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Beta cell imaging

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https://www.readbyqxmd.com/read/28614933/-study-on-the-establishment-of-hmld-model-and-the-expression-of-kl-6-tgf-beta-in-rat
#1
Z S Zhang, Y F Zhao, L Tang, H K Xie, D Y Sun
Objective: To establish an animal model of hard metal lung disease (HMLD) in rats, and to screen the indications for diagnosis of HMLD. Methods: The rats were randomly divided into 5 groups, each group included 8 rats: saline group, pure cobalt group, pure tungsten carbide group, silica group and hard metal (HM) group. 10 mg subjects were administered in each group by using the pulmonary endotracheal tube. After 8 week, the lung CT scan and lung tissue pathology were observed, the serum and bronchoalveolar lavage fluid (BALF) were collected for KL-6, TGF-beta1 and TGF-beta2...
April 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28601914/left-ventricular-ejection-fraction-as-therapeutic-target-is-it-the-ideal-marker
#2
REVIEW
V Katsi, G Georgiopoulos, A Laina, E Koutli, J Parissis, C Tsioufis, P Nihoyannopoulos, D Tousoulis
Heart failure (HF) consists the fastest growing clinical cardiac disease. HF patients are categorized on the basis of underlying left ventricular ejection fraction (LVEF) into HF with preserved EF (HFpEF), reduced LVEF (HFrEF), and mid-range LVEF (HFmrEF). While LVEF is the most commonly used surrogate marker of left ventricular (LV) systolic function, the implementation of two-dimensional echocardiography in estimating this parameter imposes certain caveats on current HF classification. Most importantly, LVEF could fluctuate in repeated measurements or even recover after treatment, thus blunting the borders between proposed categories of HF and enabling upward classification of patients...
June 10, 2017: Heart Failure Reviews
https://www.readbyqxmd.com/read/28601278/laquinimod-has-no-effects-on-brain-volume-or-cellular-cns-composition-in-the-f1-3xtg-ad-c3h-mouse-model-of-alzheimer-s-disease
#3
Rehana Z Hussain, William A Miller-Little, Doris Lambracht-Washington, Tom C Jaramillo, Masaya Takahashi, Shanrong Zhang, Min Fu, Gary R Cutter, Liat Hayardeny, Craig M Powell, Roger N Rosenberg, Olaf Stüve
BACKGROUND: Laquinimod is an anti-inflammatory agent with good central nervous system (CNS) bioavailability, and neuroprotective and myelorestorative properties. A clinical trial in patients with multiple sclerosis demonstrated that laquinimod significantly reduced loss of brain volume. The cellular substrate or molecular events underlying that treatment effect are unknown. In this study, we aimed to explore laquinimod's potential effects on brain volume, animal behavior, cellular numbers and composition of CNS-intrinsic cells and mononuclear cells within the CNS, amyloid beta (Aβ) accumulation and tau phosphorylation in the F1 3xTg-AD/C3H mouse model of Alzheimer's disease...
August 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28599005/formation-and-spreading-of-tdp-43-aggregates-in-cultured-neuronal-and-glial-cells-demonstrated-by-time-lapse-imaging
#4
Tomohiro Ishii, Emiko Kawakami, Kentaro Endo, Hidemi Misawa, Kazuhiko Watabe
TAR DNA-binding protein 43 (TDP-43) is a main constituent of cytoplasmic aggregates in neuronal and glial cells in cases of amyotrophic lateral sclerosis and frontotemporal lobar degeneration. We have previously demonstrated that adenovirus-transduced artificial TDP-43 cytoplasmic aggregates formation is enhanced by proteasome inhibition in vitro and in vivo. However, the relationship between cytoplasmic aggregate formation and cell death remains unclear. In the present study, rat neural stem cell lines stably transfected with EGFP- or Sirius-expression vectors under the control of tubulin beta III, glial fibrillary acidic protein, or 2',3'-cyclic nucleotide 3'-phosphodiesterase promoter were differentiated into neurons, astrocytes, and oligodendrocytes, respectively, in the presence of retinoic acid...
2017: PloS One
https://www.readbyqxmd.com/read/28556971/low-level-laser-therapy-780%C3%A2-nm-combined-with-collagen-sponge-scaffold-promotes-repair-of-rat-cranial-critical-size-defects-and-increases-tgf-%C3%AE-fgf-2-opg-rank-and-osteocalcin-expression
#5
Lana Sarita de Souza de Oliveira, Aurigena Antunes de Araújo, Raimundo Fernandes de Araújo Júnior, Carlos Augusto Galvão Barboza, Boniek Castillo Dutra Borges, José Sandro Pereira da Silva
The aim of this study was to evaluate the effect of collagen sponge scaffold (CSS) implantation associated with low-level laser therapy (LLLT) on repairing bone defects. A single 5-mm cranial defect was surgically created in forty Wistar rats, which then received one of the following four interventions (n = 10 per group): no treatment (G0); bone defect implanted with collagen sponge scaffold (CSS) alone (G1); defect treated with low-level laser therapy (LLLT) (wavelength 780 nm; total energy density 120 J/cm(2) ; power 50 mW) alone (G2); and CSS associated with LLLT treatment (G3)...
May 29, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28511918/molecular-imaging-of-serotonin-degeneration-in-mild-cognitive-impairment
#6
Gwenn S Smith, Frederick S Barrett, Jin Hui Joo, Najlla Nassery, Alena Savonenko, Devin J Sodums, Christopher M Marano, Cynthia A Munro, Jason Brandt, Michael A Kraut, Yun Zhou, Dean F Wong, Clifford I Workman
Neuropathological and neuroimaging studies have consistently demonstrated degeneration of monoamine systems, especially the serotonin system, in normal aging and Alzheimer's disease. The evidence for degeneration of the serotonin system in mild cognitive impairment is limited. Thus, the goal of the present study was to measure the serotonin transporter in vivo in mild cognitive impairment and healthy controls. The serotonin transporter is a selective marker of serotonin terminals and of the integrity of serotonin projections to cortical, subcortical and limbic regions and is found in high concentrations in the serotonergic cell bodies of origin of these projections (raphe nuclei)...
May 13, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/28500860/interventions-for-preventing-silent-cerebral-infarcts-in-people-with-sickle-cell-disease
#7
REVIEW
Lise J Estcourt, Patricia M Fortin, Sally Hopewell, Marialena Trivella, Carolyn Doree, Miguel R Abboud
BACKGROUND: Sickle cell disease (SCD) is one of the commonest severe monogenic disorders in the world, due to the inheritance of two abnormal haemoglobin (beta globin) genes. SCD can cause severe pain, significant end-organ damage, pulmonary complications, and premature death. Silent cerebral infarcts are the commonest neurological complication in children and probably adults with SCD. Silent cerebral infarcts also affect academic performance, increase cognitive deficits and may lower intelligence quotient...
May 13, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28496188/preclinical-evaluation-of-pac1-targeting-with-radiolabeled-maxadilan
#8
Lieke Joosten, Maarten Brom, Martin K H Schäfer, Otto C Boerman, Eberhard Weihe, Martin Gotthardt
There is an ongoing search for new tracers to optimize imaging of beta cell-derived tumors (insulinomas). The PAC1 receptor, expressed by insulinomas, can be used for targeting of these tumors. Here, we investigated whether radiolabeled maxadilan could be used for insulinoma imaging. Maxadilan was C- or N-terminally conjugated with DTPA (termed maxadilan-DPTA or DTPA-maxadilan respectively). BALB/c nude mice bearing subcutaneous INS-1 tumors were injected with either In-111-labeled maxadilan-DTPA or In-111-DTPA-maxadilan...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486025/efficacy-and-persistence-of-allogeneic-adipose-derived-mesenchymal-stem-cells-combined-with-hyaluronic-acid-in-osteoarthritis-after-intra-articular-injection-in-a-sheep-model
#9
Chenchen Feng, Xuan Luo, Na He, Huitang Xia, Xiaoteng Lv, Xue Zhang, Dan Li, Fei Wang, Jiaping He, Li Zhang, Xiangming Lin, Liping Lin, Huabin Yin, Jiyin He, Jinwu Wang, Wei Cao, Richard Wang, Guangdong Zhou, Wen Wang
Although a number of studies have reported efficacy of autologous adipose-derived mesenchymal stem cells (AD-MSCs) in treating osteoarthritis (OA), no reliable evidences demonstrate whether allogeneic AD-MSCs can efficiently block OA progression in a large animal model. The current study explored the efficacy and survival of allogeneic AD-MSCs combined with hyaluronic acid (HA) after intra-articular (IA) injection in a sheep OA model, which were conventionally established by anterior cruciate ligament resection and medial meniscectomy...
May 9, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28467339/the-primitive-extratesticular-seminoma-diagnosis-of-a-rare-pathology
#10
Luca Saba
 Background: The Primitive Extratesticular Seminoma is a very rare condition and represents 3% of germ cell tumors; it is an indeterminate origin disease, whose diagnosis is often complicated by a nonspecific and highly variable clinical finding. CASE PRESENTATION: A caucasian 55 years old male, non-smoker, arrived to our centre with cough, severe respiratory distress and dysphagia, in a context of the superior vena cava syndrome. A Computed Tomography was performed, which shows the presence of a mediastinal anterior mass with aorto-pulmonary window and left paracardiac invasion...
April 28, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/28461162/bnn-20-a-synthetic-microneurotrophin-strongly-protects-dopaminergic-neurons-in-the-weaver-mouse-a-genetic-model-of-dopamine-denervation-acting-through-the-trkb-neurotrophin-receptor
#11
Konstantinos Botsakis, Theodora Mourtzi, Vasiliki Panagiotakopoulou, Malamati Vreka, Georgios T Stathopoulos, Iosif Pediaditakis, Ioannis Charalampopoulos, Achilleas Gravanis, Foteini Delis, Katerina Antoniou, Dimitrios Zisimopoulos, Christos D Georgiou, Nikolaos T Panagopoulos, Nikolaos Matsokis, Fevronia Angelatou
Neurotrophic factors are among the most promising treatments aiming at slowing or stopping and even reversing Parkinson's disease (PD). However, in most cases, they cannot readily cross the human blood-brain-barrier (BBB). Herein, we propose as a therapeutic for PD the small molecule 17-beta-spiro-[5-androsten-17,2'-oxiran]-3beta-ol (BNN-20), a synthetic analogue of DHEA, which crosses the BBB and is deprived of endocrine side-effects. Using the "weaver" mouse, a genetic model of PD, which exhibits progressive dopaminergic neurodegeneration in the Substantia Nigra (SN), we have shown that long-term administration (P1-P21) of BNN-20 almost fully protected the dopaminergic neurons and their terminals, via i) a strong anti-apoptotic effect, probably mediated through the Tropomyosin receptor kinase B (TrkB) neurotrophin receptor's PI3K-Akt-NF-κB signaling pathway, ii) by exerting an efficient antioxidant effect, iii) by inducing significant anti-inflammatory activity and iv) by restoring Brain-Derived Neurotrophic Factor (BDNF) levels...
April 28, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28455874/quantitative-identification-of-senescent-cells-in-aging-and-disease
#12
Anat Biran, Lior Zada, Paula Abou Karam, Ezra Vadai, Lior Roitman, Yossi Ovadya, Ziv Porat, Valery Krizhanovsky
Senescent cells are present in premalignant lesions and sites of tissue damage and accumulate in tissues with age. In vivo identification, quantification and characterization of senescent cells are challenging tasks that limit our understanding of the role of senescent cells in diseases and aging. Here, we present a new way to precisely quantify and identify senescent cells in tissues on a single-cell basis. The method combines a senescence-associated beta-galactosidase assay with staining of molecular markers for cellular senescence and of cellular identity...
April 28, 2017: Aging Cell
https://www.readbyqxmd.com/read/28441080/color-coded-intravital-imaging-demonstrates-a-transforming-growth-factor-%C3%AE-tgf-%C3%AE-antagonist-selectively-targets-stromal-cells-in-a-human-pancreatic-cancer-orthotopic-mouse-model
#13
Takashi Murakami, Yukihiko Hiroshima, Kentaro Miyake, Ho Kyoung Hwang, Tasuku Kiyuna, Jonathan C DeLong, Thinzar M Lwin, Ryusei Matsuyama, Ryutaro Mori, Takafumi Kumamoto, Takashi Chishima, Kuniya Tanaka, Yasushi Ichikawa, Michael Bouvet, Itaru Endo, Robert M Hoffman
Pancreatic cancer is a recalcitrant malignancy, partly due to desmoplastic stroma which stimulates tumor growth, invasion, and metastasis, and inhibits chemotherapeutic drug delivery. Transforming growth factor-β (TGF-β) has an important role in the formation of stromal desmoplasia. The present study describes the ability of color-coded intravital imaging to demonstrate the efficacy of a TGF-β inhibitor to target stroma in an orthotopic mouse model of pancreatic cancer. The BxPC-3 human pancreatic adenocarcinoma cell line expressing green fluorescent protein (GFP), which also has a high TGF-β expression level, was used in an orthotopic model in transgenic nude mice ubiquitously expressing red fluorescent protein (RFP)...
May 19, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28431540/prevention-of-advanced-glycation-end-products-formation-in-diabetic-rats-through-beta-cell-modulation-by-aegle-marmelos
#14
Rahman M Hafizur, Shahrukh Momin, Noor Fatima
BACKGROUND: Although the anti-diabetic activity of Aegle marmelos (AM) is known, however, its anti-glycation activity is not reported yet. In this study, we have investigated its anti-glycation activity under in vitro and in vivo conditions and determined possible mechanism(s) in streptozotocin-induced diabetic rats. METHODS: Effective dose of AM (400 mg/kg) was administrated orally to diabetic rats for 42 days. Thereafter, blood glucose, serum insulin, HbA1c, antioxidant status, and advanced glycation end-products (AGEs) were measured...
April 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28423568/sepia-ink-oligopeptide-induces-apoptosis-and-growth-inhibition-in-human-lung-cancer-cells
#15
Zhi Zhang, Lei Sun, Guoren Zhou, Peng Xie, Jinjun Ye
Sepia ink oligopeptide (SIO), as a tripeptide extracted from Sepia ink, could be used as an inducer of apoptosis in human prostate cancer cells. We designed a cyclo-mimetic peptide of SIO by introducing a disulfide bond to stabilize the native peptide into beta turn structure, and produced a peptide with higher cell permeability and stability. Through labeling an FITC to the N-terminus of the peptide, the cell permeability was examined. Stabilized peptide showed enhanced cellular uptake than linear tripeptide as indicated by flow cytometry and cell fluorescent imaging...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28409274/toward-molecular-imaging-of-the-free-fatty-acid-receptor-1
#16
Ewa Hellström-Lindahl, Ola Åberg, Cecilia Ericsson, Gavin O'Mahony, Peter Johnström, Stanko Skrtic, Olof Eriksson
AIMS: Molecular imaging of the free fatty acid receptor 1 (FFAR1) would be a valuable tool for drug development by enabling in vivo target engagement studies in human. It has also been suggested as a putative target for beta cell imaging, but the inherent lipophilicity of most FFAR1 binders produces high off-target binding, which has hampered progress in this area. The aim of this study was to generate a suitable lead compound for further PET labeling. METHODS: In order to identify a lead compound for future PET labeling for quantitative imaging of FFAR1 in human, we evaluated tritiated small molecule FFAR1 binding probes ([(3)H]AZ1, [(3)H]AZ2 and [(3)H]TAK-875) for their off-target binding, receptor density and affinity in human pancreatic tissue (islets and exocrine) and rodent insulinoma...
July 2017: Acta Diabetologica
https://www.readbyqxmd.com/read/28401888/advances-in-pancreatic-islet-monolayer-culture-on-glass-surfaces-enable-super-resolution-microscopy-and-insights-into-beta-cell-ciliogenesis-and-proliferation
#17
Edward A Phelps, Chiara Cianciaruso, Jaime Santo-Domingo, Miriella Pasquier, Gabriele Galliverti, Lorenzo Piemonti, Ekaterine Berishvili, Olivier Burri, Andreas Wiederkehr, Jeffrey A Hubbell, Steinunn Baekkeskov
A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and live-cell microscopy. Guided by an observation that dispersed islet cells spread and adhere well on glass surfaces in neuronal co-culture and form a monolayer of connected cells, we demonstrate that in the absence of neurons, well-defined surface coatings combined with components of neuronal culture media collectively support robust attachment and growth of primary human or rat islet cells as monolayers on glass surfaces...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28397263/protective-effect-of-cirsimaritin-against-streptozotocin-induced-apoptosis-in-pancreatic-beta-cells
#18
Dahae Lee, Ki Hyun Kim, Jaemin Lee, Gwi Seo Hwang, Hye Lim Lee, Dae-Hyun Hahm, Chang Ki Huh, Sang Cheon Lee, Sanghyun Lee, Ki Sung Kang
OBJECTIVES: Maintaining glucose homoeostasis is essential for the survival of cells. Despite the various health benefits of Korean thistle (Cirsium japonicum var. maackii), their effects on pancreatic β-cell apoptosis in type 1 diabetes mellitus and the underlying mechanisms remain unclear, and experimentally investigated in this study. METHODS: The effects of C. japonicum var. maackii and its active component cirsimaritin against streptozotocin (STZ)-induced cytotoxicity were assessed in INS-1 cells...
April 11, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28390866/activation-of-transforming-growth-factor-beta-1-signaling-in-gastric-cancer-associated-fibroblasts-increases-their-motility-via-expression-of-rhomboid-5-homolog-2-and-ability-to-induce-invasiveness-of-gastric-cancer-cells
#19
Takatsugu Ishimoto, Keisuke Miyake, Tannistha Nandi, Masakazu Yashiro, Nobuyuki Onishi, Kie Kyon Huang, Lin Suling Joyce, Ramnarayanan Kalpana, Su Ting Tay, Yuka Suzuki, Byoung Chul Cho, Daisuke Kuroda, Kota Arima, Daisuke Izumi, Masaaki Iwatsuki, Yoshifumi Baba, Eiji Oki, Masayuki Watanabe, Hideyuki Saya, Kosei Hirakawa, Hideo Baba, Patrick Tan
BACKGROUND & AIMS: Fibroblasts that interact with cancer cells are called cancer-associated fibroblasts (CAFs), which promote progression of different tumor types. We investigated the characteristics and functions of CAFs in diffuse-type gastric cancers (DGCs) by analyzing features of their genome and gene expression patterns. METHODS: We isolated CAFs and adjacent non-cancer fibroblasts (NFs) from 110 gastric cancer (GC) tissues from patients who underwent gastrectomy in Japan from 2008 through 2016...
April 5, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28385164/diffuse-idiopathic-pulmonary-neuroendocrine-cell-hyperplasia-diagnosed-by-transbronchial-lung-cryobiopsy-a-case-report
#20
R Sauer, S Griff, A Blau, A Franke, T Mairinger, C Grah
BACKGROUND: Micronodular lesions are common findings in lung imaging. As an important differential diagnosis, we describe a case of diffuse idiopathic pulmonary neuroendocrine cell hyperplasia; it is notable that the diagnosis of diffuse idiopathic pulmonary neuroendocrine cell hyperplasia is often delayed. This case provides supporting evidence to establish lung biopsy by cryotechnique as the option of first choice when considering a diagnostic strategy for micronodular lung lesions...
April 7, 2017: Journal of Medical Case Reports
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