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Tropomyosin

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https://www.readbyqxmd.com/read/29329856/effect-of-tyrosinase-aided-crosslinking-on-the-ige-binding-potential-and-conformational-structure-of-shrimp-metapenaeus-ensis-tropomyosin
#1
Ishfaq Ahmed, Liangtao Lv, Hong Lin, Zhenxing Li, Jiaju Ma, Chen Guanzhi, Lirui Sun, Lili Xu
The present study was performed to determine crosslinking and oxidative reactions catalyzed by tyrosinase (Tyr), caffeic acid (CA) and their combination with respect to IgE binding potential and conformational structure of shrimp tropomyosin (TM). Cross-links and IgE binding potentials were analyzed by SDS-PAGE, western blot and indirect ELISA. While structural changes were characterized using surface hydrophobicity, ultraviolet (UV), fluorescence and circular dichroism (CD) spectroscopies. Maximum reduction in the IgG (37...
May 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29322793/mir-1-3p-inhibits-cell-proliferation-and-invasion-by-regulating-bdnf-trkb-signaling-pathway-in-bladder-cancer
#2
L Gao, P Yan, F F Guo, H J Liu, Z F Zhao
Recent studies have confirmed the existence of BDNF and tropomyosin-related kinase B (TrkB) in normal and cancerous urothelium. However, the corresponding mechanisms and upstream signal pathways of BDNF/TrkB have not been fully discovered. This study aimed to investigate the effects of miR-1-3p on bladder cancer (BC) by regulating BDNF-TrkB signal pathway. The expression of miR-1-3p and BDNF in BC tissues and cell lines were detected by Cancer Genome Atlas (TCGA) microarray analysis, RT-qPCR and western blot...
2018: Neoplasma
https://www.readbyqxmd.com/read/29317496/a-functional-link-between-nad-homeostasis-and-n-terminal-protein-acetylation-in-saccharomyces-cerevisiae
#3
Trevor Croft, Christol James Theoga Raj, Michelle Salemi, Brett S Phinney, Su-Ju Lin
Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite participating in cellular redox chemistry and signaling, and the complex regulation of NAD+ metabolism is not yet fully understood. To investigate this, we established a NAD+-intermediate specific reporter system to identify factors required for salvage of metabolically-linked nicotinamide (NAM) and nicotinic acid (NA). Mutants lacking components of the NatB complex, NAT3 and MDM20, appeared as hits in this screen. NatB is an Nα-terminal-acetyltransferase responsible for acetylation of the amino terminus of specific Met-retained peptides...
January 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29315742/depression-and-anxiety-like-behaviour-is-related-to-bdnf-trkb-signalling-in-a-mouse-model-of-psoriasis
#4
W JiaWen, S Hong, X ShengXiang, L Jing
BACKGROUND: The prevalence of anxiety and depression is significantly higher in individuals with psoriasis than in the general population. Clinical data also show that anti-anxiety and antidepression drugs can reduce skin lesions in patients with psoriasis, but the actual mechanism is still poorly understood. AIM: To investigate whether brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrKB) signalling plays a role in the mechanism underlying psoriasis with depression and anxiety behaviours...
January 8, 2018: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/29312563/trkb-promotes-laryngeal-cancer-metastasis-via-activation-pi3k-akt-pathway
#5
Liang Jiang, Zhihai Wang, Chuan Liu, Zhitao Gong, Yucheng Yang, Houyong Kang, Yanshi Li, Guohua Hu
Objectives: The aim of our study was to investigate the role of TrkB pathway in tumor occurrence and development for in order to provide theoretical basis to laryngeal cancer therapy. Materials and methods: Biological characteristics of the cells were studied by migration tests and colony forming assay. Gene and protein expression analysis was performed by RT-PCR or western blot. in vivo experiments were conducted in syngeneic BALB/c mice. Results: Significant changes in protein and gene expression, including higher expression level of TrkB, were found in cells and laryngeal cancer specimens...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29311641/blockade-of-soluble-epoxide-hydrolase-attenuates-post-ischemic-neuronal-hyperexcitation-and-confers-resilience-against-stroke-with-trkb-activation
#6
Li-Hsin Chang, Hui-Ching Lin, Shiang-Suo Huang, I-Chih Chen, Kai-Wen Chu, Chun-Lien Chih, Yao-Wen Liang, Yi-Chung Lee, You-Yin Chen, Yi-Hsuan Lee, I-Hui Lee
Inhibition and deletion of soluble epoxide hydrolase (sEH) has been suggested to ameliorate infarction in experimental ischemic stroke possibly via vasoactive epoxyeicosatrienoic acids. However, it is unknown whether the neuroprotective mechanisms involve alteration of post-ischemic neuronal transmission and neurotrophic signaling. We used a permanent middle cerebral artery occlusion (MCAO) model in adult wild-type mice with the sEH inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA) post-treatment and in sEH knockout (sEH KO) mice...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29310803/smoothelins-and-the-control-of-muscle-contractility
#7
Megha Murali, Justin A MacDonald
Smooth muscle cells display distinctive expression and organization of contractile filament proteins, which reflect a unique method of contractile regulation. As the focus of this review, the smoothelin and smoothelin-like family members represent a family of poorly understood muscle proteins that appear to act as structural components of the contractile apparatus. The protein family is characterized by the presence a single C-terminal type-2 calponin homology (CH) domain. Often used as the preferred marker of differentiated contractile smooth muscle cells, smoothelin A and B (SMTN-A and SMTN-B) may influence the contractile potential of smooth muscle cells...
2018: Advances in Pharmacology
https://www.readbyqxmd.com/read/29305654/spontaneous-regression-of-neuroblastoma
#8
REVIEW
Garrett M Brodeur
Neuroblastomas are characterized by heterogeneous clinical behavior, from spontaneous regression or differentiation into a benign ganglioneuroma, to relentless progression despite aggressive, multimodality therapy. Indeed, neuroblastoma is unique among human cancers in terms of its propensity to undergo spontaneous regression. The strongest evidence for this comes from the mass screening studies conducted in Japan, North America and Europe and it is most evident in infants with stage 4S disease. This propensity is associated with a pattern of genomic change characterized by whole chromosome gains rather than segmental chromosome changes but the mechanism(s) underlying spontaneous regression are currently a matter of speculation...
January 5, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29298707/shuang-huang-lian-prevents-basophilic-granulocyte-activation-to-suppress-th2-immunity
#9
Qiaoling Fei, Yixin Han, Ruijuan Qi, Yuan Gao, Lei Fang, Rui Hou, Runlan Cai, Yun Qi
BACKGROUND: Basophilic granulocytes (BGs) not only initiate the induction of Th2 cell differentiation, but also amplify the ongoing Th2 response. Shuang-Huang-Lian (SHL) is clinically used for relieving type I hypersensitivity by continuous treatment for several weeks. METHODS: ELISA, flow cytometry, magnetic activated cell sorting, isoelectric precipitation, hybridoma technique, transfection and luciferase reporter assay were used in this study. The statistical analysis was performed using a one-way ANOVA...
January 3, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29287471/conformation-stability-in-vitro-digestibility-and-allergenicity-of-tropomyosin-from-shrimp-exopalaemon-modestus-as-affected-by-high-intensity-ultrasound
#10
Ziye Zhang, Xiaofeng Zhang, Wei Chen, Peng Zhou
The effects of high intensity ultrasound (HIU) modification (power: 100-800 W, 15 min) on the conformation stability, in vitro digestibility and allergenicity of tropomyosin (TM) from Exopalaemon modestus were investigated. HIU treatment oxidized the Cys, Met and Lys in TM and degraded TM to generate protein fragments by HIU-induced free radicals. The α-helix content decreased, while β-sheet, β-turn and random coil increased. The immunoblotting and ELISA data suggested HIU treatment reduced the allergenicity and contributed to higher digestibility of TM...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29287365/dephosphorylation-enhances-postmortem-degradation-of-myofibrillar-proteins
#11
Zheng Li, Meng Li, Manting Du, Qingwu W Shen, Dequan Zhang
Protein degradation is primarily responsible for postmortem meat tenderization, which might be affected by phosphorylation. The objective of this study was to investigate the effect of phosphorylation on myofibrillar proteins degradation in muscle during postmortem. Here we modulated the phosphorylation status of protein by protein kinase inhibitor and phosphatase inhibitor, and the effect of these inhibitors on myofibrillar protein degradation was evaluated. Generally, myofibril fragmentation index of samples with lower phosphorylation level was higher...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29287337/purification-and-characterization-of-a-protease-from-the-visceral-mass-of-mytella-charruana-and-its-evaluation-to-obtain-antimicrobial-peptides
#12
Leonardo Prezzi Dornelles, Maria de Fátima Deodato de Souza, Pollyanna Michelle da Silva, Thamara Figueiredo Procópio, Ricardo Salas Roldan Filho, Thâmarah de Albuquerque Lima, Ana Patrícia Silva de Oliveira, Russolina Benedeta Zingali, Patrícia Maria Guedes Paiva, Emmanuel Viana Pontual, Thiago Henrique Napoleão
This work describes purification of a protease from the visceral mass of the mussel Mytella charruana as well as evaluation of its ability to hydrolyze milk casein to generate antimicrobial peptides. The enzyme showed pI of 4.1 and a single polypeptide band of 83.1 kDa after SDS-PAGE. Sequence similarities with tropomyosin and myosin from mollusks were detected. The protease showed a trypsin-like activity with optimal temperature of 40 °C and stability in a wide pH range (3.0-9.0). Km was 4.28 ± 0...
April 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29281262/binding-of-zn-ii-to-tropomyosin-receptor-kinase-a-in-complex-with-its-cognate-nerve-growth-factor-insights-from-molecular-simulation-and-in-vitro-essays
#13
Adriana Pietropaolo, Antonio Magrì, Valentina Greco, Valeria Losasso, Diego La Mendola, Sebastiano Sciuto, Paolo Carloni, Enrico Rizzarelli
The binding of the human nerve growth factor (NGF) protein to the tropomyosin-receptor-kinase A (TrkA) is associated with Alzhemeir's development. Owing to the large presence of zinc(II) ions in the synaptic compartments, the zinc ions might be bound to the complex in vivo. Here, we have identified a putative zinc binding site using a combination of computations and experiments. First, we have predicted structural features of the NGF/TrkA complex in aqueous solution by molecular simulation. Metadynamics free energy calculations suggest that these are very similar to those in the X-ray structure...
December 27, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/29278785/gender-related-increase-of-tropomyosin-1-abundance-in-platelets-of-alzheimer-s-disease-and-mild-cognitive-impairment-patients
#14
Christina Maria Reumiller, Georg Johannes Schmidt, Ina Dhrami, Ellen Umlauf, Eduard Rappold, Maria Zellner
The incidence of Alzheimer's disease (AD) is higher in elderly women than in men. The molecular background for this gender-related risk, however, is largely unknown. In a previous proteomics study we identified significantly elevated levels of monoamine oxidase-B and tropomyosin-1 in AD patients together with significant changes of the genetic AD risk factors apolipoprotein E4 (APOE4) and glutathione S-transferase omega 1 (GSTO1) in platelets - a promising source for AD blood biomarkers. The present study aimed to investigate the gender-specificity as well as disease-stage dependency of these biomarkers in AD patients and those with mild cognitive impairment (MCI)...
December 23, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29278453/functional-role-of-the-core-gap-in-the-middle-part-of-tropomyosin
#15
Alexander M Matyushenko, Daniil V Shchepkin, Galina V Kopylova, Sergey Y Bershitsky, Natalia A Koubassova, Andrey K Tsaturyan, Dmitrii I Levitsky
Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein playing an essential role in the regulation of muscle contraction. The middle part of the Tpm molecule has some specific features, such as the presence of non-canonical residues as well as a substantial gap at the interhelical interface, which are believed to destabilize coiled-coil and impart structural flexibility to this part of the molecule. To study how the gap affects structural and functional properties of α-striated Tpm (Tpm1.1 isoform that is expressed in cardiac and skeletal muscles) we substituted large conserved apolar core residues located at both sides of the gap with smaller ones by mutations M127A/I130A and M141A/Q144A...
December 26, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29247376/neurotrophin-expression-in-lymphocytes-a-powerful-indicator-of-degeneration-in-parkinson-s-disease-amyotrophic-lateral-sclerosis-and-ataxia
#16
Anjana Sadanand, Anjali Janardhanan, A J Vanisree, Thamil Pavai
Deregulated neurotrophin is an etiological factor in the pathology of neurodegenerative diseases (ND) that are clinically different entities but characterised by similar limb dysfunction. Earlier validation of peripheral biomarkers can provide significant translational benefit to ND patients. We analysed brain-derived neurotrophic factor (BDNF)-tropomyosin possessing tyrosine-related kinase (Trk B) and its key downstream proteins which are implicated in ND such as Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) and ataxia...
December 16, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29245910/the-prognostic-value-of-over-expressed-trkb-in-solid-tumors-a-systematic-review-and-meta-analysis
#17
Chunze Zhang, Xiaoting Li, Dan Gao, Haihua Ruan, Zhenzhen Lin, Xiaobo Li, Guang Liu, Zhicheng Ma, Xichuan Li
It is reported recently Tropomyosin-related receptor Kinase B (TrkB) plays key roles in the anoikis resistance during the processes of tumorigenesis and metastasis. However, its prognostic significance for clinical patients remains inconclusive. In order to establish a correct and practicable link between increased TrkB and prognostication of human solid tumors, a meta-analysis was performed in this article. A systematic literature research in the electronic databases PubMed, Embase and Web of Science was performed to identify eligible studies...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29237803/anti-tumor-activity-of-entrectinib-a-pan-trk-ros1-and-alk-inhibitor-in-etv6-ntrk3-positive-acute-myeloid-leukemia
#18
Kristen M Smith, Patrick C Fagan, Elena Pomari, Giuseppe Germano, Chiara Frasson, Colin Walsh, Ian M Silverman, Paolo Bonvini, Gang Li
Activation of tropomyosin receptor kinase (TRK) family tyrosine kinases by chromosomal rearrangement has been shown to drive a wide range of solid tumors and hematologic malignancies. TRK fusions are actionable targets as evidenced by recent clinical trial results in solid tumors. Entrectinib (RXDX-101) is an investigational, orally available, CNS-active, highly potent and selective kinase inhibitor against TRKA/B/C, ROS1 and ALK kinase activities. Here, we demonstrate that TRK kinase inhibition by entrectinib selectively targets preclinical models of TRK fusion-driven hematologic malignancies...
December 13, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29237268/sesquiterpenoids-from-the-root-of-panax-ginseng-attenuates-lipopolysaccharide-induced-depressive-like-behavior-through-the-bdnf-trkb-and-sirt-1-nf-%C3%AE%C2%BAb-signaling-pathways
#19
Weidong Wang, Xiaofeng Liu, Jinping Liu, Enbo Cai, Yan Zhao, Haijun Li, Lianxue Zhang, Pingya Li, Yugang Gao
The previous study indicated sesquiterpenoids from the root of Panax ginseng (SPG) exhibited significant antidepressant-like effect, which might be mediated by the modification of the dopaminergic, GABAergic and glutamatergic systems. This study was to investigate SPG's antidepressant effects and mechanisms on lipopolysaccharide (LPS)-induced depression-like behavior. In the tail suspension test (TST) and forced swimming test (FST), SPG (0.25 and 1 mg/kg, i.g.) and fluoxetine (20 mg/kg, i.p.) effectively reduced the immobility time...
December 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29229735/oral-application-of-recombinant-bacillus-subtilis-spores-to-dogs-results-in-a-humoral-response-against-specific-echinococcus-granulosus-paramyosin-and-tropomyosin-antigens
#20
Cédric M Vogt, Maria Teresa Armúa-Fernández, Kurt Tobler, Monika Hilbe, Claudio Aguilar, Mathias Ackermann, Peter Deplazes, Catherine Eichwald
Bacillus subtilis is known as an endospore- and biofilm-forming bacterium with probiotic properties. We have recently developed a method for displaying heterologous proteins on the surface of B. subtilis biofilms by introducing the coding sequences of the protein of interest into the bacterial genome to generate a fusion protein linked to the C-terminus of the biofilm matrix protein TasA. Although B. subtilis is a regular component of the gut microflora, we constructed a series of recombinant B. subtilis strains that were tested for their ability to immunize dogs following oral application of the spores...
December 11, 2017: Infection and Immunity
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