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Yağmur Koskun, Aysun Şavk, Betül Şen, Fatih Şen
Glucose enzyme biosensors have been used for a variety of applications such as medical diagnosis, bioprocess engineering, beverage industry and environmental scanning etc. and there is still a growing interest in glucose sensors. For this purpose, addressed herein, as a novel glucose sensor, highly sensitive activated carbon (AC) decorated monodisperse nickel and palladium alloy nanocomposites modified glassy carbon electrode (Ni-Pd@AC/GCE NCs) have been synthesized by in-situ reduction technique. Raman Spectroscopy (RS), X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), cyclic voltammetry (CV) and chronoamperometry (CA) were used for the characterization of the prepared non-enzymatic glucose sensor...
June 20, 2018: Analytica Chimica Acta
Kejing Zhang, Mengying Si, Dan Liu, Shengnan Zhuo, Mingren Liu, Hui Liu, Xu Yan, Yan Shi
Background: The recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strain Cupriavidus basilensis B-8 (here after B-8) to pretreat the rice straw (RS) by mimicking the natural fungal invasion process. Here, we also elaborated the mechanism through conducting a systematic study of physicochemical changes before and after pretreatment...
2018: Biotechnology for Biofuels
Vo Van Giau, Hyon Lee, Kyu Hwan Shim, Eva Bagyinszky, Seong Soo A An
Genetic variations play an important role in the clinical presentation and progression of Alzheimer's disease (AD), especially early-onset Alzheimer's disease. Hundreds of mutations have been reported with the majority resulting from alterations in β-amyloid precursor protein ( APP ), presenilin 1 ( PSEN1 ), or presenilin 2 ( PSEN2 ) genes. The roles of these mutations in the pathogenesis of AD have been classically confirmed or refuted through functional studies, where the mutations are cloned, inserted into cell lines, and monitored for changes in various properties including cell survival, amyloid production, or Aβ42/40 ratio...
2018: Clinical Interventions in Aging
Priscila Marques da Paz, Juliana de Oliveira
This work is based on the importance of monitoring the thermodynamic variables of sugarcane juice fermentation by Saccharomyces cerevisiae, using a numerical technique, and providing artifices that lead to the best performance of this bioprocess. Different combinations of yeast quantity were added to diverse dilutions of cane juice, allowing the evaluation of the fermentation performance. This was conducted by observing the temperature signal obtained from thermal probes inserted in the experimental set up...
February 13, 2018: Bioprocess and Biosystems Engineering
Frederico Mendonça Bahia, Gabriela Carneiro de Almeida, Lorena Pereira de Andrade, Christiane Gonçalves Campos, Lúcio Rezende Queiroz, Rayane Luzia Vieira da Silva, Patrícia Verardi Abdelnur, José Raimundo Corrêa, Maurizio Bettiga, Nádia Skorupa Parachin
Biosurfactants are biological tensioactive agents that can be used in the cosmetic and food industries. Rhamnolipids are glycolipid biosurfactants naturally produced by Pseudomonas aeruginosa and are composed of one or two rhamnose molecules linked to beta-hydroxy fatty acid chains. These compounds are green alternatives to petrochemical surfactants, but their large-scale production is still in its infancy, hindered due to pathogenicity of natural producer, high substrate and purification costs and low yields and productivities...
February 13, 2018: Scientific Reports
Joo Shun Tan, Sahar Abbasiliasi, Saeid Kadkhodaei, Yew Joon Tam, Teck-Kim Tang, Yee-Ying Lee, Arbakariya B Ariff
BACKGROUND: Demand for high-throughput bioprocessing has dramatically increased especially in the biopharmaceutical industry because the technologies are of vital importance to process optimization and media development. This can be efficiently boosted by using microtiter plate (MTP) cultivation setup embedded into an automated liquid-handling system. The objective of this study was to establish an automated microscale method for upstream and downstream bioprocessing of α-IFN2b production by recombinant Escherichia coli...
January 4, 2018: BMC Microbiology
Roman Brunecky, Daehwan Chung, Nicholas S Sarai, Neal Hengge, Jordan F Russell, Jenna Young, Ashutosh Mittal, Patthra Pason, Todd Vander Wall, William Michener, Todd Shollenberger, Janet Westpheling, Michael E Himmel, Yannick J Bomble
Background: Thermophilic microorganisms and their enzymes offer several advantages for industrial application over their mesophilic counterparts. For example, a hyperthermophilic anaerobe, Caldicellulosiruptor bescii, was recently isolated from hot springs in Kamchatka, Siberia, and shown to have very high cellulolytic activity. Additionally, it is one of a few microorganisms being considered as viable candidates for consolidated bioprocessing applications. Moreover, C. bescii is capable of deconstructing plant biomass without enzymatic or chemical pretreatment...
2018: Biotechnology for Biofuels
Yu Zheng, Yangang Chang, Sankuan Xie, Jia Song, Min Wang
Aerobic Acetobacter pasteurianus is one of the most widely used bacterial species for acetic acid and vinegar production. The acetic acid condition is the primary challenge to the industrial application of A. pasteurianus. Thus, numerous endeavors, including strain improvement and process control, have been performed to improve the product formation and acetic acid tolerance of A. pasteurianus. The metabolic features of A. pasteurianus have been gradually elucidated through omic techniques, such as genomics and proteomics...
February 11, 2018: Applied Microbiology and Biotechnology
Mohammad Safarpoor, Mehrorang Ghaedi, Arash Asfaram, Masoumeh Yousefi-Nejad, Hamedreza Javadian, Hossein Zare Khafri, Marzieh Bagherinasab
The present study is devoted to prepare a new antibacterial and antifungal agent based on in situ-synthesized silver nanoparticles at room temperature using Rosmarinus officinalis (R. officinalis) leaf extract. The Ag-NPs characterization by UV-visible, SEM, TEM and XRD revealed that the particles sizes were in the range of 10-33 nm. In this study, hydroalcoholic extracts were used with ultrasonic method. Ultrasonication has recently received attention as a novel bioprocessing tool for process intensification in many areas of downstream processing...
April 2018: Ultrasonics Sonochemistry
Simone Albrecht, Christian Kaisermayer, Clair Gallagher, Amy Farrell, Anna Lindeberg, Jonathan Bones
Cell viability has a critical impact on product quantity and quality during the biomanufacturing of therapeutic proteins. An advanced understanding of changes in the cellular and conditioned media proteomes upon cell stress and death is therefore needed for improved bioprocess control. Here, a high pH / low pH reversed phase data independent 2D-LC-MSE discovery proteomics platform was applied to study the cellular and conditioned media proteomes of CHO-K1 apoptosis and necrosis models where cell death was induced by staurosporine exposure or aeration shear in a benchtop bioreactor, respectively...
February 10, 2018: Biotechnology and Bioengineering
Seyed Mohammad Bagher Hashemi, Amin Mousavi Khaneghah, Jorge A Saraiva, Anet Režek Jambrak, Francisco J Barba, Maria J Mota
Date syrup is rich in fermentable sugars and may be used as a substrate for different microbial fermentations, including lactic acid fermentation processes. The beneficial effects of ultrasounds (US) on bioprocesses have been reported for several microorganisms, due to the enhancement of cell growth, as well as improvements in yields and productivities. Therefore, US treatments (30 kHz, 100 W, 10-30 min) were applied to two lactobacilli (Lactobacillus helveticus PTCC 1332 and Lactobacillus acidophilus PTCC 1643), during fermentation using date syrup as substrate...
February 9, 2018: Applied Microbiology and Biotechnology
Xue Chi, Jianzheng Li, Xin Wang, Yafei Zhang, Philip Antwi
A novel consolidated bioprocess for hyper-production of butyric acid from delignified rice straw without exogenous enzymes involved was developed by co-fermentation of Clostridium thermocellum ATCC 27405 and C. thermobutyricum ATCC 49875. Feasibility of the consolidated bioprocess was approved by batch fermentations, with the optimum pH of 6.5. Fed-batch fermentation with a constant pH of 6.5 at 55 °C could enhance the butyric acid yield to a remarkable 33.9 g/L with a selectivity as high as 78%. Metabolic analysis of the co-culture indicated that sugars liberated by C...
January 9, 2018: Bioresource Technology
Jia Wang, Mohit Bibra, Kasthuri Venkateswaran, David R Salem, Navanietha Krishnaraj Rathinam, Venkataraman Gadhamshetty, Rajesh K Sani
Human waste simulants were for the first time converted into biohydrogen by a newly developed anaerobic microbial consortium via thermophilic consolidated bioprocessing. Four different BioH2-producing consortia (denoted as C1, C2, C3 and C4) were isolated, and developed using human waste simulants as substrate. The thermophilic consortium C3, which contained Thermoanaerobacterium, Caloribacterium, and Caldanaerobius species as the main constituents, showed the highest BioH2 production (3.999 mmol/g) from human waste simulants under optimized conditions (pH 7...
February 3, 2018: Bioresource Technology
Sidharth Arora, Richa Rani, Sanjoy Ghosh
In recent years, substantial credibility in employing Solid-State Fermentation (SSF) technique has been witnessed owing to its numerous advantages over submerged fermentation (SmF). In spite of enormous advantages, true potential of SSF technology has not been fully realized at industrial scale. The lack of rational and scalable bioreactor designs backed by mathematical models and automated control system that could successfully address heterogeneity with respect to heat and mass, and also operate aseptically, remains the prime reason for it...
February 1, 2018: Journal of Biotechnology
Sang-Min Paik, EonSeon Jin, Sang Jun Sim, Noo Li Jeon
Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10-30 Hz and 0.15-0.45 G) was used to prime the cells for enhanced biomass. When exposed to 10 Hz at 0.15 G for 72 h and inoculated in baffled flasks of large shear fields (0...
January 31, 2018: Bioresource Technology
Sara Pereira, Helene F Kildegaard, Mikael R Andersen
For over three decades, Chinese hamster ovary (CHO) cells have been the chosen expression platform for the production of therapeutic proteins with complex post-translational modifications. However, the metabolism of these cells is far from perfect and optimized, and requires substantial knowhow and process optimization and monitoring to perform efficiently. One of the main reasons for this is the production and accumulation of toxic and growth-inhibiting metabolites during culture. Lactate and ammonium are the most known, but many more have been identified...
February 2, 2018: Biotechnology Journal
Yayun Wang, Kei Okugawa, Emi Kunitake, Makiko Sakka, Tetsuya Kimura, Kazuo Sakka
Although Ruminiclostridium josui (formerly Clostridium josui), a strictly anaerobic mesophilic cellulolytic bacterium, is a promising candidate for biomass utilization via consolidated bioprocessing, its host-vector system has not yet been established. The existence of a restriction and modification system is a significant barrier to the transformation of R. josui. Here, we partially purified restriction endonuclease RjoI from R. josui cell extract using column chromatography. Further characterization showed that RjoI is an isoschizomer of DpnI, recognizing the sequence 5'-Gmet ATC-3', where the A nucleotide is Dam-methylated...
February 1, 2018: Journal of Basic Microbiology
Tooru Ooya, Takaya Ogawa, Toshifumi Takeuchi
Enzyme application has gained importance over the past decade in bioprocess, biomedical, and pharmaceutical fields. We found that polyglycerol dendrimers (PGDs), which are biocompatible molecules, can recover alcohol dehydrogenase (ADH) from aqueous solution under elevated temperature. A low concentration of PGD (5 wt.%) is sufficient for the recovery of high enzymatic activity, although a high concentration (25-75 wt.%) of glycerol is generally required to stabilize ADH. The enzymatic activity of ADH in suspension with PGDs is over 60% but it is only 10% in that with glycerol...
February 1, 2018: Journal of Biomaterials Science. Polymer Edition
Shu-Ping Zou, Kai Gu, Yu-Guo Zheng
Halohydrin dehalogenase (HHDH)-mediated dehalogenation of 1,3-dichloro-2-propanol (1,3-DCP) is a key step in the chemoenzymatic synthesis of epichlorohydrin (ECH) from glycerol. In this study, a covalent immobilization strategy was employed to enhance the stability of Agrobacterium tumefaciens HHDH by using epoxy resin ES-103B as a carrier. Under optimal conditions, the activity recovery of ES-103B-immobilized HHDH (HHDH@ES-103B) was 62.4% and the specific activity was 1604 U/g. The HHDH@ES-103B exhibited excellent thermostability, with a half-life of 68...
February 1, 2018: Biotechnology Progress
Ali Nikdel, Richard D Braatz, Hector M Budman
Dynamic flux balance analysis (DFBA) has become an instrumental modeling tool for describing the dynamic behavior of bioprocesses. DFBA involves the maximization of a biologically meaningful objective subject to kinetic constraints on the rate of consumption/production of metabolites. In this paper, we propose a systematic data-based approach for finding both the biological objective function and a minimum set of active constraints necessary for matching the model predictions to the experimental data. The proposed algorithm accounts for the errors in the experiments and eliminates the need for ad hoc choices of objective function and constraints as done in previous studies...
February 1, 2018: Bioprocess and Biosystems Engineering
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