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Bioprocessing

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https://www.readbyqxmd.com/read/28078400/characterization-of-four-endophytic-fungi-as-potential-consolidated-bioprocessing-hosts-for-conversion-of-lignocellulose-into-advanced-biofuels
#1
Weihua Wu, Ryan W Davis, Mary Bao Tran-Gyamfi, Alan Kuo, Kurt LaButti, Sirma Mihaltcheva, Hope Hundley, Mansi Chovatia, Erika Lindquist, Kerrie Barry, Igor V Grigoriev, Bernard Henrissat, John M Gladden
Recently, several endophytic fungi have been demonstrated to produce volatile organic compounds (VOCs) with properties similar to fossil fuels, called "mycodiesel," while growing on lignocellulosic plant and agricultural residues. The fact that endophytes are plant symbionts suggests that some may be able to produce lignocellulolytic enzymes, making them capable of both deconstructing lignocellulose and converting it into mycodiesel, two properties that indicate that these strains may be useful consolidated bioprocessing (CBP) hosts for the biofuel production...
January 12, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28063758/acidic-ph-with-coordinated-reduction-of-basic-fibroblast-growth-factor-maintains-the-glioblastoma-stem-cell-like-phenotype-in%C3%A2-vitro
#2
Elizabeth M Haley, Samantha G Tilson, Ursula L Triantafillu, Justin W Magrath, Yonghyun Kim
Glioblastoma stem cells (GSCs) are a unique subpopulation of cells within glioblastoma multiforme (GBM) brain tumors that possess the ability to self-renew and differentiate into bulk tumor cells. GSCs are resistant to currently available treatments and are the likely culprit behind tumor relapse in GBM patients. However, GSCs are currently inaccessible to the larger scientific community because obtaining a sufficient number of GSCs remains technically challenging and cost-prohibitive. Thus, the objective of this study was to develop a more efficient GSC culture strategy that results in a higher cell yield of GSCs at a lower cost...
January 4, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28059717/industrial-biomanufacturing-the-future-of-chemical-production
#3
REVIEW
James M Clomburg, Anna M Crumbley, Ramon Gonzalez
The current model for industrial chemical manufacturing employs large-scale megafacilities that benefit from economies of unit scale. However, this strategy faces environmental, geographical, political, and economic challenges associated with energy and manufacturing demands. We review how exploiting biological processes for manufacturing (i.e., industrial biomanufacturing) addresses these concerns while also supporting and benefiting from economies of unit number. Key to this approach is the inherent small scale and capital efficiency of bioprocesses and the ability of engineered biocatalysts to produce designer products at high carbon and energy efficiency with adjustable output, at high selectivity, and under mild process conditions...
January 6, 2017: Science
https://www.readbyqxmd.com/read/28057917/an-integrated-miniature-bioprocessing-for-personalized-human-induced-pluripotent-stem-cell-expansion-and-differentiation-into-neural-stem-cells
#4
Haishuang Lin, Qiang Li, Yuguo Lei
Human induced pluripotent stem cells (iPSCs) are ideal cell sources for personalized cell therapies since they can be expanded to generate large numbers of cells and differentiated into presumably all the cell types of the human body in vitro. In addition, patient specific iPSC-derived cells induce minimal or no immune response in vivo. However, with current cell culture technologies and bioprocessing, the cost for biomanufacturing clinical-grade patient specific iPSCs and their derivatives are very high and not affordable for majority of patients...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28053663/utilization-of-xylose-by-engineered-strains-of-ashbya-gossypii-for-the-production-of-microbial-oils
#5
David Díaz-Fernández, Patricia Lozano-Martínez, Rubén M Buey, José Luis Revuelta, Alberto Jiménez
BACKGROUND: Ashbya gossypii is a filamentous fungus that is currently exploited for the industrial production of riboflavin. The utilization of A. gossypii as a microbial biocatalyst is further supported by its ability to grow in low-cost feedstocks, inexpensive downstream processing and the availability of an ease to use molecular toolbox for genetic and genomic modifications. Consequently, A. gossypii has been also introduced as an ideal biotechnological chassis for the production of inosine, folic acid, and microbial oils...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28052085/exploiting-bioprocessing-fluctuations-to-elicit-the-mechanistics-of-de-novo-lipogenesis-in-yarrowia-lipolytica
#6
Andreas E Vasdekis, Andrew M Silverman, Gregory Stephanopoulos
Despite substantial achievements in elucidating the metabolic pathways of lipogenesis, a mechanistic representation of lipid accumulation and degradation has not been fully attained to-date. Recent evidence suggests that lipid accumulation can occur through increases of either the cytosolic copy-number of lipid droplets (LDs), or the LDs size. However, the prevailing phenotype, or how such mechanisms pertain to lipid degradation remain poorly understood. To address this shortcoming, we employed the-recently discovered-innate bioprocessing fluctuations in Yarrowia lipolytica, and performed single-cell fluctuation analysis using optical microscopy and microfluidics that generate a quasi-time invariant microenvironment...
2017: PloS One
https://www.readbyqxmd.com/read/28045110/simplifying-multidimensional-fermentation-dataset-analysis-and-visualization-one-step-closer-to-capturing-high-quality-mutant-strains
#7
Xiang Zhou, Dan Xu, Ting-Ting Jiang
In this study, we analyzed mutants of Clostridium acetobutylicum, an organism used in a broad range of industrial processes related to biofuel production, to facilitate future studies of bioreactor and bioprocess design and scale-up, which are very important research projects for industrial microbiology applications. To accomplish this, we generated 329 mutant strains and applied principal component analysis (PCA) to fermentation data gathered from these strains to identify a core set of independent features for comparison...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28019710/mammalian-cell-culture-monitoring-using-in-situ-spectroscopy-is-your-method-really-optimised
#8
Silvère André, Sylvain Lagresle, Zahia Hannas, Éric Calvosa, Ludovic Duponchel
In recent years, as a result of the process analytical technology (PAT) initiative of the US Food and Drug Administration (FDA), many different works have been carried out on direct and in situ monitoring of critical parameters for mammalian cell cultures by Raman spectroscopy and multivariate regression techniques. However, despite interesting results, it cannot be said that the proposed monitoring strategies, which will reduce errors of the regression models and thus confidence limits of the predictions, are really optimized...
December 26, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/28019665/the-impact-of-left-ventricular-stretching-in-model-cultivations-with-neonatal-cardiomyocytes-in-a-whole-heart-bioreactor
#9
Jörn Hülsmann, Hug Aubin, Alexander Wehrmann, Artur Lichtenberg, Payam Akhyari
Here, we investigate the impact of integrated three-dimensional (3D) left ventricular (LV) stretching on myocardial maturation in a whole-heart bioreactor setting. Therefore, decellularized rat hearts were selectively repopulated with rodent neonatal cardiomyocytes (5•10(6) cells per heart) and cultured over 5 d. Continuous medium perfusion was maintained through the coronary artery system in a customized whole-heart bioreactor system with or without integrated biomechanical stimulation of LV. 3D repopulation effectiveness and cellular vitality were evaluated by repetitive metabolic WST-1 assays and 3D confocal microscopy analysis through fluorescent staining, also assessing cellular organization...
December 26, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28017614/fto-plays-an-oncogenic-role-in-acute-myeloid-leukemia-as-a-n-6-methyladenosine-rna-demethylase
#10
Zejuan Li, Hengyou Weng, Rui Su, Xiaocheng Weng, Zhixiang Zuo, Chenying Li, Huilin Huang, Sigrid Nachtergaele, Lei Dong, Chao Hu, Xi Qin, Lichun Tang, Yungui Wang, Gia-Ming Hong, Hao Huang, Xiao Wang, Ping Chen, Sandeep Gurbuxani, Stephen Arnovitz, Yuanyuan Li, Shenglai Li, Jennifer Strong, Mary Beth Neilly, Richard A Larson, Xi Jiang, Pumin Zhang, Jie Jin, Chuan He, Jianjun Chen
N(6)-Methyladenosine (m(6)A) represents the most prevalent internal modification in mammalian mRNAs. Despite its functional importance in various fundamental bioprocesses, the studies of m(6)A in cancer have been limited. Here we show that FTO, as an m(6)A demethylase, plays a critical oncogenic role in acute myeloid leukemia (AML). FTO is highly expressed in AMLs with t(11q23)/MLL rearrangements, t(15;17)/PML-RARA, FLT3-ITD, and/or NPM1 mutations. FTO enhances leukemic oncogene-mediated cell transformation and leukemogenesis, and inhibits all-trans-retinoic acid (ATRA)-induced AML cell differentiation, through regulating expression of targets such as ASB2 and RARA by reducing m(6)A levels in these mRNA transcripts...
January 9, 2017: Cancer Cell
https://www.readbyqxmd.com/read/28013134/production-of-a-generic-microbial-feedstock-for-lignocellulose-biorefineries-through-sequential-bioprocessing
#11
Chen-Wei Chang, Colin Webb
Lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. Reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. In this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. In this research, the utilisation of solid-state bioprocessing using the fungus Trichoderma longibrachiatum was implemented as a simultaneous microbial pretreatment and in-situ enzyme production method for fungal autolysis and further enzyme hydrolysis of fermented solids...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28012009/hydrolase-bioh-knockout-in-e-coli-enables-efficient-fatty-acid-methyl-ester-bioprocessing
#12
Marvin Kadisch, Andreas Schmid, Bruno Bühler
Fatty acid methyl esters (FAMEs) originating from plant oils are most interesting renewable feedstocks for biofuels and bio-based materials. FAMEs can also be produced and/or functionalized by engineered microbes to give access to, e.g., polymer building blocks. Yet, they are often subject to hydrolysis yielding free fatty acids, which typically are degraded by microbes. We identified BioH as the key enzyme responsible for the hydrolysis of medium-chain length FAME derivatives in different E. coli K-12 strains...
December 24, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28005282/paradigm-shift-in-virus-like-particle-bioprocessing
#13
(no author information available yet)
No abstract text is available yet for this article.
February 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27999966/consolidated-ethanol-production-from-jerusalem-artichoke-tubers-at-elevated-temperature-by-saccharomyces-cerevisiae-engineered-with-inulinase-expression-through-cell-surface-display
#14
M Mahfuza Khatun, Chen-Guang Liu, Xin-Qing Zhao, Wen-Jie Yuan, Feng-Wu Bai
Ethanol fermentation from Jerusalem artichoke tubers was performed at elevated temperatures by the consolidated bioprocessing strategy using Saccharomyces cerevisiae MK01 expressing inulinase through cell surface display. No significant difference was observed in yeast growth when temperature was controlled at 38 and 40 °C, respectively, but inulinase activity with yeast cells was substantially enhanced at 40 °C. As a result, enzymatic hydrolysis of inulin was facilitated and ethanol production was improved with 89...
December 20, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27997085/effect-of-guanidine-and-arginine-on-protein-ligand-interactions-in-multimodal-cation-exchange-chromatography
#15
Siddharth Parimal, Shekhar Garde, Steven M Cramer
The addition of fluid phase modifiers provides significant opportunities for increasing the selectivity of multimodal chromatography. In order to optimize this selectivity, it is important to understand the fundamental interactions between proteins and these modifiers. To this end, Molecular Dynamics (MD) simulations were first performed to study the interactions of guanidine and arginine with three proteins. The simulation results showed that both guanidine and arginine interacted primarily with the negatively charged regions on the proteins and that these regions could be readily predicted using electrostatic potential maps...
December 20, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27994508/crispr-cas9-from-genome-editing-to-cancer-research
#16
REVIEW
Si Chen, Heng Sun, Kai Miao, Chu-Xia Deng
Cancer development is a multistep process triggered by innate and acquired mutations, which cause the functional abnormality and determine the initiation and progression of tumorigenesis. Gene editing is a widely used engineering tool for generating mutations that enhance tumorigenesis. The recent developed clustered regularly interspaced short palindromic repeats-CRISPR-associated 9 (CRISPR-Cas9) system renews the genome editing approach into a more convenient and efficient way. By rapidly introducing genetic modifications in cell lines, organs and animals, CRISPR-Cas9 system extends the gene editing into whole genome screening, both in loss-of-function and gain-of-function manners...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27991569/identification-and-characterization-of-roseltide-a-knottin-type-neutrophil-elastase-inhibitor-derived-from-hibiscus-sabdariffa
#17
Shining Loo, Antony Kam, Tianshu Xiao, Giang K T Nguyen, Chuan Fa Liu, James P Tam
Plant knottins are of therapeutic interest due to their high metabolic stability and inhibitory activity against proteinases involved in human diseases. The only knottin-type proteinase inhibitor against porcine pancreatic elastase was first identified from the squash family in 1989. Here, we report the identification and characterization of a knottin-type human neutrophil elastase inhibitor from Hibiscus sabdariffa of the Malvaceae family. Combining proteomic and transcriptomic methods, we identified a panel of novel cysteine-rich peptides, roseltides (rT1-rT8), which range from 27 to 39 residues with six conserved cysteine residues...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27977908/integrated-continuous-processing-of-proteins-expressed-as-inclusion-bodies-gcsf-as-a-case-study
#18
Nikhil Kateja, Harshit Agarwal, Vishwanath Hebbi, Anurag S Rathore
Affordability of biopharmaceuticals continues to be a challenge, particularly in developing economies. This has fuelled advancements in manufacturing that can offer higher productivity and better economics without sacrificing product quality in the form of an integrated continuous manufacturing platform. While platform processes for monoclonal antibodies have existed for more than a decade, development of an integrated continuous manufacturing process for bacterial proteins has received relatively scant attention...
December 15, 2016: Biotechnology Progress
https://www.readbyqxmd.com/read/27975588/hptlc-and-hptlc-coupled-bioassays-for-bioprocess-control-of-medicinal-plant-in-vitro-cultures-from-the-balkan-region
#19
E Wolfram, S Bräm, M Todorova, A Trendafilova, A Alvarez, D Ortega, P Koleva, K Danova
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27974629/throughput-optimization-of-continuous-biopharmaceutical-manufacturing-facilities
#20
Fernando Antonio Garcia, Michael W Vandiver
In order to operate profitably under different product demand scenarios, biopharmaceutical companies must design their facilities with mass output flexibility in mind. Traditional biologics manufacturing technologies pose operational challenges in this regard due to their high costs and slow equipment turnaround times, restricting the types of products and mass quantities that can be processed. Modern plant design, however, has facilitated the development of lean and efficient bioprocessing facilities through footprint reduction, and adoption of disposable and continuous manufacturing technologies...
December 14, 2016: PDA Journal of Pharmaceutical Science and Technology
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