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https://www.readbyqxmd.com/read/28648641/glypican-based-drug-releasing-titania-implants-to-regulate-bmp2-bioactivity-as-a-potential-approach-for-craniosynostosis-therapy
#1
Manpreet Bariana, Prem Dwivedi, Sarbin Ranjitkar, John A Kaidonis, Dusan Losic, Peter J Anderson
Advances in molecular biology and nanomedicine based therapies hold promise to obviate the need of multiple surgical interventions (associated with current management) in craniosynostosis by preventing bone re-ossification. One such adjunctive therapy involves application of glypicans 1 and 3 (GPC1 and GPC3) that are BMP inhibitors implicated in downregulating the BMP2 activity in prematurely fusing sutures. Electrochemically anodized Titania nanotube (TNT) arrays have been recognized as a promising localized, long-term drug delivery platform for bone-related therapies...
June 23, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28645174/adrenomedullin-promotes-the-odontogenic-differentiation-of-dental-pulp-stem-cells-through-creb-bmp2-signaling-pathway
#2
Qiang Zhu, Jianyong Gao, Gang Tian, Zhen Tang, Yinghui Tan
No abstract text is available yet for this article.
June 22, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/28643469/atra-signaling-regulates-the-expression-of-col9a1-through-bmp2-wnt4-runx1-pathway-in-antler-chondrocytes
#3
Hong-Liang Zhang, Bin Guo, Zhan-Qing Yang, Cui-Cui Duan, Shuang Geng, Kai Wang, Hai-Fan Yu, Zhan-Peng Yue
Although all-trans retinoic acid (ATRA) is involved in the regulation of cartilage growth and development, its regulatory mechanisms remain unknown. Here, we showed that ATRA could induce the expression of COL9A1 in antler chondrocytes. Silencing of cellular retinoic acid binding protein 2 (CRABP2) could impede the ATRA-induced upregulation of COL9A1, whereas overexpression of CRABP2 presented the opposite effect. RARα agonist Am80 induced the expression of COL9A1, whereas treatment with RARα antagonist Ro 41-5253 or RXRα small-interfering RNA (siRNA) caused an obvious blockage of ATRA on COL9A1...
June 22, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28643418/protein-engineering-of-recombinant-human-bone-morphogenetic-protein-2-with-higher-interaction-with-ca-phosphate-based-scaffold-usedfor-osteogenesis
#4
Mohammad Bayat, Sahar Shojaei, Naghmeh Bahrami, Abdol Reza Mohamadnia, Pegah Shojaei, Naeim Bahrami
The aim of the present studywas to assess the recombinant bonemorphogenetic protein 2 (RHBMP-2)with higher substantively and solubility for use in calcium phosphate scaffolds for better release in differentiation of mesenchymal stem cells to osteoblast cells. By using bioinformatics tools,2 mutations (p. L10D and p. S12E) werechosen and applied in BMP2 CDS sequenceto increase interaction with calcium derived composite. The new recombinant mutated sequence (BMP2(mut) ) was synthesized and then subcloned to expression vector pBV220...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28643382/synthesis-and-biological-evaluation-of-arginyl-diosgenin-conjugate-as-a-potential-bone-tissue-engineering-agent
#5
Ai-Mei Liao, Hana Jung, Ji Won Yu, Dong Hee Lee, Sang-Soo Park, Bangrong Cai, ChangJu Chun
Water-soluble arginyl-diosgenin (Arg-DG) conjugate was designed, synthesized, and evaluated for a biological activity. The Arg-DG conjugate was characterized using FT-IR, (1) H NMR, (13) C NMR, and HPLC-MS analyses, followed by a biological activity evaluation. Compared with DG, the Arg-DG conjugate showed a decreased cytotoxicity against L929 cells and an increased anti-proliferative activity against hepatocellular cells. The safety of the Arg-DG conjugate was confirmed using the highly sensitive Alamar Blue assay, which indicated that it increased the cellular metabolic activity at suitable concentrations...
June 23, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28642242/specific-expression-and-function-of-the-six3-optix-in-drosophila-serially-homologous-organs
#6
Amer Al Khatib, Natalia Siomava, Antonella Iannini, Nico Posnien, Fernando Casares
Organ size and pattern results from the integration of two positional information systems. One global, encoded by the Hox genes, links organ type with position along the main body axis. Within specific organs, local information is conveyed by signaling molecules that regulate organ growth and pattern. The mesothoracic (T2) wing and the metathoracic (T3) haltere of Drosophila represent a paradigmatic example of this coordination. The Hox gene Ultrabithorax (Ubx), expressed in the developing T3, selects haltere identity by, among other processes, modulating the production and signaling efficiency of Dpp, a BMP2-like molecule that acts as a major regulator of size and pattern...
June 22, 2017: Biology Open
https://www.readbyqxmd.com/read/28632668/low-intensity-pulsed-ultrasound-lipus-promotes-bmp9-induced-osteogenesis-and-suppresses-inflammatory-responses-in-human-periodontal-ligament-derived-stem-cells
#7
Joji Kusuyama, Toshiaki Nakamura, Tomokazu Ohnishi, Nahoko Eiraku, Kazuyuki Noguchi, Tetsuya Matsuguchi
OBJECTIVE: We previously reported that low intensity pulsed ultrasound (LIPUS) promotes marrow stromal cell (MSC) osteogenesis and suppresses the LPS-induced inflammatory response in osteoblasts. Here, we examined the effects of LIPUS on human periodontal ligament-derived stem cells (hPDLSCs) in chronic inflammatory bone disease, such as periodontitis. MATERIALS AND METHODS: hPDLSCs were collected from 3 healthy third molars. hPDLSCs were induced to differentiate by either recombinant BMP2 or BMP9 with or without daily LIPUS treatment (20 min/d)...
July 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/28624368/conservation-of-bmp2-4-expression-patterns-within-the-clade-branchiostoma-amphioxus-resolving-interspecific-discrepancies
#8
Luok Wen Yong, Stéphanie Bertrand, Jr-Kai Yu, Hector Escriva, Nicholas D Holland
In 2016, Kaji et al. concluded that the amphioxus mouth has the quality of a coelomoduct and is, therefore, not homologous to the oral opening of any other animal. They studied a Japanese population of Branchiostoma japonicum and based their conclusion, in part, on the larval expression of BMP2/4 in cells that reportedly joined the rim of the forming mouth. They did not detect transcription of that gene in any other tissues in the anterior region of the larva. Their results were almost the inverse of findings for B...
June 15, 2017: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/28615715/rnaseq-based-transcriptomics-study-of-smcs-from-carotid-atherosclerotic-plaque-bmp2-and-ids-proteins-are-crucial-regulators-of-plaque-stability
#9
Iraide Alloza, Haize Goikuria, Juan Luis Idro, Juan Carlos Triviño, José María Fernández Velasco, Elena Elizagaray, María García-Barcina, Genoveva Montoya-Murillo, Esther Sarasola, Reyes Vega Manrique, Maria Del Mar Freijo, Koen Vandenbroeck
Carotid artery atherosclerosis is a risk factor to develop cerebrovascular disease. Atheroma plaque can become instable and provoke a cerebrovascular event or else remain stable as asymptomatic type. The exact mechanism involved in plaque destabilization is not known but includes among other events smooth muscle cell (SMC) differentiation. The goal of this study was to perform thorough analysis of gene expression differences in SMCs isolated from carotid symptomatic versus asymptomatic plaques. Comparative transcriptomics analysis of SMCs based on RNAseq technology identified 67 significant differentially expressed genes and 143 significant differentially expressed isoforms in symptomatic SMCs compared with asymptomatic...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28610652/novel-insights-into-the-role-of-cell-free-seminal-mrnas-on-semen-quality-and-cryotolerance-of-spermatozoa-in-bulls-bos-taurus
#10
Munivenkatappa Shilpa, Sellappan Selvaraju, Venkataswamy GirishKumar, Sivashanmugam Parthipan, Krishnan B Binsila, Arunachalam Arangasamy, Janivara Parameshwaraiah Ravindra
The aim of the present study was to ascertain the effectiveness of seminal plasma mRNAs as markers to assess the reproductive performance of bulls. Semen samples (33 ejaculates) from 11 bulls were evaluated for sperm kinematic and functional parameters. Total RNA was isolated from cell-free seminal (cfs) using TRIzol LS reagent and the concentration of cfs-RNA was 24.4±2.3µgmL-1 seminal plasma. The cfs-RNA was fragmented to a size of 25-500bp. Of the cfs-mRNAs screened using real time PCR, expression of protamine 1 (PRM1) was positively (P<0...
June 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28602122/transcriptome-wide-analyses-of-human-neonatal-articular-cartilage-and-human-mesenchymal-stem-cells-hmscs-derived-cartilage-provide-a-new-molecular-target-for-evaluating-engineered-cartilage
#11
Rodrigo Alfonso Somoza, Diego Correa, Ivan Labat, Hal Sternberg, Megan E Forrest, Ahmad M Khalil, Michael D West, Paul J Tesar, Arnold I Caplan
Cellular differentiation comprises a progressive, multistep program that drives cells to fabricate a tissue with specific and site distinctive structural and functional properties. Cartilage constitutes one of the potential differentiation lineages that Mesenchymal Stem Cells (MSCs) can follow under the guidance of specific bioactive agents. Single agents such as TGF-β and BMP2 in unchanging culture conditions have been historically used to induce in vitro chondrogenic differentiation of MSCs. Despite the expression of traditional chondrogenic biomarkers such as type II collagen and aggrecan, the resulting tissue represents a transient cartilage rather than an in vivo articular cartilage (AC), differing significantly in structure, chemical composition, cellular phenotypes, and mechanical properties...
June 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28601154/spermatozoal-transcripts-expression-levels-are-predictive-of-semen-quality-and-conception-rate-in-bulls-bos-taurus
#12
Sivashanmugam Parthipan, Sellappan Selvaraju, Lakshminarayana Somashekar, Arunachalam Arangasamy, Muniandy Sivaram, Janivara Parameswaraiah Ravindra
Spermatozoal transcripts expression levels could be used to assess fertility potential of a male. The objective of the present study was to elucidate the predictive ability of the expression levels of growth, apoptosis and homeostasis regulating transcripts on sperm functions and fertility. The expression levels of spermatozoal RNA isolated from the neat semen samples were related to the good (discarded ejaculate, <25%; n = 7) and poor (discarded ejaculate, >40%, n = 6) quality semen producer and bulls (n = 12) with known conception rate...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28599080/bmp-signaling-governs-biliary-driven-liver-regeneration-in-zebrafish-via-tbx2b-and-id2a
#13
Tae-Young Choi, Mehwish Khaliq, Shinya Tsurusaki, Nikolay Ninov, Didier Y R Stainier, Minoru Tanaka, Donghun Shin
Upon mild liver injury, new hepatocytes originate from pre-existing hepatocytes. However, if hepatocyte proliferation is impaired, a manifestation of severe liver injury, biliary epithelial cells (BECs) contribute to new hepatocytes through BEC dedifferentiation into liver progenitor cells (LPCs), also termed oval cells or hepatoblast-like cells (HB-LCs), and subsequent differentiation into hepatocytes. Despite the identification of several factors regulating BEC dedifferentiation and activation, little is known about factors involved in the regulation of LPC differentiation into hepatocytes during liver regeneration...
June 9, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28595186/age-related-insulin-like-growth-factor-binding-protein-4-overexpression-inhibits-osteogenic-differentiation-of-rat-mesenchymal-stem-cells
#14
Jinhui Wu, Chao Wang, Xiong Miao, Yungang Wu, Jiabin Yuan, Muchen Ding, Jingfeng Li, Zhicai Shi
BACKGROUND/AIMS: Insulin-like growth factor binding proteins (IGFBP) play important roles in bone metabolism. IGFBP4 is involved in senescent-associated phenomena in mesenchymal stem cells (MSCs). The goal of the present study was to determine whether age-related IGFBP4 overexpression is associated with the impaired osteogenic differentiation potential of aged bone marrow derived MSCs. METHODS: MSCs were isolated from Sprague-Dawley rats aged 3-26 months. The bone morphogenetic protein (BMP)-2-induced osteogenic differentiation of rat MSCs was assessed by analyzing the expression levels of osteoblast marker genes [runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OC)], ALP activity and calcification...
June 9, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28588028/the-lysine-methyltransferase-smyd2-methylates-the-kinase-domain-of-type-ii-receptor-bmpr2-and-stimulates-bone-morphogenetic-protein-signaling
#15
Shuman Gao, Zhiqiang Wang, Wencai Wang, Xueli Hu, Peilin Chen, Jiwen Li, Xin Hua Feng, Jiemin Wong, James X Du
Lysine methylation of chromosomal and nuclear proteins is a well-known mechanism of epigenetic regulation, but relatively little is known about the role of this protein modification in signal transduction. Using an RNAi-based functional screening of the SMYD family of lysine methyltransferases (KMTs), we identified SMYD2 as a KMT essential for robust bone morphogenic protein (BMP)- but not TGFβ-induced target gene expression in HaCaT keratinocyte cells. A role for SMYD2 in BMP-induced gene expression was confirmed by shRNA knockdown and CRISPR/Cas9-mediated knockout of SMYD2...
June 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28586151/a-heterozygous-microdeletion-of-20p12-2-3-encompassing-prokr2-and-bmp2-in-a-patient-with-congenital-hypopituitarism-and-growth-hormone-deficiency
#16
Samuel J H Parsons, Neville B Wright, Emma Burkitt-Wright, Mars S Skae, Phillip G Murray
Congenital growth hormone deficiency is a rare disorder with an incidence of approximately 1 in 4,000 live births. Pituitary development is under the control of a multitude of spatiotemporally regulated signaling molecules and transcription factors. Mutations in the genes encoding these molecules can result in hypopituitarism but for the majority of children with congenital hypopituitarism, the aetiology of their disease remains unknown. The proband is a 5-year-old girl who presented with neonatal hypoglycaemia and prolonged jaundice...
June 6, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28584091/dissecting-bmp-signaling-input-into-the-gene-regulatory-networks-driving-specification-of-the-blood-stem-cell-lineage
#17
Arif Kirmizitas, Stuart Meiklejohn, Aldo Ciau-Uitz, Rachel Stephenson, Roger Patient
Hematopoietic stem cells (HSCs) that sustain lifelong blood production are created during embryogenesis. They emerge from a specialized endothelial population, termed hemogenic endothelium (HE), located in the ventral wall of the dorsal aorta (DA). In Xenopus, we have been studying the gene regulatory networks (GRNs) required for the formation of HSCs, and critically found that the hemogenic potential is defined at an earlier time point when precursors to the DA express hematopoietic as well as endothelial genes, in the definitive hemangioblasts (DHs)...
June 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28583517/association-studies-of-bone-morphogenetic-protein-2-gene-polymorphisms-with-acute-rejection-in-kidney-transplantation-recipients
#18
M-S Park, S K Kim, H J Park, H Seok, S W Kang, S H Lee, Y G Kim, J Y Moon, T H Kim, Y H Kim, S W Kang, J-H Chung, K H Jeong
OBJECTIVE: Bone morphogenetic proteins (BMP) belong to the transforming growth factor beta superfamily of proteins. This study was performed to evaluate the association of BMP gene polymorphisms with acute renal allograft rejection (AR) and graft dysfunction (GD) in Koreans. METHODS: Three hundred thirty-one patients who had kidney transplantation procedures were recruited. Transplantation outcomes were determined in terms of AR and GD criteria. We selected six single nucleotide polymorphisms (SNPs): rs1979855 (5' near gene), rs1049007 (Ser87Ser), rs235767 (intron), rs1005464 (intron), rs235768 (Arg190Ser), and rs3178250 (3; untranslated region)...
June 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28578012/smad1-5-mediates-bone-morphogenetic-protein-2-induced-up-regulation-of-bambi-expression-in-human-granulosa-lutein-cells
#19
Long Bai, Hsun-Ming Chang, Jung-Chien Cheng, Christian Klausen, Guiyan Chu, Peter C K Leung, Gongshe Yang
Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) is a transforming growth factor β (TGF-β) type I receptor antagonist that negatively regulates TGF-β and bone morphogenetic protein (BMP) signaling. BAMBI has been shown to be regulated by TGF-β signaling; however, whether BAMBI can be regulated by BMP signaling remains to be determined. The aim of this study was to investigate the effect of BMP2 on the regulation of BAMBI expression in human granulosa-lutein cells and the underlying mechanisms...
May 31, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28575129/the-involvement-and-possible-mechanism-of-pro-inflammatory-tumor-necrosis-factor-alpha-tnf-%C3%AE-in-thoracic-ossification-of-the-ligamentum-flavum
#20
Chi Zhang, Zhongqiang Chen, Xiangyu Meng, Mengtao Li, Li Zhang, Ann Huang
Thoracic ossification of the ligamentum flavum (TOLF) is characterized by ectopic bone formation in the ligamentum flavum and is considered to be a leading cause of thoracic spinal canal stenosis and myelopathy. However, the underlying etiology is not well understood. An iTRAQ proteomics was used to reveal the involvement of inflammation factors in TOLF. TNF-α is a pro-inflammatory cytokine implicated in the pathogenesis of many human diseases. Protein profiling analysis showed that the protein level of TNF-α increased in the ossified ligamentum flavum of TOLF, which was confirmed by western blot...
2017: PloS One
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