Read by QxMD icon Read

Treponema pallidum

Ennio Polilli, Federica Sozio, Paola Di Stefano, Antonina Sciacca, Tamara Ursini, Maurizio Paoloni, Jacopo Vecchiet, Dante Di Giammartino, Maria Pina Sciotti, Alessandro Grimaldi, Valerio Cortesi, Paolo Fazii, Elena Ricci, Claudio D'Amario, Giuseppe Ippolito, Lucio Pippa, Giustino Parruti
Undiagnosed cases of HIV infection in developed countries are estimated at 20-30% of individuals living with HIV. Web-based strategies may represent a new approach to easier, wider, and unrestricted access to early testing. The Abruzzo Region, Italy, developed a Web-based tool to recruit persons at high risk of HIV and other sexually transmitted infections (STIs). At the Website , browsers found information on STIs (HIV, hepatitis B and C, and syphilis), a structured questionnaire called "risk calculator" to assess one's own risk behaviors and direct booking of their test at one of six sites throughout the region...
October 2016: AIDS Patient Care and STDs
Miguel Pinto, Vítor Borges, Minia Antelo, Miguel Pinheiro, Alexandra Nunes, Jacinta Azevedo, Maria José Borrego, Joana Mendonça, Dina Carpinteiro, Luís Vieira, João Paulo Gomes
Insights into the genomic adaptive traits of Treponema pallidum, the causative bacterium of syphilis, have long been hampered due to the absence of in vitro culture models and the constraints associated with its propagation in rabbits. Here, we have bypassed the culture bottleneck by means of a targeted strategy never applied to uncultivable bacterial human pathogens to directly capture whole-genome T. pallidum data in the context of human infection. This strategy has unveiled a scenario of discreet T. pallidum interstrain single-nucleotide-polymorphism-based microevolution, contrasting with a rampant within-patient genetic heterogeneity mainly targeting multiple phase-variable loci and a major antigen-coding gene (tprK)...
October 17, 2016: Nature Microbiology
Justin D Radolf, Ranjit K Deka, Arvind Anand, David Šmajs, Michael V Norgard, X Frank Yang
The past two decades have seen a worldwide resurgence in infections caused by Treponema pallidum subsp. pallidum, the syphilis spirochete. The well-recognized capacity of the syphilis spirochete for early dissemination and immune evasion has earned it the designation 'the stealth pathogen'. Despite the many hurdles to studying syphilis pathogenesis, most notably the inability to culture and to genetically manipulate T. pallidum, in recent years, considerable progress has been made in elucidating the structural, physiological, and regulatory facets of T...
October 10, 2016: Nature Reviews. Microbiology
Shuguo Yang, Danmei Jiao, Changjun Liu, Ming Lv, Shan Li, Zongyun Chen, Yao Deng, Yanqing Zhao, Jian Li
BACKGROUND: Ordinary screening of transfusion-transmissible infections (TTIs) among blood donors is essential for blood transfusion. Although there is several TTIs studies focus on human immunodeficiency virus, hepatitis B and C viruses, and Treponema pallidum infections in China, it is no data to illustrate any firm conclusion from Shiyan City, Central China. It aims to verify the seroprevalence of TTIs among blood donors at Shiyan. METHODS: A retrospective analysis of blood donors' information was conducted for the presence of HIV, HBV, HCV and T...
October 1, 2016: BMC Infectious Diseases
Zhou Jun, Chen Zhen, Zhang QuiuLi, An YuanQi, Verónica Vocero Casado, Yuan Fan
Currently, conventional enzyme immunoassays which use manual gold, and colloidal tests (GICT) are used as a screening tool to detect Treponema pallidum (syphilis), hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency viruses (HIV 1/2) in patients undergoing surgery. The present observational, cross-sectional study compared the sensitivity, specificity and workflow characteristics of the conventional algorithm using manual GICT with a newly proposed algorithm that uses automated BIO-FLASH® technology as a screening tool in patients undergoing gastrointestinal (GI) endoscopy...
October 5, 2016: Journal of Clinical Microbiology
Kurni Kurniyati, John F Kelly, Evgeny Vinogradov, Anna Robotham, Youbing Tu, Juyu Wang, Jun Liu, Susan M Logan, Chunhao Li
While protein glycosylation has been reported in several spirochetes including the syphilis bacterium Treponema pallidum and Lyme disease pathogen Borrelia burgdorferi, the pertinent glycan structures and their roles remain uncharacterized. Herein, we report a novel glycan with an unusual chemical composition and structure in the oral spirochete Treponema denticola, a keystone pathogen of periodontitis. The identified glycan of mass 450.2 Da is composed of a monoacetylated nonulosonic acid (Non) with a novel extended N7 acyl modification, a 2-methoxy-4,5,6-trihydroxy-hexanoyl residue in which the Non has a pseudaminic acid configuration (L-glycero-L-manno) and is β-linked to serine or threonine residues...
October 1, 2016: Molecular Microbiology
Fan Wu, Jing-Ping Zhang, Qian-Qiu Wang
Treponema pallidum subspecies pallidum (T. pallidum), the pathogen of syphilis, can invade the central nervous system (CNS) and causes a series of severe diseases collectively termed neurosyphilis (NS).(1) Recently, there are increasing reports of NS cases, especially in HIV-infected syphilis patients.(2,3) Meanwhile, the misdiagnosis rate of NS is high, that of neurosyphilitic ischemic stroke was up to 80.95%.(4) This article is protected by copyright. All rights reserved.
September 29, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
Michelle L Parker, Simon Houston, Helena Pětrošová, Karen V Lithgow, Rebecca Hof, Charmaine Wetherell, Wei-Chien Kao, Yi-Pin Lin, Tara J Moriarty, Rhodaba Ebady, Caroline E Cameron, Martin J Boulanger
Syphilis is a chronic disease caused by the bacterium Treponema pallidum subsp. pallidum. Treponema pallidum disseminates widely throughout the host and extravasates from the vasculature, a process that is at least partially dependent upon the ability of T. pallidum to interact with host extracellular matrix (ECM) components. Defining the molecular basis for the interaction between T. pallidum and the host is complicated by the intractability of T. pallidum to in vitro culturing and genetic manipulation. Correspondingly, few T...
September 2016: PLoS Pathogens
Michael R Miller, Kelly A Miller, Jiang Bian, Milinda E James, Sheng Zhang, Michael J Lynch, Patrick S Callery, Justin M Hettick, Andrew Cockburn, Jun Liu, Chunhao Li, Brian R Crane, Nyles W Charon
Spirochaetes are bacteria responsible for several serious diseases, including Lyme disease (Borrelia burgdorferi), syphilis (Treponema pallidum) and leptospirosis (Leptospira interrogans), and contribute to periodontal diseases (Treponema denticola)(1). These spirochaetes employ an unusual form of flagella-based motility necessary for pathogenicity; indeed, spirochaete flagella (periplasmic flagella) reside and rotate within the periplasmic space(2-11). The universal joint or hook that links the rotary motor to the filament is composed of ∼120-130 FlgE proteins, which in spirochaetes form an unusually stable, high-molecular-weight complex(9,12-17)...
2016: Nature Microbiology
Quirijn de Mast, Jessica E Molhoek, André J van der Ven, William K Gray, Philip G de Groot, Ahmed Jusabani, Ferdinand Mugusi, Rolf T Urbanus, Richard W Walker
BACKGROUND AND PURPOSE: The burden of stroke is high in sub-Saharan Africa, and improved knowledge of risk factors is needed. Antiphospholipid antibodies are a common acquired stroke risk factor in young individuals. Antiphospholipid antibodies may be induced by infectious diseases. Sub-Saharan Africa has a high infectious burden, and we analyzed the contribution of antiphospholipid antibodies to the risk of stroke in an incident population from rural and urban Tanzania. METHODS: Stroke cases and age- and sex-matched community-acquired controls from the rural Hai district and urban Dar-es-Salaam areas of Tanzania were recruited in a wider study of stroke incidence between June 2003 and June 2006...
October 2016: Stroke; a Journal of Cerebral Circulation
Kara K Osbak, Simon Houston, Karen V Lithgow, Conor J Meehan, Michal Strouhal, David Šmajs, Caroline E Cameron, Xaveer Van Ostade, Chris R Kenyon, Geert A Van Raemdonck
BACKGROUND: The spirochete bacterium Treponema pallidum ssp. pallidum is the etiological agent of syphilis, a chronic multistage disease. Little is known about the global T. pallidum proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. METHODOLOGY/PRINCIPAL FINDINGS: To better understand the T. pallidum proteome profile during infection, we studied T. pallidum ssp. pallidum DAL-1 strain bacteria isolated from rabbits using complementary mass spectrometry techniques, including multidimensional peptide separation and protein identification via matrix-assisted laser desorption ionization-time of flight (MALDI-TOF/TOF) and electrospray ionization (ESI-LTQ-Orbitrap) tandem mass spectrometry...
September 2016: PLoS Neglected Tropical Diseases
Néstor Casillas-Vega, Rayo Morfín-Otero, Santos García, Jorge Llaca-Díaz, Eduardo Rodríguez-Noriega, Adrián Camacho-Ortiz, Ma de la Merced Ayala-Castellanos, Soraya Mendoza-Olazarán, Samantha Flores-Treviño, Santiago Petersen-Morfín, Héctor J Maldonado-Garza, Francisco J Bosques-Padilla, Elvira Garza-González
OBJECTIVE: To determine the frequency of nine sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico. MATERIALS AND METHODS: Samples from 662 patients attending obstetrics and gynecology clinics were analyzed. Treponema pallidum, HIV, and HCV were detected by serology. HPV was detected by Polimerase Chain Reaction (PCR), and its genotype was determined by Restriction Fragment Length Polymorphism (RFLP)...
August 2016: Salud Pública de México
Clélia Vanhaecke, Philippe Grange, Nadjet Benhaddou, Philippe Blanche, Dominique Salmon, Perrine Parize, Olivier Lortholary, Eric Caumes, Isabelle Pelloux, Olivier Epaulard, Jérôme Guinard, Nicolas Dupin
BACKGROUND:  Syphilis remains a significant public health problem. We conducted a prospective study to define more precisely the clinical and biological characteristics of patients with neurosyphilis (NS), and we assessed the diagnostic value of nested polymerase chain reaction (PCR) testing for Treponema pallidum in cerebrospinal fluid (CSF) samples. METHODS:  From 2001 to 2013, we included 40 patients (90% men; 45% infected with human immunodeficiency virus) with NS, defined as syphilis with neurological and/or ophthalmological symptoms and CSF abnormalities...
November 1, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Nada A Abdelrahim, Hiba I Ahmed, Imad M Fadl-Elmula, Magdi A Bayoumi, Mamoun M Homeida
Sexually transmitted infections (STIs) are major health threats affecting people globally; however, the burden of STIs is greatest in low-income countries. Since they are physiologically more vulnerable, women are mostly affected. The risk is increased dramatically during pregnancy leading to serious health complications that may affect the newborn. Underprivileged pregnant women attending antenatal clinics for routine checkups in displaced camps, a women's prison and several peripheral health centres were clinically and laboratory screened for trichomoniasis, chlamydial infections, gonorrhea and syphilis...
August 31, 2016: International Journal of STD & AIDS
Sho Ogata, Ken Shimizu, Tomohiro Oda, Susumu Tominaga, Kuniaki Nakanishi
Human intestinal spirochetosis (HIS) is a colorectal infection by Brachyspira species of spiral bacteria. Immunohistochemical cross-reaction to an antibody for Treponema pallidum aids its histologic diagnosis. This study's aim was to analyze the immunohistochemical characteristics of HIS. In this analysis, on 223 specimens from 83 HIS cases, we focused on so-called fringe formation (a histologic hallmark of HIS), spiral organisms within mucus or within crypts, and strong immunopositive materials in the mucosa, together with their location and the types of lesions...
August 28, 2016: Human Pathology
Francesco Drago, Giulia Ciccarese, Carlo F Tomasini, Paola Calamaro, Maurizio Boggio, Alfredo Rebora, Aurora Parodi
We describe herein a woman who developed subcutaneous gummas in her trochanteric regions, bilaterally, although she had been treated for syphilis two decades earlier. Evidence of Treponema pallidum latent late infection was the presence of IgG antibodies against T. pallidum and the positive non-treponemal and treponemal tests. Moreover, immunohistochemical staining for T. pallidum detected some spirochetes close to the atrophic adipocytes allowing the diagnosis of lypo-atrophic panniculitis tertiary syphilis...
August 24, 2016: International Journal of STD & AIDS
Michael Marks, Oliver Sokana, Eli Nachamkin, Elliot Puiahi, Georgina Kilua, Allan Pillay, Christian Bottomley, Anthony W Solomon, David C Mabey
INTRODUCTION: Both yaws and trachoma are endemic in the Pacific. Mass treatment with azithromycin is the mainstay of the WHO strategy for both the eradication of yaws and the elimination of trachoma as a public health problem, but the dose recommended for trachoma is lower than that for yaws. In countries where both diseases are endemic, there is a potential for synergy between yaws and trachoma control programs if mass treatment with the lower dose of azithromycin was shown to be effective for the treatment of yaws...
August 2016: PLoS Neglected Tropical Diseases
Chad A Brautigam, Ranjit K Deka, Wei Z Liu, Michael V Norgard
Treponema pallidum, the bacterium that causes syphilis, is an obligate human parasite. As such, it must acquire energy, in the form of carbon sources, from the host. There is ample evidence that the principal source of energy for this spirochete is D-glucose acquired from its environment, likely via an ABC transporter. Further, there is genetic evidence of a D-glucose chemotaxis system in T. pallidum. Both of these processes may be dependent on a single lipidated chemoreceptor: Tp0684, also called TpMglB-2 for its sequence homology to MglB of Escherichia coli...
2016: PloS One
A V Runina, A S Starovoitova, D G Deryabin, A A Kubanov
UNLABELLED: BACKGRAUND. Treponemal tests based on the detection of antibodies against the Treponema pallidum antigens are the most specific methods for serological diagnosis of syphilis. Due to the inability to cultivate this bacterium in vitro, the most promising sources of antigens for diagnostics are recombinant proteins of T. pallidum. Evaluation of the analytical value of certain T. pallidum proteins is the approach to improve sensitivity, specificity, and reproducibility of syphilis serological tests, including possibilities of differential diagnosis of various forms of the disease...
2016: Vestnik Rossiĭskoĭ Akademii Meditsinskikh Nauk
Barbara J Molini, Lauren C Tantalo, Sharon K Sahi, Veronica I Rodriguez, Stephanie L Brandt, Mark C Fernandez, Charmie B Godornes, Christina M Marra, Sheila A Lukehart
BACKGROUND: High rates of 23S rDNA mutations implicated in macrolide resistance have been identified in Treponema pallidum samples from syphilis patients in many countries. Nonetheless, some clinicians have been reluctant to abandon azithromycin as a treatment for syphilis, citing the lack of a causal association between these mutations and clinical evidence of drug resistance. Although azithromycin resistance has been demonstrated in vivo for the historical Street 14 strain, no recent T...
September 2016: Sexually Transmitted Diseases
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"