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https://www.readbyqxmd.com/read/29453955/cross-reactivity-of-anti-hmgb1-antibodies-for-hmgb2
#1
Jessica E Davies, Bonita H R Apta, Matthew T Harper
HMGB1 and HMGB2 are DNA-interacting proteins but can also have extracellular actions during inflammation. Despite their relatively high homology, they may have distinct roles, making it essential to be able to differentiate between the two. Here we examine the specificity of five commercially-available anti-HMGB1 antibodies. By Western blotting of recombinant proteins and HMGB1-/- mouse embryonic fibroblasts, we identified only one HMGB1 antibody that, under our experimental conditions, did not also detect HMGB2...
February 14, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29453167/electrochemical-detection-of-methylated-dna-on-a-microfluidic-chip-with-nanoelectrokinetic-pre-concentration
#2
Sung A Hong, Yong-June Kim, Sung Jae Kim, Sung Yang
DNA methylation is considered to be a promising marker for the early diagnosis and prognosis of cancer. However, direct detection of the methylated DNAs in clinically relevant samples is still challenging because of its extremely low concentration (~fM). Here, an integrated microfluidic chip is reported, which is capable of pre-concentrating the methylated DNAs using ion concentration polarization (ICP) and electrochemically detecting the pre-concentrated DNAs on a single chip. The proposed chip is the first demonstration of an electrochemical detection of both level and concentration of the methylated DNAs by integrating a DNA pre-concentration unit without gene amplification...
February 1, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29452855/constituents-of-neutrophil-extracellular-traps-induce-in-vitro-collagen-formation-in-mare-endometrium
#3
Maria Rosa Rebordão, Ana Amaral, Karolina Lukasik, Anna Szóstek-Mioduchowska, Pedro Pinto-Bravo, António Galvão, Dariusz J Skarzynski, Graça Ferreira-Dias
Neutrophil extracellular traps (NETs) are DNA complexes carrying nuclear and cytoplasmic proteins, such as elastase (ELA), cathepsin-G (CAT) and myeloperoxidase (MPO). Mare endometrosis is a chronic degenerative process characterized by excessive collagen in endometrium. While NETs fight bacteria that cause endometritis, they may trigger endometrial fibrogenesis. The aim was to evaluate the in vitro effect of some NETs components on mare endometrial fibrogenesis and determine its relationship with histopathology or estrous cycle...
February 7, 2018: Theriogenology
https://www.readbyqxmd.com/read/29452097/stimulation-of-trpv1-channels-activates-the-ap-1-transcription-factor
#4
Tobias M Backes, Oliver G Rössler, Xin Hui, Carsten Grötzinger, Peter Lipp, Gerald Thiel
Transient receptor potential vanilloid 1 (TRPV1) channels were originally described as the receptors of capsaicin, the main constituent of hot chili pepper. The biological functions of TRPV1 channels include pain sensation and inflammatory thermal hyperalgesia. Here, we show that stimulation of HEK293 cells expressing TRPV1 channels (H2C1 cells) with capsaicin or the TRPV1 ligand resiniferatoxin activated transcription mediated by the transcription factor AP-1. No cell death was occurring under these experimental conditions...
February 13, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29451920/an-upstream-sequence-modulates-phenazine-production-at-the-level-of-transcription-and-translation-in-the-biological-control-strain-pseudomonas-chlororaphis-30-84
#5
Jun Myoung Yu, Dongping Wang, Tessa R Ries, Leland S Pierson, Elizabeth A Pierson
Phenazines are bacterial secondary metabolites and play important roles in the antagonistic activity of the biological control strain P. chlororaphis 30-84 against take-all disease of wheat. The expression of the P. chlororaphis 30-84 phenazine biosynthetic operon (phzXYFABCD) is dependent on the PhzR/PhzI quorum sensing system located immediately upstream of the biosynthetic operon as well as other regulatory systems including Gac/Rsm. Bioinformatic analysis of the sequence between the divergently oriented phzR and phzX promoters identified features within the 5'-untranslated region (5'-UTR) of phzX that are conserved only among 2OHPCA producing Pseudomonas...
2018: PloS One
https://www.readbyqxmd.com/read/29449612/ixr1-regulates-ribosomal-gene-transcription-and-yeast-response-to-cisplatin
#6
Ángel Vizoso-Vázquez, Mónica Lamas-Maceiras, M Isabel González-Siso, M Esperanza Cerdán
Ixr1 is a Saccharomyces cerevisiae HMGB protein that regulates the hypoxic regulon and also controls the expression of other genes involved in the oxidative stress response or re-adaptation of catabolic and anabolic fluxes when oxygen is limiting. Ixr1 also binds with high affinity to cisplatin-DNA adducts and modulates DNA repair. The influence of Ixr1 on transcription in the absence or presence of cisplatin has been analyzed in this work. Ixr1 regulates other transcriptional factors that respond to nutrient availability or extracellular and intracellular stress stimuli, some controlled by the TOR pathway and PKA signaling...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29448896/circulating-neutrophil-extracellular-traps-are-elevated-in-patients-with-deep-infiltrating-endometriosis
#7
Jordina Munrós, Dolors Tàssies, Joan Carles Reverter, Lidia Martin, Amelia Pérez, Francisco Carmona, María Ángeles Martínez-Zamora
Neutrophil extracellular traps (NETs) have been described to be related to the pathogenesis of inflammatory and autoimmune conditions. Endometriosis is currently considered a chronic inflammatory condition. Therefore, we performed a preliminary case-control study to compare the circulating plasma NET levels in patients with surgically confirmed endometriosis (E group, n = 82) and those of patients without surgical findings of endometriosis (C group, n = 35). Venous blood samples were obtained at the time of surgery...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29446804/t6ss-intraspecific-competition-orchestrates-vibrio-cholerae-genotypic-diversity
#8
REVIEW
Benjamin Kostiuk, Daniel Unterweger, Daniele Provenzano, Stefan Pukatzki
Vibrio cholerae is a diverse species that inhabits a wide range of environments from copepods in brackish water to the intestines of humans. In order to remain competitive, V. cholerae uses the versatile type-VI secretion system (T6SS) to secrete anti-prokaryotic and anti-eukaryotic effectors. In addition to competing with other bacterial species, V. cholerae strains also compete with one another. Some strains are able to coexist, and are referred to as belonging to the same compatibility group. Challenged by diverse competitors in various environments, different V...
September 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/29446800/biology-of-vibrio-cholera-editorial-overview
#9
EDITORIAL
Felipe Cava
In this monographic issue, we have the pleasure to present contributions from six of the leading laboratories at the forefront of Vibrio cholerae genetics, ecology and evolution, together with a brief tribute by Diego Romero to Doctor Jaime Ferrán y Clua, a pioneering Spanish bacteriologist who developed the first vaccine against this pathogen. V. cholerae is a free-living aquatic bacterium that interacts with and infects a variety of organisms. In humans it causes cholera, the deadly diarrhoea that was responsible for millions of deaths during seven pandemics since 1817, and still thousands every year...
September 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/29446183/comparison-of-detergent-based-decellularization-protocols-for-the-removal-of-antigenic-cellular-components-in-porcine-aortic-valve
#10
Xiaohong Liu, Ning Li, Dejun Gong, Cuiping Xia, Zhiyun Xu
BACKGROUND: Various detergent-based protocols are used to remove cells and cellular debris in porcine aortic valve (PAV). However, the removal of antigenic cellular components has not been thoroughly elucidated to date. In this study, we used 4 detergent-based protocols to decellularize PAVs and aimed to evaluate their effects on removing antigenic cellular components. METHODS: Porcine aortic valves were decellularized using sodium dodecyl sulfate (SDS), SDS in combination with sodium deoxycholate (SDS/SD), Triton X-100, and Triton X-100 in combination with SD (Triton X-100/SD), respectively...
February 15, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29445365/-babesia-microti-infection-changes-host-spleen-architecture-and-is-cleared-by-a-th1-immune-response
#11
Vitomir Djokic, Lavoisier Akoolo, Nikhat Parveen
Babesia microti is a malaria-like parasite, which infects ∼2000 people annually, such that babesiosis is now a notifiable disease in the United States. Immunocompetent individuals often remain asymptomatic and are tested only after they feel ill. Susceptible C3H/HeJ mice show several human-like disease manifestations and are ideal to study pathogenesis of Babesia species. In this study, we examined parasitemia of B. microti at different time points and assessed its impact on hemoglobin levels in blood, on spleen pathology and overall immune response in C3H/HeJ mice...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29444691/affinity-proteomic-profiling-of-plasma-for-proteins-associated-to-area-based-mammographic-breast-density
#12
Sanna Byström, Martin Eklund, Mun-Gwan Hong, Claudia Fredolini, Mikael Eriksson, Kamila Czene, Per Hall, Jochen M Schwenk, Marike Gabrielson
BACKGROUND: Mammographic breast density is one of the strongest risk factors for breast cancer, but molecular understanding of how breast density relates to cancer risk is less complete. Studies of proteins in blood plasma, possibly associated with mammographic density, are well-suited as these allow large-scale analyses and might shed light on the association between breast cancer and breast density. METHODS: Plasma samples from 1329 women in the Swedish KARMA project, without prior history of breast cancer, were profiled with antibody suspension bead array (SBA) assays...
February 14, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29443623/calprotectin-and-the-initiation-and-progression-of-head-and-neck-cancer
#13
P P Argyris, Z M Slama, K F Ross, A Khammanivong, M C Herzberg
Calprotectin (S100A8/A9), a heterodimeric complex of calcium-binding proteins S100A8 and S100A9, is encoded by genes mapping to the chromosomal locus 1q21.3 of the epidermal differentiation complex. Whereas extracellular calprotectin shows proinflammatory and antimicrobial properties by signaling through RAGE and TLR4, intracytoplasmic S100A8/A9 appears to be important for cellular development, maintenance, and survival. S100A8/A9 is constitutively expressed in myeloid cells and the stratified mucosal epithelia lining the oropharyngeal and genitourinary mucosae...
February 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29442894/graphene-oxide-incorporated-plga-nanofibrous-scaffold-for-solid-phase-gene-delivery-into-mesenchymal-stem-cells
#14
Zhiying Wang, He Shen, Saijie Song, Liming Zhang, Wei Chen, Jianwu Dai, Zhijun Zhang
Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in combination with biomaterial scaffolds, has raised increasing interests from researchers in tissue engineering. Nanofibers fabricated by electrospinning technique mimicking extracellular matrix (ECM) are widely used in tissue engineering applications...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442011/acetylpuerarin-protects-against-ogd-induced-cell-injury-in-bv2-microglia-by-inhibiting-hmgb1-release
#15
Deqing Sun, Aiying Xue, Xia Xue, Manru Ren, Jing Wu, Rongmei Wang
High mobility group box 1 (HMGB1), a non-histone DNA-binding protein, is massively released into the extracellular space from neuronal cells after ischemic injury, initiates inflammatory response and aggravates brain tissue damage. Acetylpuerarin (AP), an acetylated derivative of puerarin, was reported to protect against cerebrovascular ischemia-reperfusion injury in rats through anti-inflammation. In the present study, we aim to investigate whether AP inhibited HMGB1 release in oxygen-glucose deprivation (OGD)-treated BV2 microglia...
February 1, 2018: Die Pharmazie
https://www.readbyqxmd.com/read/29441415/novel-approach-to-study-gastropod-mediated-innate-immune-reactions-against-metastrongyloid-parasites
#16
Felipe Penagos-Tabares, Malin K Lange, Anika Seipp, Ulrich Gärtner, Helena Mejer, Anja Taubert, Carlos Hermosilla
The anthropozoonotic metastrongyloid nematodes Angiostrongylus cantonensis and Angiostrongylus costaricensis, as well as Angiostrongylus vasorum, Crenosoma vulpis, Aelurostrongylus abstrusus and Troglostrongylus brevior are currently considered as emerging gastropod-borne parasites and have gained growing scientific attention in the last years. However, the knowledge on invertebrate immune responses and on how metastrongyloid larvae are attacked by gastropod immune cells is still limited. This work aims to describe an in vitro system to investigate haemocyte-derived innate immune responses of terrestrial gastropods induced by vital axenic metastrongyloid larvae...
February 13, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29438080/thrombus-neutrophil-extracellular-traps-content-impair-tpa-induced-thrombolysis-in-acute-ischemic-stroke
#17
Celina Ducroux, Lucas Di Meglio, Stephane Loyau, Sandrine Delbosc, William Boisseau, Catherine Deschildre, Malek Ben Maacha, Raphael Blanc, Hocine Redjem, Gabriele Ciccio, Stanislas Smajda, Robert Fahed, Jean-Baptiste Michel, Michel Piotin, Laurence Salomon, Mikael Mazighi, Benoit Ho-Tin-Noe, Jean-Philippe Desilles
BACKGROUND AND PURPOSE: Neutrophil Extracellular Traps (NETs) are DNA extracellular networks decorated with histones and granular proteins produced by activated neutrophils. NETs have been identified as major triggers and structural factors of thrombosis. A recent study designated extracellular DNA threads from NETs as a potential therapeutic target for improving tissue-type plasminogen activator (tPA)-induced thrombolysis in acute coronary syndrome. The aim of this study was to assess the presence of NETs in thrombi retrieved during endovascular therapy in patients with acute ischemic stroke (AIS) and their impact on tPA-induced thrombolysis...
February 8, 2018: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/29434999/functions-of-nuclear-actin-binding-proteins-in-human-cancer
#18
Xinyi Yang, Ying Lin
Nuclear actin-binding proteins (ABPs) perform distinguishable functions compared with their cytoplasmic counterparts in extensive activities of living cells. In addition to the ability to regulate actin cytoskeleton dynamics, nuclear ABPs are associated with multiple nuclear biological processes, including chromatin remodeling, gene transcriptional regulation, DNA damage response, nucleocytoplasmic trafficking and nuclear structure maintenance. The nuclear translocation of ABPs is affected by numerous intracellular or extracellular stimuli, which may lead to developmental malformation, tumor initiation, tumor progression and metastasis...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434871/mechanisms-for-dna-damaging-agent-induced-inactivation-of-erbb2-and-erbb3-via-the-erk-and-p38-signaling-pathways
#19
Chul Min Park, Yuki Kawasaki, Alaa Refaat, Hiroaki Sakurai
Cisplatin (CDDP) and doxorubicin (DOX) are chemotherapeutic drugs that trigger apoptosis by inducing DNA-damage. A previous study using breast cancer cells demonstrated the negative feedback modulation of the epidermal growth factor receptor (EGFR) and receptor tyrosine-protein kinase erbB-2 (ErbB2) via extracellular signal-regulated kinase (ERK)-mediated phosphorylation of conserved Thr-669 and Thr-677 residues, respectively, in the juxtamembrane domain. In addition, CDDP has been identified to cause negative feedback inhibition of activated EGFR in lung cancer cells...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434868/cytotoxic-effect-of-efavirenz-in-bxpc-3-pancreatic-cancer-cells-is-based-on-oxidative-stress-and-is-synergistic-with-ionizing-radiation
#20
Markus Hecht, Thomas Harrer, Verena Körber, Eric O Sarpong, Fabian Moser, Nora Fiebig, Manuela Schwegler, Michael Stürzl, Rainer Fietkau, Luitpold V Distel
The non-nucleoside reverse transcriptase inhibitor (NNRTI) Efavirenz is frequently used in human immunodeficiency virus treatment, but also efficient against cancer in mouse models. Its radiosensitizing effect makes it a promising drug for combination with radiotherapy. The efficacy of Efavirenz combined with irradiation was assessed with immunostaining of DNA-damage markers and colony formation assays in BxPC-3 pancreatic cancer cells. Gene expression and protein phosphorylation of the Efavirenz-sensitive BxPC-3 cells was compared to the resistant primary fibroblasts SBL-5...
February 2018: Oncology Letters
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