Nathanael A Caveney, Grayson E Rodriguez, Christoph Pollmann, Thomas Meyer, Marta T Borowska, Steven C Wilson, Nan Wang, Xinyu Xiang, Karsten D Householder, Pingdong Tao, Leon L Su, Robert A Saxton, Jacob Piehler, K Christopher Garcia
Cytokines regulate immune responses by binding to cell surface receptors, including the common subunit beta (βc), which mediates signaling for GM-CSF, IL-3, and IL-5. Despite known roles in inflammation, the structural basis of IL-5 receptor activation remains unclear. We present the cryo-EM structure of the human IL-5 ternary receptor complex, revealing architectural principles for IL-5, GM-CSF, and IL-3. In mammalian cell culture, single-molecule imaging confirms hexameric IL-5 complex formation on cell surfaces...
April 5, 2024: Molecular Cell