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Bioprocessing

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https://www.readbyqxmd.com/read/28731612/mesoderm-lineage-3d-tissue-constructs-are-produced-at-large-scale-in-a-3d-stem-cell-bioprocess
#1
Jae Min Cha, Athanasios Mantalaris, Sunyoung Jung, Yurim Ji, Oh Young Bang, Hojae Bae
Various studies have presented different approaches to direct pluripotent stem cell differentiation such as applying defined sets of exogenous biochemical signals and genetic/epigenetic modifications. Although differentiation to target lineages was successfully regulated, such conventional methods are often complicated, laborious, and not cost-effective to be employed to the large-scale production of 3D stem cell-based tissue constructs. In this study, we developed a 3D-culture platform that could realize the large-scale production of mesoderm lineage tissue constructs from embryonic stem cells (ESCs)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731532/formate-and-nitrate-utilization-in-enterobacter-aerogenes-for-semi-anaerobic-production-of-isobutanol
#2
Hwi-Min Jung, Yong Hwan Kim, Min-Kyu Oh
Anaerobic bioprocessing is preferred because of its economic advantages. However, low productivity and decreased growth of the host strain have limited the use of the anaerobic process. Anaerobic respiration can be applied to anoxic processing using formate and nitrate metabolism to improve the productivity of value-added metabolites. We constructed isobutanol-producing strains using Enterobacter aerogenes as a host strain by metabolic engineering approaches. The byproduct pathway (ldhA, budA, and pflB) was knocked out, and heterologous keto-acid decarboxylase (kivD) and alcohol dehydrogenase (adhA) were expressed along with the L-valine synthesis pathway (ilvCD and budB)...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28726331/novel-fluid-shear-based-dissociation-device-for-improved-single-cell-dissociation-of-spheroids-and-cell-aggregates
#3
Ursula L Triantafillu, Jaron N Nix, Yonghyun Kim
Biological industries commonly rely on bioreactor systems for the large-scale production of cells. Cell aggregation, clumping, and spheroid morphology of certain suspension cells make their large-scale culture challenging. Growing stem cells as spheroids is indispensable to retain their stemness, but large spheroids (>500 µm diameter) suffer from poor oxygen and nutrient diffusion, ultimately resulting in premature cell death in the centers of the spheroids. Despite this, most large-scale bioprocesses do not have an efficient method for dissociating cells into single cells, but rely on costly enzymatic dissociation techniques...
July 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28725194/sophisticated-cloning-fermentation-and-purification-technologies-for-an-enhanced-therapeutic-protein-production-a-review
#4
REVIEW
Sanjeev K Gupta, Pratyoosh Shukla
The protein productions strategies are crucial towards the development of application based research and elucidating the novel purification strategies for industrial production. Currently, there are few innovative avenues are studies for cloning, upstream, and purification through efficient bioprocess development. Such strategies are beneficial for industries as well as proven to be vital for effectual therapeutic protein development. Though, these techniques are well documented, but, there is scope of addition to current knowledge with novel and new approaches and it will pave new avenues in production of recombinant microbial and non-microbial proteins including secondary metabolites...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28721445/a-two-step-bioprocessing-strategy-in-pentonic-acids-production-from-lignocellulosic-pre-hydrolysate
#5
Xin Zhou, Lu Huang, Yong Xu, Shiyuan Yu
Efficient utilization (over needless disposal) of biorefinery pre-hydrolysate is an economically relevant practice for improving biorefinery financial prospects. The liquid fraction obtained after acid hydrolysis pretreatment of lignocellulosic biomass, called pre-hydrolysate, are predominantly comprised of hemicellulosic carbohydrates. Using a two-step bioprocess, the hexoses were selectively fermented to ethanol by S. cerevisiae to clear the path for Gluconobacter oxydans transformation of pentoses to a high purity pentonic acids solution...
July 18, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28720107/reversible-bacterial-immobilization-based-on-the-salt-dependent-adhesion-of-the-bacterionanofiber-protein-ataa
#6
Shogo Yoshimoto, Yuki Ohara, Hajime Nakatani, Katsutoshi Hori
BACKGROUND: Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in the inner part of a gel, gel fragility, cell leakage from the support matrix, and adverse effects on cell viability and catalytic activity...
July 18, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28719144/maximizing-the-stability-of-metabolic-engineering-derived-whole-cell-biocatalysts
#7
REVIEW
Marvin Kadisch, Christian Willrodt, Michael Hillen, Bruno Bühler, Andreas Schmid
A systematic and powerful knowledge-based framework exists for improving the activity and stability of chemical catalysts and for empowering the commercialization of respective processes. In contrast, corresponding biotechnological processes are still scarce and characterized by case-by-case development strategies. A systematic understanding of parameters affecting biocatalyst efficiency, that is, biocatalyst activity and stability, is essential for a rational generation of improved biocatalysts. Today, systematic approaches only exist for increasing the activity of whole-cell biocatalysts...
July 18, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28716035/systematic-evaluation-of-characteristics-of-the-membrane-based-fed-batch-shake-flask
#8
P Philip, K Meier, D Kern, J Goldmanns, F Stockmeier, C Bähr, J Büchs
BACKGROUND: The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted with a central feed reservoir with an integrated rotating membrane tip for a controlled substrate release...
July 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28710856/quantitative-intracellular-flux-modeling-and-applications-in-biotherapeutic-development-and-production-using-cho-cell-cultures
#9
REVIEW
Zhuangrong Huang, Dong-Yup Lee, Seongkyu Yoon
Chinese hamster ovary (CHO) cells have been widely used for producing many recombinant therapeutic proteins. Constraint-based modeling, such as flux balance analysis (FBA) and metabolic flux analysis (MFA), has been developing rapidly for the quantification of intracellular metabolic flux distribution at a systematic level. Such methods would produce detailed maps of flows through metabolic networks, which contribute significantly to better understanding of metabolism in cells. Although these approaches have been extensively established in microbial systems, their application to mammalian cells is sparse...
July 15, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28710617/steroid-bioconversions
#10
Marina V Donova
Steroid modifications by selected wild-type and engineered strains of microorganisms became an effective tool for the production of high-valued steroidal drugs and their precursors for the pharmaceutical industry. Some microorganisms are effective at the performance of sterol side-chain degradation, oxyfunctionalization of steroid core, and redox reactions at different positions of the steroid molecule. A number of bioprocesses using steroid-transforming microbial strains are well established on an industrial level...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28700035/excavation-of-attractor-modules-for-nasopharyngeal-carcinoma-via-integrating-systemic-module-inference-with-attract-method
#11
T Jiang, C-Y Jiang, J-H Shu, Y-J Xu
The molecular mechanism of nasopharyngeal carcinoma (NPC) is poorly understood and effective therapeutic approaches are needed. This research aimed to excavate the attractor modules involved in the progression of NPC and provide further understanding of the underlying mechanism of NPC. Based on the gene expression data of NPC, two specific protein-protein interaction networks for NPC and control conditions were re-weighted using Pearson correlation coefficient. Then, a systematic tracking of candidate modules was conducted on the re-weighted networks via cliques algorithm, and a total of 19 and 38 modules were separately identified from NPC and control networks, respectively...
July 10, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28697935/continuous-countercurrent-tangential-chromatography-for-mixed-mode-post-capture-operations-in-monoclonal-antibody-purification
#12
Amit K Dutta, Dmitriy Fedorenko, Jasmine Tan, Joseph A Costanzo, David S Kahn, Andrew L Zydney, Oleg Shinkazh
Continuous Countercurrent Tangential Chromatography (CCTC) has been shown to demonstrate significant advantages over column chromatography including higher productivity, lower operational pressure, disposable flow path, and lower resin use. Previous applications of CCTC have been limited to initial capture of monoclonal antibodies (mAb) from clarified cell culture harvest. In this present article, a CCTC system was designed and tested for a post-capture antibody purification step. Mixed mode cation exchange-hydrophobic interaction chromatography resins with two different particle sizes were used to reduce host cell protein (HCP), leached protein A, DNA, and aggregates from a mAb stream after a protein A operation...
June 22, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28696059/metal-and-metalloid-biorecovery-using-fungi
#13
Xinjin Liang, Geoffrey Michael Gadd
Bioleaching is a proven bioprocess for metal recovery by solution from solid matrices, while a bioprecipitation or biomineralization approach is of potential for biorecovery from solution. Fungi can directly and indirectly mediate the formation of many kinds of minerals, including oxides, phosphates, carbonates and oxalates, as well as elemental forms of metals and metalloids such as Ag, Se and Te. Fungal capabilities may offer a potentially useful contribution to biotechnological and physico-chemical methods for metal recovery...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28690158/continuous-volatile-fatty-acid-production-from-lignocellulosic-biomass-by-a-novel-rumen-mimetic-bioprocess
#14
Hitosi Agematu, Takehiko Takahashi, Yoshio Hamano
Lignocellulosic biomass is an attractive source of biofuels and biochemicals, being abundant in various plant sources. However, processing this type of biomass requires hydrolysis of cellulose. The proposed rumen-mimetic bioprocess consists of dry-pulverization of lignocellulosic biomass and pH-controlled continuous cultivation of ruminal bacteria using ammonium as a nitrogen source. In this study, ruminal bacteria were continuously cultivated for over 60 days and used to digest microcrystalline cellulose, rice straw, and Japanese cedar to produce volatile fatty acids (VFAs)...
July 6, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28689014/poly-pathway-model-a-novel-approach-to-simulate-multiple-metabolic-states-by-reaction-network-based-model-application-to-amino-acid-depletion-in-cho-cell-culture
#15
Erika Hagrot, Hildur Æsa Oddsdóttir, Joan Gonzalez Hosta, Elling W Jacobsen, Véronique Chotteau
Mammalian cell lines are characterized by a complex and flexible metabolism. A single model that could describe the variations in metabolic behavior triggered by variations in the culture conditions would be a precious tool in bioprocess development. In this paper, we introduce an approach to generate a poly-pathway model and use it to simulate diverse metabolic states triggered in response to removal, reduction or doubling of amino acids in the culture medium of an antibody-producing CHO cell line. Macro-reactions were obtained from a metabolic network via elementary flux mode enumeration and the fluxes were modeled by kinetic equations with saturation and inhibition effects from external medium components...
July 5, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28687036/photosynthetic-poly-%C3%AE-hydroxybutyrate-accumulation-in-unicellular-cyanobacterium-synechocystis-sp-pcc-6714
#16
Donya Kamravamanesh, Stefan Pflügl, Winfried Nischkauer, Andreas Limbeck, Maximilian Lackner, Christoph Herwig
Poly-β-hydroxybutyrate (PHB) production from CO2 has the potential to reduce the production cost of this biodegradable polyesters, and also to make the material more sustainable compared to utilization of sugar feedstocks. In this study the unicellular cyanobacterium, Synechocystis sp. PCC 6714 has been identified as an unexplored potential organism for production of PHB. Synechocystis sp. PCC 6714 was studied under various cultivation conditions and nutritional limitations. Combined effects of nitrogen and phosphorus deficiency led to highest PHB accumulation under photoautotrophic conditions...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28685239/a-biodesign-approach-to-obtain-high-yields-of-biosimilars-by-anti-apoptotic-cell-engineering-a-case-study-to-increase-the-production-yield-of-anti-tnf-alpha-producing-recombinant-cho-cells
#17
Sultan Gulce Iz, Muge Anil Inevi, Pelin Saglam Metiner, Duygu Ayyildiz Tamis, Nazli Kisbet
Recent developments in medical biotechnology have facilitated to enhance the production of monoclonal antibodies (mAbs) and recombinant proteins in mammalian cells. Human mAbs for clinical applications have focused on three areas, particularly cancer, immunological disorders, and infectious diseases. Tumor necrosis factor alpha (TNF-α), which has both proinflammatory and immunoregulatory functions, is an important target in biopharmaceutical industry. In this study, a humanized anti-TNF-α mAb producing stable CHO cell line which produces a biosimilar of Humira (adalimumab) was used...
July 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28678828/a-technique-for-lyopreservation-of-clostridium-ljungdahlii-in-a-biocomposite-matrix-for-co-absorption
#18
Mark J Schulte, Jason Solocinski, Mian Wang, Michelle Kovacs, Ryan Kilgore, Quinn Osgood, Lukas Underwood, Michael C Flickinger, Nilay Chakraborty
A system capable of biocatalytic conversion of distributed sources of single carbon gases such as carbon monoxide into hydrocarbons can be highly beneficial for developing commercially viable biotechnology applications in alternative energy. Several anaerobic bacterial strains can be used for such conversion. The anaerobic carbon monoxide-fixing bacteria Clostridium ljungdahlii OTA1 is a model CO assimilating microorganism that currently requires cryogenic temperature for storage of the viable strains. If these organisms can be stabilized and concentrated in thin films in advanced porous materials, it will enable development of high gas fraction, biocomposite absorbers with elevated carbon monoxide (CO) mass transfer rate, that require minimal power input and liquid, and demonstrate elevated substrate consumption rate compared to conventional suspended cell bioreactors...
2017: PloS One
https://www.readbyqxmd.com/read/28674730/cauliflower-waste-utilization-for-sustainable-biobutanol-production-revelation-of-drying-kinetics-and-bioprocess-development
#19
Manisha A Khedkar, Pranhita R Nimbalkar, Prakash V Chavan, Yogesh J Chendake, Sandip B Bankar
Efficient yet economic production of biofuel(s) using varied second-generation feedstock needs to be explored in the current scenario to cope up with global fuel demand. Hence, the present study was performed to reveal the use of cauliflower waste for acetone-butanol-ethanol (ABE) production using Clostridium acetobutylicum NRRL B 527. The proximate analysis of cauliflower waste demonstrated to comprise 17.32% cellulose, 9.12% hemicellulose, and 5.94% lignin. Drying of cauliflower waste was carried out in the temperature range of 60-120 °C to investigate its effect on ABE production...
July 3, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28671262/an-efficient-model-construction-strategy-to-simulate-microalgal-lutein-photo-production-dynamic-process
#20
Ehecatl Antonio Del Rio-Chanona, Fabio Fiorelli, Dongda Zhang, Nur Rashid Ahmed, Keju Jing, Nilay Shah
Lutein is a high-value bioproduct synthesised by microalga Desmodesmus sp.. It has great potential for the food, cosmetics, and pharmaceutical industries. However, in order to enhance its productivity and to fulfil its ever-increasing global market demand, it is vital to construct accurate models capable of simulating the entire behaviour of the complicated dynamics of the underlying biosystem. To this aim, in this study two highly robust artificial neural networks are designed for the first time. Contrary to conventional artificial neural networks, these networks model the rate of change of the dynamic system, which makes them highly relevant in practice...
July 3, 2017: Biotechnology and Bioengineering
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