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https://www.readbyqxmd.com/read/29157313/calpain-5-gene-expression-in-the-mouse-eye-and-brain
#1
Kellie Schaefer, MaryAnn Mahajan, Anuradha Gore, Stephen H Tsang, Alexander G Bassuk, Vinit B Mahajan
OBJECTIVE: Our objective was to characterize CAPN5 gene expression in the mouse central nervous system. Mouse brain and eye sections were probed with two high-affinity RNA oligonucleotide analogs designed to bind CAPN5 RNA and one scramble, control oligonucleotide. Images were captured in brightfield. RESULTS: CAPN5 RNA probes were validated on mouse breast cancer tumor tissue. In the eye, CAPN5 was expressed in the ganglion cell, inner nuclear and outer nuclear layers of the retina...
November 21, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29152136/postnatal-calpain-inhibition-elicits-cerebellar-cell-death-and-motor-dysfunction
#2
Junyao Li, Sanjuan Yang, Guoqi Zhu
Calpain-1 deletion elicits neurodevelopmental disorders, such as ataxia. However, the function of calpain in postnatal neurodevelopment and its mechanisms remain unknown. In this study, we revealed that postnatal intraperitoneal injection of various calpain inhibitors attenuated cerebellar cytosolic calpain activity. Moreover, postnatal application of calpeptin (2 mg/kg) apparently reduced spectrin breakdown, promoted suprachiasmatic nucleus circadian oscillatory protein (SCOP) accumulation in cerebellar tissue...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151334/investigation-of-glucocorticoid-receptor-and-calpain-10-gene-polymorphisms-in-turkish-patients-with-type-2-diabetes-mellitus
#3
Naciye Selcen Bayramcı, Leyla Açık, Çağdaş Kalkan, İlhan Yetkin
Background/aim: We proposed to investigate the role of calpain-10 (CAPN10) gene single nucleotide polymorphism (SNP)-19 and SNP-44 and glucocorticoid receptor (NR3C1) gene N363S polymorphisms in Turkish patients with type 2 diabetes mellitus (T2DM).Materials and methods: Peripheral blood samples were obtained from 125 patients with T2DM and 112 healthy volunteers. Genotyping was carried out by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: There were no statistically significant differences found between the allele and genotype frequencies of CAPN10 SNP-19, CAPN10 SNP-44, and NR3C1 N363S polymorphisms and T2DM development (P > 0...
November 13, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/29151105/eryptosis-the-neglected-cause-of-anemia-in-end-stage-renal-disease
#4
Florian Lang, Rosi Bissinger, Majed Abed, Ferruh Artunc
End stage renal disease (ESRD) invariably leads to anemia which has been mainly attributed to compromised release of erythropoietin from the defective kidneys with subsequent impairment of erythropoiesis. However, erythropoietin replacement only partially reverses anemia pointing to the involvement of additional mechanisms. As shown more recently, anemia of ESRD is indeed in large part a result of accelerated erythrocyte loss due to suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the cell surface...
October 23, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29150934/the-current-status-and-perspectives-regarding-the-clinical-implication-of-intracellular-calcium-in-breast-cancer
#5
REVIEW
Amir Tajbakhsh, Alireza Pasdar, Mehdi Rezaee, Mostafa Fazeli, Saman Soleimanpour, Seyed Mahdi Hassanian, Zahra FarshchiyanYazdi, Tayebe Younesi Rad, Gordon A Ferns, Amir Avan
Calcium ions (Ca(2+) ) act as second messengers in intracellular signaling. Ca(2+) pumps, channels, sensors, and calcium binding proteins, regulate the concentrations of intracellular Ca(2+) as a key regulator of important cellular processes such as gene expression, proliferation, differentiation, DNA repair, apoptosis, metastasis, and hormone secretion. Intracellular Ca(2+) also influences the functions of several organelles, that include: the endoplasmic reticulum, mitochondria, the Golgi, and cell membrane both in normal and breast cancer cells...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29149631/prostaglandin-j2-promotes-o-glcnacylation-raising-app-processing-by-%C3%AE-and-%C3%AE-secretases-relevance-to-alzheimer-s-disease
#6
Teneka Jean-Louis, Patricia Rockwell, Maria E Figueiredo-Pereira
Regulation of the amyloid precursor protein (APP) processing by α- and β-secretases is of special interest to Alzheimer's disease (AD), as these proteases prevent or mediate amyloid beta formation, respectively. Neuroinflammation is also implicated in AD. Our data demonstrate that the endogenous mediator of inflammation prostaglandin J2 (PGJ2) promotes full-length APP (FL-APP) processing by α- and β-secretases. The decrease in FL-APP was independent of proteasomal, lysosomal, calpain, caspase, and γ-secretase activities...
November 14, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/29145487/defining-external-factors-that-determine-neuronal-survival-apoptosis-and-necrosis-during-excitotoxic-injury-using-a-high-content-screening-imaging-platform
#7
Ujval Anilkumar, Petronela Weisova, Jasmin Schmid, Tytus Bernas, Heinrich J Huber, Heiko Düssmann, Niamh M C Connolly, Jochen H M Prehn
Cell death induced by excessive glutamate receptor overactivation, excitotoxicity, has been implicated in several acute and chronic neurological disorders. While numerous studies have demonstrated the contribution of biochemically and genetically activated cell death pathways in excitotoxic injury, the factors mediating passive, excitotoxic necrosis are less thoroughly investigated. To address this question, we developed a high content screening (HCS) based assay to collect high volumes of quantitative cellular imaging data and elucidated the effects of intrinsic and external factors on excitotoxic necrosis and apoptosis...
2017: PloS One
https://www.readbyqxmd.com/read/29133537/expression-of-concern-for-takano-et-al-vital-role-of-the-calpain-calpastatin-system-for-placental-integrity-dependent-embryonic-survival
#8
Jiro Takano, Naomi Mihira, Ryo Fujioka, Emi Hosoki, Athar H Chishti, Takaomi C Saido
No abstract text is available yet for this article.
December 1, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29126932/docosahexaenoic-acid-dha-enhances-the-therapeutic-potential-of-neonatal-neural-stem-cell-transplantation-post-traumatic-brain-injury
#9
Hussein Ghazale, Naify Ramadan, Sara Mantash, Kazem Zibara, Sally El-Sitt, Hala Darwish, Farah Chamaa, Rose Mary Boustany, Stefania Mondello, Wassim Abou-Kheir, Jihane Soueid, Firas Kobeissy
Traumatic Brain Injury (TBI) is a major cause of death and disability worldwide with 1.5 million people inflicted yearly. Several neurotherapeutic interventions have been proposed including drug administration as well as cellular therapy involving neural stem cells (NSCs). Among the proposed drugs is docosahexaenoic acid (DHA), a polyunsaturated fatty acid, exhibiting neuroprotective properties. In this study, we utilized an innovative intervention of neonatal NSCs transplantation in combination with DHA injections in order to ameliorate brain damage and promote functional recovery in an experimental model of TBI...
November 7, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29124934/can-relative-binding-free-energy-predict-selectivity-of-reversible-covalent-inhibitors
#10
Payal Chatterjee, Wesley M Botello-Smith, Han Zhang, Li Qian, Abdelaziz Alsamarah, David Kent, Jerome J Lacroix, Michel Baudry, Yun Luo
Reversible covalent inhibitors have many clinical advantages over noncovalent or covalent drugs. However, apart from selecting a warhead, substantial efforts in design and synthesis are needed to optimize noncovalent interactions to improve target-selective binding. Computational prediction of binding affinity for reversible covalent inhibitors presents a unique challenge since the binding process consists of multiple steps, which are not necessarily independent of each other. In this study, we lay out the relation between relative binding free energy and the overall reversible covalent binding affinity using a two-state binding model...
November 10, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29115452/mir-93%C3%A2-mediated-collagen-expression-in-stress-urinary-incontinence-via-calpain-2
#11
Shi-Jie Yang, Jian Wang, Jie Xu, Yun Bai, Zhao-Jun Guo
The aim of the present study was to investigate the expression and mechanism of microRNA (miR)‑93 in collagen expression in stress urinary incontinence (SUI). Vaginal tissue, primary fibroblasts and SUI primary fibroblasts were obtained to detect the expression of miR‑93, interstitial collagenase (MMP1), collagen I and calpain‑2. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect the levels of miR‑93 and MMP1. Western blotting was used to evaluate the protein levels of calpain‑2, MMP1 and collagen I...
October 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29112674/interleukin-10-and-pd150606-modulate-expression-of-ampa-receptor-glua1-and-glua2-subunits-under-hypoxic-conditions
#12
Sergei G Levin, Nikolai P Sirota, Miroslav N Nenov, Tatyana A Savina, Oleg V Godukhin
The goal of this study was to evaluate the effects of anti-inflammatory cytokine, interleukin-10 (IL-10), and calpain inhibitor, PD150606, on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits in rat hippocampal slices exposed to repeated brief hypoxic episodes. We studied both individual and combinatory effects of PD150606 and IL-10 on the expression of AMPA receptor subunits under hypoxic conditions for GluA1 and GluA2 as well as their phosphorylated forms - pSer831-GluA1 and pSer880-GluA2...
November 6, 2017: Neuroreport
https://www.readbyqxmd.com/read/29111855/neuroprotective-effects-of-inhibitors-of-acid-sensing-ion-channels-asics-in-optic-nerve-crush-model-in-rodents
#13
Dorota L Stankowska, Brett H Mueller, Hidehiro Oku, Tsunehiko Ikeda, Adnan Dibas
PURPOSE: The purpose of the current study was to assess the potential involvement of acid-sensing ion channel 1 (ASIC1) in retinal ganglion cell (RGC) death and investigate the neuroprotective effects of inhibitors of ASICs in promoting RGC survival following optic nerve crush (ONC). RESULTS: ASIC1 protein was significantly increased in optic nerve extracts at day 7 following ONC in rats. Activated calpain-1 increased at 2 and 7 days following ONC as evidenced by increased degradation of α-fodrin, known substrate of calpain...
November 7, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29111403/the-critical-role-of-autophagy-in-cadmium-induced-immunosuppression-regulated-by-endoplasmic-reticulum-stress-mediated-calpain-activation-in-raw264-7-mouse-monocytes
#14
Keum-Young So, Byung-Hoon Lee, Seon-Hee Oh
Cadmium (Cd) has toxic and suppressive effects on the immune system, but the underlying mechanisms remain poorly understood. Here, we show that autophagy plays a critical role in regulation of Cd-induced immunosuppression in RAW264.7 cells. Cd decreased cell viability in a dose-dependent manner; cleaved caspase-8, caspase-3, and poly (ADP-ribose) polymerase (PARP)-1; increased DNA laddering; induced CCAAT-enhancer-binding protein homologous protein (CHOP); and reduced tumor necrosis factor (TNF)-α expression; indicating that caspase-dependent and endoplasmic reticulum (ER)-mediated apoptosis are involved in Cd-induced immunotoxicity...
October 27, 2017: Toxicology
https://www.readbyqxmd.com/read/29110181/c-myb-plays-an-essential-role-in-the-protective-function-of-igf-1-on-cytotoxicity-induced-by-a%C3%AE-25-35-via-the-pi3k-akt-pathway
#15
Jingyu Zhang, Yongwei Shu, Yang Qu, Lina Zhang, Tingting Chu, Yonghui Zheng, Hong Zhao
There have been numerous reports about neurodegenerative diseases, including Alzheimer's disease. Nevertheless, the molecules responsible for the neurodegeneration in Alzheimer's disease are basically unknown. Recent findings indicate that the cellular myeloblastosis (c-myb) regulates neural progenitor cell proliferation. In the current study, the function of insulin-like growth factor-1 (IGF-1) against cell toxicity in SH-SY5Y cells induced by β-amyloid 25-35 (Aβ25-35) and its molecular mechanism were investigated...
November 6, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29107867/application-of-high-temperature-14%C3%A2-c-aging-of-beef-m-semimembranosus-with-low-dose-electron-beam-and-x-ray-irradiation
#16
So Yeon Kim, Hae In Yong, Ki Chang Nam, Samooel Jung, Dong-Gyun Yim, Cheorun Jo
The effects of irradiation source (electron beam [EB] and X-ray [XR]), aging temperature (4°C and 14°C), and aging time (0, 3, 7, and 14days) were evaluated on microbial quality, physicochemical properties, and calpain-1 autolysis in beef M. semimembranosus. Regardless of irradiation source, irradiation prior to aging reduced the total number of aerobic bacteria in beef and this reduction was maintained during aging. Irradiation did not affect the pH, b(⁎) value, shear force, or myofibrillar fragmentation index of beef at day 0...
November 3, 2017: Meat Science
https://www.readbyqxmd.com/read/29107090/inhibition-of-angiotensin-ii-and-calpain-attenuates-pleural-fibrosis
#17
Lin-Jie Song, Fei Xiang, Hong Ye, Hai Huang, Jie Yang, Fan Yu, Liang Xiong, Juan-Juan Xu, Peter A Greer, Huan-Zhong Shi, Jian-Bao Xin, Yunchao Su, Wan-Li Ma
Pleural fibrosis is associated with various inflammatory processes such as tuberculous pleurisy and bacterial empyema. There is currently no ideal therapeutic to attenuate pleural fibrosis. Some pro-fibrogenic mediators induce fibrosis through inflammatory processes, suggesting that blockage of these mediators might prevent pleural fibrosis. The MeT-5A human pleural mesothelial cell line (PMC) was used in this study as an in vitro model of fibrosis; and intra-pleural injection of bleomycin with carbon particles was used as an in vivo mouse model of pleural fibrosis...
October 26, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29106383/s-sulfocysteine-nmda-receptor-dependentsignaling-underlies-neurodegeneration-in-molybdenum-cofactor-deficiency
#18
Avadh Kumar, Borislav Dejanovic, Florian Hetsch, Marcus Semtner, Debora Fusca, Sita Arjune, Jose Angel Santamaria-Araujo, Aline Winkelmann, Scott Ayton, Ashley I Bush, Peter Kloppenburg, Jochen C Meier, Guenter Schwarz, Abdel Ali Belaidi
Molybdenum cofactor deficiency (MoCD) is an autosomal recessive inborn error of metabolism characterized by neurodegeneration and death in early childhood. The rapid and progressive neurodegeneration in MoCD presents a major clinical challenge and may relate to the poor understanding of the molecular mechanisms involved. Recently, we reported that treating patients with cyclic pyranopterin monophosphate (cPMP) is a successful therapy for a subset of infants with MoCD and prevents irreversible brain damage. Here, we studied S-sulfocysteine (SSC), a structural analog of glutamate that accumulates in the plasma and urine of patients with MoCD, and demonstrated that it acts as an N-methyl D-aspartate receptor (NMDA-R) agonist, leading to calcium influx and downstream cell signaling events and neurotoxicity...
November 6, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29104429/role-of-melatonin-in-reducing-amphetamine-induced-degeneration-in-substantia-nigra-of-rats-via-calpain-and-calpastatin-interaction
#19
Jirapa Chetsawang, Sujira Mukda, Rachneekorn Srimokra, Piyarat Govitrapong, Banthit Chetsawang
Excessive intracellular calcium levels induce calpain activation, thereby triggering the cell death cascade. Several lines of evidence have demonstrated the neuroprotective role of the overexpression of calpain inhibitor, calpastatin. In this study, amphetamine-induced degeneration in the substantia nigra of rats was determined by evaluating the decrease in the levels of tyrosine hydroxylase phosphorylation. Amphetamine significantly decreased calpastatin levels but increased calpain levels. An induction in calpain activity was demonstrated by an increase in the formation of calpain spectrin breakdown products...
2017: Journal of Experimental Neuroscience
https://www.readbyqxmd.com/read/29104086/an-easy-to-use-fret-protein-substrate-to-detect-calpain-cleavage-in-vitro-and-in-vivo
#20
Christian-Scott E McCartney, James A MacLeod, Peter A Greer, Peter L Davies
Calpain-1 and -2 are Ca(2+)-activated intracellular cysteine proteases that regulate a wide range of cellular functions through the cleavage of their protein substrates. Unlike degradative proteases, calpains make limited, transformative cleavages, typically in accessible sequences linking discrete subdomains, to irreversibly alter substrate functions. The biological roles of calpain and their interplay with calcium signaling are of significant biomedical interest as biomarkers and potential therapeutic targets in a growing number of diseases including Alzheimer's, cancer and fibrosis...
November 14, 2017: Biochimica et Biophysica Acta
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