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https://www.readbyqxmd.com/read/28886021/complete-genome-sequences-of-two-strains-of-treponema-pallidum-subsp-pertenue-from-ghana-africa-identical-genome-sequences-in-samples-isolated-more-than-7-years-apart
#1
Michal Strouhal, Lenka Mikalová, Pavla Havlíčková, Paolo Tenti, Darina Čejková, Ivan Rychlík, Sylvia Bruisten, David Šmajs
BACKGROUND: Treponema pallidum subsp. pertenue (TPE) is the causative agent of yaws, a multi-stage disease, endemic in tropical regions of Africa, Asia, Oceania, and South America. To date, four TPE strains have been completely sequenced including three TPE strains of human origin (Samoa D, CDC-2, and Gauthier) and one TPE strain (Fribourg-Blanc) isolated from a baboon. All TPE strains are highly similar to T. pallidum subsp. pallidum (TPA) strains. The mutation rate in syphilis and related treponemes has not been experimentally determined yet...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28878003/evaluation-of-the-lumipulse%C3%A2-g-tp-n-chemiluminescent-immunoassay-as-a-syphilis-screening-test
#2
Daniel A Ortiz, Michael J Loeffelholz
A syphilis diagnosis is often aided by the detection of treponemal and non-treponemal antibodies. Automated treponemal antibody detection systems enable high-volume clinical laboratories to perform syphilis screening at a faster pace with lower labor costs. The Lumipulse G TP-N chemiluminescent immunoassay is an automated system that qualitatively detects IgG and IgM antibodies against Treponema pallidum antigens in human serum and plasma. To assess performance characteristics and workflow efficiency, the Lumipulse G TP-N assay was compared to the Bioplex 2200 Syphilis IgG multiplex flow immunoassay...
September 6, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28876300/what-is-the-role-of-paired-rapid-plasma-reagin-testing-simultaneous-testing-of-acute-and-convalescent-samples-in-the-diagnosis-of-repeat-syphilis-and-the-follow-up-of-syphilis
#3
Chris R Kenyon, Kara Krista Osbak, Marjan Van Esbroek, Lutgarde Lynen, Tania Crucitti
BACKGROUND: Repeat syphilis is playing an increasing role in syphilis transmission in several populations. The assessment of repeat syphilis and response to treatment depends on accurately measuring intraindividual changes in non-treponemal tests. For a 0- to 6-month delta rapid plasma reagin (RPR) to be determined by routine individual RPR testing, samples are tested 6 months apart with differences in reagent batches, environmental conditions, and observers all leading to measurement errors...
July 20, 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28856228/traditional-versus-reverse-syphilis-algorithms-a-comparison-at-a-large-academic-medical-center
#4
Craig D Dunseth, Bradley A Ford, Matthew D Krasowski
OBJECTIVES: An increasing number of institutions are transitioning from the traditional syphilis testing algorithm (initial screening with nontreponemal tests) to the 'reverse' algorithm (initial screening with treponemal tests such as syphilis IgG). The aim of this study was to evaluate the switch in syphilis algorithm at an academic medical center with a population with low syphilis prevalence. DESIGN AND METHODS: We performed a six-year retrospective study at the University of Iowa Hospitals and Clinics, an academic medical center, comparing the traditional algorithm (n=12,612) with the reverse algorithm (n=10,453)...
August 2017: Pract Lab Med
https://www.readbyqxmd.com/read/28844341/comparing-three-treponemal-tests-for-syphilis-screening
#5
Sean A Buono, Hilary A Godwin, Nicole M Green
We compared the performance and ease of use for three high-throughput treponemal immunoassays: Phoenix Biotech Trep-Sure Total Antibody EIA, Siemens ADVIA® Centaur Syphilis Assay, and DiaSorin LIAISON® Treponema Assay. One thousand serum samples submitted for routine screening were used in this study. Each assay demonstrated comparable sensitivity, specificity, and percent agreement (98-100%) compared with Treponema pallidum particle agglutination (TP-PA). Thus, treponemal immunoassays are an acceptable alternative for syphilis screening or confirmatory testing...
July 26, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28840989/comparison-of-traditional-and-reverse-syphilis-screening-algorithms-in-medical-health-checkups
#6
Eun Hee Nah, Seon Cho, Suyoung Kim, Han Ik Cho, Jong Yil Chai
BACKGROUND: The syphilis diagnostic algorithms applied in different countries vary significantly depending on the local syphilis epidemiology and other considerations, including the expected workload, the need for automation in the laboratory and budget factors. This study was performed to investigate the efficacy of traditional and reverse syphilis diagnostic algorithms during general health checkups. METHODS: In total, 1,000 blood specimens were obtained from 908 men and 92 women during their regular health checkups...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28820347/association-between-eye-diagnosis-and-positive-syphilis-test-results-in-a-large-urban-sexually-transmitted-infection-primary-care-clinic-population
#7
Ann-Marie Lobo, Yan Gao, Laura Rusie, Magda Houlberg, Supriya D Mehta
In 2015, the Centers for Disease Control and Prevention (CDC) and the American Academy of Ophthalmology (AAO) released clinical advisories on rising cases of ocular syphilis. We examined the association between eye disease and syphilis infection among primary care and sexually transmitted infection (STI) clinic patients attending an urban lesbian, gay, bisexual, transgender (LGBT) health center. We conducted a retrospective medical record review of all patients who underwent syphilis testing at Howard Brown Health between 1 January 2010 and 31 December 2015...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28792374/evaluation-of-an-automated-quantitative-latex-immunoturbidimetric-non-treponemal-assay-for-diagnosis-and-follow-up-of-syphilis-a-prospective-cohort-study
#8
Kara Osbak, Said Abdellati, Achilleas Tsoumanis, Marjan Van Esbroeck, Luc Kestens, Tania Crucitti, Chris Kenyon
PURPOSE: We evaluated the Sekure rapid plasma reagin (RPR-S) (Sekisui Diagnostics) automated quantitative latex immunoturbidimetric assay performed on the SK500 clinical chemistry system for clinical appropriateness. METHODOLOGY: Syphilis-infected individuals and controls were recruited into a prospective cohort study conducted at a sexually transmitted infection clinic in Antwerp, Belgium. Sera collected at diagnosis (baseline) and at 3, 6, 9 and 12 months post-treatment were tested with RPR-S and Macro-Vue RPR card (RPR-C) (Becton Dickinson) assays; RPR-C was considered the reference test...
August 10, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28728181/the-value-of-diagnostic-work-up-in-the-evaluation-of-white-dot-syndromes
#9
Neal V Palejwala, Gagan Sawhney, Matthew Raecker, Chrstina J Flaxel, Steven Yeh
BACKGROUND AND OBJECTIVE: To determine the diagnostic yield of systemic work-up in white dot syndromes. PATIENTS AND METHODS: A retrospective chart review. RESULTS: Eighty-six consecutive patients with a diagnosis of a white dot syndrome were identified. Forty-three had a diagnosis of birdshot chorioretinopathy. Overall, 395 diagnostic tests were performed with a diagnostic yield of 11.9%. The test with the greatest diagnostic yield was HLA-A29 typing (89%)...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28653418/syphilis-testing-practices-in-the-americas
#10
Thuy T Trinh, Mary L Kamb, Minh Luu, D Cal Ham, Freddy Perez
OBJECTIVE: To present the findings of the Pan American Health Organization's 2014 survey on syphilis testing policies and practices in the Americas. METHODS: Representatives of national/regional reference and large, lower-level laboratories from 35 member states were invited to participate. A semi-structured, electronically administered questionnaire collected data on syphilis tests, algorithms, equipment/commodities, challenges faced and basic quality assurance (QA) strategies employed (i...
September 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28642342/survival-of-contagious-ovine-digital-dermatitis-codd-associated-treponemes-on-disposable-gloves-after-handling-codd-affected-feet
#11
J W Angell, S R Clegg, D H Grove-White, R W Blowey, S D Carter, J S Duncan, N J Evans
No abstract text is available yet for this article.
July 22, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28542573/development-of-real-time-pcr-and-loop-mediated-isothermal-amplification-lamp-assays-for-the-differential-detection-of-digital-dermatitis-associated-treponemes
#12
Kelly Anklam, Megan Kulow, Wataru Yamazaki, Dörte Döpfer
Bovine digital dermatitis (DD) is a severe infectious cause of lameness in cattle worldwide, with important economic and welfare consequences. There are three treponeme phylogroups (T. pedis, T. phagedenis, and T. medium) that are implicated in playing an important causative role in DD. This study was conducted to develop real-time PCR and loop-mediated isothermal amplification (LAMP) assays for the detection and differentiation of the three treponeme phylogroups associated with DD. The real-time PCR treponeme phylogroup assays targeted the 16S-23S rDNA intergenic space (ITS) for T...
2017: PloS One
https://www.readbyqxmd.com/read/28499290/molecular-typing-of-syphilis-causing-strains-among-human-immunodeficiency-virus-positive-patients-in-antwerp-belgium
#13
Lenka Mikalová, Linda Grillová, Kara Osbak, Michal Strouhal, Chris Kenyon, Tania Crucitti, David Šmajs
Centers for Disease Control and Prevention and sequencing-based treponeme typing was used to analyze 72 blood samples, collected from human immunodeficiency virus and syphilis co-infected patients during 2014 to 2015 in Antwerp, Belgium. Twenty-nine (40.3%) isolates were polymerase chain reaction positive for Treponema pallidum, and all tested were macrolide-resistant. Four genotypes were identified by sequencing-based typing including two new genotypes, U4NR8 and SU9R8, whereas enhanced Centers for Disease Control and Prevention typing revealed 7 subtypes...
June 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28453906/oral-treponeme-major-surface-protein-sequence-diversity-and-distributions-within-periodontal-niches
#14
Meng You, Yuki Chan, Donnabella C Lacap-Bugler, Yong-Biao Huo, Wenling Gao, W Keung Leung, Rory M Watt
Treponema denticola and other species (phylotypes) of oral spirochetes are widely-considered to play important etiological roles in periodontitis and other oral infections. The major surface protein (Msp) of T. denticola is directly implicated in several pathological mechanisms. Here, we have analyzed msp sequence diversity across 68 strains of oral phylogroup 1 and 2 treponemes; including reference strains of T. denticola, Treponema putidum, Treponema medium, 'Treponema vincentii', and 'Treponema sinensis'...
April 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28418297/survey-of-treponemal-infections-in-free-ranging-and-captive-macaques-1999-2012
#15
Amy R Klegarth, Chigozie A Ezeonwu, Aida Rompis, Benjamin P Y-H Lee, Nantiya Aggimarangsee, Mukesh Chalise, John Cortes, M Feeroz, Barbara J Molini, Bess C Godornes, Michael Marks, Michael Schillaci, Gregory Engel, Sascha Knauf, Sheila A Lukehart, Lisa Jones-Engel
Survey results showed treponemal infection among pet macaques in Southeast Asia, a region with a high prevalence of human yaws. This finding, along with studies showing treponemal infection in nonhuman primates in Africa, should encourage a One Health approach to yaws eradication and surveillance activities, possibly including monitoring of nonhuman primates in yaws-endemic regions.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28414665/a-case-of-early-neurosyphilis
#16
M Walker, R Wisler, J Simmons, A Johnson
INTRODUCTION: Neurosyphilis is an infection of the central nervous system by Treponema pallidum, which can occur after the initial syphilis infection. Although commonly associated with late stage disease, patients with early neurosyphilis may present with acute syphilitic meningitis, meningovascular syphilis, or uveitis. CASE: A 28 year old man with a past medical history of HIV (CD4 364);, and recent diagnosis of uveitis presented to the Emergency Department with a positive RPR result...
March 2017: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/28399798/the-picasso-cohort-baseline-characteristics-of-a-cohort-of-men-who-have-sex-with-men-and-male-to-female-transgender-women-at-high-risk-for-syphilis-infection-in-lima-peru
#17
Noah Kojima, Hayoung Park, Kelika A Konda, Dvora L Joseph Davey, Claire C Bristow, Brandon Brown, Segundo R Leon, Silver K Vargas, Gino M Calvo, Carlos F Caceres, Jeffrey D Klausner
BACKGROUND: Men who have sex with men (MSM) and male-to-female transgender women (transwomen) are disproportionately at risk of syphilis infection in Peru. METHODS: From 2013 to 2014, MSM and transwomen seeking human immunodeficiency virus (HIV) or sexually transmitted infection (STI) testing and/or treatment were recruited into a 2-year observational cohort study to determine predictors of recently acquired syphilis infection (defined as a rapid plasma reagin [RPR] titer ≥1:16 and a reactive treponemal antibody test) in Lima, Peru...
April 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28399709/treponema-pallidum-ssp-pallidum-identification-by-real-time-pcr-targetting-the-pola-gene-in-paraffin-embedded-samples-positive-by-immunohistochemistry
#18
A Gama, E M Carrillo-Casas, R Hernández-Castro, V A Vázquez-Aceituno, S Toussaint-Caire, J Xicohtencatl-Cortes, R Fernández-Martínez, G Moreno-Coutiño
Syphilis is a systemic and sexually transmitted infection caused by Treponema pallidum ssp. pallidum. This spirochete causes different clinical and subclinical stages depending on the duration of infection and immune status of the host. Several tests have been developed for diagnosis, and are classified into direct and indirect methods. The first one includes dark field microscopy, direct fluorescent antibody test in fluids or tissue, and molecular biology techniques. In the indirect method (serologic), the routine tests are used, and are divided in two categories: non-treponemal and treponemal ones...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28398683/update-on-syphilis-and-pregnancy
#19
REVIEW
Michael E Tsimis, Jeanne S Sheffield
While the origins of syphilis remain unknown, it has long been recognized as an infectious entity with complex pathophysiology. In this review, we highlighted the epidemiology and risk factors associated with syphilis. The incidence of syphilis in most populations showed a consistent upward trend until the 1940s with the introduction of penicillin as the preferred treatment. The emergence of congenital syphilis and vertical transmission has been a direct result of heterosexual syphilis transmission. We also explore the microbiology and pathogenesis of Treponema pallidum as it directly correlates with its route of transmission and infectivity...
March 15, 2017: Birth defects research
https://www.readbyqxmd.com/read/28363959/microbiota-analysis-of-an-environmental-slurry-and-its-potential-role-as-a-reservoir-of-bovine-digital-dermatitis-pathogens
#20
Kirstine Klitgaard, Mikael L Strube, Anastasia Isbrand, Tim K Jensen, Martin W Nielsen
At present, very little information exists regarding what role the environmental slurry may play as an infection reservoir and/or route of transmission for bovine digital dermatitis (DD), a disease which is a global problem in dairy herds. To investigate whether DD-related bacteria belong to the indigenous microbiota of the dairy herd environment, we used deep amplicon sequencing of the 16S rRNA gene in 135 slurry samples collected from different sites in 22 dairy farms, with and without DD-infected cows. Both the general bacterial populations and digital dermatitis-associated Treponema were targeted in this study...
June 1, 2017: Applied and Environmental Microbiology
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