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https://www.readbyqxmd.com/read/28817771/ice-templated-and-cross-linked-amyloid-fibril-aerogel-scaffolds-for-cell-growth
#1
Gustav Nyström, Wye-Khay Fong, Raffaele Mezzenga
Amyloid fibrils prepared from β-lactoglobulin were used to form freeze-dried and cross-linked aerogels. By varying the fibril concentration and freezing gradient, it was possible to control the pore structure and elastic modulus of the aerogels over one order of magnitude from ~2 to ~200 kPa. Using butane tetracarboxylic acid as cross-linker these aerogels maintained their monolithic shape in aqueous conditions displaying elastic behavior and a modulus in the range of 4-40 kPa. When explored as scaffolds for cell growth, the amyloid fibril aerogels demonstrated biocompatibility and led to the successful penetration and permeation of two epithelial cell lines (Caco-2 and HT29) throughout the scaffold...
August 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28817694/a-new-holistic-3d-non-invasive-analysis-of-cellular-distribution-and-motility-on-fibroin-alginate-microcarriers-using-light-sheet-fluorescent-microscopy
#2
Serena Duchi, Filippo Piccinini, Michela Pierini, Alessandro Bevilacqua, Maria Luisa Torre, Enrico Lucarelli, Spartaco Santi
Cell interaction with biomaterials is one of the keystones to developing medical devices for tissue engineering applications. Biomaterials are the scaffolds that give three-dimensional support to the cells, and are vectors that deliver the cells to the injured tissue requiring repair. Features of biomaterials can influence the behaviour of the cells and consequently the efficacy of the tissue-engineered product. The adhesion, distribution and motility of the seeded cells onto the scaffold represent key aspects, and must be evaluated in vitro during the product development, especially when the efficacy of a specific tissue-engineered product depends on viable and functional cell loading...
2017: PloS One
https://www.readbyqxmd.com/read/28817632/interdependency-and-phosphorylation-of-kif4-and-condensin-i-are-essential-for-organization-of-chromosome-scaffold
#3
Rawin Poonperm, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui
Kinesin family member 4 (KIF4) and condensins I and II are essential chromosomal proteins for chromosome organization by locating primarily to the chromosome scaffold. However, the mechanism of how KIF4 and condensins localize to the chromosome scaffold is poorly understood. Here, we demonstrate a close relationship between the chromosome localization of KIF4 and condensin I, but not condensin II, and show that KIF4 and condensin I assist each other for stable scaffold formation by forming a stable complex...
2017: PloS One
https://www.readbyqxmd.com/read/28817473/scaffolding-interprofessional-education-unfolding-case-studies-virtual-world-simulations-and-patient-centered-care
#4
Sharon Strang Zook, Linda J Hulton, Carol C Dudding, Anne L Stewart, Amy C Graham
Fragmentation of health care negatively impacts quality; one of the contributing factors may be ineffective collaboration among health care professionals. This article describes the implementation of an interprofessional education curriculum for graduate students enrolled in nursing, psychology, and speech-language pathology programs. Over 3 semesters, students engaged in interprofessional collaboration modules, unfolding case studies, virtual simulation, and shared case planning experiences. The curriculum's impact on students' attitudes and values toward interprofessional collaborative practice was measured...
August 16, 2017: Nurse Educator
https://www.readbyqxmd.com/read/28817384/engineering-the-mechanical-and-biological-properties-of-nanofibrous-vascular-grafts-for-in-situ-vascular-tissue-engineering
#5
Jeffrey J D Henry, Jian Yu, Aijun Wang, Randall Lee, Jun Fang, Song Li
Synthetic small diameter vascular grafts have a high failure rate, and endothelialization is critical for preventing thrombosis and graft occlusion. A promising approach is in situ tissue engineering, whereby an acellular scaffold is implanted and provides stimulatory cues to guide the in situ remodeling into a functional blood vessel. An ideal scaffold should have sufficient binding sites for biomolecule immobilization and a mechanical property similar to native tissue. Here we developed a novel method to blend low molecular weight (LMW) elastic polymer during electrospinning process to increase conjugation sites and to improve the mechanical property of vascular grafts...
August 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/28817145/coherent-electronic-and-nuclear-dynamics-in-a-rhodamine-heterodimer-dna-supramolecular-complex
#6
M Cipolloni, B Fresch, I Occhiuto, P Rukin, K G Komarova, A Cecconello, I Willner, R D Levine, F Remacle, E Collini
Elucidating the role of quantum coherences in energy migration within biological and artificial multichromophoric antenna systems is the subject of an intense debate. It is also a practical matter because of the decisive implications for understanding the biological processes and engineering artificial materials for solar energy harvesting. A supramolecular rhodamine heterodimer on a DNA scaffold was suitably engineered to mimic the basic donor-acceptor unit of light-harvesting antennas. Ultrafast 2D electronic spectroscopic measurements allowed identifying clear features attributable to a coherent superposition of dimer electronic and vibrational states contributing to the coherent electronic charge beating between the donor and the acceptor...
August 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28816826/tissue-engineered-bone-differentiated-from-human-adipose-derived-stem-cells-inhibit-posterolateral-fusion-in-an-athymic-rat-model
#7
Comron Saifi, Jonahtan Bernhard, Jamal N Shillingford, Petros Petridis, Samuel Robinson, X Edward Guo, Mark Weidenbaum, Ronald A Lehman, Howard S An, Lawrence G Lenke, Gordana Vunjak-Novakovic, Joseph L Laratta
STUDY DESIGN: Biological augmentation spinal arthrodesis trial in athymic rats OBJECTIVE.: To assess the efficacy of tissue-engineered bone to promote L4-L5 intertransverse process fusion in an athymic rat model. SUMMARY OF BACKGROUND DATA: Each year in the United States, over 400,000 spinal fusion surgeries are performed requiring bone graft. The current gold standard for posterolateral lumbar fusion is autogenous iliac crest bone graft (ICBG), but the harvesting of ICBG is associated with increased operative time and significant complications...
August 14, 2017: Spine
https://www.readbyqxmd.com/read/28816451/palladium-catalyzed-cascade-assembly-of-tricyclic-spiroethers-from-diene-alcohol-precursors
#8
David Lankri, Yelena Mostinski, Dmitry Tsvelikhovsky
Palladium-catalyzed carboetherification-Heck reactions to form tricyclic spiroethers, which are frequently observed as scaffold segments of various biochemical compounds, from simple diene-alcohols have been carried out in a cascade fashion. This is the first attempt to link simple alcohols with diverse, medium-sized spiroether architectures. The reported synthetic strategy is short, robust, and offers rapid delivery of a broad spectrum of tricyclic spiranoid ethers.
August 17, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28816449/paralog-specificity-determines-subcellular-distribution-action-mechanism-and-anticancer-activity-of-trap1-inhibitors
#9
Hye-Kyung Park, Hanbin Jeong, Eunhwa Ko, Geumwoo Lee, Ji-Eun Lee, Sang Kwang Lee, An-Jung Lee, Jin Young Im, Sung Hu, Seong Heon Kim, Ji Hoon Lee, Changwook Lee, Soosung Kang, Byoung Heon Kang
Although Hsp90 inhibitors can inhibit multiple tumorigenic pathways in cancer cells, their anticancer activity has been disappointingly modest. However, by forcing Hsp90 inhibitors into the mitochondria with mitochondrial delivery vehicles, they were converted into potent drugs targeting the mitochondrial Hsp90 paralog TRAP1. Here, to improve mitochondrial drug accumulation without using the mitochondrial delivery vehicle, we increased freely available drug concentrations in the cytoplasm by reducing the binding of the drugs to the abundant cytoplasmic Hsp90...
August 17, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28816404/multivalency-increases-the-binding-strength-of-rgd-peptidomimetic-paclitaxel-conjugates-to-integrin-%C3%AE-v%C3%AE-3
#10
Cesare Gennari, André Raposo Moreira Dias, Arianna Pina, Alberto Dal Corso, Daniela Arosio, Laura Belvisi, Luca Pignataro, Michele Caruso
Herein we report the synthesis of three multimeric RGD peptidomimetic-paclitaxel conjugates featuring a number of αVβ3 integrin ligands ranging from 2 to 4 (compounds 7-9). These constructs were assembled by conjugation of the integrin αVβ3 ligand cyclo[DKP-RGD]-CH2NH2 (2) with paclitaxel (3) via a 2'-carbamate with a self-immolative spacer, the lysosomally cleavable Val-Ala dipeptide linker, a multimeric scaffold, a triazole linkage, and finally a PEG spacer. Two monomeric conjugates (compounds 5-6) were also synthesized as reference compounds...
August 17, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28816316/hydrogel-scaffolds-for-differentiation-of-adipose-derived-stem-cells
#11
REVIEW
Qiutong Huang, Yijie Zou, Maria C Arno, Shuai Chen, Tao Wang, Jingyi Gao, Andrew P Dove, Jianzhong Du
Natural extracellular matrices (ECMs) have been widely used as a support for the adhesion, migration, differentiation, and proliferation of adipose-derived stem cells (ADSCs). However, poor mechanical behavior and unpredictable biodegradation properties of natural ECMs considerably limit their potential for bioapplications and raise the need for different, synthetic scaffolds. Hydrogels are regarded as the most promising alternative materials as a consequence of their excellent swelling properties and their resemblance to soft tissues...
August 17, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28816097/meltblown-polymer-fabrics-as-candidate-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#12
Thomas Jenkins, Sean Meehan, Behnam Pourdeyhimi, Dianne Little
Various biomaterial technologies are promising for tissue engineering, including electrospinning, but commercial scale-up of throughput is difficult. The goal of the study was to evaluate meltblown fabrics as candidate scaffolds for rotator cuff tendon tissue engineering. Meltblown poly(lactic acid) fabrics were produced with several polymer crystallinities and airflow velocities (500(low), 900(medium) or 1400(high) m<sup>3</sup>air/hour/m fabric). Fiber diameter, alignment, and baseline bi-directional tensile mechanical properties were evaluated...
August 17, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28816087/small-diameter-vascular-graft-with-fibroblast-cells-and-electrospun-poly-l-lactide-co-%C3%AE%C2%B5-caprolactone-scaffolds-cell-matrix-engineering
#13
Bong Seok Jang, Ja Young Cheon, Soo Hyun Kim, Won Ho Park
Electrospun scaffolds have been widely used in tissue engineering due to their similar structure to native extracellular matrices (ECM). However, one of the obstacles limiting the application of electrospun scaffolds for tissue engineering is the nano-sized pores, which inhibit cell infiltration into the scaffolds. To overcome this limitation, we approached to make layers which are consisted of cells onto the electrospun sheet and then tubular structure was constructed by rolling. We called this as "Cell Matrix Engineering" because the electrospun sheets were combined with the cells to form one matrix...
August 17, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28816016/the-discovery-of-new-and-more-potent-chloropyramine-c4-analogues-for-the-potential-treatment-of-invasive-breast-cancer
#14
Sahar Kandil, Filippo Prencipe, Samuel Jones, Stephen Hiscox, Andrew D Westwell
Breast cancer (BC) is the second most common cancer worldwide, accounting for 25% of all female cancers. Although the survival rate has increased significantly in the past few decades, patients who develop secondary site metastasis as well as those diagnosed with triple negative breast cancer (TNBC) still represent a real unmet medical challenge. Previous studies have shown that chloropyramine (C4) inhibits FAK-VEGFR3 signaling. More recently, C4 is reported to have SASH1 inducing properties. However, C4 exerts its antitumour and antiangiogenic effects at high micromolar concentrations (>100 μM) that would not be compatible with further drug development against invasive breast cancer driven by FAK signaling...
August 16, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28815883/wwc2-is-an-independent-prognostic-factor-and-prevents-invasion-via-hippo-signalling-in-hepatocellular-carcinoma
#15
Yijun Zhang, Shumei Yan, Jiewei Chen, Caixia Gan, Dong Chen, Yan Li, Jiahuai Wen, Joachim Kremerskothen, Shilu Chen, Jiangbo Zhang, Yun Cao
WWC family proteins negatively regulate HEK293 cell proliferation and organ growth by suppressing the transcriptional activity of Yes-associated protein (YAP), a major effector of the Hippo pathway. The function of the scaffolding protein WWC1 (also called KIBRA) has been intensively studied in cells and animal models. However, the expression and clinicopathologic significance of WWC2 in cancer are poorly characterized. This study aimed to clarify the biological function and mechanism of action of WWC2 in hepatocellular carcinoma (HCC)...
August 16, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28815836/-2-2-paracyclophanes-with-n-heterocycles-as-ligands-for-mono-and-dinuclear-ruthenium-ii-complexes
#16
Carolin Braun, Martin Nieger, Werner R Thiel, Stefan Bräse
The [2.2]paracyclophane with its unique structure allows the design of unusual 3D structures by functionalization of this rigid and stable hydrocarbon scaffold. Therefore different mono- and homodisubstituted [2.2]paracyclophanes with pyridyl, pyrimidyl and oxazolinyl substituents were developed in order to evaluate their ability as bridging ligands for two ruthenium centres. With the successfully synthesized [2.2]paracyclophane-based N-donor functions, the cycloruthenation reaction using [RuCl2(p-cymene)]2 as precursor was explored...
August 16, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28815538/long-noncoding-rnas-in-the-yeast-s-%C3%A2-cerevisiae
#17
Rachel O Niederer, Evan P Hass, David C Zappulla
Long noncoding RNAs have recently been discovered to comprise a sizeable fraction of the RNA World. The scope of their functions, physical organization, and disease relevance remain in the early stages of characterization. Although many thousands of lncRNA transcripts recently have been found to emanate from the expansive DNA between protein-coding genes in animals, there are also hundreds that have been found in simple eukaryotes. Furthermore, lncRNAs have been found in the bacterial and archaeal branches of the tree of life, suggesting they are ubiquitous...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28815506/preparing-fosmid-mate-paired-libraries-using-cre-loxp-recombination
#18
Ze Peng, Jeff L Froula, Jan-Fang Cheng
Fosmid end sequencing has been widely utilized in genome sequence assemblies and genome structural variation studies. We have developed a new approach to construct fosmid paired-end libraries that is suitable for Illumina sequencing platform. This approach employs a newly modified fosmid vector (pFosClip) which contains two loxP sites with identical orientation and two inverse Illumina adaptor priming sites flanking the cloning site. DNA prepared from the fosmid library constructed with pFosClip can be treated with the Cre recombinase to remove most of the vector DNA, leaving only 107 bp of the vector sequence with insert DNA...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28815330/homer1a-protein-expression-in-schizophrenia-bipolar-disorder-and-major-depression
#19
Stefan L Leber, Ida C Llenos, Christine L Miller, Jeannette R Dulay, Johannes Haybaeck, Serge Weis
In recent years, there was growing interest in postsynaptic density proteins in the central nervous system. Of the most important candidates of this specialized region are proteins belonging to the Homer protein family. This family of scaffolding proteins is suspected to participate in the pathogenesis of a variety of diseases. The present study aims to compare Homer1a expression in the hippocampus and cingulate gyrus of patients with major psychiatric disorders including schizophrenia, bipolar disorder and major depression...
August 16, 2017: Journal of Neural Transmission
https://www.readbyqxmd.com/read/28815320/inhibitory-growth-evaluation-and-apoptosis-induction-in-mcf-7-cancer-cells-by-new-5-aryl-2-butylthio-1-3-4-oxadiazole-derivatives
#20
Rashmin Khanam, Kamal Ahmad, Iram I Hejazi, Ibrar A Siddique, Vikash Kumar, Abdul Roouf Bhat, Amir Azam, Fareeda Athar
BACKGROUND: Cancer has become one of the global health issues and it is the life-threatening disease characterized by unrestrained growth of cells. Despite various advances being adopted by chemotherapeutic management, the use of the current anticancer drugs such as Doxorubicin, Asparginase, Methotrexate, Vincristine remains limited due to high toxicity, side effects and developing drug resistance. Apoptosis is a crucial cellular process and improper regulation of apoptotic signaling pathways may lead to cancer formation...
August 16, 2017: Cancer Chemotherapy and Pharmacology
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