Jesper V Olsen, Lyris M F de Godoy, Guoqing Li, Boris Macek, Peter Mortensen, Reinhold Pesch, Alexander Makarov, Oliver Lange, Stevan Horning, Matthias Mann
Mass accuracy is a key parameter of mass spectrometric performance. TOF instruments can reach low parts per million, and FT-ICR instruments are capable of even greater accuracy provided ion numbers are well controlled. Here we demonstrate sub-ppm mass accuracy on a linear ion trap coupled via a radio frequency-only storage trap (C-trap) to the orbitrap mass spectrometer (LTQ Orbitrap). Prior to acquisition of a spectrum, a background ion originating from ambient air is first transferred to the C-trap. Ions forming the MS or MS(n) spectrum are then added to this species, and all ions are injected into the orbitrap for analysis...
December 2005: Molecular & Cellular Proteomics: MCP