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https://www.readbyqxmd.com/read/29457734/role-of-nutrient-limitation-in-the-competition-between-uropathogenic-strains-of-klebsiella-pneumoniae-and-escherichia-coli-in-mixed-biofilms
#1
Guillermo E Juarez, Estela M Galván
Klebsiella pneumoniae and Escherichia coli form mixed species biofilms in catheter-associated urinary tract infections. Recently, a detrimental effect of K. pneumoniae over E. coli was observed in mixed species biofilms grown in an artificial urine medium. The mechanism behind this competitive interaction was studied. K. pneumoniae partially outcompeted E. coli in early-stage batch-fed biofilms, whereas both microorganisms co-exist at longer times (K. pneumoniae:E. coli ratio, 55:1), as shown by cell counts and confocal microscopy...
February 19, 2018: Biofouling
https://www.readbyqxmd.com/read/29451932/in-vitro-screening-of-the-antibacterial-and-anti-candida-properties-of-crushed-nonantimicrobial-drugs-frequently-prescribed-in-nursing-homes
#2
Julie Lamure, Marlène Chevalier, Pascal Rathelot, Françoise Mignolet, Isabelle Precheur
Frail older adults often experience swallowing disorders, prompting nursing staff to crush tablets, open capsules, and mix drugs into their meals or gelled water. However, crushing drugs can lead to pharmacological and gustatory problems. As crushed drugs can stay in prolonged contact with oral microbial biofilm, the current study aimed to investigate their antimicrobial properties. Crushed drugs were diluted in 1 mL of isotonic water and assayed in vitro for: (a) growth inhibition of five bacterial strains and Candida albicans by the diffusion method; (b) inhibition of Streptococcus salivarius and C...
February 13, 2018: Research in Gerontological Nursing
https://www.readbyqxmd.com/read/29430962/effect-of-a-mouthrinse-containing-malva-sylvestris-on-the-viability-and-activity-of-microcosm-biofilm-and-on-enamel-demineralization-compared-to-known-antimicrobials-mouthrinses
#3
Aline Silva Braga, Juliana Gonçalves Pires, Ana Carolina Magalhães
The purpose of this study was to evaluate the antimicrobial (anti-biofilm) and anti-caries (enamel demineralization prevention) effects of Malva sylvestris (Malvatricin® Plus) compared with known antimicrobial mouthrinses. Microcosm biofilm was produced on enamel, using inoculum from pooled human saliva mixed with McBain saliva (0.2% sucrose) for 14 days. The biofilm was treated with mouthrinses for 1 min day-1. Oral-B® Complete, Listerine® Zero and Malvatricin® Plus had the greatest effect on the reduction of biofilm viability (p < 0...
February 11, 2018: Biofouling
https://www.readbyqxmd.com/read/29423635/damage-to-offshore-production-facilities-by-corrosive-microbial-biofilms
#4
REVIEW
Adrien Vigneron, Ian M Head, Nicolas Tsesmetzis
In offshore production facilities, large amounts of deaerated seawater are continuously injected to maintain pressure in oil reservoirs and equivalent volumes of fluids, composed of an oil/gas, and water mixture are produced. This process, brewing billions of liters of biphasic fluids particularly rich in microorganisms, goes through complex steel pipeline networks that are particularly prone to biofilm formation. Consequently, offshore facilities are frequently victims of severe microbiologically influenced corrosion...
February 8, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29410771/inhibition-of-steptococcus-mutans-biofilm-formation-by-extracts-of-tenacibaculum-sp-20j-a-bacterium-with-wide-spectrum-quorum-quenching-activity
#5
Andrea Muras, Celia Mayer, Manuel Romero, Tamara Camino, Maria D Ferrer, Alex Mira, Ana Otero
Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method...
2018: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/29401743/current-and-future-applications-of-biomedical-engineering-for-proteomic-profiling-predictive-biomarkers-in-neuro-traumatology
#6
REVIEW
Mario Ganau, Nikolaos Syrmos, Marco Paris, Laura Ganau, Gianfranco K I Ligarotti, Ali Moghaddamjou, Salvatore Chibbaro, Andrea Soddu, Rossano Ambu, Lara Prisco
This systematic review aims to summarize the impact of nanotechnology and biomedical engineering in defining clinically meaningful predictive biomarkers in patients with traumatic brain injury (TBI), a critical worldwide health problem with an estimated 10 billion people affected annually worldwide. Data were collected through a review of the existing English literature performed on Scopus, MEDLINE, MEDLINE in Process, EMBASE, and/or Cochrane Central Register of Controlled Trials. Only experimental articles revolving around the management of TBI, in which the role of new devices based on innovative discoveries coming from the field of nanotechnology and biomedical engineering were highlighted, have been included and analyzed in this study...
February 5, 2018: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29401728/antibacterial-effects-of-chitosan-cationic-peptide-nanoparticles
#7
Frans Ricardo Tamara, Chi Lin, Fwu-Long Mi, Yi-Cheng Ho
This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and antibacterial activities of the hybrid nanoparticles were investigated. The antibacterial experiments demonstrated that the addition of protamine (125 µg/mL) in the hybrid nanoparticles (500 µg/mL chitosan and 166...
February 5, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29398539/synthesis-kinetics-and-inhibition-of-escherichia-coli-heptosyltransferase-i-by-monosaccharide-analogues-of-lipid-a
#8
Noreen K Nkosana, Daniel J Czyzyk, Zarek S Siegel, Joy M Cote, Erika A Taylor
Gram-negative bacteria comprise the majority of microbes that cause infections that are resistant to pre-existing antibiotics. The complex cell wall architecture contributes to their ability to form biofilms, which are often implicated in hospital-acquired infections. Biofilms promote antibiotic resistance by enabling the bacteria to survive hostile environments such as UV radiation, pH shifts, and antibiotics. The outer membrane of Gram-negative bacteria contains lipopolysaccharide (LPS), which plays a role in adhesion to surfaces and formation of biofilms...
February 2, 2018: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29397617/-the-research-of-the-innate-defense-regulator-peptide-on-the-effects-of-methicillin-resistant-staphylococcus-aureus-biofilm
#9
X Shi, Y X Qin, X Y Wan
Objective: To investigate the destruction of the mature biofilm and the inhibitory effect of the biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) by different concentrations of the innate defense regulatory peptide (IDR-1018). Methods: 1 ×10(5)CFU /ml MRSA was inoculated uniformly into 96 well plates, the biofilm model would be completed after 48 h. Given the different concentration of IDR-1018 solution as the experimental group double diluted with tryptic soy broth (TSB), the concentration in bacteria suspension reached 3...
January 23, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29368511/peptidoglycan-compositional-analysis-of-enterococcus-faecalis-biofilm-by-stable-isotope-labeling-by-amino-acids-in-bacterial-culture
#10
James D Chang, Ashley G Wallace, Erin E Foster, Sung Joon Kim
Peptidoglycan (PG) is a major component of the cell wall in Enterococcus faecalis. Accurate analysis of PG composition provides crucial insights into bacteria's cellular functions and responses to external stimuli, but this analysis remains challenging due to various chemical modifications to PG-repeat subunits. We characterized changes to the PG composition in E. faecalis grown as planktonic bacteria and biofilm by developing "Stable Isotope Labeling by Amino Acids in Bacterial Culture" (SILAB) optimized for bacterial cultures with incomplete amino acid labeling...
January 25, 2018: Biochemistry
https://www.readbyqxmd.com/read/29357385/infectious-blood-source-alters-early-foregut-infection-and-regurgitative-transmission-of-yersinia-pestis-by-rodent-fleas
#11
David M Bland, Clayton O Jarrett, Christopher F Bosio, B Joseph Hinnebusch
Fleas can transmit Yersinia pestis by two mechanisms, early-phase transmission (EPT) and biofilm-dependent transmission (BDT). Transmission efficiency varies among flea species and the results from different studies have not always been consistent. One complicating variable is the species of rodent blood used for the infectious blood meal. To gain insight into the mechanism of EPT and the effect that host blood has on it, fleas were fed bacteremic mouse, rat, guinea pig, or gerbil blood; and the location and characteristics of the infection in the digestive tract and transmissibility of Y...
January 22, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29346268/effect-of-novel-quercetin-titanium-dioxide-decorated-multi-walled-carbon-nanotubes-nanocomposite-on-bacillus-subtilis-biofilm-development
#12
Diana S Raie, Eisha Mhatre, Doaa S El-Desouki, Ahmed Labena, Gamal El-Ghannam, Laila A Farahat, Tareq Youssef, Wolfgang Fritzsche, Ákos T Kovács
The present work was targeted to design a surface against cell seeding and adhering of bacteria, Bacillus subtilis. A multi-walled carbon nanotube/titanium dioxide nano-power was produced via simple mixing of carbon nanotube and titanium dioxide nanoparticles during the sol-gel process followed by heat treatment. Successfully, quercetin was immobilized on the nanocomposite via physical adsorption to form a quercetin/multi-walled carbon nanotube/titanium dioxide nanocomposite. The adhesion of bacteria on the coated-slides was verified after 24 h using confocal laser-scanning microscopy...
January 18, 2018: Materials
https://www.readbyqxmd.com/read/29342260/interaction-between-staphylococcus-aureus-and-pseudomonas-aeruginosa-is-beneficial-for-colonisation-and-pathogenicity-in-a-mixed-biofilm
#13
Patrícia M Alves, Eide Al-Badi, Cathryn Withycombe, Paul M Jones, Kevin J Purdy, Sarah E Maddocks
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early ( < 24h) biofilm formation, and interaction with human keratinocytes...
January 12, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29339624/the-combined-effects-of-carbon-nitrogen-ratio-suspended-biomass-hydraulic-retention-time-and-dissolved-oxygen-on-nutrient-removal-in-a-laboratory-scale-anaerobic-anoxic-oxic-activated-sludge-biofilm-reactor
#14
D S Manu, Arun Kumar Thalla
The current trend in sustainable development deals mainly with environmental management. There is a need for economically affordable, advanced treatment methods for the proper treatment and management of domestic wastewater containing excess nutrients (such as nitrogen and phosphorus) which can cause eutrophication. The reduction of the excess nutrient content of wastewater by appropriate technology is of much concern to the environmentalist. In the current study, a novel integrated anaerobic-anoxic-oxic activated sludge biofilm (A2O-AS-biofilm) reactor was designed and operated to improve the biological nutrient removal by varying reactor operating conditions such as carbon to nitrogen (C/N) ratio, suspended biomass, hydraulic retention time (HRT) and dissolved oxygen (DO)...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29334812/anti-biofilm-effect-of-a-butenolide-polymer-coating-and-metatranscriptomic-analyses
#15
Wei Ding, Chunfeng Ma, Weipeng Zhang, Hoyin Chiang, Chunkit Tam, Ying Xu, Guangzhao Zhang, Pei-Yuan Qian
Butenolide is an environmentally friendly antifouling natural product, but its efficiency and mechanism in preventing biofilm formation have not been examined. Furthermore, controlling the release of butenolide from paints into seawater is technically challenging. A coating was developed by mixing butenolide with a biodegradable polymer, poly (ε-caprolactone)-based polyurethane, and a one-month in situ anti-biofilm test was conducted in a subtidal area. The constant release of butenolide from the surface suggested that its release was well controlled...
January 2018: Biofouling
https://www.readbyqxmd.com/read/29330184/microbiomes-in-dishwashers-analysis-of-the-microbial-diversity-and-putative-opportunistic-pathogens-in-dishwasher-biofilm-communities
#16
Prem Krishnan Raghupathi, Jerneja Zupančič, Asker Daniel Brejnrod, Samuel Jacquiod, Kurt Houf, Mette Burmølle, Nina Gunde-Cimerman, Søren J Sørensen
Extreme habitats are not only limited to natural environments, but also apply to man-made systems, for instance household appliances such as dishwashers. Limiting factors, such as high temperatures, high and low pH, high NaCl concentrations, presence of detergents and shear force from water during washing cycles define the microbial survival in this extreme system. Fungal and bacterial diversity in biofilms isolated from rubber seals of 24 different household dishwashers were investigated using next generation sequencing...
January 12, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29316463/electrochemical-and-microbial-community-responses-of-electrochemically-active-biofilms-to-copper-ions-in-bioelectrochemical-systems
#17
Yaping Zhang, Guanqun Li, Jing Wen, Yangao Xu, Jian Sun, Xun-An Ning, Xingwen Lu, Yujie Wang, Zuoyi Yang, Yong Yuan
Heavy metals play an important role in the conductivity of solution, power generation and activity of microorganisms in bioelectrochemical systems (BESs). However, effect of heavy metal on the process of exoelectrogenesis metabolism and extracellular electron transfer of electrochemically active biofilms (EABs) was poorly understood. Herein, we investigated the impact of Cu2+ at gradually increasing concentration on the morphological and electrochemical performance and bacterial communities of anodic biofilms in mixed-culture BESs...
January 5, 2018: Chemosphere
https://www.readbyqxmd.com/read/29288452/application-of-rna-seq-and-bioimaging-methods-to-study-microbe-microbe-interactions-and-their-effects-on-biofilm-formation-and-gene-expression
#18
Cristina Isabel Amador, Claus Sternberg, Lars Jelsbak
Complex interactions between pathogenic bacteria, the microbiota, and the host can modify pathogen physiology and behavior. We describe two different experimental approaches to study microbe-microbe interactions in in vitro systems containing surface-associated microbial populations. One method is the application of RNA sequencing (RNA-seq) to determine the transcriptional changes in pathogenic bacteria in response to microbial interspecies interactions. The other method combines flow cell devices for bacterial cultivation and growth with high-resolution bioimaging to analyze the microscale structural organization of interacting microbial populations within mixed-species biofilms...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29284195/extracellular-matrix-influence-in-streptococcus-mutans-gene-expression-in-a-cariogenic-biofilm
#19
Elkin Jahir Florez Salamanca, Marlise Inêz Klein
Caries etiology is biofilm-diet dependent. Biofilms are highly dynamic and structured microbial communities enmeshed in a three-dimensional extracellular matrix. The study evaluated the expression dynamics of Streptococcus mutans genes associated with exopolysaccharides (EPS) (gtfBCD, gbpB, dexA), lipoteichoic acids (LTA) (dltABCD, SMU_775c) and extracellular DNA (eDNA) (lytST, lrgAB, ccpA) during matrix development within a mixed-species biofilm of S. mutans, Actinomyces naeslundii and Streptococcus gordonii...
December 28, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/29279769/biofilm-formation-on-denture-base-resin-including-zno-cao-and-tio2-nanoparticles
#20
Melissa Anwander, Martin Rosentritt, Sibylle Schneider-Feyrer, Sebastian Hahnel
PURPOSE: This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and TiO2 on biofilm formation. MATERIALS AND METHODS: Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and TiO2 nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens...
December 2017: Journal of Advanced Prosthodontics
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