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https://www.readbyqxmd.com/read/28109772/trichoderma-virens-as-a-biocontrol-of-toxocara-canis-in-vivo-evaluation
#1
Fernando de Souza Maia Filho, Anelise Oliveira da Silva Fonseca, Beatriz Maroneze Persici, Julia de Souza Silveira, Caroline Quintana Braga, Luciana Pötter, Sônia de Avila Botton, Daniela Isabel Brayer Pereira
BACKGROUND: Microorganisms have been widely studied as biological control agents of parasites of medical and veterinary importance. Coprophagous arthropods, bacteria and fungi are among the different organisms evaluated as potential biological control agents. Nematophagous fungi capture and digest the free forms of nematodes in the soil. Due to its zoonotic potential, Toxocara canis have been brought to the attention of researchers. AIMS: The aim of the present study was to determine whether the administration of embryonated T...
January 18, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28108330/pleiotropic-responses-to-methionine-restriction
#2
Gene Ables, Jay Johnson
Methionine restriction (MR) extends lifespan across different species. The main responses of rodent models to MR are well-documented in adipose tissue (AT) and liver, which have reduced mass and improved insulin sensitivity, respectively. Recently, molecular mechanisms that improve healthspan have been identified in both organs during MR. In fat, MR induced a futile lipid cycle concomitant with beige AT accumulation, producing elevated energy expenditure. In liver, MR upregulated fibroblast growth factor 21 and improved glucose metabolism in aged mice and in response to a high-fat diet...
January 17, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28105919/snoreport-2-0-new-features-and-a-refined-support-vector-machine-to-improve-snorna-identification
#3
João Victor de Araujo Oliveira, Fabrizio Costa, Rolf Backofen, Peter Florian Stadler, Maria Emília Machado Telles Walter, Jana Hertel
BACKGROUND: snoReport uses RNA secondary structure prediction combined with machine learning as the basis to identify the two main classes of small nucleolar RNAs, the box H/ACA snoRNAs and the box C/D snoRNAs. Here, we present snoReport 2.0, which substantially improves and extends in the original method by: extracting new features for both box C/D and H/ACA box snoRNAs; developing a more sophisticated technique in the SVM training phase with recent data from vertebrate organisms and a careful choice of the SVM parameters C and γ; and using updated versions of tools and databases used for the construction of the original version of snoReport...
December 15, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/28103957/rna-interference-mediated-knockdown-of-brugia-malayi-udp-galactopyranose-mutase-severely-affects-parasite-viability-embryogenesis-and-in-vivo-development-of-infective-larvae
#4
Sweta Misra, Jyoti Gupta, Shailja Misra-Bhattacharya
BACKGROUND: Galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as Caenorhabditis spp., Brugia spp., Onchocerca spp. and Strongyloides spp. This sugar maintains the integrity of parasite surface and is essential for virulence. UDP-Galactopyranose mutase (bmugm) plays a key role in Galf biosynthesis by catalyzing conversion of UDP-Galactopyranose into UDP-galactofuranose and knockout studies of the gene in Leishmania major, Mycobacterium and Aspergillus fumigatus displayed attenuated virulence while RNA interference study in C...
January 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28103804/inactivity-periods-and-postural-change-speed-can-explain-atypical-postural-change-patterns-of-caenorhabditis-elegans-mutants
#5
Tsukasa Fukunaga, Wataru Iwasaki
BACKGROUND: With rapid advances in genome sequencing and editing technologies, systematic and quantitative analysis of animal behavior is expected to be another key to facilitating data-driven behavioral genetics. The nematode Caenorhabditis elegans is a model organism in this field. Several video-tracking systems are available for automatically recording behavioral data for the nematode, but computational methods for analyzing these data are still under development. RESULTS: In this study, we applied the Gaussian mixture model-based binning method to time-series postural data for 322 C...
January 19, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28103637/exploiting-cell-cycle-inhibitor-genes-of-the-krp-family-to-control-root-knot-nematode-induced-feeding-sites-in-plants
#6
Roberta Ramos Coelho, Paulo Vieira, José Dijair Antonino de Souza Júnior, Cristina Martin-Jimenez, Lieven De Veylder, Julie Cazareth, Gilbert Engler, Maria Fatima Grossi-de-Sa, Janice de Almeida Engler
Cell cycle control in galls provoked by root-knot nematodes involves the activity of inhibitor genes like the Arabidopsis ICK/KRP members. Ectopic KRP1, KRP2 and KRP4 expression resulted in decreases gall size by inhibiting mitotic activity, whereas KRP6 induces mitosis in galls. Herein, we investigate the role of KRP3, KRP5 and KRP7 during gall development and compared their role with previously studied members of this class of cell cycle inhibitors. Overexpression of KRP3 and KRP7 resulted in undersized giant cells, with KRP3(OE) galls presenting peculiar elongated giant cells...
January 19, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28103386/paraoxonase-1-activity-is-related-to-trichinella-spiralis-induced-hepatitis-in-rats
#7
Ayman Samir Farid, Eman Mohamed Fath, Shogo Mido, Nariaki Nonaka, Yoichiro Horii
BACKGROUND: Little is known about the potential adverse effects of a chronic zoonotic nematode Trichinella spiralis infection on hepatic inflammation and its relationship to paraoxonase (PON)-1 and butyrylcholinesterase (BuChE) activities. Therefore, we aimed to examine the effects of T. spiralis infection on hepatic synthesis of PON1. METHODS: Wistar rats were infected with 2500 first-stage larvae of T. spiralis and serum PON1 and BuChE activities were evaluated...
January 19, 2017: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28101647/a-real-time-pcr-approach-to-identify-anthelmintic-resistant-nematodes-in-sheep-farms
#8
M Milhes, M Guillerm, M Robin, M Eichstadt, C Roy, C Grisez, F Prévot, E Liénard, E Bouhsira, M Franc, P Jacquiet
Resistance to fenbendazole, ivermectin, and moxidectin was explored by a fecal egg count reduction test in four meat sheep flocks in southwestern France where anthelmintic resistance was suspected. The FECR test results of the present study confirmed the presence of benzimidazole resistance in three out of the four farms and the presence of ivermectin resistance in one flock. In addition, a suspicion of moxidectin resistance was shown in this latter farm. Both conventional morphological and molecular identifications were performed on larval cultures before and after the treatment in the studied farms...
January 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28099087/effects-of-biochar-on-the-emissions-soil-distribution-and-nematode-control-of-1-3-dichloropropene
#9
Daniel J Ashworth, Scott R Yates, Guoqing Shen
Emissions of volatile soil fumigant 1,3-dichloropropene (1,3-D) from soil to air are a significant concern in relation to air quality, and cost-effective strategies to reduce such emissions are urgently required by growers to help them comply with increasingly stringent regulations. In this work, application of a rice husk-derived biochar to the surface of a sandy loam soil chamber reduced soil-air emissions of 1,3-D from 42% in a control (no biochar) to 8% due to adsorption onto the biochar. This adsorbed 1,3-D showed a potential for re-volatilization into air and solubilization into the soil-liquid phase...
February 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28098555/nematophagous-fungus-arthrobotrys-oligospora-mimics-olfactory-cues-of-sex-and-food-to-lure-its-nematode-prey
#10
Yen-Ping Hsueh, Matthew R Gronquist, Erich M Schwarz, Ravi David Nath, Ching-Han Lee, Shalha Gharib, Frank C Schroeder, Paul W Sternberg
To study the molecular basis for predator-prey coevolution, we investigated how Caenorhabditis elegans responds to the predatory fungus Arthrobotrys oligospora. C. elegans and other nematodes were attracted to volatile compounds produced by A. oligospora. Gas-chromatographic mass-spectral analyses of A. oligospora-derived volatile metabolites identified several odors mimicking food cues attractive to nematodes. One compound, methyl 3-methyl-2-butenoate (MMB) additionally triggered strong sex- and stage-specific attraction in several Caenorhabditis species...
January 18, 2017: ELife
https://www.readbyqxmd.com/read/28098549/diffuse-unilateral-subacute-neuroretinitis-caused-by-ancylostoma-hookworm
#11
Sven Poppert, Martin Heideking, Hansjürgen Agostini, Moritz Fritzenwanker, Nicole Wüppenhorst, Birgit Muntau, Philipp Henneke, Winfried Kern, Jürgen Krücken, Bernd Junker, Markus Hufnagel
Diffuse unilateral subacute neuroretinitis is an ocular infectious disease caused by several distinct nematodes. Definite identification of the involved nematodes is rarely achieved. We report on the molecular-based genetic identification of an Ancylostoma ceylanicum hookworm implicated in a case of diffuse unilateral subacute neuroretinitis in a child.
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28098507/development-and-evaluation-of-a-loop-mediated-isothermal-amplification-lamp-assay-for-the-detection-of-haemonchus-contortus-in-goat-fecal-samples
#12
Xin Yang, Mingwei Qi, Zongze Zhang, Chong Gao, Chunqun Wang, Weiqiang Lei, Li Tan, Junlong Zhao, Rui Fang, Min Hu
Haemonchus contortus is one of the most significant strongylid nematodes infecting small ruminants and causes great economic losses to the livestock industry worldwide. Accurate diagnosis of H. contortus is crucial to control the infection. Traditional microscopic examinations are the most common methods for the diagnosis of H. contortus, but they are time-consuming and inaccurate. Molecular methods based on PCR are more accurate, but need expensive machines usually only used in the laboratory. Loop-mediated isothermal amplification (LAMP) is a rapid, simple, specific and sensitive method that has been widely used to detect viruses, bacteria and parasites...
January 18, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28096864/feline-dirofilariosis-due-to-dirofilaria-immitis-in-meshkin-shahr-district-northwestern-iran
#13
Mahya Khodabakhsh, Abdolali Malmasi, Mehdi Mohebali, Zabihollah Zarei, Eshrat Beigom Kia, Amrollah Azarm
BACKGROUND: Dirofilaria immitis is a common nematode of the cardiovascular system, which infects carnivores all over the world. The infection is prevalent in dogs, but in cats and human is rare. Dirofilariosis is transmitted by mosquitoes. Cats are accidental hosts and are naturally resistant to the infection, compared to the dogs. Mild infection can cause severe illness in cats and may lead to death, so it is clinically important to diagnose the disease. METHODS: In the present study, 103 stray cats were collected from Meshkin Shahr district, Aradabil Province, northwestern Iran that is an endemic area for canine dirofilariosis...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096863/seroepidemiological-study-of-toxocariasis-in-the-owners-of-domestic-cats-and-dogs-in-mashhad-northeastern-iran
#14
Fariba Berenji, Ali Pouryousef, Abdolmajid Fata, Mahmoud Mahmoudi, Maryam Salehi, Javad Khoshnegah
BACKGROUND: Toxocariasis is the clinical terms applied to infection of human with Ascarid nematodes in the order Ascaridida, named Toxocara canis and T. cati. Because in recent years in Iran many people desire to keep pets (cats and dogs), and lacking of seroepidemiological study of toxocariasis in Mashhad, we decided to determine the seroprevalence of toxocariasis among people who own cats and dogs in comparison with control group. METHODS: A serological study for detection antibodies to Toxocara in two groups (93 cat and dog owners and 93 healthy people as control group) was conducted from Feb 2013 to Dec 2013...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096859/occurrence-and-intensity-of-anisakid-nematode-larvae-in-some-commercially-important-fish-species-in-persian-gulf
#15
Maryam Dadar, Alireza Alborzi, Rahim Peyghan, Milad Adel
BACKGROUND: Anisakid nematodes are common parasites of fish, mammals, fish-eating birds, and reptiles with a worldwide distribution, causing diseases in human, fish and important economic losses. METHODS: A preliminary epidemiological study was carried out on Anisakid nematodes larvae in some commercially important fish species to evaluate the anisakid nematode larvae from greater lizardfish, (Saurida tumbil), Japanese thread fin bream (Nemipterus japonicus), crocodile longtom (Tylosurus crocodilus crocodiles) and longfin trevally (Carangoides armatus) from the Persian Gulf of Iran...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28096612/effect-of-feeding-acacia-nilotica-pod-meal-on-hematobiochemical-profile-and-fecal-egg-count-in-goats
#16
Jitendra Kumar Paswan, Kaushalendra Kumar, Sanjay Kumar, Chandramoni, Abhishek Kumar, Deepak Kumar, Ajit Kumar
AIM: This study was conducted to observe the effect of feeding Acacia nilotica pod meal on hematobiochemical profile and gastrointestinal parasitic load in growing goats. MATERIALS AND METHODS: To experiment was conducted for a period of 3-month on 24 male goats (3½ month old, average body weight [BW] 6.50±1.50 kg), distributed into four groups of six animals each. The experimental animals were fed graded level of A. nilotica pod meal (0%, 10%, 20% and 30%) mixed in concentrate mixture equivalent to tannin concentration of 0%, 1...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096603/gastrointestinal-nematode-larvae-in-the-grazing-land-of-cattle-in-guwahati-assam
#17
Meena Das, D K Deka, S Islam, P C Sarmah, K Bhattacharjee
AIM: To know the prevalence of gastrointestinal nematode larvae (L3) in the grazing land of cattle in Guwahati, Kamrup district, Assam. MATERIALS AND METHODS: Pastures were collected and examined for the presence of nematode larvae (L3) from six localities of Guwahati at monthly interval from August 2012 to July 2013. The counted larvae were then expressed as per kg dry matter of herbage (L3/kg DM). RESULTS: Examination of pastures revealed presence of nematode larvae (L3) in pastures throughout the year which varied from 4...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28096462/unique-contributions-of-an-arginine-side-chain-to-ligand-recognition-in-a-glutamate-gated-chloride-channel
#18
Timothy Lynagh, Vitaly V Komnatnyy, Stephan A Pless
Glutamate recognition by neurotransmitter receptors often relies on arginine (Arg) residues in the binding site, leading to the assumption that charge-charge interactions underlie ligand recognition. However, assessing the precise chemical contribution of Arg side chains to protein function and pharmacology has proven to be exceedingly difficult in such large and complex proteins. Using the in vivo nonsense suppression approach, we report the first successful incorporation of the isosteric, titratable Arg analog, canavanine, into a neurotransmitter receptor in a living cell, utilizing a glutamate-gated chloride channel from the nematode Haemonchus contortus...
January 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28096268/the-tgf-%C3%AE-family-in-caenorhabditis-elegans
#19
Cathy Savage-Dunn, Richard W Padgett
Transforming growth factor β (TGF-β) and related ligands have potent effects on an enormous diversity of biological functions in all animals examined. Because of the strong conservation of TGF-β family ligand functions and signaling mechanisms, studies from multiple animal systems have yielded complementary and synergistic insights. In the nematode Caenorhabditis elegans, early studies were instrumental in the elucidation of TGF-β family signaling mechanisms. Current studies in C. elegans continue to identify new functions for the TGF-β family in this organism as well as new conserved mechanisms of regulation...
January 17, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28095927/small-alteration-big-impacts-effects-of-small-scale-riparian-forest-management-on-host-parasite-dynamics-in-streams
#20
C Lagrue, A A Besson, A Lecerf
Environmental changes and ecological disturbances can have large and unpredictable effects on parasite dynamics. Increasing human impacts on freshwater ecosystems through land use may thus modify the distribution and abundance of parasites and have cascading effects on host populations. Here we tested the effects of small-scale riparian forest management on the nematode Cystidicoloides ephemeridarum and its insect intermediate host Ephemera danica in forested streams. We assessed the impacts of harvesting riparian trees on parasite prevalence and abundance concomitantly with host densities...
January 18, 2017: Journal of Helminthology
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