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https://www.readbyqxmd.com/read/28814955/characterization-of-carbapenem-resistant-acinetobacter-baumannii-strains-isolated-from-hospitalized-patients-in-palestine
#1
Regeen Handal, Lulu Qunibi, Ibrahim Sahouri, Maha Juhari, Rula Dawodi, Hiyam Marzouqa, Musa Hindiyeh
The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β-lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28811083/phenotypic-chlorhexidine-and-triclosan-susceptibility-in-clinical-staphylococcus-aureus-isolates-in-australia
#2
C Hughes, J Ferguson
Antiseptics such as chlorhexidine gluconate and triclosan are widely used in healthcare settings for both skin antisepsis and decolonisation of Staphylococcus aureus. We determined the minimum inhibitory concentration (MIC) of 198 methicillin susceptible and resistant Staphylococcus aureus clinical isolates to both chlorhexidine and triclosan using an agar dilution method. Of these, 10% (19/198) showed a raised MIC to chlorhexidine and 3% (6/198) showed an elevated MIC to triclosan. The multilocus sequence type (MLST) of each isolate was predicted using a binary method, and although ST93-MRSA-IV was the most common, ST22-MRSA-IV was shown to have statistically higher chlorhexidine MIC values compared with non ST22-MRSA-IV isolates (z = -8...
August 12, 2017: Pathology
https://www.readbyqxmd.com/read/28810248/antibiotic-susceptibility-and-molecular-characterization-of-escherichia-coli-o157-isolates-from-urinary-tract-infections
#3
Zonglan Xie, Ziqiang Dong, Ping Zhu, Lusheng Zhang, Xiaobo Chen, Chuanjiang Dong
BACKGROUND: Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. OBJECTIVES: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E...
August 16, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28809435/first-case-report-of-non-human-primates-alouatta-clamitans-with-the-hypervirulent-klebsiella-pneumoniae-serotype-k1-strain-st-23-a-possible-emerging-wildlife-pathogen
#4
Eleine Kuroki Anzai, Júlio César de Souza Júnior, Amanda Rezende Peruchi, Juliana Mello Fonseca, Elke Kreuscher Gumpl, Antônio Carlos Campos Pignatari, Zelinda Maria Braga Hirano, Alessandro Conrado de Oliveira Silveira
BACKGROUND: Hypervirulent strain of Klebsiella pneumoniae genotype K1 isolates have recently emerged, causing severe pyogenic liver abscess complicated by devastating metastatic infections in humans. METHODS: We describe a short outbreak of the non-human primate (NHP) research center, associated with a hypervirulent K. pneumoniae. The genetic similarity of the strains was evaluated by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques, and virulence encoding genes were detected by polymerase chain reaction (PCR)...
August 15, 2017: Journal of Medical Primatology
https://www.readbyqxmd.com/read/28807097/genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-pigs-and-retail-foods-in-china
#5
Wei Wang, Feng Liu, Zulqarnain Baloch, Cun Shan Zhang, Ke Ma, Zi Xin Peng, Shao Fei Yan, Yu Jie Hu, Xin Gan, Yin Ping Dong, Yao Bai, Feng Qin Li, Xiao Mein Yan, Ai Guo Ma, Jin Xu
OBJECTIVE: To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. METHODS: Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28801381/fluoroquinolone-resistance-mechanisms-by-molecular-epidemiologic-study-of-ciprofloxacin-nonsusceptible-escherichia-coli-sequence-types-isolated-from-clinical-specimens-in-a-tertiary-care-university-hospital-in-korea-emergence-of-clone-st131-2006-2008
#6
Hyung-Seok Yang, Youngjin Kim, Sun Young Cho, You Sun Nam, Tae Sang Oh, Kyung-Sun Park, Hyun-Chul Kim, Hee Joo Lee
To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in gyrA, gyrB, parC, and parE and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28801089/clinical-and-microbiological-characteristics-of-patients-with-bacteremia-caused-by-campylobacter-species-with-an-emphasis-on-the-subspecies-of-c-fetus
#7
Yen-Hung Liu, Wataru Yamazaki, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, Po-Ren Hsueh
OBJECTIVES: This study was intended to investigate the clinical and microbiological characteristics of patients with bacteremia caused by Campylobacter species. METHODS: From April 1998 to May 2014, 56 adults with bacteremia caused by Campylobacter species were evaluated. These Campylobacter species isolates were confirmed to the species level using 16S rRNA gene sequencing (all isolates) and multiplex PCR analysis (for C. fetus only). The performance of identification for Campylobacter species by the Bruker Biotyper MALDI-TOF MS was evaluated...
July 25, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28800821/listeria-monocytogenes-incidence-changes-and-diversity-in-some-brazilian-dairy-industries-and-retail-products
#8
Virginie Oxaran, Sarah Hwa In Lee, Luíza Toubas Chaul, Carlos Humberto Corassin, Giovana Verginia Barancelli, Virgínia Farias Alves, Carlos Augusto Fernandes de Oliveira, Lone Gram, Elaine Cristina Pereira De Martinis
Listeria monocytogenes can cause listeriosis, a severe foodborne disease. In Brazil, despite very few reported cases of listeriosis, the pathogen has been repeatedly isolated from dairies. This has led the government to implement specific legislation to reduce the hazard. Here, we determined the incidence of L. monocytogenes in five dairies and retail products in the Southeast and Midwest regions of Brazil over eight months. Of 437 samples, three samples (0.7%) from retail and only one sample (0.2%) from the dairies were positive for L...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28799835/occurrence-of-carbapenemase-producing-enterobacteriaceae-isolates-in-the-wildlife-first-report-of-oxa-48-in-wild-boars-in-algeria
#9
Taous Bachiri, Sofiane Bakour, Rym Lalaoui, Nadia Belkebla, Meriem Allouache, Jean Marc Rolain, Abdelaziz Touati
The aim of the present study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from wild boars and Barbary macaques in Algeria. Fecal samples were collected from wild boars (n = 168) and Barbary macaques (n = 212), in Bejaia, Algeria, between September 2014 and April 2016. The isolates were identified and antimicrobial susceptibility was determined. Carbapenem resistance determinants were studied using PCR and sequencing, while clonal relatedness was performed using multilocus sequence typing (MLST)...
August 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28783429/characterization-of-livestock-associated-methicillin-resistant-staphylococcus-aureus-cc398-and-mecc-positive-cc130-from-zoo-animals-in-the-united-kingdom
#10
Alessio Bortolami, Ranieri Verin, Julian Chantrey, Michela Corrò, Ian Ashpole, Javier Lopez, Dorina Timofte
Little is known about the characteristics and diseases associated with methicillin-resistant Staphylococcus aureus (MRSA) in nondomestic animals. Four presumptive MRSA isolates, obtained from clinical (n = 3) and surveillance specimens (n = 1) from dwarf (Helogale parvula) and yellow mongooses (Cynictis penicillata) from a United Kingdom zoo, were analyzed by PCR for detection of mecA and mecC-mediated methicillin resistance, and virulence genes. Isolates were genotyped by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) and spa sequence typing...
August 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28783427/molecular-epidemiology-of-carbapenem-resistant-acinetobacter-baumannii-in-a-tertiary-care-hospital-in-egypt-clonal-spread-of-blaoxa-23
#11
Afaf Mohamed Samy El Bannah, Nada Nabil Nawar, Reem Mostafa Mohamed Hassan, Sherifa Tarek Bassiouni Salem
Of great concern is the increased frequency of carbapenem-resistant Acinetobacter baumannii (CRAB) causing healthcare-associated infections. Different classes of β-lactamases are involved in this resistance through hydrolyzing carbapenems. Multilocus sequence typing (MLST) has been applied successfully for characterizing different varieties of bacterial pathogens epidemiologically. In the present study, we aimed to type and characterize the resistance profile of clinical isolates of CRAB causing healthcare-associated infections in patients admitted to Kasr Al-Aini hospital, using MLST, and compare with sequence types (STs) from other countries...
August 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28780272/current-challenges-in-the-accurate-identification-of-streptococcus-pneumoniae-and-its-serogroups-serotypes-in-the-vaccine-era
#12
REVIEW
Rosemol Varghese, Ranjith Jayaraman, Balaji Veeraraghavan
Streptococcus pneumoniae is a major cause of pneumonia, meningitis and other invasive diseases resulting in high mortality and morbidity among children under the age of five. Inaccurate identification of S. pneumoniae masks the exact estimation of disease burden and could delay treatment options. This is the common problem most frequently faced in developing countries due to several reasons that include poor infrastructure, insensitive operational procedures and lack of expertise. Inconsistent methods for phenotypic detection often delay the early identification and confirmation of S...
August 2, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28763660/vancomycin-resistant-enterococci-with-vana-gene-in-treated-municipal-wastewater-and-their-association-with-human-hospital-strains
#13
Veronika Oravcova, Matus Mihalcin, Jana Zakova, Lucie Pospisilova, Martina Masarikova, Ivan Literak
Vancomycin-resistant enterococci (VRE) are pathogens of increasing medical importance. In Brno, Czech Republic, we collected 37 samples from the effluent of a wastewater treatment plant (WWTP), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. Moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the WWTP. Samples were cultured selectively. Enterococci were identified using MALDI-TOF MS, phenotypically tested for susceptibility to antibiotics, and by PCR for occurrence of resistance and virulence genes...
July 27, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28759747/clonal-dissemination-of-vancomycin-resistant-enterococcus-faecium-st412-in-a-brazilian-region
#14
Geraldo da Silva Alves, Monalessa Fábia Pereira, Lais de Lima Bride, Ana Paula Ferreira Nunes, Ricardo Pinto Schuenck
Vancomycin-resistant Enterococcus faecium (VREfm) has emerged as an important global nosocomial pathogen, and this trend is associated with the spread of high-risk clones. Here, we determined the genetic and phenotypic features of 93 VREfm isolates that were obtained from patients in 13 hospitals in Vitória, Espírito Santo, Brazil, during 2012-2013. All the isolates were vancomycin-resistant and harbored the vanA gene. Only 6 (6.5%) of the VREfm isolates showed the ability to form biofilm. The 93 isolates analyzed belong to a single pulsed-field gel electrophoresis lineage and presented six subtypes...
July 28, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28758623/risk-factors-and-predictors-of-carbapenem-resistant-pseudomonas-aeruginosa-and-acinetobacter-baumannii-mortality-in-critically-ill-bacteraemic-patients-over-a-6-year-period-2010-15-antibiotics-do-matter
#15
Matthaios Papadimitriou-Olivgeris, Fotini Fligou, Anastasia Spiliopoulou, Kyriaki Koutsileou, Fevronia Kolonitsiou, Aikaterini Spyropoulou, Anastasia Zotou, Markos Marangos, Evangelos D Anastassiou, Myrto Christofidou, Iris Spiliopoulou
PURPOSE: Acinetobacter baumannii and Pseudomonas aeruginosa provoke serious infections, especially in intensive care unit (ICU) patients. METHODOLOGY: The risk factors and predictors of mortality for P. aeruginosa (n=84; 46 carbapenem-resistant) and A. baumannii (n=129; all carbapenem-resistant) bloodstream infections (BSIs) in an ICU were evaluated. Antibiotic susceptibility testing was performed using the agar disk diffusion method according to EUCAST guidelines...
July 31, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28758109/dissemination-and-molecular-characterization-of-staphylococcus-aureus-at-a-tertiary-referral-hospital-in-xiamen-city-china
#16
Yiwen Yu, Yihui Yao, Qinyun Weng, Jingyi Li, Jianwei Huang, Yiqun Liao, Fu Zhu, Qifeng Zhao, Xu Shen, Jianjun Niu
Staphylococcus aureus is a global epidemic pathogen that causes heavy disease burden. The aim of this study was to determine which globally known S. aureus lineages are currently present in a hospital of Xiamen. Therefore, the 426 S. aureus strains were detected by Melting Curve Analysis (MCA) and genotyped by Pulsed Field Gel Electrophoresis (PFGE) as well as Multicolor Melting Curve Analysis-Based Multilocus Melt Typing (MLMT). In addition, Multilocus Sequence Typing (MLST) was used to identify 108 representative strains...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28757029/diversity-of-streptococcus-equi-subsp-zooepidemicus-strains-isolated-from-the-spanish-sheep-and-goat-population-and-the-identification-function-and-prevalence-of-a-novel-arbutin-utilisation-system
#17
Karen F Steward, Carl Robinson, Matthew T G Holden, Simon R Harris, Ana Fernández Ros, Gema Chacón Pérez, Rafael Baselga, Andrew S Waller
The zoonotic bacterium Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a diverse, opportunistic pathogen that can cause mastitis in dairy sheep and goats. We used multilocus sequence typing (MLST) to define the genetic diversity of 60 isolates of S. zooepidemicus, which were recovered from sheep and goats in Spain between 2003 and 2010. We identify a novel clonal complex based on sequence type (ST), ST-236, which accounted for 39 of the 60 isolates. A representative ST-236 strain, S. zooepidemicus strain C7 (SzC7), was sequenced and interrogated for the presence of novel nutritional uptake or utilisation systems, the acquisition of which have previously been shown to be important for environmental adaptation in other streptococcal pathogens...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28757012/longitudinal-study-of-transmission-of-escherichia-coli-from-broiler-breeders-to-broilers
#18
Louise Ladefoged Poulsen, Ida Thøfner, Magne Bisgaard, Jens Peter Christensen, Rikke Heidemann Olsen, Henrik Christensen
Escherichia coli is of major importance in industrial broiler production as the main cause of salpingitis and peritonitis in broiler breeders. Furthermore E. coli is the most common cause of first week mortality in broiler chickens. The aim of the present study was to investigate the transmission of E. coli, isolated from broiler breeders with salpingitis, to the progeny and the possibility of subsequent first week mortality. Four parent flocks were followed during the whole production period (20-60 weeks) by post mortem and bacteriological examination of randomly selected dead birds...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28756373/genotyping-of-lactobacillus-sanfranciscensis-isolates-from-chinese-traditional-sourdoughs-by-multilocus-sequence-typing-and-multiplex-rapd-pcr
#19
Huanyi Yang, Tongjie Liu, Guohua Zhang, Jiancai Chen, Jingsi Gu, Lei Yuan, Guoqing He
Lactobacillus sanfranciscensis is the predominant lactic acid bacteria (LAB) species in Chinese traditional sourdoughs and conduces to the flavor and rheology properties of Chinese steamed bread, a staple food originated in China over 1500years ago. The aim of this study is to describe the intraspecific diversity of 98 L. sanfranciscensis isolates from 11 Chinese traditional sourdoughs in different regions by multilocus sequence typing (MLST) and multiplex random amplified polymorphic DNA-polymerase chain reaction (multiplex RAPD-PCR)...
July 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28755449/population-structure-and-attribution-of-human-clinical-campylobacter-jejuni-isolates-from-central-europe-to-livestock-and-environmental-sources
#20
J Kovac, B Stessl, N Čadež, I Gruntar, M Cimerman, K Stingl, M Lušicky, M Ocepek, M Wagner, S Smole Možina
Campylobacter jejuni is among the most prevalent causes of human bacterial gastroenteritis worldwide. Domesticated animals and, especially, chicken meat are considered to be the main sources of infections. However, the contribution of surface waters and wildlife in C. jejuni transmission to humans is not well understood. We have evaluated the source attribution potential of a six-gene multiplex PCR (mPCR) method coupled with STRUCTURE analysis on a set of 410 C. jejuni strains isolated from environment, livestock, food and humans in central Europe...
July 28, 2017: Zoonoses and Public Health
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