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https://www.readbyqxmd.com/read/28316949/evaluation-of-the-role-of-probiotics-in-endodontic-treatment-a-preliminary-study
#1
Aarti Bohora, Sharad Kokate
AIMS AND OBJECTIVES: The principal goal of endodontics is the prevention of periapical infection. Acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as Enterococcus faecalis and Candida albicans harboring the root canal systems of the teeth. The concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. On the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to evaluate the antibacterial efficacy of probiotics against common endodontic pathogens, i...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28301013/influence-of-different-dentin-substrate-caries-affected-caries-infected-sound-on-long-term-%C3%AE-tbs
#2
Ana Rosa Costa, Franklin Garcia-Godoy, Lourenço Correr-Sobrinho, Lucas Zago Naves, Luís Henrique Araújo Raposo, Fabíola Galbiatti de Carvalho, Mário Alexandre Coelho Sinhoreti, Regina Maria Puppin-Rontani
The aim of this study was to evaluate the μTBS in different dentin substrates and water-storage periods. Twenty-four dentin blocks obtained from sound third molars were randomly divided into 3 groups: Sound dentin (Sd), Caries-affected dentin (Ca) and Caries-infected dentin (Ci). Dentin blocks from Ca and Ci groups were subjected to artificial caries development (S. mutans biofilm). The softest carious tissue was removed using spherical drills under visual inspection with Caries Detector solution (Ca group)...
January 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28291433/effects-of-bone-cement-loaded-with-teicoplanin-n-acetylcysteine-or-their-combination-on-staphylococcus-aureus-biofilm-formation-an-in-vitro-study
#3
Hasan Göçer, Dilek Emir, Mehmet Emin Önger, Nevzat Dabak
OBJECTIVES: This study aims to demonstrate the antibiofilm effects of teicoplanin alone, N-acetyl cysteine (NAC) alone, or combination of both compounds when mixed with bone cement. MATERIALS AND METHODS: A total of four groups were formed by using six cement samples in each, prepared with bone cement having different contents in each group. Group 1 (control group): cement alone without any drugs added. Group 2: 40 g cement, 400 mg teicoplanin. Group 3: 40 g cement, 6 g NAC...
April 2017: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/28288426/microbiome-involved-in-microbial-electrochemical-systems-mess-a-review
#4
Rijuta Ganesh Saratale, Ganesh Dattatraya Saratale, Arivalagan Pugazhendhi, Guangyin Zhen, Gopalakrishnan Kumar, Abudukeremu Kadier, Periyasamy Sivagurunathan
Microbial electrochemical systems (MESs) are an attracting technology for the disposal of wastewater treatment and simultaneous energy production. In MESs, at the anode microorganisms through the catalytic activity generates electrons that can be converted into electricity or other valuable chemical compounds. Microorganisms those having ability to donate and accept electrons to and from anode and cathode electrodes, respectively are recognized as 'exoelectrogens'. In the MESs, it renders an important function for its performance...
March 9, 2017: Chemosphere
https://www.readbyqxmd.com/read/28284446/biofiltration-of-xylene-using-wood-charcoal-as-the-biofilter-media-under-transient-and-high-loading-conditions
#5
Kiran Singh, B S Giri, Amrita Sahi, S R Geed, M K Kureel, Sanjay Singh, S K Dubey, B N Rai, Surendra Kumar, S N Upadhyay, R S Singh
The main objective of this study was to evaluate the performance of wood charcoal as biofilter media under transient and high loading condition. Biofiltration of xylene was investigated for 150days in a laboratory scale unit packed with wood charcoal and inoculated with mixed microbial culture at the xylene loading rates ranged from 12 to 553gm(-3)h(-1). The kinetic analysis of the xylene revealed absence of substrate inhibition and possibility of achieving higher elimination under optimum condition. The pH, temperature, pressure drop and CO2 production rate were regularly monitored during the experiments...
February 21, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28278777/improvement-of-start-up-and-nitrogen-removal-of-the-anammox-process-in-reactors-inoculated-with-conventional-activated-sludge-using-biofilm-carrier-materials
#6
Yi-Feng Lu, Li-Juan Ma, Lan Ma, Bei Shan, Jun-Jun Chang
The start-up of the anaerobic ammonium oxidation (anammox) process in three up-flow column reactors seeded with common mixed activated sludge and added with three materials, sponge (R1), sponge + volcanic rock (R2) and sponge + charcoal (R3), as carriers for biofilm formation were comparatively investigated in this study. The supplement of volcanic rock and charcoal could significantly shorten the start-up time of the anammox process, which primarily occurred in the activity-enhanced phase, with ammonium and nitrite removal efficiencies stabilized above 92...
February 28, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28259434/effect-of-yeasts-on-biodegradation-potential-of-immobilized-cultures-of-white-rot-fungi
#7
Pavlína Šlosarčíková, Čeněk Novotný, Kateřina Malachová, Hana Válková, Jindřich Fojtík
The aim was to investigate the effect of yeast organisms on the degradation process by immobilized cultures of ligninolytic fungi. Immobilization was accomplished by 7-day colonization of polyamide mesh with mycelial fragments. Irpex lacteus decolorized >90% of the initial concentration of 150mgl(-1) of anthraquinone Remazol Brilliant Blue R dye in three subsequent decolorization cycles and the degradation capacity was not negatively affected by the presence of 10(6)Saccharomyces cerevisiae cells per ml in the mixed culture...
March 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28256700/clinical-relevance-of-antimicrobial-testing-results-for-dental-restorative-materials
#8
Cher Farrugia, Julie Haider, Liberato Camilleri, Josette Camilleri
BACKGROUND: The antimicrobial activity of restorative materials is clinically relevant because all dental materials are subject to an environment containing bacteria. This study aimed to investigate the use of 2 methodologies referred to in the literature to assess antimicrobial properties of restorative materials and investigate whether material properties alter results of these traditional methodologies. METHODS: A number of dental restorative materials - namely, Chemfil Superior®, Spectrum®, Heliobond®, Ionoseal®, Dyract Extra®, Smart Dentin Replacement (SDR®) and Biodentine® - were characterized by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), X-ray diffraction (XRD) analysis, Fourier transform infrared (FT-IR) spectroscopy and pH analysis...
February 28, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/28254049/performance-of-low-temperature-microbial-fuel-cells-mfcs-catalyzed-by-mixed-bacterial-consortia
#9
Olga Tkach, Thangavel Sangeetha, Spiridonova Maria, Aijie Wang
Microbial Fuel Cells (MFCs) are a promising technology for treating wastewater in a sustainable manner. In potential applications, low temperatures substantially reduce MFC performance. To better understand the effect of temperature and particularly how bioanodes respond to changes in temperature, we investigated the current generation of mixed-culture and pure-culture MFCs at two low temperatures, 10°C and 5°C. The results implied that the mixed-culture MFC sustainably performed better than the pure-culture (Shewanella) MFC at 10°C, but the electrogenic activity of anodic bacteria was substantially reduced at the lower temperature of 5°C...
February 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28237766/n-acetylcysteine-inhibits-growth-adhesion-and-biofilm-formation-of-gram-positive-skin-pathogens
#10
Daria Eroshenko, Tatyana Polyudova, Vladimir Korobov
N-Acetylcysteine (NAC) is a non-antibiotic drug with antimicrobial properties against biofilm phenotypes of Gram-positive and Gram-negative bacteria. Our aim was to assess the effects of NAC on the growth of Gram-positive human skin and mucous membrane pathogens in the planktonic and biofilm phases. The minimum inhibitory concentrations (MICs) of NAC against Enterococcus faecalis, Corynebacterium ammoniagenes, Mycobacterium smegmatis, Propionibacterium acnes, Staphylococcus aureus, S. epidermidis, Streptococcus pyogenes, and 14 clinical strains of coagulase-negative staphylococci (CNS) ranged from 0...
February 22, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28198976/role-of-candida-species-from-hiv-infected-children-in-enamel-caries-lesions-an-in-vitro-study
#11
Senda Charone, Maristela Barbosa Portela, Karol de Oliveira Martins, Rosangela Maria Soares, Gloria Fernanda Castro
Objectives: This study analyzed the capacity of Candida spp. from dental biofilm of HIV infected (HIV+) children to demineralize primary molar enamel in vitro by Transversal Microhardness (TMH), Polarized Light Microscopy (PLM) and the quantity of calcium ions (Ca2+) released from the enamel. Material and Methods: Candida spp. samples were isolated from the supragingival biofilm of HIV+ children. A hundred and forty (140) enamel blocks were randomly assigned to six groups: biofilm formed by C...
January 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28194955/luminometric-label-array-for-counting-and-differentiation-of-bacteria
#12
Milla Högmander, Catherine J Paul, Sandy Chan, Elina Hokkanen, Ville Eskonen, Tapio Pahikkala, Sari Pihlasalo
Methods for simple and fast detection and differentiation of bacterial species are required, for instance, in medicine, water quality monitoring, and the food industry. Here, we have developed a novel label array method for the counting and differentiation of bacterial species. This method is based on the nonspecific interactions of multiple unstable lanthanide chelates and selected chemicals within the sample leading to a luminescence signal profile that is unique to the bacterial species. It is simple, cost-effective, and/or user-friendly compared to many existing methods, such as plate counts on selective media, automatic (hemocytometer-based) cell counters, flow cytometry, and polymerase chain reaction (PCR)-based methods for identification...
February 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28187935/effect-of-bacteria-on-the-degradation-ability-of-pleurotus-ostreatus
#13
Hana Válková, Čeněk Novotný, Kateřina Malachová, Pavlína Šlosarčíková, Jindřich Fojtík
White-rot fungi are efficient degraders of lignin whose extracellular enzymes have a potential to degrade organopollutants. In natural conditions these fungi enter into interactions with other organisms, which may affect their biodegradation capacity. The aim was to investigate the ability of Pleurotus ostreatus to form stable biofilms and to test the capacity of the fungus to degrade Remazol Brilliant Blue R in mixed cultures with bacteria. Bacterial counts were determined to see the behavior of the bacterium in the mixed culture with the fungus...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28173753/conjugative-type-ivb-pilus-recognizes-lipopolysaccharide-of-recipient-cells-to-initiate-papi-1-pathogenicity-island-transfer-in-pseudomonas-aeruginosa
#14
Toan Phuoc Hong, Michelle Q Carter, Paolo Struffi, Stefano Casonato, Youai Hao, Joseph S Lam, Stephen Lory, Olivier Jousson
BACKGROUND: Pseudomonas aeruginosa pathogenicity island 1 (PAPI-1) is one of the largest genomic islands of this important opportunistic human pathogen. Previous studies have shown that PAPI-1 encodes several putative virulence factors, including a major regulator of biofilm formation and antibiotic-resistance traits. PAPI-1 is horizontally transferable into recipient strains lacking this island via conjugation mediated by the specialized type IV pilus. The PAPI-1 encodes a cluster of ten genes associated with the synthesis and assembly of the type IV pilus...
February 7, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28164244/candida-krusei-and-candida-glabrata-reduce-the-filamentation-of-candida-albicans-by-downregulating-expression-of-hwp1-gene
#15
Patrícia Pimentel de Barros, Fernanda Freire, Rodnei Dennis Rossoni, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C...
February 6, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28161488/cold-atmospheric-pressure-plasma-elimination-of-clinically-important-single-and-mixed-species-biofilms
#16
Martina Modic, Neil P McLeod, J Mark Sutton, James L Walsh
Mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. An indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) pathogens. Plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (Enterococcus faecalis and Klebsiella pneumoniae) was considered...
March 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28160677/nutrient-and-suspended-solids-removal-from-petrochemical-wastewater-via-microalgal-biofilm-cultivation
#17
Alan Hodges, Zachary Fica, Jordan Wanlass, Jessica VanDarlin, Ronald Sims
Wastewater derived from petroleum refining currently accounts for 33.6 million barrels per day globally. Few wastewater treatment strategies exist to produce value-added products from petroleum refining wastewater. In this study, mixed culture microalgal biofilm-based treatment of petroleum refining wastewater using rotating algae biofilm reactors (RABRs) was compared with suspended-growth open pond lagoon reactors for removal of nutrients and suspended solids. Triplicate reactors were operated for 12 weeks and were continuously fed with petroleum refining wastewater...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28156000/rhamnolipids-from-pseudomonas-aeruginosa-strain-w10-as-antibiofilm-antibiofouling-products-for-metal-protection
#18
Alif Chebbi, Mohamed Elshikh, Farazul Haque, Syed Ahmed, Sara Dobbin, Roger Marchant, Sami Sayadi, Mohamed Chamkha, Ibrahim M Banat
Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L(-1) and a critical micelle concentration (CMC) of 80 mg L(-1) . P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28118640/inhibition-of-streptococcus-mutans-growth-and-biofilm-formation-by-probiotics-in-vitro
#19
Falk Schwendicke, Franziska Korte, Christof E Dörfer, Susanne Kneist, Karim Fawzy El-Sayed, Sebastian Paris
To exert anticaries effects, probiotics are described to inhibit growth and biofilm formation of cariogenic bacteria such as Streptococcus mutans (SM). We screened 8 probiotics and assessed how SM growth or biofilm formation inhibition affects cariogenicity of probiotic-SM mixed-species biofilms in vitro. Growth inhibition was assessed by cocultivating probiotics and 2 SM strains (ATCC 20532/25175) on agar. Probiotics were either precultured before SM cultivation (exclusion), or SM precultured prior to probiotic cultivation (displacement)...
2017: Caries Research
https://www.readbyqxmd.com/read/28117832/salivary-mucins-promote-the-coexistence-of-competing-oral-bacterial-species
#20
Erica Shapiro Frenkel, Katharina Ribbeck
Mucus forms a major ecological niche for microbiota in various locations throughout the human body such as the gastrointestinal tract, respiratory tract and oral cavity. The primary structural components of mucus are mucin glycoproteins, which crosslink to form a complex polymer network that surrounds microbes. Although the mucin matrix could create constraints that impact inhabiting microbes, little is understood about how this key environmental factor affects interspecies interactions. In this study, we develop an experimental model using gel-forming human salivary mucins to understand the influence of mucin on the viability of two competing species of oral bacteria...
January 24, 2017: ISME Journal
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