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https://www.readbyqxmd.com/read/29581110/profiling-of-pdr1-and-msh2-in-candida-glabrata-bloodstream-isolates-from-a-multi-center-study-in-china
#1
Xin Hou, Meng Xiao, He Wang, Shu-Ying Yu, Ge Zhang, Yanan Zhao, Ying-Chun Xu
Among 158 Candida glabrata bloodstream isolates collected from multicenter in China, resistance to fluconazole were seen in 8.9% of isolates. Three isolates (1.9%) were resistant to all echinocandins. MLST ST7 (65.8%) was the most common type and followed by ST3 (7.6%). PDR1 polymorphisms were associated with acquisition of fluconazole resistance in C. glabrata isolates, while MSH2 polymorphisms were associated with the STs and microsatellite genotypes, irrespective of fluconazole resistance.
March 26, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29454824/candida-albicans-biology-molecular-characterization-pathogenicity-and-advances-in-diagnosis-and-control-an-update
#2
REVIEW
Maryam Dadar, Ruchi Tiwari, Kumaragurubaran Karthik, Sandip Chakraborty, Youcef Shahali, Kuldeep Dhama
Candida albicans is an emerging multidrug-resistant fungal pathogen representing an important source of invasive disease in humans and generating high healthcare costs worldwide. This fungus is frequently found in different anatomical sites of healthy persons and could induce systemic and superficial infections under optimal environmental conditions. Invasive candidiasis (IC) is an important nosocomial infection with high morbidity and mortality rates in hospitalized children. It represents a major source of prolonged infections in intensive care unit (ICU), particularly in immunosuppressed or elderly patients...
April 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29339049/genotype-and-homology-analysis-of-pathogenic-and-colonization-strains-of-candida-albicans-from-hospitalized-neonates
#3
Jin-Zhen Su, Yu-Lan Yang, Rong Rong, Ben-Qing Wu
BACKGROUND: To detect the genotypes of pathogenic and colonization Candida albicans strains and to reveal whether there was a homologous relationship between these strains. METHODS: Pathogenic and colonization isolates were collected from infants in the NICU of Shenzhen People's Hospital (Shenzhen, People's Republic of China). rDNA identification, multilocus sequence typing (MLST), and multi-loci variable number tandem repeat analysis (MLVA) were used for species confirmation, strain identification, phylogenetic tree clustering, and assessment of homology among the pathogenic and colonization strains...
December 26, 2017: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/28972465/prevalence-and-genetic-diversity-of-trichomonas-vaginalis-in-the-general-population-of-granada-and-co-infections-with-gardnerella-vaginalis-and-candida-species
#4
José Antonio Carrillo-Ávila, María Luisa Serrano-García, Jorge Fernández-Parra, Antonio Sorlózano-Puerto, José María Navarro-Marí, C Rune Stensvold, Jose Gutiérrez-Fernández
PURPOSE: Purulent or exudative genitourinary infections are a frequent cause of consultation in primary and specialized healthcare. The objectives of this study were: to determine the prevalence of Trichomonas vaginalis and co-infections with Candida spp. and Gardnerella vaginalis in vaginal secretion; and to use multilocus sequence typing (MLST) to analyse the genetic diversity of T. vaginalis strains. METHODOLOGY: The samples were submitted for analysis (n=5230) to a third-level hospital in Granada (Southern Spain) between 2011 and 2014; eight T...
October 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28854190/population-structure-and-molecular-genetic-characterization-of-clinical-candida-tropicalis-isolates-from-a-tertiary-care-hospital-in-kuwait-reveal-infections-with-unique-strains
#5
Khaled Al-Obaid, Mohammad Asadzadeh, Suhail Ahmad, Ziauddin Khan
Candida tropicalis is a frequently isolated yeast species causing bloodstream, urinary tract and other infections particularly in patients admitted to intensive care units (ICUs) and those requiring prolonged urinary catheterization (UC) or receiving broad-spectrum antibiotics (BSA). This study investigated clinical characteristics and genetic relatedness among C. tropicalis strains isolated from patients at Al-Amiri Hospital in Kuwait. C. tropicalis strains (n = 63) isolated from blood, genito-urinary, respiratory (RT) and digestive (GIT) tracts and wound sites from 54 patients were used...
2017: PloS One
https://www.readbyqxmd.com/read/28588560/molecular-epidemiology-and-antifungal-susceptibility-of-candida-glabrata-in-china-august-2009-to-july-2014-a-multi-center-study
#6
Xin Hou, Meng Xiao, Sharon C-A Chen, Fanrong Kong, He Wang, Yun-Zhuo Chu, Mei Kang, Zi-Yong Sun, Zhi-Dong Hu, Ruo-Yu Li, Juan Lu, Kang Liao, Tie-Shi Hu, Yu-Xing Ni, Gui-Ling Zou, Ge Zhang, Xin Fan, Yu-Pei Zhao, Ying-Chun Xu
Candida glabrata is an increasingly important cause of invasive candidiasis. In China, relatively little is known of the molecular epidemiology of C. glabrata and of its antifungal susceptibility patterns. Here we studied 411 non-duplicate C. glabrata isolates from 411 patients at 11 hospitals participating in the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET; 2010-2014). Genotyping was performed using multilocus sequence typing (MLST) employing six genetic loci and by microsatellite analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28587312/molecular-typing-of-staphylococcus-aureus-isolated-from-patients-with-autosomal-dominant-hyper-ige-syndrome
#7
Inka Sastalla, Kelli W Williams, Erik D Anderson, Ian A Myles, Jensen D Reckhow, Marlene Espinoza-Moraga, Alexandra F Freeman, Sandip K Datta
Autosomal dominant hyper IgE syndrome (AD-HIES) is a primary immunodeficiency caused by a loss-of-function mutation in the Signal Transducer and Activator of Transcription 3 (STAT3). This immune disorder is clinically characterized by increased susceptibility to cutaneous and sinopulmonary infections, in particular with Candida and Staphylococcus aureus. It has recently been recognized that the skin microbiome of patients with AD-HIES is altered with an overrepresentation of certain Gram-negative bacteria and Gram-positive staphylococci...
June 6, 2017: Pathogens
https://www.readbyqxmd.com/read/28490578/investigating-clinical-issues-by-genotyping-of-medically-important-fungi-why-and-how
#8
REVIEW
Alexandre Alanio, Marie Desnos-Ollivier, Dea Garcia-Hermoso, Stéphane Bretagne
Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes...
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28482076/genotyping-of-clinical-isolates-of-candida-glabrata-from-iran-by-multilocus-sequence-typing-and-determination-of-population-structure-and-drug-resistance-profile
#9
Saeid Amanloo, Masoomeh Shams-Ghahfarokhi, Mohammad Ghahri, Mehdi Razzaghi-Abyaneh
Candida glabrata is often the second most common causative agent for candidiasis following Candida albicans. Despite the importance of C. glabrata infections, few epidemiological studies have been conducted on this issue. The goal of this study was genotyping of clinical isolates of C. glabrata by multilocus sequence typing (MLST) technique for determination of the endemic prevalent genotypes and any association between isolation source and drug resistance. A total of 50 C. glabrata clinical isolates from Iran were analyzed by MLST and tested for in-vitro susceptibilities to amphotericin-B, caspofungin, fluconazole, and voriconazole according to the Clinical Laboratory Standards Institute (CLSI) M27-A4 document guidelines...
February 1, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28380072/population-structure-and-molecular-genetic-characterization-of-5-flucytosine-susceptible-and-resistant-clinical-candida-dubliniensis-isolates-from-kuwait
#10
Mohammad Asadzadeh, Suhail Ahmad, Noura Al-Sweih, Ziauddin Khan
Candida dubliniensis and Candida albicans are two closely related species. Although C. dubliniensis is less pathogenic, it has a higher propensity to develop resistance to fluconazole and some strains exhibit intrinsic resistance to 5-flucytosine (5-FC). All 5-FC-resistant isolates from Kuwait were previously shown to belong to one of seven internal transcribed spacer (ITS) region of rDNA-based haplotypes. This study performed fingerprinting of C. dubliniensis isolates by multilocus sequence typing (MLST) to determine population structure of 5-FC-resistant and -susceptible strains and compared the results with data from a global collection of isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28270801/molecular-fingerprinting-studies-do-not-support-intrahospital-transmission-of-candida-albicans-among-candidemia-patients-in-kuwait
#11
Mohammad Asadzadeh, Suhail Ahmad, Noura Al-Sweih, Ziauddin Khan
Candida albicans, a constituent of normal microbial flora of human mucosal surfaces, is a major cause of candidemia in immunocompromised individuals and hospitalized patients with other debilitating diseases. Molecular fingerprinting studies have suggested nosocomial transmission of C. albicans based on the presence of clusters or endemic genotypes in some hospitals. However, intrahospital strain transmission or a common source of infection has not been firmly established. We performed multilocus sequence typing (MLST) on 102 C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27915298/multilocus-sequence-typing-mlst-analysis-of-candida-albicans-isolates-colonizing-acrylic-dentures-before-and-after-denture-replacement
#12
Khai How Choo, Hee Ji Lee, Nicholas J Knight, Ann R Holmes, Richard D Cannon
Yeast, in particular Candida albicans, are the principal fungal cause of denture stomatitis, and can also be present as a commensal in many individuals. Few studies, however, have examined oral retention of yeast strains over time. We analyzed the yeast present in saliva samples and from the dentures of 10 individuals colonized with yeast but with no signs of stomatitis, before new complete maxillary dentures were fitted and also at 1, 3, and 6 months after denture replacement. Yeast species were presumptively identified on selective agar plates and were present in nine individuals before denture replacement and in six at the 6-month time point...
August 1, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27905002/disseminated-candidiasis-in-a-young-previously-healthy-dog-and-review-of-literature
#13
REVIEW
Nicole Willems, Dirk J Houwers, Yvette M Schlotter, Bart Theelen, Teun Boekhout
BACKGROUND: The reports on disseminated candidiasis in dogs so far describe at least one predisposing factor. This case report, however, highlights candidiasis in a dog without any known predisposition. PATIENT: A 1.5-year-old intact female Hovawart dog was presented with subcutaneous nodules and polyuria/polydipsia. An excisional biopsy revealed a chronic pyogranulomatous and necrotizing inflammation with mycotic structures. The patient became febrile and lethargic, and developed lameness...
June 2017: Mycopathologia
https://www.readbyqxmd.com/read/27855078/echinocandin-resistance-in-candida-species-isolates-from-liver-transplant-recipients
#14
Gwénolé Prigent, Nawel Aït-Ammar, Eric Levesque, Arnaud Fekkar, Jean-Marc Costa, Sarra El Anbassi, Françoise Foulet, Christophe Duvoux, Jean-Claude Merle, Eric Dannaoui, Françoise Botterel
Liver transplant recipients are at risk of invasive fungal infections, especially candidiasis. Echinocandin is recommended as prophylactic treatment but is increasingly associated with resistance. Our aim was to assess echinocandin drug resistance in Candida spp. isolated from liver transplant recipients treated with this antifungal class. For this, all liver-transplanted patients in a University Hospital (Créteil, France) between January and June of 2013 and 2015 were included. Susceptibilities of Candida isolates to echinocandins were tested by Etest and the EUCAST reference method...
February 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27456280/multilocus-sequence-typing-of-candida-tropicalis-shows-clonal-cluster-enrichment-in-azole-resistant-isolates-from-patients-in-shanghai-china
#15
Ying Wang, Ce Shi, Jin-Yan Liu, Wen-Jing Li, Yue Zhao, Ming-Jie Xiang
To explore the putative correlation between the multilocus sequence types (MLST) and antifungal susceptibility of clinical Candida tropicalis isolates in Mainland China. Eighty-two clinical C. tropicalis isolates were collected from sixty-nine patients at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China, from July 2012 to February 2015, and antifungal susceptibility tests were performed. Genetic profiles of those 82 isolates (30 azole-resistant and 52 azole-susceptible) were characterised by multilocus sequence typing...
October 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/26849050/clonal-strain-persistence-of-candida-albicans-isolates-from-chronic-mucocutaneous-candidiasis-patients
#16
Alexander J Moorhouse, Claire Rennison, Muhammad Raza, Desa Lilic, Neil A R Gow
Chronic mucocutaneous candidiasis (CMC) is a primary immunodeficiency disorder characterised by susceptibility to chronic Candida and fungal dermatophyte infections of the skin, nails and mucous membranes. Molecular epidemiology studies of CMC infection are limited in number and scope and it is not clear whether single or multiple strains inducing CMC persist stably or are exchanged and replaced. We subjected 42 C. albicans individual single colony isolates from 6 unrelated CMC patients to multilocus sequence typing (MLST)...
2016: PloS One
https://www.readbyqxmd.com/read/26848577/comparison-of-e-e-farnesol-secretion-and-the-clinical-characteristics-of-candida-albicans-bloodstream-isolates-from-different-multilocus-sequence-typing-clades
#17
Sook-In Jung, Jong Hee Shin, Soo Hyun Kim, Jin Kim, Joo Hee Kim, Min Ji Choi, Eun-Kyung Chung, Kyungwon Lee, Sun Hoe Koo, Hyun Ha Chang, Marie-Elisabeth Bougnoux, Christophe d'Enfert
Using multilocus sequence typing (MLST), Candida albicans can be subdivided into 18 different clades. Farnesol, a quorum-sensing molecule secreted by C. albicans, is thought to play an important role in the development of C. albicans biofilms and is also a virulence factor. This study evaluated whether C. albicans bloodstream infection (BSI) strains belonging to different MLST clades secrete different levels of E,E-farnesol (FOH) and whether they have different clinical characteristics. In total, 149 C. albicans BSI isolates from ten Korean hospitals belonging to clades 18 (n = 28), 4 (n = 23), 1 (n = 22), 12 (n = 17), and other clades (n = 59) were assessed...
2016: PloS One
https://www.readbyqxmd.com/read/26711776/posttreatment-antifungal-resistance-among-colonizing-candida-isolates-in-candidemia-patients-results-from-a-systematic-multicenter-study
#18
MULTICENTER STUDY
R H Jensen, H K Johansen, L M Søes, L E Lemming, F S Rosenvinge, L Nielsen, B Olesen, L Kristensen, E Dzajic, K M T Astvad, M C Arendrup
The prevalence of intrinsic and acquired resistance among colonizing Candida isolates from patients after candidemia was investigated systematically in a 1-year nationwide study. Patients were treated at the discretion of the treating physician. Oral swabs were obtained after treatment. Species distributions and MIC data were investigated for blood and posttreatment oral isolates from patients exposed to either azoles or echinocandins for <7 or ≥ 7 days. Species identification was confirmed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and internal transcribed spacer (ITS) sequencing, susceptibility was examined by EUCAST EDef 7...
December 28, 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26587501/molecular-diversity-of-candida-albicans-isolated-from-immunocompromised-patients-based-on-mlst-method
#19
Seyed Mohammad Hosein Afsarian, Hamid Badali, Tahereh Shokohi, Sohrab Najafipour
BACKGROUND: As regards multilocus sequence typing (MLST) method directly analyze the polymorphism within DNA sequences; we performed the first nationwide study on the genotypic relationships of Candida albicans strains obtained from oropharynx and bronchoalveolar lavage (BAL) samples from immunocompromised patients. METHODS: Fourteen epidemiologically unrelated clinical strains of C. albicans were obtained from three hospitals in Mazandaran Province, Iran (2006 to 2012) from seven patients with pulmonary infections and the rest with oropharyngeal samples of immunocompromised patients...
September 2015: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/26548511/evidence-of-genotypic-diversity-among-candida-auris-isolates-by-multilocus-sequence-typing-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-and-amplified-fragment-length-polymorphism
#20
A Prakash, C Sharma, A Singh, P Kumar Singh, A Kumar, F Hagen, N P Govender, A L Colombo, J F Meis, A Chowdhary
Candida auris is a multidrug-resistant nosocomial bloodstream pathogen that has been reported from Asian countries and South Africa. Herein, we studied the population structure and genetic relatedness among 104 global C. auris isolates from India, South Africa and Brazil using multilocus sequence typing (MLST), amplified fragment length polymorphism (AFLP) fingerprinting and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). RPB1, RPB2 and internal transcribed spacer (ITS) and D1/D2 regions of the ribosomal DNA were sequenced for MLST...
March 2016: Clinical Microbiology and Infection
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