Lien Dejager, Sophia Banton, Patricia Marques, Gabriela Rinikova, Sabrina Lory, Elizabeth S Hickford, Carole Martin-Hamka, Mark Penney, Sasker Grootjans
Ligand-binding assays (LBAs) rely on the reversible, noncovalent binding between the analyte of interest and the assay reagents, and understanding their dynamic equilibrium is key to building robust LBA methods. Although the dynamic interplay of free and bound fractions can be calculated using mathematical models, these are not routinely applied. This approach is costly in terms of both assay development time and reagents, and can result in an under-exploration of the possible parameter combinations. Therefore, we have created a user-friendly simulation tool to facilitate LBA development (the BiSim Tool)...
April 17, 2024: Bioanalysis