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G Jayanath, Sowmya P Mohandas, Bhavya Kachiprath, Solly Solomon, T P Sajeevan, I S Bright Singh, Rosamma Philip
A novel esterase, designated as EstSP was identified by function based screening from a soil metagenomic fosmid library of solar saltern of Goa. EstSP gene of 1065 bp encoding a putative esterase of 354 amino acids showing 55% identity to esterase from gamma proteobacterium HIMB55 was identified. The enzyme EstSP belongs to family IV hormone sensitive lipase with novel sequence characteristics and a unique motif GDSGG. EstSP was expressed in Escherichia coli and purified protein with molecular mass of 58...
June 13, 2018: International Journal of Biological Macromolecules
Kenichi Ogawa, Katsunori Yoshikawa, Fumio Matsuda, Yoshihiro Toya, Hiroshi Shimizu
Photoinhibition, or cell damage caused by excessively intense light is a major issue for the industrial use of cyanobacteria. To investigate the mechanism of responses to extreme high light intensity, gene expression analysis was performed using the model cyanobacterium Synechocystis sp. PCC 6803 (PCC 6803) cultured under various light intensities. The culture profile data demonstrated that, in contrast to the slow cell growth observed under low light intensities (30 and 50 μmol m-2  s-1 ), maximal cell growth was observed under mid light conditions (300 and 1000 μmol m-2  s-1 )...
June 12, 2018: Journal of Bioscience and Bioengineering
Qiuzhen Wang, Huike Ye, Biswarup Sen, Yunxuan Xie, Yaodong He, Sunghoon Park, Guangyi Wang
Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains. Two different thraustochytrid strains, Schizochytrium sp. PKU#Mn4 and Thraustochytriidae sp. PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions. Although they exhibited similar fatty acid profiles, PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions...
June 2018: Synthetic and Systems Biotechnology
Sophie Zhao, Ian Walsh, Jodie L Abrahams, Louise Royle, Terry Nguyen-Khuong, Daniel Spencer, Daryl L Fernandes, Nicolle H Packer, Pauline M Rudd, Matthew P Campbell
Summary: GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics. The database is built on publicly available experimental data sets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT). It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd...
April 20, 2018: Bioinformatics
Daniel G Greene, Steven J Traylor, Jing Guo, Leila H Choe, Shannon Modla, Xuankuo Xu, Nripen Singh, Lye Lin Lock, Sanchayita Ghose, Zheng Jian Li, Kelvin H Lee, Norman J Wagner, Abraham M Lenhoff
Protein precipitates that arise during bioprocessing can cause manufacturing challenges but they can also aid in clearance of host-cell protein and DNA impurities. Such precipitates differ from many protein precipitates that have been studied previously in their heterogeneous composition, particularly in the presence of high concentrations of the product protein. Here we characterize the precipitates that form after neutralization of Protein A purified and viral-inactivated material of an Fc-fusion protein produced in Chinese hamster ovary (CHO) cells...
June 13, 2018: Biotechnology and Bioengineering
João F Gomes, Ana Lopes, Daniel Gonçalves, Cristina Luxo, Marta Gmurek, Raquel Costa, Rosa M Quinta-Ferreira, Rui C Martins, Ana Matos
Corbicula fluminea, an Asian clam, is one of the worst invasive species in Europe that can survive in very adverse environmental conditions. Despite its negative impacts, the species also has the capacity to bioaccumulate heavy metals, contaminants and can be exploited for wastewater treatment purposes. The capacity of the Asian clam to remove Escherichia coli, used as fecal contamination indicator, was analyzed. Conventional wastewater treatment plants are not suitable to remove bacteria, thus resulting in treated municipal wastewater with high bacterial loads...
June 6, 2018: Chemosphere
Bence Kozma, András Salgó, Szilveszter Gergely
In-situ Raman spectroscopy is frequently applied to monitor and even control the glucose concentration of monoclonal antibody producing mammalian cell cultivations. Previous studies used the PLSR algorithm only, however other multivariate algorithms were applied successfully for different protein production processes. In this study, four mammalian cell cultivation runs were followed with Raman spectroscopy and the spectra were analysed quantitatively and qualitatively as well. The PCA analysis showed that one of the most dominant factors in the Raman spectra were the concentration of glucose, which strongly correlated with the score values of the eighth principal component...
June 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Claudia Bernal, Karen Rondriguez, Ronny Martínez
Enzyme immobilization often achieves reusable biocatalysts with improved operational stability and solvent resistance. However, these modifications are generally associated with a decrease in activity or detrimental modifications in catalytic properties. On the other hand, protein engineering aims to generate enzymes with increased performance at specific conditions by means of genetic manipulation, directed evolution and rational design. However, the achieved biocatalysts are generally generated as soluble enzymes, -thus not reusable- and their performance under real operational conditions is uncertain...
June 9, 2018: Biotechnology Advances
Mohammad H Morowvat, Younes Ghasemi
BACKGROUND: The photosynthetic unicellular microalgal strain, Chlorella vulgaris is considered as a robust biomass and lipid producer for human food and feed and also green energy production. The culture media composition plays a crucial role in biomass and lipid production and productivity. METHODS: The photosynthetic green microalga C. vulgaris was studied in three distinct cultivation experiments each for 22 days, using BG11 and f/2 culture media to investigate the effects of different levels of nitrogen and culture media replacement on cell growth, lipid production and productivity...
June 10, 2018: Recent Patents on Food, Nutrition & Agriculture
Alejandro González-Mora, Federico Ruiz-Ruiz, Jorge Benavides, Marco Rito-Palomares
Aqueous two-phase systems (ATPS) have been widely exploited for the recovery and partial purification of biological compounds. Recently our research group characterized the primary recovery and partial purification of bacteriophage M13 using polymer-salt and ionic liquid-salt ATPS. From such study, it was concluded that PEG 400-potassium phosphate ATPS with a volume ratio (VR ) of 1 and 25% w/w TLL were the best suitable for the primary recovery of bacteriophage M13 from a crude extract, achieving a recovery yield of 83...
June 8, 2018: Biotechnology Progress
Martin Kögler, Andrea Paul, Emmanuel Anane, Mario Birkholz, Alex Bunker, Tapani Viitala, Michael Maiwald, Stefan Junne, Peter Neubauer
The application of Raman spectroscopy as a monitoring technique for bioprocesses is severely limited by a large background signal originating from fluorescing compounds in the culture media. Here we compare time-gated Raman (TG-Raman)-, continuous wave NIR-process Raman (NIR-Raman) and continuous wave micro-Raman (micro-Raman) approaches in combination with surface enhanced Raman spectroscopy (SERS) for their potential to overcome this limit. For that purpose, we monitored metabolite concentrations of Escherichia coli bioreactor cultivations in cell-free supernatant samples...
June 8, 2018: Biotechnology Progress
Ornella M Ontañon, Silvina Ghio, Rubén Marrero Díaz de Villegas, Florencia E Piccinni, Paola M Talia, María L Cerutti, Eleonora Campos
Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires multiple enzymes acting concertedly. GH43 β-xylosidases are among the most interesting enzymes involved in hemicellulose deconstruction into xylose. In this work, the structural and functional properties of β-xylosidase EcXyl43 from Enterobacter sp. were thoroughly characterized. Molecular modeling suggested a 3D structure formed by a conserved N-terminal catalytic domain linked to an ancillary C-terminal domain...
June 6, 2018: Applied Microbiology and Biotechnology
Peter Satzer, Ralf Sommer, Paulsson Johanna, Agnes Rodler, Romana Zehetner, Klaus Hofstädter, Christoph Klade, Alois Jungbauer
We developed a novel analytical method for concentration determination of tandem single-chain antibody diphtheria toxin (immunotoxin). The method is based on polymethacrylate monoliths with Protein L ligands as the binding moiety. Different buffers were tested for elution of the Protein L bound immunotoxin and 4.5 M guanidinium hydrochloride performed best. We optimized the elution conditions and the method sequence resulting in a fast and robust method with a runtime below 10 min. Fast determination of immunotoxin is critical if any process decisions rely on this data...
June 6, 2018: Journal of Separation Science
Natalia L Rojas, Elizabeth S Lewkowicz, Matias L Nobile
An industrial-scale, profitable method for production of the most widely used bioinsecticide, Bacillus thuringiensis (Bt), is challenging because of its widespread application. The aim of this study is to present a strategy to develop a low-cost, large-scale bioprocess to produce Bt H14. This study was first focused on the design of a culture medium composed of economical and available components, such as glycerol and lysed Saccharomyces cerevisiae. The production goal of 1200 ITU was achieved using a medium composed of 20:20 g L-1 of glycerol:lysed yeast in batch cultures...
June 5, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Xiao-Ling Tang, Xiao Liu, Hui Suo, Zhi-Chao Wang, Ren-Chao Zheng, Yu-Guo Zheng
The tyrosine phenol lyase (TPL) catalyzed synthesis of L-DOPA was regarded as one of the most economic route for L-DOPA synthesis. In our previous study, a novel TPL from Fusobacterium nucleatum (Fn-TPL) was exploited for efficient biosynthesis of L-DOPA. However, the catalytic efficiency decreased when the reaction system expanded from 100 mL to 1 L. As such, the bioprocess for scale-up production of L-DOPA was developed in this study. To increase the stability of substrate and product, as well as decrease the by-product formation, the optimum temperature and pH were determined to be 15 °C and pH 8...
June 5, 2018: Bioprocess and Biosystems Engineering
Lorena Fernández-Cabezón, Beatriz Galán, José L García
Nowadays steroid manufacturing occupies a prominent place in the pharmaceutical industry with an annual global market over $10 billion. The synthesis of steroidal active pharmaceutical ingredients (APIs) such as sex hormones (estrogens, androgens, and progestogens) and corticosteroids is currently performed by a combination of microbiological and chemical processes. Several mycobacterial strains capable of naturally metabolizing sterols (e.g., cholesterol, phytosterols) are used as biocatalysts to transform phytosterols into steroidal intermediates (synthons), which are subsequently used as key precursors to produce steroidal APIs in chemical processes...
2018: Frontiers in Microbiology
Yonatan Y Lipsitz, Curtis Woodford, Ting Yin, Jacob H Hanna, Peter W Zandstra
The development of cell-based therapies to replace missing or damaged tissues within the body or generate cells with a unique biological activity requires a reliable and accessible source of cells. Human pluripotent stem cells (hPSC) have emerged as a strong candidate cell source capable of extended propagation in vitro and differentiation to clinically relevant cell types. However, the application of hPSC in cell-based therapies requires overcoming yield limitations in large-scale hPSC manufacturing. We explored methods to convert hPSC to alternative states of pluripotency with advantageous bioprocessing properties, identifying a suspension-based small-molecule and cytokine combination that supports increased single-cell survival efficiency, faster growth rates, higher densities, and greater expansion than control hPSC cultures...
June 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
Inês P Rosinha Grundtvig, Søren Heintz, Ulrich Krühne, Krist V Gernaey, Patrick Adlercreutz, John D Hayler, Andy S Wells, John M Woodley
The application of conventional organic solvents has been essential in several steps of bioprocesses in order to achieve sufficient economic efficiency. The use of organic solvents is frequently essential either to partly or fully replace water in the reaction medium or as a process aid for downstream separation. Nowadays, manufacturers are increasingly requested to avoid and substitute solvents with hazardous potential. Therefore, the solvent selection must account for potential environmental hazards, health and safety problems, in addition to fulfilling the ideal characteristics for application in a process...
June 1, 2018: Biotechnology Advances
Alexandra Hofer, Christoph Herwig, Oliver Spadiut
Complex raw materials are widely used as supplements in biopharmaceutical production processes due to their positive effect on biomass growth and productivity at low cost. However, their use negatively impacts process reproducibility due to high lot-to-lot variability which contradicts current regulatory guidelines. In this study we investigated crude soy bean oil (SBO) which is a common complex raw material for filamentous fungi. We demonstrated that lecithin, which we define as phosphatidylcholines, is in fact the key material attribute in crude SBO positively affecting fungal growth and consequently productivity...
June 1, 2018: AMB Express
A B M Sharif Hossain, Musamma M Uddin, Mohammad Fawzi, Vajid N Veettil
The nano-cellulose derived nano-biofilm keeps a magnificent role in medical, biomedical, bioengineering and pharmaceutical industries. Plant biomaterial is naturally organic and biodegradable. This study has been highlighted as one of the strategy introducing biomass based nano-bioplastic (nanobiofilm) to solve dependency on petroleum and environment pollution because of non-degradable plastic. The data study was carried out to investigate the nano-biopolymer (nanocellulose) based nano-biofilm data from corn leaf biomass coming after bioprocess technology without chemicals...
April 2018: Data in Brief
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