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Bioprocessing

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https://www.readbyqxmd.com/read/29055113/accelerating-patient-access-to-novel-biologics-using-stable-pool-derived-product-for-non-clinical-studies-and-single-clone-derived-product-for-clinical-studies
#1
Trent P Munro, Kim Le, Huong Le, Li Zhang, Jennitte Stevens, Neil Soice, Sabrina A Benchaar, Robert W Hong, Chetan T Goudar
Cell cloning and subsequent process development activities are on the critical path directly impacting the timeline for advancement of next generation therapies to patients with unmet medical needs. The use of stable cell pools for early stage material generation and process development activities is an enabling technology to reduce timelines. To successfully use stable pools during development, it is important that bioprocess performance and requisite product quality attributes be comparable to those observed from clonally derived cell lines...
October 21, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29042054/preface-special-issue-on-advances-in-industrial-bioprocesses-and-products-genetic-and-metabolic-engineering-interventions
#2
EDITORIAL
Michael Sauer, Akihiko Kondo, Guocheng Du, Madhavan Nampoothiri
No abstract text is available yet for this article.
December 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29040886/role-of-n-6-methyladenosine-modification-in-cancer
#3
REVIEW
Xiaolan Deng, Rui Su, Xuesong Feng, Minjie Wei, Jianjun Chen
As the most abundant internal modification in eukaryotic messenger RNAs identified, N(6)-methyladenosine (m(6)A) has been shown recently to play essential roles in various normal bioprocesses. Evidence is emerging that m(6)A modification and its regulatory proteins also play critical roles in various cancers including leukemia, brain tumor, breast cancer and lung cancer, etc. For instance, FTO, the first m(6)A demethylase identified, has been reported recently to play an oncogenic role in leukemia and glioblastoma...
October 14, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/29040308/thermostable-proteins-bioprocesses-the-activity-of-restriction-endonuclease-methyltransferase-from-thermus-thermophilus-rm-tthhb27i-cloned-in-escherichia-coli-is-critically-affected-by-the-codon-composition-of-the-synthetic-gene
#4
Daria Krefft, Aliaksei Papkov, Agnieszka Zylicz-Stachula, Piotr M Skowron
Obtaining thermostable enzymes (thermozymes) is an important aspect of biotechnology. As thermophiles have adapted their genomes to high temperatures, their cloned genes' expression in mesophiles is problematic. This is mainly due to their high GC content, which leads to the formation of unfavorable secondary mRNA structures and codon usage in Escherichia coli (E. coli). RM.TthHB27I is a member of a family of bifunctional thermozymes, containing a restriction endonuclease (REase) and a methyltransferase (MTase) in a single polypeptide...
2017: PloS One
https://www.readbyqxmd.com/read/29037147/de-novo-assembly-of-genomes-from-long-sequence-reads-reveals-uncharted-territories-of-propionibacterium-freudenreichii
#5
Paulina Deptula, Pia K Laine, Richard J Roberts, Olli-Pekka Smolander, Helena Vihinen, Vieno Piironen, Lars Paulin, Eija Jokitalo, Kirsi Savijoki, Petri Auvinen, Pekka Varmanen
BACKGROUND: Propionibacterium freudenreichii is an industrially important bacterium granted the Generally Recognized as Safe (the GRAS) status, due to its long safe use in food bioprocesses. Despite the recognized role in the food industry and in the production of vitamin B12, as well as its documented health-promoting potential, P. freudenreichii remained poorly characterised at the genomic level. At present, only three complete genome sequences are available for the species. RESULTS: We used the PacBio RS II sequencing platform to generate complete genomes of 20 P...
October 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29034620/-progress-in-chemicals-production-by-microbial-consortia
#6
Lili Jiang, Jinjie Zhou, Xudong Wang, Yaqin Sun, Zhilong Xiu
Using cheap biomass resources is a hotspot of research on industrial biotechnology. It is difficult for traditional fermentations with single strain to treat so complex components and more impurities, which becomes the key problem in industrialization. In this review, some existing industrial bioprocesses involving microbial consortia were described. Comparison of 1,3-propanediol production by microbial consortia and pure cultures were then introduced and the relationship between cells in microbial consortia were summarized...
November 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29033837/dioscin-inhibits-hsc-t6-cell-migration-via-adjusting-sdc-4-expression-insights-from-itraq-based-quantitative-proteomics
#7
Lianhong Yin, Yan Qi, Youwei Xu, Lina Xu, Xu Han, Xufeng Tao, Shasha Song, Jinyong Peng
Hepatic stellate cells (HSCs) migration, an important bioprocess, contributes to the development of liver fibrosis. Our previous studies have found the potent activity of dioscin against liver fibrosis by inhibiting HSCs proliferation, triggering the senescence and inducing apoptosis of activated HSCs, but the molecular mechanisms associated with cell migration were not clarified. In this work, iTRAQ (isobaric tags for relative and absolution quantitation)-based quantitative proteomics study was carried out, and a total of 1566 differentially expressed proteins with fold change ≥2...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29030621/flow-cytometry-as-a-rapid-analytical-tool-to-determine-physiological-responses-to-changing-o2-and-iron-concentration-by-magnetospirillum-gryphiswaldense-strain-msr-1
#8
Alfred Fernández-Castané, Hong Li, Owen R T Thomas, Tim W Overton
Magnetotactic bacteria (MTB) are a diverse group of bacteria that synthesise magnetosomes, magnetic membrane-bound nanoparticles that have a variety of diagnostic, clinical and biotechnological applications. We present the development of rapid methods using flow cytometry to characterize several aspects of the physiology of the commonly-used MTB Magnetospirillum gryphiswaldense MSR-1. Flow cytometry is an optical technique that rapidly measures characteristics of individual bacteria within a culture, thereby allowing determination of population heterogeneity and also permitting direct analysis of bacteria...
October 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29027766/kinetic-modeling-of-mammalian-cell-culture-bioprocessing-the-quest-to-advance-biomanufacturing
#9
REVIEW
Sarantos Kyriakopoulos, Kok Siong Ang, Meiyappan Lakshmanan, Zhuangrong Huang, Seongkyu Yoon, Rudiyanto Gunawan, Dong-Yup Lee
Kinetic modeling is the most suitable framework to describe the dynamic behavior of mammalian cell culture although its industrial application is still in its infancy. Herein, we review mammalian bioprocess relevant kinetic models and found that the simple unstructured-unsegregated approach utilizing empirical Monod type kinetics based on limiting substrates and inhibitory metabolites is commonly used due to the traceability and simple formalism. Notably, the available kinetic models are typically small to moderate in size and the development of large-scale models is severely hampered by the scarcity of kinetic data and limitations in current parameter estimation methods...
October 13, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29024819/a-modular-metabolic-engineering-approach-for-the-production-of-1-2-propanediol-from-glycerol-by-saccharomyces-cerevisiae
#10
Zia-Ul Islam, Mathias Klein, Maximilian R Aßkamp, Anders S R Ødum, Elke Nevoigt
Compared to sugars, a major advantage of using glycerol as a feedstock for industrial bioprocesses is the fact that this molecule is more reduced than sugars. A compound whose biotechnological production might greatly profit from the substrate's higher reducing power is 1,2-propanediol (1,2-PDO). Here we present a novel metabolic engineering approach to produce 1,2-PDO from glycerol in S. cerevisiae. Apart from implementing the heterologous methylglyoxal (MG) pathway for 1,2-PDO formation from dihydroxyacetone phosphate (DHAP) and expressing a heterologous glycerol facilitator, the employed genetic modifications included the replacement of the native FAD-dependent glycerol catabolic pathway by the `DHA pathway´ for delivery of cytosolic NADH and the reduction of triosephosphate isomerase (TPI) activity for increased precursor (DHAP) supply...
October 9, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/29020992/facile-aerobic-construction-of-iron-based-ferromagnetic-nanostructures-by-a-novel-microbial-nanofactory-isolated-from-tropical-freshwater-wetlands
#11
Patricia Jayshree Jacob, Mas Jaffri Masarudin, Mohd Zobir Hussein, Raha Abdul Rahim
BACKGROUND: Iron based ferromagnetic nanoparticles (IONP) have found a wide range of application in microelectronics, chemotherapeutic cell targeting, and as contrast enhancers in MRI. As such, the design of well-defined monodisperse IONPs is crucial to ensure effectiveness in these applications. Although these nanostructures are currently manufactured using chemical and physical processes, these methods are not environmentally conducive and weigh heavily on energy and outlays. Certain microorganisms have the innate ability to reduce metallic ions in aqueous solution and generate nano-sized IONP's with narrow size distribution...
October 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29019120/two-dimensional-gel-electrophoresis-and-2d-dige
#12
Paula Meleady
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to be one of the most versatile and widely used techniques to study the proteome of a biological system. In particular, a modified version of 2D-PAGE, two-dimensional difference gel electrophoresis (2D-DIGE), which uses differential labeling of protein samples with up to three fluorescent tags, offers greater sensitivity and reproducibility over conventional 2D-PAGE gels for differential quantitative analysis of protein expression between experimental groups...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29017530/customized-microscale-approach-for-optimizing-two-phase-bio-oxidations-of-alkanes-with-high-reproducibility
#13
Johannes F Kolmar, Oliver Thum, Frank Baganz
BACKGROUND: Numerous challenges remain to achieve industrially competitive space-time yields for bio-oxidations. The ability to rapidly screen bioconversion reactions for characterization and optimization is of major importance in bioprocess development and biocatalyst selection; studies at conventional lab scale are time consuming and labor intensive with low experimental throughput. The direct ω-oxyfunctionalization of aliphatic alkanes in a regio- and chemoselective manner is efficiently catalyzed by monooxygenases such as the AlkBGT enzyme complex from Pseudomonas putida under mild conditions...
October 10, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28988049/development-of-a-thermo-stable-and-recyclable-magnetic-nanobiocatalyst-for-bioprocessing-of-fruit-processing-residues-and-d-allulose-synthesis
#14
Satya Narayan Patel, Vishal Singh, Manisha Sharma, Rajender S Sangwan, Nitin K Singhal, Sudhir P Singh
The aim of the study was to covalently immobilize Smt3-D-psicose 3-epimerase onto functionalized iron oxide magnetic nanoparticles. After immobilization, Km of the immobilized enzyme increased, however, Vmax was nearly the same as that of its free form, indicating that immobilization has no detrimental effects on its catalytic output. The covalent immobilization caused a reduction in the deactivation rate constant (kd) values leading to 4-5 fold enhancement in its half-life at 50-65°C, indicating significant thermal stability of the iron-enzyme nanobioconjugate...
September 20, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28987922/the-impact-of-systems-biology-on-bioprocessing
#15
REVIEW
Kate Campbell, Jianye Xia, Jens Nielsen
Bioprocessing offers a sustainable and green approach to the production of chemicals. However, a bottleneck in introducing bioprocesses is cell factory development, which is costly and time-consuming. A systems biology approach can expedite cell factory design by using genome-wide analyses alongside mathematical modeling to characterize and predict cellular physiology. This approach can drive cycles of design, build, test, and learn implemented by metabolic engineers to optimize the cell factory performance...
October 4, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28987025/an-integrated-bioprocess-for-the-expansion-and-chondrogenic-priming-of-human-periosteum-derived-progenitor-cells-in-suspension-bioreactors
#16
Priyanka Gupta, Liesbet Geris, Frank P Luyten, Ioannis Papantoniou
The increasing use of microcarrier based suspension bioreactors for scalable expansion of adult progenitor cells in recent years reveals the necessity of such approaches to address bio manufacturing challenges of advanced therapeutic medicinal products. However, the differentiation of progenitor cells within suspension bioreactors for the production of tissue modules is of equal importance but not well investigated. This study reports on the development of a bioreactor based integrated process for expansion and chondrogenic priming of human periosteum derived stem cells (hPDCs) using Cultispher S microcarriers...
October 7, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28983758/biotechnological-production-of-bio-based-long-chain-dicarboxylic-acids-with-oleogenious-yeasts
#17
REVIEW
Nicole Werner, Susanne Zibek
Long-chain α,ω-dicarboxylic acids (DCAs) are versatile chemical intermediates of industrial importance used as building blocks for the production of polymers, lubricants, or adhesives. The majority of industrial long-chain DCAs is produced from petro-chemical resources. An alternative is their biotechnological production from renewable materials like plant oil fatty acids by microbial fermentation using oleogenious yeasts. Oleogenious yeasts are natural long-chain DCA producers, which have to be genetically engineered for high-yield DCA production...
October 5, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28982254/biotransformation-of-cubebin-by-aspergillus-spp-into-hinokinin-and-parabenzlactone-and-their-evaluation-against-oral-pathogenic-bacteria
#18
Caroline Arruda, Daniele de Souza Eugênio, Monique Rodrigues Moreira, Guilherme Venâncio Símaro, Jairo Kenupp Bastos, Carlos Henrique Gomes Martins, Márcio Luís Andrade Silva, Rodrigo Cássio Sola Veneziani, Paula Bruzadelle Vieira, Sérgio R Ambrósio
The biotransformation of the lignan (-)-cubebin by filamentous fungi Aspergillus terreus and Aspergillus niger is an efficient bioprocess for obtaining (-)-hinokinin and (-)-parabenzlactone. The relevance of getting (-)-hinokinin is due to its promising effect against oral pathogens, especially S. sanguinis (both MIC and MBC 12.5 μg/mL), and other previous reported effects against Chagas disease and as anti-inflammatory. The advantage of using fungal transformation is the use of non-toxic and/or non-pollutant reagents and/or solvents in comparison with semi-synthesis...
October 6, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28981255/on-line-ion-exchange-liquid-chromatography-as-a-process-analytical-technology-for-monoclonal-antibody-characterization-in-continuous-bioprocessing
#19
Bhumit A Patel, Nuno D S Pinto, Adrian Gospodarek, Bruce Kilgore, Kudrat Goswami, William N Napoli, Jayesh Desai, Jun H Heo, Dominick Panzera, David Pollard, Daisy Richardson, Mark Brower, Douglas D Richardson
Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes. This work demonstrates on-line liquid chromatography (on-line LC) as a PAT tool for monitoring a continuous biologics process and forced degradation studies. Specifically, this work focused on ion exchange chromatography (IEX), which is a critical separation technique to detect charge variants. Product-related impurities, including charge variants, that impact function are classified as critical quality attributes (CQAs)...
October 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28973873/engineering-of-tomato-for-the-sustainable-production-of-ketocarotenoids-and-its-evaluation-in-aquaculture-feed
#20
Marilise Nogueira, Eugenia M A Enfissi, Maria E Martínez Valenzuela, Guillaume N Menard, Richard L Driller, Peter J Eastmond, Wolfgang Schuch, Gerhard Sandmann, Paul D Fraser
Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3...
September 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
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