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Myelin basic protein

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https://www.readbyqxmd.com/read/29155726/visualization-and-live-imaging-of-oligodendrocyte-organelles-in-organotypic-brain-slices-using-adeno-associated-virus-and-confocal-microscopy
#1
Lauritz Hagen Kennedy, Johanne Egge Rinholm
Neurons rely on the electric insulation and trophic support of myelinating oligodendrocytes. Despite the importance of oligodendrocytes, the advanced tools currently used to study neurons, have only partly been taken on by oligodendrocyte researchers. Cell type-specific staining by viral transduction is a useful approach to study live organelle dynamics. This paper describes a protocol for visualizing oligodendrocyte mitochondria in organotypic brain slices by transduction with adeno-associated virus (AAV) carrying genes for mitochondrial targeted fluorescent proteins under the transcriptional control of the myelin basic protein promoter...
October 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29143164/upregulation-of-myelin-gene-expression-by-a-physically-modified-saline-via-phosphatidylinositol-3-kinase-mediated-activation-of-creb-implications-for-multiple-sclerosis
#2
Malabendu Jana, Supurna Ghosh, Kalipada Pahan
An increase in central nervous system (CNS) remyelination and a decrease in CNS inflammation are important steps to halt the progression of multiple sclerosis (MS). RNS60 is a bioactive aqueous solution generated by subjecting normal saline to Taylor-Couette-Poiseuille flow under elevated oxygen pressure. Recently we have demonstrated that RNS60 exhibits anti-inflammatory properties. Here, we describe promyelinating property of RNS60. RNS60, but not normal saline (NS), RNS10.3 (TCP-modified saline without excess oxygen) or PNS60 (saline containing excess oxygen without TCP modification), stimulated the expression of myelin-specific genes and proteins (myelin basic protein, MBP; myelin oligodendrocyte glycoprotein, MOG and proteolipid protein, PLP) in primary mouse oligodendroglia and mixed glial cells...
November 15, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/29128575/strain-specificities-in-age-related-changes-in-mechanisms-promoting-and-controlling-rat-spinal-cord-damage-in-experimental-autoimmune-encephalomyelitis
#3
Zorica Stojić-Vukanić, Ivan Pilipović, Jasmina Djikić, Ivana Vujnović, Mirjana Nacka-Aleksić, Biljana Bufan, Nevena Arsenović-Ranin, Duško Kosec, Gordana Leposavić
The study investigated strain specificities in age-related differences in CD8+ T cell- and microglial cell-mediated mechanisms implicated in induction/perpetuation and/or control of neuroinflammation in experimental autoimmune encephalomyelitis (EAE) in Albino Oxford (AO) and Dark Agouti (DA) rats exhibiting age-related changes in the susceptibility to EAE in the opposite direction (increase in relatively resistant AO rats vs decrease in DA rats). In the inductive phase of EAE, the greater number of fully differentiated effector CD8+ T lymphocytes was found in draining lymph nodes (dLNs) from aged rats of both strains than in strain-matched young rats, but this was particularly prominent in AO rats, which exhibited milder EAE of prolonged duration compared with their DA counterparts...
November 8, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/29126442/effect-of-enriched-housing-on-levels-of-natural-auto-antibodies-in-pigs-co-infected-with-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-and-actinobacillus-pleuropneumoniae
#4
Lu Luo, Ingrid Daniëlle Ellen van Dixhoorn, Inonge Reimert, Bas Kemp, Jantina Elizabeth Bolhuis, Hendrik Karel Parmentier
Housing of pigs in barren, stimulus-poor housing conditions may influence their immune status, including antibody responses to (auto-)antigens, and thus affect immune protection, which will influence the onset and outcome of infection. In the present study, we investigated the effects of environmental enrichment versus barren housing on the level of natural (auto-)antibodies (NA(A)b) and their isotypes (IgM and IgG) binding keyhole limpet hemocyanin (KLH), myelin basic protein (MBP), and phosphorycholine conjugated to bovine serum albumin (PC-BSA) in pigs co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and Actinobacillus pleuropneumoniae (A...
November 10, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29111742/increased-post-translational-lysine-acetylation-of-myelin-basic-protein-is-associated-with-peak-neurological-disability-in-a-mouse-experimental-autoimmune-encephalomyelitis-model-of-multiple-sclerosis
#5
Ryan Lillico, Ting Zhou, Tina Khorshid Ahmad, Nicholas Stesco, Kiana Gozda, Jessica Truong, Jiming Kong, Ted M Lakowski, Michael Namaka
Citrullination of arginine residues is a post-translational modification (PTM) found on myelin basic protein (MBP), which neutralizes MBPs positive charge, and is implicated in myelin damage and multiple sclerosis (MS). Here we identify lysine acetylation as another neutralizing PTM to MBP that may be involved in myelin damage. We quantify changes in lysine and arginine PTMs on MBP derived from mice induced with an experimental autoimmune encephalomyelitis (EAE) model of MS using liquid chromatography tandem mass spectrometry...
November 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29104800/caffeoylquinic-acid-enhances-proliferation-of-oligodendrocyte-precursor-cells
#6
Ying Yanqin, Chen Shaohua, Tang Jing, Li Nan
This report evaluates the protective effect of caffeoylquinic acid (CA) injury to oligodendrocyte precursor cells (OPCs) by promoting the formation of oligodendrocytes. Neonatal rat brain was used to isolate primary OPCs and non-lethal CoCl2 was used to induce hypoxic stress to inhibit the differentiation of OPCs. Differentiation of OPCs was estimated by survival assay and the expressions of myelin-basic-protein (MBP). Moreover, the effect of CA on the Akt signanling pathway was also estimated in the presence and absence of LY294002 (PI3K/Akt inhibitor) and adrenomedullin (AM) receptor antagonist (AM22-52) by using western blot assay...
2017: Translational Neuroscience
https://www.readbyqxmd.com/read/29096669/anti-carbamylated-protein-antibodies-precede-disease-onset-in-monkeys-with-collagen-induced-arthritis
#7
Marije K Verheul, Michel P M Vierboom, Bert A 't Hart, Rene E M Toes, Leendert A Trouw
BACKGROUND: Rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPA) and anti-carbamylated protein (anti-CarP) antibodies are rheumatoid arthritis (RA)-associated autoantibodies. Besides their presence in human serum, anti-CarP antibodies have also been described in rodent models of arthritis, while ACPA are not consistently detectable. Data on these RA-associated autoantibodies in primates are not available. Therefore, we investigated the presence of RF, anti-CarP antibodies and ACPA in rhesus monkeys before and after collagen-induced arthritis immunizations...
November 2, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/29095858/phospholipase-a2-is-involved-in-galactosylsphingosine-induced-astrocyte-toxicity-neuronal-damage-and-demyelination
#8
Cedric Misslin, Maria Velasco-Estevez, Marie Albert, Sinead A O'Sullivan, Kumlesh K Dev
Krabbe disease is a fatal rare inherited lipid storage disorder affecting 1:100,000 births. This illness is caused by mutations in the galc gene encoding for the enzyme galactosylceramidase (GALC). Dysfunction of GALC has been linked to the toxic build-up of the galactolipid, galactosylsphingosine (psychosine), which induces cell death of oligodendrocytes. Previous studies show that phospholipase A2 (PLA2) may play a role in psychosine induce cell death. Here, we demonstrate that non-selective inhibition of cPLA2/sPLA2 and selective inhibition of cPLA2, but not sPLA2, also attenuates psychosine-induced cell death of human astrocytes...
2017: PloS One
https://www.readbyqxmd.com/read/29076559/genetic-influences-on-cognition-in-progressive-supranuclear-palsy
#9
Adam Gerstenecker, Erik D Roberson, Gerard D Schellenberg, David G Standaert, David R Shprecher, Benzi M Kluger, Irene Litvan
BACKGROUND: Cognitive dysfunction is common in progressive supranuclear palsy, but the influence of genetics on cognition in this disorder has not been well studied. The objective of this study was to investigate the effect of genes previously identified as risk alleles, including microtubule-associated protein tau, myelin-associated oligodendrocyte basic protein, eukaryotic translation initiation factor 2-alpha kinase 3, and syntaxin 6, as well as apolipoprotein E, on cognitive function in progressive supranuclear palsy...
October 27, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29075188/high-yield-of-adult-oligodendrocyte-lineage-cells-obtained-from-meningeal-biopsy
#10
Sissi Dolci, Annachiara Pino, Valeria Berton, Pau Gonzalez, Alice Braga, Marta Fumagalli, Elisabetta Bonfanti, Giorgio Malpeli, Francesca Pari, Stefania Zorzin, Clelia Amoroso, Denny Moscon, Francisco J Rodriguez, Guido Fumagalli, Francesco Bifari, Ilaria Decimo
Oligodendrocyte loss can lead to cognitive and motor deficits. Current remyelinating therapeutic strategies imply either modulation of endogenous oligodendrocyte precursors or transplantation of in vitro expanded oligodendrocytes. Cell therapy, however, still lacks identification of an adequate source of oligodendrocyte present in adulthood and able to efficiently produce transplantable cells. Recently, a neural stem cell-like population has been identified in meninges. We developed a protocol to obtain high yield of oligodendrocyte lineage cells from one single biopsy of adult rat meningeal tissue...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29073112/dynein-dynactin-is-necessary-for-anterograde-transport-of-mbp-mrna-in-oligodendrocytes-and-for-myelination-in-vivo
#11
Amy L Herbert, Meng-Meng Fu, Catherine M Drerup, Ryan S Gray, Breanne L Harty, Sarah D Ackerman, Thomas O'Reilly-Pol, Stephen L Johnson, Alex V Nechiporuk, Ben A Barres, Kelly R Monk
Oligodendrocytes in the central nervous system produce myelin, a lipid-rich, multilamellar sheath that surrounds axons and promotes the rapid propagation of action potentials. A critical component of myelin is myelin basic protein (MBP), expression of which requires anterograde mRNA transport followed by local translation at the developing myelin sheath. Although the anterograde motor kinesin KIF1B is involved in mbp mRNA transport in zebrafish, it is not entirely clear how mbp transport is regulated. From a forward genetic screen for myelination defects in zebrafish, we identified a mutation in actr10, which encodes the Arp11 subunit of dynactin, a critical activator of the retrograde motor dynein...
October 24, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29071135/grifolin-attenuates-white-matter-lesion-in-oxygen-glucose-deprivation
#12
Ying Yanqin, Tang Jing, Chen Wei, Li Nan
The present study evaluates the effect of grifolin (GFL) in oxygen/glucose deprivation (OGD) induced white matter lesion. Injury induced with OGD was found to be significant at the 9th h of OGD induction and the effect of GFL on the proliferation of oligodendrocyte precursor cells (OPCs) was assessed by CCK-8 and Hoechst 33258 assay at GFL 1, 5, 25, 50 and 100 μm concentrations. Whereas immunocytochemistry was performed for the assessment of survival and apoptosis of OPCs, western blot assay and RT-PCR were performed after 8th day of OGD injury for the estimation of expressions of myelin basic protein (MBP) and inhibitor of DNA binding 2 (Id2) in OPCs respectively...
2017: Translational Neuroscience
https://www.readbyqxmd.com/read/29069985/neural-autoantibodies-in-patients-with-neurological-symptoms-and-histories-of-chemical-mold-exposures
#13
Mohamed Bahie Abou-Donia, Allan Lieberman, Luke Curtis
A number of studies have linked exposures to industrial and household chemicals and biological toxins to increased risk of autoimmunity in general and elevated levels of autoantibodies to neural antigens specifically. Elevated neural autoantibodies are biomarkers for many diseases such as multiple sclerosis and Parkinson's disease. Our study reports levels of six types of neural autoantibodies in a group of 24 toxicant-exposed patients. The patients were exposed to a variety of toxicants including contaminated drinking water (four patients), building water/mold damage (eight patients), pesticides (four patients), and other assorted toxic chemicals (eight patients)...
January 1, 2017: Toxicology and Industrial Health
https://www.readbyqxmd.com/read/29058769/lewis-rat-model-of-experimental-autoimmune-encephalomyelitis
#14
Kalliopi Pitarokoili, Bjoern Ambrosius, Ralf Gold
In this unit, we describe in detail the most common methods used to break immunological tolerance for central myelin antigens and induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats as an animal model of multiple sclerosis. The resulting disease course ranges from an acute monophasic disease to a chronic relapsing or chronic progressive course, which strongly resembles the human disease. These models enable the study of cellular and humoral autoimmunity against major antigenic epitopes of the myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein...
October 23, 2017: Current Protocols in Neuroscience
https://www.readbyqxmd.com/read/29053067/the-proteome-of-pus-from-human-brain-abscesses-host-derived-neurotoxic-proteins-and-the-cell-type-diversity-of-cns-pus
#15
Bjørnar Hassel, Gustavo Antonio De Souza, Maria Ekman Stensland, Jugoslav Ivanovic, Øyvind Voie, Daniel Dahlberg
OBJECTIVE What determines the extent of tissue destruction during brain abscess formation is not known. Pyogenic brain infections cause destruction of brain tissue that greatly exceeds the area occupied by microbes, as seen in experimental studies, pointing to cytotoxic factors other than microbes in pus. This study examined whether brain abscess pus contains cytotoxic proteins that might explain the extent of tissue destruction. METHODS Pus proteins from 20 human brain abscesses and, for comparison, 7 subdural empyemas were analyzed by proteomics mass spectrometry...
October 20, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29051083/lysophosphatidic-acid-propagates-post-injury-schwann-cell-dedifferentiation-through-lpa1-signaling
#16
Fabian Szepanowski, Leon-Phillip Szepanowski, Anne K Mausberg, Christoph Kleinschnitz, Bernd C Kieseier, Mark Stettner
Lysophosphatidic acid (LPA) is a pleiotropic signaling lipid that acts as ligand for at least six specific G-protein coupled receptors. Schwann cells (SC) are known to mainly express the LPA1 receptor subtype. An emerging body of evidence has linked LPA with injury-induced peripheral nerve demyelination as well as neuropathic pain. However, the molecular mechanisms underlying its demyelinating effect have not been conclusively elucidated. We aimed to decipher the demyelinating effect in vitro as well as in vivo by studying markers of SC differentiation and dedifferentiation: Myelinated dorsal root ganglia (DRG) cultures were treated either with LPA, LPA plus AM095 (LPA1 antagonist) or vehicle...
October 16, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/29046699/age-dependent-differences-in-myelin-basic-protein-expression-in-the-hippocampus-of-young-adult-and-aged-gerbils
#17
Ji Hyeon Ahn, Tae-Kyeong Lee, Joon Ha Park, Jeong Hwi Cho, In Hye Kim, Jae Chul Lee, Seongkweon Hong, Yong Hwan Jeon, Il Jun Kang, Young Joo Lee, Moo-Ho Won, Choong-Hyun Lee
Myelin degeneration is one of the characteristics of aging and degenerative diseases. This study investigated age-related alterations in expression of myelin basic protein (MBP) in the hippocampal subregions (dentate gyrus, CA2/3 and CA1 areas) of gerbils of various ages; young (1 month), adult (6 months) and aged (24 months), using western blot and immunohistochemistry. Western blot results showed tendencies of age-related reductions of MBP levels. MBP immunoreactivity was significantly decreased with age in synaptic sites of trisynaptic loops, perforant paths, mossy fibers, and Schaffer collaterals...
September 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/29038005/prednisone-alleviates-demyelination-through-regulation-of-the-nlrp3-inflammasome-in-a-c57bl-6-mouse-model-of-cuprizone-induced-demyelination
#18
Hao Yu, Mingfeng Wu, Geng Lu, Tingting Cao, Nan Chen, Yijia Zhang, Higuo Jiang, Hongbin Fan, Ruiqin Yao
Myelin abnormalities, oligodendrocyte damage, and concomitant glia activation are common in demyelinating diseases of the central nervous system (CNS). Increasing evidence has demonstrated that the inflammatory response triggers demyelination and gliosis in demyelinating disorders. Numerous clinical interventions, including those used to treat multiple sclerosis (MS), have confirmed prednisone (PDN) as a powerful anti-inflammatory drug that reduces the inflammatory response and promotes tissue repair in multiple inflammation sites...
October 13, 2017: Brain Research
https://www.readbyqxmd.com/read/29037840/comparison-of-plasma-copeptin-and-multiple-biomarkers-for-assessing-prognosis-of-patients-with-aneurysmal-subarachnoid-hemorrhage
#19
Yong-Ke Zheng, Xiao-Qiao Dong, Quan Du, Hao Wang, Ding-Bo Yang, Qiang Zhu, Zhi-Hao Che, Yong-Feng Shen, Li Jiang, Wei Hu, Ke-Yi Wang, Wen-Hua Yu
BACKGROUND: Increased plasma copeptin concentrations are related to poor prognosis after aneurysmal subarachnoid hemorrhage (aSAH). The aim of this study was to assess prognostic significance of plasma copeptin detection compared with glial fibrillary astrocyte protein, myelin basic protein, S100B, phosphorylated axonal neurofilament subunit H, neuron-specific enolase, tau and ubiquitin carboxyl-terminal hydrolase L1 in aSAH. METHODS: We detected plasma concentrations of the aforementioned biomarkers in 105 healthy controls using ELISA...
October 13, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29028399/is-myelin-basic-protein-a-potential-biomarker-of-brain-cancer
#20
M G Zavialova, V E Shevchenko, E N Nikolaev, V G Zgoda
Myelin basic protein is a potential biomarker for the central nervous system diseases in which the myelin sheath is destroyed. Using pseudo-selected reaction monitoring and the method of standard additions, we have measured the myelin basic protein level in the cerebrospinal fluid of patients with neurotrauma (n = 6), chronic neurodegenerative diseases (n = 2) and brain cancer (n = 5). Myelin basic protein was detected only in four out of five cerebrospinal fluid samples of patients with brain cancer...
August 2017: European Journal of Mass Spectrometry
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