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https://www.readbyqxmd.com/read/28239963/characterization-of-complex-heterogeneous-lipid-a-samples-using-hplc-ms-ms-technique-ii-structural-elucidation-of-non-phosphorylated-lipid-a-by-negative-ion-mode-tandem-mass-spectrometry
#1
Viktor Sándor, Anikó Kilár, Ferenc Kilár, Béla Kocsis, Ágnes Dörnyei
Non-phosphorylated lipid A species confer reduced inflammatory potential for the bacteria. Knowledge on their chemical structure and presence in bacterial pathogens may contribute to the understanding of bacterial resistance and activation of the host innate immune system. In this study, we report the fragmentation pathways of negatively charged, non-phosphorylated lipid A species under low-energy collision-induced dissociation conditions of an electrospray ionization quadrupole time-of-flight instrument. Charge-promoted consecutive and competitive eliminations of the acyl chains and cross-ring cleavages of the sugar residues were observed...
August 2016: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28239838/which-matters-more-a-retrospective-cohort-study-of-headache-characteristics-and-diagnosis-type-in-soldiers-with-mtbi-concussion
#2
Alan G Finkel, Brian J Ivins, Juanita A Yerry, John S Klaric, Ann Scher, Y Sammy Choi
OBJECTIVE: To describe the diagnostic types and characteristics of headaches in soldiers with mild traumatic brain injury during the wars in Afghanistan and Iraq. BACKGROUND: Persistent post-traumatic headache interferes with returns to activity or duty. The most commonly cited headache diagnosis after concussion is migraine. We hypothesize that headache diagnosis type, eg, migraine, is not sufficient to predict relationships with occupational outcomes after concussion...
February 27, 2017: Headache
https://www.readbyqxmd.com/read/28239797/characteristics-of-multipotent-mesenchymal-stromal-cells-isolated-from-the-endometrium-and-endometriosis-lesions-of-women-with-malformations-of-the-internal-reproductive-organs
#3
A M Savilova, K N Farkhat, M N Yushina, Yu V Rudimova, Z N Makiyan, L V Adamyan
We isolated and characterized cell cultures from eutopic endometrium and endometriotic lesions of women with malformations of the internal reproductive organs. The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. The obtained cultures exhibited properties of multipotent mesenchymal stromal cells; at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference...
February 27, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28239639/alzheimer-s-biomarkers-in-daily-practice-abide-project-rationale-and-design
#4
Arno de Wilde, Ingrid S van Maurik, Marleen Kunneman, Femke Bouwman, Marissa Zwan, Eline A J Willemse, Geert Jan Biessels, Mirella Minkman, Ruth Pel, Niki S M Schoonenboom, Ellen M A Smets, Mike P Wattjes, Frederik Barkhof, Andrew Stephens, Erik J van Lier, Richard Batrla-Utermann, Philip Scheltens, Charlotte E Teunissen, Bart N M van Berckel, Wiesje M van der Flier
INTRODUCTION: The Alzheimer's biomarkers in daily practice (ABIDE) project is designed to translate knowledge on diagnostic tests (magnetic resonance imaging [MRI], cerebrospinal fluid [CSF], and amyloid positron emission tomography [PET]) to daily clinical practice with a focus on mild cognitive impairment (MCI). METHODS: ABIDE is a 3-year project with a multifaceted design and is structured into interconnected substudies using both quantitative and qualitative research methods...
2017: Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring
https://www.readbyqxmd.com/read/28239553/on-the-use-of-an-optoacoustic-and-laser-ultrasonic-imaging-system-for-assessing-peripheral-intravenous-access
#5
A S Bychkov, V P Zarubin, A A Karabutov, V A Simonova, E B Cherepetskaya
We describe a universal system for research in combined real-time optoacoustic (OA) and laser-ultrasonic (LU) imaging. The results of its testing on the task of needle insertion into the blood vessel model diagnostics are presented. In OA mode, where laser light is absorbed directly in the sample, the contents of blood vessel model is clearly visible. In LU mode, where the short ultrasonic probe pulse scattered on the sample is detected, the needle is clearly visible. The developed solution combining OA and LU imaging modalities due to the common detection system allowed real-time diagnostics of the position of medical needles (0...
March 2017: Photoacoustics
https://www.readbyqxmd.com/read/28239513/rapid-molecular-detection-of-zika-virus-in-acute-phase-urine-samples-using-the-recombinase-polymerase-amplification-assay
#6
Ahmed Abd El Wahed, Sabri Saeed Sanabani, Oumar Faye, Rodrigo Pessôa, João Veras Patriota, Ricardo Rodrigues Giorgi, Pranav Patel, Susanne Böhlken-Fascher, Olfert Landt, Matthias Niedrig, Paolo Marinho de Andrade Zanotto, Claus Peter Czerny, Amadou A Sall, Manfred Weidmann
BACKGROUND: Currently the detection of Zika virus (ZIKV) in patient samples is done by real-time RT-PCR. Samples collected from rural area are sent to highly equipped laboratories for screening. A rapid point-of-care test is needed to detect the virus, especially at low resource settings. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we describe the development of a reverse transcription isothermal recombinase polymerase amplification (RT-RPA) assay for the identification of ZIKV...
January 25, 2017: PLoS Currents
https://www.readbyqxmd.com/read/28239509/system-design-and-development-of-a-robotic-device-for-automated-venipuncture-and-diagnostic-blood-cell-analysis
#7
Max L Balter, Alvin I Chen, Alex Fromholtz, Alex Gorshkov, Tim J Maguire, Martin L Yarmush
Diagnostic blood testing is the most prevalent medical procedure performed in the world and forms the cornerstone of modern health care delivery. Yet blood tests are still predominantly carried out in centralized labs using large-volume samples acquired by manual venipuncture, and no end-to-end solution from blood draw to sample analysis exists today. Our group is developing a platform device that merges robotic phlebotomy with automated diagnostics to rapidly deliver patient information at the site of the blood draw...
October 2016: Proceedings of the ... IEEE/RSJ International Conference on Intelligent Robots and Systems
https://www.readbyqxmd.com/read/28239362/reducing-the-diagnostic-heterogeneity-of-schizoaffective-disorder
#8
Katherine Seldin, Kristan Armstrong, Max L Schiff, Stephan Heckers
OBJECTIVE: Clinical outcome studies of schizoaffective disorder patients have yielded conflicting results. One reason is the heterogeneity of samples drawn from the schizoaffective disorder population. Here, we studied schizoaffective disorder patients who showed marked functional impairment and continuous signs of illness for at least 6 months (i.e., DSM criteria B and C for schizophrenia). METHODS: We assessed 176 chronic psychosis patients with a structured interview (SCID-IV-TR) and the Diagnostic Interview for Genetic Studies schizoaffective disorder module...
2017: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/28239223/exercise-reduces-depressive-symptoms-in-adults-with-arthritis-evidential-value
#9
George A Kelley, Kristi S Kelley
PURPOSE: Determine whether evidential value exists that exercise reduces depressive symptoms in adults with arthritis and other rheumatic diseases. METHODS: Using data from a previous meta-analysis of 29 published studies that included 2449 participants (1470 exercise, 979 control) with fibromyalgia, osteoarthritis, rheumatoid arthritis or systemic lupus erythematosus, a novel, recently developed method, p-curve, was used to assess for evidential value and rule out selective reporting of statistically significant findings regarding exercise and depressive symptoms in adults with arthritis and other rheumatic diseases...
May 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28239077/a-review-of-diagnosis-of-gerd
#10
REVIEW
In Du Jeong
The prevalence of gastroesophageal reflux disease (GERD) and the incidence of some of its complications have risen strikingly over the last few decades. With the accumulation in our understanding on the pathophysiology of GERD along with the development of proton pump inhibitors, the diagnostic and therapeutic approaches to the GERD have changed dramatically over the past decade. However, The GERD still poses a problem to many clinicians since the spectrum of the disease has evolved to encompass more challenging presentations such as refractory GERD and extraesophageal manifestations...
February 25, 2017: Korean Journal of Gastroenterology, Taehan Sohwagi Hakhoe Chi
https://www.readbyqxmd.com/read/28238695/how-expert-clinicians-intuitively-recognize-a-medical-diagnosis
#11
REVIEW
John E Brush, Jonathan Sherbino, Geoffrey R Norman
Research has shown that expert clinicians make a medical diagnosis through a process of hypothesis generation and verification. Experts begin the diagnostic process by generating a list of diagnostic hypotheses using intuitive, non-analytical reasoning. Analytical reasoning then allows the clinician to test and verify or reject each hypothesis, leading to a diagnostic conclusion. In this paper, we focus on the initial step of hypothesis generation and review how expert clinicians use experiential knowledge to intuitively recognize a medical diagnosis...
February 24, 2017: American Journal of Medicine
https://www.readbyqxmd.com/read/28238505/rapid-diagnosis-of-sexually-transmitted-infections
#12
Luis Otero-Guerra, Ana Fernández-Blázquez, Fernando Vazquez
Sexually transmitted infections (STIs) are responsible for an enormous burden of morbidity and mortality. Worldwide, millions of cases of STIs, such as syphilis, chlamydia, or gonorrhoea occur every year, and there is now an increase in antimicrobial resistance in pathogens, such as gonococcus. Delay in diagnosis is one of the factors that justifies the difficulty in controlling these infections. Rapid diagnostic tests allow the introduction of aetiological treatment at the first visit, and also leads to treating symptomatic and asymptomatic patients more effectively, as well as to interrupt the epidemiological transmission chain without delay...
February 23, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28238460/-irritable-bowel-syndrome-with-constipation-and-functional-constipation-in-adults-treatment-part-2-of-2
#13
F Mearin, C Ciriza, M Mínguez, E Rey, J J Mascort, E Peña, P Cañones, J Júdez
In this Clinical practice guide we examine the diagnostic and therapeutic management of adult patients with constipation and abdominal discomfort, at the confluence of the spectrum of irritable bowel syndrome and functional constipation. Both fall within the framework of functional intestinal disorders and have major personal, health and social impact, altering the quality of life of the patients affected. The former is a subtype of irritable bowel syndrome in which constipation and altered bowel habit predominate, often along with recurring abdominal pain, bloating and abdominal distension...
February 23, 2017: Atencion Primaria
https://www.readbyqxmd.com/read/28238388/multicenter-evaluation-of-the-revised-rida%C3%A2-quick-test-n1402-for-rapid-detection-of-norovirus-in-a-diagnostic-laboratory-setting
#14
Stijn Jonckheere, Nadine Botteldoorn, Patricia Vandecandelaere, Johan Frans, Wim Laffut, Guy Coppens, Anne Vankeerberghen, Hans De Beenhouwer
The updated RIDA® QUICK (N1402) immunochromatographic assay (R-Biopharm) for detection of norovirus was evaluated during a prospective, multicenter study using 771 stool samples from patients with gastroenteritis. Compared to real-time reverse transcriptase polymerase chain reaction (RT-rtPCR) as gold standard, the RIDA® QUICK had an overall sensitivity of 72.8% (91/125) and a specificity of 99.5% (640/643). Genotype analysis of the polymerase (ORF1) and capsid (ORF2) region of the genome indicated that the RIDA® QUICK assay could detect a broad range of genotypes including new variants (15 of 125 positive samples) which were detected by an in-house SYBR®Green RT-rtPCR, but not by the RIDA® GENE PCR PG1415 (R-Biopharm) and mostly not by the RIDA® GENE PCR PG1405 and the Xpert® Norovirus assay (Cepheid)...
February 12, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28238286/a-13-year-old-girl-with-pancytopenia-at-the-presentation-of-a-borrelia-hispanica-infection-a-case-report-and-review-of-the-literature
#15
Irmin Leen, Peggy Bruynseels, Benoît Kabamba Mukadi, Mark van Oort, Machiel van den Akker
BACKGROUND: It is not uncommon that a child with a febrile illness of unknown etiology is admitted to the hospital. When the complete blood count reveals a pancytopenia, the diagnostic process can be a real challenge. CASE PRESENTATION: A 13-year girl of Arab-Berber descent presented with abdominal pain and fever after a holiday in northwestern Morocco. A complete blood count revealed a pancytopenia and blood smear test results revealed spirochetes. Borrelia hispanica was identified by sequencing the 16S ribosomal ribonucleic acid gene...
February 27, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28238272/psychiatric-presentations-of-c9orf72-mutation-what-are-the-diagnostic-implications-for-clinicians
#16
Simon Ducharme, Sepideh Bajestan, Bradford C Dickerson, Valerie Voon
The C9orf72 mutation was identified as the most frequent genetic cause of frontotemporal dementia (FTD). In light of multiple reports of predominant psychiatric presentations of FTD secondary to C9orf72 mutation, the American Neuropsychiatric Association Committee on Research reviewed all studies on psychiatric aspects of this mutation to identify clinically relevant features for diagnosis. The most common psychiatric presentation is psychosis (21%-56%), with delusions, and/or multimodal hallucinations. Other presentations include late-onset mania and depression with cognitive impairment or catatonia...
February 27, 2017: Journal of Neuropsychiatry and Clinical Neurosciences
https://www.readbyqxmd.com/read/28238212/development-a-rapid-and-accurate-multiplex-real-time-pcr-method-for-the-detection-chlamydia-trachomatis-and-mycoplasma-hominis
#17
Roya Safarkar, Jalil Fallah Mehrabadi, Zahra Noormohammadi, Reza Mirnejad
BACKGROUND: Sexually transmitted diseases easily spread among sexually active people and often have no symptoms. Rapid and accurate method for detecting these infections are necessary in early stages. The traditional detection methods of them are difficult and time-consuming. METHODS: In this study, multiplex real time PCR was optimized for rapid identification of Chlamydia trachomatis and Mycoplasma hominis in a single tube and was performed with our designed primers...
February 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28238060/novel-biotechnology-approaches-in-colorectal-cancer-diagnosis-and-therapy
#18
REVIEW
Soudabeh Kavousipour, Fathemeh Khademi, Mozhdeh Zamani, Bahareh Vakili, Pooneh Mokarram
With ever-increasing molecular information about colorectal cancer (CRC), there is an expectation to detect more sensitive and specific molecular markers for new advanced diagnostic methods that can surpass the limitations of current screening tests. Moreover, enhanced molecular pathology knowledge about cancer has led to the development of targeted therapies, designed to interfere with specific aberrant biological pathways in cancer. Furthermore, biotechnology has opened a new window in CRC diagnosis and treatment by introducing different application of antibodies, antibody fragments, non-Ig scaffold proteins, and aptamers in targeted therapy and drug delivery...
February 25, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28237932/effects-of-concurrent-and-delayed-visual-feedback-on-motor-memory-consolidation
#19
Dangxiao Wang, Teng Li, Gaofeng Yang, Yuru Zhang
In many domains, it's important to understand the ways in which humans learn and develop new motor skills effectively and efficiently. For example, in dental operations, the ability to apply a weak force with a required tolerance is a fundamental skill to ensure diagnostic and treatment outcome, but acquiring such a skill is a challenge for novices. In this paper, we focus on motor memory for producing normally applied force by a hand-held probe and we compare the effects of two feedback methods on motor memory consolidation...
February 22, 2017: IEEE Transactions on Haptics
https://www.readbyqxmd.com/read/28237659/production-of-mono-and-polyclonal-antibodies-to-citrus-leprosis-virus-c2-and-their-application-in-triple-antibody-sandwich-elisa-and-immunocapture-rt-pcr-diagnostic-assays
#20
Nandlal Choudhary, Avijit Roy, Leon M G, G Wei, M K Nakhla, L Levy, R H Brlansky
The newly discovered Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) is one of the causal virus of citrus leprosis disease complex; which leads to substantial loss of citrus production in the states of Meta and Casanare of Colombia. Specific and sensitive detection methods are needed to monitor the dissemination of CiLV-C2 in Colombia, and to prevent introduction of CiLV-C2 to other citrus growing countries. Toward this end, putative coat protein gene (CPG) of CiLV-C2 was amplified from CiLV-C2 infected citrus tissues...
February 22, 2017: Journal of Virological Methods
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