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https://www.readbyqxmd.com/read/28417259/comparison-between-magnetic-bead-and-qpcr-library-normalisation-methods-for-forensic-mps-genotyping
#1
Bhavik Mehta, Samantha Venables, Paul Roffey
Massively parallel sequencing (MPS) is fast approaching operational use in forensic science, with the capability to analyse hundreds of DNA identity and DNA intelligence markers in multiple samples simultaneously. The ForenSeq™ DNA Signature Kit on MiSeq FGx™ (Illumina) workflow can provide profiles for autosomal short tandem repeats (STRs), X chromosome and Y chromosome STRs, identity single nucleotide polymorphisms (SNPs), biogeographical ancestry SNPs and phenotype (eye and hair colour) SNPs from a sample...
April 18, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28391756/problems-in-mitochondrial-dna-forensics-while-interpreting-length-heteroplasmy-conundrum-of-various-sindhi-and-baluchi-ethnic-groups-of-pakistan
#2
Shahzad Bhatti, Muhammad Aslam Khan, Sana Abbas, Marcella Attimonelli, Gerardo Rodriguez Gonzalez, Hikmet Hakan Aydin, Erica Martinha Silva de Souza
The insight heterodox genetics of mtDNA infer new perspectives at the level of human mitochondrial control region heteroplasmy, which is substantial in evolutionary as well as forensic interpretation. The main goal of this study is to interrogate the recurrence and resolve the ambiguity of blurry spectrum of heteroplasmy in the human mtDNA control region of 50 Baluchi and 116 Sindhi unrelated individuals. Sanger sequencing was employed classically, that was further investigated by minisequencing. Only 20% Baluchi and 25...
April 9, 2017: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/28384978/applicability-of-demirjian-s-method-and-modified-cameriere-s-methods-for-dental-age-assessment-in-children
#3
K Pratyusha, Madu Ghanashyam Prasad, Ambati Naga Radhakrishna, Kaniti Saujanya, Narra Venkata Krishna Raviteja, Shalini Chandrasekhar
INTRODUCTION: Tooth development is extensively used for age estimation as a part of forensic science. AIM: The purpose of this study was to evaluate the reliability of Demirjian's, Cameriere's and modified Cameriere's population-specific regression equation methods in the dental age estimation of West Godavari children. MATERIALS AND METHODS: The study included orthopantomograms (OPG) of 60 children of West Godavari district with age group between 9 to 14 years on whom all the three methods were used for age estimation...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28383000/label-the-limits-of-forensic-science
#4
Robin Mejia
No abstract text is available yet for this article.
April 4, 2017: Nature
https://www.readbyqxmd.com/read/28374614/evaluation-of-optical-depths-and-self-absorption-of-strontium-and-aluminum-emission-lines-in-laser-induced-breakdown-spectroscopy-libs
#5
Bader A Alfarraj, Chet R Bhatt, Fang Yu Yueh, Jagdish P Singh
Laser-induced breakdown spectroscopy (LIBS) is a widely used laser spectroscopic technique in various fields, such as material science, forensic science, biological science, and the chemical and pharmaceutical industries. In most LIBS work, the analysis is performed using radiative transitions from atomic emissions. In this study, the plasma temperature and the product [Formula: see text] (the number density N and the absorption path length [Formula: see text]) were determined to evaluate the optical depths and the self-absorption of Sr and Al lines...
April 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28366290/microbiome-tools-for-forensic-science
#6
REVIEW
Jessica L Metcalf, Zhenjiang Z Xu, Amina Bouslimani, Pieter Dorrestein, David O Carter, Rob Knight
Microbes are present at every crime scene and have been used as physical evidence for over a century. Advances in DNA sequencing and computational approaches have led to recent breakthroughs in the use of microbiome approaches for forensic science, particularly in the areas of estimating postmortem intervals (PMIs), locating clandestine graves, and obtaining soil and skin trace evidence. Low-cost, high-throughput technologies allow us to accumulate molecular data quickly and to apply sophisticated machine-learning algorithms, building generalizable predictive models that will be useful in the criminal justice system...
March 30, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28351915/comparison-of-standard-culture-based-method-to-culture-independent-method-for-evaluation-of-hygiene-effects-on-the-hand-microbiome
#7
C Zapka, J Leff, J Henley, J Tittl, E De Nardo, M Butler, R Griggs, N Fierer, S Edmonds-Wilson
Hands play a critical role in the transmission of microbiota on one's own body, between individuals, and on environmental surfaces. Effectively measuring the composition of the hand microbiome is important to hand hygiene science, which has implications for human health. Hand hygiene products are evaluated using standard culture-based methods, but standard test methods for culture-independent microbiome characterization are lacking. We sampled the hands of 50 participants using swab-based and glove-based methods prior to and following four hand hygiene treatments (using a nonantimicrobial hand wash, alcohol-based hand sanitizer [ABHS], a 70% ethanol solution, or tap water)...
March 28, 2017: MBio
https://www.readbyqxmd.com/read/28350333/molecular-imprinting-applications-in-forensic-science
#8
REVIEW
Erkut Yılmaz, Bora Garipcan, Hirak K Patra, Lokman Uzun
Producing molecular imprinting-based materials has received increasing attention due to recognition selectivity, stability, cast effectiveness, and ease of production in various forms for a wide range of applications. The molecular imprinting technique has a variety of applications in the areas of the food industry, environmental monitoring, and medicine for diverse purposes like sample pretreatment, sensing, and separation/purification. A versatile usage, stability and recognition capabilities also make them perfect candidates for use in forensic sciences...
March 28, 2017: Sensors
https://www.readbyqxmd.com/read/28343098/developmental-validation-of-qiagen-investigator-%C3%A2-24plex-qs-kit-and-investigator-%C3%A2-24plex-go-kit-two-6-dye-multiplex-assays-for-the-extended-codis-core-loci
#9
Melanie Kraemer, Anke Prochnow, Michael Bussmann, Mario Scherer, Ralf Peist, Carolyn Steffen
The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group determined the expansion of the CODIS core loci to 20 STR plus three additional "highly recommended" loci (SE33, DY391, Amelogenin) Hares, 2015, 2012 [1,2]. The QIAGEN Investigator 24plex QS and Investigator 24plex GO! Kits are 6-dye multiplex assays that contain all markers of the expanded 23 CODIS core loci along with a unique internal performance control that is co-amplified with the STR markers...
March 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28343097/strait-razor-v2s-advancing-sequence-based-str-allele-reporting-and-beyond-to-other-marker-systems
#10
Jonathan L King, Frank R Wendt, Jie Sun, Bruce Budowle
STRait Razor has provided the forensic community a free-to-use, open-source tool for short tandem repeat (STR) analysis of massively parallel sequencing (MPS) data. STRait Razor v2s (SRv2s) allows users to capture physically phased haplotypes within the full amplicon of both commercial (ForenSeq) and "early access" panels (PowerSeq, Mixture ID). STRait Razor v2s may be run in batch mode to facilitate population-level analysis and is supported by all Unix distributions (including MAC OS). Data are reported in tables in string (haplotype), length-based (e...
March 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28338605/comparing-species-composition-of-passive-trapping-of-adult-flies-with-larval-collections-from-the-body-during-scene-based-medicolegal-death-investigations
#11
Michelle R Sanford
Collection of insects at the scene is one of the most important aspects of forensic entomology and proper collection is one of the biggest challenges for any investigator. Adult flies are highly mobile and ubiquitous at scenes, yet their link to the body and the time of colonization (TOC) and post-mortem interval (PMI) estimates is not well established. Collection of adults is widely recommended for casework but has yet to be rigorously evaluated during medicolegal death investigations for its value to the investigation...
March 24, 2017: Insects
https://www.readbyqxmd.com/read/28337845/nurses-attitudes-towards-the-use-of-prn-psychotropic-medications-in-acute-and-forensic-mental-health-settings
#12
Lesley Barr, Dianne Wynaden, Karen Heslop
Many countries now have national mental health policies and guidelines to decrease or eliminate the use of seclusion and restraint yet the use of Pro Re Nata (PRN) medications has received less practice evaluation. This research aimed to identify mental health nurses' attitudes towards the use of PRN medications with mental health consumers. Participants were working in forensic mental health and non-forensic acute mental health settings. The "Attitudes towards PRN medication use survey" was used and data were collected online...
March 24, 2017: International Journal of Mental Health Nursing
https://www.readbyqxmd.com/read/28291039/performance-validity-testing-in-neuropsychology-scientific-basis-and-clinical-application-a-brief-review
#13
Michael R Greher, Thomas R Wodushek
Performance validity testing refers to neuropsychologists' methodology for determining whether neuropsychological test performances completed in the course of an evaluation are valid (ie, the results of true neurocognitive function) or invalid (ie, overly impacted by the patient's effort/engagement in testing). This determination relies upon the use of either standalone tests designed for this sole purpose, or specific scores/indicators embedded within traditional neuropsychological measures that have demonstrated this utility...
March 2017: Journal of Psychiatric Practice
https://www.readbyqxmd.com/read/28284444/investigating-tnt-loss-between-sample-collection-and-analysis
#14
Niamh Nic Daeid, Holly A Yu, Matthew S Beardah
Explosives residues are often collected from explosion scenes, and from surfaces suspected of being in contact with explosives, by swabbing with solvent-wetted cotton swabs. It is vital that any explosives traces present on the swabs are successfully extracted and detected when received in a laboratory. However, a 2007 proficiency test initiated by the European Network of Forensic Science Institutes (ENFSI) Expert Working Group on Explosives involving TNT-spiked cotton swabs highlighted that explosives may not always be detected from such samples...
March 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28284440/thinking-forensics-cognitive-science-for-forensic-practitioners
#15
REVIEW
Gary Edmond, Alice Towler, Bethany Growns, Gianni Ribeiro, Bryan Found, David White, Kaye Ballantyne, Rachel A Searston, Matthew B Thompson, Jason M Tangen, Richard I Kemp, Kristy Martire
Human factors and their implications for forensic science have attracted increasing levels of interest across criminal justice communities in recent years. Initial interest centred on cognitive biases, but has since expanded such that knowledge from psychology and cognitive science is slowly infiltrating forensic practices more broadly. This article highlights a series of important findings and insights of relevance to forensic practitioners. These include research on human perception, memory, context information, expertise, decision-making, communication, experience, verification, confidence, and feedback...
March 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28283877/differences-in-local-and-national-database-recordings-of-deaths-from-suicide
#16
Amy E Austin, Corinna van den Heuvel, Roger W Byard
A search was undertaken at Forensic Science South Australia (FSSA) for all cases of suicide registered in South Australia, Australia, over a 10-year period from January 2003 to December 2012. More recent data was not accessioned as not all contemporary cases may have been completed or formally registered. The data were compared to corresponding numbers on two national registers: the National Coronial Information System (NCIS) and the Australian Bureau of Statistics (ABS). A total of 2105 cases of suicide were registered at FSSA, with a death rate of 13...
March 11, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28279856/a-time-period-study-on-the-efficiency-of-luminol-in-the-detection-of-bloodstains-concealed-by-paint-on-different-surfaces
#17
Deepthi Nagesh, Shayani Ghosh
Forensic Science is the application of science to the criminal and civil laws that are enforced by police agencies in a criminal justice system. It is a science which relies on physical evidence; one of the important physical evidences being blood. The purpose of this research was to determine the efficacy of luminol reagent in detecting bloodstains on different surfaces, concealed by multiple layers of paint, over a period of time and also to compare the intensities of chemiluminescence exhibited by them. In this study, dry wall, wooden planks and metal surfaces were identified as commonly encountered surfaces at crime scenes and hence 25 of each surface were simulated and blood was spattered, which were then concealed by progressive layers of paint specific to each surface...
February 11, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28271501/preparation-of-artificial-blood-from-the-extract-of-legume-root-nodules-and-the-creation-of-artificial-latent-fingermarks-in-blood-using-artificial-blood
#18
Sungwook Hong, Chaewon Kim, Soyoung Jeon, Eunhye Lee
Distribution of homogeneous fingermarks in blood is essential for conducting proficiency tests in forensic science. Hence, the artificial blood was prepared using the root nodule extract of Glycine max plants. The reactivity of the artificial blood with widely used human blood detection reagents was tested. Artificial latent fingermarks in blood were printed using an inkjet cartridge case filled with artificial blood solution. The artificial latent fingermarks in blood were developed with amino acid-sensitive reagents and could obtain development as prominent as the image of the master fingermark saved on the computer...
March 7, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28270824/advances-of-community-level-plant-dna-barcoding-in-china
#19
Nancai Pei, Bufeng Chen, W J Kress
DNA barcoding is a commonly used bio-technology in multiple disciplines including biology, environmental science, forensics and inspection, etc. Forest dynamic plots provide a unique opportunity to carry out large-scale, comparative, and multidisciplinary research for plant DNA barcoding. The paper concisely reviewed four previous progresses in China; specifically, species discrimination, community phylogenetic reconstruction, phylogenetic community structure exploration, and biodiversity index evaluation. Further, we demonstrated three major challenges; specifically, building the impetus to generate DNA barcodes using multiple plant DNA markers for all woody species at forest community levels, analyzing massive DNA barcoding sequence data, and promoting theoretical innovation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28270460/a-positive-behavioral-approach-for-aggression-in-forensic-psychiatric-settings
#20
Peter Tolisano, Tracey M Sondik, Charles C Dike
Aggression toward self and others by complex patients admitted to forensic psychiatric settings is a relatively common yet extremely difficult behavior to treat. Traditional interventions in forensic inpatient settings have historically emphasized control and management over treatment. Research over the past several years has demonstrated the value of behavioral and psychosocial treatment interventions to reduce aggression and to increase prosocial skill development in inpatient forensic population. Positive behavioral support (PBS) offers a comprehensive approach that incorporates the science of applied behavioral analysis (ABA) in support of patients with challenging behaviors, including aggression and violence...
March 2017: Journal of the American Academy of Psychiatry and the Law
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