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https://www.readbyqxmd.com/read/28325016/sleep-stage-classification-by-non-contact-vital-signs-indices-using-doppler-radar-sensors
#1
Masayuki Kagawa, Kazuki Suzumura, Takemi Matsui
Disturbed sleep has become more common in recent years. To improve the quality of sleep, undergoing sleep observation has gained interest as a means to resolve possible problems. In this paper, we evaluate a non-restrictive and non-contact method for classifying real-time sleep stages and report on its potential applications. The proposed system measures heart rate (HR), heart rate variability (HRV), body movements, and respiratory signals of a sleeping person using two 24-GHz microwave radars placed beneath the mattress...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324987/sleep-architecture-measurement-based-on-cardiorespiratory-parameters
#2
Alexander Tataraidze, Lyudmila Korostovtseva, Lesya Anishchenko, Mikhail Bochkarev, Yurii Sviryaev
This paper presents a method for the detection of wakeful state, rapid eye movement sleep (REM), light sleep (N1&N2) and deep sleep (N3&N4) based on cardiorespiratory parameters. Experiments were conducted with data of 625 subjects without sleep-disordered breathing selected from the SHHS dataset. Compared to previous studies, our method considers results of neighboring epochs classification and epoch position over record time. The method demonstrates Cohen's kappa of 0.57 ± 0.13 and the accuracy of 71.4 ± 8...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324964/gastric-mucosa-injury-quantification-in-an-ischemia-reperfusion-experimental-model
#3
E Pena-Mercado, M Garcia-Lorenzana, E Arechaga, C H Gonzalez-de la Rosa, N E Beltran
Gastric ischemia - reperfusion (I/R) injury is an important clinical problem, which is developed in more than 80% of critically ill patients. I/R is caused by interruption of blood supply to an organ or tissue followed by blood reflow into the exposed area, leading to multiple organ failure and death. Gastric reactance has been proposed to measure tissue injury caused by ischemia. The present study evaluates a new method to quantify gastric tissue damage due to I/R, and assess its relation to gastric reactance changes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324936/a-preliminary-study-on-multi-wavelength-ppg-based-pulse-transit-time-detection-for-cuffless-blood-pressure-measurement
#4
Jing Liu, Yuan-Ting Zhang, Xiao-Rong Ding, Wen-Xuan Dai, Ni Zhao
Pulse transit time (PTT) has been widely studied as an index of blood pressure (BP) changes. In recent years, some prototypes of PTT-based wearable BP measurement devices have been developed, which can relieve users from the discomfort caused by the inflating cuff used in auscultatory and oscillometric BP measurement techniques. However, in the common practice for PTT detection, multi-site sensor implementation on human body is required, making it difficult for the integration of wearable devices. Since multi-wavelength (MW) photoplethysmogram (PPG) signals carry blood pulsation information of different blood vessels embedded in different skin depths, the time difference between different wavelength PPG signals collected at the same body site can be treated as a special PTT on a short length of blood vessels beneath the skin...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324874/strategies-and-outcomes-of-repeat-mitral-valve-interventions-after-failed-mitraclip-therapy
#5
Carmelo Grasso, Sergio Buccheri, Davide Capodanno, Antonio Popolo Rubbio, Maria Elena Di Salvo, Salvatore Scandura, Sarah Mangiafico, Tatiana Salerno, Stefano Cannata, Veronica Dezio, Giuseppe Castania, Marco Barbanti, Piera Capranzano, Corrado Tamburino
Percutaneous mitral valve repair (PMVR) with the MitraClip system (Abbott Vascular, Santa Clara, CA, USA) is a valid therapeutic option for patients with severe mitral regurgitation (MR) deemed to be at high or prohibitive surgical risk. Despite the reassuring data on efficacy and long-term durability of the procedure, the proportion of patients with residual or relapsing severe MR after MitraClip therapy is not negligible. In light of the detrimental prognostic impact of severe MR, repeat interventions are increasingly performed in clinical practice using different techniques...
March 22, 2017: Cardiology
https://www.readbyqxmd.com/read/28324836/pahs-behavior-in-surface-water-and-groundwater-of-the-yellow-river-estuary-evidence-from-isotopes-and-hydrochemistry
#6
Jing Li, Fadong Li, Qiang Liu
Large-scale irrigation projects have impacted the regional surface-groundwater interactions in the North China Plain (NCP). Given this concern, the aim of this study is to evaluate levels of PAH pollution, identify the sources of the PAHs, analyze the influence of surface-groundwater interactions on PAH distribution, and propose urgent management strategies for PAHs in China's agricultural areas. PAH concentrations, hydrochemical indicators and stable isotopic compositions (δ(18)O and δ(2)H) were determined for surface water (SW) and groundwater (GW) samples...
March 18, 2017: Chemosphere
https://www.readbyqxmd.com/read/28324782/two-dimensional-bismuth-oxybromide-coupled-with-molybdenum-disulphide-for-enhanced-dye-degradation-using-low-power-energy-saving-light-bulb
#7
W P Cathie Lee, Fhu-Hin Wong, Nicole K Attenborough, Xin Ying Kong, Lling-Lling Tan, S Sumathi, Siang-Piao Chai
In the present work, two-dimensional bismuth oxybromide (BiOBr) was synthesized and coupled with co-catalyst molybdenum disulphide (MoS2) via a simple hydrothermal process. The photoactivity of the resulting hybrid photocatalyst (MoS2/BiOBr) was evaluated under the irradiation of 15 W energy-saving light bulb at ambient condition using Reactive Black 5 (RB5) as model dye solution. The photo-degradation of RB5 by BiOBr loaded with 0.2 wt% MoS2 (MoBi-2) exhibited more than 1.4 and 5.0 folds of enhancement over pristine BiOBr and titanium dioxide (Degussa, P25), respectively...
March 18, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28324777/a-feasibility-study-of-a-novel-spectral-method-using-radiofrequency-ultrasound-data-for-monitoring-laser-tissue-ablation
#8
Enrico Vannacci, Simona Granchi, Luca Breschi, Elena Biagi
This paper presents preliminary results of a new non-invasive ultrasound monitoring method called TUV (Thermotherapy Ultrasonic View) able to investigate structural tissue modifications caused by minimally invasive percutaneous laser ablation. The method, based on the spectral analysis of the raw ultrasound radiofrequency signal, develops spectral parameters in a multidimensional space and its N dimensions are represented by the central frequencies of the sub bands the signal spectrum is decomposed into. Signal processing has been performed on the data related to 7 laser treatments performed on 4 samples of removed prostatic glands which underwent laser ablation at power of 3W, 4W and 5W and energy of 1800J...
March 6, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28324762/chrysanthemum-morphology-photosynthetic-efficiency-and-antioxidant-capacity-are-differentially-modified-by-light-quality
#9
Liang Zheng, Marie-Christine Van Labeke
The effect of light quality on leaf morphology, photosynthetic efficiency and antioxidant capacity of leaves that fully developed under a specific spectrum was investigated in Chrysanthemum cv. Four light treatments were applied at 100μmolm(-2)s(-1) and a photoperiod of 14h using light-emitting diodes, which were 100% red (R), 100% blue (B), 75% red with 25% blue (RB) and white (W), respectively. Intraspecific variation was investigated by studying the response of eight cultivars. Overall, red light significantly decreased the leaf area while the thinnest leaves were observed for W...
March 11, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28324740/evolution-fruit-fly-clocks-on-the-edge
#10
Charalambos P Kyriacou
Fruit flies evolved in tropical regions under stable light-dark cycles. However, their photosensitive circadian clock had to adapt to extreme seasonal photoperiods during their colonisation of temperate regions. This was achieved by changing the neuronal expression of two key clock-related components.
March 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28324723/specific-light-up-pullulan-based-nanoparticles-with-reduction-triggered-emission-and-activatable-photoactivity-for-the-imaging-and-photodynamic-killing-of-cancer-cells
#11
Jing Xia, Liuwei Zhang, Ming Qian, Yongming Bao, Jingyun Wang, Yachen Li
Activatable photosensitizers that can be activated by cancer-associated stimuli have drawn increasing attention for simultaneous fluorescence imaging and photodynamic ablation of cancer cells. Here, we developed a cancer-cell specific photosensitizer nano-delivery system by synthesizing protoporphyrin IX (PpIX)-conjugated pullulan (P) with reducible disulfide bonds. The amphiphilic P-s-s-PpIX conjugate self-assembled in aqueous condition to form core-shell structured nanoparticles (P-s-s-PpIX NPs) with average size of 166nm, showing reduction-controllable stability...
March 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28324702/the-cag-polymorphism-in-androgen-receptor-ar-gene-impacts-the-moral-permissibility-of-harmful-behavior-in-females
#12
Pingyuan Gong, Pengpeng Fang, Xing Yang, Wenzhao Ru, Bei Wang, Xiaocai Gao, Jinting Liu
The moral permissibility of harm is strikingly varied among individuals. In light of the connection between testosterone levels and utilitarian moral judgment, this study examined to what extent a CAG polymorphism in the androgen receptor gene, a genetic polymorphism with the ability to regulate testosterone function, contributes to individual differences in moral judgment. Four hundred and thirty-nine Chinese Han participants completed permissibility ratings of harm in moral dilemmas and moral transgression scenarios...
March 7, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28324655/tracking-the-energy-flow-on-nanoscale-via-sample-transmitted-excitation-photoluminescence-spectroscopy
#13
Pavel Moroz, Natalia Razgoniaeva, Yufan He, Gregory Jensen, Holly Eckard, H Peter Lu, Mikhail Zamkov
Tracking the energy flow in nanoscale materials is an important yet challenging goal. Experimental methods for probing the intermolecular energy transfer (ET) are often burdened by the spectral crosstalk between donor and acceptor species, which complicates unraveling their individual contributions. This issue is particularly prominent in inorganic nanoparticles and biological macromolecules featuring broad absorbing profiles. Here, we demonstrate a general spectroscopic strategy for measuring the energy transfer efficiency between nanostructured or molecular dyes exhibiting a significant donor-acceptor spectral overlap...
March 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28324654/a-general-strategy-for-visible-light-decaging-based-on-the-quinone-trimethyl-lock
#14
David P Walton, Dennis A Dougherty
Visible-light triggered quinone trimethyl locks are reported as a general design for long-wavelength photoremovable protecting groups for alcohols and amines. Intramolecular photoreduction unmasks a reactive phenol that undergoes fast lactonization to release alcohols and amines. Model substrates are released in quantitative yield along with well-defined, colorless hydroquinone byproducts. Substituent modifications of the quinone core allow absorption from 400-600 nm.
March 21, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28324610/designing-image-analysis-pipelines-in-light-microscopy-a-rational-approach
#15
Ignacio Arganda-Carreras, Philippe Andrey
With the progress of microscopy techniques and the rapidly growing amounts of acquired imaging data, there is an increased need for automated image processing and analysis solutions in biological studies. Each new application requires the design of a specific image analysis pipeline, by assembling a series of image processing operations. Many commercial or free bioimage analysis software are now available and several textbooks and reviews have presented the mathematical and computational fundamentals of image processing and analysis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324607/sted-imaging-in-drosophila-brain-slices
#16
Sandra Fendl, Jesús Pujol-Martí, Joel Ryan, Alexander Borst, Robert Kasper
Super-resolution microscopy is a very powerful tool to investigate fine cellular structures and molecular arrangements in biological systems. For instance, stimulated emission depletion (STED) microscopy has been successfully used in recent years to investigate the arrangement and colocalization of different protein species in cells in culture and on the surface of specimens. However, because of its extreme sensitivity to light scattering, super-resolution imaging deep inside tissues remains a challenge. Here, we describe the preparation of thin slices from the fruit fly (Drosophila melanogaster) brain, subsequent immunolabeling and imaging with STED microscopy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324606/sample-preparation-and-choice-of-fluorophores-for-single-and-dual-color-photo-activated-localization-microscopy-palm-with-bacterial-cells
#17
Juri N Bach, Giacomo Giacomelli, Marc Bramkamp
Photo-activated localization microscopy (PALM) is one of the light microscopy techniques providing highest resolution. Single photo-activatable or photo-switchable fluorescent molecules are stochastically excited. The point spread function of this event is recorded and the exact fluorophore position is calculated. This chapter describes how bacterial samples can be prepared for PALM to achieve routinely a resolution of ≤30 nm using fluorophores such as mNeonGreen, Dendra2, and PAmCherry. It is also explained how to perform multicolor PALM and combine it with total internal reflection (TIRF) microscopy to increase resolution...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324600/light-sheet-fluorescence-microscopy-chemical-clearing-and-labeling-protocols-for-ultramicroscopy
#18
Nina Jährling, Klaus Becker, Saiedeh Saghafi, Hans-Ulrich Dodt
Light-sheet microscopy is an effective technique in neuroscience, developmental biology, and cancer research for visualizing and analyzing cellular networks and whole organs in three dimensions. Because this technique requires specimens to be translucent they commonly have to be cleared before microscopy inspection. Here, we provide 3DISCO based protocols for preparing cleared samples of immuno-stained neural networks, lectin-labeled vascular networks, and Methoxy-X04 labeled beta-amyloid plaques in mice. 3DISCO utilizes the lipophilic solvents tetrahydrofuran (THF) and dibenzylether (DBE) for dehydration and successive clearing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324599/three-dimensional-live-imaging-of-filamentous-fungi-with-light-sheet-based-fluorescence-microscopy-lsfm
#19
Francesco Pampaloni, Laura Knuppertz, Andrea Hamann, Heinz D Osiewacz, Ernst H K Stelzer
We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28324598/introduction-to-modern-methods-in-light-microscopy
#20
Joel Ryan, Abby R Gerhold, Vincent Boudreau, Lydia Smith, Paul S Maddox
For centuries, light microscopy has been a key method in biological research, from the early work of Robert Hooke describing biological organisms as cells, to the latest in live-cell and single-molecule systems. Here, we introduce some of the key concepts related to the development and implementation of modern microscopy techniques. We briefly discuss the basics of optics in the microscope, super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy...
2017: Methods in Molecular Biology
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