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https://www.readbyqxmd.com/read/29052311/functionalizing-titanium-surface-with-pamam-dendrimer-and-human-bmp2-gene-via-layer-by-layer-assembly-for-enhanced-osteogenesis-%C3%A2
#1
Weizhen Chen, Wen Li, Kui Xu, Menghuan Li, Liangliang Dai, Xinkun Shen, Yan Hu, Kaiyong Cai
The study reports on the gene-functionalization of titanium substrates with multilayered functional human BMP2 (hBMP2) gene plasmid and its effects on osteogenesis in vitro and in vivo. The multilayers comprising cationic poly (amidoamine) (PAMAM) dendrimer/EGFP-hBMP2 plasmid DNA complex (d-DNA) and anionic naked plasmid were deposited on titanium substrates via layer-by-layer (LbL) assembly technique, which was revealed by atomic force microscopy (AFM), water contact angle measurement and release profiles...
October 20, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29050936/t%C3%AE-riii-is-induced-by-tcr-signaling-and-downregulated-in-foxp3-regulatory-t-cellsm
#2
Sandra Ortega-Francisco, Marisol de la Fuente-Granada, Evelyn K Alvarez-Salazar, Lizbeth Airais Bolaños-Castro, Gabriela Fonseca Camarillo, Roxana Olguin Alor, Germán R Alemán-Muench, Fernando López-Casillas, Chander Raman, Eduardo A García-Zepeda, Gloria Soldevila
TGF-β type III receptor (TβRIII) is a co-receptor for TGFβ family members required for high-affinity binding of these ligands to their receptors, potentiating their cellular functions. TGF-βs, Bone Morphogenetic Proteins (BMP2/4) and Inhibins/Activins regulate different checkpoints during T cell differentiation. We have previously reported that TβRIII modulates T cell development by protecting developing thymocytes from apoptosis, however the role of this co-receptor in peripheral lymphocytes still remains elusive...
October 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29046696/early-changes-in-retinal-structure-and-bmp2-expression-in-the-retina-and-crystalline-lens-of-streptozotocin-induced-diabetic-pigs
#3
Jae Seung Jeong, Woon-Kyu Lee, Yeon Sung Moon, Na Rae Kim
PURPOSE: This study aims to evaluate early changes in retinal structure and BMP2 expression in the retina and crystalline lens by comparing streptozotocin-induced diabetic pigs and normal control group pigs. METHODS: Five eye samples from five diabetic Micro-pigs (Medikinetics, Pyeongtaek, Korea) and five eye samples from five control pigs bred in a specific pathogen-free area were used. Diabetes was developed through intravenous injection of nicotinamide and streptozotocin, and the average fasting glucose level was maintained at 250 mg/dL or higher for 16 weeks...
September 2017: Laboratory Animal Research
https://www.readbyqxmd.com/read/29045937/adenovirus-mediated-expression-of-bmp-2-and-bfgf-in-bone-marrow-mesenchymal-stem-cells-combined-with-demineralized-bone-matrix-for-repair-of-femoral-head-osteonecrosis-in-beagle-dogs
#4
Wu-Xun Peng, Lei Wang
BACKGROUND: This study investigated the effect of using adenovirus-mediated expression of bone morphogenetic protein 2 (Ad-BMP-2) and basic fibroblast growth factor (bFGF) in bone marrow mesenchymal stem cells (BMSCs) in combination with a demineralized bone matrix (DBM) to repair osteonecrosis of the femoral head (ONFH) in Beagle dogs. METHODS: A total of 30 Beagle dogs were selected for the isolation of BMSCs, which were cultured and transfected with the recombinant adenovirus vector Ad-BMP2-bFGF-GFP (carrying BMP-2 and bFGF) or a control adenovirus plasmid (encoding green fluorescent protein (Ad-GFP))...
October 18, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040148/long-term-effects-of-bone-morphogenetic-protein-2-loaded-calcium-phosphate-on-maxillary-sinus-lift-surgery-for-delayed-and-simultaneous-dental-implantation
#5
Yuefeng Ding, Xuejuan Wang
Maxillary sinus lift surgery (MSLS) is considered to be a useful treatment method for patients with atrophic alveolar bone. Choosing a suitable surgical procedure to simultaneously decrease graft absorption and obtain long-term survival of dental implants is still a challenge. In this study, 20 patients received MSLS with graft of bone morphogenetic protein 2 (BMP2)-loaded calcium phosphate. Dental implants were placed simultaneously in 10 patients receiving MSLS (1-stage group), and in 10 patients receiving dental implants with a 3 to 6 months delay (2-stage group)...
October 16, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29038835/interaction-between-lrp5-and-periostin-gene-polymorphisms-on-serum-periostin-levels-and-cortical-bone-microstructure
#6
J Pepe, N Bonnet, F R Herrmann, E Biver, R Rizzoli, T Chevalley, S L Ferrari
We investigated the interaction between periostin SNPs and the SNPs of the genes assumed to modulate serum periostin levels and bone microstructure in a cohort of postmenopausal women. We identified an interaction between LRP5 SNP rs648438 and periostin SNP rs9547970 on serum periostin levels and on radial cortical porosity. PURPOSE: The purpose of this study is to investigate the interaction between periostin gene polymorphisms (SNPs) and other genes potentially responsible for modulating serum periostin levels and bone microstructure in a cohort of postmenopausal women...
October 16, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/29029772/inhibition-of-mammalian-target-of-rapamycin-signaling-with-rapamycin-prevents-trauma-induced-heterotopic-ossification
#7
Ammar T Qureshi, Devaveena Dey, Erin M Sanders, Jonathan G Seavey, Allison M Tomasino, Kaitlyn Moss, Benjamin Wheatley, David Cholok, Shawn Loder, John Li, Benjamin Levi, Thomas A Davis
A pressing clinical need exists for 63% to 65% of combat-wounded service members and 11% to 20% of civilians who develop heterotopic ossification (HO) after blast-related extremity injury and traumatic injuries, respectively. The mammalian target of rapamycin pathway is a central cellular sensor of injury. We evaluated the prophylactic effects of rapamycin, a selective inhibitor of mammalian target of rapamycin signaling, on HO formation in a rat model of blast-related, polytraumatic extremity injury. Rapamycin was administered intraperitoneally daily for 14 days at 0...
October 5, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29028819/the-anti-myeloma-activity-of-bone-morphogenetic-protein-2-predominantly-relies-on-the-induction-of-growth-arrest-and-is-apoptosis-independent
#8
Charlotte Lagler, Mohamed El-Mesery, Alexander Christian Kübler, Urs Dietmar Achim Müller-Richter, Thorsten Stühmer, Joachim Nickel, Thomas Dieter Müller, Harald Wajant, Axel Seher
Multiple myeloma (MM), a malignancy of the bone marrow, is characterized by a pathological increase in antibody-producing plasma cells and an increase in immunoglobulins (plasmacytosis). In recent years, bone morphogenetic proteins (BMPs) have been reported to be activators of apoptotic cell death in neoplastic B cells in MM. Here, we use bone morphogenetic protein 2 (BMP2) to show that the "apoptotic" effect of BMPs on human neoplastic B cells is dominated by anti-proliferative activities and cell cycle arrest and is apoptosis-independent...
2017: PloS One
https://www.readbyqxmd.com/read/29019082/loss-of-the-nutrient-sensor-tas1r3-leads-to-reduced-bone-resorption
#9
Michael S Eaton, Nicholas Weinstein, Jordan B Newby, Maggie M Plattes, Hanna E Foster, Jon W Arthur, Taylor D Ward, Stephen R Shively, Ryann Shor, Justin Nathan, Hannah M Davis, Lilian I Plotkin, Eric M Wauson, Brian J Dewar, Aaron Broege, Jonathan W Lowery
The taste receptor type 1 (TAS1R) family of heterotrimeric G protein-coupled receptors participates in monitoring energy and nutrient status. TAS1R member 3 (TAS1R3) is a bi-functional protein that recognizes amino acids such as L-glycine and L-glutamate or sweet molecules such as sucrose and fructose when dimerized with TAS1R member 1 (TAS1R1) or TAS1R member 2 (TAS1R2), respectively. It was recently reported that deletion of TAS1R3 expression in Tas1R3 mutant mice leads to increased cortical bone mass but the underlying cellular mechanism leading to this phenotype remains unclear...
October 10, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/29018836/one-step-fabrication-of-bone-morphogenetic-protein-2-gene-activated-porous-poly-l-lactide-scaffold-for-bone-induction
#10
Jingwen Xue, Hang Lin, Allison Bean, Ying Tang, Jian Tan, Rocky S Tuan, Bing Wang
Bone morphogenetic protein 2 (BMP2) is an efficacious inducer for the osteogenesis of mesenchymal stem cells (MSCs). Conventional applications of BMP2 have involved either the direct incorporation of BMP2 protein or ex vivo BMP2 gene transfer into stem cells prior to their transplantation. These approaches are able to promote bone formation to some extent; however, they are hampered by either the lack of stability and sustainability of BMP2 protein or the time-consuming and cost-prohibitive in vitro cell culture procedure...
December 15, 2017: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29017973/modulating-microfibrillar-alignment-and-growth-factor-stimulation-to-regulate-mesenchymal-stem-cell-differentiation
#11
Dinorath Olvera, Binulal N Sathy, Simon F Carroll, Daniel J Kelly
The ideal tissue engineering (TE) strategy for ligament regeneration should recapitulate the bone - calcified cartilage - fibrocartilage - soft tissue interface. Aligned electrospun-fibers have been shown to guide the deposition of a highly organized extracellular matrix (ECM) necessary for ligament TE. However, recapitulating the different tissues observed in the bone-ligament interface using such constructs remains a challenge. This study aimed to explore how fiber alignment and growth factor stimulation interact to regulate the chondrogenic and ligamentous differentiation of mesenchymal stem cells (MSCs)...
October 7, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28991390/magnetically-controlled-growth-factor-immobilized-multilayer-cell-sheets-for-complex-tissue-regeneration
#12
Wenjie Zhang, Guangzheng Yang, Xiansong Wang, Liting Jiang, Fei Jiang, Guanglong Li, Zhiyuan Zhang, Xinquan Jiang
The scaffold-free cell-sheet technique plays a significant role in stem-cell-based regeneration. Furthermore, growth factors are known to direct stem cell differentiation and enhance tissue regeneration. However, the absence of an effective means to incorporate growth factors into the cell sheets hinders further optimization of the regeneration efficiency. Here, a novel design of magnetically controlled "growth-factor-immobilized cell sheets" is reported. A new Fe3 O4 magnetic nanoparticle (MNP) coated with nanoscale graphene oxide (nGO@Fe3 O4 ) is developed to label stem cells and deliver growth factors...
October 9, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28988712/antimicrobial-peptide-combined-with-bmp2-modified-mesenchymal-stem-cells-promotes-calvarial-repair-in-an-osteolytic-model
#13
Zunpeng Liu, Xue Yuan, Min Liu, Gabriela Fernandes, Yejia Zhang, Shuting Yang, Ciprian N Ionita, Shuying Yang
Repair and regeneration of inflammation-induced bone loss remains a clinical challenge. LL37, an antimicrobial peptide, plays critical roles in cell migration, cytokine production, apoptosis, and angiogenesis. Migration of stem cells to the affected site and promotion of vascularization are essential for tissue engineering therapy, including bone regeneration. However, it is largely unknown whether LL37 affects mesenchymal stem cell (MSC) behavior and bone morphogenetic protein 2 (BMP2)-mediated bone repair during the bone pathologic remodeling process...
September 14, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28985029/a-variant-associated-with-sagittal-nonsyndromic-craniosynostosis-alters-the-regulatory-function-of-a-non-coding-element
#14
Cristina M Justice, Jinoh Kim, Sun-Don Kim, Kyunhgho Kim, Garima Yagnik, Araceli Cuellar, Blake Carrington, Chung-Ling Lu, Raman Sood, Simeon A Boyadjiev, Alexander F Wilson
Craniosynostosis presents either as a nonsyndromic congenital anomaly or as a finding in nearly 200 genetic syndromes. Our previous genome-wide association study of sagittal nonsyndromic craniosynostosis identified associations with variants downstream from BMP2 and intronic in BBS9. Because no coding variants in BMP2 were identified, we hypothesized that conserved non-coding regulatory elements may alter BMP2 expression. In order to identify and characterize noncoding regulatory elements near BMP2, two conserved noncoding regions near the associated region on chromosome 20 were tested for regulatory activity with a Renilla luciferase assay...
October 6, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28982588/low-1-level-mechanical-vibration-improves-bone-microstructure-tissue-mechanical-properties-and-porous-titanium-implant-osseointegration-by-promoting-anabolic-response-in-type-1-diabetic-rabbits
#15
Da Jing, Zedong Yan, Jing Cai, Shichao Tong, Xiaokang Li, Zheng Guo, Erping Luo
Type 1 diabetes mellitus (T1DM) is associated with reduced bone mass, increased fracture risk, and impaired bone defect regeneration potential. These skeletal complications are becoming important clinical challenges due to the rapidly increasing T1DM population, which necessitates developing effective treatment for T1DM-associated osteopenia/osteoporosis and bone trauma. This study aims to investigate the effects of whole-body vibration (WBV), an easy and non-invasive biophysical method, on bone microstructure, tissue-level mechanical properties and porous titanium (pTi) osseointegration in alloxan-diabetic rabbits...
October 2, 2017: Bone
https://www.readbyqxmd.com/read/28980278/do-we-need-retarded-delivery-of-bone-growth-factors-in-facial-bone-repair-an-experimental-study-in-rats
#16
N Moser, N Lohse, J Golstein, P Kauffmann, B Sven, M Epple, H Schliephake
The aim of the present study was to evaluate the effect of different dosages of retarded vs. rapid release of bone morphogenic protein 2 (BMP2) at different recipient sites. Porous composite poly(D,L-lactic acid) (PDLLA)/CaCO3 scaffolds were loaded with three different dosages of rhBMP2 (24 µg, 48 µg and 96 µg) and implanted, together with blank controls, both into non-healing defects of the mandibles and into the gluteal muscles of 24 adult male Wistar rats. After 26 weeks, bone formation and expression of bone specific markers [alkaline phosphatase (AP) and Runx2] were evaluated by histomorphometry and immunohistochemistry...
October 5, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28980049/effects-of-endogenous-hypercortisolism-on-bone-mrna-and-microrna-expression-in-humans
#17
Z E Belaya, T A Grebennikova, G A Melnichenko, A G Nikitin, A G Solodovnikov, O I Brovkina, A U Grigoriev, L Y Rozhinskaya, I I Dedov
Hypercortisolism in humans suppresses osteoblastogenesis and osteoblast function through the upregulation of Wnt-signaling antagonists (sclerostin, Dkk1) and changes in microRNAs levels (miR-125b-5p, miR-218-5p, miR-34a-5p, miR-188-3p, miR-199a-5p) which are associated with mesenchymal stem-cell commitment to adipocytes or cartilage cells over the osteoblasts. INTRODUCTION: The purpose of this study was to evaluate the responses of bone to chronic glucocorticoid (GC) excess by measuring the levels of selected mRNA and microRNA (miR) in bone samples of patients with Cushing's disease (CD)...
October 4, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28977600/alk2-alk3-bmpr2-acvr2a-mediate-bmp2-induced-downregulation-of-pentraxin-3-expression-in-human-granulosa-lutein-cells
#18
Long Bai, Hsun-Ming Chang, Jung-Chien Cheng, Guiyan Chu, Peter C K Leung, Gongshe Yang
Bone morphogenetic protein 2 (BMP2) belongs to the transforming growth factor-β superfamily and plays a critical role in regulating ovarian follicle function. Currently, the role of BMP2 during cumulus expansion remains to be determined. The aim of this study was to investigate the effect of BMP2 on the regulation of pentraxin 3 (PTX3) expression (the major component of cumulus expansion) and the underlying mechanisms in human granulosa-lutein (hGL) cells. Both primary and immortalized hGL cells were used as research models...
October 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28975034/the-periosteal-requirement-and-temporal-dynamics-of-bmp2-induced-middle-phalanx-regeneration-in-the-adult-mouse
#19
Lindsay A Dawson, Ling Yu, Mingquan Yan, Luis Marrero, Paula P Schanes, Connor Dolan, Maegan Pela, Britta Petersen, Manjong Han, Ken Muneoka
Regeneration of mammalian limbs is restricted to amputation of the distal digit tip, the terminal phalanx (P3). The adjacent skeletal element, the middle phalanx (P2), has emerged as a model system to investigate regenerative failure and as a site to test approaches aimed at enhancing regeneration. We report that exogenous application of bone morphogenetic protein 2 (BMP2) stimulates the formation of a transient cartilaginous callus distal to the amputation plane that mediates the regeneration of the amputated P2 bone...
June 2017: Regeneration
https://www.readbyqxmd.com/read/28965980/remote-regulation-of-magnetic-particle-targeted-wnt-signaling-for-bone-tissue-engineering
#20
Michael Rotherham, James R Henstock, Omar Qutachi, Alicia J El Haj
Wnt signaling is critically involved in the differentiation of human Mesenchymal Stem Cells (hMSC). Wnt proteins therefore have considerable therapeutic value, but are expensive and difficult to produce. UM206 is a synthetic peptide and ligand for the Wnt receptor Frizzled. Attachment of UM206 to magnetic nanoparticles (MNP) enables the ligand-MNP complex to be manipulated using magnetic fields, allowing control of Frizzled stimulation. Using this approach, Wnt signaling was activated in hMSC which resulted in Frizzled clustering, β-catenin translocalization and activation of TCF/LEF responsive transcription...
September 29, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
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