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https://www.readbyqxmd.com/read/28445986/high-bmi1-mrna-expression-in-peripheral-whole-blood-is-associated-with-favorable-prognosis-in-advanced-non-small-cell-lung-cancer-patients
#1
Ana Koren, Matija Rijavec, Eva Sodja, Izidor Kern, Aleksander Sadikov, Viljem Kovac, Peter Korosec, Tanja Cufer
Polycomb group member protein BMI1 is involved in maintaining cell identity, proliferation, differentiation and human oncogenesis. In the present study, we determined BMI1 mRNA expression in whole blood and evaluated the impact of the expression level on the treatment response and survival of 96 advanced NSCLC patients treated with first-line platinum-based chemotherapy. We also determined BMI1 mRNA expression in primary tumors from 22 operable NSCLC patients treated with radical surgery. We found that compared with control subjects, BMI1 mRNA expression in whole blood of advanced NSCLC patients was decreased (P<0...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445901/comparative-pharmacokinetics-of-cholecalciferol-in-dogs-from-2-different-oral-formulations-using-corrective-measures-to-overcome-interference-from-endogenous-cholecalciferol
#2
Harilal Patel, Prakash Patel, Chandrakant Bhatt, Ashok Ghoghari, Urvesh Patel, Mukesh Ukawalal, Shafiq Sheikh, Vikram Ramanathan, Nuggehally R Srinivas
The aim of the preclinical investigation was to obtain single dose pharmacokinetics in dogs from 2 different oral cholecalciferol formulations using corrective measures to overcome the interference of endogenous cholecalciferol. 6 dogs were fasted overnight and the following day received 60 000 IU dose cholecalciferol [reference, Eris(®), vs. test, Sunbless(®)] by oral dosing. Blood samples were collected on day 0 (baseline establishment) and after dosing on day 1 up to 28 days. The serum samples were extracted using protein precipitation/solid phase extraction and analysed to determine cholecalciferol by LC-MS/MS assay with calibrators prepared from cholecalciferol free serum...
April 26, 2017: Drug Research
https://www.readbyqxmd.com/read/28445861/the-application-of-silicon-sol-gel-technology-to-forensic-blood-substitute-development-investigation-of-the-spreading-dynamics-onto-a-paper-surface
#3
Theresa Stotesbury, Mike Illes, Paul Wilson, Andrew J Vreugdenhil
This work investigates the spreading dynamics of three candidate sol-gel solutions, of ranging viscosities, surface tensions and densities, and compares them with water and two commercial blood substitute products. Droplets were created with different sizes (10 to75μL) and impact velocities (1.4 to 6.0m/s) to strike 176gsm cardstock. Over 2200 droplets were created using the six different fluids and their final dried stain diameter was measured. Droplet spread was plotted using the Scheller and Bousfield correlation and uses effective viscosity as a parameter for non-Newtonian fluids...
April 1, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28445848/a-systematic-and-critical-review-on-bioanalytical-method-validation-using-the-example-of-simultaneous-quantitation-of-antidiabetic-agents-in-blood
#4
REVIEW
Mariana Millan Fachi, Letícia Paula Leonart, Letícia Bonancio Cerqueira, Flavia Lada Degaut Pontes, Michel Leandro de Campos, Roberto Pontarolo
A systematic and critical review was conducted on bioanalytical methods validated to quantify combinations of antidiabetic agents in human blood. The aim of this article was to verify how the validation process of bioanalytical methods is performed and the quality of the published records. The validation assays were evaluated according to international guidelines. The main problems in the validation process are pointed out and discussed to help researchers to choose methods that are truly reliable and can be successfully applied for their intended use...
April 13, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28445826/exposure-to-fine-particulate-matter-causes-oxidative-and-methylated-dna-damage-in-young-adults-a-longitudinal-study
#5
Ching-Huang Lai, Han-Bin Huang, Yue-Cune Chang, Ting-Yao Su, Ying-Chuan Wang, Gia-Chi Wang, Jia-En Chen, Chin-Sheng Tang, Trong-Neng Wu, Saou-Hsing Liou
An increased understanding is needed of the physiological effects and plausible biological mechanisms that link PM2.5 (particulate matter with an aerodynamic diameter below 2.5μm) exposure to mortality and morbidities such as atherosclerosis and respiratory disease. PM2.5 causes carcinogenic health effects. Biomonitoring in humans has suggested that 8-oxo-7, 8-dihydro-2-deoxyguanosine (8-oxodG) and N7-methylguanine (N7-MeG) are correlated with oxidative and methylated DNA damage. Thus, it is meaningful to explore the mechanisms of mutagenesis and carcinogenesis associated with oxidative and methylated DNA damage by simultaneously measuring these two markers...
April 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28445506/phenotype-function-and-differentiation-potential-of-human-monocyte-subsets
#6
Lisa B Boyette, Camila Macedo, Kevin Hadi, Beth D Elinoff, John T Walters, Bala Ramaswami, Geetha Chalasani, Juan M Taboas, Fadi G Lakkis, Diana M Metes
Human monocytes have been grouped into classical (CD14++CD16-), non-classical (CD14dimCD16++), and intermediate (CD14++CD16+) subsets. Documentation of normal function and variation in this complement of subtypes, particularly their differentiation potential to dendritic cells (DC) or macrophages, remains incomplete. We therefore phenotyped monocytes from peripheral blood of healthy subjects and performed functional studies on high-speed sorted subsets. Subset frequencies were found to be tightly controlled over time and across individuals...
2017: PloS One
https://www.readbyqxmd.com/read/28445239/aldosterone-and-vascular-mineralocorticoid-receptors-in-murine-endotoxic-and-human-septic-shock
#7
Fouad Fadel, Gwennan André-Grégoire, Basile Gravez, Brigitte Bauvois, Sandrine Bouchet, Catalina Sierra-Ramos, Andrea Polito, Arnaud Mansart, Diego Alvarez de la Rosa, Djillali Annane, Frédéric Jaisser
OBJECTIVES: Vascular mineralocorticoid receptors play a role in vascular tone and blood pressure regulation, might participate in the pathophysiology of circulatory failure during sepsis, and represent a potential therapeutic target in this disease. We aimed to study the effects of mineralocorticoids and the involvement of vascular mineralocorticoid receptors in murine endotoxic and human septic shock. DESIGN: Experimental study. SETTING: Translational investigation including animal research and in vitro experiments using human vascular cells and plasma from septic patients...
April 25, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28445144/stroma-derived-hgf-drives-metabolic-adaptation-of-colorectal-cancer-to-angiogenesis-inhibitors
#8
Alessia Mira, Virginia Morello, Maria Virtudes Céspedes, Timothy Perera, Paolo M Comoglio, Ramon Mangues, Paolo Michieli
The role of paracrine Hepatocyte Growth Factor (HGF) in the resistance to angiogenesis inhibitors (AIs) is hidden in xenograft models because mouse HGF fails to fully activate human MET. To uncover it, we compared the efficacy of AIs in wild-type and human HGF knock-in SCID mice bearing orthotopic human colorectal tumors. Species-specific HGF/MET signaling dramatically impaired the response to anti-angiogenic agents and boosted metastatic dissemination. In cell-based assays mimicking the consequences of anti-angiogenic therapy, colorectal cancer cells were completely resistant to hypoxia but extremely sensitive to nutrient deprivation...
April 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28445076/image-based-phenotypic-screening-with-human-primary-t-cells-using-one-dimensional-imaging-cytometry-with-self-tuning-statistical-gating-algorithms
#9
Steve S Wang, Daniel J Ehrlich
The parallel microfluidic cytometer (PMC) is an imaging flow cytometer that operates on statistical analysis of low-pixel-count, one-dimensional (1D) line scans. It is highly efficient in data collection and operates on suspension cells. In this article, we present a supervised automated pipeline for the PMC that minimizes operator intervention by incorporating multivariate logistic regression for data scoring. We test the self-tuning statistical algorithms in a human primary T-cell activation assay in flow using nuclear factor of activated T cells (NFAT) translocation as a readout and readily achieve an average Z' of 0...
April 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28445008/prevalence-of-complement-mediated-cell-lysis-like-gene-sicg-in-streptococcus-dysgalactiae-subsp-equisimilis-isolates-from-japan-2014-2016
#10
Takashi Takahashi, Tomohiro Fujita, Akiyoshi Shibayama, Yuzo Tsuyuki, Haruno Yoshida
BACKGROUND: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. METHODS: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444973/in-depth-comparative-phenotyping-of-blood-innate-myeloid-leukocytes-from-healthy-humans-and-macaques-using-mass-cytometry
#11
Jamila Elhmouzi-Younes, Jean-Louis Palgen, Nicolas Tchitchek, Simon Delandre, Inana Namet, Caroline L Bodinham, Kathleen Pizzoferro, David J M Lewis, Roger Le Grand, Antonio Cosma, Anne-Sophie Beignon
Comparative immune-profiling of innate responses in humans and non-human primates is important to understand the pathogenesis of infectious and chronic inflammatory diseases as well as for the preclinical development of vaccines and immune therapies. However, direct comparisons of the two species are rare and were never performed using mass cytometry. Here, whole-blood-derived leukocytes from healthy humans and cynomolgus macaques were analyzed with mass cytometry. Two similar panels of around 30 monoclonal antibodies targeting human markers associated with innate myeloid cells to stain fixed human and macaque leukocytes were constructed...
April 26, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28444687/creutzfeldt-jakob-disease-lookback-study-21-years-of-surveillance-for-transfusion-transmission-risk
#12
Lauren A Crowder, Lawrence B Schonberger, Roger Y Dodd, Whitney R Steele
BACKGROUND: Transfusion transmission of human prion diseases has been observed for variant Creutzfeldt-Jakob disease (vCJD), but not for the classic forms of prion disease (CJD: sporadic, genetic, and iatrogenic). Although the presence of prions or misfolded prion proteins in blood has been documented in some patients with the most common form of CJD, sporadic CJD, no transfusion-transmitted cases of CJD have been recognized. Since 1995, the American Red Cross has conducted a lookback study of the recipients of blood products from donors who develop CJD to assess the risk of blood-borne CJD transmission in the United States...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28444619/the-reference-cube-a-new-ballistic-model-to-generate-staining-in-firearm-barrels
#13
Christian Schyma, Kristina Bauer, Julia Brünig
After contact shots to the head biological traces can be found inside firearm barrels. So far silicone coated, gelatin filled box models were used to generate such staining according to the triple contrast method (mixture of acrylic paint, barium sulfate and blood sealed in a thin foil bag). This study was conducted to develop a transparent ballistic model allowing contact shots. Gelatin filled polyethylene bottles with and without a silicone coat were tested in comparison to non-covered gelatin blocks. Finally, thin foil bags of 5 cm × 5 cm dimension were glued on a synthetic absorbent kitchen wipe on top of which 1 L 10% gelatin solution was molded to create blocks of 8...
April 25, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28444186/frataxin-deficiency-impairs-mitochondrial-biogenesis-in-cells-mice-and-humans
#14
Mittal J Jasoliya, Marissa Z McMackin, Chelsea K Henderson, Susan L Perlman, Gino A Cortopassi
Friedreich's ataxia (FRDA) is a neurodegenerative disease caused by inherited deficiency of the mitochondrial protein Frataxin (FXN), which has no approved therapy and is an area in which biomarkers are needed for clinical development. Here we investigated the consequences of FXN deficiency in patient derived FRDA fibroblast cell models, the FRDA mouse model KIKO, and in whole blood collected from FRDA patients. We observed decreased mitochondrial copy number in all the three FRDA models tested: cells, mice and patient blood...
April 21, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28444175/predictive-value-of-telomere-length-on-outcome-following-acute-myocardial-infarction-evidence-for-contrasting-effects-of-vascular-vs-blood-oxidative-stress
#15
Marios Margaritis, Fabio Sanna, George Lazaros, Ioannis Akoumianakis, Sheena Patel, Alexios S Antonopoulos, Chloe Duke, Laura Herdman, Costas Psarros, Evangelos K Oikonomou, Cheerag Shirodaria, Mario Petrou, Rana Sayeed, George Krasopoulos, Regent Lee, Dimitris Tousoulis, Keith M Channon, Charalambos Antoniades
Aims: Experimental evidence suggests that telomere length (TL) is shortened by oxidative DNA damage, reflecting biological aging. We explore the value of blood (BTL) and vascular TL (VTL) as biomarkers of systemic/vascular oxidative stress in humans and test the clinical predictive value of BTL in acute myocardial infarction (AMI). Methods and results: In a prospective cohort of 290 patients surviving recent AMI, BTL measured on admission was a strong predictor of all-cause [hazard ratio (HR) [95% confidence interval (CI)]: 3...
April 24, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28443896/an-msn-peg-ip-drug-delivery-system-and-il13r%C3%AE-2-as-targeted-therapy-for-glioma
#16
Jinlong Shi, Shiqiang Hou, Jianfei Huang, Shanshan Wang, Wei Huan, Chuanjun Huang, Xiaojiang Liu, Rui Jiang, Wenbo Qian, Jingjing Lu, Xiubing Wang, Wei Shi, Rongqin Huang, Jian Chen
A combination of gene therapy and chemotherapy has recently received interest as a targeted therapy for glioma. A mesoporous silica nanoparticle (MSN)-based vehicle coated with IL13Rα2-targeted peptide (IP) using polyethylene glycol (PEG), MSN-PEG-IP (MPI), was constructed and confirmed as a potential glioma-targeted drug delivery system in vitro. In this work, tissue microarray (TMA) results revealed that IL13Rα2 was over-expressed in human glioma tissues and that high expression of IL13Rα2 in patients was associated with poor survival...
April 26, 2017: Nanoscale
https://www.readbyqxmd.com/read/28443724/genetic-diversity-of-hiv-1-gene-vif-among-treatment-na%C3%A3-ve-brazilians
#17
Fabiola Villanova, Marta Barreiros, Luiz Mario Janini, Ricardo Sobhie Diaz, Elcio Leal
HIV-1 has the Vif protein, which binds to human antiviral proteins APOBEC3 to form complexes to be degraded by cellular proteolysis. To further explore HIV-1 diversity at the population level, we analyzed blood samples from 317 treatment-naïve patients in Brazil. Here, we explored the correlations of Vif polymorphisms with clinical parameters of the patients and found that mutation K22H is associated with low CD4+ cell counts and higher viral loads. Phylogenetic analysis of the vif gene indicated that subtype B was predominant in ∼77% (243/317) of the patients, followed by HIV-1 F ∼18% (56/317) and subtype C ∼4% (12/317); five samples were BF recombinants (∼1% of patients), and one was an AG recombinant...
April 26, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28443631/a-peripheral-epigenetic-signature-of-immune-system-genes-is-linked-to-neocortical-thickness-and-memory
#18
Virginie Freytag, Tania Carrillo-Roa, Annette Milnik, Philipp G Sämann, Vanja Vukojevic, David Coynel, Philippe Demougin, Tobias Egli, Leo Gschwind, Frank Jessen, Eva Loos, Wolfgang Maier, Steffi G Riedel-Heller, Martin Scherer, Christian Vogler, Michael Wagner, Elisabeth B Binder, Dominique J-F de Quervain, Andreas Papassotiropoulos
Increasing age is tightly linked to decreased thickness of the human neocortex. The biological mechanisms that mediate this effect are hitherto unknown. The DNA methylome, as part of the epigenome, contributes significantly to age-related phenotypic changes. Here, we identify an epigenetic signature that is associated with cortical thickness (P=3.86 × 10(-8)) and memory performance in 533 healthy young adults. The epigenetic effect on cortical thickness was replicated in a sample comprising 596 participants with major depressive disorder and healthy controls...
April 26, 2017: Nature Communications
https://www.readbyqxmd.com/read/28443622/the-rudy-study-using-digital-technologies-to-enable-a-research-partnership
#19
Harriet J A Teare, Joanna Hogg, Jane Kaye, Raashid Luqmani, Elaine Rush, Alison Turner, Laura Watts, Melanie Williams, M Kassim Javaid
Patients have extensive experience of their disease that can enhance the design and execution of research leading to significant innovations and efficiencies in the research process. The research community on the whole have been slow to adopt practices that enable patients to become active partners in research. Digital technologies are providing the means to do this more easily and so are increasingly being used to interact with patients and involve them in the design and execution of research. The RUDY (Rare UK Diseases of bone, joints and blood vessels) study's pioneering approach applies a custom-developed electronic platform where patients can contribute information over time about their disease experience, lifestyle and clinical history...
April 26, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/28443506/catestain-a-master-regulator-of-cardiovascular-functions
#20
Sushil K Mahata, Malapaka Kiranmayi, Nitish R Mahapatra
Cardiovascular disease (CVD), the most common cause of death globally, accounts for ~30% of all deaths worldwide. Hypertension is a common contributor to morbidity and mortality from CVD. The plasma concentration of chromogranin A (CgA) is elevated in patients with CVD as well as patients with established human essential hypertension and heart failure (HF). In contrast, the plasma level of the CgA-derived peptide catestatin (CST) is diminished in human essential hypertension. Low conversion of CgA-to-CST has been associated with increased mortality in patients hospitalized with acute HF...
April 24, 2017: Current Medicinal Chemistry
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