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https://www.readbyqxmd.com/read/28344154/characterization-of-toxoplasma-gondii-isolates-from-herds-of-sheep-in-southern-brazil-reveals-the-archetypal-type-ii-genotype-and-new-non-archetypal-genotypes
#1
Tatiana da Silva Ramos, Hilda Fátima de Jesus Pena, Alceu Gonçalves Dos Santos Junior, Laura Maria Jorge de Faria Santos, Beatris Gonzales Cademartori, Solange Oliveira, Solange Maria Gennari, Andréa da Silva Ramos Rocha, Nara Amélia da Rosa Farias
Recent studies have demonstrated that, in Brazil and South America, strains of Toxoplasma gondii are often genotypically and biologically different from those found in countries on other continents. The objective of this study was to genotypically characterize T. gondii isolates from naturally infected sheep in herds in the southern region of the state of Rio Grande do Sul, Brazil, by means of the polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP). Five T. gondii isolates obtained from sheep in five municipalities in the state of Rio Grande do Sul were used...
March 23, 2017: Parasitology International
https://www.readbyqxmd.com/read/28343393/selenium-bioaccessibility-and-speciation-in-selenium-enriched-lettuce-investigation-of-the-selenocompounds-liberated-after-in-vitro-simulated-human-digestion-using-two-dimensional-hplc-icp-ms
#2
Emanueli DO Nascimento DA Silva, Federica Aureli, Marilena D'Amato, Andrea Raggi, Solange Cadore, Francesco Cubadda
The evaluation of selenium-enriched vegetables as potential dietary sources of selenium, an essential element for humans, requires an assessment of the plant accumulation ability as well as of the bioaccessibility and speciation of the accumulated selenium, which influence its biological effects in humans. Lettuce hydroponically grown at three selenite (SeVI)/selenate (SeIV) amendment levels was characterized accordingly. Selenium accumulation in lettuce leaves was greatest with Se(VI) amendment, whereas bioaccessibility was 70% on average in both cases...
March 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28333295/drug-resistance-in-antiretroviral-naive-children-newly-diagnosed-with-hiv-1-in-manaus-amazonas
#3
Solange Dourado de Andrade, Meritxell Sabidó, Wuelton Marcelo Monteiro, Adele Schwartz Benzaken, Amilcar Tanuri
Objectives: To determine the prevalence of drug resistance mutations (DRM), the prevalence of drug susceptibility [transmitted drug resistance (TDR)] and the prevalence of HIV-1 variants among treatment-naive HIV-infected children in Manaus, Amazonas state, Brazil. Methods: Children born to HIV-infected mothers and diagnosed with HIV in an HIV reference service centre and with available pol sequence between 2010 and 2015 prior to antiretroviral initiation were included...
March 13, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28327803/serodiagnosis-of-visceral-and-cutaneous-leishmaniasis-in-human-and-canine-populations-living-in-indigenous-reserves-in-the-brazilian-amazon-region
#4
Julia Teresa Ribeiro de Lima, Solange Maria Gennari, Herbert Sousa Soares, Antonio Humberto Hamad Minervino, Antonio Francisco Malheiros, Fernando Silveira Marques, Márcia Dalastra Laurenti, Rosangela Zacarias Machado, Arlei Marcili, Marcelo Bahia Labruna, Rodrigo Martins Soares
INTRODUCTION: Leishmaniasis is endemic to the Northern, Northeastern, Central-Western, and Southeastern regions of Brazil. We aimed to assess the epidemiological situation of leishmaniasis in humans and dogs in indigenous villages located in the States of Mato Grosso and Tocantins using a serological survey conducted in May 2011. METHODS: Serum samples were collected from 470 humans and 327 dogs living in villages of the Urubu Branco and Tapirapé Karajá indigenous reserves...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28326851/evaluation-of-antibacterial-activity-of-the-bark-and-leaf-extracts-of-brosimum-gaudichaudii-tr%C3%A3-cul-against-multidrug-resistant-strains
#5
Joelma da Costa Borges, Michele Cezimbra Perim, Rodrigo Orlandini de Castro, Thiago Antônio de Sousa Araújo, Tadeu José da Silva Peixoto Sobrinho, Ana Carolina Oliveira da Silva, Sandra Maria Botelho Mariano, Solange Cristina Carreiro, Maria Cristina da Silva Pranchevicius
Brosimum gaudichaudii Trécul., a plant that belongs to Moraceae family, is found throughout the Brazilian Cerrado. The antimicrobial activities of ethanolic bark and leaf extracts of B. gaudichaudii were tested against multiresistant bacteria isolated from diabetic foot infections (DFIs). Antimicrobial activity of the extracts was evaluated by agar disc diffusion (DD) and broth dilution (BD) methods. By BD method, bark (53.85, 45.83%) and leaf (42.31, 50.00%) extracts contained antimicrobial activity against both gram-negative and gram-positive bacteria...
March 22, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28325675/placental-and-colostral-transfer-of-antibodies-reactive-with-enteropathogenic-escherichia-coli-intimins-%C3%AE-%C3%AE-or-%C3%AE
#6
Silvia P N Altman, Milene Tino-De-Franco, Cristiane B Carbonare, Patricia Palmeira, Solange B Carbonare
OBJECTIVE: Intimins are protein adhesins of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) capable of inducing attachment and effacement lesions in enterocytes. Anti-intimin antibodies are important for the protection from EPEC and EHEC infections because these antibodies inhibit bacterial adhesion and impair the initial step of the pathogenesis. We studied the transfer of maternal anti-intimin antibodies from healthy Brazilian mothers to their newborns through the placenta and colostrum...
March 19, 2017: Jornal de Pediatria
https://www.readbyqxmd.com/read/28318563/characterization-of-enterococcus-species-isolated-from-marine-recreational-waters-by-maldi-tof-ms-and-rapid-id-api%C3%A2-20-strep-system
#7
Ana Paula Guarnieri Christ, Solange Rodrigues Ramos, Rodrigo Cayô, Ana Cristina Gales, Elayse Maria Hachich, Maria Inês Zanoli Sato
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods...
March 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28315227/insulin-like-growth-factor-1-gene-polymorphism-in-women-with-breast-cancer
#8
Danylo Rafhael Costa-Silva, Maria da Conceição Barros-Oliveira, Rafael Soares Borges, Larysse Maira Campos-Verdes, João Paulo da Silva-Sampaio, Carla Solange Escorcio-Dourado, Luana Mota Martins, Airlane Pereira Alencar, Edmund Chada Baracat, Vladimir Costa Silva, Benedito Borges da Silva
Breast cancer is a disease of unknown etiology; however, the major risk factors are genetic alterations. Studies have demonstrated an association between insulin-like growth factor 1 (IGF-1) gene polymorphism and cell proliferation and reduced apoptosis, in addition to its role in breast cancer growth and aggressiveness. Two polymorphic variants of the IGF-1 gene are highlighted in association with breast cancer, rs6220 and rs7136446, although controversy exists as to this relationship. The current study included 137 women (68 breast cancer cases and 69 controls without breast cancer) who had 3 ml of peripheral blood drawn for the study of genomic DNA extracted from leukocytes using the genotyping technique by real-time polymerase chain reaction...
April 2017: Medical Oncology
https://www.readbyqxmd.com/read/28315039/ecology-and-feeding-habits-drive-infection-of-water-bugs-with-mycobacterium-ulcerans
#9
Solange Meyin A Ebong, Gabriel E García-Peña, Dominique Pluot-Sigwalt, Laurent Marsollier, Philippe Le Gall, Sara Eyangoh, Jean-François Guégan
Mycobacterium ulcerans (MU), the causative agent of Buruli ulcer, is present in a wide spectrum of environments, including terrestrial and aquatic ecosystems in tropical regions. The most promising studies on the epidemiological risk of this disease suggest that some ecological settings may favor infection of animals with MU including human. A species' needs and impacts on resources and the environment, i.e., its ecological niche, may influence its susceptibility to be infected by this microbial form. For example, some Naucoridae may dive in fresh waters to prey upon infected animals and thus may get infected with MU...
March 17, 2017: EcoHealth
https://www.readbyqxmd.com/read/28303497/n-acetylcysteine-prevents-alcohol-related-neuroinflammation-in-rats
#10
Ricardo Schneider, Solange Bandiera, Débora Guerini Souza, Bruna Bellaver, Greice Caletti, André Quincozes-Santos, Elaine Elisabetsky, Rosane Gomez
Alcoholism has been characterized as a systemic pro-inflammatory condition and alcohol withdrawal has been linked to various changes in the brain homeostasis, including oxidative stress and glutamate hyperactivity. N-acetylcysteine (NAC) is an anti-inflammatory and antioxidant multi-target drug with promising results in psychiatry, including drug addiction. We assessed the effects of NAC on the serum and brain inflammatory cytokines after cessation of chronic alcohol treatment in rats. Male Wistar rats received 2 g/kg alcohol or vehicle twice a day by oral gavage for 30 days...
March 16, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28296523/the-brazilian-amazon-region-eye-survey-design-and-methods
#11
Solange R Salomão, João Marcello Furtado, Adriana Berezovsky, Nívea N Cavascan, Alberto N Ferraz, Jacob M Cohen, Sergio Muñoz, Rubens Belfort
PURPOSE: To describe the study design, operational strategies, procedures, and baseline characteristics of the Brazilian Amazon Region Eye Survey (BARES), a population-based survey of the prevalence and causes of distance and near visual impairment and blindness in older adults residing in the city of Parintins. METHODS: Cluster sampling, based on geographically defined census sectors, was used for cross-sectional random sampling of persons 45 years and older from urban and rural areas...
March 15, 2017: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/28294484/interactive-audience-response-systems-in-oral-and-maxillofacial-radiology-undergraduate-lectures
#12
Christiano de Oliveira-Santos, Camila Tirapelli, Clarissa Teles Rodrigues, Carina Domaneschi, Solange Aparecida Caldeira Monteiro
OBJECTIVES: To evaluate the impact of audience response systems (ARS) on student participation (SP) during Oral and Maxillofacial Radiology (OMR) undergraduate lectures and on final examination scores (FES). Furthermore, an analysis of unanimity assessed the influence of ARS on students' responses. Students' perceptions were also assessed. METHODS: A controlled crossover study was designed. Four lectures covering topics of OMR were each taught with ARS and without ARS (i...
March 15, 2017: European Journal of Dental Education: Official Journal of the Association for Dental Education in Europe
https://www.readbyqxmd.com/read/28288226/-quality-scale-for-preschool-spirometry-interpretation
#13
Francisca Gatto, Paula Bedregal, Carlos Ubilla, Hortensia Barrientos, Solange Caussade
Since 2007, there are international guidelines for implementation and interpretation of spirometry in preschool children. A percentage of these patients cannot obtain maneuvers that meet all eligibility criteria. The objective of this study was to develop a quality scale for interpreting these partially acceptable spirometry. MATERIAL AND METHOD: Delphi methodology was used, which allows to reach consensus among experts analyzing a defined problem. We invited to participate pediatric pneumologists dedicated to lung function and who participated actively in scientific specialty societies in Chile...
February 2017: Revista Chilena de Pediatría
https://www.readbyqxmd.com/read/28287825/quantification-of-residual-germinal-center-activity-and-hiv-1-dna-and-rna-levels-using-fine-needle-biopsies-of-lymph-nodes-during-antiretroviral-therapy
#14
William J Hey-Nguyen, Yin Xu, Chester F Pearson, Michelle Bailey, Kazuo Suzuki, Robyn Tantau, Solange Obeid, Brad Milner, Andrew Field, Andrew Carr, Mark Bloch, David A Cooper, Anthony D Kelleher, John J Zaunders, Kersten K Koelsch
HIV-1 reservoirs are most often studied in peripheral blood (PB), but not all lymphocytes recirculate, particularly T follicular helper (Tfh) CD4(+) T cells, as well as germinal center (GC) B cells, in lymph nodes (LNs). Ultrasound-guided fine needle biopsies (FNBs) from inguinal LNs and PB samples were obtained from 10 healthy controls (HCs) and 21 HIV-1-infected subjects [11 antiretroviral therapy (ART) naive and 10 on ART]. Tfh cells and GC B cells were enumerated by flow cytometry. HIV-1 DNA and cell-associated (CA) RNA levels in LNs and PB were quantified by real-time polymerase chain reaction...
March 13, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28277426/effect-of-cold-water-immersion-performed-on-successive-days-on-physical-performance-muscle-damage-and-inflammatory-hormonal-and-oxidative-stress-markers-in-volleyball-players
#15
Victor H de Freitas, Solange P Ramos, Maurício G Bara-Filho, Daniel G S Freitas, Danilo R Coimbra, Rubens Cecchini, Flávia A Guarnier, Fábio Y Nakamura
The aim of this study was to investigate the effects of daily cold water immersion (CWI) on physical performance, muscle damage, and inflammatory, hormonal, and oxidative stress markers in volleyball. Six players were submitted to CWI and six players to a placebo, during 5 training days. Thigh circumference, squat jump, and agility were measured on the 1, 3 and 6 days. On the 1 and 6 days, blood and saliva were collected for analysis of oxidative stress, muscle damage, and inflammatory and hormonal levels. Muscle soreness and countermovement jump were quantified daily...
March 8, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28274494/prevalence-of-fibromyalgia-in-patients-treated-at-the-bariatric-surgery-outpatient-clinic-of-hospital-de-cl%C3%A3-nicas-do-paran%C3%A3-curitiba
#16
Deborah Negrão Gonçalo Dias, Márcia Alessandra Arantes Marques, Solange C Bettini, Eduardo Dos Santos Paiva
INTRODUCTION: Fibromyalgia (FM) is a chronic pain syndrome characterized by generalized pain. It is known that obese patients have more skeletal muscle pain and physical dysfunction than normal weight patients. Therefore, it is important that the early diagnosis of FM be attained in obese patients. OBJECTIVE: To determine the prevalence of FM in a group of obese patients with indication of bariatric surgery. MATERIALS AND METHODS: The patients were recruited from the Bariatric Surgery outpatient clinic of Hospital de Clínicas of UFPR (HC-UFPR) before being submitted to surgery...
February 20, 2017: Revista Brasileira de Reumatologia
https://www.readbyqxmd.com/read/28270305/carboxymethyl-cellulose-trichloroacetic-acid-and-unna-boot-for-healing-venous-ulcers-a-comparative-study-of-their-efficacy-and-socioeconomic-impact
#17
Sueli Carneiro, Virginia Januário, Maria Leide W Oliveira, Maria Luiza de Oliveira Teixeira, Solange Maciel, João Carlos Macedo, Marcia Ramos-E-Silva
Chronic ulcers are defined as a breakdown of the long-term cutaneous barrier or frequent recurrence of breakdowns. Dressings are a form of treatment and, in view of the variety and high cost of the products on the market, three agents were selected for this investigation, regarding their efficacy and cost/benefit ratio. The objective of the investigation was to evaluate and compare the efficacy, costs, and benefits of carboxymethylcellulose in paste at 20% (CMC 20%), trichloroacetic acid at 90% (TCA 90%), and Unna boot for treatment of chronic venous ulcers...
2017: Skinmed
https://www.readbyqxmd.com/read/28265064/multisensor-integrated-organs-on-chips-platform-for-automated-and-continual-in-situ-monitoring-of-organoid-behaviors
#18
Yu Shrike Zhang, Julio Aleman, Su Ryon Shin, Tugba Kilic, Duckjin Kim, Seyed Ali Mousavi Shaegh, Solange Massa, Reza Riahi, Sukyoung Chae, Ning Hu, Huseyin Avci, Weijia Zhang, Antonia Silvestri, Amir Sanati Nezhad, Ahmad Manbohi, Fabio De Ferrari, Alessandro Polini, Giovanni Calzone, Noor Shaikh, Parissa Alerasool, Erica Budina, Jian Kang, Nupura Bhise, João Ribas, Adel Pourmand, Aleksander Skardal, Thomas Shupe, Colin E Bishop, Mehmet Remzi Dokmeci, Anthony Atala, Ali Khademhosseini
Organ-on-a-chip systems are miniaturized microfluidic 3D human tissue and organ models designed to recapitulate the important biological and physiological parameters of their in vivo counterparts. They have recently emerged as a viable platform for personalized medicine and drug screening. These in vitro models, featuring biomimetic compositions, architectures, and functions, are expected to replace the conventional planar, static cell cultures and bridge the gap between the currently used preclinical animal models and the human body...
March 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28252282/reverse-microemulsion-based-synthesis-of-bis-phosphonate-metal-materials-with-controllable-physical-properties-an-example-using-zoledronic-acid-calcium-complexes
#19
Xu Li, Youssef W Naguib, Solange Valdes, Stephanie Hufnagel, Zhengrong Cui
The development of phosphonate-metal materials is tightly related to the advancement in their synthesis methods. Herein, using zoledronic acid (Zol), a bisphosphonate (bioacitve phosphonate with a 'P-C-P' structure), and calcium as model molecules, we applied the reverse microemulsion (RM) to synthesize a series of Zol-Ca complexes. We comprehensively i) studied the relationship between RM conditions, including the component ratio of RM, co-surfactants, reaction time, reactant concentrations, reaction temperature, and the presence of a phospholipid, 1, 2-dioleoyl-sn-glycero-3-phosphate acid (DOPA), and the physical properties of the complexes synthesized (i...
March 2, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28250962/the-herts-and-minds-study-evaluating-the-effectiveness-of-mentalization-based-treatment-mbt-as-an-intervention-for-children-in-foster-care-with-emotional-and-or-behavioural-problems-a-phase-ii-feasibility-randomised-controlled-trial
#20
Nick Midgley, Sarah Jane Besser, Helen Dye, Pasco Fearon, Tim Gale, Kiri Jefferies-Sewell, Karen Irvine, Joyce Robinson, Solange Wyatt, David Wellsted, Sally Wood
BACKGROUND: A significant proportion of children in the social care system in England present with mental health problems, with the majority experiencing some form of emotional and behavioural difficulties. The most effective treatments for these children are currently unknown, partly due to a lack of robust, controlled studies. Researchers have identified a number of obstacles to conducting well-designed research with this population, making the need to test the feasibility of a randomised controlled trial especially important...
2017: Pilot and Feasibility Studies
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