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https://www.readbyqxmd.com/read/28446016/biodegradation-and-biocompatibility-of-poly-l-lactic-acid-implantable-mesh
#1
Sang-Don Yoon, Young-Sam Kwon, Kyu-Sung Lee
PURPOSE: The purpose of this study was to evaluate the biodegradation and biocompatibility of poly L-lactic acid (PLLA) implantable mesh under in vitro and in vivo conditions. METHODS: PLLA mesh was examined for changes in weight and tensile strength. The histology of the tissue around the PLLA implant was also evaluated. RESULTS: The weight and tensile strength of the PLLA prosthesis was stable for 180 days. In addition, the surface of the PLLA mesh was not digested under in vitro or in vivo conditions as determined by scanning electron microscope...
April 2017: International Neurourology Journal
https://www.readbyqxmd.com/read/28444600/antibiotic-sensitivity-pattern-of-indigenous-lactobacilli-isolated-from-curd-and-human-milk-samples
#2
Chetan Sharma, Sachin Gulati, Nishchal Thakur, Brij Pal Singh, Sanjolly Gupta, Simranpreet Kaur, Santosh Kumar Mishra, Anil Kumar Puniya, Jatinder Pal Singh Gill, Harsh Panwar
The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host. This study evaluated the antibiotic susceptibility patterns of LAB isolated from curd (20) and human milk (11) samples...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444596/response-surface-design-for-accumulation-of-selenium-by-different-lactic-acid-bacteria
#3
Jingpeng Yang, Yao Li, Li Zhang, Mingtao Fan, Xinyuan Wei
The accumulation of selenium (Se) by Lactobacillus delbrueckii ssp. bulgaricus (Lb) and Streptococcus thermophilus (St) at the different cultivation conditions, including initial pH, inoculum dose (%), and temperature (°C), was investigated in this work. Se enrichment efficiency was optimized using the Design-Expert software for response surface methodology on a basis of single-factor experiment. The antioxidant activities of Se-enriched Lactic acid bacteria (LAB) were also investigated. The qualitative analysis of Se-enriched LAB was performed by FT-IR spectra...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28443832/protective-buffering-capacity-of-restorative-dental-materials-in-vitro
#4
Markus Fuss, Michael J Wicht, Thomas Attin, Sonja H M Derman, Michael J Noack
PURPOSE: To investigate the buffering capacity of restorative materials during a simulated carious and intrinsic erosive attack. MATERIALS AND METHODS: Cavities with a volume of 130 μl were milled (Cerec MC XL) out of blocks of Ceram X Mono (CM), Quixfil (QX), Filtek Supreme (FS), Apa Fill 3 (AF), an experimental dual-curing composite containing a bioactive glass (EX), Dyract eXtra (DY), Beautifil (BE), Equia Fil (EQ), Telio CAD (TL) (negative control), TheraCal (TC; positive control), and extracted teeth (ED)...
April 25, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/28442158/diversity-and-evolutionary-aspects-of-mucin-binding-mucbp-domain-repeats-among-lactobacillus-plantarum-group-strains-through-comparative-genetic-analysis
#5
Sundru Manjulata Devi, Prakash M Halami
Mucin binding protein (Mub) of Lactobacillus plantarum- group (LPG) is considered as a potential marker gene due to its diversity and adherence property to host intestinal layer. We recently observed strain specific diversity in mub gene of LPG strains (n=8) and grouped them into Type I, II and III based on the insertion and/or deletion of MucBP domain repeats (Devi et al., 2016). The mub gene expression pattern noted by real-time PCR revealed 67.64 increased fold change in MTCC 9483 with four MucBP repeats...
April 12, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28439768/discrimination-of-wine-lactic-acid-bacteria-by-raman-spectroscopy
#6
Susan B Rodriguez, Mark A Thornton, Roy J Thornton
Species of Lactobacillus, Pediococcus, Oenococcus, and Leuconostoc play an important role in winemaking, as either inoculants or contaminants. The metabolic products of these lactic acid bacteria have considerable effects on the flavor, aroma, and texture of a wine. However, analysis of a wine's microflora, especially the bacteria, is rarely done unless spoilage becomes evident, and identification at the species or strain level is uncommon as the methods required are technically difficult and expensive. In this work, we used Raman spectral fingerprints to discriminate 19 strains of Lactobacillus, Pediococcus, and Oenococcus...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439679/screening-lactic-acid-bacteria-strains-with-ability-to-bind-di-n-butyl-phthalate-via-turbiscan-technique
#7
Zhao Lili, Zhao Hongfei, Sana Shoukat, Zhang Xiaochen, Zhang Bolin
Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant that poses a risk to humans. Previous work indicates that the ability of lactic acid bacteria (LAB) to bind phthalic acid esters is strain-specific. As cell suspensions of LAB strains in aqueous solution are likely to be colloidal dispersions, this study provided a technique to efficiently screen LAB strains that bind DBP via Turbiscan, which has been widely used to measure the stability of emulsions or colloidal dispersions. Eleven LAB strains belonging to Lactobacillus plantarum, Lb...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439489/detection-and-analysis-of-lactobacillus-paracasei-penicillin-binding-proteins-revealed-the-presence-of-cholate-sensitive-penicillin-binding-protein-3-and-an-elongated-cell-shape-in-a-cholate-sensitive-strain
#8
Masahiro Hattori, Glaezel Angelique Torres, Naoto Tanaka, Sanae Okada, Akihito Endo, Junichi Nakagawa
Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3...
2017: Bioscience of Microbiota, Food and Health
https://www.readbyqxmd.com/read/28439299/substance-p-enhances-lactic-acid-and-tyramine-production-in-enterococcus-faecalis-v583-and-promotes-its-cytotoxic-effect-on-intestinal-caco-2-tc7-cells
#9
Kelly Biaggini, Valérie Borrel, Sabine Szunerits, Rabah Boukherroub, Awa N'Diaye, Arthur Zébré, Maryse Bonnin-Jusserand, Guillaume Duflos, Marc Feuilloley, Djamel Drider, Pierre Déchelotte, Nathalie Connil
BACKGROUND: Enterococcus faecalis, generally considered as a saprophytic bowel commensal, has recently emerged as an important nosocomial pathogen causing severe urinary tract infections, surgical wound infections, bacteremia, and bacterial endocarditis. This bacterium is capable of forming biofilms on various surfaces and its high level of antibiotic resistance contributes to its pathogenicity. The aim of this study was to evaluate the effect on E. faecalis, of Substance P (SP), an antimicrobial peptide that is produced in the gut and skin...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28438697/predicting-drug-loading-in-pla-peg-nanoparticles
#10
M Meunier, A Goupil, P Lienard
Polymer nanoparticles present advantageous physical and biopharmaceutical properties as drug delivery systems compared to conventional liquid formulations. Active pharmaceutical ingredients (APIs) are often hydrophobic, thus not soluble in conventional liquid delivery. Encapsulating the drugs in polymer nanoparticles can improve their pharmacological and bio-distribution properties, preventing rapid clearance from the bloodstream. Such nanoparticles are commonly made of non-toxic amphiphilic self-assembling block copolymers where the core (poly-[D,L-lactic acid] or PLA) serves as a reservoir for the API and the external part (Poly-(Ethylene-Glycol) or PEG) serves as a stealth corona to avoid capture by macrophage...
April 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28438574/extreme-hypertriglyceridemia-pseudohyponatremia-and-pseudoacidosis-in-a-neonate-with-lipoprotein-lipase-deficiency-due-to-segmental-uniparental-disomy
#11
Ambika P Ashraf, Anna C E Hurst, Abhimanyu Garg
Extreme hypertriglyceridemia is rare in the neonatal period. We report a neonate with lipoprotein lipase (LPL) deficiency who presented with diagnostic and management conundrum. A full-term 36-day-old female was noted to have "Pepto-Bismol like" blood when repeating a newborn screening. The initial plasma triglyceride level was 24,318 mg/dL. The laboratory tests revealed serum bicarbonate level of <5 mmol/L, sodium of 127 mmol/L, and severe anemia. There were no signs of acute distress. The point of care capillary blood testing, however, demonstrated normal serum pH (7...
April 3, 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/28438266/physiological-role-of-two-component-signal-transduction-systems-in-food-associated-lactic-acid-bacteria
#12
Vicente Monedero, Ainhoa Revilla-Guarinos, Manuel Zúñiga
Two-component systems (TCSs) are widespread signal transduction pathways mainly found in bacteria where they play a major role in adaptation to changing environmental conditions. TCSs generally consist of sensor histidine kinases that autophosphorylate in response to a specific stimulus and subsequently transfer the phosphate group to their cognate response regulators thus modulating their activity, usually as transcriptional regulators. In this review we present the current knowledge on the physiological role of TCSs in species of the families Lactobacillaceae and Leuconostocaceae of the group of lactic acid bacteria (LAB)...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28438014/characterization-of-a-glutamate-decarboxylase-gad-from-enterococcus-avium-m5-isolated-from-jeotgal-a-fermented-korean-sea-food
#13
Kang Wook Lee, Jae Min Shim, Zhuang Yao, Jeong A Kim, Hyun-Jin Kim, Jeong Hwan Kim
To develop starters for the production of functional foods or materials, lactic acid bacteria producing γ-aminobutyric acid (GABA) were screened from jeotgals, Korean fermented sea foods. One isolate producing high amount of GABA from monosodium L-glutamate (MSG) was identified as Enterococcus avium by 16S rRNA gene sequencing. E. avium M5 produced 18.47 ± 1.26 mg/ml GABA when incubated for 48 h at 37°C in MRS broth with MSG (3%, w/v). A gadB gene encoding glutamate decarboxylase (GAD) was cloned and overexpressed in E...
April 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28437625/imaging-guided-synergetic-therapy-of-orthotopic-transplantation-tumor-by-superselectively-arterial-administration-of-microwave-induced-microcapsules
#14
Shunsong Tang, Changhui Fu, Longfei Tan, Tianlong Liu, Jingsong Mao, Xiangling Ren, Hongying Su, Dan Long, Qianqian Chai, Zhongbing Huang, Xudong Chen, Jingzhuo Wang, Jun Ren, Xianwei Meng
It is an ambitious target to improve overall Hepatocellular Carcinoma therapeutic effects. Recently, MW ablation has emerged as a powerful thermal ablation technique, affording favorable survival with excellent local tumor control. To achieve better therapeutic effects of MW ablation, MW sensitizers are prepared for enhanced MW ablation to preferentially heat tumor territory. However, it is still not practicable for treatment of the orthotopic transplantation tumor. Herein, biocompatible and degradable methoxy poly(ethylene glycol)-poly(lactic-co-glycolic acid) (mPEG-PLGA) microcapsules with hierarchical structure have been designed for microwave-induced tumor therapy...
April 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28436251/sirolimus-coating-on-heparinized-stents-prevents-restenosis-and-thrombosis
#15
In-Ho Bae, Kyung Seob Lim, Dae Sung Park, Jae-Won Shim, So-Youn Lee, Eun-Jae Jang, Jun-Kyu Park, Ju-Han Kim, Myung Ho Jeong
The aim of this study was to evaluate the inhibitory effect of sirolimus coating on the occurrence of restenosis and thrombosis with heparinized stents. Heparin and dopamine were conjugated by chemical bonding and anchored on the stent surface by a mussel-inspired adhesion mechanism. Subsequently, sirolimus was coated with poly lactic-glycolic acid on the heparinized stent surface. The heparin was well attached to the surface, and the surface was smooth after sirolimus coating. The smoothness of the surface was maintained after expansion of the stent...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28436165/production-of-high-concentration-of-l-lactic-acid-from-oil-palm-empty-fruit-bunch-by-thermophilic-b-coagulans-ji12
#16
Veeresh Juturu, Jin Chuan Wu
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch (EFB) at 50°C and pH6, producing 105.4 g/L of L-lactic acid with a productivity of 9.3 g/L/h by fed batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH5.5 and 50°C to convert both hemicellulose hydrolysate and cellulose-lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114...
April 24, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28435139/the-role-of-lactic-acid-production-by-probiotic-lactobacillus-species-in-vaginal-health
#17
Gilda Tachedjian, Muriel Aldunate, Catronia S Bradshaw, Richard A Cone
Vaginal eubiosis is characterised by beneficial lactobacillus-dominated microbiota. In contrast, vaginal dysbiosis (e.g. bacterial vaginosis, BV), characterised by an overgrowth of multiple anaerobes, is associated with an increased risk of adverse urogenital and reproductive health outcomes. A major distinguishing feature between the vaginal environment in states of eubiosis and dysbiosis is a high concentration of lactic acid, produced by lactobacilli, that acidifies the vagina in eubiosis versus a sharp drop in lactic acid and an increase in pH in dysbiosis...
April 20, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28434977/starch-fueled-microbial-fuel-cells-by-two-step-and-parallel-fermentation-using-shewanella-oneidensis-mr-1-and-streptococcus-bovis-148
#18
Megumi Uno, Nichanan Phansroy, Yuji Aso, Hitomi Ohara
Shewanella oneidensis MR-1 generates electricity from lactic acid, but cannot utilize starch. On the other hand, Streptococcus bovis 148 metabolizes starch and produces lactic acid. Therefore, two methods were trialed for starch-fueled microbial fuel cell (MFC) in this study. In electric generation by two-step fermentation (EGT) method, starch was first converted to lactic acid by S. bovis 148. The S. bovis 148 were then removed by centrifugation, and the fermented broth was preserved for electricity generation by S...
April 20, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28434892/effective-humoral-immune-response-from-a-h1n1-dna-vaccine-delivered-to-the-skin-by-microneedles-coated-with-plga-based-cationic-nanoparticles
#19
Hae-Yong Seok, Jin Yong No, Dae Yong Lee, Sung Jin Kim, Chang Seon Song, Yeu Chun Kim
Intradermal DNA vaccination is a promising method of immunization that overcomes some practical drawbacks of conventional intramuscular vaccinations. However, it is difficult to deliver DNA vaccines to target cells in the skin and polyplexes. This study outlines the development of an intradermal pH1N1 DNA vaccine delivery platform using microneedles (MNs) coated with a polyplex containing poly lactic-co-glycolic acid/polyethyleneimine (PLGA/PEI) nanoparticles (NPs). Stainless steel MNs with enhanced hydrophilicity have been manufactured by silanization, which improves coating efficiency...
April 20, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28434721/characterization-of-probiotic-bacteria-involved-in-fermented-milk-processing-enriched-with-folic-acid
#20
Zhen Wu, Jing Wu, Pei Cao, Yifeng Jin, Daodong Pan, Xiaoqun Zeng, Yuxing Guo
Yogurt products fermented with probiotic bacteria are a consumer trend and a challenge for functional food development. So far, limited research has focused on the behavior of the various probiotic strains used in milk fermentation. In the present study, we characterized folic acid production and the sensory and textural characteristics of yogurt products fermented with probiotic bacteria. Yogurt fermented with Lactobacillus plantarum had improved nutrient content and sensory and textural characteristics, but the presence of L...
April 20, 2017: Journal of Dairy Science
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