and rapid plasma reagin (RPR) assays for syphilis...
May 6, 2016: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/27109871/barium-borohydride-chlorides-synthesis-crystal-structures-and-thermal-properties
#16
Elisabeth Grube, Cathrine H Olesen, Dorthe B Ravnsbæk, Torben R Jensen
Here we report the synthesis, mechanism of formation, characterization and thermal decomposition of new barium borohydride chlorides prepared by mechanochemistry and thermal treatment of MBH4-BaCl2, M = Li, Na or K in ratios 1 : 1 and 1 : 2. Initially, orthorhombic barium chloride, o-BaCl2 transforms into o-Ba(BH4)xCl2-x, x ∼ 0.15. Excess LiBH4 leads to continued anion substitution and a phase transformation into hexagonal barium borohydride chloride h-Ba(BH4)xCl2-x, which accommodates higher amounts of borohydride, possibly x ∼ 0...
May 10, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27039568/thousands-protest-tppa-signing
#17
(no author information available yet)
No abstract text is available yet for this article.
February 2016: Nursing New Zealand, Kai Tiaki
https://www.readbyqxmd.com/read/26962086/evaluation-of-multiplex-based-antibody-testing-for-use-in-large-scale-surveillance-for-yaws-a-comparative-study
#18
Gretchen M Cooley, Oriol Mitja, Brook Goodhew, Allan Pillay, Patrick J Lammie, Arnold Castro, Penias Moses, Cheng Chen, Tun Ye, Ronald Ballard, Diana L Martin
WHO has targeted yaws for global eradication by 2020. The program goals are to interrupt the transmission in countries where yaws is endemic and to certify countries as yaws free where yaws was endemic in the past. No new rapid plasmin reagin (RPR) seroreactivity in young children is required for certification of elimination at a country level. We sought to evaluate whether antibody responses to specific treponemal antigens measured in a high-throughput multiplex bead array (MBA) assay differentiate past versus current infection and whether a nontreponemal lipoidal antigen test can be incorporated into the MBA...
May 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/26914417/tppa-should-not-be-adopted-without-a-full-independent-health-assessment
#19
EDITORIAL
Gay Keating, Joshua Freeman, Alex Macmillan, Pat Neuwelt, Eric Monasterio
No abstract text is available yet for this article.
February 19, 2016: New Zealand Medical Journal
https://www.readbyqxmd.com/read/26865381/chemically-stable-covalent-organic-framework-cof-polybenzimidazole-hybrid-membranes-enhanced-gas-separation-through-pore-modulation
#20
Bishnu P Biswal, Harshal D Chaudhari, Rahul Banerjee, Ulhas K Kharul
Highly flexible, TpPa-1@PBI-BuI and TpBD@PBI-BuI hybrid membranes based on chemically stable covalent organic frameworks (COFs) could be obtained with the polymer. The loading obtained was substantially higher (50 %) than generally observed with MOFs. These hybrid membranes show an exciting enhancement in permeability (about sevenfold) with appreciable separation factors for CO2/N2 and CO2/CH4. Further, we found that with COF pore modulation, the gas permeability can be systematically enhanced.
March 24, 2016: Chemistry: a European Journal
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