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Candida mlst

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https://www.readbyqxmd.com/read/27915298/multilocus-sequence-typing-mlst-analysis-of-candida-albicans-isolates-colonizing-acrylic-dentures-before-and-after-denture-replacement
#1
Khai How Choo, Hee Ji Lee, Nicholas J Knight, Ann R Holmes, Richard D Cannon
Yeast, in particular Candida albicans, are the principal fungal cause of denture stomatitis, and can also be present as a commensal in many individuals. Few studies, however, have examined oral retention of yeast strains over time. We analyzed the yeast present in saliva samples and from the dentures of 10 individuals colonized with yeast but with no signs of stomatitis, before new complete maxillary dentures were fitted and also at 1, 3, and 6 months after denture replacement. Yeast species were presumptively identified on selective agar plates and were present in nine individuals before denture replacement and in six at the 6-month time point...
December 2, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#2
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
November 30, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27855078/echinocandin-resistance-in-candida-spp-isolated-from-liver-transplant-recipients
#3
Gwénolé Prigent, Nawel Aït-Ammar, Eric Levesque, Arnaud Fekkar, Jean-Marc Costa, Sarra El Anbassi, Françoise Foulet, Christophe Duvoux, Jean-Claude Merle, Eric Dannaoui, Françoise Botterel
Liver transplant recipients are at risk of invasive fungal infections, especially candidiasis. Echinocandin is recommended as prophylactic treatment but is increasingly associated with resistance. Our aim was to assess echinocandin drug resistance in Candida spp. isolated from liver transplant recipients treated with this antifungal class. For this, all liver-transplanted patients in a University Hospital (Créteil, France) between January and June of 2013 and 2015 were included. Susceptibilities of Candida isolates to echinocandins were tested by Etest® and EUCAST reference method...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27456280/multilocus-sequence-typing-of-candida-tropicalis-shows-clonal-cluster-enrichment-in-azole-resistant-isolates-from-patients-in-shanghai-china
#4
Ying Wang, Ce Shi, Jin-Yan Liu, Wen-Jing Li, Yue Zhao, Ming-Jie Xiang
To explore the putative correlation between the multilocus sequence types (MLST) and antifungal susceptibility of clinical Candida tropicalis isolates in Mainland China. Eighty-two clinical C. tropicalis isolates were collected from sixty-nine patients at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China, from July 2012 to February 2015, and antifungal susceptibility tests were performed. Genetic profiles of those 82 isolates (30 azole-resistant and 52 azole-susceptible) were characterised by multilocus sequence typing...
October 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/26849050/clonal-strain-persistence-of-candida-albicans-isolates-from-chronic-mucocutaneous-candidiasis-patients
#5
Alexander J Moorhouse, Claire Rennison, Muhammad Raza, Desa Lilic, Neil A R Gow
Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST)...
2016: PloS One
https://www.readbyqxmd.com/read/26848577/comparison-of-e-e-farnesol-secretion-and-the-clinical-characteristics-of-candida-albicans-bloodstream-isolates-from-different-multilocus-sequence-typing-clades
#6
Sook-In Jung, Jong Hee Shin, Soo Hyun Kim, Jin Kim, Joo Hee Kim, Min Ji Choi, Eun-Kyung Chung, Kyungwon Lee, Sun Hoe Koo, Hyun Ha Chang, Marie-Elisabeth Bougnoux, Christophe d'Enfert
Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed...
2016: PloS One
https://www.readbyqxmd.com/read/26711776/posttreatment-antifungal-resistance-among-colonizing-candida-isolates-in-candidemia-patients-results-from-a-systematic-multicenter-study
#7
R H Jensen, H K Johansen, L M Søes, L E Lemming, F S Rosenvinge, L Nielsen, B Olesen, L Kristensen, E Dzajic, K M T Astvad, M C Arendrup
The prevalence of intrinsic and acquired resistance among colonizing Candida isolates from patients after candidemia was investigated systematically in a 1-year nationwide study. Patients were treated at the discretion of the treating physician. Oral swabs were obtained after treatment. Species distributions and MIC data were investigated for blood and posttreatment oral isolates from patients exposed to either azoles or echinocandins for <7 or ≥ 7 days. Species identification was confirmed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and internal transcribed spacer (ITS) sequencing, susceptibility was examined by EUCAST EDef 7...
March 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26587501/molecular-diversity-of-candida-albicans-isolated-from-immunocompromised-patients-based-on-mlst-method
#8
Seyed Mohammad Hosein Afsarian, Hamid Badali, Tahereh Shokohi, Sohrab Najafipour
BACKGROUND: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. METHODS: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients...
September 2015: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/26548511/evidence-of-genotypic-diversity-among-candida-auris-isolates-by-multilocus-sequence-typing-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-amplified-fragment-length-polymorphism
#9
A Prakash, C Sharma, A Singh, P Kumar Singh, A Kumar, F Hagen, N P Govender, A L Colombo, J F Meis, A Chowdhary
Candida auris is a multidrug-resistant nosocomial bloodstream pathogen that has been reported from Asian countries and South Africa. Herein, we studied the population structure and genetic relatedness among 104 global C. auris isolates from India, South Africa and Brazil using multilocus sequence typing (MLST), amplified fragment length polymorphism (AFLP) fingerprinting and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). RPB1, RPB2 and internal transcribed spacer (ITS) and D1/D2 regions of the ribosomal DNA were sequenced for MLST...
March 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/26333357/molecular-epidemiology-of-invasive-candida-albicans-at-a-tertiary-hospital-in-northern-taiwan-from-2003-to-2011
#10
Shao-Hung Wang, Mandy Shen, Hsin-Chieh Lin, Pei-Lun Sun, Hsiu-Jung Lo, Jang-Jih Lu
Candida albicans is a common cause of bloodstream fungal infections in hospitalized patients. To investigate its epidemiology, multilocus sequence typing (MLST) was performed on 285 C. albicans bloodstream isolates from patients in Chang Gung Memorial Hospital at Linkou (CGMHL), Taiwan from 2003 to 2011. Among these isolates, the three major diploid sequence types (DSTs) were 693, 659, and 443 with 19, 16, and 13 isolates, respectively. The 179 DSTs were classified into 16 clades by unweighted pair-group method using arithmetic averages (UPGMA)...
November 2015: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/26324013/candida-albicans-bloodstream-isolates-in-a-german-university-hospital-are-genetically-heterogenous-and-susceptible-to-commonly-used-antifungals
#11
Johanna Huyke, Ronny Martin, Grit Walther, Michael Weber, Kerstin Kaerger, Marie-Elisabeth Bougnoux, Johannes Elias, Oliver Kurzai
From an eight-year-span, 99 Candida bloodstream isolates were collected at the University Hospital Wuerzburg, Germany. In this study, all strains were analyzed using molecular and phenotypic typing methods. Confirmatory species identification revealed three isolates that were initially diagnosed as C. albicans to be actually C. dubliniensis. Two isolates contained a mixed culture of C. albicans and C. glabrata, in one of the specimens both species could be separated while it was not possible to recover C. albicans in the other sample...
October 2015: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/26148466/clinical-and-molecular-characteristics-of-bloodstream-infections-caused-by-candida-albicans-in-children-from-2003-to-2011
#12
M-H Tsai, S-H Wang, J-F Hsu, L-C Lin, S-M Chu, H-R Huang, M-C Chiang, R-H Fu, J-J Lu, Y-C Huang
We investigated the clinical and molecular characteristics of Candida albicans bloodstream infection (BSI) in children from a tertiary-level medical centre in Taiwan over a 9-year period from January 2003 to December 2011. We performed multilocus sequence typing (MLST) to investigate the genetic relatedness of these C. albicans BSI isolates. A total of 79 episodes of C. albicans BSI in 76 paediatric patients were identified, including 41 (51.9%) from the paediatric intensive care unit, 24 (30.4%) from the neonatal intensive care unit and 14 (17...
November 2015: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/26017038/stepwise-emergence-of-azole-echinocandin-and-amphotericin-b-multidrug-resistance-in-vivo-in-candida-albicans-orchestrated-by-multiple-genetic-alterations
#13
Rasmus Hare Jensen, Karen Marie Thyssen Astvad, Luis Vale Silva, Dominique Sanglard, Rene Jørgensen, Kristian Fog Nielsen, Estella Glintborg Mathiasen, Ghazalel Doroudian, David Scott Perlin, Maiken Cavling Arendrup
OBJECTIVES: The objective of this study was to characterize the underlying molecular mechanisms in consecutive clinical Candida albicans isolates from a single patient displaying stepwise-acquired multidrug resistance. METHODS: Nine clinical isolates (P-1 to P-9) were susceptibility tested by EUCAST EDef 7.2 and Etest. P-4, P-5, P-7, P-8 and P-9 were available for further studies. Relatedness was evaluated by MLST. Additional genes were analysed by sequencing (including FKS1, ERG11, ERG2 and TAC1) and gene expression by quantitative PCR (CDR1, CDR2 and ERG11)...
September 2015: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/25919124/multilocus-sequence-typing-of-pathogenic-candida-albicans-isolates-collected-from-a-teaching-hospital-in-shanghai-china-a-molecular-epidemiology-study
#14
Kefei Wu, Tao Luo, Li Li, Qiangqiang Zhang, Junhao Zhu, Qian Gao, Min Chen, Min Zhu
Molecular typing of Candida albicans is important for studying the population structure and epidemiology of this opportunistic yeast, such as population dynamics, nosocomial infections, multiple infections and microevolution. The genetic diversity of C. albicans has been rarely studied in China. In the present study, multilocus sequence typing (MLST) was used to characterize the genetic diversity and population structure of 62 C. albicans isolates collected from 40 patients from Huashan Hospital in Shanghai, China...
2015: PloS One
https://www.readbyqxmd.com/read/25720562/clinical-and-microbiological-investigation-of-fungemia-from-four-hospitals-in-china
#15
Danfeng Dong, Zhen Li, Lihua Zhang, Cen Jiang, Enqiang Mao, Xuefeng Wang, Yibing Peng
In this study, fungemia cases from four tertiary hospitals located in Shanghai and Anhui province in China from March 2012 to December 2013 were enrolled to investigate clinical features, species distribution, antifungal susceptibility and strain relatedness. During the study period, 137 non-duplicate cases and their corresponding isolates were collected. Six different genera of fungi were identified, of which Candida spp. were the most common (126/137, 91.97 %), with C. albicans predominating (48/137, 35.03 %)...
June 2015: Mycopathologia
https://www.readbyqxmd.com/read/25596113/multilocus-sequence-typing-of-candida-albicans-isolates-from-a-burn-intensive-care-unit-in-iran
#16
Mohammad H Afsarian, Hamid Badali, Teun Boekhout, Tahereh Shokohi, Farzad Katiraee
Burn intensive care unit (BICU) patients are specifically exposed to deep-seated nosocomial infections due to Candida albicans. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. Multilocus sequence typing is a powerful genotyping method for pathogenic micro-organisms, including Candida albicans. Thirty clinical isolates of C. albicans obtained from 22 patients that were admitted to the BICU from a burn hospital at Sari, Mazandaran state, Iran, were studied epidemiologically by multilocus sequence typing (MLST)...
March 2015: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/25533826/multilocus-sequence-typing-indicates-diverse-origins-of-invasive-candida-tropicalis-isolates-in-china
#17
Xin Fan, Meng Xiao, He Wang, Li Zhang, Fanrong Kong, Juan Lu, Zhidong Hu, Mei Kang, Yingchun Xu
BACKGROUND: According to data from the China Hospital Invasive Fungal Surveillance Net (CHIF-NET) 2010, Candida tropicalis (C. tropicalis) is the third most common pathogen causing invasive candidiasis. Moreover, the majority of fluconazole-resistant C. tropicalis isolates were from a single hospital. Therefore, a molecular epidemiological survey is necessary to investigate the genetic relatedness of C. tropicalis isolates in China. METHODS: In this study, 48 C...
2014: Chinese Medical Journal
https://www.readbyqxmd.com/read/25352939/fluconazole-resistance-patterns-in-candida-species-that-colonize-women-with-hiv-infection
#18
Lulu Zhang, Xiaodong She, Daniel Merenstein, Cuiwei Wang, Pilar Hamilton, Amanda Blackmon, Haihong Hu, Richard Calderone, Dongmei Li
BACKGROUND: The Women's Interagency HIV Study was established in 1993 to study the natural history of HIV disease among women in the United States. It currently has enrolled 2,895 women testing positive for HIV infection and 972 women without HIV infection recruited from 6 national metropolitan locations. The clinical database information collected for each HIV-positive individual included CD4 cell counts, viral load, and antiviral treatment to evaluate HIV prognosis and related conditions in women...
December 2014: Current Therapeutic Research, Clinical and Experimental
https://www.readbyqxmd.com/read/25351710/genetic-and-phenotypic-characterization-of-candida-albicans-strains-isolated-from-infectious-disease-patients-in-shanghai
#19
Lvyin Hu, Xin Du, Tianming Li, Yan Song, Shubei Zai, Xiangnan Hu, Xiaonan Zhang, Min Li
Candida albicans, as an opportunistic pathogen, can cause superficial and life-threatening candidiasis in immunocompromised individuals. The formation of surface-associated biofilms and the appearance of drug resistance pose a significant challenge for clinical intervention. In this study, a total of 104 hospital-acquired C. alibcans clinical isolates were collected from sterile sites and mucosal lesions of 92 infectious disease patients in the Shanghai Public Health Clinical Center and analysed. The resistance rates to fluconazole, itraconazole and voriconazole were 12...
January 2015: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/25054834/how-often-do-they-have-sex-a-comparative-analysis-of-the-population-structure-of-seven-eukaryotic-microbial-pathogens
#20
COMPARATIVE STUDY
Nicolás Tomasini, Juan José Lauthier, Francisco José Ayala, Michel Tibayrenc, Patricio Diosque
The model of predominant clonal evolution (PCE) proposed for micropathogens does not state that genetic exchange is totally absent, but rather, that it is too rare to break the prevalent PCE pattern. However, the actual impact of this "residual" genetic exchange should be evaluated. Multilocus Sequence Typing (MLST) is an excellent tool to explore the problem. Here, we compared online available MLST datasets for seven eukaryotic microbial pathogens: Trypanosoma cruzi, the Fusarium solani complex, Aspergillus fumigatus, Blastocystis subtype 3, the Leishmania donovani complex, Candida albicans and Candida glabrata...
2014: PloS One
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