keyword
MENU ▼
Read by QxMD icon Read
search

Bioprocessing

keyword
https://www.readbyqxmd.com/read/29689438/simultaneous-saccharification-and-bioethanol-production-from-corn-cobs-process-optimization-and-kinetic-studies
#1
Yeshona Sewsynker-Sukai, E B Gueguim Kana
This study investigates the simultaneous saccharification and fermentation (SSF) process for bioethanol production from corn cobs with prehydrolysis (PSSF) and without prehydrolysis (OSSF). Two response surface models were developed with high coefficients of determination (>0.90). Process optimization gave high bioethanol concentrations and bioethanol conversions for the PSSF (36.92 ± 1.34 g/L and 62.36 ± 2.27%) and OSSF (35.04 ± 0.170 g/L and 58.13 ± 0.283%) models respectively. Additionally, the logistic and modified Gompertz models were used to study the kinetics of microbial cell growth and ethanol formation under microaerophilic and anaerobic conditions...
April 17, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29686311/rna-n-6-methyladenosine-modification-in-cancers-current-status-and-perspectives
#2
Xiaolan Deng, Rui Su, Hengyou Weng, Huilin Huang, Zejuan Li, Jianjun Chen
N6 -methyladenosine (m6 A), the most abundant internal modification in eukaryotic messenger RNAs (mRNAs), has been shown to play critical roles in various normal bioprocesses such as tissue development, stem cell self-renewal and differentiation, heat shock or DNA damage response, and maternal-to-zygotic transition. The m6 A modification is deposited by the m6 A methyltransferase complex (MTC; i.e., writer) composed of METTL3, METTL14 and WTAP, and probably also VIRMA and RBM15, and can be removed by m6 A demethylases (i...
April 23, 2018: Cell Research
https://www.readbyqxmd.com/read/29685337/evaluation-of-recent-protein-a-stationary-phase-innovations-for-capture-of-biotherapeutics
#3
Timothy M Pabst, Johnny Thai, Alan K Hunter
We describe a comprehensive evaluation of 12 Protein A stationary phases for capture of biotherapeutics. We first examine the morphological properties of the stationary phases using a variety of orthogonal techniques including electron microscopy, particle sizing, pressure-flow behavior, and isocratic pulse response. A panel of nine proteins spanning a wide range of structures and biochemical properties was then used to assess equilibrium uptake, mass transport, dynamic binding capacity, and elution pH. Process performance and product quality were also examined under realistic bioprocess conditions using clarified mammalian cell culture broth...
April 7, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29680868/an-integrated-bio-process-for-production-of-functional-biomolecules-utilizing-raw-and-by-products-from-dairy-and-sugarcane-industries
#4
Kusum Lata, Manisha Sharma, Satya Narayan Patel, Rajender S Sangwan, Sudhir P Singh
The study investigated an integrated bioprocessing of raw and by-products from sugarcane and dairy industries for production of non-digestible prebiotic and functional ingredients. The low-priced feedstock, whey, molasses, table sugar, jaggery, etc., were subjected to transglucosylation reactions catalyzed by dextransucrase from Leuconostoc mesenteroides MTCC 10508. HPLC analysis approximated production of about 11-14 g L-1 trisaccharide i.e. 2-α-D-glucopyranosyl-lactose (4-galactosyl-kojibiose) from the feedstock prepared from table sugar, jaggery, cane molasses and liquid whey, containing about 30 g L-1 sucrose and lactose each...
April 21, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29679131/process-optimization-involving-critical-evaluation-of-oxygen-transfer-oxygen-uptake-and-nitrogen-limitation-for-enhanced-biomass-and-lipid-production-by-oleaginous-yeast-for-biofuel-application
#5
Jayita Chopra, Ramkrishna Sen
Lipid accumulation in oleaginous yeast is generally induced by nitrogen starvation, while oxygen saturation can influence biomass growth. Systematic shake flask studies that help in identifying the right nitrogen source and relate its uptake kinetics to lipid biosynthesis under varying oxygen saturation conditions are very essential for addressing the bioprocessing-related issues, which are envisaged to occur in the fermenter scale production. In the present study, lipid bioaccumulation by P. guilliermondii at varying C:N ratios and oxygen transfer conditions (assessed in terms of kL a) was investigated in shake flasks using a pre-optimized N-source and a two-stage inoculum formulated in a hybrid medium...
April 20, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29674565/systematic-analysis-of-complex-genetic-interactions
#6
Elena Kuzmin, Benjamin VanderSluis, Wen Wang, Guihong Tan, Raamesh Deshpande, Yiqun Chen, Matej Usaj, Attila Balint, Mojca Mattiazzi Usaj, Jolanda van Leeuwen, Elizabeth N Koch, Carles Pons, Andrius J Dagilis, Michael Pryszlak, Jason Zi Yang Wang, Julia Hanchard, Margot Riggi, Kaicong Xu, Hamed Heydari, Bryan-Joseph San Luis, Ermira Shuteriqi, Hongwei Zhu, Nydia Van Dyk, Sara Sharifpoor, Michael Costanzo, Robbie Loewith, Amy Caudy, Daniel Bolnick, Grant W Brown, Brenda J Andrews, Charles Boone, Chad L Myers
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and essential genes were hubs on the trigenic network. Despite their functional enrichment, trigenic interactions tended to link genes in distant bioprocesses and displayed a weaker magnitude than digenic interactions...
April 20, 2018: Science
https://www.readbyqxmd.com/read/29666707/realm-of-thermoalkaline-lipases-in-bioprocess-commodities
#7
REVIEW
Ahmad Firdaus B Lajis
For decades, microbial lipases are notably used as biocatalysts and efficiently catalyze various processes in many important industries. Biocatalysts are less corrosive to industrial equipment and due to their substrate specificity and regioselectivity they produced less harmful waste which promotes environmental sustainability. At present, thermostable and alkaline tolerant lipases have gained enormous interest as biocatalyst due to their stability and robustness under high temperature and alkaline environment operation...
2018: Journal of Lipids
https://www.readbyqxmd.com/read/29666529/natural-preservatives-for-superficial-scald-reduction-and-enhancement-of-protective-phenolic-linked-antioxidant-responses-in-apple-during-post-harvest-storage
#8
Dipayan Sarkar, Chandrakant Ankolekar, Duane Greene, Kalidas Shetty
Superficial scald during post-harvest storage is a serious problem for long-term preservation and shelf-life of some apple and pear cultivars. Development of superficial scald and related physiological disorders such as enzymatic and non-enzymatic browning are associated in part with oxidative breakdown and redox imbalance. Therefore, targeting natural antioxidants from food-grade sources as post-harvest treatment to reduce superficial scald has merit. Such natural antioxidants can potentially counter oxidation-linked damages associated with superficial scald through stimulation of antioxidant enzyme responses and biosynthesis of less-oxidized phenolics involving protective redox-linked pathway such as proline-associated pentose phosphate pathway...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29663326/contributions-of-depth-filter-components-to-protein-adsorption-in-protein-bioprocessing
#9
Ohnmar Khanal, Nripen Singh, Steven J Traylor, Xuankuo Xu, Sanchayita Ghose, Zheng Jian Li, Abraham M Lenhoff
Depth filtration is widely used in downstream bioprocessing to remove particulate contaminants via depth straining and is therefore applied to harvest clarification and other processing steps. However, depth filtration also removes proteins via adsorption, which can contribute variously to impurity clearance and to reduction in product yield. The adsorption may occur on the different components of the depth filter, i.e., filter aid, binder and cellulose filter. We measured adsorption of several model proteins and therapeutic proteins onto filter aids, cellulose and commercial depth filters at pH 5-8 and ionic strengths < 50 mM and correlated the adsorption data to bulk measured properties such as surface area, morphology, surface charge density and composition...
April 16, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29652809/characterization-of-chlorella-sorokiniana-utex-1230
#10
Alessandro Marco Lizzul, Aitor Lekuona-Amundarain, Saul Purton, Luiza Cintra Campos
This paper characterizes the strain Chlorella sorokiniana UTEX 1230 within a laboratory setting using a 1 L bubble column. The findings show that productivity can be trebled under mixotrophic conditions (from 0.2 g&middot;L&minus;1 &middot;d&minus;1 to 0.66 g&middot;L&minus;1 &middot;d&minus;1 ) with the addition of sodium acetate. The results also indicate that both the growth rate and final yield increase with the cultivation temperature, with most parameters showing an optimum in the range of 30&ndash;35 &deg;C...
April 13, 2018: Biology
https://www.readbyqxmd.com/read/29650031/the-physiological-role-of-motin-family-and-its-dysregulation-in-tumorigenesis
#11
REVIEW
Tingting Huang, Yuhang Zhou, Jinglin Zhang, Alfred S L Cheng, Jun Yu, Ka Fai To, Wei Kang
Members in Motin family, or Angiomotins (AMOTs), are adaptor proteins that localize in the membranous, cytoplasmic or nuclear fraction in a cell context-dependent manner. They control the bioprocesses such as migration, tight junction formation, cell polarity, and angiogenesis. Emerging evidences have demonstrated that AMOTs participate in cancer initiation and progression. Many of the previous studies have focused on the involvement of AMOTs in Hippo-YAP1 pathway. However, it has been controversial for years that AMOTs serve as either positive or negative growth regulators in different cancer types because of the various cellular origins...
April 12, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29644625/contributions-of-depth-filter-components-to-protein-adsorption-in-protein-bioprocessing
#12
Ohnmar Khanal, Nripen Singh, Steven J Traylor, Xuankuo Xu, Sanchayita Ghose, Zheng Jian Li, Abraham M Lenhoff
Depth filtration is widely used in downstream bioprocessing to remove particulate contaminants via depth straining and is therefore applied to harvest clarification and other processing steps. However, depth filtration also removes proteins via adsorption, which can contribute variously to impurity clearance and to reduction in product yield. The adsorption may occur on the different components of the depth filter, i.e., filter aid, binder and cellulose filter. We measured adsorption of several model proteins and therapeutic proteins onto filter aids, cellulose and commercial depth filters at pH 5-8 and ionic strengths < 50 mM and correlated the adsorption data to bulk measured properties such as surface area, morphology, surface charge density and composition...
April 12, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29644595/enhanced-bioethanol-production-from-potato-peel-waste-via-consolidated-bioprocessing-with-statistically-optimized-medium
#13
Tahmina Hossain, Abdul Bathen Miah, Siraje Arif Mahmud, Abdullah-Al- Mahin
In this study, an extensive screening was undertaken to isolate some amylolytic microorganisms capable of producing bioethanol from starchy biomass through Consolidated Bioprocessing (CBP). A total of 28 amylolytic microorganisms were isolated, from which 5 isolates were selected based on high α-amylase and glucoamylase activities and identified as Candida wangnamkhiaoensis, Hyphopichia pseudoburtonii (2 isolates), Wickerhamia sp., and Streptomyces drozdowiczii based on 26S rDNA and 16S rDNA sequencing. Wickerhamia sp...
April 12, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29644428/assessment-of-the-scalability-of-a-microtiter-plate-system-for-screening-of-oleaginous-microorganisms
#14
Gergely Kosa, Kiira S Vuoristo, Svein Jarle Horn, Boris Zimmermann, Nils Kristian Afseth, Achim Kohler, Volha Shapaval
Recent developments in molecular biology and metabolic engineering have resulted in a large increase in the number of strains that need to be tested, positioning high-throughput screening of microorganisms as an important step in bioprocess development. Scalability is crucial for performing reliable screening of microorganisms. Most of the scalability studies from microplate screening systems to controlled stirred-tank bioreactors have been performed so far with unicellular microorganisms. We have compared cultivation of industrially relevant oleaginous filamentous fungi and microalga in a Duetz-microtiter plate system to benchtop and pre-pilot bioreactors...
April 11, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29627852/bioproduction-of-2-phenylethanol-and-2-phenethyl-acetate-by-kluyveromyces-marxianus-through-the-solid-state-fermentation-of-sugarcane-bagasse
#15
Oscar Martínez, Antoni Sánchez, Xavier Font, Raquel Barrena
2-Phenylethanol (2-PE) and 2-phenethyl acetate (2-PEA) are important aroma compounds widely used in food and cosmetic industries due to their rose-like odor. Nowadays, due to the growing demand for natural products, the development of bioprocesses for obtaining value-added compounds has become of great significance. 2-PE and 2-PEA can be produced through the biotransformation of L-phenylalanine using the generally recognized as safe strain Kluyveromyces marxianus. L-phenylalanine bioconversion systems have been typically focused on submerged fermentation processes (SmF), but there is no information about other alternative productive approaches...
April 7, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29622042/engineering-crispr-interference-system-in-klebsiella-pneumoniae-for-attenuating-lactic-acid-synthesis
#16
Jingxuan Wang, Peng Zhao, Ying Li, Lida Xu, Pingfang Tian
BACKGROUND: Klebsiella pneumoniae is a promising industrial species for bioproduction of bulk chemicals such as 1,3-propanediol, 2,3-butanediol and 3-hydroxypropionic acid (3-HP). However, lactic acid is a troublesome by-product when optimizing for 3-HP production. Therefore, it is highly desirable to minimize lactic acid. RESULTS: Here, we show that lactic acid synthesis can be largely blocked by an engineered CRISPR interference (CRISPRi) system in K. pneumoniae...
April 5, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29619085/consolidated-bioprocessing-of-butanol-production-from-xylan-by-a-thermophilic-and-butanologenic-thermoanaerobacterium-sp-m5
#17
Yujia Jiang, Dong Guo, Jiasheng Lu, Peter Dürre, Weiliang Dong, Wei Yan, Wenming Zhang, Jiangfeng Ma, Min Jiang, Fengxue Xin
Background: Consolidated bioprocessing (CBP) has attracted increasing attention since it can accomplish hydrolytic enzymes production, lignocellulose degradation and microbial fermentation in one single step. Currently, biobutanol is mainly produced by mesophilic and solventogenic clostridia, such as Clostridium beijerinckii and C. acetobutylicum , which cannot directly utilize lignocellulose, an abundant, renewable and economic feedstock. Hence, metabolic construction or isolation of novel cellulolytic/hemicellulolytic and solventogenic bacteria to achieve direct butanol production from lignocellulose offers a promising alternative...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29617806/overexpression-screen-reveals-transcription-factors-involved-in-lipid-accumulation-in-yarrowia-lipolytica
#18
Christophe Leplat, Jean-Marc Nicaud, Tristan Rossignol
Yarrowia lipolytica is a non-conventional oleaginous yeast that displays high lipid titers and yields; its production capacity holds significant promise for industrial biolipid applications. While its lipid metabolism has been widely studied, little is known about its transcriptional regulatory network. Deciphering the role of transcriptional regulators is crucial for understanding lipid accumulation, a complex phenomenon.To identify the transcription factors involved in lipid metabolism, we developed a systematic overexpression approach for 148 putative transcription factors...
March 29, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29607450/multiple-reaction-monitoring-targeted-lc-ms-analysis-of-potential-cell-death-marker-proteins-for-increased-bioprocess-control
#19
Simone Albrecht, Christian Kaisermayer, David Reinhart, Monica Ambrose, Renate Kunert, Anna Lindeberg, Jonathan Bones
The monitoring of protein biomarkers for the early prediction of cell stress and death is a valuable tool for process characterization and efficient biomanufacturing control. A representative set of six proteins, namely GPDH, PRDX1, LGALS1, CFL1, TAGLN2 and MDH, which were identified in a previous CHO-K1 cell death model using discovery LC-MSE was translated into a targeted liquid chromatography multiple reaction monitoring mass spectrometry (LC-MRM-MS) platform and verified. The universality of the markers was confirmed in a cell growth model for which three Chinese hamster ovary host cell lines (CHO-K1, CHO-S, CHO-DG44) were grown in batch culture in two different types of basal media...
April 2, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29605937/yield-enhancement-strategies-of-rare-pharmaceutical-metabolites-from-endophytes
#20
REVIEW
Fangxue Xu, Shiyuan Wang, Yujuan Li, Mengmeng Zheng, Xiaozhi Xi, Hui Cao, Xiaowei Cui, Hong Guo, Chunchao Han
Endophytes are barely untapped as vital sources in the medicine. They are microorganisms which mostly exist in plants. As they are exploited, it is accepted that endophytes can produce active metabolites that possess same function as their hosts such as taxol, podophyllotoxin, hypericin, and azadirachtin. These metabolites have been promising potential usefulness in safety and human health concerns. We are supposed to adopt measures to raise production for the low yield of metabolites. This paper summarizes the latest advances in various bioprocess optimization strategies...
March 31, 2018: Biotechnology Letters
keyword
keyword
13128
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"