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https://www.readbyqxmd.com/read/27913897/rainbow-trout-oncorhynchus-mykiss-pro-oxidant-and-genotoxic-responses-following-acute-and-chronic-exposure-to-the-antibiotic-oxytetracycline
#1
Sara Rodrigues, Sara C Antunes, Alberto T Correia, Bruno Nunes
Oxytetracycline (OTC), an antibacterial agent, is extensively used in aquaculture practices all over the world, but also in human and veterinary medicines. Because of its intensive use, low rates of absorption by treated animals, inadequate disposal, and low efficiency of removal in wastewater treatment plants, the potential harmful effects on aquatic organisms are of great concern. This work aimed to assess the effects of this antibiotic in rainbow trout, following both acute and chronic exposures. Catalase (CAT), total glutathione peroxidase (GPx), glutathione reductase (GRed) activities and lipid peroxidation (TBARS levels) were quantified as oxidative stress biomarkers, in gills and liver...
December 2, 2016: Ecotoxicology
https://www.readbyqxmd.com/read/27912934/effect-of-sequential-treatment-with-tcm-syndrome-differentiation-on-acute-exacerbation-of-chronic-obstructive-pulmonary-disease-and-aecopd-risk-window
#2
Li Jiansheng, Wang Haifeng, Li Suyun, Zhang Hailong, Yu Xueqing, Zhang Xiaoyun, Li Fengsen, Zhou Xianmei, Sun Zikai, Mao Yimin, Ma Lijun, Zhang Yijie, Zhang Guojun, Tang Bingxiang
OBJECTIVE: To evaluate the efficacy and safety of the comprehensive interventions based on three Traditional Chinese medicine (TCM) patterns therapy in acute exacerbations of chronic obstructive pulmonary disease (AECOPD) and AECOPD risk window. METHODS: A prospective, multi-center, single-blinded, double-dummy and randomized controlled clinical trial is being conducted to test the therapeutic effects of a sequential two stage treatment. A total of 364 patients were enrolled into this study with 182 in each treatment group (TCM and conventional)...
December 2016: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/27907059/quantitative-and-selective-analysis-of-feline-growth-related-proteins-using-parallel-reaction-monitoring-high-resolution-mass-spectrometry
#3
Mårten Sundberg, Emma M Strage, Jonas Bergquist, Bodil S Holst, Margareta Ramström
Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM) methods...
2016: PloS One
https://www.readbyqxmd.com/read/27905920/synergistic-effects-and-related-bioactive-mechanism-of-potentilla-fruticosa-l-leaves-combined-with-ginkgo-biloba-extracts-studied-with-microbial-test-system-mts
#4
Ze-Hua Liu, Dong-Mei Wang, Su-Fang Fan, Deng-Wu Li, Zi-Wen Luo
BACKGROUND: Potentilla fruticosa, also called "Jinlaomei" and "Gesanghua", is widely used as folk herbs in traditional Tibetan medicine in China to treat inflammations, wounds, certain forms of cancer, diarrhoea, diabetes and other ailments. Previous research found P. fruticosa leaf extract (C-3) combined with Ginkgo biloba extracts (EGb) showed obvious synergistic effects in a variety of oxidation systems. The aim of the present study was to further confirm the synergy of P. fruticosa combined with EGb viewed from physiological bioavailability and explore the related bioactive mechanism behind the synergism...
December 1, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27904452/virtual-modelling-of-novel-applicator-prototypes-for-cervical-cancer-brachytherapy
#5
Primoz Petric, Robert Hudej, Noora Al-Hammadi, Barbara Segedin
BACKGROUND: Standard applicators for cervical cancer Brachytherapy (BT) do not always achieve acceptable balance between target volume and normal tissue irradiation. We aimed to develop an innovative method of Target-volume Density Mapping (TDM) for modelling of novel applicator prototypes with optimal coverage characteristics. Patients and methods. Development of Contour-Analysis Tool 2 (CAT-2) software for TDM generation was the core priority of our task group. Main requests regarding software functionalities were formulated and guided the coding process...
December 1, 2016: Radiology and Oncology
https://www.readbyqxmd.com/read/27900596/pharmacological-evaluation-for-improvement-of-kanazawa-sutra-medicinal-thread-for-anal-fistula
#6
Takami Yokogawa, Yohei Sasaki, Hirokazu Ando, Katsuya Yamamoto, Masayuki Mikage
Kanazawa Sutra (KanS) is a medicinal thread that is used for the treatment of anal fistula. It is used as a substitute for Kshara Sutra (KS) which is used in Ayurvedic medicine. KanS is composed of Ficus carica latex (FCL), Capsicum annuum tincture (CAT), Achyranthes fauriei Kshara (which is processed ash from the whole plant) and powdered Curcuma longa rhizome (CLR). In this study, we evaluated the ingredients of KanS by measuring nitric oxide (NO) production in murine macrophage-like cell line J774.1 as well as examining cytotoxicity to rat skeletal muscle myoblasts (L6) and L6 differentiation, with a view to improving its pharmacological effect...
November 29, 2016: Journal of Natural Medicines
https://www.readbyqxmd.com/read/27895068/impact-of-a-dedicated-cancer-associated-thrombosis-service-on-clinical-outcomes-a-mixed-methods-evaluation-of-a-clinical-improvement-exercise
#7
Simon Noble, Nikki Pease, Jessica Sui, James Davies, Sarah Lewis, Usman Malik, Raza Alikhan, Hayley Prout, Annmarie Nelson
OBJECTIVES: Cancer-associated thrombosis (CAT) complex condition, which may present to any healthcare professional and at any point during the cancer journey. As such, patients may be managed by a number of specialties, resulting in inconsistent practice and suboptimal care. We describe the development of a dedicated CAT service and its evaluation. SETTING: Specialist cancer centre, district general hospital and primary care. PARTICIPANTS: Patients with CAT and their referring clinicians...
November 28, 2016: BMJ Open
https://www.readbyqxmd.com/read/27859751/subclinical-bacteriuria-in-older-cats-and-its-association-with-survival
#8
J D White, N J Cave, A Grinberg, D G Thomas, C Heuer
BACKGROUND: Bacterial urinary tract infections are uncommon in cats in general but the prevalence increases to 29% in older cats with comorbidities (Veterinary Clinical Pathology 2008, 37, 317; Journal of Feline Medicine & Surgery 2007, 9, 124; Veterinary Microbiology 2009, 136, 130). Frequently, the infections are subclinical. The clinical relevance of subclinical bacteriuria (SB) is uncertain, and the optimal treatment requires clarification. OBJECTIVE: Prospective, observational study to: (i) identify the prevalence and incidence count of SB in older (≥7 years), nonazotemic cats, (ii) evaluate specific risk factors for SB, and (iii) investigate the potential relationship between untreated SB and survival...
November 2016: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27852814/spontaneous-proliferative-and-neoplastic-lesions-in-thyroid-and-parathyroid-glands-of-nondomestic-felids
#9
Jenny P Pope, James Steeil, Edward C Ramsay, Danielle Reel, Shelley J Newman
Based on microscopic and immunohistochemical characterization, we documented spontaneous proliferative and neoplastic lesions in the thyroid and parathyroid glands of nondomestic felids. Ten animals (4 leopards, 3 tigers, and 3 cougars), all with a previous diagnosis of thyroid neoplasia were identified from the University of Tennessee College of Veterinary Medicine database. The mean age of affected animals was 15.9 y. Twelve neoplasms were identified; 2 animals had 2 concurrent neoplasms. After immunohistochemical characterization using a panel of chromogranin A, thyroglobulin, and calcitonin, 7 of the former thyroid neoplasms were diagnosed as thyroid adenomas, 1 was diagnosed as a thyroid carcinoma, and 4 were diagnosed as parathyroid adenomas...
November 15, 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27847530/aqueous-extract-of-clerodendranthus-spicatus-exerts-protective-effect-on-uv-induced-photoaged-mice-skin
#10
Lan Wang, Xie Zhang, Yong-Xian Li, Lie-Qiang Xu, Cai-Lan Li, Zhen-Biao Zhang, Jia-Li Liang, Zi-Ren Su, Hui-Fang Zeng, Yu-Cui Li
Clerodendranthus spicatus (Thunb.) C.Y.Wu (CS) is commonly used to treat kidney diseases in traditional Chinese medicine for its prominent anti-inflammatory effect and nourishing function to kidneys. In this study, aqueous extract of CS was assessed for its protective effect on UV-induced skin damage of mice. The chemical compositions of CS aqueous extract were determined by HPLC-ESI-MS/MS, in which 10 components were identified. During the experimental period, CS (0.9, 1.8, and 3.6 g/mL) was externally applied to shaved dorsal skins of mice prior to UV irradiation, daily for ten weeks...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27829418/hepatoprotective-potential-of-fagonia-olivieri-dc-against-acetaminophen-induced-toxicity-in-rat
#11
Umbreen Rashid, Muhammad Rashid Khan, Moniba Sajid
BACKGROUND: Fagonia olivieri (DC) being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. In this study we have evaluated the F. olivieri whole methanol extract and its derived fractions for various in vitro and in vivo antioxidant studies. METHODS: The crude methanol extract of the whole plant of F. olivieri (FOM) and its derived fractions; n-hexane (FOH), chloroform (FOC), ethyl acetate (FOE), n-butanol (FOB) and aqueous (FOA) were evaluated for the total phenolic and flavonoid content and in vitro antioxidant abilities...
November 9, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27828770/responding-to-a-zoonotic-emergency-with-multi-omics-research-pentatrichomonas-hominis-hydrogenosomal-protein-characterization-with-use-of-rna-sequencing-and-proteomics
#12
Yi-Kai Fang, Kun-Yi Chien, Kuo-Yang Huang, Wei-Hung Cheng, Fu-Mann Ku, Rose Lin, Ting-Wen Chen, Po-Jung Huang, Cheng-Hsun Chiu, Petrus Tang
Pentatrichomonas hominis is an anaerobic flagellated protist that colonizes the large intestine of a number of mammals, including cats, dogs, nonhuman primates, and humans. The wide host range of this organism is alarming and suggests a rising zoonotic emergency. However, knowledge on in-depth biology of this protist is still limited. Similar to the human pathogen, Trichomonas vaginalis, P. hominis possesses hydrogenosomes instead of mitochondria. Studies in T. vaginalis indicated that hydrogenosome is essential for cell survival and associated with numerous pivotal biological functions, including drug resistance...
November 2016: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/27822584/in-vivo-assessment-of-the-antimalarial-and-spleen-protective-activities-of-the-saudi-propolis-methanolic-extract
#13
Qwait AlGabbani, Lamjed Mansour, Yasser A Elnakady, Saleh Al-Quraishy, Suliman Alomar, Esam M Al-Shaebi, Abdel-Azeem S Abdel-Baki
Antimalarial drug resistance is the main therapeutic challenge to the control of the disease, making the search for new compounds as alternative treatments of central importance. Propolis has a long history of medicinal use due to its antifungal, antibacterial and antiprotozoal properties. The present study therefore aimed to evaluate the antimalarial activity of the Saudi propolis methanolic extract against Plasmodium chabaudi infection in mice. To this end, albino mice were divided into five groups: the first group was the normal control; the second, third, fourth and fifth groups were infected intraperitoneally with 10(6) P...
November 7, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27821377/-vigilance-for-veterinary-medicinal-products-reports-of-adverse-reactions-in-the-year-2015
#14
C Müntener, J Kupper, H Naegeli, B Gassner
A total of 292 adverse reactions to veterinary medicinal products were reported during the year 2015. This represents an increase of 9% compared to the previous year (268 reports). Similar to previous years, most of the reactions reported were linked to the use of antiparasitics (55.1%), non-steroidal anti-inflammatory products (8.9%) or antiinfectives (9.3%). The affected animal species were primarily dogs (198 reports) and cats (42 reports), followed by cattle (31 reports) and horses (8 reports). Additional 42 reports were provided within the frame of consultations with Tox Info Suisse in Zürich and involved mainly the excessive intake of flavored tablets...
November 2016: Schweizer Archiv Für Tierheilkunde
https://www.readbyqxmd.com/read/27796436/enhanced-extracellular-production-of-l-asparaginase-from-bacillus-subtilis-168-by-b-subtilis-wb600-through-a-combined-strategy
#15
Yue Feng, Song Liu, Yun Jiao, Hui Gao, Miao Wang, Guocheng Du, Jian Chen
L-asparaginase (EC 3.5.1.1, ASN) exhibits great commercial value due to its uses in the food and medicine industry. In this study, we reported the enhanced expression of type II ASN from Bacillus subtilis 168 in B. subtilis WB600 through a combined strategy. First, eight signal peptides (the signal peptide of the ASN, ywbN, yvgO, amyE, oppA, vpr, lipA, and wapA) were used for ASN secretion in B. subtilis by using Hpa II promoter, respectively. The signal peptide wapA achieved the highest extracellular ASN activity (28...
October 28, 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27794509/effects-of-crude-hydroalcoholic-extract-of-syzygium-cumini-l-skeels-leaves-and-continuous-aerobic-training-in-rats-with-diabetes-induced-by-a-high-fat-diet-and-low-doses-of-streptozotocin
#16
G Baldissera, N D M Sperotto, H T Rosa, J G Henn, V F Peres, D J Moura, R Roehrs, E L G Denardin, P Dal Lago, R B Nunes, J Saffi
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Syzygium cumini (L.) or Skeels (Myrtaceae) are widely used in Brazilian folk medicine to treat diabetes. AIM OF THE STUDY: The present study evaluated the functional capacity, biochemical parameters, oxidative stress and DNA damage from eight weeks of intervention with a crude hydroalcoholic extract of S. cumini leaves (EBH) and continuous aerobic training (TAC) in diabetic (D) rats. MATERIALS AND METHODS: A hydroalcoholic (50%) extract was prepared by ultrasound and phytochemical parameters (total phenols, total tannins and myricetin content) were analyzed...
October 26, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27766257/prevalence-and-risk-factors-of-colonization-with-staphylococcus-aureus-in-healthy-pet-cats-kept-in-the-city-households
#17
Karolina Bierowiec, Katarzyna Płoneczka-Janeczko, Krzysztof Rypuła
Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA), is a significant pathogen in both human medicine and veterinary medicine. The importance of pets as reservoirs of human infections is still poorly understood. This article provides detailed information of a cross-sectional study of a S. aureus colonization in clinically healthy indoor cats. The study systematically assessed a number of different anatomical locations for the S. aureus colonization and the influence of a range of potential risk factors on the value of the final S...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27755584/molecular-characterization-of-the-cytidine-monophosphate-n-acetylneuraminic-acid-hydroxylase-cmah-gene-associated-with-the-feline-ab-blood-group-system
#18
Toshinori Omi, Shota Nakazawa, Chihiro Udagawa, Naomi Tada, Kazuhiko Ochiai, Yong Hwa Chong, Yuiko Kato, Hiroko Mitsui, Azusa Gin, Hitomi Oda, Daigo Azakami, Kyoichi Tamura, Toshinori Sako, Takeshi Inagaki, Atsushi Sakamoto, Toshihiko Tsutsui, Makoto Bonkobara, Shuichi Tsuchida, Shigenori Ikemoto
Cat's AB blood group system (blood types A, B, and AB) is of major importance in feline transfusion medicine. Type A and type B antigens are Neu5Gc and Neu5Ac, respectively, and the enzyme CMAH participating in the synthesis of Neu5Gc from Neu5Ac is associated with this cat blood group system. Rare type AB erythrocytes express both Neu5Gc and Neu5Ac. Cat serum contains naturally occurring antibodies against antigens occurring in the other blood types. To understand the molecular genetic basis of this blood group system, we investigated the distribution of AB blood group antigens, CMAH gene structure, mutation, diplotypes, and haplotypes of the cat CMAH genes...
2016: PloS One
https://www.readbyqxmd.com/read/27736928/evaluation-of-feline-renal-perfusion-with-contrast-enhanced-ultrasonography-and-scintigraphy
#19
Emmelie Stock, Katrien Vanderperren, Tim Bosmans, André Dobbeleir, Luc Duchateau, Myriam Hesta, Lien Lybaert, Kathelijne Peremans, Eva Vandermeulen, Jimmy Saunders
Contrast-enhanced ultrasound (CEUS) is an emerging technique to evaluate tissue perfusion. Promising results have been obtained in the evaluation of renal perfusion in health and disease, both in human and veterinary medicine. Renal scintigraphy using 99mTc-Mercaptoacetyltriglycine (MAG3) is another non-invasive technique that can be used to evaluate renal perfusion. However, no data are available on the ability of CEUS or 99mTc- MAG3 scintigraphy to detect small changes in renal perfusion in cats. Therefore, both techniques were applied in a normal feline population to evaluate detection possibilities of perfusion changes by angiotensin II (AT II)...
2016: PloS One
https://www.readbyqxmd.com/read/27704053/divergent-isoprenoid-biosynthesis-pathways-in-staphylococcus-species-constitute-a-drug-target-for-treating-infections-in-companion-animals
#20
Ana M Misic, Christine L Cain, Daniel O Morris, Shelley C Rankin, Daniel P Beiting
Staphylococcus species are a leading cause of skin and soft tissue infections in humans and animals, and the antibiotics used to treat these infections are often the same. Methicillin- and multidrug-resistant staphylococcal infections are becoming more common in human and veterinary medicine. From a "One Health" perspective, this overlap in antibiotic use and resistance raises concerns over the potential spread of antibiotic resistance genes. Whole-genome sequencing and comparative genomics analysis revealed that Staphylococcus species use divergent pathways to synthesize isoprenoids...
September 2016: MSphere
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