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https://www.readbyqxmd.com/read/29691644/mir-101-3p-induces-autophagy-in-endometrial-carcinoma-cells-by-targeting-ezh2
#1
Cuilan Wang, Bo Liu
OBJECTIVE: This study aimed to investigate the effect of miR-101-3p on autophagy in endometrial carcinoma (EC) cells and the connection between miR-101-3p and EZH2. METHODS: The expression levels of miRNAs were analyzed by microarray. The expression level of autophagy related proteins was measured by western blot. The mRNA expression level of beclin-1 was determined by qRT-PCR. Autophagy in EC cells was traced by GFP-LC3 fusion protein and observed by fluorescence microscopy...
April 24, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29691553/-klf10-silencing-inhibited-mechanical-force-induced-osteogenic-differentiation-of-human-periodontal-ligament-cells
#2
Xiao-Qin Yu, Yun-Long Li, Jin-Yu Gao, Hua Liu
PURPOSE: To investigate the effect of Klf10 silence on human periodontal ligament cells (HPDLCs) under mechanical force. METHODS: HPDLCs were isolated and transfected with Klf10 siRNA, and then exposed to centrifugal force for 6 h at 631 r/min. Purmorphamine, an hedgehog signaling pathway agonist, was used for intervention. The activity of alkaline phosphatase (ALP) was detected by ELISA. RT-PCR and Western blot were performed to detect the mRNA and protein expression of Klf10, Runt related transcription factor 2 (Runx2), osteopontin (OPN) and osteocalcin (OCN)...
December 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29691341/epigenetic-regulation-of-tumor-suppressors-by-helicobacter-pylori-enhances-ebv-induced-proliferation-of-gastric-epithelial-cells
#3
Saurabh Pandey, Hem Chandra Jha, Sanket Kumar Shukla, Meghan K Shirley, Erle S Robertson
Helicobacter pylori and Epstein-Barr virus (EBV) are two well-known contributors to cancer and can establish lifelong persistent infection in the host. This leads to chronic inflammation, which also contributes to development of cancer. Association with H. pylori increases the risk of gastric carcinoma, and coexistence with EBV enhances proliferation of infected cells. Further, H. pylori -EBV coinfection causes chronic inflammation in pediatric patients. We have established an H. pylori -EBV coinfection model system using human gastric epithelial cells...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29691336/a-viral-suppressor-modulates-the-plant-immune-response-early-in-infection-by-regulating-microrna-activity
#4
Robert Pertermann, Selvaraj Tamilarasan, Torsten Gursinsky, Giorgio Gambino, Jana Schuck, Claus Weinholdt, Hauke Lilie, Ivo Grosse, Ralph Peter Golbik, Vitantonio Pantaleo, Sven-Erik Behrens
Many viral suppressors (VSRs) counteract antiviral RNA silencing, a central component of the plant's immune response by sequestration of virus-derived antiviral small interfering RNAs (siRNAs). Here, we addressed how VSRs affect the activities of cellular microRNAs (miRNAs) during a viral infection by characterizing the interactions of two unrelated VSRs, the Tombusvirus p19 and the Cucumovirus 2b, with miRNA 162 (miR162), miR168, and miR403. These miRNAs regulate the expression of the important silencing factors Dicer-like protein 1 (DCL1) and Argonaute proteins 1 and 2 (AGO1 and AGO2), respectively...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29691331/gabaergic-local-interneurons-shape-female-fruit-fly-response-to-mating-songs
#5
Daichi Yamada, Hiroshi Ishimoto, Xiaodong Li, Tsunehiko Kohashi, Yuki Ishikawa, Azusa Kamikouchi
Many animals use acoustic signals to attract a potential mating partner. In fruit flies ( Drosophila melanogaster ), the courtship pulse song has a species-specific inter-pulse interval (IPI) that activates mating. Although a series of auditory neurons in the fly brain exhibit different tuning patterns to IPIs, it is unclear how the response of each neuron is tuned. Here we studied the neural circuitry regulating the activity of AMMC-B1 neurons, key secondary auditory neurons in the excitatory neural pathway that relay song information...
April 24, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29691128/ice-parties-among-young-men-who-have-sex-with-men-in-thailand-pleasures-secrecy-and-risks
#6
Thomas E Guadamuz, Pimpawun Boonmongkon
BACKGROUND: Crystal methamphetamine (ice) has become the substance of choice among young men who have sex with men (YMSM) in urban Thailand. Yet, there is scarce data on this phenomenon, partly due to the difficulty in accessing men who will disclose and openly discuss the social contexts, meanings and risks surrounding ice practice. We present an ethnography of ice parties, critically discussing the in-depth social meanings of ice; the sexual socialities and the secrecy surrounding its use; the transactions between older and younger men; and the role of the Internet and mobile technology...
April 21, 2018: International Journal on Drug Policy
https://www.readbyqxmd.com/read/29690669/-lincrna-ror-functions-as-a-cerna-to-regulate-oct4-sox2-and-nanog-expression-by-sponging-mir-145-and-its-effect-on-biologic-characteristics-of-colonic-cancer-stem-cells
#7
Z Y Yan, X C Sun
Objective: To investigate the impact of lincRNA-ROR, a ceRNA by binding miR-145 on the expression of the downstream genes Oct4, Sox2 and Nanog, and related biological characteristics of colon cancer stem cells, and to elucidate the clinical significance of this molecular regulatory network. Methods: Fifty-two cases of colorectal cancer tissue and adjacent tissue were collected at Nanyang City Central Hospital and Nanyang Second Hospital, Henan Province, from 2014 to 2016. Real-time quantitative polymerase chain reaction (qPCR) was used to detect the expression of lincRNA-ROR and miR-145 in colorectal cancer tissue and isolated colon cancer cells...
April 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29690597/liu-jun-zi-tang-a-potential-multi-herbal-complementary-therapy-for-chemotherapy-induced-neurotoxicity
#8
Chun-Tang Chiou, Kaw-Chen Wang, Ying-Chen Yang, Chuen-Lin Huang, Sien-Hung Yang, Yao-Haur Kuo, Nai-Kuei Huang
Liu Jun Zi Tang (LJZT) has been used to treat functional dyspepsia and depression, suggesting its effects on gastrointestinal and neurological functions. LJZT is currently used as a complementary therapy to attenuate cisplatin-induced side effects, such as dyspepsia. However, its effect on chemotherapy-induced neuropathic pain or neurotoxicity has rarely been studied. Thus, we explored potential mechanisms underlying LJZT protection against cisplatin-induced neurotoxicity. We observed that LJZT attenuated cisplatin-induced thermal hyperalgesia in mice and apoptosis in human neuroblastoma SH-SY5Y cells...
April 23, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29690568/mycoviruses-as-triggers-and-targets-of-rna-silencing-in-white-mold-fungus-sclerotinia-sclerotiorum
#9
Pauline Mochama, Prajakta Jadhav, Achal Neupane, Shin-Yi Lee Marzano
This study aimed to demonstrate the existence of antiviral RNA silencing mechanisms in Sclerotinia sclerotiorum by infecting wild-type and RNA-silencing-deficient strains of the fungus with an RNA virus and a DNA virus. Key silencing-related genes were disrupted to dissect the RNA silencing pathway. Specifically, dicer genes ( dcl-1, dcl-2 , and both dcl-1 / dcl-2 ) were displaced by selective marker(s). Disruption mutants were then compared for changes in phenotype, virulence, and susceptibility to virus infections...
April 22, 2018: Viruses
https://www.readbyqxmd.com/read/29689546/eno2-promotes-cell-proliferation-glycolysis-and-glucocorticoid-resistance-in-acute-lymphoblastic-leukemia
#10
Cheng-Cheng Liu, Hua Wang, Wei-da Wang, Liang Wang, Wen-Jian Liu, Jing-Hua Wang, Qi-Rong Geng, Yue Lu
BACKGROUND/AIMS: The metabolic features of cancer cells have long been acknowledged to be altered and to provide new therapeutic opportunities. The expression of glycolytic enzyme enolase 2 (ENO2) was found to be closely associated with the clinical features of acute lymphoblastic leukemia (ALL) patients, but its functions remain unclear in ALL. METHODS: We evaluated the association between ENO2 mRNA expression in bone marrow mononuclear cells (BM-MNCs) and the efficacy of chemotherapy, and further explored the function of ENO2 in ALL...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29689350/hsa_circ_0079530-promotes-cell-proliferation-and-invasion-in-non-small-cell-lung-cancer
#11
Jipeng Li, Jianhua Wang, Zhe Chen, Yin Chen, Mingwei Jin
Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases including cancers. However, little is known about their role in Non-small cell lung cancer (NSCLC). In the present study, we found that hsa_circ_0075930 expression levels were significantly higher in NSCLC cell lines and tissues. The elevated hsa_circ_0075930 expression was correlated with tumor size (P = 0.001) and Lymph node metastasis (P = 0.038). Functional experiments showed knockdown of hsa_circ_0075930 followed by RNA silencing restrained cell proliferation and induced cell cycle arrest of NSCLC cells...
April 21, 2018: Gene
https://www.readbyqxmd.com/read/29689087/identification-of-rna-binding-proteins-in-exosomes-capable-of-interacting-with-different-types-of-rna-rbp-facilitated-transport-of-rnas-into-exosomes
#12
Luisa Statello, Marco Maugeri, Elena Garre, Muhammad Nawaz, Jessica Wahlgren, Alexandros Papadimitriou, Christina Lundqvist, Lennart Lindfors, Anna Collén, Per Sunnerhagen, Marco Ragusa, Michele Purrello, Cinzia Di Pietro, Natalie Tigue, Hadi Valadi
The RNA that is packaged into exosomes is termed as exosomal-shuttle RNA (esRNA); however, the players, which take this subset of RNA (esRNA) into exosomes, remain largely unknown. We hypothesized that RNA binding proteins (RBPs) could serve as key players in this mechanism, by making complexes with RNAs and transporting them into exosomes during the biosynthesis of exosomes. Here, we demonstrate the presence of 30 RBPs in exosomes that were shown to form RNA-RBP complexes with both cellular RNA and exosomal-RNA species...
2018: PloS One
https://www.readbyqxmd.com/read/29688769/il6-and-lif-mrna-expression-in-skeletal-muscle-is-regulated-by-ampk-and-the-transcription-factors-nfyc-zbtb14-and-sp1
#13
Carolina Nylén, Wataru Aoi, Ahmed M Abdelmoez, David G Lassiter, Leonidas S Lundell, Harriet Wallberg-Henriksson, Erik Näslund, Nicolas J Pillon, Anna Krook
AMPK controls glucose- and lipid- metabolism and modulates inflammatory responses to maintain metabolic and inflammatory homeostasis during low cellular energy levels. The AMPK activator 5-aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR) interferes with inflammatory pathways in skeletal muscle, but the mechanisms are undefined. We hypothesized that AMPK activation reduces cytokine mRNA levels by blocking transcription through one or several transcription factors. Three skeletal muscle models were used to study AMPK effects on cytokine mRNA: human skeletal muscle strips obtained from healthy men incubated in vitro, primary human muscle cells and rat L6 cells...
April 24, 2018: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29688643/-fmr1-premutation-carriers-are-they-really-asymptomatic
#14
REVIEW
Shai Elizur, Michal Berkenstadt, Liat Ries-Levavi, Noah Gruber, Orit Pinhas-Hamiel, Sharon Hassin-Baer, Annick Raas-Rothschild, Hila Raanani, Tali Cukierman-Yaffe, Raoul Orvieto, Yoram Cohen, Lidia Gabis
Fragile X Syndrome (FXS), the most common form of inherited mental retardation, is caused by a trinucleotide repeat expansion (CGG) in the 5'-untranslated region of the Fragile X Mental Retardation 1 (FMR1) gene located at Xq27.3. Patients with fragile X -related mental retardation, carry the full mutation CGG-repeat expansions (>200 CGG repeats), which are generally accompanied by hypermethylation of the promoter region, with the consequent transcriptional silencing of the FMR1 gene and absence of the encoded FMR1 protein (FMRP)...
April 2018: Harefuah
https://www.readbyqxmd.com/read/29688601/de-novo-mutations-in-the-set-nuclear-proto-oncogene-encoding-a-component-of-the-inhibitor-of-histone-acetyltransferases-inhat-complex-in-patients-with-non-syndromic-intellectual-disability
#15
Servi Jc Stevens, Vyne van der Schoot, Magalie S Leduc, Tuula Rinne, Seema R Lalani, Marjan M Weiss, Johanna M van Hagen, Augusta Ma Lachmeijer, Sylvia G Stockler-Ipsiroglu, Anna Lehman, Han G Brunner
The role of disturbed chromatin remodelling in the pathogenesis of intellectual disability (ID) is well established and illustrated by de novo mutations found in a plethora of genes encoding for proteins of the epigenetic regulatory machinery. We describe mutations in the "SET nuclear proto-oncogene" (SET), encoding a component of the "inhibitor of acetyltransferases" (INHAT) complex, involved in transcriptional silencing. Using whole exome sequencing, four patients were identified with de novo mutations in the SET gene...
April 24, 2018: Human Mutation
https://www.readbyqxmd.com/read/29688518/analysis-of-the-draft-genome-of-the-red-seaweed-gracilariopsis-chorda-provides-insights-into-genome-size-evolution-in-rhodophyta
#16
JunMo Lee, Eun Chan Yang, Louis Graf, Ji Hyun Yang, Huan Qiu, Udi Zel Zion, Cheong Xin Chan, Timothy G Stephens, Andreas P M Weber, Ga Hun Boo, Sung Min Boo, Kyeong Mi Kim, Younhee Shin, Myunghee Jung, Seung Jae Lee, Hyung-Soon Yim, Jung-Hyun Lee, Debashish Bhattacharya, Hwan Su Yoon
Red algae (Rhodophyta) underwent two phases of large-scale genome reduction during their early evolution. The red seaweeds did not attain genome sizes or gene inventories typical of other multicellular eukaryotes. We generated a high quality 92.1 Mbp draft genome assembly from the red seaweed Gracilariopsis chorda, including methylation and small (s)RNA data. We analyzed these and other Archaeplastida genomes to address three questions: 1) what is the role of repeats and transposable elements (TEs) in explaining Rhodophyta genome size variation, 2) what is the history of genome duplication and gene family expansion/reduction in these taxa, and 3) is there evidence for TE suppression in red algae? We find the number of predicted genes in red algae is relatively small (4,803-13,125 genes), particularly when compared to land plants, with no evidence of polyploidization...
April 23, 2018: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29688285/neural-coding-of-cell-assemblies-via-spike-timing-self-information
#17
Meng Li, Kun Xie, Hui Kuang, Jun Liu, Deheng Wang, Grace E Fox, Zhifeng Shi, Liang Chen, Fang Zhao, Ying Mao, Joe Z Tsien
Cracking brain's neural code is of general interest. In contrast to the traditional view that enormous spike variability in resting states and stimulus-triggered responses reflects noise, here, we examine the "Neural Self-Information Theory" that the interspike-interval (ISI), or the silence-duration between 2 adjoining spikes, carries self-information that is inversely proportional to its variability-probability. Specifically, higher-probability ISIs convey minimal information because they reflect the ground state, whereas lower-probability ISIs carry more information, in the form of "positive" or "negative surprisals," signifying the excitatory or inhibitory shifts from the ground state, respectively...
April 21, 2018: Cerebral Cortex
https://www.readbyqxmd.com/read/29687844/peroxiredoxin1-promotes-cell-proliferation-migration-and-invasion-of-colorectal-cancer-via-p38mapk-signaling
#18
N Cong, W Huang, J-P Yuan, G-Z Li, G-S Zhai, B-S Li
OBJECTIVE: Peroxiredoxin1 (PRDX1), a class of thiol peroxidases, is a multifunctional protein. We aimed at analyzing the effect of PRDX1 on proliferation, apoptosis, migration and invasion of colorectal cancer and to investigate the potential mechanism. MATERIALS AND METHODS: Western blot and PCR were used to validate the silencing efficiency in SW480 cell by transfection of PRDX1-siRNA. The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) test. Flow cytometry Annexin V/PI double staining was used to analyze cell apoptosis...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29687822/probing-the-nanoparticle-ago2-interaction-for-enhanced-gene-knockdown
#19
Sonal Deshpande, Neetu Singh
RNAi is emerging as a promising technology for treatment of various diseases due to its ability to silence specific target genes. To date, a number of nanoparticle based formulations have been reported for the delivery of small interfering RNA (siRNA), with continuous modifications in the nanoparticle design for enhancing their efficiency. While majority of the design aspects are focused on avoiding or overcoming endosomal entrapment, limited studies are available that address the role of interaction of nanoparticles with the RNA induced silencing complex (RISC) machinery, which is a crucial aspect deciding the outcome...
April 24, 2018: Soft Matter
https://www.readbyqxmd.com/read/29687307/up-regulation-of-cdc37-contributes-to-schwann-cell-proliferation-and-migration-after-sciatic-nerve-crush
#20
Yuxi Liu, Shuyao Wang, Dazhi Ding, Zhaohui Yu, Weiwei Sun, Youhua Wang
Cell division cycle protein 37 (Cdc37), a molecular chaperone takes part in a series of cellular processes including cell signal transduction, cell cycle progression, cell proliferation, cell motility, oncogenesis and malignant progression. It can not only recruit immature protein kinases to HSP90 but also work alone. Cdc37 was reported to be associated with neurogenesis, neurite outgrowth, axon guidance and myelination. However, the roles of Cdc37 on Schwann cells (SC) after peripheral nerve injury (PNI) remain unknown...
April 23, 2018: Neurochemical Research
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