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Treponema pallidum

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https://www.readbyqxmd.com/read/29136744/-factors-associated-with-syphilis-hiv-infection-among-men-who-have-sex-with-men-in-shenzhen-2011-2016
#1
Y M Cai, Y J Song, H Liu, F C Hong
Objective: To investigate the factors associated with syphilis/HIV infection among men who have sex with men (MSM) maintaining a single sexual partner in Shenzhen. Methods: Respondent driven sampling and snowball sampling method were used to recruit MSM receiving voluntary counseling and testing in Shenzhen Rainbow clinic from 2011 to 2016. INCLUSION CRITERIA: aged 18 years and above; reported having one or more anal sexual partners in the recent 6 months. A total of 3 109 men who have sex with men (MSM) were involved in the study...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29135519/syphilis-of-the-aerodigestive-tract
#2
Julie Y Tse, May P Chan, Judith A Ferry, Vikram Deshpande, Aliyah R Sohani, Valentina Nardi, Andras Schaffer, Rosalynn M Nazarian, Lawrence R Zukerberg
Syphilis, a sexually transmitted infection caused by the Gram-negative bacterium Treponema pallidum, is increasing in prevalence in the United States. It has been our experience that primary and secondary syphilis of the aerodigestive tract can afflict a large age spectrum with varied clinical and histopathologic findings, which can lead to diagnostic problems and frequent misdiagnosis. In this study, we describe the histopathologic patterns of syphilis of the aerodigestive tract to expand awareness of its varied appearance...
November 9, 2017: American Journal of Surgical Pathology
https://www.readbyqxmd.com/read/29133803/microbial-diversity-of-genital-ulcers-of-hsv-2-seropositive-women
#3
Supriya D Mehta, Ashish K Pradhan, Stefan J Green, Ankur Naqib, Elijah Odoyo-June, Charlotte A Gaydos, Sheila Barry, Alan Landay, Robert C Bailey
We measured the microbial community structure of genital ulcers in women. Swabs from clinically detected ulcers were tested for HSV-2 and Treponema pallidum by polymerase chain reaction (PCR). HSV-2 and T. pallidum were detected by serum antibody testing. Microbial community structure was characterized by high-throughput 16 s rRNA gene amplicon sequencing. Multiple group testing and Elastic net and Lasso regressions identified taxa associated with differences in factors of interest. Among 49 ulcer specimens from 49 HSV-2 seropositive women, by PCR HSV-2 was recovered from 28 (57%) specimens and T...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29124540/oligonucleotide-microchip-for-the-identification-of-infectious-agents-of-reproductive-system-with-simultaneous-analysis-of-determinants-of-resistance-to-antimicrobial-substances
#4
A T Leinsoo, B L Shaskol'skii, E I Dement'eva, D A Gryadunov, A A Kubanov, A V Chestkov, O A Obraztsova, M V Shpilevaya, D G Deryabin
We developed a multiplexed DNA microarray-based assay allowing identification of 12 causative agents of reproductive tract infections with the simultaneous detection of 47 genetic determinants of resistance to antimicrobial substances. The microarray was tested on 93 isolates of Neisseria gonorrhoeae, 32 isolates of Treponema pallidum and 29 samples of Ureaplasma spp./Mycoplasma spp. The N. gonorrhoeae isolates had multiple mutations in the penA, ponA, rpsJ, gyrA, parC, and mtrR genes; their prognostic value significantly increased when combinations of mutations were detected...
November 10, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29117430/biological-false-positive-venereal-disease-research-laboratory-test-in-cerebrospinal-fluid-in-the-diagnosis-of-neurosyphilis-a-case-control-study
#5
S Zheng, R J Lin, Y H Chan, Ccl Ngan
BACKGROUND: There is no clear consensus on the diagnosis of neurosyphilis. The Venereal Disease Research Laboratory (VDRL) test from cerebrospinal fluid (CSF) has traditionally been considered the gold standard for diagnosing neurosyphilis, but is widely known to be insensitive. OBJECTIVE: In this study, we compared the clinical and laboratory characteristics of true positive VDRL-CSF cases with biological false positive VDRL-CSF cases. METHODS: We retrospectively identified cases of true and false positive VDRL-CSF across a 3-year period received by the Immunology and Serology Laboratory, Singapore General Hospital...
November 8, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29107621/effectiveness-of-single-dose-azithromycin-to-treat-latent-yaws-a-longitudinal-comparative-cohort-study
#6
Oriol Mitjà, Camila González-Beiras, Charmie Godornes, Reman Kolmau, Wendy Houinei, Haina Abel, August Kapa, Raymond Paru, Sibauk V Bieb, James Wangi, Sergi Sanz, Kingsley Asiedu, Sheila A Lukehart, Quique Bassat
BACKGROUND: Treatment of latent yaws is a crucial component of the WHO yaws eradication strategy to prevent relapse and the resulting transmission to uninfected children. We assessed the effectiveness of single-dose azithromycin to treat patients with latent yaws. METHODS: This population-based cohort study included children (age <20 years) living on Lihir Island, Papua New Guinea, with high-titre (rapid plasma reagin titre ≥1:8) latent or active yaws, between April, 2013, and May, 2015...
October 26, 2017: Lancet Global Health
https://www.readbyqxmd.com/read/29099372/rare-isolated-oral-manifestations-of-secondary-syphilis-in-an-adolescent
#7
Cínthia M Ribeiro, Marina L de Carli, Felipe F Sperandio, Evandro M S Magalhães, João Adolfo C Hanemann
Syphilis is a sexually transmitted infection caused by Treponema pallidum. The disease can manifest clinically through 3 different stages: primary, secondary, and tertiary. This report describes an uncommon case of secondary syphilis in a 16-year-old boy who showed only oral clinical manifestations. The infection was diagnosed through serologic tests. Treatment with intramuscular benzathine penicillin G led to complete remission of the oral lesions 3 weeks after initiation of treatment. The current case demonstrates the importance of laboratory tests in establishing an accurate diagnosis of syphilis and thereby avoiding unnecessary biopsies...
November 2017: General Dentistry
https://www.readbyqxmd.com/read/29090453/cross-sectional-study-of-treponema-pallidum-pcr-in-diagnosis-of-primary-and-secondary-syphilis
#8
Miguel Costa-Silva, Daniel Coutinho, Joana Sobrinho-Simões, Filomena Azevedo, Carmen Lisboa
BACKGROUND: Syphilis remains a major challenge and a complex diagnosis. We aim to evaluate the role of polymerase chain reaction (PCR) in Treponema pallidum (Tp) detection in various types of biological samples in the diagnosis of early syphilis. METHODS: We conducted a cross-sectional study including all attendees of the STI clinic with clinical suspicion of early syphilis. One or more specimens for the detection of Tp by PCR testing were collected. RESULTS: The overall sensitivity of Tp PCR test was 82...
October 31, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/29077095/caspase-4-activation-by-a-bacterial-surface-protein-is-mediated-by-cathepsin-g-in-human-gingival-fibroblasts
#9
Hye-Kyoung Jun, Young-Jung Jung, Suk Ji, Sun-Jin An, Bong-Kyu Choi
Caspase-4 is an inflammatory caspase; however, its mechanism of activation is poorly understood. In this study, we demonstrate that Td92, a surface protein of the periodontal pathogen Treponema denticola and a homolog of the Treponema pallidum surface protein Tp92, activates caspase-4 and induces pyroptosis in primary cultured human gingival fibroblasts (HGFs) via cathepsin G activation. Cathepsin G inhibition or siRNA knockdown of cathepsin G inhibited Td92-induced caspase-4 activation and cell death. Td92-induced cell death was significantly inhibited by siRNA knockdown of gasdermin D...
October 27, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29073145/the-cost-and-cost-effectiveness-of-rapid-testing-strategies-for-yaws-diagnosis-and-surveillance
#10
Christopher Fitzpatrick, Kingsley Asiedu, Anita Sands, Tita Gonzalez Pena, Michael Marks, Oriol Mitja, Filip Meheus, Patrick Van der Stuyft
BACKGROUND: Yaws is a non-venereal treponemal infection caused by Treponema pallidum subspecies pertenue. The disease is targeted by WHO for eradication by 2020. Rapid diagnostic tests (RDTs) are envisaged for confirmation of clinical cases during treatment campaigns and for certification of the interruption of transmission. Yaws testing requires both treponemal (trep) and non-treponemal (non-trep) assays for diagnosis of current infection. We evaluate a sequential testing strategy (using a treponemal RDT before a trep/non-trep RDT) in terms of cost and cost-effectiveness, relative to a single-assay combined testing strategy (using the trep/non-trep RDT alone), for two use cases: individual diagnosis and community surveillance...
October 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29053737/screening-for-hiv-hepatitis-b-and-syphilis-on-dried-blood-spots-a-promising-method-to-better-reach-hidden-high-risk-populations-with-self-collected-sampling
#11
Inge H M van Loo, Nicole H T M Dukers-Muijrers, Rosalie Heuts, Marianne A B van der Sande, Christian J P A Hoebe
INTRODUCTION: Many people at high risk for sexually transmitted infections (STIs), e.g., men who have sex with men (MSM), are not optimally reached by current sexual health care systems with testing. To facilitate testing by home-based sampling or sampling in outreach setting we evaluated dried blood spots (DBS), a method for self-collected blood sampling for serological screening of HIV, hepatitis B (HBV) and syphilis. The aims of this study were to assess the acceptability and feasibility of self-collected DBS and to compare the test results for screening of HIV, HBV and syphilis from DBS with blood drawn by venous puncture...
2017: PloS One
https://www.readbyqxmd.com/read/29051759/ifn%C3%AE-enhances-cd64-potentiated-phagocytosis-of-treponema-pallidum-opsonized-with-human-syphilitic-serum-by-human-macrophages
#12
Kelly L Hawley, Adriana R Cruz, Sarah J Benjamin, Carson J La Vake, Jorge L Cervantes, Morgan LeDoyt, Lady G Ramirez, Daniza Mandich, Mary Fiel-Gan, Melissa J Caimano, Justin D Radolf, Juan C Salazar
Syphilis is a multi-stage, sexually transmitted disease caused by the spirochete Treponema pallidum (Tp). Considered broadly, syphilis can be conceptualized as a dualistic process in which spirochete-driven inflammation, the cause of clinical manifestations, coexists to varying extents with bacterial persistence. Inflammation is elicited in the tissues, along with the persistence of spirochetes to keep driving a robust immune response while evading host defenses; this duality is best exemplified during the florid, disseminated stage called secondary syphilis (SS)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29050149/-accuracy-analysis-and-comparison-of-different-serological-detection-methods-in-syphilis
#13
M Hu, N Liu, Y Y Cai, T Lei, M Zhang
Objective: To compare the accuracy of different methods by reviewing syphilis serologic screening external quality assessment (EQA) data of Chinese primary health care institutions. Methods: The EQA was based on one or two distinct shipments from 2011 to 2016. Each shipment consisted of five serum samples. The laboratories performed the EQA and sent back the results by the deadline. The results were analyzed using the standard procedures and methods. Results: Six different methods were used for syphilis serologic screening: enzyme linked immunosorbent assay, colloidal gold method, Treponema pallidum particle agglutination (TPPA) test, rapid plasma reagin card test, toluidine red unheated serum reagin test and chemiluminescence immunoassay...
September 26, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29046410/correlation-of-treponemal-immunoassay-signal-strength-values-with-reactivity-of-confirmatory-treponemal-testing
#14
Yetunde F Fakile, Heather Jost, Karen W Hoover, Kathleen J Gustafson, Susan M Novak-Weekley, Jeff M Schapiro, Anthony Tran, Joan M Chow, Ina U Park
Automated treponemal immunoassays are used for syphilis screening with the reverse sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA]-reactive, reactive plasma reagin [RPR]-non-reactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal strength values consistently correlating with reactive confirmatory treponemal testing.We conducted a cross-sectional analysis of four automated immunoassays: BioPlex 2200 microbead immunoassay (MBIA), LIAISON chemiluminesence immunoassay (CIA), ADVIA-Centaur CIA, and TrepSure EIA, and three manual assays: Treponema Pallidum Particle Agglutination (TP-PA), Fluorescent Treponemal Antibody-Absorption (FTA-ABS) test, INNO-LIA line immunoassay...
October 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29045605/diagnostics-for-yaws-eradication-insights-from-direct-next-generation-sequencing-of-cutaneous-strains-of-treponema-pallidum
#15
Michael Marks, Maria Fookes, Josef Wagner, Robert Butcher, Rosanna Ghinai, Oliver Sokana, Yaw-Adu Sarkodie, Sheila A Lukehart, Anthony W Solomon, David C W Mabey, Nicholas Thomson
Background: Yaws-like chronic ulcers can be caused by Treponema pallidum subsp. pertenue, Haemophilus ducreyi, or other still-undefined bacteria. To permit accurate evaluation of yaws elimination efforts programmatic use of molecular diagnostics is required. The accuracy and sensitivity of current tools remains unclear because our understanding of T. pallidum diversity is limited by the low number of sequenced genomes. Methods: We tested samples from patients with suspected yaws collected in previous studies in the Solomon Islands and Ghana...
October 16, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29022569/syphilis
#16
REVIEW
Rosanna W Peeling, David Mabey, Mary L Kamb, Xiang-Sheng Chen, Justin D Radolf, Adele S Benzaken
Treponema pallidum subspecies pallidum (T. pallidum) causes syphilis via sexual exposure or via vertical transmission during pregnancy. T. pallidum is renowned for its invasiveness and immune-evasiveness; its clinical manifestations result from local inflammatory responses to replicating spirochaetes and often imitate those of other diseases. The spirochaete has a long latent period during which individuals have no signs or symptoms but can remain infectious. Despite the availability of simple diagnostic tests and the effectiveness of treatment with a single dose of long-acting penicillin, syphilis is re-emerging as a global public health problem, particularly among men who have sex with men (MSM) in high-income and middle-income countries...
October 12, 2017: Nature Reviews. Disease Primers
https://www.readbyqxmd.com/read/29020212/screening-veterans-for-syphilis-implementation-of-the-reverse-sequence-algorithm
#17
Meredith E Clement, Amr Hammouda, Lawrence P Park, Jason Maxwell, Erika Samoff, Arlene C Seña, Maria Joyce
We evaluated the syphilis reverse sequence algorithm (RSA) in a Veteran Affairs facility, finding 5.5% reactive Treponema pallidum enzyme immunoassay (EIA) tests. In a subset of EIA+/VDRL-/TP-PA+ cases, 48% were previously treated. Of veterans with unknown/no prior therapy, only 45% had documentation of subsequent treatment, suggesting suboptimal interpretation of RSA results.
August 2, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29020192/single-dose-azithromycin-for-the-treatment-of-haemophilus-ducreyi-skin-ulcers-in-papua-new-guinea
#18
Camila González-Beiras, August Kapa, Marti Vall Mayans, Raymond Paru, Sergi Gavilán, Wendy Houinei, Sibauk Bieb, Sergi Sanz, Rosario Martíns, Oriol Mitjà
Background: Haemophilus ducreyi (HD) and Treponema pallidum subsp. pertenue (TP) are major causative agents of cutaneous ulcers (CU) in the tropics. Azithromycin is recommended to treat sexually transmitted HD infections and has good in-vitro activity against HD strains from both genital and skin ulcers. We investigated the efficacy of single oral dose azithromycin on HD-CU. Methods: We conducted a community-based cohort study in Lihir Island, Papua New Guinea, from October 2014 through May 2016...
August 16, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29020152/increases-in-ocular-syphilis-north-carolina-2014-2015
#19
Sara E Oliver, Anna Barry Cope, Jessica L Rinsky, Charnetta Williams, Gui Liu, Stephanie Hawks, Thomas A Peterman, Lauri Markowitz, Aaron T Fleischauer, Erika Samoff
Background: Ocular syphilis is an inflammatory eye disease due to Treponema pallidum infection. In the United States, syphilis rates have increased since 2000; clusters of ocular syphilis were reported in 2015. We investigated ocular syphilis in North Carolina to describe the epidemiology and clinical course of disease. Methods: We reviewed syphilis cases reported to North Carolina during 2014-2015 and abstracted information from health department interviews for cases with ocular symptoms and no other defined etiology...
July 13, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29016285/development-and-evaluation-of-a-loop-mediated-isothermal-amplification-assay-for-the-detection-of-treponema-pallidum-dna-in-the-peripheral-blood-of-secondary-syphilis-patients
#20
Yongjian Xiao, Yafeng Xie, Man Xu, Shuangquan Liu, Chuanhao Jiang, Feijun Zhao, Tiebing Zeng, Zhuoran Liu, Jian Yu, Yimou Wu
Secondary syphilis (SS) has always been puzzling for the clinicians because of the similarity of the appearance of skin rashes with other dermatoses. Serological assays are useful, but less sensitive at an early stage of SS or when patients are immunodeficient. Therefore, there is an urgent need to develop a rapid and effective tool for the diagnosis of SS, which may play an important role in the control of epidemic syphilis outbreaks. In this study, we evaluated a loop-mediated isothermal amplification (LAMP) assay, targeting gene encoding the basic membrane protein of Treponema pallidum, to detect the presence of circulating T...
October 2, 2017: American Journal of Tropical Medicine and Hygiene
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