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https://www.readbyqxmd.com/read/28636078/microbial-lipopeptide-production-and-purification-bioprocesses-current-progress-and-future-challenges
#1
REVIEW
François Coutte, Didier Lecouturier, Krasimir Dimitrov, Jean-Sébastien Guez, Frank Delvigne, Pascal Dhulster, Philippe Jacques
Lipopeoptides are amphiphilic compounds combining interesting physicochemical properties and biological activities. Due to their high foaming capacity in aerated bioreactor, the development of scalable bioprocesses for their production is a major bottleneck. In addition, the genes involved in the biosynthesis of these lipopeptides are mainly regulated by the quorum sensing, a global regulatory mechanism depending on cell density and known to be activated in biofilms. Several approaches have thus been considered in literature taking into account two criteria, on one side, to favor, control or avoid foam formation and on the other side, to use planktonic or immobilized (biofilm) cells...
June 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28634129/state-of-the-art-review-of-biofuels-production-from-lignocellulose-by-thermophilic-bacteria
#2
REVIEW
Yujia Jiang, Fengxue Xin, Jiasheng Lu, Weiliang Dong, Wenming Zhang, Min Zhang, Hao Wu, Jiangfeng Ma, Min Jiang
Biofuels, including ethanol and butanol, are mainly produced by mesophilic solventogenic yeasts and Clostridium species. However, these microorganisms cannot directly utilize lignocellulosic materials, which are abundant, renewable and non-compete with human demand. More recently, thermophilic bacteria show great potential for biofuels production, which could efficiently degrade lignocellulose through the cost effective consolidated bioprocessing. Especially, it could avoid contamination in the whole process owing to its relatively high fermentation temperature...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28630295/application-of-metabolic-controls-for-the-maximization-of-lipid-production-in-semicontinuous-fermentation
#3
Jingyang Xu, Nian Liu, Kangjian Qiao, Sebastian Vogg, Gregory Stephanopoulos
Acetic acid can be generated through syngas fermentation, lignocellulosic biomass degradation, and organic waste anaerobic digestion. Microbial conversion of acetate into triacylglycerols for biofuel production has many advantages, including low-cost or even negative-cost feedstock and environmental benefits. The main issue stems from the dilute nature of acetate produced in such systems, which is costly to be processed on an industrial scale. To tackle this problem, we established an efficient bioprocess for converting dilute acetate into lipids, using the oleaginous yeast Yarrowia lipolytica in a semicontinuous system...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28627713/process-development-of-human-multipotent-stromal-cell-microcarrier-culture-using-an-automated-high-throughput-microbioreactor
#4
Qasim A Rafiq, Mariana P Hanga, Thomas R J Heathman, Karen Coopman, Alvin W Nienow, David J Williams, Christopher J Hewitt
Microbioreactors play a critical role in process development as they reduce reagent requirements and can facilitate high-throughput screening of process parameters and culture conditions. Here we have demonstrated and explained in detail, for the first time, the amenability of the automated ambr15 cell culture microbioreactor system for the development of scalable adherent human mesenchymal multipotent stromal/stem cell (hMSC) microcarrier culture processes. This was achieved by first improving suspension and mixing of the microcarriers and then improving cell attachment thereby reducing the initial growth lag phase...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28625827/cells-as-advanced-therapeutics-state-of-the-art-challenges-and-opportunities-in-large-scale-biomanufacturing-of-high-quality-cells-for-adoptive-immunotherapies
#5
Nate J Dwarshuis, Kirsten Parratt, Adriana Santiago-Miranda, Krishnendu Roy
Therapeutic cells hold tremendous promise in treating currently incurable, chronic diseases since they perform multiple, integrated, complex functions in vivo compared to traditional small-molecule drugs or biologics. However, they also pose significant challenges as therapeutic products because (a) their complex mechanisms of actions are difficult to understand and (b) low-cost bioprocesses for large-scale, reproducible manufacturing of cells have yet to be developed. Immunotherapies using T cells and dendritic cells (DCs) have already shown great promise in treating several types of cancers, and human mesenchymal stromal cells (hMSCs) are now extensively being evaluated in clinical trials as immune-modulatory cells...
June 15, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28621179/an-integrated-experimental-and-economic-evaluation-of-cell-therapy-affinity-purification-technologies
#6
Benjamin D Weil, Michael J Jenkins, Siddique Uddin, Daniel G Bracewell, Donald Wellings, Suzanne S Farid, Farlan Veraitch
AIM: To present an integrated techno-economic analysis assessing the feasibility of affinity purification technologies using the manufacture of induced pluripotent stem cell-derived progenitor photoreceptors for retinal dystrophies as a case study. MATERIALS & METHODS: Sort purity, progenitor yield and viable cell recovery were investigated for three cell sorting techniques: fluorescent-activated cell sorting (FACS); magnetic-activated cell sorting (MACS); and a novel technology SpheriTech beads...
June 16, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28620039/csbb-synthetic-biology-research-at-newcastle-university
#7
EDITORIAL
Angel Goñi-Moreno, Anil Wipat, Natalio Krasnogor
The Centre for Synthetic Biology and the Bioeconomy (CSBB) brings together a far-reaching multidisciplinary community across all Newcastle University's faculties - Medical Sciences, Science, Agriculture and Engineering, and Humanities, Arts and Social Sciences. The CSBB focuses on many different areas of Synthetic Biology, including bioprocessing, computational design and in vivo computation, as well as improving understanding of basic molecular machinery. Such breadth is supported by major national and international research funding, a range of industrial partners in the North East of England and beyond, as well as a large number of doctoral and post-doctoral researchers...
June 15, 2017: Biochemical Society Transactions
https://www.readbyqxmd.com/read/28616074/thermodynamics-and-economic-feasibility-of-acetone-production-from-syngas-using-the-thermophilic-production-host-moorella-thermoacetica
#8
Stephanie Redl, Sumesh Sukumara, Tom Ploeger, Liang Wu, Torbjørn Ølshøj Jensen, Alex Toftgaard Nielsen, Henk Noorman
BACKGROUND: Syngas fermentation is a promising option for the production of biocommodities due to its abundance and compatibility with anaerobic fermentation. Using thermophilic production strains in a syngas fermentation process allows recovery of products with low boiling point from the off-gas via condensation. RESULTS: In this study we analyzed the production of acetone from syngas with the hypothetical production host derived from Moorella thermoacetica in a bubble column reactor at 60 °C with respect to thermodynamic and economic feasibility...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616072/the-advanced-strategy-for-enhancing-biobutanol-production-and-high-efficient-product-recovery-with-reduced-wastewater-generation
#9
Chuang Xue, Xiaotong Zhang, Jufang Wang, Min Xiao, Lijie Chen, Fengwu Bai
BACKGROUND: Butanol as an important chemical and potential fuel could be produced via ABE fermentation from lignocellulosic biomass. The use of food-related feedstocks such as maize and sugar cane may not be a sustainable solution to world's energy needs. Recently, Jerusalem artichoke tubers containing inulin have been used as feedstock for butanol production, but this bioprocess is not commercially feasible due to the great value of inulin as functional food. Till now, there is a gap on the utilization of Jerusalem artichoke stalk (JAS) as feedstock for microbial butanol production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28616071/combined-de-novo-and-ex-novo-lipid-fermentation-in-a-mix-medium-of-corncob-acid-hydrolysate-and-soybean-oil-by-trichosporon-dermatis
#10
Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Gao-Xiang Qi, Lian Xiong, Xiao-Qing Lin, Can Wang, Hai-Long Li, Xin-De Chen
BACKGROUND: Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and hydrophobic substrates can be utilized by oleaginous microorganisms with two different bio-pathways ("de novo" lipid fermentation and "ex novo" lipid fermentation). To date, most of the research on lipid fermentation has focused mainly on only one fermentation pathway and little work was carried out on both "de novo" and "ex novo" lipid fermentation simultaneously; thus, the advantages of both lipid fermentation cannot be fulfilled comprehensively...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28610972/development-and-evaluation-of-consolidated-bioprocessing-yeast-for-ethanol-production-from-ionic-liquid-pretreated-bagasse
#11
Jerome Amoah, Naoya Ishizue, Miki Ishizaki, Misa Yasuda, Kenji Takahashi, Kazuaki Ninomiya, Ryosuke Yamada, Akihiko Kondo, Chiaki Ogino
This work aimed to study the use of consolidated bioprocess (CBP) yeast expressing five cellulase genes (BGL, XYNII, EGII, CBHI and CBHII) for ethanol production from ionic liquid-pretreated bagasse and Laubholz unbleached Kraft pulp (LUKP). A proposed screening method shows that the optimal cellulase ratio varies for each biomass substrate, and thus it is essential to breed CBP yeast having optimal cellulase-displaying ratio for the target biomass. CBP yeast specialized towards bagasse produced 0.93g/l ethanol whiles that for LUKP produced 0...
May 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28609710/sense-and-sensitivity-in-bioprocessing-detecting-cellular-metabolites-with-biosensors
#12
REVIEW
Linda Dekker, Karen M Polizzi
Biosensors use biological elements to detect or quantify an analyte of interest. In bioprocessing, biosensors are employed to monitor key metabolites. There are two main types: fully biological systems or biological recognition coupled with physical/chemical detection. New developments in chemical biosensors include multiplexed detection using microfluidics. Synthetic biology can be used to engineer new biological biosensors with improved characteristics. Although there have been few biosensors developed for bioprocessing thus far, emerging trends can be applied in the future...
June 10, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28605325/turmeric-bioprocessed-with-mycelia-from-the-shiitake-culinary-medicinal-mushroom-lentinus-edodes-agaricomycetes-protects-mice-against-salmonellosis
#13
Sung Phil Kim, Sang Jong Lee, Seok Hyun Nam, Mendel Friedman
This study investigated the suppressive mechanisms of an extract from bioprocessed Lentinus edodes mycelial liquid culture supplemented with turmeric (bioprocessed Curcuma longa extract [BPCLE]) against murine salmonellosis. The BPLCE extract from the bioprocessed mycelia of the Salmonella Typhimurium into murine RAW 264.7 macrophage cells, elimination of intracellular bacteria, and elevation of inducible nitric oxide synthase expression. Dietary administration of BPCLE activated leukocytes from the mice infected with Salmonella through the intraperitoneal route...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/28594350/micro-electromechanical-affinity-sensor-for-the-monitoring-of-glucose-in-bioprocess-media
#14
Lorenz Theuer, Micha Lehmann, Stefan Junne, Peter Neubauer, Mario Birkholz
An affinity-viscometry-based micro-sensor probe for continuous glucose monitoring was investigated with respect to its suitability for bioprocesses. The sensor operates with glucose and dextran competing as binding partner for concanavalin A, while the viscosity of the assay scales with glucose concentration. Changes in viscosity are determined with a micro-electromechanical system (MEMS) in the measurement cavity of the sensor probe. The study aimed to elucidate the interactions between the assay and a typical phosphate buffered bacterial cultivation medium...
June 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28593603/optimization-of-inexpensive-agricultural-by-products-as-raw-materials-for-bacitracin-production-in-bacillus-licheniformis-dw2
#15
Qin Wang, Hui Zheng, Xia Wan, Hongfeng Huang, Junhui Li, Christopher T Nomura, Changjun Wang, Shouwen Chen
Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12...
June 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28585362/tailor-made-pat-platform-for-safe-syngas-fermentations-in-batch-fed-batch-and-chemostat-mode-with-rhodospirillum-rubrum
#16
Stephanie Karmann, Stéphanie Follonier, Daniel Egger, Dirk Hebel, Sven Panke, Manfred Zinn
Recently, syngas has gained significant interest as renewable and sustainable feedstock, in particular for the biotechnological production of poly([R]-3-hydroxybutyrate) (PHB). PHB is a biodegradable, biocompatible polyester produced by some bacteria growing on the principal component of syngas, CO. However, working with syngas is challenging because of the CO toxicity and the explosion danger of H2 , another main component of syngas. In addition, the bioprocess control needs specific monitoring tools and analytical methods that differ from standard fermentations...
June 6, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28583405/pre-aeration-of-food-waste-to-augment-acidogenic-process-at-higher-organic-load-valorizing-biohydrogen-volatile-fatty-acids-and-biohythane
#17
Omprakash Sarkar, S Venkata Mohan
Application of pre-aeration (AS) to waste prior to feeding was evaluated on acidogenic process in a semi-pilot scale biosystem for the production of biobased products (biohydrogen, volatile fatty acids (VFA) and biohythane) from food waste. Oxygen assisted in pre-hydrolysis of waste along with the suppression of methanogenic activity resulting in enhanced acidogenic product formation. AS operation resulted in 97% improvement in hydrogen conversion efficiency (HCE) and 10% more VFA production than the control...
May 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28578062/biotransformed-citrus-extract-as-a-source-of-anti-inflammatory-polyphenols-effects-in-macrophages-and-adipocytes
#18
Vânia Mayumi Nakajima, Tais Moala, Cintia Rabelo E Paiva Caria, Carolina Soares Moura, Jaime Amaya-Farfan, Alessandra Gambero, Gabriela Alves Macedo, Juliana Alves Macedo
Chronic non-communicable diseases such as obesity are preceded by increased macrophage infiltration in adipose tissue and greater secretion of pro-inflammatory cytokines. We evaluated the anti-inflammatory potential of Biotransformed extract, and two control extracts: In Natura and Autoclaved. The assays were performed using a cellular model with RAW264.7, 3T3-L1 cells, and RAW264.7 and 3T3-L1 co-culture. The innovation of the study was the use of Biotransformed extract, a unique phenolic extract of a bioprocessed citrus residue...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28571557/recent-advances-of-individual-bodipy-and-bodipy-based-functional-materials-in-medical-diagnostics-and-treatment
#19
Yuriy S Marfin, Alexey V Solomonov, Alexander S Timin, Evgeniy V Rumyantsev
The group of fluorophores on boron dipyrrin platform (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, also known as BODIPY) has attracted much attention in the field of molecular sensorics, including sensing of biomolecules and bioprocesses. Structural diversity of existing BODIPY with ample opportunities of directed modification of compounds makes this class of fluorophores attractive for medical and biological purposes. The recent progress in the design and functionalization of BODIPY allows to use them for modification of drug micro- and nanocarriers in order to improve their therapeutic effect in cancer treatment...
May 31, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28567672/bioprocess-engineering-to-produce-9-nonanoyloxy-nonanoic-acid-by-a-recombinant-corynebacterium-glutamicum-based-biocatalyst
#20
Hyeonsoo Kim, Soohyun Park, Sukhyeong Cho, Jeongmo Yang, Kijun Jeong, Jinbyung Park, Jinwon Lee
Here, Corynebacterium glutamicum ATCC13032 expressing Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. Diverse parameters including cultivation and reaction temperatures, type of detergent, and pH were found to improve biotransformation efficiency. The optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant C. glutamicum was 15 °C, but the reaction temperature was optimal at 30 °C...
June 1, 2017: Journal of Industrial Microbiology & Biotechnology
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