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Petra Radulović, Božo Krušlin
Extensive research is being conducted to identify novel diagnostic, predictive and prognostic biomarkers for pancreatic ductal adenocarcinoma (PDAC), as only a few markers have been routinely used so far with limited success. Our aim was to assess the expression of neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), E-cadherin, and γ-catenin in PDAC in relation to clinicopathological parameters and patient survival. We also investigated if there is a correlation of NEDD9 expression with E-cadherin or γ-catenin...
June 20, 2018: Bosnian Journal of Basic Medical Sciences
Eric A Miller, Yara Jabbour Al Maalouf, Hadley D Sikes
In this work, we characterize the impact of large-volume processing upon the analytical sensitivity of flow-through paper-based immunoassays. Larger sample volumes feature greater molar quantities of available analyte, but the assay design principles which would enable the rapid collection of this dilute target are ill-defined. We developed a finite-element model to explore the operat-ing conditions under which processing large sample volumes via pressure-driven convective flow would yield improved binding signal...
June 20, 2018: Analytical Chemistry
Courtney A Miller, Geraud Canis Tasse Taboue, Mary M P Ekane, Matthew Robak, Paul R Sesink Clee, Corinne Richards-Zawacki, Eric B Fokam, Nkwatoh Athanasius Fuashi, Nicola M Anthony
The amphibian disease chytridiomycosis in amphibians is caused by the chytrid fungus Batrachochytrium dendrobatidis (Bd) and has resulted in dramatic declines and extinctions of amphibian populations worldwide. A hypervirulent, globally-dispersed pandemic lineage (Bd-GPL) is thought to be largely responsible for population declines and extinctions, although numerous endemic lineages have also been found. Recent reports of amphibian declines have been linked to the emergence of Bd in Cameroon, a major hotspot of African amphibian diversity...
2018: PloS One
Laura Flatau, Markus Reitt, Gunnar Duttge, Christian Lenk, Barbara Zoll, Wolfgang Poser, Alexandra Weber, Urs Heilbronner, Marcella Rietschel, Jana Strohmaier, Rebekka Kesberg, Jonas Nagel, Thomas G Schulze
In clinical practice and in research, there is an ongoing debate on how to return incidental and secondary findings of genetic tests to patients and research participants. Previous investigations have found that most of the people most of the time are in favor of full disclosure of results. Yet, the option to reject disclosure, based on the so-called right not to know, can be valuable especially for some vulnerable subgroups of recipients. In the present study we investigated variations in informational preferences in the context of genetic testing in a large and diverse German sample...
2018: PloS One
Eric S Kilpatrick, Sverre Sandberg
The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) has initiated many harmonization activities in all phases of the examination process. The EFLM is dealing with both the scientific and the educational aspects of harmonization, with the intention of disseminating best practice in laboratory medicine throughout Europe. Priorities have been given (1) to establish a standard for conducting and assessing biological variation studies and to construct an evidence based EFLM webpage on biological variation data, (2) to harmonize preanalytical procedures by producing European guidelines, (3) to improve test ordering and interpretation, (4) to produce other common European guidelines for laboratory medicine and play an active part in development of clinical guidelines, (5) to establish a common basis for communicating laboratory results to patients, (6) to harmonize units of measurement throughout Europe, (7) to harmonize preanalytical procedures in molecular diagnostics and (8) to harmonize and optimize test evaluation procedures...
June 20, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Yuting Xiu, Baosheng Sun, Yongli Jiang, Aifu Wang, Linlin Liu, Ying Liu, Shilong Sun, Mingmei Huangfu
BACKGROUND: Tumor-associated antigen overexpression, which has been reported in many types of cancers, may trigger autoantibody secretion. The present study was designed to test whether levels of circulating autoantibodies to survivin protein-derived antigens is altered in liver, esophageal, breast, and lung cancers. METHODS: Patients with liver (144), esophageal (159), breast (124), and lung cancers (267), and healthy volunteers (362) were recruited for the study, and serum samples were collected for ELISA autoantibody analysis...
June 2018: Genetic Testing and Molecular Biomarkers
P Horner, G Donders, M Cusini, M Gomberg, J S Jensen, M Unemo
At present, we have no evidence that we are doing more good than harm detecting and subsequently treating Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum colonisations/infections. Consequently, routine testing and treatment of asymptomatic or symptomatic men and women for M. hominis, U. urealyticum, and U. parvum is not recommended. Asymptomatic carriage of these bacteria is common and the majority of individuals do not develop disease. Although U. urealyticum has been associated with urethritis in men, it is probably not causal unless a high load is present (likely carriage in 40-80% of detected cases)...
June 20, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
Renhua Wu, Nanwei Tong, Xinlei Chen, Shishi Xu, Fang Zhang, Lizhi Tang, Yuwei Zhang
RATIONALE: Pheochromocytomas are rare catecholamine-secreting tumors arising from adrenomedullary chromaffin cells, usually causing hypertension, palpitation and headache. However, pheochromocytoma crisis, on the contrary, might present with hypotension, multiple organ dysfunction or even mimicking other diseases, leaving physician with diagnostic difficulties. In this study, we present a case featured hypotension, shock and multiple organ dysfunction syndrome on admission, which nearly lead us to miss the diagnosis of pheochromocytoma...
June 2018: Medicine (Baltimore)
Marie E Robert, Sheila E Crowe, Lawrence Burgart, Rhonda K Yantiss, Benjamin Lebwohl, Joel K Greenson, Stefano Guandalini, Joseph A Murray
Small intestinal biopsy interpretation has been the cornerstone for the diagnosis of celiac disease for over 50 years. Despite the existence of sensitive and specific serological tests, duodenal mucosal biopsies continue to be obtained in the vast majority of patients in whom a diagnosis of celiac disease is being considered. The accurate evaluation of these biopsies requires coordination and information sharing between the gastroenterologist, laboratory, and pathologist in order to optimize tissue sampling, preparation and interpretation...
June 19, 2018: American Journal of Surgical Pathology
Jyoti S Kumar, Divyasha Saxena, Manmohan Parida, Sivakumar Rathinam
Background & objectives: West Nile virus (WNV) is a mosquito-borne flavivirus. The disease can be diagnosed by isolation followed by fluorescent antibody tests, enzyme-linked immunosorbent assay and polymerase chain reaction (PCR) assay. These diagnostic methods are laborious and time-consuming. The present study was aimed to evaluate the real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, early and accurate diagnosis of WNV. Methods: A one-step single tube accelerated quantitative RT-LAMP assay was evaluated by targeting the Env gene of WNV...
March 2018: Indian Journal of Medical Research
Michał Bieńkowski, Adelheid Wöhrer, Patrizia Moser, Melitta Kitzwögerer, Gerda Ricken, Thomas Ströbel, Johannes A Hainfellner
Typing of diffuse gliomas according to the WHO 2016 Classification of Tumors of the Central Nervous System is based on the integration of histology with molecular biomarkers. However, the choice of appropriate methods for molecular analysis and criteria for interpretation of test results is left to each diagnostic laboratory. In the present study, we tested the applicability of combined immunohistochemistry, direct sequencing, and multiplex ligation-dependent probe amplification (MLPA) for diagnostic assessment of <i>IDH1/2</i> mutation status, chromosome 1p/19q status, and <i>TERT</i> promoter mutations...
June 20, 2018: Clinical Neuropathology
Aude Paquet, Bertrand Olliac, Bernard Golse, Laurence Vaivre-Douret
INTRODUCTION: Several authors have suggested the existence of motor disorders associated with developmental coordination disorder (DCD) in individuals with autism spectrum disorder (ASD). However, there are few comparative studies of psychomotor profiles that include assessments of neurological soft signs in children with ASD or DCD. We used a neuropsychomotor assessment for children with ASD from a standardized neurodevelopmental examination to understand the nature of the difficulties these children encounter...
June 20, 2018: Journal of Clinical and Experimental Neuropsychology
Tim R de Back, Arnon P Kater, Sanne H Tonino
Chronic lymphocytic leukemia (CLL) is frequently complicated by cytopenias, either due to bone marrow infiltration or autoimmunity, resulting in autoimmune hemolytic anemia (AIHA), immune thrombocytopenia (ITP), pure red cell aplasia (PRCA) or autoimmune neutropenia (AIN). Morbidity due to autoimmune cytopenias (AIC) can be substantial; in addition, infection risk increases and pre-existing infections might deteriorate due to immunosuppressive medication. In the aging population, CLL occurs more frequently and AIC related to CLL represent a growing clinical challenge...
June 20, 2018: Expert Review of Hematology
P Behm, M Gastl, A Jahn, A Rohde, S Haberkorn, S Krueger, S Weiss, B Schnackenburg, M Sager, K Düring, H Clogenson, P Horn, R Westenfeld, M Kelm, M Neizel-Wittke, F Bönner
Endomyocardial biopsy (EMB) is considered to be the diagnostic gold-standard in detection of myocardial-inflammation. EMB is usually conducted under fluoroscopy without any specific target information. Specific target-information provided by cardiovascular magnetic resonance (CMR) may improve specificity of EMB. The aim was to investigate feasibility and safety of CMR-guided and targeted EMB in a preclinical-model using passively-tracked devices. Procedures were performed on a MRI-System equipped with an Interventional Software-Platform for real-time imaging...
June 19, 2018: International Journal of Cardiovascular Imaging
Giovanni Foti, Giuseppe Malleo, Niccolò Faccioli, Andrea Guerriero, Lino Furlani, Giovanni Carbognin
PURPOSE: To evaluate the diagnostic accuracy of wash-out parameters calculated using multiple intermediate and delayed phases. MATERIALS AND METHODS: This prospective study had institutional review board approval and informed consent was obtained from all patients. Between January 2012 and October 2016, 108 consecutive oncologic patients (59 males, 49 females, mean age 52.6 years; 129 diagnosed lesions) underwent multiphasic CT protocol including unenhanced (UE), arterial (AE), portal (PE), 5-min (DE-5) and the 15-min (DE-15) delayed phases of adrenal glands...
June 19, 2018: La Radiologia Medica
Laura Delgado-Ortega, Almudena González-Domínguez, Josep María Borrás, Juan Oliva-Moreno, Eva González-Haba, Salomón Menjón, Pedro Pérez, David Vicente, Luis Cordero, Margarita Jiménez, Susana Simón, Álvaro Hidalgo-Vega, Carlota Moya-Alarcón
OBJECTIVE: To assess the economic burden of epithelial ovarian cancer (EOC) in incident patients and the burden by disease stage in Spain. METHODS: We developed a Markov model from a social perspective simulating the natural history of EOC and its four stages, with a 10-year time horizon, 3-week cycles, 3% discount rate, and 2016 euros. Healthcare resource utilization and costs were estimated by disease stage. Direct healthcare costs (DHC) included early screening, genetic counselling, medical visits, diagnostic tests, surgery, chemotherapy, hospitalizations, emergency services, and palliative care...
June 19, 2018: European Journal of Health Economics: HEPAC: Health Economics in Prevention and Care
Shyam Sundar, Om Prakash Singh
Visceral leishmaniasis (VL), a deadly parasitic disease, is a major public health concern globally. Countries affected by VL have signed the London Declaration on Neglected Tropical Diseases and committed to eliminate VL as a public health problem by 2020. To achieve and sustain VL elimination, it will become progressively important not to miss any remaining cases in the community who can maintain transmission. This requires accurate identification of symptomatic and asymptomatic carriers using highly sensitive diagnostic tools at the primary health service setting...
June 19, 2018: Molecular Diagnosis & Therapy
C C A H Bours, M J Bakker-Huvenaars, J Tramper, N Bielczyk, F Scheepers, K S Nijhof, A N Baanders, N N J Lambregts-Rommelse, P Medendorp, J C Glennon, J K Buitelaar
Autism Spectrum Disorder (ASD), Oppositional Defiant Disorder (ODD), and Conduct Disorder (CD) are often associated with emotion recognition difficulties. This is the first eye-tracking study to examine emotional face recognition (i.e., gazing behavior) in a direct comparison of male adolescents with Autism Spectrum Disorder or Oppositional Defiant Disorder/Conduct Disorder, and typically developing (TD) individuals. We also investigate the role of psychopathic traits, callous-unemotional (CU) traits, and subtypes of aggressive behavior in emotional face recognition...
June 19, 2018: European Child & Adolescent Psychiatry
Mostafa Rad, Marzieh Torkmannejad Sabzevary, Zahra Mohebbi Dehnavi
BACKGROUND: Premenstrual syndrome (PMS) is the emergence of periodic one or more symptoms of symptoms before menstruation and in the first few days of menstruation. Lifestyle is one of the series of factors that affect the health of people. Activity, smoking, and food intake are factors associated with lifestyle, and evidence suggests that women and girls with PMS do not have an adequate life span. The aim of this study was to determine the factors associated with PMS in female high school students...
2018: Journal of Education and Health Promotion
Rebecca Sanders, Stephen Bustin, Jim Huggett, Deborah Mason
Human health and safety depend on reliable measurements in medical diagnosis and on tests that support the selection and evaluation of therapeutic intervention and newly discovered molecular biomarkers must pass a rigorous evaluation process if they are to be of benefit to patients. Measurement standardization helps to maximize data quality and confidence and ultimately improves the reproducibility of published research. Failure to consider how a given experiment may be standardized can be costly, both financially as well as in time and failure to perform and report pre-clinical research in an appropriately rigorous manner will hinder the development of diagnostic methods...
May 2018: Biomolecular Detection and Quantification
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