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https://www.readbyqxmd.com/read/29454191/show-me-the-way-rust-effector-targets-in-heterologous-plant-systems
#1
REVIEW
Cécile Lorrain, Benjamin Petre, Sébastien Duplessis
For years, the study of rust fungal effectors has been impeded by the lack of molecular genetic tools in rust pathosystems. The recent use of heterologous plants to perform effector screens (effectoromics)-including effector localisation (cellular targets) and protein interactors (molecular targets) in plant cells-has changed the game. These screens revealed that many candidate effectors from various rust fungi target specific plant cell compartments, including chloroplasts, and associate with specific plant protein complexes...
February 14, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29454060/aop-db-a-database-resource-for-the-exploration-of-adverse-outcome-pathways-through-integrated-association-networks
#2
Maureen E Pittman, Stephen W Edwards, Cataia Ives, Holly M Mortensen
The Adverse Outcome Pathway (AOP) framework describes the progression of a toxicity pathway from molecular perturbation to population-level outcome in a series of measurable, mechanistic responses. The controlled, computer-readable vocabulary that defines an AOP has the ability to, automatically and on a large scale, integrate AOP knowledge with publically available sources of biological high-throughput data and its derived associations. To support the discovery and development of putative (existing) and potential AOPs, we introduce the AOP-DB, an exploratory database resource that aggregates association relationships between genes and their related chemicals, diseases, pathways, species orthology information, ontologies, and gene interactions...
February 14, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29453673/optical-coherence-tomography-angiography-discerns-preclinical-diabetic-retinopathy-in-eyes-of-patients-with-type-2-diabetes-without-clinical-diabetic-retinopathy
#3
Dan Cao, Dawei Yang, Zhongning Huang, Yunkao Zeng, Jun Wang, Yunyan Hu, Liang Zhang
AIMS: To investigate changes in retinal vascular plexuses and choriocapillaris in patients with type 2 diabetes mellitus (DM2) without diabetic retinopathy (DR) and healthy controls using optical coherence tomography angiography (OCTA). METHODS: A total of 71 DM2 and 67 healthy control subjects were included. All subjects underwent OCTA examination (RTVue-XR Avanti; Optovue, Fremont, CA, USA). Average vessel density in superficial capillary plexus (SCP), deep capillary plexus (DCP) and choriocapillaris, parafoveal vessel density in SCP and DCP, FAZ area (mm 2 ) in SCP, microaneurysms and capillary nonperfusion were taken into analysis...
February 16, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29453636/assessment-of-oximetry-based-statistical-classifiers-as-simplified-screening-tools-in-the-management-of-childhood-obstructive-sleep-apnea
#4
Andrea Crespo, Daniel Álvarez, Leila Kheirandish-Gozal, Gonzalo C Gutiérrez-Tobal, Ana Cerezo-Hernández, David Gozal, Roberto Hornero, Félix Del Campo
PURPOSE: A variety of statistical models based on overnight oximetry has been proposed to simplify the detection of children with suspected obstructive sleep apnea syndrome (OSAS). Despite the usefulness reported, additional thorough comparative analyses are required. This study was aimed at assessing common binary classification models from oximetry for the detection of childhood OSAS. METHODS: Overnight oximetry recordings from 176 children referred for clinical suspicion of OSAS were acquired during in-lab polysomnography...
February 16, 2018: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29453370/a-multitarget-approach-to-drug-discovery-inhibiting-mycobacterium-tuberculosis-pyrg-and-pank
#5
Laurent R Chiarelli, Giorgia Mori, Beatrice Silvia Orena, Marta Esposito, Thomas Lane, Ana Luisa de Jesus Lopes Ribeiro, Giulia Degiacomi, Júlia Zemanová, Sára Szádocka, Stanislav Huszár, Zuzana Palčeková, Marcello Manfredi, Fabio Gosetti, Joël Lelièvre, Lluis Ballell, Elena Kazakova, Vadim Makarov, Emilio Marengo, Katarina Mikusova, Stewart T Cole, Giovanna Riccardi, Sean Ekins, Maria Rosalia Pasca
Mycobacterium tuberculosis, the etiological agent of the infectious disease tuberculosis, kills approximately 1.5 million people annually, while the spread of multidrug-resistant strains is of great global concern. Thus, continuous efforts to identify new antitubercular drugs as well as novel targets are crucial. Recently, two prodrugs activated by the monooxygenase EthA, 7947882 and 7904688, which target the CTP synthetase PyrG, were identified and characterized. In this work, microbiological, biochemical, and in silico methodologies were used to demonstrate that both prodrugs possess a second target, the pantothenate kinase PanK...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453257/a-facile-arsenazo-iii-based-assay-for-monitoring-rare-earth-element-depletion-from-cultivation-media-of-methanotrophic-and-methylotrophic-bacteria
#6
Carmen Hogendoorn, Paula Roszczenko-Jasińska, N Cecilia Martinez-Gomez, Johann de Graaff, Patrick Grassl, Arjan Pol, Huub J M Op den Camp, Lena J Daumann
Recently, methanotrophic and methylotrophic bacteria were found to utilize rare earth elements (REE). To monitor the REE-content in culture media of these bacteria we have developed a rapid screening method using the Arsenazo III (AS III) dye for spectrophotometric REE-detection in the low μM (0.1-10 μM) range. We designed this assay to follow La III and Eu III depletion from the culture medium by the acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV. The assay can also be modified to screen the uptake of other REE such as Pr III or to monitor the depletion of La III from growth media in neutrophilic methylotrophs such as Methylobacterium extorquens AM1...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29453190/testing-behavior-change-techniques-to-encourage-primary-care-physicians-to-access-cancer-screening-audit-and-feedback-reports-protocol-for-a-factorial-randomized-experiment-of-email-content
#7
Gratianne Vaisson, Holly O Witteman, Zachary Bouck, Caroline A Bravo, Laura Desveaux, Diego Llovet, Justin Presseau, Marianne Saragosa, Monica Taljaard, Shama Umar, Jeremy M Grimshaw, Jill Tinmouth, Noah M Ivers
BACKGROUND: Cancer Care Ontario's Screening Activity Report (SAR) is an online audit and feedback tool designed to help primary care physicians in Ontario, Canada, identify patients who are overdue for cancer screening or have abnormal results requiring follow-up. Use of the SAR is associated with increased screening rates. To encourage SAR use, Cancer Care Ontario sends monthly emails to registered primary care physicians announcing that updated data are available. However, analytics reveal that 50% of email recipients do not open the email and less than 7% click the embedded link to log in to their report...
February 16, 2018: JMIR Research Protocols
https://www.readbyqxmd.com/read/29453188/web-based-alcohol-smoking-and-substance-involvement-screening-test-results-for-the-general-spanish-population-cross-sectional-study
#8
Juan A Lopez-Rodriguez, Gabriel Rubio Valladolid
BACKGROUND: Information technology in health sciences could be a screening tool of great potential and has been shown to be effective in identifying single-drug users at risk. Although there are many published tests for single-drug screening, there is a gap for concomitant drug use screening in general population. The ASSIST (Alcohol, Smoking and Substance Involvement Screening Test) website was launched on February 2015 in Madrid, Spain, as a tool to identify those at risk. OBJECTIVE: The aim of this study was to describe the use of a tool and to analyze profiles of drug users, their consumption patterns, and associated factors...
February 16, 2018: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/29453154/unique-pharmacology-of-a-novel-allosteric-agonist-sensitizer-insulin-receptor-monoclonal-antibody
#9
Simon A Hinke, Anne M Cieniewicz, Thomas Kirchner, Katharine D'Aquino, Rupesh Nanjunda, Jason Aligo, Robert Perkinson, Philip Cooper, Ken Boayke, Mark L Chiu, Steve Jarantow, Eilyn R Lacy, Yin Liang, Dana L Johnson, Jean M Whaley, Russell B Lingham, Anthony J Kihm
OBJECTIVE: Insulin resistance is a key feature of Type 2 Diabetes (T2D), and improving insulin sensitivity is important for disease management. Allosteric modulation of the insulin receptor (IR) with monoclonal antibodies (mAbs) can enhance insulin sensitivity and restore glycemic control in animal models of T2D. METHODS: A novel human mAb, IRAB-A, was identified by phage screening using competition binding and surface plasmon resonance assays with the IR extracellular domain...
February 3, 2018: Molecular Metabolism
https://www.readbyqxmd.com/read/29452777/yoga-for-menopausal-symptoms-a-systematic-review-and-meta-analysis
#10
REVIEW
Holger Cramer, Wenbo Peng, Romy Lauche
OBJECTIVES: To systematically review and meta-analyze the effectiveness of yoga for menopausal symptoms. METHODS: Medline (via PubMed), the Cochrane Central Register of Controlled Trials, and Scopus were screened through to February 21, 2017 for randomized controlled trials (RCTs) comparing the effects of yoga on menopausal symptoms to those of no treatment or active comparators. Standardized mean differences (SMD) and 95% confidence intervals (CI) were calculated...
March 2018: Maturitas
https://www.readbyqxmd.com/read/29452686/technological-advances-and-changing-indications-for-lumbar-puncture-in-neurological-disorders
#11
REVIEW
Joost M Costerus, Matthijs C Brouwer, Diederik van de Beek
Technological advances have changed the indications for and the way in which lumbar puncture is done. Suspected CNS infection remains the most common indication for lumbar puncture, but new molecular techniques have broadened CSF analysis indications, such as the determination of neuronal autoantibodies in autoimmune encephalitis. New screening techniques have increased sensitvity for pathogen detection and can be used to identify pathogens that were previously unknown to cause CNS infections. Evidence suggests that potential treatments for neurodegenerative diseases, such as Alzheimer's disease, will rely on early detection of the disease with the use of CSF biomarkers...
March 2018: Lancet Neurology
https://www.readbyqxmd.com/read/29452550/an-eye-on-vision-20-questions-about-vision-screening-and-eye-health
#12
P Kay Nottingham Chaplin, Kira Baldonado, Geoffrey E Bradford, Susan Cotter, Bruce Moore
Current evidence-based and best practice vision screening and eye health approaches, tools, and procedures are the result of revised national guidelines in the past 3 years and advances in research during the last 16 years. To help the busy school nurse with little time to keep up with changes in children's vision practices and a growing body of literature, the National Center for Children's Vision and Eye Health at Prevent Blindness is providing answers to 20 questions received most often from the field. Question topics are: (1) arranging the screening environment, (2) occluders to cover the eyes during vision screening, (3) optotype-based screening at distance, (4) optotype-based screening at near, (5) instrument-based screening, (6) muscle imbalance screening, (7) referrals, and (8) vision screening certification...
March 2018: NASN School Nurse
https://www.readbyqxmd.com/read/29452533/serum-ca125-in-combination-with-ferritin-improves-diagnostic-accuracy-for-epithelial-ovarian-cancer
#13
J Zhao, N Guo, L Zhang, L Wang
INTRODUCTION: CA125 has poor sensitivity and low specificity for detecting early ovarian cancer. Serum ferritin is elevated in many malignancies. We evaluated the performance of ferritin alone and in combination with CA125 as a diagnostic tool to detect epithelial ovarian cancer (EOC). METHODS: CA125 and ferritin were detected in the serum of 50 healthy control (HC), 50 women with benign gynaecological conditions and 124 women with EOC. The relationship between serum ferritin and CA125 and each of the clinicopathological parameters was assessed, and their diagnostic accuracy for discriminating ovarian cancer determined...
February 16, 2018: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/29452343/height-based-equations-can-improve-the-diagnosis-of-elevated-blood-pressure-in-children
#14
F A Mourato, S S Mattos, J L Lima Filho, M F Mourato, W Nadruz JÚnior
Background: High blood pressure (BP) is usually underdiagnosed in children and adolescents, particularly due to its complex diagnosis process. This study describes novel height-based equations for the detection of BP disorders (BP>90th percentile) and compares the accuracy of this approach with previously described screening methods to identify BP disorders. Methods: Height-based equations were built using the 90th percentile values for systolic and diastolic BP and respective height values from the current guideline of high BP management in children...
February 14, 2018: American Journal of Hypertension
https://www.readbyqxmd.com/read/29451720/detection-of-membrane-protein-protein-interaction-in-planta-based-on-dual-intein-coupled-tripartite-split-gfp-association
#15
Tzu-Yin Liu, Wen-Chun Chou, Wei-Yuan Chen, Ching-Yi Chu, Chen-Yi Dai, Pei-Yu Wu
Despite a great interest of identification of protein-protein interactions (PPIs) in biological systems, only few attempts have been made for a large-scale PPI screen in planta. Unlike biochemical assays, bimolecular fluorescence complementation (BiFC) allows visualization of the transient and weak PPIs in vivo at the subcellular resolution. However, when the non-fluorescent fragments are highly expressed, the spontaneous and irreversible self-assembly of the split halves can easily generate false positives...
February 16, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29451114/molecular-tools-to-study-preharvest-food-safety-challenges
#16
Deepak Kumar, Siddhartha Thakur
Preharvest food safety research and activities have advanced over time with the recognition of the importance and complicated nature of the preharvest phase of food production. In developed nations, implementation of preharvest food safety procedures along with strict monitoring and containment at various postharvest stages such as slaughter, processing, storage, and distribution have remarkably reduced the burden of foodborne pathogens in humans. Early detection and adequate surveillance of pathogens at the preharvest stage is of the utmost importance to ensure a safe meat supply...
February 2018: Microbiology Spectrum
https://www.readbyqxmd.com/read/29450702/establishment-and-characterization-of-the-ncc-ss1-c1-synovial-sarcoma-cell-line
#17
Fusako Kito, Rieko Oyama, Yoko Takai, Marimu Sakumoto, Kumiko Shiozawa, Zhiwei Qiao, Takenori Uehara, Akihiko Yoshida, Akira Kawai, Tadashi Kondo
Synovial sarcoma is an aggressive mesenchymal malignancy characterized by unique gene fusions. Tissue culture cells are essential tools for further understanding tumorigenesis and anti-cancer drug development; however, only a limited number of well-characterized synovial sarcoma cell lines exist. Thus, the objective of this study was to establish a patient-derived synovial sarcoma cell line. We established a synovial sarcoma cell line from tumor tissue isolated from a 72-year-old female patient. Prepared cells were analyzed for the presence of gene fusions by fluorescence in situ hybridization, RT-PCR, and karyotyping...
February 15, 2018: Human Cell
https://www.readbyqxmd.com/read/29450667/a-risk-prediction-model-to-allow-personalized-screening-for-cervical-cancer
#18
Michael B Rothberg, Bo Hu, Laura Lipold, Sarah Schramm, Xian Wen Jin, Andrea Sikon, Glen B Taksler
IMPORTANCE: Cervical cancer screening guidelines are in evolution. Current guidelines do not differentiate recommendations based on individual patient risk. OBJECTIVE: To derive and validate a tool for predicting individualized probability of cervical intraepithelial neoplasia grade 2 or higher (CIN2+) at a single time point, based on demographic factors and medical history. DESIGN: The study design consisted of an observational cohort with hierarchical generalized linear regression modeling...
February 15, 2018: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/29450235/vietnamese-validation-of-the-short-version-of-internet-addiction-test
#19
Bach Xuan Tran, Hue Thi Mai, Long Hoang Nguyen, Cuong Tat Nguyen, Carl A Latkin, Melvyn W B Zhang, Roger C M Ho
Background and aims: The main goal of the present study was to examine the psychometric properties of a Vietnamese version of the short-version of Internet Addiction Test (s-IAT) and to assess the relationship between s-IAT scores and demographics, health related qualify of life and perceived stress scores in young Vietnamese. Methods: The Vietnamese version of s-IAT was administered to a sample of 589 participants. Exploratory factor and reliability analyses were performed...
December 2017: Addictive Behaviors Reports
https://www.readbyqxmd.com/read/29450149/human-and-mouse-microarrays-guided-expression-analysis-of-membrane-protein-trafficking-related-genes-in-mdck-cells-a-canine-epithelial-model-for-apical-and-basolateral-differential-protein-targeting
#20
Xiaofan Xu, Mingming Pan, Alexis E Gasiewicz, Rongzi Li, Shiu-Ming Kuo
MDCK cells are widely used to study the differential targeting of membrane transporters to apical and basolateral membrane but its canine origin limited the commercial tools available for the analysis of protein trafficking machinery. Because apical and basolateral membranes are only found in differentiated epithelial cells, genes critical for differential targeting may be specifically up-regulated upon MDCK cell differentiation. To search for these genes, a cross-species screening strategy was used. We first analyzed the human microarray data for protein trafficking-related genes that were up-regulated in colon carcinoma Caco2 cells upon differentiation...
June 2017: Biochimie Open
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