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Tropomyosin

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https://www.readbyqxmd.com/read/29792862/the-reason-for-the-low-ca-2-sensitivity-of-thin-filaments-associated-with-the-glu41lys-mutation-in-the-tpm2-gene-is-freezing-of-tropomyosin-near-the-outer-domain-of-actin-and-inhibition-of-actin-monomer-switching-off-during-the-atpase-cycle
#1
Stanislava V Avrova, Olga E Karpicheva, Nikita A Rysev, Armen O Simonyan, Vladimir V Sirenko, Charles S Redwood, Yurii S Borovikov
The E41K mutation in TPM2 gene encoding muscle regulatory protein beta-tropomyosin is associated with nemaline myopathy and cap disease. The mutation results in a reduced Ca2+ -sensitivity of the thin filaments and in muscle weakness. To elucidate the structural basis of the reduced Ca2+ -sensitivity of the thin filaments, we studied multistep changes in spatial arrangement of tropomyosin (Tpm), actin and myosin heads during the ATPase cycle in reconstituted fibers, using the polarized fluorescence microscopy...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29775479/acute-intermittent-hypoxia-and-rehabilitative-training-following-cervical-spinal-injury-alters-neuronal-hypoxia-and-plasticity-associated-protein-expression
#2
Atiq Hassan, Breanna M Arnold, Sally Caine, Behzad M Toosi, Valerie M K Verge, Gillian D Muir
One of the most promising approaches to improve recovery after spinal cord injury (SCI) is the augmentation of spontaneously occurring plasticity in uninjured neural pathways. Acute intermittent hypoxia (AIH, brief exposures to reduced O2 levels alternating with normal O2 levels) initiates plasticity in respiratory systems and has been shown to improve recovery in respiratory and non-respiratory spinal systems after SCI in experimental animals and humans. Although the mechanism by which AIH elicits its effects after SCI are not well understood, AIH is known to alter protein expression in spinal neurons in uninjured animals...
2018: PloS One
https://www.readbyqxmd.com/read/29773804/smooth-muscle-like-ca-2-regulation-of-actin-myosin-interaction-in-adult-jellyfish-striated-muscle
#3
Hiroyuki Tanaka, Shiori Ishimaru, Yasuhiro Nagatsuka, Keisuke Ohashi
Cnidaria is an animal phylum, whose members probably have the most ancestral musculature. We prepared and characterized, for the first time to our knowledge, native actomyosin from the striated myoepithelium of the adult moon jelly Aurelia sp. The actomyosin contained myosin, paramyosin-like protein, Ser/Thr-kinase, actin, and two isoforms of tropomyosin, but not troponin, which is known to activate contraction dependent on intracellular Ca2+ signaling in almost all striated muscles of bilaterians. Notably, the myosin comprised striated muscle-type heavy chain and smooth muscle-type regulatory light chains...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29764494/precision-medicine-becomes-reality-tumor-type-agnostic-therapy
#4
REVIEW
Li Yan, Wei Zhang
Precision medicine just witnessed two breakthroughs in oncology in 2017. Pembrolizumab (Keytruda), Merck's anti-programmed cell death-1 (PD-1) monoclonal antibody (mAb), received accelerated approval in May 2017 by the US Food and Drug Administration for the treatment of adult and pediatric patients with unresectable or metastatic solid tumors that have been identified as having microsatellite instability-high (MSI-H) or deficient DNA mismatch repair (dMMR). Shortly after, nivolumab (Opdivo), Bristol-Myers Squibb's anti-PD-1 mAb, gained an accelerated approval in August 2017 for adult and pediatric patients with MSI-H or dMMR metastatic colorectal cancer that has progressed after standard chemotherapy...
March 31, 2018: Cancer communications
https://www.readbyqxmd.com/read/29760186/hypertrophic-cardiomyopathy-mutations-increase-myofilament-ca-2-buffering-alter-intracellular-ca-2-handling-and-stimulate-ca-2-dependent-signalling
#5
Paul Robinson, Xing Liu, Alexander Sparrow, Suketu Patel, Yin Hua Zhang, Barbara Casadei, Hugh Watkins, Charles S Redwood
Mutations in thin filament regulatory proteins that cause hypertrophic cardiomyopathy (HCM) increase myofilament Ca2+ -sensitivity. Mouse models exhibit increased Ca2+ buffering and arrhythmias, and we hypothesized that these changes are primary effects of the mutations (independent of compensatory changes) and that increased Ca2+ - buffering and altered Ca2+ -handling contribute to HCM pathogenesis via activation of Ca2+ -dependent signalling. Here, we determined the primary effects of HCM mutations on intracellular Ca2+ -handling and Ca2+ -dependent signalling in a model system possessing Ca2+ -handling mechanisms and contractile protein isoforms close to human in the absence of potentially confounding remodeling...
May 14, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29757584/effects-of-nerve-growth-factor-shrna-inhibition-on-asthma-phenotypes-in-a-mouse-model-of-asthma
#6
Jinhong Wu, Bing Chen, Wenxian Li, Yanan Xiao
Nerve growth factor (NGF) plays an important role in airway hyper-responsiveness (AHR). In this study, we aimed at investigating the effect of NGF inhibition on AHR and other asthma phenotypes in a mouse model of asthma. 12 mice in each group were injected with lentiviral vectors expressing non-targeting shRNA (sham shRNA), targeting NGF (shRNA-1 and shRNA-2), or normal saline for control before the asthma models were established. Peak inspiratory pressure (PIP), NGF levels in bronchoalveolar lavage fluid (BALF), and bronchoconstriction in response to acetylcholine (ACh) were measured...
April 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29756679/effect-of-heat-processing-on-ige-reactivity-and-cross-reactivity-of-tropomyosin-and-other-allergens-of-asia-pacific-mollusc-species-identification-of-novel-sydney-rock-oyster-tropomyosin-sac-g-1
#7
Jennifer M Rolland, Nirupama P Varese, Jodie B Abramovitch, Jessica Anania, Roni Nugraha, Sandip Kamath, Anita Hazard, Andreas L Lopata, Robyn E O'Hehir
SCOPE: Shellfish allergy is an increasing global health priority, frequently affecting adults. Molluscs are an important shellfish group causing food allergy but knowledge of their allergens and cross-reactivity is limited. Optimal diagnosis of mollusc allergy enabling accurate advice on food avoidance is difficult. We characterized allergens of four frequently ingested Asia-Pacific molluscs: Sydney Rock Oyster (Saccostrea glomerata), Blue Mussel (Mytilus edulis), Saucer Scallop (Amusium balloti) and Southern Calamari (Sepioteuthis australis), examining cross-reactivity between species and with Blue Swimmer Crab tropomyosin, Por p 1...
May 14, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29753231/vascular-brain-derived-neurotrophic-factor-pathway-in-rats-with-adjuvant-induced-arthritis-effect-of-anti-rheumatic-drugs
#8
Martin Pedard, Aurore Quirié, Perle Totoson, Frank Verhoeven, Philippe Garnier, Anne Tessier, Céline Demougeot, Christine Marie
BACKGROUND AND AIMS: In rheumatoid arthritis, the control of both disease activity and standard cardiovascular (CV) risk factors is expected to attenuate the increased CV risk. Evidence that brain-derived neurotrophic factor (BDNF) plays a role in vascular biology led us to investigate the vascular BDNF pathway in arthritis rats as well as the interaction between endothelial nitric oxide (NO) and BDNF production. METHODS: The aortic BDNF pathway was studied in rats with adjuvant-induced arthritis, (AIA) using Western blot and immunohistochemical analysis...
May 2, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29753009/the-implications-of-trka-and-met-aberrations-in-de-novo-salivary-duct-carcinoma
#9
Hyang Joo Ryu, Yoon Woo Koh, Sun Och Yoon
Salivary duct carcinoma (SDC) is an aggressive carcinoma with poor prognosis. Although anti-HER2 therapy is a potential treatment option for HER2-positive SDC, other potential therapeutic targets are not known, in particular for HER2-negative cases. In this study, the recently identified receptor tyrosine kinases MET and tropomyosin-receptor kinase (Trk) were investigated as potential therapeutic targets. A total of 28 consecutive, surgically resected, de novo SDC cases were selected after evaluating histology and immunohistochemical expression of androgen receptor...
May 9, 2018: Human Pathology
https://www.readbyqxmd.com/read/29752871/small-molecule-targeting-of-the-actin-associating-protein-tropomyosin-tpm3-1-increases-neuroblastoma-cell-response-to-rac-inhibition-of-multicellular-invasion
#10
Camilla B Mitchell, Justine R Stehn, Geraldine M O'Neill
The migration and invasion of cells through tissues in the body is facilitated by a dynamic actin cytoskeleton. The actin-associating protein, tropomyosin Tpm3.1 has emerged to play important roles in cell migration and invasion. To date, investigations have focused on single cell migration and invasion where Tpm3.1 expression is inversely associated with Rac GTPase-mediated cell invasion. While single cell and collective cell invasion have many features in common, collective invasion is additionally impacted by cell-cell adhesion, and the role of Tpm3...
May 12, 2018: Cytoskeleton
https://www.readbyqxmd.com/read/29752572/propolis-modulates-nos2-arginase-1-pathway-in-tropomyosin-induced-experimental-autoimmune-uveitis
#11
Kahina Touri, Houda Belguendouz, Oussama Medjeber, Zineb Djeraba, Karima Lahmar, Chafia Touil-Boukoffa
In this study, we evaluated the preventive and curative effects of ethanolic extract of Propolis (EEP) during α-Tropomyosin-induced uveitis in an experimental model using Wistar rats, through the regulation of inducible nitric oxide synthase (NOS2) and arginase-1. In this context, rats received daily injection of EEP (100 mg/kg) for 5 days prior to immunization or for 9 days commencing 5 days post immunization with α-Tropomyosin extract, then were sacrificed at day 14. Histological examination, NOS2, arginase-1, and nuclear factor-κB (NF-κB) expression were evaluated in the retinas...
May 11, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29752567/inhibiting-ezh2-rescued-bupivacaine-induced-neuronal-apoptosis-in-spinal-cord-dorsal-root-ganglia-in-mice
#12
Jinwei Zheng, Junping Chen, Guorong Wu, Chaoshuang Wu, Ruichun Wang, Wei Wang
PURPOSE: In the present work, we intended to explore the function of enhancer of zeste homolog 2 (EZH2) in modulated anesthetic reagent bupivacaine-induced neuronal apoptosis in spinal cord dorsal root ganglia (DRG). METHODS: Murine DRG explant was treated with 5 mM bupivacaine in vitro to induce neuronal apoptosis, which was examined by a TUNEL assay. Protein and mRNA expressions of EZH2 in bupivacaine-treated DRG were examined by western blot and qRT-PCR assays...
May 11, 2018: Journal of Anesthesia
https://www.readbyqxmd.com/read/29752279/a-comprehensive-study-of-calcific-aortic-stenosis-from-rabbit-to-human-samples
#13
L Mourino-Alvarez, M Baldan-Martin, T Sastre-Oliva, M Martin-Lorenzo, A S Maroto, N Corbacho-Alonso, R Rincon, T Martin-Rojas, L F Lopez-Almodovar, G Alvarez-Llamas, F Vivanco, L R Padial, F de la Cuesta, M G Barderas
The global incidence of calcific aortic stenosis (CAS) is increasing due, in part, to a growing elderly population, and poses a great challenge to public health because of the multiple comorbidities of these patients. Using a rabbit model of CAS, we sought to characterize protein alterations associated with calcified valve tissue that can be ultimately measured in plasma as non-invasive biomarkers of CAS.Aortic valves from healthy and mild stenotic rabbits were analyzed by two-dimensional difference gel electrophoresis, and selected reaction monitoring was used to directly measure the differentially expressed proteins in plasma from the same rabbits to corroborate their potential as diagnostic indicators, and also in plasma from human subjects, to examine their translatability to the clinical setting...
May 10, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29751919/effect-of-enzymatic-hydrolysis-on-bioactive-properties-and-allergenicity-of-cricket-gryllodes-sigillatus-protein
#14
Felicia Hall, Philip E Johnson, Andrea Liceaga
Food-derived bioactive peptides have gained attention for their role in preventing chronic diseases. Edible insects are viable sources of bioactive peptides owing to their high protein content and sustainable production. In this study, whole crickets (Gryllodes sigillatus) were alcalase-hydrolyzed to a degree of hydrolysis (DH) ranging from 15 to 85%. Antioxidant activity, angiotensin converting enzyme (ACE), and dipeptidyl peptidase-4 (DPP-IV)- inhibition of the cricket protein hydrolysates (CPH) were evaluated before and after simulated gastrointestinal digestion (SGD)...
October 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29750782/hey1-and-p53-dependent-trkc-proapoptotic-activity-controls-neuroblastoma-growth
#15
Marie Ménard, Clélia Costechareyre, Gabriel Ichim, Jonathan Blachier, David Neves, Loraine Jarrosson-Wuilleme, Reinhard Depping, Jan Koster, Pierre Saintigny, Patrick Mehlen, Servane Tauszig-Delamasure
The neurotrophin-3 (NT-3) receptor tropomyosin receptor kinase C (TrkC/NTRK3) has been described as a dependence receptor and, as such, triggers apoptosis in the absence of its ligand NT-3. This proapoptotic activity has been proposed to confer a tumor suppressor activity to this classic tyrosine kinase receptor (RTK). By investigating interacting partners that might facilitate TrkC-induced cell death, we have identified the basic helix-loop-helix (bHLH) transcription factor Hey1 and importin-α3 (karyopherin alpha 4 [KPNA4]) as direct interactors of TrkC intracellular domain, and we show that Hey1 is required for TrkC-induced apoptosis...
May 11, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29744662/co-expression-of-ngf-and-pd-l1-on-tumor-associated-immune-cells-in-the-microenvironment-of-merkel-cell-carcinoma
#16
Ulrike Wehkamp, Sophie Stern, Sandra Krüger, Michael Weichenthal, Axel Hauschild, Christoph Röcken, Friederike Egberts
PURPOSE: Merkel cell carcinoma (MCC) is a malignant neuroendocrine skin tumor with known viral association. The microenvironment and its interaction with the tumor via the programmed cell death protein 1 (PD-1) pathway are crucial for response to anti-PD-1/anti-PD-L1 treatments. However, not all patients respond, which is suggestive of additional mechanisms for tumor growth and/or persistence. We previously detected tropomyosin receptor kinase A (TrkA) expression on MCC tumor cells and, therefore, gained interest in the expression of its ligand nerve growth factor (NGF)...
May 9, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29739881/the-receptor-tyrosine-kinase-trkb-signals-without-dimerization-at-the-plasma-membrane
#17
Eitan Erez Zahavi, Noam Steinberg, Topaz Altman, Michael Chein, Yuvraj Joshi, Tal Gradus-Pery, Eran Perlson
Tropomyosin-related tyrosine kinase B (TrkB) is the receptor for brain-derived neurotrophic factor (BDNF) and provides critical signaling that supports the development and function of the mammalian nervous system. Like other receptor tyrosine kinases (RTKs), TrkB is thought to signal as a dimer. Using cell imaging and biochemical assays, we found that TrkB acted as a monomeric receptor at the plasma membrane regardless of its binding to BDNF and initial activation. Dimerization occurred only after the internalization and accumulation of TrkB monomers within BDNF-containing endosomes...
May 8, 2018: Science Signaling
https://www.readbyqxmd.com/read/29737479/locomotor-treadmill-training-promotes-soleus-trophism-by-mammalian-target-of-rapamycin-pathway-in-paraplegic-rats
#18
Caroline Cunha do Espírito Santo, Daniela Dal Secco, Anamaria Meireles, Gabriel Ribeiro de Freitas, Franciane Bobinski, Mauricio Peña Cunha, Ana Lúcia Severo Rodrigues, Alessandra Swarowsky, Adair Roberto Soares Santos, Jocemar Ilha
Assisted-treadmill training, may be helpful in promoting muscle mass preservation after incomplete spinal cord injury (SCI). However, biological mechanism involved in this process is still not fully understood. This study investigated the effects of locomotor treadmill training on muscle trophism mediated by protein kinase B (Akt)/mammalian target of rapamycin (mTOR)/p70 ribosomal protein S6 kinase (p70S6K) in paraplegic rats. Adult female Wistar rats underwent an incomplete thoracic SCI induced by compression using an aneurysm clip...
May 8, 2018: Neurochemical Research
https://www.readbyqxmd.com/read/29736744/antidepressant-efficacy-and-tolerability-of-ketamine-and-esketamine-a-critical-review
#19
REVIEW
P Molero, J A Ramos-Quiroga, R Martin-Santos, E Calvo-Sánchez, L Gutiérrez-Rojas, J J Meana
Ketamine and its enantiomer S-ketamine (esketamine) are promising candidates to produce a rapid-onset antidepressant effect in treatment-resistant depression. Ketamine causes continued blockade of the glutamate N-methyl-D-aspartate (NMDA) receptor, though this might not primarily mediate the antidepressant effect. Alternative hypotheses include selectivity for the NMDA receptor subtype containing the NMDA receptor subunit 2B (NR2B), inhibition of the phosphorylation of the eukaryotic elongation factor 2 (eEF2) kinase, increased expression of brain-derived neurotrophic factor (BDNF) and tropomyosin receptor kinase B (TrKB), and activation of the mammalian target of rapamycin (mTOR) signaling pathway, alongside other independent actions attributed to the ketamine metabolism to R-hydroxynorketamine (R-HNK)...
May 7, 2018: CNS Drugs
https://www.readbyqxmd.com/read/29731872/tropomyosin-1-promotes-cancer-cell-apoptosis-via-the-p53-mediated-mitochondrial-pathway-in-renal-cell-carcinoma
#20
Chao Tang, Jin Wang, Qi Wei, Yi-Peng Du, He-Ping Qiu, Chao Yang, Yu-Chuan Hou
Tropomyosin-1 (TPM1), a widely expressed actin-binding protein, is downregulated in many tumors and associated with cancer progression. A previous study from our group suggested that TPM1 could be involved in renal cell carcinoma (RCC) apoptosis, but the mechanisms and details remained unknown. The present study aimed to further examine the proapoptotic effects of TPM1 and investigate the underlying mechanisms in RCC cell lines. Results from cell viability, DAPI staining and apoptosis assays demonstrated that TPM1 upregulation inhibited cell proliferation and promoted cell apoptosis in both 786-O and ACHN RCC cell lines...
May 2018: Oncology Letters
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