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https://www.readbyqxmd.com/read/29353439/transgenesis-by-microparticle-bombardment-for-live-imaging-of-fluorescent-proteins-in-pristionchus-pacificus-germline-and-early-embryos
#1
Satoshi Namai, Asako Sugimoto
Pristionchus pacificus is a free-living nematode used as a model organism for evolutionary developmental and ecological biology. Although a transgenic technique to form complex arrays by microinjection has been established in P. pacificus, transgene expression from the array in the germline and early embryos tends to be silenced. Here, we established a method to integrate transgenes into the genome of P. pacificus using microparticle bombardment with hygromycin B selection. Additionally, we isolated a mutant exhibiting significantly lower autofluorescence in the germline and early embryos, facilitating visualization of transgene-derived fluorescent proteins for live imaging...
January 20, 2018: Development Genes and Evolution
https://www.readbyqxmd.com/read/29353409/16s-rrna-gene-sequencing-reveals-altered-composition-of-gut-microbiota-in-individuals-with-kidney-stones
#2
Ruiqiang Tang, Yonghua Jiang, Aihua Tan, Juan Ye, Xiaoying Xian, Yuanliang Xie, Qiuyan Wang, Ziting Yao, Zengnan Mo
Nephrolithiasis is a common urological disease with high prevalence and recurrence rates. Characterizing gut microbiome profiles of nephrolithiasis patients may provide valuable insights and potential biomarkers for the disease. Therefore, we explored the relation between gut microbiome and nephrolithiasis using 16S ribosomal RNA (rRNA) gene sequencing. 13 patients with multiple kidney stones and 13 matched healthy controls were recruited. A decreasing trend in number of observed species in nephrolithiasis patients was detected, although statistical significance was not reached (p = 0...
January 20, 2018: Urolithiasis
https://www.readbyqxmd.com/read/29353246/a-concept-mapping-approach-to-identifying-the-barriers-to-implementing-an-evidence-based-sports-injury-prevention-programme
#3
Alex Donaldson, Aisling Callaghan, Mario Bizzini, Andrew Jowett, Patrick Keyzer, Matthew Nicholson
BACKGROUND AND AIM: Understanding the barriers to programme use is important to facilitate implementation of injury prevention programmes in real-word settings. This study investigated the barriers to coaches of adolescent female soccer teams, in Victoria, Australia, implementing the evidence-based FIFA 11+ injury prevention programme. METHODS: Concept mapping with data collected from 19 soccer coaches and administrators. RESULTS: Brainstorming generated 65 statements as barriers to 11+ implementation...
January 20, 2018: Injury Prevention: Journal of the International Society for Child and Adolescent Injury Prevention
https://www.readbyqxmd.com/read/29353216/an-efficient-swarm-intelligence-approach-to-feature-selection-based-on-invasive-weed-optimization-application-to-multivariate-calibration-and-classification-using-spectroscopic-data
#4
Saheleh Sheykhizadeh, Abdolhossein Naseri
Variable selection plays a key role in classification and multivariate calibration. Variable selection methods are aimed at choosing a set of variables, from a large pool of available predictors, relevant to the analyte concentrations estimation, or to achieve better classification results. Many variable selection techniques have now been introduced among which, those which are based on the methodologies of swarm intelligence optimization have been more respected during a few last decades since they are mainly inspired by nature...
January 12, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29353173/use-of-cu-zn-superoxide-dismutase-tool-for-biomonitoring-marine-environment-pollution-in-the-persian-gulf-and-the-gulf-of-oman
#5
Farrokhzad Zeinali, Ahmad Homaei, Ehsan Kamrani, Seema Patel
Superoxide dismutase (SOD) is the pivotal antioxidant enzyme that defends organisms against the oxidative stresses of superoxide radicals. In this experimental study, purification of SOD from the leaves of Avicennia marina (grey mangrove or white mangrove) from the family Acanthaceae, located in Sirik mangrove forest on the shore of the Gulf of Oman was performed, for the intended characterization of SOD. The Sirik AmSOD (A. marina SOD) expressed optimum activity in the pH range of 6-9 with the maximum activity at pH 8...
January 16, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353171/microcystin-lr-nanobody-screening-from-an-alpaca-phage-display-nanobody-library-and-its-expression-and-application
#6
Chongxin Xu, Ying Yang, Liwen Liu, Jianhong Li, Xiaoqin Liu, Xiao Zhang, Yuan Liu, Cunzheng Zhang, Xianjin Liu
Microcystin-LR (MC-LR) is a type of biotoxin that pollutes the ecological environment and food. The study aimed to obtain new nanobodies from phage nanobody library for determination of MC-LR. The toxin was conjugated to keyhole limpet haemocyanin (KLH) and bovine serum albumin (BSA), respectively, then the conjugates were used as coated antigens for enrichment (coated MC-LR-KLH) and screening (coated MC-LR-BSA) of MC-LR phage nanobodies from an alpaca phage display nanobody library. The antigen-specific phage particles were enriched effectively with four rounds of biopanning...
January 17, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29353015/proteomic-and-functional-variation-within-black-snake-venoms-elapidae-pseudechis
#7
Jonathan Goldenberg, Vittoria Cipriani, Timothy N W Jackson, Kevin Arbuckle, Jordan Debono, Daniel Dashevsky, Nadya Panagides, Maria P Ikonomopoulou, Ivan Koludarov, Bin Li, Renan Castro Santana, Amanda Nouwens, Alun Jones, Chris Hay, Nathan Dunstan, Luke Allen, Brian Bush, John J Miles, Lilin Ge, Hang Fai Kwok, Bryan G Fry
Pseudechis (black snakes) is an Australasian elapid snake genus that inhabits much of mainland Australia, with two representatives confined to Papua New Guinea. The present study is the first to analyse the venom of all 9 described Pseudechis species (plus one undescribed species) to investigate the evolution of venom composition and functional activity. Proteomic results demonstrated that the typical Pseudechis venom profile is dominated by phospholipase A2 toxins. Strong cytotoxicity was the dominant function for most species...
January 15, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29352820/concordance-and-predictors-of-concordance-of-children-s-dietary-intake-as-reported-via-ecological-momentary-assessment-and-24-h-recall
#8
Sydney G O'Connor, Wangjing Ke, Eldin Dzubur, Susan Schembre, Genevieve F Dunton
OBJECTIVE: To provide preliminary evidence in support of using ecological momentary assessment (EMA), a real-time data capture method involving repeated assessments, to measure dietary intake in children by examining the concordance of children's dietary reports through EMA and 24 h recall. DESIGN: Children completed eight days of EMA surveys, reporting on recent dietary intake of four pre-specified food categories ('Fruits or Vegetables', 'Chips or Fries', 'Pastries or Sweets', 'Soda or Energy Drinks'), and completed two 24 h recalls during the same period...
January 21, 2018: Public Health Nutrition
https://www.readbyqxmd.com/read/29352679/inter-sexual-habitat-and-isotopic-niche-segregation-of-the-endangered-monteiro-s-storm-petrel-during-breeding
#9
V H Paiva, J A Ramos, C Nava, V Neves, J Bried, M Magalhães
At-sea distribution and trophic ecology of small seabird species (i.e.<100 g) is far less known when compared to their larger relatives. We studied the habitat use (spatial ecology) and isotopic niches (trophic ecology) of the endangered Monteiro's storm-petrel Hydrobates monteiroi during the incubation and chick-rearing periods of 2013. There was a sexual foraging segregation of Monteiro's storm-petrels during the breeding period (tracking data) but also during the non-breeding stage (stable isotope analysis)...
December 30, 2017: Zoology: Analysis of Complex Systems, ZACS
https://www.readbyqxmd.com/read/29352539/income-inequality-and-depression-a-systematic-review-and-meta-analysis-of-the-association-and-a-scoping-review-of-mechanisms
#10
Vikram Patel, Jonathan K Burns, Monisha Dhingra, Leslie Tarver, Brandon A Kohrt, Crick Lund
Most countries have witnessed a dramatic increase of income inequality in the past three decades. This paper addresses the question of whether income inequality is associated with the population prevalence of depression and, if so, the potential mechanisms and pathways which may explain this association. Our systematic review included 26 studies, mostly from high-income countries. Nearly two-thirds of all studies and five out of six longitudinal studies reported a statistically significant positive relationship between income inequality and risk of depression; only one study reported a statistically significant negative relationship...
February 2018: World Psychiatry: Official Journal of the World Psychiatric Association (WPA)
https://www.readbyqxmd.com/read/29352480/temporal-heterogeneity-increases-with-spatial-heterogeneity-in-ecological-communities
#11
Scott L Collins, Meghan L Avolio, Corinna Gries, Lauren M Hallett, Sally E Koerner, Kimberly J La Pierre, Andrew L Rypel, Eric R Sokol, Samuel B Fey, Dan F B Flynn, Sydney K Jones, Laura M Ladwig, Julie Ripplinger, Matt B Jones
Heterogeneity is increasingly recognized as a foundational characteristic of ecological systems. Under global change, understanding temporal community heterogeneity is necessary for predicting the stability of ecosystem functions and services. Indeed, spatial heterogeneity is commonly used in alternative stable state theory as a predictor of temporal heterogeneity and therefore an early indicator of regime shifts. To evaluate whether spatial heterogeneity in species composition is predictive of temporal heterogeneity in ecological communities, we analyzed 68 community datasets spanning freshwater and terrestrial systems where measures of species abundance were replicated over space and time...
January 20, 2018: Ecology
https://www.readbyqxmd.com/read/29352197/phylogenetic-and-functional-structure-of-wintering-waterbird-communities-associated-with-ecological-differences
#12
Xianli Che, Min Zhang, Yanyan Zhao, Qiang Zhang, Qing Quan, Anders Møller, Fasheng Zou
Ecological differences may be related to community component divisions between Oriental (west) and Sino-Japanese (east) realms, and such differences may result in weak geographical breaks in migratory species that are highly mobile. Here, we conducted comparative phylogenetic and functional structure analyses of wintering waterbird communities in southern China across two realms and subsequently examined possible climate drivers of the observed patterns. An analysis based on such highly migratory species is particularly telling because migration is bound to reduce or completely eliminate any divergence between communities...
January 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29351889/disentangling-the-effects-of-low-ph-and-metal-mixture-toxicity-on-macroinvertebrate-diversity
#13
Riccardo Fornaroli, Alessio Ippolito, Mari J Tolkkinen, Heikki Mykrä, Timo Muotka, Laurie S Balistrieri, Travis S Schmidt
One of the primary goals of biological assessment of streams is to identify which of a suite of chemical stressors is limiting their ecological potential. Elevated metal concentrations in streams are often associated with low pH, yet the effects of these two potentially limiting factors of freshwater biodiversity are rarely considered to interact beyond the effects of pH on metal speciation. Using a dataset from two continents, a biogeochemical model of the toxicity of metal mixtures (Al, Cd, Cu, Pb, Zn) and quantile regression, we addressed the relative importance of both pH and metals as limiting factors for macroinvertebrate communities...
January 16, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29351736/studying-medical-communication-with-video-vignettes-a-randomized-study-on-how-variations-in-video-vignette-introduction-format-and-camera-focus-influence-analogue-patients-engagement
#14
Leonie N C Visser, Nadine Bol, Marij A Hillen, Mathilde G E Verdam, Hanneke C J M de Haes, Julia C M van Weert, Ellen M A Smets
BACKGROUND: Video vignettes are used to test the effects of physicians' communication on patient outcomes. Methodological choices in video-vignette development may have far-stretching consequences for participants' engagement with the video, and thus the ecological validity of this design. To supplement the scant evidence in this field, this study tested how variations in video-vignette introduction format and camera focus influence participants' engagement with a video vignette showing a bad news consultation...
January 19, 2018: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/29351591/could-seasonally-deteriorating-environments-favour-the-evolution-of-autogamous-selfing-and-a-drought-escape-physiology-through-indirect-selection-a-test-of-the-time-limitation-hypothesis-using-artificial-selection-in-clarkia
#15
Simon K Emms, Alisa A Hove, Leah S Dudley, Susan J Mazer, Amy S Verhoeven
Background and Aims: The evolution of selfing from outcrossing may be the most common transition in plant reproductive systems and is associated with a variety of ecological circumstances and life history strategies. The most widely discussed explanation for these associations is the reproductive assurance hypothesis - the proposition that selfing is favoured because it increases female fitness when outcross pollen receipt is limited. Here an alternative explanation, the time limitation hypothesis, is addressed, one scenario of which proposes that selfing may evolve as a correlated response to selection for a faster life cycle in seasonally deteriorating environments...
January 17, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29351330/use-of-influenza-antivirals-in-patients-hospitalized-in-hong-kong-2000-2015
#16
Benjamin J Cowling, Celine S L Chui, Wey Wen Lim, Peng Wu, Christopher K M Hui, J S Malik Peiris, Esther W Chan
OBJECTIVES: We aimed to describe patterns in the usage of antivirals to treat influenza virus infection in hospitals in Hong Kong from 2000 through 2015. METHODS: We analyzed centralized electronic health records that included dispensation information and diagnosis codes. Information collected on admissions included patient age, sex, admission year and month, and medications dispensed, and were matched with the first 15 discharge diagnosis codes. We divided monthly admission episodes by relevant population denominators to obtain admission rates, and stratified analyses by drug type, age group, and diagnosis codes...
2018: PloS One
https://www.readbyqxmd.com/read/29351302/extension-of-the-viral-ecology-in-humans-using-viral-profile-hidden-markov-models
#17
Zurab Bzhalava, Emilie Hultin, Joakim Dillner
When human samples are sequenced, many assembled contigs are "unknown", as conventional alignments find no similarity to known sequences. Hidden Markov models (HMM) exploit the positions of specific nucleotides in protein-encoding codons in various microbes. The algorithm HMMER3 implements HMM using a reference set of sequences encoding viral proteins, "vFam". We used HMMER3 analysis of "unknown" human sample-derived sequences and identified 510 contigs distantly related to viruses (Anelloviridae (n = 1), Baculoviridae (n = 34), Circoviridae (n = 35), Caulimoviridae (n = 3), Closteroviridae (n = 5), Geminiviridae (n = 21), Herpesviridae (n = 10), Iridoviridae (n = 12), Marseillevirus (n = 26), Mimiviridae (n = 80), Phycodnaviridae (n = 165), Poxviridae (n = 23), Retroviridae (n = 6) and 89 contigs related to described viruses not yet assigned to any taxonomic family)...
2018: PloS One
https://www.readbyqxmd.com/read/29351290/land-use-change-in-oil-palm-dominated-tropical-landscapes-an-agent-based-model-to-explore-ecological-and-socio-economic-trade-offs
#18
Claudia Dislich, Elisabeth Hettig, Jan Salecker, Johannes Heinonen, Jann Lay, Katrin M Meyer, Kerstin Wiegand, Suria Tarigan
Land-use changes have dramatically transformed tropical landscapes. We describe an ecological-economic land-use change model as an integrated, exploratory tool used to analyze how tropical land-use change affects ecological and socio-economic functions. The model analysis seeks to determine what kind of landscape mosaic can improve the ensemble of ecosystem functioning, biodiversity, and economic benefit based on the synergies and trade-offs that we have to account for. More specifically, (1) how do specific ecosystem functions, such as carbon storage, and economic functions, such as household consumption, relate to each other? (2) How do external factors, such as the output prices of crops, affect these relationships? (3) How do these relationships change when production inefficiency differs between smallholder farmers and learning is incorporated? We initialize the ecological-economic model with artificially generated land-use maps parameterized to our study region...
2018: PloS One
https://www.readbyqxmd.com/read/29351219/investigating-binge-eating-using-ecological-momentary-assessment-the-importance-of-an-appropriate-sampling-frequency
#19
Tobias D Kockler, Philip S Santangelo, Ulrich W Ebner-Priemer
With great interest, we read the recently published review on emotion regulation in binge eating disorder (BED) by Dingemans et al. [1].[...].
January 19, 2018: Nutrients
https://www.readbyqxmd.com/read/29351020/a-dynamic-state-model-of-migratory-behavior-and-physiology-to-assess-the-consequences-of-environmental-variation-and-anthropogenic-disturbance-on-marine-vertebrates
#20
Enrico Pirotta, Marc Mangel, Daniel P Costa, Bruce Mate, Jeremy A Goldbogen, Daniel M Palacios, Luis A Hückstädt, Elizabeth A McHuron, Lisa Schwarz, Leslie New
Integrating behavior and physiology is critical to formulating new hypotheses on the evolution of animal life-history strategies. Migratory capital breeders acquire most of the energy they need to sustain migration, gestation, and lactation before parturition. Therefore, when predicting the impact of environmental variation on such species, a mechanistic understanding of the physiology of their migratory behavior is required. Using baleen whales as a model system, we developed a dynamic state variable model that captures the interplay among behavioral decisions, energy, reproductive needs, and the environment...
February 2018: American Naturalist
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