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https://www.readbyqxmd.com/read/27914222/molecular-diversity-of-human-parvovirus-b19-during-two-outbreaks-of-erythema-infectiosum-in-brazil
#1
Rita de Cássia Nasser Cubel Garcia, Renata Freire Alves Pereira, Kátia Martins Lopes de Azevedo, Tatiana Xavier de Castro, Francisco C A Mello, Sérgio Setubal, Marilda M Siqueira, David Brown, Solange Artimos de Oliveira
This study was conducted to provide information on the genetic diversity of human parvovirus B19 (B19V) circulating in the municipality of Niterói, Rio de Janeiro, Southeast Brazil during 1996-2006, a period with two distinct outbreaks of B19V infection: 1999-2000 and 2004-2005. A total of 27 sera from patients with erythema infectiosum (EI) and five sera from HIV-infected patients that tested positive for B19V DNA during the study period were analyzed. To genotype B19V strains, a semi-nested PCR for partial amplification of the capsid gene was performed and sequence analysis revealed that 31 sequences belonged to subgenotype 1a (G1a) of the main genotype 1 and one sequence was characterized as subgenotype 3b (G3b)...
November 30, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27914203/multiple-processes-drive-genetic-structure-of-humpback-whale-megaptera-novaeangliae-populations-across-spatial-scales
#2
Francine Kershaw, Inês Carvalho, Jacqueline Loo, Cristina Pomilla, Peter B Best, Ken P Findlay, Salvatore Cerchio, Tim Collins, Marcia H Engel, Gianna Minton, Peter Ersts, Jaco Barendse, P G H Kotze, Yvette Razafindrakoto, Solange Ngouessono, Michael Meÿer, Meredith Thorton, Howard C Rosenbaum
Elucidating patterns of population structure for species with complex life histories, and disentangling the processes driving such patterns, remains a significant analytical challenge. Humpback whale (Megaptera novaeangliae) populations display complex genetic structures that have not been fully resolved at all spatial scales. We generated a data set of nuclear markers for 3,575 samples spanning the seven breeding stocks and substocks found in the South Atlantic and western and northern Indian Oceans. For the total sample, and males and females separately, we assessed genetic diversity, tested for genetic differentiation between putative populations and isolation by distance, estimated the number of genetic clusters without a priori population information, and estimated rates of gene flow using maximum likelihood and Bayesian approaches...
December 3, 2016: Molecular Ecology
https://www.readbyqxmd.com/read/27908670/proteome-of-the-rete-testis-fluid-from-tropically-adapted-morada-nova-rams
#3
Mauricio Fraga van Tilburg, Solange Damasceno Sousa, Révila Bianca Ferreira de Melo, Frederico B Moreno, Ana Cristina Monteiro-Moreira, Renato A Moreira, Arlindo de Alencar Moura
The rete testis has a close relationship with sperm development and may have other functions besides serving as an intercalated channel. The aim of this study was to identify and characterize the proteins of rete testis fluid (RTF) from tropically-adapted Morada Nova rams. Testicles obtained from six Morada Nova rams were dissected and the head of the epididymis was separated to access the efferent ducts. Rete testis fluid was obtained by gentle massage of the testis. The fluid was centrifuged to remove cell debris and sperm...
November 22, 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27904921/survey-of-plant-growth-promoting-mechanisms-in-native-portuguese-chickpea-mesorhizobium-isolates
#4
Clarisse Brígido, Bernard R Glick, Solange Oliveira
Rhizobia may possess other plant growth-promoting mechanisms besides nitrogen fixation. These mechanisms and the tolerance to different environmental factors, such as metals, may contribute to the use of rhizobia inocula to establish a successful legume-rhizobia symbiosis. Our goal was to characterize a collection of native Portuguese chickpea Mesorhizobium isolates in terms of plant growth-promoting (PGP) traits and tolerance to different metals as well as to investigate whether these characteristics are related to the biogeography of the isolates...
December 1, 2016: Microbial Ecology
https://www.readbyqxmd.com/read/27901255/assessment-and-nutrition-education-in-patients-with-amyotrophic-lateral-sclerosis
#5
Claudinéa S Almeida, Patricia Stanich, Cristina C S Salvioni, Solange Diccini
Neurological patients with amyotrophic lateral sclerosis (ALS)often deteriorate to a worsening nutritional status. The aim of this study was to compare the nutritional status and food intake after nutrition education in patients with ALS. Clinical, anthropometric and functional variables were analyzed. Fifty-three patients were monitored at an early stage of the disease. The average score on the functionality scale was 33 points. Initially only 3.8% were classified as low body weight. After three months, 50% showed significant variation in anthropometric measures related to muscle mass and body fat reserves without association with clinical variables...
November 2016: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/27899107/efficacy-evaluation-of-the-school-program-unplugged-for-drug-use-prevention-among-brazilian-adolescents
#6
Zila M Sanchez, Adriana Sanudo, Solange Andreoni, Daniela Schneider, Ana Paula D Pereira, Fabrizio Faggiano
BACKGROUND: Most Brazilian schools do not have a continuous program for drug use prevention and do not conduct culturally adapted activities for that purpose. This study evaluated the impact of the Unplugged program on drug use prevention among children and adolescents in public middle schools of Brazil. METHODS: A non-randomized controlled trial was conducted in 2013 with 2185 students in 16 public schools from 3 Brazilian cities. The intervention group attended 12 weekly classes of the Unplugged program for drug use prevention, and the control group did not attend to any school prevention programs in the same year...
November 29, 2016: BMC Public Health
https://www.readbyqxmd.com/read/27894694/the-cytokinesis-block-micronucleus-cbmn-assay-in-human-populations-exposed-to-styrene-a-systematic-review-and-meta-analysis
#7
REVIEW
Solange Costa, Marcello Ceppi, Carla Costa, Susana Silva, Cristiana Pereira, Blanca Laffon, Stefano Bonassi, João Paulo Teixeira
Styrene is a building-block of several compounds used in a wide array of materials and products. The most important human exposure to this substance occurs in industrial settings, especially among reinforced-plastics industry workers. The effect of occupational exposure to styrene on cytogenetics biomarkers has been previously reviewed with positive association observed for chromosomal aberrations, and inconclusive data for the micronucleus assay. Some limitations were noted in those studies, including inadequate exposure assessment and poor epidemiological design...
October 2016: Mutation Research
https://www.readbyqxmd.com/read/27883192/a-call-to-action-to-reshape-evidence-synthesis-and-use-for-nutrition-policy
#8
EDITORIAL
Mark Lawrence, Celeste Naude, Rebecca Armstrong, Lisa Bero, Namukolo Covic, Solange Durao, Davina Ghersi, Geraldine Macdonald, Harriet MacLehose, Barrie Margetts, David Tovey, Jimmy Volmink, Taryn Young
No abstract text is available yet for this article.
November 21, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27863213/tubular-cytoplasmic-expression-of-snai1-in-renal-transplant-biopsies-a-sign-of-diseased-epithelial-phenotype
#9
Marco Prunotto, Lyubov Chaykovska, Massimo Bongiovanni, Milo Frattini, Thomas Cagarelli, Franziska Weibel, Maurizio Bruschi, Antonio Garcia de Herreros, Solange Moll
The aim of the present study was to analyze in vivo the role of SNAI1 on renal fibrosis. Unilateral ureteral obstruction injury was induced in Snai1 knockout mice. Snai1 gene deletion was, however, only partial and could therefore not be correlated to reduced fibrosis. Expression of SNAI1 protein and epithelial-mesenchymal transformation markers was then assessed in human chronic allograft nephropathy biopsy specimens. Significant up-regulation of SNAI1 protein was detected within cytoplasm of proximal tubules localized, for some of them, near foci of fibrosis and tubular atrophy...
November 15, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27863145/dietary-and-non-dietary-triggers-of-oral-ulcer-recurrences-in-beh%C3%A3-et-s-disease
#10
Geoffroy Volle, Jean-Baptiste Fraison, Delphine Gobert, Tiphaine Goulenok, Robin Dhote, Olivier Fain, Solange Gonzalez-Chiappe, François Lhote, Thomas Papo, Aurélie Thuillier, Sophie Rivière, Alfred Mahr
OBJECTIVES: The nature and impact of food and other external triggers in recurrences of Behçet's disease (BD)-related oral ulcers (OUs) remain unknown. This survey investigated dietary and non-dietary triggers of BD-related OU recurrences. METHODS: Patients with BD who were followed in 7 French hospital departments completed a self-administered patient questionnaire. General and specific dietary triggering factors were sought in open questions. The questionnaire also included closed questions, notably to evaluate the effect of 6 general triggering situations and 24 selected foods...
November 18, 2016: Arthritis Care & Research
https://www.readbyqxmd.com/read/27849286/perception-of-nurses-about-patient-safety-in-intensive-care-units
#11
Theo Duarte da Costa, Pétala Tuani Candido de Oliveira Salvador, Cláudia Cristiane Filgueira Martins Rodrigues, Kisna Yasmin Andrade Alves, Francis Solange Vieira Tourinho, Viviane Euzébia Pereira Santos
Objective: To understand the perception of nursing professionals in public hospital intensive therapy units regarding patient safety. Methods: This is a descriptive study with a qualitative approach. Eighty nursing professionals who developed welfare activities in general intensive care units in Northeastern Brazil participated. Data were collected through semi-structured interviews, from May to June 2014. The textual content was submitted to the lexicographical textual analysis, and data analysis was based on the literature...
November 16, 2016: Revista Gaúcha de Enfermagem
https://www.readbyqxmd.com/read/27825892/quality-and-reporting-standards-of-south-african-primary-care-clinical-practice-guidelines
#12
Shingai Machingaidze, Babalwa Zani, Amber Abrams, Solange Durao, Quinette Louw, Tamara Kredo, Karen Grimmer, Taryn Young
OBJECTIVES: Clinical Practice Guideline (CPGs) development has evolved over the past decade, with greater emphasis now being placed on transparency, rigour of development, and reporting standards. Our evaluation assesses the quality of the guideline development processes and reporting of selected South African primary care CPGs. STUDY DESIGN: CPGs were iteratively identified by two authors, seeking CPGs reflecting common conditions with which patients present in South African primary care settings...
November 4, 2016: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/27813508/-recurrent-pancreatitis-secondary-to-sphincter-of-oddi-dysfunction-case-report
#13
Jeannette Calzadilla, Natalia Sanhueza, Solange Farías, Félix González
Dysfunction of the sphincter of Oddi is a rare disease that causes about 20% of recurrent idiopathic pancreatitis. In order to suspect its existence, it is mandatory to have ruled out all other causes of acute pancreatitis. Then, the disease needs to be classified by degree of dysfunction and have a manometry performed as it is considered the gold standard of diagnosis. Manometry is used to guide treatment and its method of choice is usually an endoscopic sphincterotomy. In this article a case of recurrent pancreatitis is discussed, which after a thorough study that ruled out other etiologies, was diagnosed as a pancreatic-type dysfunction of the sphincter of Oddi...
October 21, 2016: Medwave
https://www.readbyqxmd.com/read/27813504/todani-choledochal-cyst-type-ii-case-report-and-review
#14
Solange Marcela Farías Molina, Rafael Lisandro Castillo Machado, Natalia Carolina Sanhueza Palma, Jeannette Alejandra Calzadilla Riveras
: Choledochal cyst is a rare pathological condition, which represents 1% of benign diseases of the biliary tract. It is seen with significant frequency in childhood, with a higher prevalence in females, and a greater diagnostic incidence in Asia. Diagnosis is primarily based on clinical suspicion. Its symptoms are variable, and its treatment, depending on the type, is surgical. CASE REPORT: a male patient, 46 years old, with clinical symptoms of acute pancreatitis as a complication of a choledochal cyst Todani type II is diagnosed during his hospital stay with cholangial magnetic resonance...
October 20, 2016: Medwave
https://www.readbyqxmd.com/read/27801902/fitness-costs-restrict-niche-expansion-by-generalist-niche-constructing-pathogens
#15
Julien Lang, Armelle Vigouroux, Abbas El Sahili, Anthony Kwasiborski, Magali Aumont-Nicaise, Yves Dessaux, Jacqui Anne Shykoff, Solange Moréra, Denis Faure
We investigated the molecular and ecological mechanisms involved in niche expansion, or generalism, versus specialization in sympatric plant pathogens. Nopaline-type and octopine-type Agrobacterium tumefaciens engineer distinct niches in their plant hosts that provide different nutrients: nopaline or octopine, respectively. Previous studies revealed that nopaline-type pathogens may expand their niche to also assimilate octopine in the presence of nopaline, but consequences of this phenomenon on pathogen dynamics in planta were not known...
November 1, 2016: ISME Journal
https://www.readbyqxmd.com/read/27796672/three-consecutive-days-of-application-of-led-therapy-is-necessary-to-inhibit-experimentally-induced-root-resorption-in-rats-a-microtomographic-study
#16
Dayla Thyeme Higashi, Avacir Casanova Andrello, Pedro Marcelo Tondelli, Dari de Oliveira Toginho Filho, Solange de Paula Ramos
Previous studies have suggested that phototherapy may modulate orthodontic tooth movement and the incidence of root resorption. We aimed to identify a minimal dose-response relationship to LED therapy with regard to orthodontic tooth movement (OTM) and root resorption in rats. Forty-eight male Wistar rats were divided into six groups with equal and random distribution: control (C) no intervention; three daily LED irradiation (CLED); submitted only to OTM (RR); OTM and LED irradiation on the first day (LED1); OTM and two LED irradiation on the first and second days (LED2); and OTM and three LED irradiation on the first, second, and third days (LED3)...
October 29, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27788612/a-european-survey-of-perendoscopic-treatment-of-biliary-complications-in-patients-with-alveolar-echinococcosis
#17
Sylvain Ambregna, Stéphane Koch, Michael C Sulz, Beate Grüner, Sümeyra Öztürk, Jean-Baptiste Chevaux, Małgorzata Sulima, Andrea de Gottardi, Bertrand Napoléon, Armand Abergel, Philippe Bichard, Isabelle Boytchev, Pierre Deprez, Jerome Dumortier, Jean-Louis Frossard, Eric Kull, Bernard Meny, Darius Moradpour, Fréderic Prat, Geoffroy Vanbiervliet, Thierry Thevenot, Dominique Angèle Vuitton, Solange Bresson-Hadni, Lucine Vuitton
BACKGROUND: Biliary complications represent a turning point in the course of Alveolar Echinococcosis (AE). We conducted a European survey to collect data on the current usage and results of perendoscopic interventions (PEIs) for their treatment. METHODS: Patient's characteristics and follow-up until January 31st, 2015 were recorded using an online questionnaire. RESULTS: From 18 centers 129 PEIs were analyzed in 38 patients; 139 plastic stents were inserted during 85 PEIs; median time between stent placements was significantly longer when 3 stents or more were placed...
October 28, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27784255/clear-shot-at-primary-aim-susceptibility-of-trypanosoma-cruzi-organelles-structures-and-molecular-targets-to-drug-treatment
#18
Rubem Figueiredo Sadok Menna-Barreto, Solange Lisboa de Castro
Chagas disease, caused by Trypanosoma cruzi, stands out due to its socio- economic effects on low-income tropical populations. This disease affects millions of people worldwide. The current chemotherapy for it is based on benznidazole (Bz) and nifurtimox (Nif) and is unsatisfactory. In this review, we will focus on the search for potential target organelles and molecules for the chemotherapy of Chagas disease. We consider as potential target organelles those that are absent or significantly different in host cells and present in the clinically relevant forms of the parasite (trypomastigotes and amastigotes), which are the mitochondrion, cytoskeletal- related structures, the acidocalcisomes/contractile vacuole complex and glycosomes...
October 25, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/27775935/pet-ct-as-an-alternative-to-fiducial-markers-guidance-in-liver-metastasis-treatment
#19
Fernanda Granato, Andre C Felicio, Lilian Yuri Itaya Yamaga, Solange Amorim Nogueira, Taise Vitor
The liver is the organ most commonly affected by colorectal cancer metastasis. Currently, liver resection is the treatment of choice, but in cases where it is not possible, intensity-modulated radiation therapy is an alternative. We report the case of a 57-year-old woman whose colorectal carcinoma metastasized to the liver. She was referred for implantation of fiducial markers for intensity-modulated radiation therapy planning. PET-CT stands out as an additional tool for distinguishing metabolically active tumor tissue from other tissues, ensuring effective introduction of percutaneous fiducial markers, especially in cases where use of conventional imaging techniques is limited...
October 21, 2016: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/27770875/nanostructured-screen-printed-electrodes-based-on-titanate-nanowires-for-biosensing-applications
#20
Helen Conceição Ferraz, Daniela Ferreira Machado, Neuman Solange de Resende
This work demonstrates the successful modification of screen-printed electrodes using functionalized titanate nanowires for producing a peroxide biosensor. Titanate nanowires were synthesized by the hydrothermal method and characterized using physico-chemical techniques. The surface of the nanowires was modified with (3-aminopropyl)trimethoxysilane and glutaraldehyde to immobilize horseradish peroxidase through covalent bound, obtaining a surface coverage of 1.62mg of enzyme/m(2). The surface of screen-printed carbon electrodes was modified with peroxidase-containing nanowires...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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