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https://www.readbyqxmd.com/read/29691795/improved-protocol-for-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells-effect-of-pthrp-and-fgf-2-on-tgf%C3%AE-1-bmp2-induced-chondrocytes-hypertrophy
#1
Davood Nasrabadi, Siamak Rezaeiani, Mohamadreza Baghaban Eslaminejad, Aliakbar Shabani
Growth factors have a pivotal role in chondrogenic differentiation of stem cells. The differential effects of known growth factors involved in the maintenance and homeostasis of cartilage tissue have been previously studied in vitro. However, there are few reported researches about the interactional effects of growth factors on chondrogenic differentiation of stem cells. The aim of this study is to examine the combined effects of four key growth factors on chondrogenic differentiation of mesenchymal stem cells (MSCs)...
April 24, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29688404/heparan-sulfate-proteoglycan-sulfation-regulates-uterine-differentiation-and-signaling-during-embryo-implantation
#2
Yan Yin, Adam Wang, Li Feng, Yu Wang, Hong Zhang, Ivy Zhang, Brent Bany, Liang Ma
To prepare for embryo implantation, uterus must undergo a series of reciprocal interactions between the uterine epithelium and the underlying stroma, which are orchestrated by ovarian hormones. During this process, multiple signaling pathways are activated to direct cell proliferation and differentiation, which render the uterus receptive to the implanting blastocysts. One important modulator of these signaling pathways is the cell surface and extracellular matrix macromolecules, heparan sulfate proteoglycans (HSPGs)...
April 23, 2018: Endocrinology
https://www.readbyqxmd.com/read/29684814/transcriptional-and-translational-abundance-of-bone-morphogenetic-protein-bmp-2-4-6-7-and-their-receptors-bmpr1a-1b-and-bmpr2-in-buffalo-ovarian-follicle-and-the-role-of-bmp4-and-bmp7-on-estrogen-production-and-survival-of-cultured-granulosa-cells
#3
G Rajesh, S R Mishra, A Paul, M Punetha, G M Vidyalakshmi, K Narayanan, S Bag, S K Bhure, V Singh Chouhan, V P Maurya, G Singh, Mihir Sarkar
BMPs and their receptors modulate the granulosa cell (GC) function in the follicle of domestic animals. Since little is known on BMPs in the buffalo, the present study was aimed to investigate the expression of BMP2, 4, 6, 7 and their receptors BMPR1A, BMPR1B, BMPR2 in the GC and theca cells (TC) of ovarian follicles and the role of BMP4 and BMP7 on buffalo GC. Follicles were classified into four groups based on size and E2 level in the follicular fluid as follows: (i) Group1(4-6 mm; <0.5 ng/mL) (ii) Group 2 (7-9 mm; 0...
April 16, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29678674/the-role-of-the-micro-pattern-and-nano-topography-of-hydroxyapatite-bioceramics-on-stimulating-osteogenic-differentiation-of-mesenchymal-stem-cells
#4
Cancan Zhao, Xiaoya Wang, Long Gao, Linguo Jing, Quan Zhou, Jiang Chang
The micro/nano hybrid structure is considered to be a biomaterial characteristic to stimulate osteogenesis by mimicking the three-dimensional structure of the bone matrix. However, the mechanism of the hybrid structure induced osteogenic differentiation of stem cells is still unknown. For elucidating the mechanisms, one of the challenge is to directly fabricate micro/nano hybrid structure on bioceramics because of its brittleness. In this study, hydroxyapatite (HA) bioceramics with the micro/nano hybrid structure were firstly fabricated via a hydrothermal treatment and template method, and the effect of the different surface structures on the expression of integrins, BMP2 signaling pathways and cell-cell communication was investigated...
April 17, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29677546/bacitracin-promotes-osteogenic-differentiation-of-human-bone-marrow-mesenchymal-stem-cells-by-stimulating-the-bone-morphogenetic-protein-2-smad-axis
#5
Hui Li, Bin'en Nie, Zhe Du, Shutao Zhang, Teng Long, Bing Yue
Bacitracin, a widely used metallopeptide antibiotic, has been reported to be locally used in treating wounds without systemic adverse reactions. Our preliminary study showed that bacitracin might enhance the osteogenic differentiation of human bone marrow mesenchymal stem cells (HBMSCs). The present study investigated whether bacitracin affects the osteogenic differentiation of HBMSCs and the molecular mechanisms involved. The proliferation of HBMSCs in the presence of bacitracin was examined using a cell counting kit-8 (CCK-8) assay...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29677202/%C3%AE-1-integrins-mediate-the-bmp2-dependent-transcriptional-control-of-osteoblast-differentiation-and-osteogenesis
#6
Molly Brunner, Noémie Mandier, Thierry Gautier, Genevieve Chevalier, Anne-Sophie Ribba, Philippe Guardiola, Marc R Block, Daniel Bouvard
Osteoblast differentiation is a highly regulated process that requires coordinated information from both soluble factors and the extracellular matrix. Among these extracellular stimuli, chemical and physical properties of the matrix are sensed through cell surface receptors such as integrins and transmitted into the nucleus to drive specific gene expression. Here, we showed that the conditional deletion of β1 integrins in the osteo-precursor population severely impacts bone formation and homeostasis both in vivo and in vitro...
2018: PloS One
https://www.readbyqxmd.com/read/29673055/application-of-mesenchymal-stem-cells-to-enhance-non-union-bone-fracture-healing
#7
Mohammad Mousaei Ghasroldasht, Maryam M Matin, Hossein Kazemi Mehrjerdi, Hojjat Naderi-Meshkin, Ali Moradi, Masoud Rajabioun, Faeze Alipour, Samaneh Ghasemi, Mohammad Zare, Mahdi Mirahmadi, Hamid Reza Bidkhori, Ahmad Reza Bahrami
ECM components include a number of osteoinductive and osteoconductive factors, which are involved in bone fracture healing. In this study, a combination of adipose derived mesenchymal stem cells (Ad-MSCs), cancellous bone graft (CBG), and chitosan hydrogel (CHI) was applied to the in non-union bone fracture and healing effects were evaluated for the first time. After creation of animal models with non-union fracture in rats, they were randomly classified into seven groups. Radiography at 0, 2, 4 and 8 weeks after surgery, indicated the positive effects of Ad-MSCs+CBG+CHI and Ad-MSCs+CBG in treatment of bone fractures as early as 2 weeks after the surgery...
April 19, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29672339/the-effects-of-episodic-alcohol-exposure-on-bmp2-signaling-during-tibia-fracture-healing
#8
Anthony Bratton, Joshua Eisenberg, Aleksandra Vuchkovska, Philip Roper, John J Callaci
OBJECTIVES: To explore how alcohol affects the BMP-2 signaling pathway, which is known to play a critical role in bone and cartilage formation during fracture healing. METHODS: A rat model was used to demonstrate the detrimental effects of alcohol exposure on tibia fracture healing. Specific components of the BMP-2 pathway were analyzed in fracture callus at days 3, 7, 14, 21 post fracture via western immunoassays and ELISA. RESULTS: Alcohol exposure prior to tibia fracture demonstrated attenuation of downstream BMP-2 signaling...
April 18, 2018: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29665134/the-role-of-bmp2-in-the-maturation-and-maintenance-of-the-murine-knee-joint
#9
Laura W Gamer, Steven Pregizer, Jackson Gamer, Marina Feigenson, Andreia Ionescu, Qing Li, Lin Han, Vicki Rosen
Bone morphogenetic proteins (BMPs) are key regulators of skeletal development, growth and repair. While BMP signaling is required for synovial joint formation and is also involved in preserving joint function after birth, the role of specific BMP ligands in adult joint homeostasis remains unclear. The purpose of this study was to define the role of Bmp2 in the morphogenesis and maintenance of the knee joint. To do this, we first created Bmp2-LacZ and Gdf5-LacZ knock-in mice and compared their expression patterns in the developing and postnatal murine knee joint...
April 17, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29663368/a-peptide-that-blocks-the-interaction-of-nf-%C3%AE%C2%BAb-p65-subunit-with-smad4-enhances-bmp2-induced-osteogenesis
#10
Mariko Urata, Shoichiro Kokabu, Takuma Matsubara, Goro Sugiyama, Chihiro Nakatomi, Hiroshi Takeuchi, Shizu Hirata-Tsuchiya, Kazuhiro Aoki, Yukihiko Tamura, Yasuko Moriyama, Yasunori Ayukawa, Miho Matsuda, Min Zhang, Kiyoshi Koyano, Chiaki Kitamura, Eijiro Jimi
Bone morphogenetic protein (BMP) potentiates bone formation through the Smad signaling pathway in vitro and in vivo. The transcription factor nuclear factor κB (NF-κB) suppresses BMP-induced osteoblast differentiation. Recently, we identified that the transactivation (TA) 2 domain of p65, a main subunit of NF-κB, interacts with the mad homology (MH) 1 domain of Smad4 to inhibit BMP signaling. Therefore, we further attempted to identify the interacting regions of these two molecules at the amino acid level...
April 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29662780/distinct-metabolic-programs-induced-by-tgf-%C3%AE-1-and-bmp2-in-human-articular-chondrocytes-with-osteoarthritis
#11
Cuicui Wang, Richard M Silverman, Jie Shen, Regis J O'Keefe
Objectives: Cellular energy metabolism is important for the function of all tissues, including cartilage. Recent studies indicate that superficial and deep subpopulations of articular chondrocytes (ACs) have distinct metabolic profiles. At the cellular and molecular level, osteoarthritis (OA) is characterised by alteration from a healthy homoeostatic state towards a catabolic state. Several molecular pathways, including transforming growth factor beta (TGF-β) and bone morphogenetic protein (BMP) signalling, have been identified as critical players in the pathogenesis and progression of OA...
January 2018: Journal of Orthopaedic Translation
https://www.readbyqxmd.com/read/29658921/site-directed-immobilization-of-bone-morphogenetic-protein-2-to-solid-surfaces-by-click-chemistry
#12
Claudia Siverino, Barbara Tabisz, Tessa Lühmann, Lorenz Meinel, Thomas Müller, Heike Walles, Joachim Nickel
Different therapeutic strategies for the treatment of non-healing long bone defects have been intensively investigated. Currently used treatments present several limitations that have led to the use of biomaterials in combination with osteogenic growth factors, such as bone morphogenetic proteins (BMPs). Commonly used absorption or encapsulation methods require supra-physiological amounts of BMP2, typically resulting in a so-called initial burst release effect that provokes several severe adverse side effects...
March 29, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29651874/in-vitro-and-in-vivo-evaluation-of-osteoinductivity-and-bone-fusion-ability-of-an-activin-a-bmp2-chimera-ab204-a-comparison-study-between-ab204-and-rhbmp-2
#13
Guang Bin Zheng, Jae Hyup Lee, Yuan-Zhe Jin
This study compared osteoinductivity and osteogenic capacity between AB204 and rhBMP-2 using hMSCs in vitro and a beagle's posterolateral spinal fusion model. Cultured hMSCs were treated with AB204 or rhBMP-2 with low to high doses. Three male beagles were performed posterolateral spinal fusion with biphasic calcium phosphate (2 ml) + AB204 or rhBMP-2 (20, 50 or 200 µg). They were euthanized after 8 weeks. The fusion rate and bone formation of spine samples were examined. AB204 had higher alkaline phosphatase activity, mineralization and osteogenic-related gene expression than rhBMP-2...
April 13, 2018: Growth Factors
https://www.readbyqxmd.com/read/29649529/mesoporous-silicate-nanoparticles-3d-nanofibrous-scaffold-mediated-dual-drug-delivery-for-bone-tissue-engineering
#14
Qingqing Yao, Yangxi Liu, Balaranjan Selvaratnam, Ranjit T Koodali, Hongli Sun
Controlled delivery systems play a critical role in the success of bone morphogenetic proteins (i.e., BMP2 and BMP7) for challenged bone repair. Instead of single-drug release that is currently and commonly prevalent, dual-drug delivery strategies are highly desired to achieve effective bone regeneration because natural bone repair process is driven by multiple factors. Particularly, angiogenesis is essential for osteogenesis and requires more than just one factor (e.g., Vascular Endothelial Growth Factor, VEGF)...
April 9, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29643386/somatic-activating-mutations-in-map2k1-cause-melorheostosis
#15
Heeseog Kang, Smita Jha, Zuoming Deng, Nadja Fratzl-Zelman, Wayne A Cabral, Aleksandra Ivovic, Françoise Meylan, Eric P Hanson, Eileen Lange, James Katz, Paul Roschger, Klaus Klaushofer, Edward W Cowen, Richard M Siegel, Joan C Marini, Timothy Bhattacharyya
Melorheostosis is a sporadic disease of uncertain etiology characterized by asymmetric bone overgrowth and functional impairment. Using whole exome sequencing, we identify somatic mosaic MAP2K1 mutations in affected, but not unaffected, bone of eight unrelated patients with melorheostosis. The activating mutations (Q56P, K57E and K57N) cluster tightly in the MEK1 negative regulatory domain. Affected bone displays a mosaic pattern of increased p-ERK1/2 in osteoblast immunohistochemistry. Osteoblasts cultured from affected bone comprise two populations with distinct p-ERK1/2 levels by flow cytometry, enhanced ERK1/2 activation, and increased cell proliferation...
April 11, 2018: Nature Communications
https://www.readbyqxmd.com/read/29627497/osteoblast-secreted-wisp-1-promotes-adherence-of-prostate-cancer-cells-to-bone-via-the-vcam-1-integrin-%C3%AE-4%C3%AE-1-system
#16
An-Chen Chang, Po-Chun Chen, Yu-Feng Lin, Chen-Ming Su, Ju-Fang Liu, Tien-Huang Lin, Show-Mei Chuang, Chih-Hsin Tang
Bone metastasis is a frequent occurrence in prostate cancer (PCa) that is associated with severe complications such as fracture, bone pain and hypercalcemia. The cross-talk between metastatic cancer cells and bone is critical to the development and progression of bone metastases. In our previous data, we have described how the involvement of the Wnt-induced secreted protein-1/vascular cell adhesion molecule-1 (WISP-1/VCAM-1) system in this tumor-bone interaction contributes to human PCa cell motility. In this study, we found that WISP-1 regulates bone mineralization by inducing bone morphogenetic protein-2 (BMP2), BMP4 and osteopontin (OPN) expression in osteoblasts...
April 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29622677/heparan-sulfate-antagonism-alters-bone-morphogenetic-protein-signaling-and-receptor-dynamics-suggesting-a-mechanism-in-hereditary-multiple-exostoses
#17
Christina Mundy, Evan Yang, Hajime Takano, Paul C Billings, Maurizio Pacifici
Hereditary Multiple Exostoses (HME) is a pediatric disorder caused by heparan sulfate (HS) deficiency and is characterized by growth plate-associated osteochondromas. Previously, we found that osteochondroma formation in mouse models is preceded by ectopic bone morphogenetic protein (BMP) signaling in the perichondrium, but the mechanistic relationships between BMP signaling and HS deficiency remain unclear. Therefore, we used an HS antagonist (Surfen) to investigate the effects of this HS interference on BMP signaling, ligand availability, cell surface BMP receptor (BMPR) dynamics and BMPR interactions in Ad-293 and C3H/10T1/2 cells...
April 5, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29581780/switch-of-the-ovarian-cancer-cell-to-a-calcifying-phenotype-in-the-calcification-of-ovarian-cancer
#18
Jirui Wen, Zhiwei Zhao, Liwei Huang, Liang Li, Jiman Li, Ye Zeng, Jiang Wu, Yali Miao
OBJECTIVE: The main aim of this study was to study swith of the ovarian cancer cell to a calcifying phenotype in the formation of calcification in ovarian cancer, and to offer some help for ovarian cancer's diagnosis and differentiation therapy. METHODS: The mineralization of ovarian cancer cell lines SKOV3 was induced via calcification medium for 21 d in vitro . Alizarin red staining, von kossa staining, calcein fluorescence staining and ALP activity detection were used to identify mineralization in calcification model of ovarian cancer...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29577064/functionalization-of-pcl-3d-electrospun-nanofibrous-scaffolds-for-improved-bmp2-induced-bone-formation
#19
Jacob M Miszuk, Tao Xu, Qingqing Yao, Fang Fang, Josh D Childs, Zhongkui Hong, Jianning Tao, Hao Fong, Hongli Sun
Bone morphogenic protein 2 (BMP2) is a key growth factor for bone regeneration, possessing FDA approval for orthopedic applications. BMP2 is often required in supratherapeutic doses clinically, yielding adverse side effects and substantial treatment costs. Considering the crucial role of materials for BMPs delivery and cell osteogenic differentiation, we devote to engineering an innovative bone-matrix mimicking niche to improve low dose of BMP2-induced bone formation. Our previous work describes a novel technique, named thermally induced nanofiber self-agglomeration (TISA), for generating 3D electrospun nanofibrous (NF) polycaprolactone (PCL) scaffolds...
March 2018: Applied Materials Today
https://www.readbyqxmd.com/read/29574511/small-ubiquitin-like-modifier-protein-3-enhances-the-solubilization-of-human-bone-morphogenetic-protein-2-in-e-coli
#20
Muhammad Umair Hanif, Adnan Yaseen, Roquyya Gul, Muhammad Usman Mirza, Muhammad Hassan Nawaz, Syed Shoaib Ahmed, Salman Aziz, Saima Chaudhary, Ayyaz Ali Khan, Muhammad Shoaib
Small ubiquitin-like modifier (SUMO) fusion technology is widely used in the production of heterologous proteins from prokaryotic system to aid in protein solubilization and refolding. Due to an extensive clinical application of human bone morphogenetic protein 2 (hBMP2) in bone augmentation, total RNA was isolated from human gingival tissue and mature gene was amplified through RT-PCR, cloned (pET21a), sequence analyzed, and submitted to GenBank (Accession no. KF250425). To obtain soluble expression, SUMO3 was tagged at the N-terminus of hBMP2 gene (pET21a/SUMO3-hBMP2), transferred in BL21 codon+, and ~ 40% soluble expression was obtained on induction with IPTG...
March 24, 2018: Applied Biochemistry and Biotechnology
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