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https://www.readbyqxmd.com/read/28319828/genistein-affects-proliferation-and-migration-of-bovine-oviductal-epithelial-cells
#1
Daniela C García, Pablo A Valdecantos, Dora C Miceli, Mariela Roldán-Olarte
Genistein is one of the most abundant isoflavones in soybean. This molecule induces cell cycle arrest and apoptosis in different normal and cancer cells. Genistein has been of considerable interest due to its adverse effects on bovine reproduction, altering estrous cycle, implantation and fetal development and producing subfertility or infertility. The objective of this work was to study the effects of genistein on the expression of selected genes involved in the regulation of cell cycle and apoptosis. Primary cultures of bovine oviductal epithelial cells (BOEC) were treated with different genistein concentrations (0...
March 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28258156/maternal-metabolic-stress-may-affect-oviduct-gatekeeper-function
#2
Lies Jordaens, Veerle Van Hoeck, Veronica Maillo, Alfonso Gutierrez-Adan, Waleed Fawzy A Marei, Bruno Vlaeminck, Sofie Thys, Roger G S Sturmey, Peter Bols, Jo Leroy
We hypothesized that elevated non-esterified fatty acids (NEFA) modify in vitro bovine oviduct epithelial cell (BOEC) metabolism and barrier function. Hereto, BOECs were studied in a polarized system with 24h-treatments at day 9: 1) CONTROL (0µM NEFA + 0%EtOH), 2) SOLVENT CONTROL (0µM NEFA + 0.45%EtOH), 3) BASAL NEFA (720µM NEFA + 0.45%EtOH in the basal compartment), 4) APICAL NEFA (720µM NEFA + 0.45%EtOH in the apical compartment). FITC-albumin was used for monolayer permeability assessment, and related to Transepithelial Electric Resistance (TER)...
March 3, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28250237/bovine-embryo-oviduct-interaction-in-vitro-reveals-an-early-crosstalk-mediated-by-bmp-signaling
#3
Elina Vanesa García, Meriem Hamdi, Antonio Daniel Barrera, Maria-Jesús Sánchez Calabuig, Alfonso Gutierrez-Adan, Dimitrios Rizos
Signaling components of Bone Morphogenetic Proteins (BMPs) are expressed in an anatomically and temporally regulated fashion in bovine oviduct. However, a local response of this signaling to the presence of the embryo has yet to be elucidated. The aim of the present study was to evaluate if early embryo-oviduct interaction induces changes in the gene expression of BMP signaling components. For this purpose, we used an in vitro co-culture system to investigate the local interaction between bovine oviductal epithelial cells (BOEC) from the isthmus region with early embryos during two developmental periods: before (from the 2-cell to 8-cell stage) or during (from the 8-cell to 16-cell stage) the main phase of embryonic genome activation (EGA)...
March 1, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28194463/improved-bovine-embryo-production-in-an-oviduct-on-a-chip-system-prevention-of-poly-spermic-fertilization-and-parthenogenic-activation
#4
Marcia A M M Ferraz, Heiko H W Henning, Pedro F Costa, Jos Malda, Ferry P Melchels, R Wubbolts, Tom A E Stout, Peter L A M Vos, Bart M Gadella
The oviduct provides the natural micro-environment for gamete interaction, fertilization and early embryo development in mammals, such as the cow. In conventional culture systems, bovine oviduct epithelial cells (BOEC) undergo a rapid loss of essential differentiated cell properties; we aimed to develop a more physiological in vitro oviduct culture system capable of supporting fertilization. U-shaped chambers were produced using stereo-lithography and mounted with polycarbonate membranes, which were used as culture inserts for primary BOECs...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/27662642/sperm-binding-to-oviduct-epithelial-cells-enhances-tgfb1-and-il10-expressions-in-epithelial-cells-as-well-as-neutrophils-in-vitro-prostaglandin-e2-as-a-main-regulator-of-anti-inflammatory-response-in-the-bovine-oviduct
#5
Mohamed Samy Yousef, Mohamed Ali Marey, Nina Hambruch, Hiroyuki Hayakawa, Takashi Shimizu, Hassan Ali Hussien, Abdel-Razek Khalifa Abdel-Razek, Christiane Pfarrer, Akio Miyamoto
Sperm are allogenic to the female genital tract; however, oviducts provide optimal conditions for survival and capacitation of these non-self cells until fertilization. Recently, we showed that oviduct-conditioned media and prostaglandin E2 (PGE2) suppress sperm phagocytosis by polymorphonuclear neutrophils (PMNs) under physiological conditions. We hypothesized that sperm binding to bovine oviduct epithelial cells (BOECs) could change the local innate immunity via PGE2. As the first step to obtain basic information, sub-confluent BOEC monolayers were co-cultured with swim-up sperm for 2 h...
2016: PloS One
https://www.readbyqxmd.com/read/27543438/intron-retention-resulting-from-a-silent-mutation-in-the-vwf-gene-that-structurally-influences-the-5-splice-site
#6
Hamideh Yadegari, Arijit Biswas, Mohammad Suhail Akhter, Julia Driesen, Vytautas Ivaskevicius, Natascha Marquardt, Johannes Oldenburg
Disease-associated silent mutations are considered to affect the accurate pre-messenger RNA (mRNA) splicing either by influencing regulatory elements, leading to exon skipping, or by creating a new cryptic splice site. This study describes a new molecular pathological mechanism by which a silent mutation inhibits splicing and leads to intron retention. We identified a heterozygous silent mutation, c.7464C>T, in exon 44 of the von Willebrand factor (VWF) gene in a family with type 1 von Willebrand disease...
October 27, 2016: Blood
https://www.readbyqxmd.com/read/27526775/mrna-expression-pattern-of-selected-candidate-genes-differs-in-bovine-oviductal-epithelial-cells-in-vitro-compared-with-the-in-vivo-state-and-during-cell-culture-passages
#7
COMPARATIVE STUDY
Sadjad Danesh Mesgaran, Jutta Sharbati, Ralf Einspanier, Christoph Gabler
BACKGROUND: The mammalian oviduct provides the optimal environment for gamete maturation including sperm capacitation, fertilization, and development of the early embryo. Various cell culture models for primary bovine oviductal epithelial cells (BOEC) were established to reveal such physiological events. The aim of this study was to evaluate 17 candidate mRNA expression patterns in oviductal epithelial cells (1) in transition from in vivo cells to in vitro cells; (2) during three consecutive cell culture passages; (3) affected by the impact of LOW or HIGH glucose content media; and (4) influenced by different phases of the estrous cycle in vivo and in vitro...
August 15, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27345318/local-immune-system-in-oviduct-physiology-and-pathophysiology-attack-or-tolerance
#8
REVIEW
M A Marey, M S Yousef, R Kowsar, N Hambruch, T Shimizu, C Pfarrer, A Miyamoto
The local immune system in the oviduct has a unique ability to deal with pathogens, allogeneic spermatozoa, and the semi-allogeneic embryo. To achieve this, it seems likely that the oviduct possesses an efficient and strictly controlled immune system that maintains optimal conditions for fertilization and early embryo development. The presence of a proper sperm and/or embryo-oviduct interaction begs the question of whether the local immune system in the oviduct exerts beneficial or deleterious effects on sperm and early embryo; support or attack?...
July 2016: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/27317792/characterization-of-aberrant-splicing-of-von-willebrand-factor-in-von-willebrand-disease-an-underrecognized-mechanism
#9
Lindsey Hawke, Mackenzie L Bowman, Man-Chiu Poon, Mary-Frances Scully, Georges-Etienne Rivard, Paula D James
Approximately 10% of von Willebrand factor (VWF) gene mutations are thought to alter messenger RNA (mRNA) splicing through disruption of consensus splice sites. This mechanism is likely underrecognized and affected by mutations outside consensus splice sites. During VWF synthesis, splicing abnormalities lead to qualitative defects or quantitative deficiencies in VWF. This study investigated the pathologic mechanism acting in 3 von Willebrand disease (VWD) families with putative splicing mutations using patient-derived blood outgrowth endothelial cells (BOECs) and a heterologous human embryonic kidney (HEK 293(T)) cell model...
July 28, 2016: Blood
https://www.readbyqxmd.com/read/27236750/von-willebrand-factor-regulates-complement-on-endothelial-cells
#10
Damien G Noone, Magdalena Riedl, Fred G Pluthero, Mackenzie L Bowman, M Kathryn Liszewski, Lily Lu, Yi Quan, Steve Balgobin, Reinhard Schneppenheim, Sonja Schneppenheim, Ulrich Budde, Paula James, John P Atkinson, Nades Palaniyar, Walter H A Kahr, Christoph Licht
Atypical hemolytic uremic syndrome and thrombotic thrombocytopenic purpura have traditionally been considered separate entities. Defects in the regulation of the complement alternative pathway occur in atypical hemolytic uremic syndrome, and defects in the cleavage of von Willebrand factor (VWF)-multimers arise in thrombotic thrombocytopenic purpura. However, recent studies suggest that both entities are related as defects in the disease-causing pathways overlap or show functional interactions. Here we investigate the possible functional link of VWF-multimers and the complement system on endothelial cells...
July 2016: Kidney International
https://www.readbyqxmd.com/read/27132094/short-communication-urea-induces-t-helper-2-th2-type-environment-at-transcriptional-level-and-prostaglandin-e2-secretion-in-bovine-oviduct-epithelial-cells-in-culture
#11
R Kowsar, M A Marey, T Shimizu, A Miyamoto
Excess dietary protein intake in early lactation dairy cows resulting in blood urea nitrogen of greater than 19 to 20mg/dL is associated with decreased fertility. Little is known about the local interference of urea in the normal immunological environment of the oviduct that provides optimal conditions for early reproductive events. A bovine oviduct epithelial cell (BOEC) culture was used to determine how urea influences immune environment. The BOEC monolayer was supplemented with low (20mg/dL) and high (40mg/dL) concentrations of urea together with ovarian steroids, estradiol (1ng/mL) and progesterone (1ng/mL), and LH (10ng/mL) at concentrations observed during the preovulatory period...
July 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27091182/neovascularization-potential-of-blood-outgrowth-endothelial-cells-from-patients-with-stable-ischemic-heart-failure-is-preserved
#12
Dieter Dauwe, Beatriz Pelacho, Arief Wibowo, Ann-Sophie Walravens, Kristoff Verdonck, Hilde Gillijns, Ellen Caluwe, Peter Pokreisz, Nick van Gastel, Geert Carmeliet, Maarten Depypere, Frederik Maes, Nina Vanden Driessche, Walter Droogne, Johan Van Cleemput, Johan Vanhaecke, Felipe Prosper, Catherine Verfaillie, Aernout Luttun, Stefan Janssens
BACKGROUND: Blood outgrowth endothelial cells (BOECs) mediate therapeutic neovascularization in experimental models, but outgrowth characteristics and functionality of BOECs from patients with ischemic cardiomyopathy (ICMP) are unknown. We compared outgrowth efficiency and in vitro and in vivo functionality of BOECs derived from ICMP with BOECs from age-matched (ACON) and healthy young (CON) controls. METHODS AND RESULTS: We isolated 3.6±0.6 BOEC colonies/100×10(6) mononuclear cells (MNCs) from 60-mL blood samples of ICMP patients (n=45; age: 66±1 years; LVEF: 31±2%) versus 3...
April 18, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/26845570/extracellular-vesicles-from-boec-in-in-vitro-embryo-development-and-quality
#13
Ricaurte Lopera-Vásquez, Meriem Hamdi, Beatriz Fernandez-Fuertes, Verónica Maillo, Paula Beltrán-Breña, Alexandra Calle, Alberto Redruello, Soraya López-Martín, Alfonso Gutierrez-Adán, María Yañez-Mó, Miguel Ángel Ramirez, Dimitrios Rizos
To evaluate the effect of conditioned media (CM) and Extracellular Vesicles (EVs) derived from bovine oviduct epithelial cell (BOEC) lines on the developmental capacity of bovine zygotes and the quality of embryos produced in vitro, presumptive zygotes were cultured under specific conditions. In experiment 1, zygotes were cultured either on monolayers from BOEC extended culture (E), together with fresh BOEC suspension cells, or with BOEC-CM from fresh or E-monolayers. In experiment 2, EVs were isolated from BOEC-CM and characterized (150-200 nm) by Nanosight® and electron microscopy...
2016: PloS One
https://www.readbyqxmd.com/read/26781611/endothelin-1-downregulates-sperm-phagocytosis-by-neutrophils-in-vitro-a-physiological-implication-in-bovine-oviduct-immunity
#14
Mohamed Ali Marey, Mohamed Samy Yousef, Jinghui Liu, Kazuhiro Morita, Motoki Sasaki, Hiroyuki Hayakawa, Takashi Shimizu, Ibrahim I Elshahawy, Akio Miyamoto
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro...
April 22, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/26302033/bovine-oviduct-epithelial-cells-dedifferentiate-partly-in-culture-while-maintaining-their-ability-to-improve-early-embryo-development-rate-and-quality
#15
B Schmaltz-Panneau, Y Locatelli, S Uzbekova, C Perreau, P Mermillod
There are convincing arguments to suggest that the success of early reproductive events is reliant on a satisfactory dialogue between gametes-embryo and the oviduct epithelium. The aim of this study was to develop and characterize an in vitro model to study these interactions. Cattle zygotes produced in vitro were cultured in either SOF or TCM-199 in the presence or absence of bovine oviduct cell monolayers (BOEC), under 20% or 5% O2 . The embryonic development rate and its quality (cell numbers, cryosurvival) were evaluated, as were the BOEC contents in 11 candidate transcripts (real-time PCR) at different time points...
October 2015: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/26270243/angiogenic-characteristics-of-blood-outgrowth-endothelial-cells-from-patients-with-von-willebrand-disease
#16
COMPARATIVE STUDY
D J Groeneveld, T van Bekkum, R J Dirven, J-W Wang, J Voorberg, P H Reitsma, J Eikenboom
BACKGROUND: Endothelial von Willebrand factor (VWF) inhibits angiogenesis. Accordingly, blood outgrowth endothelial cells (BOECs) isolated from von Willebrand disease (VWD) patients showed enhanced in vitro angiogenesis when compared with healthy control BOECs. Characterization of the angiogenic response of VWD BOECs is limited and differences between the different types of VWD have not been investigated in detail. OBJECTIVES: The aim of this study was to further explore the potential pathogenic effect of VWF mutations on angiogenesis...
October 2015: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/26176954/endothelial-expression-of-endothelin-receptor-a-in-the-systemic-capillary-leak-syndrome
#17
Albert C Sek, Zhihui Xie, Kaoru Terai, Lauren M Long, Celeste Nelson, Arkadiusz Z Dudek, Kirk M Druey
Idiopathic systemic capillary leak syndrome (SCLS) is a rare and potentially fatal vascular disorder characterized by reversible bouts of hypotension and edema resulting from fluid and solute escape into soft tissues. Although spikes in permeability-inducing factors have been linked to acute SCLS flares, whether or not they act on an inherently dysfunctional endothelium is unknown. To assess the contribution of endothelial-intrinsic mechanisms in SCLS, we derived blood-outgrowth endothelial cells (BOEC) from patients and healthy controls and examined gene expression patterns...
2015: PloS One
https://www.readbyqxmd.com/read/26167008/first-drug-treatment-failures-in-42-turkish-children-with-idiopathic-childhood-occipital-epilepsies
#18
Faruk Incecik, Ozlem M Herguner, Sakir Altunbasak
BACKGROUND: The early and late benign occipital epilepsies of childhood (BOEC) are described as two discrete electro-clinical syndromes, eponymously known as Panayiotopoulos and Gastaut syndromes. The purpose of this study was to identify predictors of failure to respond to the initial antiepileptic drug (AED). MATERIALS AND METHODS: A total of 42 children with BOEC were enrolled. Predictive factors were analyzed by survival methods. RESULTS: Among the 42, 25 patients (59...
July 2015: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/26119475/elevated-non-esterified-fatty-acid-concentrations-hamper-bovine-oviductal-epithelial-cell-physiology-in-three-different-in-vitro-culture-systems
#19
L Jordaens, M Arias-Alvarez, I Pintelon, S Thys, S Valckx, Y Dezhkam, P E J Bols, J L M R Leroy
Elevated non-esterified fatty acids (NEFAs) have been recognized as an important link between lipolytic metabolic conditions and impaired fertility in high-yielding dairy cows. However, NEFA effects on the oviductal micro-environment currently remain unknown. We hypothesize that elevated NEFAs may contribute to the complex pathology of subfertility by exerting a negative effect on bovine oviductal epithelial cell (BOEC) physiology. Therefore, the objectives of this study were to elucidate direct NEFA effects on BOEC physiology in three different in vitro cell culture systems...
October 1, 2015: Theriogenology
https://www.readbyqxmd.com/read/26109618/fibronectin-stimulates-human-sperm-capacitation-through-the-cyclic-amp-protein-kinase-a-pathway
#20
E Martínez-León, C Osycka-Salut, J Signorelli, P Pozo, B Pérez, M Kong, P Morales, S Pérez-Martínez, E S Díaz
STUDY QUESTION: Does fibronectin (Fn) stimulate the sperm capacitation process in humans? SUMMARY ANSWER: Fibronectin stimulates human sperm capacitation. WHAT IS KNOWN ALREADY: Capacitation is a process that occurs in the oviduct. It has been suggested that some molecules present in the oviductal fluid and cells as well as proteins present in the cumulus oophorus could be involved in the modulation of sperm function and their acquisition of fertilizing capacity...
September 2015: Human Reproduction
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