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https://www.readbyqxmd.com/read/29687863/mir-1908-improves-cardiac-fibrosis-after-myocardial-infarction-by-targeting-tgf-%C3%AE-1
#1
Y Chen, T Li, Q Gao, L-Y Wang, L-Q Cui
OBJECTIVE: To investigate the role and mechanism of micro ribonucleic acid (miR)-1908 in myocardial fibrosis after myocardial infarction. MATERIALS AND METHODS: In in-vivo experiments, the rat model of myocardial infarction was established, and miR-1908 was up-regulated by lentivirus with miR-1908 overexpression. Cardiac function of rats was detected by echocardiography. Transforming growth factor-β1 (TGF-β1) and Smad2/3 expressions in infarction border zone were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29686252/micro-rnas-from-dna-viruses-are-found-widely-in-plasma-in-a-large-observational-human-population
#2
Milka Koupenova, Eric Mick, Heather A Corkrey, Tianxiao Huan, Lauren Clancy, Ravi Shah, Emelia J Benjamin, Daniel Levy, Evelyn A Kurt-Jones, Kahraman Tanriverdi, Jane E Freedman
Viral infections associate with disease risk and select families of viruses encode miRNAs that control an efficient viral cycle. The association of viral miRNA expression with disease in a large human population has not been previously explored. We sequenced plasma RNA from 40 participants of the Framingham Heart Study (FHS, Offspring Cohort, Visit 8) and identified 3 viral miRNAs from 3 different human Herpesviridae. These miRNAs were mostly related to viral latency and have not been previously detected in human plasma...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29685897/characterization-of-human-salivary-extracellular-rna-by-next-generation-sequencing
#3
Feng Li, Karolina Elżbieta Kaczor-Urbanowicz, Jie Sun, Blanca Majem, Hsien-Chun Lo, Yong Kim, Kikuye Koyano, Shannon Liu Rao, So Young Kang, Su Mi Kim, Kyoung-Mee Kim, Sung Kim, David Chia, David Elashoff, Tristan R Grogan, Xinshu Xiao, David T W Wong
BACKGROUND: It was recently discovered that abundant and stable extracellular RNA (exRNA) species exist in bodily fluids. Saliva is an emerging biofluid for biomarker development for noninvasive detection and screening of local and systemic diseases. Use of RNA-Sequencing (RNA-Seq) to profile exRNA is rapidly growing; however, no single preparation and analysis protocol can be used for all biofluids. Specifically, RNA-Seq of saliva is particularly challenging owing to high abundance of bacterial contents and low abundance of salivary exRNA...
April 23, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29684582/platelet-microparticle-delivered-microrna-let-7a-promotes-the-angiogenic-switch
#4
Chinedu Anene, Anne M Graham, James Boyne, Wayne Roberts
Platelet microparticle (PMP)-induced angiogenesis plays a key role in tumour metastasis and has been proposed to contribute towards cardiovascular disease by enhancing atherosclerotic plaque vulnerability. However, the mechanisms underlying PMP induced angiogenesis are ill defined. Recent reports demonstrate that PMPs deliver micro-RNAs (miRNAs) to recipient cells, controlling gene expression. We therefore evaluated whether miRNA transfer was a key regulator of PMP-induced angiogenesis. Co-culturing PMPs with human umbilical vein endothelial cells (HUVEC) on extracellular matrix gel induced robust capillary like structure formation...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29679926/peripheral-artery-disease-a-micro-rna-related-condition
#5
REVIEW
Georgia Vogiatzi, Evangelos Oikonomou, Spyridon Deftereos, Gerasimos Siasos, Dimitris Tousoulis
Peripheral artery disease (PAD) is a vascular problem of diffuse atherosclerosis and is a major cause of cardiovascular morbidity and mortality. Many studies have tried to gain insight into the molecular mechanisms involved in PAD. Lately, highly stable circulating small noncoding RNAs, microRNAs (miRNAs), seem to have disease-specific expression. Thus, miRNAs are emerging as new measurable diagnostic biomarkers and a start point for individualized treatment. To date, the association of miRNA regulation in angiogenesis and maintenance of vascular integrity in PAD has attracted little interest...
April 18, 2018: Current Opinion in Pharmacology
https://www.readbyqxmd.com/read/29678959/extracellular-vesicles-and-their-content-in-bioactive-lipid-mediators-more-than-a-sack-of-microrna
#6
Eric Boilard
Extracellular vesicles (EVs), such as exosomes and microvesicles, are small membrane-bound vesicles released by cells under various conditions. In a multitude of physiological and pathological conditions, EVs contribute to intercellular communication by facilitating exchange of material between cells. Rapidly growing interest is aimed at better understanding EV function and their use as biomarkers. The vast EV cargo includes cytokines, growth factors, organelles, nucleic acids (messenger and micro RNA), and transcription factors...
April 20, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29673856/2-3-7-8-tetrachlorodibenzo-p-dioxin-dose-dependently-increases-bone-mass-and-decreases-marrow-adiposity-in-juvenile-mice
#7
Kelly A Fader, Rance Nault, Sandi Raehtz, Laura R McCabe, Timothy R Zacharewski
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and other aryl hydrocarbon receptor (AhR) agonists have been shown to regulate bone development and remodeling in a species-, ligand-, and age-specific manner, however the underlying mechanisms remain poorly understood. In this study, we characterized the effect of 0.01-30 μg/kg TCDD on the femoral morphology of male and female juvenile mice orally gavaged every 4 days for 28 days and used RNA-Seq to investigate gene expression changes associated with the resultant phenotype...
April 16, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29669595/imp1-regulates-uca1-mediated-cell-invasion-through-facilitating-uca1-decay-and-decreasing-the-sponge-effect-of-uca1-for-mir-122-5p
#8
Yanchun Zhou, Xiuhua Meng, Shaoying Chen, Wei Li, Delin Li, Robert Singer, Wei Gu
BACKGROUND: Long noncoding RNAs (LncRNAs) represent a class of widespread and diverse endogenous RNAs that can posttranscriptionally regulate gene expression through the interaction with RNA-binding proteins and micro RNAs (miRNAs). Here, we report that in breast carcinoma cells, the insulin-like growth factor 2 messenger RNA binding protein (IMP1) binds to lncRNA urethral carcinoma-associated 1 (UCA1) and suppresses the UCA1-induced invasive phenotype. METHODS: RT-qPCR and RNA sequence assays were used to investigate the expression of UCA1 and miRNAs in breast cancer cells in response to IMP1 expression...
April 18, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29669525/salivary-extracellular-vesicle-associated-mirnas-as-potential-biomarkers-in-oral-squamous-cell-carcinoma
#9
Chiara Gai, Francesco Camussi, Roberto Broccoletti, Alessio Gambino, Marco Cabras, Luca Molinaro, Stefano Carossa, Giovanni Camussi, Paolo G Arduino
BACKGROUND: Several studies in the past have investigated the expression of micro RNAs (miRNAs) in saliva as potential biomarkers. Since miRNAs associated with extracellular vesicles (EVs) are known to be protected from enzymatic degradation, we evaluated whether salivary EVs from patients with oral squamous cell carcinoma (OSCC) were enriched with specific subsets of miRNAs. METHODS: OSCC patients and controls were matched with regards to age, gender and risk factors...
April 18, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29660943/circulating-cell-free-micro-rna-profiles-reflect-discordant-development-of-dementia-in-monozygotic-twins
#10
Jonas Mengel-From, Mette E Rønne, Anting L Carlsen, Kristin Skogstrand, Lisbeth A Larsen, Qihua Tan, Lene Christiansen, Kaare Christensen, Niels H H Heegaard
We aim to examine if circulating micro-RNA and cytokine levels associate with dementia diagnosis and cognitive scores. To test our hypothesis, we use plasma donated from 48 monozygotic twin pairs in 1997 and 46 micro-RNAs and 10 cytokines were quantified using microfluidic RT-qPCR and multiplex solid-phase immunoassays, respectively. Micro-RNA and cytokine profiling were examined for associations with dementia diagnoses in a longitudinal registry study or with cognitive scores at baseline. Thirty-six micro-RNAs and all cytokines were detected consistently...
April 11, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29659812/the-genome-sequence-and-transcriptome-of-potentilla-micrantha-and-their-comparison-to-fragaria-vesca-the-woodland-strawberry
#11
Matteo Buti, Marco Moretto, Elena Barghini, Flavia Mascagni, Lucia Natali, Matteo Brilli, Alexandre Lomsadze, Paolo Sonego, Lara Giongo, Michael Alonge, Riccardo Velasco, Claudio Varotto, Nada Šurbanovski, Mark Borodovsky, Judson A Ward, Kristof Engelen, Andrea Cavallini, Alessandro Cestaro, Daniel James Sargent
Background: The genus Potentilla is closely related to that of Fragaria, the economically important strawberry genus. Potentilla micrantha is a species that does not develop berries but shares numerous morphological and ecological characteristics with Fragaria vesca. These similarities make P. micrantha an attractive choice for comparative genomics studies with F. vesca. Findings: In this study, the P. micrantha genome was sequenced and annotated, and RNA-Seq data from the different developmental stages of flowering and fruiting were used to develop a set of gene predictions...
April 1, 2018: GigaScience
https://www.readbyqxmd.com/read/29658923/micro-dissection-of-enamel-organ-from-mandibular-incisor-of-rats-exposed-to-environmental-toxicants
#12
Sophia Houari, Sylvie Babajko, Sophia Loiodice, Ariane Berdal, Katia Jedeon
Enamel defects resulting from environmental conditions and ways of life are public health concerns because of their high prevalence. These defects result from altered activity of cells responsible for enamel synthesis named ameloblasts, which present in enamel organ. During amelogenesis, ameloblasts follow a specific and precise sequence of events of proliferation, differentiation, and death. A rat continually growing incisors is a suitable experimental model to study ameloblast activity and differentiation stages in physiological and pathological conditions...
March 29, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29658582/sirt1-nrf2-signalling-pathway-prevents-cognitive-impairment-in-diabetic-rats-through-anti%C3%A2-oxidative-stress-induced-by-mirna%C3%A2-23b%C3%A2-3p-expression
#13
Jinhua Han, Xiaoning Liu, Yingzhi Li, Jun Zhang, Haichi Yu
In the present study the exact roles and mechanisms underlying the effect of miRNA‑23b‑3p on the cognitive impairment of diabetic rats were investigated. The in vivo model of diabetes was established in Wistar rats via a single injection of streptozotocin (STZ). Cognitive function was evaluated using a Morris water maze test. Oxidative stress was measured using ELISA kits, and the protein expression levels of B‑cell lymphoma 2‑associated X protein, silent information regulator 1 (SIRT1), nuclear factor erythroid 2‑related factor 2 (Nrf2) and GAPDH were measured by western blot analysis...
April 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29621968/effects-of-micro-rnas-and-their-targets-in-periodontal-diseases
#14
Samane Bourbour, Maryam Beheshti, Hossein Kazemian, Abbas Bahador
: RNA interference is a strong mechanism of gene silencing which underlies many fields of eukaryotic biology (1). MicroRNAs (miRNAs) are small noncoding RNAs and a gene regulation expression system showing a tissue-specific expression pattern, and inhibits protein synthesis (2). For the first time, miRNA has been seen in Caenorhabditis elegans (3). They play an important role in signaling pathways of various diseases especially periodontal disease and cancers (4). One of the most important diseases in association with gingival infectious is periodontal diseases, which if untreated leads to tooth loss, hence in present paper focus on the effects of MicroRNAs and their targets in disease which was mentioned above...
April 5, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29620241/microarray-expression-profile-analysis-of-circular-rnas-in-pancreatic-cancer
#15
Shixiang Guo, Xuejun Xu, Yongsheng Ouyang, Yunchao Wang, Jiali Yang, Liangyu Yin, Jiayun Ge, Huaizhi Wang
Pancreatic cancer remains one of the most malignant tumors with a poor prognosis. Despite advances in diagnosis and treatment, no reliable biomarkers are available for clinical practice. Circular RNAs (circRNAs) are a novel class of endogenous non‑coding RNA, which are abundant, stable and conserved, and serve crucial roles in disease, particularly in cancer. The purpose of the present study was to investigate the expression profile of circRNAs in 20 pancreatic cancer tissues and corresponding paracancerous tissues using arraystar human circRNA array analysis, high‑throughput circRNA microarray, bioinformatic analysis and reverse transcription‑quantitative polymerase chain reaction...
March 29, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29609653/the-active-microbial-community-more-accurately-reflects-the-anaerobic-digestion-process-16s-rrna-gene-sequencing-as-a-predictive-tool
#16
Jo De Vrieze, Ameet J Pinto, William T Sloan, Umer Zeeshan Ijaz
BACKGROUND: Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion. These methods successfully characterize amplicons but do not distinguish micro-organisms that are actually responsible for the process. In this research, the archaeal and bacterial community of 48 full-scale anaerobic digestion plants were evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing...
April 2, 2018: Microbiome
https://www.readbyqxmd.com/read/29605406/micro-rna-profile-and-proteins-in-peritoneal-fluid-from-women-with-endometriosis-their-relationship-with-sterility
#17
Josep Marí-Alexandre, Moisés Barceló-Molina, Elisa Belmonte-López, Javier García-Oms, Amparo Estellés, Aitana Braza-Boïls, Juan Gilabert-Estellés
OBJECTIVE: To define the microRNA (miRNA) profile and its relationship with cytokines content in peritoneal fluid (PF) from endometriosis patients. DESIGN: Case-control study. SETTING: University hospital, research institute. PATIENT(S): One hundred twenty-six women with endometriosis (EPF) and 45 control women (CPF). MAIN OUTCOMES MEASURE(S): MiRNA arrays were prepared from six EPF and six CPF...
March 28, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29600608/-circrna-as-a-new-field-in-human-disease-research
#18
REVIEW
Jia-Liang Gao, Guang Chen, Hao-Qiang He, Jie Wang
For the characteristics of high stability, high conservation between species, and tissue specificity, circular RNA(circRNA) has been expected to become a new molecular biomarker for the clinical diagnosis, treatment and prognosis, and the potential target for targeted therapy. Study on the traditional Chinese medicine(TCM) syndrome includes not only the macro indexes collected by using four traditional methods of diagnosis, but also contains some micro information that can not be achieved by using the four traditional diagnosis methods...
February 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29596348/new-gene-markers-for-metabolic-processes-and-homeostasis-in-porcine-buccal-pouch-mucosa-during-cells-long-term-cultivation-a-primary-culture-approach
#19
Marta Dyszkiewicz-Konwińska, Mariusz J Nawrocki, Yan Huang, Artur Bryja, Piotr Celichowski, Maurycy Jankowski, Katarzyna Błochowiak, Katarzyna Mehr, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
The oral mucosal tissue is a compound structure composed of morphologically and physiologically different cell types. The morphological modification involves genetically determined lifespan, which may be recognized as the balance between cell survival and apoptosis. Although the biochemical processes and pathways in oral mucosa, with special regards to drug transport, delivery, and metabolism, are well known, the cellular physiological homeostasis in this tissue requires further investigation. The porcine buccal pouch mucosal cells (BPMCs) collected from 20 pubertal crossbred Landrace gilts, were used in this study...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29589310/rt-qpcr-for-fecal-mature-microrna-quantification-and-validation
#20
Farid E Ahmed, Nancy C Ahmed, Mostafa M Gouda, Paul W Vos, Chris Bonnerup
By routinely and systematically being able to perform quantitative stem-loop reverse transcriptase (RT) followed by TaqMan® minor-groove binding (MGB) probe, real-time quantitative PCR analysis on exfoliated enriched colonocytes in stool, using human (Homo sapiens, hsa) micro(mi)RNAs to monitor changes of their expression at various stages of colorectal (CRC) progression, this method allows for the reliable and quantitative diagnostic screening of colon cancer (CC). Although the expression of some miRNA genes tested in tissue shows less variability in normal or cancerous patients than in stool, the noninvasive stool by itself is well suited for CC screening...
2018: Methods in Molecular Biology
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