keyword
MENU ▼
Read by QxMD icon Read
search

Scaffold

keyword
https://www.readbyqxmd.com/read/28922823/de-novo-genome-assembly-of-camptotheca-acuminata-a-natural-source-of-the-anti-cancer-compound-camptothecin
#1
Dongyan Zhao, John P Hamilton, Gina M Pham, Emily Crisovan, Krystle Wiegert-Rininger, Brieanne Vaillancourt, Dean DellaPenna, C Robin Buell
Camptotheca acuminata is 1 of a limited number of species that produce camptothecin, a pentacyclic quinoline alkaloid with anti-cancer activity due to its ability to inhibit DNA topoisomerase. While transcriptome studies have been performed previously with various camptothecin-producing species, no genome sequence for a camptothecin-producing species is available to date. We generated a high-quality de novo genome assembly for C. acuminata representing 403 174 860 bp on 1394 scaffolds with an N50 scaffold size of 1752 kbp...
September 1, 2017: GigaScience
https://www.readbyqxmd.com/read/28922752/current-understanding-of-the-pathways-of-flavonoid-biosynthesis-in-model-and-crop-plants
#2
Takayuki Tohge, Leonardo Perez de Souza, Alisdair R Fernie
Flavonoids are a signature class of secondary metabolites formed from a relatively simple collection of scaffolds. They are extensively decorated by chemical reactions including glycosylation, methylation, and acylation. They are present in a wide variety of fruits and vegetables and as such in Western populations it is estimated that 20-50 mg of flavonoids are consumed daily per person. In planta they have demonstrated to contribute to both flower color and UV protection. Their consumption has been suggested to presenta wide range of health benefits...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922629/new-indole-based-chalconoids-as-tubulin-targeting-antiproliferative-agents
#3
Hassan Mirzaei, Mohammad Shokrzadeh, Mona Modanloo, Ali Ziar, Gholam Hossein Riazi, Saeed Emami
Tubulin-targeting compounds have a broad anticancer spectrum and are an important class of chemotherapeutic agents. Due to the importance of 3-bromo-3,5-dimethoxyphenyl scaffold in the anticancer activity of microtubule inhibitors such as crolibulin (EPC2407), we introduced this functionality into the indole-derived chalcones. Thus, we describe here the synthesis and biological evaluation of new indole-based chalconoids as tubulin-targeting antiproliferative agents. The best result was obtained by compound 9b against A549 cell with IC50 of 4...
September 7, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28922596/privileged-substructures-to-modulate-protein-protein-interactions
#4
Nicolas Bosc, Mélaine A Kuenemann, Jérome Bécot, Marek Vavrusa, Adrien H Cerdan, Olivier Sperandio
Given the difficulties to identify chemical probes that can modulate protein-protein interactions (PPIs), actors in the field start to agree on the necessity to use PPI-tailored screening chemical collections. However, which type of scaffolds may promote the binding of compounds to PPI targets remains unclear. In this big data analysis, we have identified a list of privileged chemical substructures that are most often observed within inhibitors of PPIs. Using molecular frameworks as a way to perceive chemical substructures with the combination of an experimental and a machine-learning based predicted dataset of iPPI compounds, we propose a list of privileged substructures in the form of scaffolds and chemical moieties that can be substantially chemically functionalized and do not present any toxicophore nor Pan-assay interference (PAINS) alerts...
September 18, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28922577/functional-n-formyl-peptide-receptor-2-fpr2-antagonists-based-on-ureidopropanamide-scaffold-have-potential-to-protect-against-inflammation-associated-oxidative-stress
#5
Madia Letizia Stama, Enza Lacivita, Liliya N Kirpotina, Mauro Niso, Roberto Perrone, Igor A Schepetkin, Mark T Quinn, Marcello Leopoldo
Formyl peptide receptor-2 (FPR2) is a G protein-coupled receptor belonging to the N-formyl peptide receptor (FPR) family that plays critical roles in peripheral and brain inflammatory responses. FPR2 has been proposed as a target for the development of drugs that could facilitate the resolution of chronic inflammatory reactions by enhancing endogenous anti-inflammation systems. Starting from the structure of the FPR2 agonists (R)- and (S)-4 and 2, we designed a new series of ureidopropanamide derivatives with the goal of converting functional activity from agonism to antagonism and to develop new FPR2 antagonists...
September 18, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28922555/laser-micro-ablation-of-fibrocartilage-tissue-effects-of-tissue-processing-on-porosity-modification-and-mechanics
#6
A M Matuska, P S McFetridge
The temporomandibular joint disk (TMJd) is an extremely dense and avascular fibrocartilaginous extracellular matrix (ECM) resulting in a limited regenerative capacity. The use of decellularized TMJd as a biocompatible scaffold to guide tissue regeneration is restricted by innate subcellular porosity of the ECM that hinders cellular infiltration and regenerative events. Incorporation of an artificial microporosity through laser micro-ablation (LMA) can alleviate these cell and diffusion based limitations. In this study, LMA was performed either before or after decellularization to assess to effect of surfactant treatment on porosity modification as well as the resultant mechanical and physical scaffold properties...
September 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28922543/in-vitro-and-in-vivo-degradation-of-microfiber-bioresorbable-coronary-scaffold
#7
Chi-Hung Huang, Sheng-Yang Lee, Sonida Horng, Louis-Georges Guy, Ting-Bin Yu
The degradation of Mirage Bioresorbable Microfiber Scaffold was evaluated in vitro and in vivo. The degradation in polymer molecular weight (MW), strut morphology, and integrity was accessed using gel permeation chromatography (GPC), X-ray micro-computed tomography (micro-CT) evaluation. To simulate the physiological degradation in vitro, scaffolds were deployed in silicone mock vessels connected to a peristaltic pumping system, which pumps 37°C phosphate-buffered saline (PBS, pH 7.4) at a constant rate. At various time points (30D, 60D, 90D, 180D, 270D, and 360D), the MW of microfibers decreased to 57...
September 18, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28922542/a-diphenyldiselenide-derivative-induces-autophagy-via-jnk-in-htb-54-lung-cancer-cells
#8
Marta Díaz, Roncesvalles González, Daniel Plano, Juan Antonio Palop, Carmen Sanmartín, Ignacio Encío
Symmetric aromatic diselenides are potential anticancer agents with strong cytotoxic activity. In this study, the in vitro anticancer activities of a novel series of diarylseleno derivatives from the diphenyldiselenide (DPDS) scaffold were evaluated. Most of the compounds exhibited high efficacy for inducing cytotoxicity against different human cancer cell lines. DPDS 2, the compound with the lowest mean GI50 value, induced both caspase-dependent apoptosis and arrest at the G0 /G1 phase in acute lymphoblastic leucemia CCRF-CEM cells...
September 18, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28922323/face-graft-scaffold-production-in-a-rat-model
#9
Jérôme Duisit, Hadrien Amiel, Giuseppe Orlando, Adeline Dedriche, Catherine Behets, Pierre Gianello, Benoît Lengelé
BACKGROUND: As a route towards face bioengineering, our group previously reported the production of a complete scaffold production by perfusion-decellularization of a porcine ear subunit graft and partial recellularization. In order to extend the scaffold to the whole face and to down-scale it, we applied our findings to a rodent hemi-facial graft model. METHODS: After euthanasia, seven full-thickness rat hemi-face grafts were harvested with the CCA and the EJV as pedicle, and cannulated...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28921990/disruption-of-asymmetric-lipid-bilayer-models-mimicking-the-outer-membrane-of-gram-negative-bacteria-by-an-active-plasticin
#10
Jean-Philippe Michel, Yingxiong Wang, Irena Kiesel, Yuri Gerelli, Veronique Rosilio
The outer membrane (OM) of Gram-negative bacteria is a complex and asymmetric bilayer that antimicrobial peptides must disrupt in order to provoke the cell lysis. The inner and external leaflets of the OM are mainly composed of phospholipids (PL), and lipopolysaccharide (LPS), respectively. Supported lipid bilayers are interesting model systems to mimic the lipid asymmetric scaffold of the OM and determine the quantitative and mechanistic effect of antimicrobial agents, using complementary physicochemical techniques...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28921822/construction-of-human-induced-pluripotent-stem-cell-derived-oriented-bone-matrix-microstructure-by-using-in-vitro-engineered-anisotropic-culture-model
#11
Ryosuke Ozasa, Aira Matsugaki, Yoshihiro Isobe, Taro Saku, Hui-Suk Yun, Takayoshi Nakano
Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold...
September 16, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28921540/imaging-stem-cell-distribution-growth-migration-and-differentiation-in-3-d-scaffolds-for-bone-tissue-engineering-using-mesoscopic-fluorescence-tomography
#12
Qinggong Tang, Charlotte Piard, Jonathan Lin, Kai Nan, Ting Guo, John Caccamese, John Fisher, Yu Chen
Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2∼3 mm)...
September 16, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28921421/tissue-engineering-of-3d-organotypic-microtissues-by-acoustic-assembly
#13
Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, Sinan Güven
There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.
September 19, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28921079/identification-of-a-new-isoindole-2-yl-scaffold-as-a-qo-and-qi-dual-inhibitor-of-cytochrome-bc-1-complex-virtual-screening-synthesis-and-biochemical-assay
#14
Homa Azizian, Kowsar Bagherzadeh, Sophia Shahbazi, Niusha Sharifi, Massoud Amanlou
Respiratory chain ubiquinol-cytochrome (cyt) c oxidoreductase (cyt bc 1 or complex III) has been demonstrated as a promising target for numerous antibiotics and fungicide applications. In this study, a virtual screening of NCI diversity database was carried out in order to find novel Qo/Qi cyt bc 1 complex inhibitors. Structure-based virtual screening and molecular docking methodology were employed to further screen compounds with inhibition activity against cyt bc 1 complex after extensive reliability validation protocol with cross-docking method and identification of the best score functions...
September 18, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/28920917/study-on-the-regulation-of-focal-adesions-and-cortical-actin-by-matrix-nanotopography-in-3d-environment
#15
Jingjing Han, Keng-Hui Lin, Lock Yue Chew
Matrix nanotopography plays an important role in regulating cell behaviors by providing spatial as well as mechanical cues for cells to sense. It has been proposed that nanoscale topography is possible to modulate the tensions which direct the formation of cytoskeleton and the organization of membrane receptor within the cell, which in turn regulate the intracellular mechanical and biochemical signaling. With current studies on this topic being performed mainly in 2D platforms, the question on how nanotopography can influence cell bahaviors in 3D environments has yet to be addressed...
September 18, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28920846/detection-of-the-incx3-plasmid-carrying-blakpc-3-in-a-serratia-marcescens-strain-isolated-from-a-kidney-liver-transplanted-patient
#16
Floriana Gona, Carla Caio, Gioacchino Iannolo, Francesco Monaco, Giuseppina Di Mento, Nicola Cuscino, Ignazio Fontana, Giovanna Panarello, Gaetano Maugeri, Maria Lina Mezzatesta, Stefania Stefani, Pier Giulio Conaldi
Dissemination of resistance to carbapenems among Enterobacteriaceae through plasmids is an increasingly important concern in health care worldwide. Here we report the first description of an IncX3 plasmid carrying the blaKPC-3 gene in a strain of Serratia marcescens isolated from a kidney-liver transplanted patient at the transplantation centre ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione, Palermo, Italy). To localize the transposable element containing the resistance-associated gene Next-Generation Sequencing of the bacterial DNA was performed...
September 18, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28920688/enantioselective-construction-of-cyclopenta-b-indole-scaffolds-via-the-catalytic-asymmetric-3-2-cycloaddition-of-2-indolylmethanols-with-p-hydroxystyrenes
#17
Meng-Meng Xu, Hai-Qing Wang, Ying Wan, Shu-Liang Wang, Feng Shi
The catalytic asymmetric [3+2] cycloaddition of 2-indolylmethanols to p-hydroxystyrenes was established in the presence of a chiral phosphoramide, and this reaction provided chiral cyclopenta[b]indole scaffolds in generally high yields and with good enantioselectivities (up to 98% yield, 99:1 er). The control experiments demonstrated that the dual hydrogen-bonding activation mode of the chiral catalyst toward the two substrates played an important role in the reaction. In addition, the large-scale reaction indicated that this catalytic asymmetric [3+2] cycloaddition could be scaled up for the synthesis of chiral cyclopenta[b]indole derivatives...
September 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28920399/-atdc-5-growth-promoted-by-sustained-releasing-chitosan-microspheres-loading-tgf-%C3%AE-1-in-artificial-cartilage-scaffolds
#18
Ya'nan Chang, Hao Liu, Chengbao Feng, Xiaoliang He, Xiaohui Zhou
In order to promote the growth of chondrocyte ATDC-5 in collagen type II-hyaluronic acid-chondroitin sulfate composite scaffolds constructed previously in vitro, the sustained-releasing chitosan microspheres loading TGF-β1 were prepared by emulsification and cross-linking. In addition, ATDC-5 was inoculated into the scaffolds incorporating the chitosan microspheres with TGF-β1. Results show that the morphology of microsphere was round and uniform, mean diameter was about 100 nm, absorption rate was up to 983...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28920389/-research-progress-in-multi-enzyme-regulation-of-genetically-engineered-bacteria-producing-lycopene
#19
Jiali Xu, Siyi Zuo, Chengjia Xie, Ling Jiang, Shuang Li, He Huang, Xian Xu
Lycopene plays a crucial role in the biosynthesis pathway of 2-methyl-derythritol-4-phosphate (MEP) and mevalonic acid (MVA). It is a representative product of isoprenoid family, and a typical product of multi-enzyme catalytic reaction in organism. In this paper, we first introduced the general regulation methods in multi-enzyme synthesis reaction, including the construction of multi gene co-expression plasmid, gene order regulation, promoter and ribosome binding site regulation, gene knockout and replacement, aiming at the optimization strategies of multi-enzyme catalytic reaction in lycopene synthesis pathway...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28920113/double-1-4-addition-of-thio-salicylamides-thiosalicylic-acids-with-propiolate-derivatives-a-direct-general-synthesis-of-diverse-heterocyclic-scaffolds
#20
Hui-Hong Wang, Tao Shi, Wei-Wei Gao, Hong-Hua Zhang, Yong-Qiang Wang, Jun-Fang Li, Yong-Sheng Hou, Jin-Hong Chen, Xue Peng, Zhen Wang
A simple and practical ring-closure procedure to prepare a range of diverse heterocycles has been developed. In this transformation, a variety of substituted (thio)salicylamides and thiosalicylic acids undergo a double 1,4-addition reaction with propiolate derivatives in the presence of an inorganic base (K3PO4), as a result benzothiazinones, benzoxazinones and benzoxathiinones were prepared in good to excellent yields, respectively, even in gram scales. In addition, further transformation towards more complex structures and oxicam drug analogues has also been successfully carried out...
September 18, 2017: Organic & Biomolecular Chemistry
keyword
keyword
13178
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"