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https://www.readbyqxmd.com/read/29040813/developmental-neurotoxic-effects-of-a-low-dose-of-tce-on-3d-neurosphere-system
#1
Mohamed Elsaid Abdraboh, Sherif H Abdeen, Mohamed Salama, Mahmoud Elhussiny, Yasser M El-Sherbini, Nada M Eldeen
Trichloroethylene (TCE) is one of the industrial toxic products that ever-present in air, soil and water. Several studies illustrated the toxicity of high doses of TCE on several organs. This study aims to determine the toxic effect of a low dose of TCE on development of rat neural stem cells (NSCs). Rat pups brains were collected, minced and harvested cells were cultured in the presence of neural growth factors B27/N8 to develop neurospheres. Afterwards, cells were subjected to a dose of 1µM TCE for 1 and 2 weeks...
October 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29035379/lobular-carcinoma-in-situ-and-invasive-lobular-breast-cancer-are-characterized-by-enhanced-expression-of-transcription-factor-ap-2%C3%AE
#2
Mieke Raap, Malte Gronewold, Henriette Christgen, Silke Glage, Mohammad Bentires-Alj, Shany Koren, Patrick W Derksen, Mirjam Boelens, Jos Jonkers, Ulrich Lehmann, Friedrich Feuerhake, Elna Kuehnle, Oleg Gluz, Ronald Kates, Ulrike Nitz, Nadia Harbeck, Hans H Kreipe, Matthias Christgen
Transcription factor AP-2β (TFAP2B) regulates embryonic organ development and is overexpressed in alveolar rhabdomyosarcoma, a rare childhood malignancy. Gene expression profiling has implicated AP-2β in breast cancer (BC). This study characterizes AP-2β expression in the mammary gland and in BC. AP-2β protein expression was assessed in the normal mammary gland epithelium, in various reactive, metaplastic and pre-invasive neoplastic lesions and in two clinical BC cohorts comprising >2000 patients. BCs from various genetically engineered mouse (GEM) models were also evaluated...
October 16, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29030822/analysis-of-murine-hematopoietic-stem-cell-proliferation-during-inflammation
#3
Emilie Jalbert, Eric M Pietras
Normally, quiescent hematopoietic stem cells (HSC) rapidly enter the cell cycle following exposure to inflammatory stimuli. The analysis of HSC cell cycle activity in murine bone marrow during inflammation is often complicated by the relative rarity of HSCs and shifts in Sca-1, a key cell surface marker used to identify HSCs. Here, we report a method to analyze HSC proliferation and cell cycle distribution under inflammatory conditions. Our approach uses EdU incorporation and Ki67 staining coupled with DNA content quantification by DAPI...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29029412/the-value-of-phosphohistone-h3-as-a-proliferation-marker-for-evaluating-invasive-breast-cancers-a-comparative-study-with-ki67
#4
Ji-Ye Kim, Hyang Sook Jeong, Taek Chung, Moonsik Kim, Ji Hee Lee, Woo Hee Jung, Ja Seung Koo
BACKGROUND: Established measurements of proliferation in breast cancer are Ki67 and mitotic-activity-index (MAI), with problems in reproducibility and prognostic accuracy. Phosphohistone H3 (PHH3), a relatively novel IHC marker is specific for mitosis with good reproducibility. We hypothesized that PHH3 would be more reproducible and better represent proliferation than Ki67. RESULTS: PHH3 identified easily-missed mitosis by MAI, as demonstrated by upgrading M grade at diagnosis (n = 29/218, evenly distributed)...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29027031/low-level-laser-irradiation-induces-in-vitro-proliferation-of-stem-cells-from-human-exfoliated-deciduous-teeth
#5
Fernanda Ginani, Diego Moura Soares, Hugo Alexandre de Oliveira Rocha, Lélia Batista de Souza, Carlos Augusto Galvão Barboza
The aim of this study was to evaluate the effect of low-level laser irradiation (LLLI) on the proliferation and viability of stem cells from human exfoliated deciduous teeth (SHED). Cells were irradiated or not (control) with an InGaAlP laser diode (660 nm, 30 mW, continuous action mode) using two different energy densities (0.5 J/cm(2)-16 s; 1.0 J/cm(2)-33 s). Irradiation was performed at 0 and 48 h, with the laser probe fixed at a distance of 0.5 cm from the cells. Cell proliferation was analyzed at 0, 24, 48, and 72 h by the Trypan blue exclusion method and MTT assay...
October 12, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29026176/primary-astrocytic-tumours-and-paired-recurrences-have-similar-biological-features-in-idh1-tp53-and-tertp-mutation-and-mgmt-atrx-loss
#6
Xia Li, Jie Wei, Yixiong Liu, Peifeng Li, Linni Fan, Yingmei Wang, Mingyang Li, Danhui Zhao, Zhou Yu, Jing Ye, Ying Guo, Qingguo Yan, Shuangping Guo, Zhe Wang
Astrocytic tumours are the most common type of primary malignant brain tumour. Most astrocytic tumours will recur at some point after surgery. Currently, the combination of radiotherapy and chemotherapy does not prevent the recurrence of astrocytic tumours. In this study, we investigated the consistency in isocitrate dehydrogenase 1 (IDH1), tumour protein p53 (TP53) and telomerase reverse transcriptase promoter (TERTp) mutations during astrocytic tumour recurrence. We also evaluated the protein loss of O-6-methylguanine-DNA methyltransferase (MGMT) and alpha-thalassemia/mental retardation, X-linked (ATRX) during disease recurrence...
October 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29022070/surgical-resection-for-pulmonary-carcinoid-long-term-results-of-multicentric-study-the-importance-of-pathological-n-status-more-than-we-thought
#7
Giacomo Cusumano, Ludovic Fournel, Salvatore Strano, Diane Damotte, Marie Christine Charpentier, Antonio Galia, Alberto Terminella, Maurizio Nicolosi, Jean Francois Regnard, Marco Alifano
BACKGROUND: Histological subdivision into typical (TC) and atypical (AC) is crucial for treatment and prognosis of lung carcinoids but can be also very challenging, even for experts. In this study, we aimed to strengthen or reduce the prognostic value of several pathological, clinical, or per-operative factors some of which are still controversial. METHODS: We retrospectively reviewed clinical records related to 195 patients affected by TC (159) or AC (36) surgically treated between 2000 and 2014, in three different centers...
October 11, 2017: Lung
https://www.readbyqxmd.com/read/29019871/despite-diagnostic-morphology-many-mixed-endometrial-carcinomas-show-unexpected-immunohistochemical-staining-patterns
#8
Cathleen E Matrai, Edyta C Pirog, Lora Hedrick Ellenson
Historically, endometrial carcinomas have been classified primarily according to their histology. However, the use of immunohistochemistry has become commonplace in their evaluation, particularly in diagnostically challenging cases. Our objective was to evaluate mixed endometrial carcinomas using a well-established panel of biomarkers to assess the consistency and utility of these stains in clinical diagnosis. Eighteen cases comprised of various combinations of classical serous (SC), endometrioid (EC), clear cell (CC) morphologies were identified and subjected to a panel of immunohistochemical markers including p53, p16, Ki67, estrogen receptor, progesterone receptor, and Napsin A...
October 10, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/29019543/predictive-factors-of-axillary-metastasis-in-patients-with-breast-cancer-and-positive-sentinel-lymph-node-biopsy
#9
Olívio Feitosa Costa, Rafael Bustamante Castro, Cibele Vasconcelos Oliveira, Thais Vieira Nogueira Feitosa, Josualdo Justino Alves, Francisco Pimentel Cavalcante, Marcos Venício Alves Lima
OBJECTIVE: to evaluate the risk factors for the presence of non-sentinel axillary metastatic disease in patients with breast cancer and positive sentinel node biopsy. Methods: retrospective cross-sectional study of women with breast cancer operated at the Cancer Institute of Ceará between 2002 and 2012 and submitted to sentinel lymph node biopsy. RESULTS: Among 946 breast cancer patients, 331 underwent sentinel lymph node biopsy, which was positive in 83. These patients underwent axillary lymphadenectomy and 39 (46%) had metastases in other axillary lymph nodes...
July 2017: Revista do Colégio Brasileiro de Cirurgiões
https://www.readbyqxmd.com/read/29016864/loss-of-host-derived-osteopontin-creates-a-glioblastoma-promoting-microenvironment
#10
Frank Szulzewsky, Nina Schwendinger, Dilansu Güneykaya, Patrick J Cimino, Dolores Hambardzumyan, Michael Synowitz, Eric C Holland, Helmut Kettenmann
Background: Microglia and periphery-derived monocytes infiltrate human and mouse glioblastoma and their density is positively correlated with malignancy. Using microarray and RNA sequencing we have previously shown that glioblastoma-associated microglia/monocytes (GAMs) express osteopontin/SPP1. Methods: We used qRT-PCR, immunofluorescence stainings, western-blot, and flow-cytometry to identify the various sources of osteopontin (OPN) expression in human and mouse glioblastoma...
August 26, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28991260/protein-and-chemotherapy-profiling-of-extracellular-vesicles-harvested-from-therapeutic-induced-senescent-triple-negative-breast-cancer-cells
#11
E L Kavanagh, S Lindsay, M Halasz, L C Gubbins, K Weiner-Gorzel, M H Z Guang, A McGoldrick, E Collins, M Henry, A Blanco-Fernández, P O' Gorman, P Fitzpatrick, M J Higgins, P Dowling, A McCann
Triple negative breast cancer (TNBC) is an aggressive subtype with relatively poor clinical outcomes and limited treatment options. Chemotherapy, while killing cancer cells, can result in the generation of highly chemoresistant therapeutic induced senescent (TIS) cells that potentially form stem cell niches resulting in metastases. Intriguingly, senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells. Our aim was to profile EVs harvested from TIS TNBC cells compared with control cells to identify a potential mechanism by which TIS TNBC cells maintain survival in the face of chemotherapy...
October 9, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28991124/angiogenesis-in-the-transplanted-donor-graft-after-living-donor-liver-transplantation
#12
Dong-Hwan Jung, Sung-Hwan Moon, Soon-Jung Park, Eun Jae Kim, In Ho Jang, Cheon-Soo Park, Ji Yoon Lee, Yong-Pil Cho, Sung-Gyu Lee
BACKGROUND: There is no direct evidence for the role of angiogenesis in liver regeneration in humans. This study aimed to determine whether angiogenesis is involved in the regeneration of transplanted donor grafts in human living-donor liver transplantation (LDLT) and to examine the impact of donor graft volume on angiogenesis. METHODS: Clinical data and liver tissue characteristics were analyzed in 4 patients who received adult-to-adult LDLT with dual left lobe grafts from 2 living donors...
October 5, 2017: Transplantation
https://www.readbyqxmd.com/read/28990117/expression-of-matrix-metalloproteinase-12-is-highly-specific-for-non-proliferating-invasive-trophoblasts-in-the-first-trimester-and-temporally-regulated-by-oxygen-dependent-mechanisms-including-hif-1a
#13
Ursula Hiden, Christian P Eyth, Alejandro Majali-Martinez, Gernot Desoye, Carmen Tam-Amersdorfer, Berthold Huppertz, Nassim Ghaffari Tabrizi-Wizsy
During first trimester pregnancy, trophoblast cells invade from the placenta into the maternal decidua where they anchor the placenta and remodel luminal structures like spiral arteries. This process depends on proteases secreted by invading trophoblasts, which degrade extracellular matrix (ECM). We here aimed to identify proteases particularly important for trophoblast invasion. We generated a list of proteases capable of degrading decidual ECM and trophoblast integrins using MEROPS database and compared expression of these proteases between primary trophoblasts isolated from first trimester placenta (FT, n = 3), representing an invasive phenotype, vs trophoblasts isolated from term pregnancy (TT, n = 3), representing a non-invasive trophoblast phenotype...
October 9, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28988162/organised-screening-for-cervical-cancer-in-france-a-cost-effectiveness-assessment
#14
Stéphanie Barré, Marc Massetti, Henri Leleu, Frédéric De Bels
OBJECTIVE: According to the third cancer plan, organised screening (OS) of cervical cancer (CC) among women aged 25-65 years should be implemented in France in the forthcoming years. The most efficient way to implement OS in the French healthcare system is yet to be determined. METHODS: A microsimulation model was developed adopting a collective 'all payers' perspective. A closed cohort of women eligible for CC screening and representative in terms of age and participation in individual screening (IndScr) by annual Papanicolaou (Pap) testing every 3 years was modelled on a lifetime horizon...
October 6, 2017: BMJ Open
https://www.readbyqxmd.com/read/28987630/mri-texture-analysis-reflects-histopathology-parameters-in-thyroid-cancer-a-first-preliminary-study
#15
Hans-Jonas Meyer, Stefan Schob, Anne Kathrin Höhn, Alexey Surov
OBJECT: Thyroid cancer represents the most frequent malignancy of the endocrine system with an increasing incidence worldwide. Novel imaging techniques are able to further characterize tumors and even predict histopathology features. Texture analysis is an emergent imaging technique to extract extensive data from an radiology images. The present study was therefore conducted to identify possible associations between texture analysis and histopathology parameters in thyroid cancer. METHODS: The radiological database was retrospectively reviewed for thyroid carcinoma...
October 5, 2017: Translational Oncology
https://www.readbyqxmd.com/read/28986224/autophagy-apoptosis-and-cell-proliferation-in-exstrophy-epispadias-complex
#16
Mahsa Shabaninia, Ali Tourchi, Heather Di Carlo, John Gearhart
OBJECTIVE: To investigate the state of autophagy and its interactions with apoptosis and cell proliferation in patients who underwent successful early closure or delayed closure of exstrophy. They compared those outcomes with cell culture samples from patients with VUR as control. METHODS: Primary cultures of bladder smooth muscle cells were established from patients with successful neonatal bladder closure (group 1, N=5), delayed closure due to small bladder template (group 2, N=5) and vesicoureteral reflux as control (group3, N=5)...
October 3, 2017: Urology
https://www.readbyqxmd.com/read/28984338/synchronous-cervical-minimal-deviation-adenocarcinoma-gastric-type-adenocarcinoma-and-lobular-endocervical-glandular-hyperplasia-along-with-stil-in-peutz-jeghers-syndrome-eliciting-oncogenesis-pathways
#17
Azfar Neyaz, Nuzhat Husain, Manish Deodhar, Rohini Khurana, Saumya Shukla, Aditi Arora
We describe an unusual case of a Peutz-Jeghers syndrome associated with a composite synchronous cervical neoplasia comprising precursor "lobular endocervical glandular hyperplasia (LEGH)", "minimal deviation adenocarcinoma (MDA)" and "gastric-type adenocarcinoma (GTA)" along with a serous tubal intraepithelial lesion (STIL) in the right fallopian tube. A 24-year-old woman presented with a white mucoid discharge and bleeding per vaginum for one year. Histopathological evaluation showed MDA & GTA in FIGO grade III with pelvic lymph node metastasis despite a deceptively bland tumour morphology and low Ki-67 index, indicating an aggressive tumour course and poor prognosis...
October 6, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28984116/cerebellar-liponeurocytoma-with-atypical-histological-features-a-rare-example-of-a-glioneuronal-tumor
#18
Blanka Hermann, Michal Woznica, Wojciech Kloc, Piotr Borkowski, Witold Libionka, Ewa Izycka-Swieszewska
We present a case of a rare neoplasm in a 77-year-old woman with previous oncological history, who developed a rapid onset of cerebellar symptoms. The neuroimaging detected a posterior fossa tumor suspected of meningioma which was completely resected soon after. Histologically the neoplasm had two components with different immunophenotype. One constituent was lobular, composed of monotonous mitotically active round cells with a predominant neuronal profile. The second, astrocytic component contained lipomatous cells intermixed with larger gemistocytic astrocytes...
2017: Folia Neuropathologica
https://www.readbyqxmd.com/read/28983587/bmi%C3%A2-1%C3%A2-targeting-suppresses-osteosarcoma-aggressiveness-through-the-nf%C3%A2-%C3%AE%C2%BAb-signaling-pathway
#19
Jiaguo Liu, Bin Luo, Meng Zhao
Bone cancer is one of the most lethal malignancies and the specific causes of tumor initiation are not well understood. B‑cell‑specific Moloney murine leukemia virus integration site 1 protein (Bmi‑1) has been reported to be associated with the initiation and progression of osteosarcoma, and as a prognostic indicator in the clinic. In the current study, a full‑length antibody targeting Bmi‑1 (AbBmi‑1) was produced and the preclinical value of Bmi‑1‑targeted therapy was evaluated in bone carcinoma cells and tumor xenograft mice...
September 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28982944/brown-adipocytes-postnatally-arise-through-both-differentiation-from-progenitors-and-conversion-from-white-adipocytes-in-syrian-hamster
#20
Yuko Okamatsu-Ogura, Junko Nio-Kobayashi, Kazuki Nagaya, Ayumi Tsubota, Kazuhiro Kimura
To investigate the postnatal development of brown adipose tissue (BAT) in Syrian hamsters, we histologically examined interscapular fat tissue from 5 to 16-day-old pups, focusing on how brown adipocytes arise. Interscapular fat of 5-day-old hamsters mainly consisted of white adipocytes containing large unilocular lipid droplets, as observed in typical white adipose tissue (WAT). On day 7, clusters of small, proliferative nonadipocytes with a strong immunoreactivity for Ki67 appeared near the edge of the interscapular fat tissue...
October 5, 2017: Journal of Applied Physiology
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