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Micro RNA

Shamila D Alipoor, Esmaeil Mortaz, Payam Tabarsi, Majid Marjani, Mohammad Varahram, Gert Folkerts, Johan Garssen, Ian M Adcock
Tuberculosis (TB) remains a major threat to human health. Understanding the strategies mycobacteria take to overcome immune defense is important in order to control the infection. Micro (mi)RNAs are master regulators of most pathways in the human body.  Infection with mycobacterium impacts upon the host metabolic pathways as they are subverted to obtain the nutrition for intracellular TB survival. In this study, we aimed to investigate the effect of Bacillus Calmette-Guérin (BCG) infection on the expression of three miRNAs (miR-1224, -484 and -425), which are important in infection and in the regulation of metabolic pathways...
June 2018: Iranian Journal of Allergy, Asthma, and Immunology
Muqing Gu, Lijuan Wang, Chun Yang, Xue Li, Chanwei Jia, Stephane Croteau, Xiangyan Ruan, Pierre Hardy
BACKGROUND: Recent investigations have indicated that hormone therapy may increase the risk of breast cancer (BC), and the addition of synthetic progestins may play a critical role in this. Several studies have pointed out the important role of progesterone receptor membrane component 1 (PGRMC1) in the development of BC, especially with hormone therapy using progestins. Although the deregulation of microRNA-181a (miR-181a) is often associated with human BC, the effect of miR-181a on PGRMC1 expression during hormone therapy has not been investigated...
August 2018: Maturitas
Víctor Manuel Vargas-Aguilar, Karina Arroyo-Alvarez
The genes assay provides a clearer picture of individual tumor biology, hormonal- receptor and proliferation. Triple negative breast cancer have high proliferation index and no prognostic with this test. The first generation of gene assay (OncotypeDX, MammaPrint) predice recurrence at 5 years. The next generation (Prosigna, Endopredict) have better prognosis value for recurrence and predictive of early relapse. There are no useful genetic tests for tumors hormonal- negative with pronostic and predictive value...
March 2018: Revista Médica del Instituto Mexicano del Seguro Social
Shuai Lv, Meng Guan
Previous literatures have reported the role of human micro RNA-1284 (hsa-miR-1284, in short miR-1284) in diverse cancers. However, its biological function in osteosarcoma pathogenesis remains unknown. In this study, we investigated the potential role of miR-1284 in osteosarcoma. Expression of miR-1284 and HMGB1 were examined in 80 tissues obtained from 40 patients. MiR-1284 level was measured in 5 osteosarcoma cell lines. Relative luciferase activity and HMGB1 expression were examined in MG-63 and U2OS cells transfected with wild type or mutant 3'-UTR of HMGB1 in the presence of miR-1284 mimics or miR-NC...
June 13, 2018: Bioscience Reports
Qin Li, Baolin Yao, Alexander Endler, Li Chen, Futoshi Shibasaki, Haidong Cheng
We investigated whether stable eukaryotic translation initiation factor 3e/inter 6 (eIF-3e/Int6) RNA-silencing (siRNA-Int6) can ameliorate pre-eclampsia (PE) by promoting angiogenesis in an N-nitro-L-arginine methyl ester (L-NAME)-induced rat pre-eclampsia (PE) model. Twenty-four pregnant female Sprague-Dawley rats were allocated into 4 groups, including controls (Con) without any treatment, and 18 from gestational day (GD) 7 to GD17 L-NAME-treated rats, which were divided into stable siRNA-Int6 transfected (siRNA-Int6), negative vector control siRNA (NC-siRNA) and PE control (PE-Con) groups...
June 12, 2018: Scientific Reports
Jose Coelho-Lima, Ashfaq Mohammed, Suzanne Cormack, Samuel Jones, Rajiv Das, Mohaned Egred, Pedram Panahi, Simi Ali, Ioakim Spyridopoulos
BACKGROUND:  Cardiac-enriched micro ribonucleic acids (miRNAs) are released into the circulation following ST-elevation myocardial infarction (STEMI). Lack of standardized approaches for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) data normalization and presence of RT-qPCR inhibitors (e.g. heparin) in patient blood samples have prevented reproducible miRNA quantification in this cohort and subsequent translation of these biomarkers to clinical practice...
June 11, 2018: Thrombosis and Haemostasis
C Shen, Q Z Wang, Z Y Shen, H Y Yuan, W J Yu, X D Chen, H Xu
BACKGROUND: Acne vulgaris is a common chronic skin disease. Inflammation is an important pathogenetic mechanism of acne, and NLRP3 polymorphisms have been reported to be involved in the mediation and occurrence of the inflammation. However, only a few studies on NLRP3 and acne have been reported, and the mechanism remains unclear. AIM: To investigate two SNPs in the NLRP3 gene in patients with acne vulgaris (AV) and healthy controls (HCs) in a Chinese population...
June 11, 2018: Clinical and Experimental Dermatology
Aidan Kenny, Eva M Jimenez-Mateos, Miguel Calero, Miguel Medina, Tobias Engel
Alzheimer's disease is the most common form of dementia and is characterized by a progressive loss of cognitive functions. As the result of predicted demographic changes over the next decades, Alzheimer's disease is expected to be one of the most pressing medical and social challenges facing our generation. Current treatment strategies remain symptomatic. However, new approaches have shown promise in clinical trials, particularly in patients with only mild or moderate symptoms. Early detection of Alzheimer's disease is therefore of critical importance...
2018: Methods in Molecular Biology
Peter D Fransquet, Joanne Ryan
As the prevalence of Alzheimer's disease (AD) increases, the search for a definitive, easy to access diagnostic biomarker has become increasingly important. Micro RNA (miRNA), involved in the epigenetic regulation of protein synthesis, is a biological mark which varies in association with a number of disease states, possibly including AD. Here we comprehensively review methods and findings from 26 studies comparing the measurement of miRNA in blood between AD cases and controls. Thirteen of these studies used receiver operator characteristic (ROC) analysis to determine the diagnostic accuracy of identified miRNA to predict AD, and three studies did this with a machine learning approach...
June 6, 2018: Clinical Biochemistry
Virginia H Huxley, Scott S Kemp, Christine Schramm, Steve Sieveking, Susan Bingaman, Yang Yu, Isabella Zaniletti, Kevin Stockard, Jianjie Wang
KEY POINTS: Endothelial dysfunction is an early hallmark of multiple disease states that also display sex differences with respect to age of onset, frequency, and severity. Results of in vivo studies of basal and stimulated microvascular barrier function revealed sex differences difficult to ascribe to specific cells or environmental factors. The present study evaluated endothelial cells (EC) isolated from macro- and/or microvessels of reproductively mature rats under the controlled conditions of low-passage culture to test the assumption that EC phenotype would be sex-independent...
June 9, 2018: Journal of Physiology
Steven D Hicks, Paige Jacob, Frank A Middleton, Omar Perez, Zofia Gagnon
Micro-ribonucleic acids (miRNAs) mediate adaptive responses to exercise and may serve as biomarkers of exercise intensity/capacity. Expression of miRNAs is altered in skeletal muscle, plasma, and saliva following exertion. Females display unique physiologic responses to endurance exercise, and miRNAs respond to pathologic states in sex-specific patterns. However sex-specific miRNA responses to exercise remain unexplored. This study utilized high-throughput RNA sequencing to measure changes in salivary RNA expression among 25 collegiate runners following a single long-distance run...
June 8, 2018: Physiological Genomics
F Zhang, M Suzuki, I S Kim, R Kobayashi, N Hamada, F Sato, U K Bhawal
BACKGROUND AND OBJECTIVES: Disruption of transcriptional regulation is a confounding factor associated with a wide range of human inflammatory diseases. To investigate mechanistic links between transcription factor DEC1 and pathways underlying inflammation, wild-type and DEC1 knockout (KO) C57BL/6 mice were treated with Porphyromonas gingivalis (or carboxymethyl cellulose as a control) to induce periodontal inflammation. It provoked an inflammatory response within the oral environment, which showed robust variation in alveolar bone resorption and expression of inflammatory cytokines...
June 7, 2018: Journal of Periodontal Research
Ewa St Ecedil Pień, Marina C Costa, Szczepan Kurc, Anna Drożdż, Nuno Cortez-Dias, Francisco J Enguita
Pervasive transcription of the human genome is responsible for the production of a myriad of non-coding RNA molecules (ncRNAs) some of them with regulatory functions. The pivotal role of ncRNAs in cardiovascular biology has been unveiled in the last decade, starting from the characterization of the involvement of micro-RNAs in cardiovascular development and function, and followed by the use of circulating ncRNAs as biomarkers of cardiovascular diseases. The human non-coding secretome is composed by several RNA species that circulate in body fluids and could be used as biomarkers for diagnosis and outcome prediction...
June 7, 2018: Acta Pharmacologica Sinica
Kathryn S Nevel, Jessica A Wilcox, Lindsay J Robell, Yoshie Umemura
PURPOSE OF REVIEW: Liquid biopsy is a sampling of tumor cells or tumor nucleotides from biofluids. This review explores the roles of liquid biopsy for evaluation and management of patients with primary and metastatic CNS malignancies. RECENT FINDINGS: Circulating tumor cell (CTC) detection has emerged as a relatively sensitive and specific tool for diagnosing leptomeningeal metastases. Circulating tumor DNA (ctDNA) detection can effectively demonstrate genetic markup of CNS tumors in the cerebrospinal fluid, though its role in managing CNS malignancies is less well-defined...
June 6, 2018: Current Oncology Reports
T N Uma Maheswari, Archana Venugopal, Nivedhita Malli Sureshbabu, Prathiba Ramani
Oral potentially malignant disorders (OPMD) are oral mucosal disorders which have a high potential to turn into malignancy. A recent report suggests that 16%-62% of epithelial dysplasia cases of OPMD undergo malignant transformation, showing the need for early detection of malignancy in these disorders. Micro RNA (miRNA) plays an important role in cellular growth, differentiation, apoptosis, and immune response, and hence, deregulation of miRNA is considered a signature of oral carcinogenesis. A search was done using MeSH terms in the PubMed, ScienceDirect databases, hand search, and finally, six studies were included in this systematic review...
April 2018: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
Jeremy A Dubin, Daniel R Greenberg, Kag C Iglinski-Benjamin, Geoffrey D Abrams
The purpose of the review was to synthesize the current literature regarding the effect of miRNA on biological processes known to be involved in tendon and tenocyte development and homeostasis. Using multiple databases, a systematic review was performed with a customized search term crafted to identify any study examining micro-RNA in relation to tendon and/or tenocytes. Results were classified based on the following categories: gene expression, tenocyte development and differentiation, tendon tissue repair, and tenocyte senescence...
June 6, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Han Yu, Houwen Matthew Pan, Fnu Evalin, Dieter Wilhelm Trau, Volker Patzel
The breakthrough of genetic therapy is set back by the lack of suitable genetic vector systems. We present the development of permeability-tunable, capsule-like, polymeric, micron-sized, core-shell particles for delivery of recombinant nucleic acids into target cells. These particles were demonstrated to effectively release rod-shaped small hairpin RNA and to selectively retain the RNA-encoding DNA template which was designed to form a bulky tripartite structure. Thus, they can serve as delivery vectors preloaded with cargo RNA or alternatively as RNA producing micro-bioreactors...
June 5, 2018: ACS Applied Materials & Interfaces
Raymond Farah, Hayat Haraty, Ziad Salame, Youssef Fares, David M Ojcius, Najwane Said Sadier
Current research efforts on neurological diseases are focused on identifying novel disease biomarkers to aid in diagnosis, provide accurate prognostic information and monitor disease progression. With advances in detection and quantification methods in genomics, proteomics and metabolomics, saliva has emerged as a good source of samples for detection of disease biomarkers. Obtaining a sample of saliva offers multiple advantages over the currently tested biological fluids as it is a non-invasive, painless and simple procedure that does not require expert training or harbour undesirable side effects for the patients...
April 2018: Biomedical Journal
Meina Neumann-Schaal, Nicole G Metzendorf, Daniel Troitzsch, Aaron Mischa Nuss, Julia Danielle Hofmann, Michael Beckstette, Petra Dersch, Andreas Otto, Susanne Sievers
Clostridioides difficile is the major pathogen causing diarrhea following antibiotic treatment. It is considered to be a strictly anaerobic bacterium, however, previous studies have shown a certain and strain-dependent oxygen tolerance. In this study, the model strain C. difficile 630Δerm was shifted to micro-aerobiosis and was found to stay growing to the same extent as anaerobically growing cells with only few changes in the metabolite pattern. However, an extensive change in gene expression was determined by RNA-Seq...
May 31, 2018: Anaerobe
Xiaonan Ma, Ying Zhang, Fang Qiao, Mengyun Wang, Zhenzi Zuo, Yong Zhang, Yongsheng Wang
RT-qPCR is a widely used method to detect miRNA expression. Compared with mRNA, miRNA has a shorter length and lower abundance which hinders the acquisition of reliable results. Thus, miRNA detection requires a method with high sensitivity and accuracy. Collecting large amounts of material is particularly difficult for oocytes and pre-implantation embryos of domestic animals. Establishing a set of miRNA detection methods that are suitable to detect trace amounts of such materials is urgently needed. In this study, the total RNA in 50 germinal vesicle (GV) oocytes was isolated through direct lysis and by using mirVana miRNA Isolation Kit and miRNeasy Micro Kit...
May 19, 2018: Animal Reproduction Science
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