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Bioprocessing

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https://www.readbyqxmd.com/read/28212019/complete-nutrient-removal-coupled-to-nitrous-oxide-production-as-a-bioenergy-source-by-denitrifying-polyphosphate-accumulating-organisms
#1
Han Gao, Miaomiao Liu, James S Griffin, Longcheng Xu, Da Xiang, Yaniv D Scherson, Wen-Tso Liu, George F Wells
Coupled Aerobic-anoxic Nitrous Decomposition Operation (CANDO) is a promising emerging bioprocess for wastewater treatment that enables direct energy recovery from nitrogen (N) in three steps: (1) ammonium oxidation to nitrite; (2) denitrification of nitrite to nitrous oxide (N2O); and (3) N2O conversion to N2 with energy generation. However, CANDO does not currently target phosphorus (P) removal. Here, we demonstrate that denitrifying polyphosphate accumulating organism (PAO) enrichment cultures are capable of catalyzing simultaneous biological N and P removal coupled to N2O generation in a 2nd generation CANDO process, CANDO+P...
February 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28206756/effect-of-bioprocessing-on-the-in-vitro-colonic-microbial-metabolism-of-phenolic-acids-from-rye-bran-fortified-breads
#2
Ville Mikael Koistinen, Emilia Nordlund, Kati Katina, Ismo Mattila, Kaisa Poutanen, Kati Hanhineva, Anna-Marja Aura
Cereal bran is an important source of dietary fiber and bioactive compounds, such as phenolic acids. We aimed to study the phenolic acid metabolism of native and bioprocessed rye bran fortified refined wheat bread and to elucidate the microbial metabolic route of phenolic acids. After incubation in an in vitro colon model, the metabolites were analyzed using two different methods applying mass spectrometry. While phenolic acids were released more extensively from the bioprocessed bran bread and ferulic acid had consistently higher concentrations in the bread type during fermentation, there were only minor differences in the appearance of microbial metabolites, including the diminished levels of certain phenylacetic acids in the bioprocessed bran...
February 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28205239/bioprocessing-of-common-beans-in-diets-for-tilapia-in-vivo-digestibility-and-antinutritional-factors
#3
Francisco Valdez-González, Roberto Gutiérrez-Dorado, Alfredo Hernández-Llamas, Manuel García-Ulloa, Luís Sánchez-Magaña, Breidy Cuevas-Rodríguez, Hervey Rodríguez-González
BACKGROUND: Bioprocessing of ingredients by solid-state fermentation is a low-cost technique for preparing diets. It is perfomed by adding microorganisms as Rhizopus oligosporus to the grains of bean, with minimal degradation of nutrients, and a significant improvement in digestibility. In particular, fermentation induces favorable changes in beans by reducing enzyme inhibitors, such as phytates and tannins. RESULTS: Fermentation significantly (P < 0.05) increased the protein content and digestibility of dry matter and protein compared with whole bean grains, and decreased the content of lipids, ash, and phytic acid...
February 16, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28193276/thermus-thermophilus-as-source-of-thermozymes-for-biotechnological-applications-homologous-expression-and-biochemical-characterization-of-an-%C3%AE-galactosidase
#4
Martina Aulitto, Salvatore Fusco, Gabriella Fiorentino, Danila Limauro, Emilia Pedone, Simonetta Bartolucci, Patrizia Contursi
BACKGROUND: The genus Thermus, which has been considered for a long time as a fruitful source of biotechnological relevant enzymes, has emerged more recently as suitable host to overproduce thermozymes. Among these, α-galactosidases are widely used in several industrial bioprocesses that require high working temperatures and for which thermostable variants offer considerable advantages over their thermolabile counterparts. RESULTS: Thermus thermophilus HB27 strain was used for the homologous expression of the TTP0072 gene encoding for an α-galactosidase (TtGalA)...
February 13, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28188692/bioreactor-scale-up-and-protein-product-quality-characterization-of-piggybac-transposon-derived-cho-pools
#5
Yashas Rajendra, Sowmya Balasubramanian, Robert B Peery, James R Swartling, Neil A McCracken, Dawn L Norris, Christopher C Frye, Gavin C Barnard
Chinese hamster ovary (CHO) cells remain the most popular host for the production of biopharmaceutical drugs, particularly monoclonal antibodies (mAbs), bispecific antibodies and Fc-fusion proteins. Creating and characterizing the stable CHO clonally-derived cell lines (CDCLs) needed to manufacture these therapeutic proteins is a lengthy and laborious process. Therefore, CHO pools have increasingly been used to rapidly produce protein to support and enable preclinical drug development. We recently described the generation of CHO pools yielding mAb titers as high as 7...
February 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28185098/advancement-in-bioprocess-technology-parallels-between-microbial-natural-products-and-cell-culture-biologics
#6
REVIEW
Arpan A Bandyopadhyay, Anurag Khetan, Li-Hong Malmberg, Weichang Zhou, Wei-Shou Hu
The emergence of natural products and industrial microbiology nearly eight decades ago propelled an era of bioprocess innovation. Half a century later, recombinant protein technology spurred the tremendous growth of biologics and added mammalian cells to the forefront of industrial producing cells in terms of the value of products generated. This review highlights the process technology of natural products and protein biologics. Despite the separation in time, there is a remarkable similarity in their progression...
February 9, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28178596/automated-2d-hplc-method-for-characterization-of-protein-aggregation-with-in-line-fraction-collection-device
#7
Abasha Williams, Erik K Read, Cyrus D Agarabi, Scott Lute, Kurt A Brorson
Monoclonal antibodies are mainly produced by mammalian cell culture, which due to its complexity, results in a wide range of product variants/isoforms. With the growing implementation of Quality by Design (QbD) and Process Analytical Technology (PAT) in drug manufacturing, monitoring and controlling quality attributes within a predefined range during manufacturing may provide added consistency to product quality. To implement these concepts, more robust analytical tools could reduce the time needed for monitoring quality attributes during upstream processing...
January 25, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28176458/serum-free-bioprocessing-of-adult-human-and-rodent-skin-derived-schwann-cells-implications-for-cell-therapy-in-nervous-system-injury
#8
Leila Mirfeizi, Jo Anne Stratton, Ranjan Kumar, Prajay Shah, Natacha Agabalyan, Morgan G Stykel, Rajiv Midha, Jeff Biernaskie, Michael S Kallos
Peripheral nerve injury affects 2.8% of trauma patients with severe cases often resulting in long-lived permanent disability, despite nerve repair surgery. Autologous Schwann cell (SC) therapy currently provides an exciting avenue for improved outcomes for these patients, particularly with the possibility to derive SCs from easily-accessible adult skin. However, due to current challenges regarding the efficient expansion of these cells, further optimization is required before they can be seriously considered for clinical application...
February 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28174039/bioprocess-integration-for-human-mesenchymal-stem-cells-from-up-to-downstream-processing-scale-up-to-cell-proteome-characterization
#9
Bárbara Cunha, Tiago Aguiar, Sofia B Carvalho, Marta M Silva, Ricardo A Gomes, Manuel J T Carrondo, Patrícia Gomes-Alves, Cristina Peixoto, Margarida Serra, Paula M Alves
To deliver the required cell numbers and doses to therapy, scaling-up production and purification processes (at least to the liter-scale) while maintaining cells' characteristics is compulsory. Therefore, the aim of this work was to prove scalability of an integrated streamlined bioprocess compatible with current good manufacturing practices (cGMP) comprised by cell expansion, harvesting and volume reduction unit operations using human mesenchymal stem cells (hMSC) isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC)...
February 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28169054/addition-of-cattle-manure-to-sheep-bedding-allows-vermicomposting-process-and-improves-vermicompost-quality
#10
Taiana Cestonaro, Mônica Sarolli Silva de Mendonça Costa, Luiz Antonio de Mendonça Costa, Dercio Ceri Pereira, Marcos A T Rozatti, Marcos F Leal Martins
Animal waste is usually a good substrate for vermicomposting. However, numerous animal husbandry systems use bedding that consists primarily of lignocellulosic substrates, which hinders earthworm and microorganism's development and thus, the entire bioconversion process. One possible solution is to mix the used bedding with other waste materials that are more amenable to earthworm ingestion and can provide better conditions for earthworm population growth. Here, we have aimed to examine the effectiveness of such procedure by mixing rice-husk-based sheep bedding with cattle manure in different proportions (0%, 25%, 50%, 75% and 100%)...
February 4, 2017: Waste Management
https://www.readbyqxmd.com/read/28167112/combining-molecular-and-bioprocess-techniques-to-produce-poly-3-hydroxybutyrate-co-3-hydroxyhexanoate-with-controlled-monomer-composition-by-burkholderia-sacchari
#11
Thatiane T Mendonça, Rafaela R Tavares, Lucas G Cespedes, Ruben J Sánchez-Rodriguez, Jan Schripsema, Marilda K Taciro, José G C Gomez, Luiziana F Silva
Biopolymers as polyhydroxyalkanoates (PHA) composed by different co-monomers 3-hydroxybutyrate and 3-hydroxyhexanoate [P(3HB-co-3HHx)] has attracted interest since its properties are similar to low density polyethylene. Burkholderia sacchari produces this copolymer with a very low 3HHx molar fraction, about 2 mol%. B. sacchari mutant (unable to produce polymer) was engineered to host PHA biosynthesis genes (phaPCJ) from Aeromonas sp. In addition, a two-step bioprocess to increase biopolymer production was developed...
February 3, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28165055/human-embryonic-and-induced-pluripotent-stem-cells-maintain-phenotype-but-alter-their-metabolism-after-exposure-to-rock-inhibitor
#12
Spyros I Vernardis, Konstantinos Terzoudis, Nicki Panoskaltsis, Athanasios Mantalaris
Human pluripotent stem cells (hPSCs) are adhesion-dependent cells that require cultivation in colonies to maintain growth and pluripotency. Robust differentiation protocols necessitate single cell cultures that are achieved by use of ROCK (Rho kinase) inhibitors. ROCK inhibition enables maintenance of stem cell phenotype; its effects on metabolism are unknown. hPSCs were exposed to 10 μM ROCK inhibitor for varying exposure times. Pluripotency (TRA-1-81, SSEA3, OCT4, NANOG, SOX2) remained unaffected, until after prolonged exposure (96 hrs)...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28163783/a-framework-for-accelerated-phototrophic-bioprocess-development-integration-of-parallelized-microscale-cultivation-laboratory-automation-and-kriging-assisted-experimental-design
#13
Holger Morschett, Lars Freier, Jannis Rohde, Wolfgang Wiechert, Eric von Lieres, Marco Oldiges
BACKGROUND: Even though microalgae-derived biodiesel has regained interest within the last decade, industrial production is still challenging for economic reasons. Besides reactor design, as well as value chain and strain engineering, laborious and slow early-stage parameter optimization represents a major drawback. RESULTS: The present study introduces a framework for the accelerated development of phototrophic bioprocesses. A state-of-the-art micro-photobioreactor supported by a liquid-handling robot for automated medium preparation and product quantification was used...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28160134/bacterial-and-fungal-proteolytic-enzymes-production-catalysis-and-potential-applications
#14
REVIEW
Ronivaldo Rodrigues da Silva
Submerged and solid-state bioprocesses have been extensively explored worldwide and employed in a number of important studies dealing with microbial cultivation for the production of enzymes. The development of these production technologies has facilitated the generation of new enzyme-based products with applications in pharmaceuticals, food, bioactive peptides, and basic research studies, among others. The applicability of microorganisms in biotechnology is potentiated because of their various advantages, including large-scale production, short time of cultivation, and ease of handling...
February 3, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28160118/production-of-chemicals-from-c1-gases-co-co2-by-clostridium-carboxidivorans
#15
REVIEW
Ánxela Fernández-Naveira, Haris Nalakath Abubackar, María C Veiga, Christian Kennes
Bioprocesses in conventional second generation biorefineries are mainly based on the fermentation of sugars obtained from lignocellulosic biomass or agro-industrial wastes. An alternative to this process consists in gasifying those same feedstocks or even other carbon-containing materials to obtain syngas which can also be fermented by some anaerobic bacteria to produce chemicals or fuels. Carbon monoxide, carbon dioxide and hydrogen, which are the main components of syngas, are also found in some industrial waste gases, among others in steel industries...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28159788/revisiting-the-regulation-of-the-primary-scaffoldin-gene-in-clostridium-thermocellum
#16
Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Edward A Bayer, Yuval Shoham, Raphael Lamed, Ilya Borovok
: Cellulosomes are considered to be one of the most efficient systems for degradation of plant cell-wall polysaccharides. The central cellulosome component comprises a large, noncatalytic, protein subunit called scaffoldin. Multiple saccharolytic enzymes are incorporated into the scaffoldins via specific high-affinity cohesin-dockerin interactions. Recently, the regulation of genes encoding certain cellulosomal components by multiple RNA polymerase alternative σ(I) factors has been demonstrated in Clostridium (Ruminiclostridium) thermocellum In the present report, we provide experimental evidence demonstrating that the C...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28150747/methanol-independent-protein-expression-by-aox1-promoter-with-trans-acting-elements-engineering-and-glucose-glycerol-shift-induction-in-pichia-pastoris
#17
Jinjia Wang, Xiaolong Wang, Lei Shi, Fei Qi, Ping Zhang, Yuanxing Zhang, Xiangshan Zhou, Zhiwei Song, Menghao Cai
The alcohol oxidase 1 promoter (PAOX1) of Pichia pastoris is commonly used for high level expression of recombinant proteins. While the safety risk of methanol and tough process control for methanol induction usually cause problems especially in large-scale fermentation. By testing the functions of trans-acting elements of PAOX1 and combinatorially engineering of them, we successfully constructed a methanol-free PAOX1 start-up strain, in which, three transcription repressors were identified and deleted and, one transcription activator were overexpressed...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28150192/establishment-of-bioprocess-for-synthesis-of-nicotinamide-by-recombinant-escherichia-coli-expressing-high-molecular-mass-nitrile-hydratase
#18
Zhe Wang, Zhongmei Liu, Wenjing Cui, Zhemin Zhou
Application of engineered bacteria expressing nitrile hydratase for the production of amide is getting tremendous attention due to the rapid development of recombinant DNA technique. This study evaluated the effect of 3-cyanopyridine concentrations on nicotinamide production using recombinant Escherichia coli strain (BAG) expressing high-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1, and established proper process of whole-cell catalysis of 3-cyanopyridine and high cell-density cultivation...
February 1, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28144742/comment-on-elucidating-the-binding-efficacy-of-%C3%AE-galactosidase-on-graphene-by-docking-approach-and-its-potential-application-in-galacto-oligosaccharide-production
#19
Marek Wiśniewski, Katarzyna Roszek
In a recent study, Satar et al. (Bioprocess Biosyst Eng 39:807-814, 2016) have reported "the synthesis and characterization of graphene for the immobilization of β-galactosidase for improved galacto-oligosaccharide (GOS) production". There are several issues in this study that must be commented.
January 31, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28143659/enantio-convergent-biotransformation-of-o-o-dimethyl-4-oxoazetidin-2-ylphosphonate-using-fungal-cells-of-penicillium-minioluteum-and-purified-enzymes
#20
Natalia Kmiecik, Ewa Żymańczyk-Duda
This report presents the bioconversion of O,O-dimethyl-4-oxoazetidin-2-ylphosphonate 1 performed in two ways: with the enzymatic system of P. minioluteum and with the application of purified enzymes: penicillinase and two proteases of different origin. Recorded NMR spectra allowed confirming the reaction progress and also postulating possible mechanism of conversion. The path of bioconversion was defined as enantio convergent process for both modes of applied biocatalysts. This means that kinetically driven resolution of racemic mixture of the substrate leads to the one enantiomer of the product...
January 24, 2017: Bioorganic Chemistry
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