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Mixed biofilm

Yulong Tan, Matthias Leonhard, Doris Moser, Su Ma, Berit Schneider-Stickler
OBJECTIVES: Oral candidiasis is one of the most common human fungal infections. While most cases of the Candida species isolated from the oral cavity are Candida albicans, a large number of candidiasis is attributed to non-albicans Candida species. In this study, we aim to evaluate the in vitro inhibition of supernatants of Lactobacillus gasseri and Lactobacillus rhamnosus on the single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida krusei and Candida parapsilosis...
October 6, 2017: Archives of Oral Biology
Irina Spacova, Elke Lievens, Tine Verhoeven, Hans Steenackers, Jos Vanderleyden, Sarah Lebeer, Mariya I Petrova
Probiotic Lactobacillus strains are widely used to benefit human and animal health, although the exact mechanisms behind their interactions with the host and the microbiota are largely unknown. Fluorescent tagging of live probiotic cells is an important tool to unravel their modes of action. In this study, the implementation of different heterologously expressed fluorescent proteins for the labelling of the model probiotic strains Lactobacillus rhamnosusGG (gastrointestinal) and Lactobacillus rhamnosusGR-1 (vaginal) was explored...
October 13, 2017: Microbial Biotechnology
Oliver Heller, Roger Stephan, Sophie Thanner, Michael Hässig, Giuseppe Bee, Andreas Gutzwiller, Xaver Sidler
BACKGROUND: Drugs for the treatment of groups of pigs receiving liquid feed are frequently mixed into the feed and administered via the pipelines of the feeding installations. In-feed antimicrobials may select antimicrobial resistant strains among the bacteria which form the biofilm of these pipelines and are shed into the liquid feed. OBJECTIVE AND METHODS: In order to evaluate the risk of selecting antimicrobial resistant bacteria in the biofilm of liquid feeding installations, the effect of the administration of antimicrobials via the pipelines on the occurrence of antimicrobial resistance in the feed was examined in a case-control study...
2017: Porcine Health Management
Elizabeth Alvarado-Gomez, Mario Perez-Diaz, Donato Valdez-Perez, Jaime Ruiz-Garcia, Martin Magaña-Aquino, Gabriel Martinez-Castañon, Fidel Martinez-Gutierrez
A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Elodie Lefebvre, Pierre Lembre, Julien Picard, Lamia El-Guermah, Damien Seyer, Véronique Larreta Garde
Chronic wound colonization by bacterial biofilms is common and can cause various complications. An anti-biofilm strategy was developed around the co-entrapment of a commercially available antiseptic, PHMB (polyhexamethylene biguanide 4mgmL(-1)), with EDTA (Ethylen diamine tetra acetic acid, 20mM) in a gelatin gel. The two active compounds act synergistically against bacterial biofilms, but their efficiency is strongly reduced (16-fold) when entrapped inside the 5% gelatin gel, and they weaken the mechanical properties (50-fold) of the gel...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
R Lagrafeuille, S Miquel, D Balestrino, M Vareille-Delarbre, F Chain, P Langella, C Forestier
Beneficial bacteria represent potential sources of therapy, particularly in the battle against antibiotic-resistant pathogens. The Gram-negative bacillus Klebsiella pneumoniae is not only a paradigm of multi-resistant opportunistic pathogen, but it is also able to colonise the human intestine and displays a high capacity to form biofilm. In this study, the anti-biofilm activity of 140 neutralised Lactobacillus supernatants was assessed against K. pneumoniae. Among the 13 strains whose supernatant significantly impaired biofilm formation, Lactobacillus plantarum CIRM653 was selected because it was also able to impair K...
October 12, 2017: Beneficial Microbes
Han-Yu Chen, Yong-De Liu, Bin Dong
An anaerobic/aerobic moving-bed biofilm (A/O-MBBR) reactor system was constructed, and the treatment efficiency of aqueous antibiotics in wastewater was investigated. The effects of antibiotics on the microbial communities in the A/O-MBBR were also investigated. Under the optimized reaction conditions, removal of tetracycline antibiotics (TCs) was studied in a series of experiments. When a low concentration of tetracycline (TC) was added to the reactor system, high removal efficiency of conventional pollutants (TC concentration decreased from 10 turn to 2...
October 10, 2017: Bioprocess and Biosystems Engineering
Stephanie A Fong, Amanda Drilling, Sandra Morales, Marjolein E Cornet, Bradford A Woodworth, Wytske J Fokkens, Alkis J Psaltis, Sarah Vreugde, Peter-John Wormald
Introduction:Pseudomonas aeruginosa infections are prevalent amongst chronic rhinosinusitis (CRS) sufferers. Many P. aeruginosa strains form biofilms, leading to treatment failure. Lytic bacteriophages (phages) are viruses that infect, replicate within, and lyse bacteria, causing bacterial death. Aim: To assess the activity of a phage cocktail in eradicating biofilms of ex vivo P.aeruginosa isolates from CRS patients. Methods: P. aeruginosa isolates from CRS patients with and without cystic fibrosis (CF) across three continents were multi-locus sequence typed and tested for antibiotic resistance...
2017: Frontiers in Cellular and Infection Microbiology
Yulong Tan, Matthias Leonhard, Berit Schneider-Stickler
Silicone is frequently used in clinical and medical fields for medical devices. Mixed biofilms composed of Candida and bacterial species causes frequently failure of medical silicone devices, In this in vitro study, we analyzed mixed biofilm formation of clinically isolated non-albicans Candida species and Staphylococcus epidermidis, including Candida tropicalis, Candida krusei and Candida parapsilosis under the influence of different growth media (RPMI 1640, BHI and TSB) and several culture variables (incubation period, feeding conditions and FBS)...
October 5, 2017: Microbial Pathogenesis
Marcin Zawrotniak, Oliwia Bochenska, Justyna Karkowska-Kuleta, Karolina Seweryn-Ozog, Wataru Aoki, Mitsuyoshi Ueda, Andrzej Kozik, Maria Rapala-Kozik
Neutrophils use different mechanisms to cope with pathogens that invade the host organism. The most intriguing of these responses is a release of neutrophil extracellular traps (NETs) composed of decondensed chromatin and granular proteins with antimicrobial activity. An important potential target of NETs is Candida albicans-an opportunistic fungal pathogen that employs morphological and phenotype switches and biofilm formation during contact with neutrophils, accompanied by changes in epitope exposition that mask the pathogen from host recognition...
2017: Frontiers in Cellular and Infection Microbiology
Eman Jameel Fatani, Hamed H Almutairi, Ali O Alharbi, Yasser Obaidallah Alnakhli, Darshan Devang Divakar, Muzaheed, Abdulaziz Abdullah Alkheraif, Aftab Ahmed Khan
Orthodontic brackets made from stainless steel were introduced in dentistry, though they have less ability in reducing enamel demineralization and are not successful in preventing microbial as well as biofilm growth. In this study, we evaluated the significant role of different brackets in reducing enamel demineralization indirectly. Results from different tests indicate the significant reduction in adhesion, biofilm formation and slow growth of tested bacterial species on brackets coated with Ag + TiO2 and found to be statistically significant lower than control...
September 29, 2017: Microbial Pathogenesis
I L Maslennikova, M V Kuznetsova, I V Nekrasova, S V Shirshev
Pseudomonas aeruginosa (PA) responsible for acute and chronic infections often forms a well-organized bacterial population with different microbial species including commensal strains of Escherichia coli. Bacterial extracellular components of mixed culture can modulate the influence of bacteria on the neutrophil functions. The objective of this study was to compare the effect of pyocyanin, pyoverdine, LPS, exopolysaccharide of single species and mixed culture supernatants of PA strains and E. coli K12 on microbicidal, secretory activity of human neutrophils in vitro...
August 31, 2017: Pathogens and Disease
Carmen Solana, Matilde Ruiz-Linares, Pilar Baca, Mariano Jose Valderrama, Maria Teresa Arias-Moliz, Carmen Maria Ferrer-Luque
INTRODUCTION: The aim of this study was to determine the antimicrobial activity of mixed alkaline tetrasodium EDTA (EDTANa4)/sodium hypochlorite (NaOCl) solutions with and without the addition of cetrimide (CTR) against Enterococcus faecalis biofilms. METHODS: The antimicrobial solutions were evaluated on a 3-week biofilm of E. faecalis grown on radicular dentin blocks. The irrigating solutions were 2.5% NaOCl group, 20% EDTANa4 group, 10% EDTANa4 group, 2.5% NaOCl/10% EDTANa4 group, 2...
September 26, 2017: Journal of Endodontics
Erik Trampe, Richard W Castenholz, Jens E Larsen, Michael Kühl
Marine tufa-columns, formed by the hydrated carbonate mineral ikaite, present a unique alkaline microbial habitat only found in Ikka Fjord (SW-Greenland). The outermost parts of the ikaite columns exhibit a multitude of physico-chemical gradients, and the porous ikaite is colonized by endolithic phototrophic biofilms serving as a substrate for grazing epifauna, where scraping by sea urchins affects overall column-topography. We present a detailed study of the optical microenvironment, spatial organization, and photosynthetic activity of endolithic phototrophs within the porous ikaite crystal matrix...
September 26, 2017: Environmental Microbiology
Midian C Castillo Pedraza, Tatiana F Novais, Roberta C Faustoferri, Robert G Quivey, Anton Terekhov, Bruce R Hamaker, Marlise I Klein
Streptococcus mutans-derived exopolysaccharides are virulence determinants in the matrix of biofilms that cause caries. Extracellular DNA (eDNA) and lipoteichoic acid (LTA) are found in cariogenic biofilms, but their functions are unclear. Therefore, strains of S. mutans carrying single deletions that would modulate matrix components were used: eDNA - ∆lytS and ∆lytT; LTA - ∆dltA and ∆dltD; and insoluble exopolysaccharide - ΔgtfB. Single-species (parental strain S. mutans UA159 or individual mutant strains) and mixed-species (UA159 or mutant strain, Actinomyces naeslundii and Streptococcus gordonii) biofilms were evaluated...
September 25, 2017: Biofouling
Xianbin Ying, Kun Guo, Wei Chen, Yuan Gu, Dongsheng Shen, Yuyang Zhou, Yuxiang Liang, Yanfeng Wang, Meizhen Wang, Huajun Feng
Both anode potentials and substrates can affect the process of biofilm formation in bioelectrochemical systems, but it is unclear who primarily determine the anode-respiring bacteria (ARB) community structure and composition. To address this issue, we divided microbial electrolysis cells (MECs) into groups, feeding them with different substrates and culturing them at various potentials. Non-turnover cyclic voltammetry indicated that the extracellular electron transfer components were uniform when feeding acetate, because the same oxidation peaks occurred at - 0...
September 25, 2017: Applied Microbiology and Biotechnology
Waheed Miran, Jiseon Jang, Mohsin Nawaz, Asif Shahzad, Sang Eun Jeong, Che Ok Jeon, Dae Sung Lee
Microbial fuel cells (MFCs) have been widely investigated for organic-based waste/substrate conversion to electricity. However, toxic compounds such as heavy metals are ubiquitous in organic waste and wastewater. In this work, a sulfate reducing bacteria (SRB)-enriched anode is used to study the impact of Cu(2+) on MFC performance. This study demonstrates that MFC performance is slightly enhanced at concentrations of up to 20 mg/L of Cu(2+), owing to the stimulating effect of metals on biological reactions...
September 13, 2017: Chemosphere
Parthiban Anburajan, Arivalagan Pugazhendhi, Jong-Hun Park, Periyasamy Sivagurunathan, Gopalakrishnan Kumar, Sang-Hyoun Kim
This study investigated the effect of 5-hydroxymethylfurfural (5-HMF) on high-rate continuous fermentative H2 production in a lab-scale fixed bed reactor (FBR) inoculated with mixed culture granules and fed with 15g/L galactose at a hydraulic retention time of 6h and at 37°C. During the 83days of operation, 5-HMF up to 2.4g/L was spiked into the feedstock. The maximum hydrogen production performance of 26.6L/L-d and 2.9mol H2/mol galactoseadded were achieved at 5-HMF concentration of 0.6g/L. 5-HMF concentration exceeding 0...
September 5, 2017: Bioresource Technology
Yoshihito Naito, Hiromichi Yumoto, Kiran Kumar Hs, Takashi Matsuo, Katsuhiko Hirota, Yoichiro Miyake, Kan Nagao, Yoritoki Tomotake, Ryo Jimbo, Tetsuo Ichikawa
PURPOSE: To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. MATERIALS AND METHODS: Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus-mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24-well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days...
September 13, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
Rodrigo A Giacaman
The traditional concept of caries as a multifactorial transmittable and infectious disease has been challenged. Novel conceptual ideas have come to add to the complexity of this highly prevalent disease worldwide. Current etiological understanding of the disease has emphasized the pivotal role of sugar in caries. In fact, current definition points towards an ecological disease caused by the commensal microbiota that under ecological imbalances, mainly due to high and or frequent sugar consumption, creates a state of dysbiosis in the oral biofilm...
September 12, 2017: Oral Diseases
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