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https://www.readbyqxmd.com/read/29166640/lactic-acid-bacteria-from-andean-grain-amaranth-a-source-of-vitamins-and-functional-value-enzymes
#1
Silvana L Carrizo, Cecilia E Montes de Oca, María Elvira Hébert, Lucila Saavedra, Graciela Vignolo, Jean Guy LeBlanc, Graciela Celestina Rollán
Amaranth is a rediscovered pseudocereal with high nutritional properties. Lactic acid fermentation can increase the functional and nutritional value of foods. The aims of this study were to isolate and evaluate the functionality of lactic acid bacteria (LAB) from amaranth. LAB strains (n = 29) isolated from amaranth sourdough and grains included Lactobacillus (L.) plantarum (n = 8), L. rhamnosus (n = 6), Enterococcus (E.) mundtii (n = 4), E. hermanniensis (n = 3), E. durans (n = 1), Enterococcus sp. (n = 1), Leuconostoc (Lc...
November 23, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29166048/symmetry-breaking-and-spectral-considerations-of-the-surprisingly-floppy-c-c3h-radical-and-the-related-dipole-bound-excited-state-of-c-c3h
#2
Matthew K Bassett, Ryan C Fortenberry
The C3H radical is believed to be prevalent throughout the interstellar medium and may be involved in the formation of polycyclic aromatic hydrocarbons. C3H exists as both a linear and a cyclic isomer. The C2v cyclopropenylidenyl radical isomer was detected in the dark molecular cloud TMC-1, and the linear propenylidenyl radical isomer has been observed in various dark molecular clouds. Even though the c-C3H radical has been classified rotationally, the vibrational frequencies of this seemingly important interstellar molecule have never been directly observed...
June 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29163817/characteristics-and-genetic-diversity-of-multi-drug-resistant-extended-spectrum-beta-lactamase-esbl-producing-escherichia-coli-isolated-from-bovine-mastitis
#3
Tariq Ali, Sadeeq Ur Rahman, Limei Zhang, Muhammad Shahid, Dandan Han, Jian Gao, Shiyao Zhang, Pamela L Ruegg, Umer Saddique, Bo Han
A characterization of the drug resistance profiles, identification of PCR-based replicon typing, and multilocus sequence typing (MLST) and analysis of 46 ESBL-producing Escherichia coli from cows with mastitis are described. All multidrug-resistant isolates of various phylogenetic groups (A = 31, B1= 3, B2 = 2, D = 10) were ESBL-producers of genotypes CTX-M-15 (29), CTX-M-55 (4), CTX-M-14 (4), CTX-M-3 (1), CTX-M-1 (1), TEM (22) and SHV (8) that were found on conjugative plasmids of diverse incompatibility groups (primarily IncF)...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163508/interferon-%C3%AE-enhanced-cd100-plexin-b1-b2-interactions-promote-natural-killer-cell-functions-in-patients-with-chronic-hepatitis-c-virus-infection
#4
Yu He, Yonghong Guo, Chao Fan, Yingfeng Lei, Yun Zhou, Mingjie Zhang, Chuantao Ye, Guangxi Ji, Li Ma, Jianqi Lian, Jonathan P Moorman, Zhi Q Yao, Jiuping Wang, Chunqiu Hao, Ying Zhang, Zhansheng Jia
Background: CD100, also known as Sema4D, is an immune semaphorin constitutively expressed on natural killer (NK) cells and T cells. As an immune activation molecule, CD100 has important immunoregulatory effects on NK functions by enhancing the interactions between NK cells and target cells. The aim of this study was to investigate whether hepatitis C virus (HCV) infection affects CD100 expression, and whether interferon-α treatment enhances NK killing activity to facilitate HCV clearance via CD100...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29160182/enhanced-bone-regeneration-via-diabetic-cell-based-adenoviral-bmp2-gene-therapy-in-diabetic-animals
#5
Shin-Young Park, Kyoung-Hwa Kim, Chan Ho Park, Seung-Yun Shin, In-Chul Rhuy, Yong-Moo Lee, Yang Jo Seol
The application of bone morphogenetic protein 2 (BMP-2) has been extensively investigated to improve diabetes-impaired bone healing; however, the delivery of BMP-2 via gene therapy for bone regeneration has rarely been investigated in diabetic animals. In this study, we aimed to evaluate which cells induce more new bone formation in diabetic animals when cell-based BMP2 gene therapy is applied. For this purpose, we harvested bone marrow stromal cells (BMSCs) twice in the same animal before (non-diabetic BMSCs; nBMSCs) and after diabetes induction (diabetic BMSCs; dBMSCs) using modified bone marrow ablation methods...
November 21, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29157467/broad-spectrum-immunoaffinity-cleanup-for-the-determination-of-aflatoxins-b1-b2-g1-g2-m1-m2-in-ophiocordyceps-sinensis-and-its-pharmaceutical-preparations-by-ultra-performance-liquid-chromatography-tandem-mass-spectrometry
#6
Shujuan Sun, Jie Xie, Tao Peng, Bing Shao, Kui Zhu, Yuanze Sun, Kai Yao, Qiang Gu, Jing Zhang, Chunlin Fan, Ying Chen, Haiyang Jiang
An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 (AFB1, AFB2, AFG1, AFG2, AFM1 and AFM2) in Ophiocordyceps sinensis and its pharmaceutical preparations. A rapid and reliable immunoaffinity column containing a broad-spectrum monoclonal antibody for six aflatoxins was used for sample cleanup. Under the optimized conditions, the home-made immunoaffinity column capacity were about 315, 319, 292, 102, 444 and 369ng/mL gel for AFB1, AFB2, AFG1, AFG2, AFM1 and AFM2, respectively...
November 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29156489/an-adherent-mucus-layer-attenuates-the-genotoxic-effect-of-colibactin
#7
Christian Reuter, Mona Alzheimer, Heike Walles, Tobias A Oelschlaeger
The human gastrointestinal tract is a complex ecosystem in which epithelial cells and microorganisms of the intestinal microbiota live in symbiosis. Certain members of the microbiota, in particular Escherichia coli strains of the B2 phylotype, carry the polyketide synthase (pks)-island encoding the genotoxin colibactin. Colibactin is a non-ribosomal peptide (NRP) or polyketide (PK)-NRP hybrid of still unsolved structure, which induces DNA double strand breaks (DSBs) in eukaryotic cells. However, direct contact between live bacteria and host cell is required in order to elicit these genotoxic effects...
November 20, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29156018/risk-factors-of-myasthenic-crisis-after-thymectomy-for-thymoma-patients-with-myasthenia-gravis
#8
Liang Xue, Lin Wang, Jihong Dong, Yunfeng Yuan, Hong Fan, Yi Zhang, Qun Wang, Jianyong Ding
OBJECTIVES: Total thymectomy should be performed on thymoma patients with myasthenia gravis. The aim of the present study was to investigate the risk factors of postoperative myasthenic crisis (POMC) occurrence in these patients. METHODS: The clinical records of 127 thymoma patients with myasthenia gravis (68 men, 59 women; median age, 50 years) who underwent total thymectomy at our institution from 2005 to 2014 were retrospectively reviewed. The following factors were analysed in relation to POMC: gender, age, duration of symptoms, bulbar symptoms, smoking history, history of myasthenic crisis, comorbidities, perioperative pyridostigmine and prednisolone therapy, spirometric and blood gas parameters, Osserman stage, operation approach, major complications, World Health Organization (WHO) histologic classification, Masaoka stage and use of immunoglobulins or plasmapheresis...
October 1, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29155368/urinary-11-dehydro-thromboxane-b2-levels-are-associated-with-vascular-inflammation-and-prognosis-in-atherosclerotic-cardiovascular-disease
#9
Nan Wang, Kimberly C Vendrov, Brian P Simmons, Robert N Schuck, George A Stouffer, Craig R Lee
Cyclooxygenase-derived thromboxane (TxA2) and prostacyclin (PGI2) regulate atherogenesis in preclinical models. However, the relationship between TxA2 and PGI2 biosynthesis, vascular inflammation, and atherosclerotic cardiovascular disease (ASCVD) progression in humans remains unclear. The association between stable urine metabolites of thromboxane (TxA2-M) and prostacyclin (PGI2-M), circulating levels of cellular adhesion molecules (CAMs: E-selectin, P-selectin), chemokines and C-reactive protein, and the incidence of major adverse cardiovascular events (MACE) were evaluated in 120 patients with stable ASCVD on aspirin therapy...
November 16, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/29154716/occurrence-of-toxigenic-fungi-and-mycotoxins-during-smoked-paprika-production
#10
Rocío Casquete, Alicia Rodríguez, Alejandro Hernández, Alberto Martín, Teresa Bartolomé, Juan José Córdoba, María G Córdoba
'La Vera' smoked paprika is a traditional Spanish product regulated under a protected designation of origin. Mycotoxins are possible contaminants in paprika, yet there is little information about mycotoxin production during the processing of smoked paprika. In this study, samples of dried peppers collected from six traditional dryers from four producers were evaluated for physicochemical parameters, mycotoxins, and mycotoxin-producing fungi. The moisture content and water activity of the peppers ranged from 11...
November 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29152006/anatomic-shoulder-arthroplasty-technical-considerations
#11
REVIEW
Bogdan A Matache, P Lapner
Osteoarthritis of the shoulder is a common condition in the aging population, and it can have profound effects on patients' quality of life. The anatomic total shoulder arthroplasty is a well-described treatment modality resulting generally excellent outcomes. The objective of this review is to discuss the technical aspects of primary anatomic total shoulder arthroplasty, and to provide a framework to follow to achieve a successful surgical result. The topics covered include preoperative planning, surgical considerations, and approaches, humeral preparation, glenoid bone loss and the emerging concept of using the reverse total shoulder arthroplasty for the type B2 glenoid...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/29149242/long-term-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebsiella-pneumoniae-in-the-general-population-in-the-netherlands
#12
Engeline van Duijkeren, Cornelia C H Wielders, Cindy M Dierikx, Angela H A M van Hoek, Paul Hengeveld, Christiaan Veenman, Alice Florijn, Aniek Lotterman, Lidwien A M Smit, Jaap T van Dissel, Catharina B M Maassen, Sabine C de Greeff
Background: This longitudinal study aimed to investigate (risk factors for) persistence of carriage and molecular characteristics of extended-spectrum and pAmpC β-lactamase-producing (ESBL/pAmpC) Escherichia coli and Klebsiella pneumoniae (ESBL-E/K) in adults in the Dutch community. Methods: Following a cross-sectional study (ESBL-E/K prevalence 4.5%), a subset of ESBL-E/K positive (n=76) and negative (n=249) individuals volunteered to provide five monthly faecal samples and questionnaires...
November 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29146942/efficient-near-infrared-light-emitting-electrochemical-cell-nir-leec-based-on-new-binuclear-ruthenium-phenanthroimidazole-exhibiting-desired-charge-carrier-dynamics
#13
Babak Nemati Bideh, Hashem Shahroosvand
Near-infrared light-emitting electrochemical cell (NIR-LEEC) has emerged as a new and promising lighting sourcewhich could serve as low-cost alternatives in NIR light-emitting sources which are typically expensive. LECs were also shown advantages such as light weight, simplicity and low operation voltages. However, only a few examples of NIR-LEEC are reported in which external quantum efficiency(EQE) of devices limited to 0.1%. Here, we report, efficient NIR-LEEC based of two novel binuclear ruthenium phenanthroimidzole complex which differ by employing the type of ancillary ligand including 2, 2'bipyridine (bpy) (B1) and 4, 4' dimethyl bpy (B2) that realize maximum EQE of 0...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146616/therapeutic-potential-of-afatinib-for-cancers-with-erbb2-her2-transmembrane-domain-mutations-g660d-and-v659e
#14
Hiromasa Yamamoto, Shinichi Toyooka, Takashi Ninomiya, Shigemi Matsumoto, Masashi Kanai, Shuta Tomida, Katsuyuki Kiura, Manabu Muto, Ken Suzawa, Patrice Desmeules, Mark G Kris, Bob T Li, Marc Ladanyi
We previously reported on a family with hereditary lung cancer, in which a germline mutation in the transmembrane domain (G660D) of avian erythroblastic leukemia viral oncogene homolog 2 (erb-b2 receptor tyrosine kinase 2) (ERBB2; human epidermal growth factor receptor 2 [HER2]) seemed to be responsible for the cancer predisposition. Although few data are available on treatment, anti-ERBB2 therapeutic agents may be effective for ERBB2-mutant cancers. The familial lung cancer patient in one of the authors' institutes developed bone metastasis with enlarging lung tumors and was treated with the ERBB2 inhibitor afatinib...
November 16, 2017: Oncologist
https://www.readbyqxmd.com/read/29146325/study-of-human-salivary-proline-rich-proteins-interaction-with-food-tannins
#15
Susana Soares, Ignacio García-Estévez, Raúl Ferrer-Galego, Natércia F Brás, Elsa Brandão, Mafalda Silva, Natércia Teixeira, Fátima Fonseca, Sérgio F Sousa, Frederico Ferreira-da-Silva, Nuno Mateus, Victor de Freitas
In this work, saturation transfer difference-NMR, isothermal microcalorimetry and molecular dynamics simulations have been used to study the individual interactions between basic, glycosylated and acidic proline-rich proteins (bPRPS, gPRPs, aPRPs) and P-B peptide with some representative food tannins [procyanidin B2, procyanidin B2 3'-O-gallate (B2g) and procyanidin trimer (catechin-4-8-catechin-4-8-catechin)]. Results showed that P-B peptide was in general the salivary protein (SP) with higher affinity whereas aPRPs showed lower affinity to the studied procyanidins...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29145311/pulmonary-hypertension-as-a-manifestation-of-mitochondrial-disease-a-case-report-and-review-of-the-literature
#16
Shan Xu, Xiaoling Xu, Jisong Zhang, Kejing Ying, Yuquan Shao, Ruifeng Zhang
BACKGROUND: Mitochondrial diseases are a group of multisystem heterogeneous diseases caused by pathologic dysfunction of the mitochondrial respiratory chain. A wide range of clinical expression has been described. However, pulmonary hypertension has rarely been described in association with mitochondrial disease until the past decade, and there is no currently recognized treatment for the pulmonary hypertension complicated with mitochondrial disorder. PATIENT CONCERNS: We reported the case of a 15-year-old boy who presented with shortness of breath and exercise limitation after a cold, and the diagnosis of pulmonary hypertension was confirmed by right heart catheter...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29145280/liver-stiffness-measurement-and-spleen-diameter-as-predictors-for-the-presence-of-esophageal-varices-in-chronic-hepatitis-c-patients
#17
Mohammed Tag-Adeen, Mohamed Alsenbesy, Ali Abdelrahman Ghweil, M Ali Hussein Abd Elrazek, Elsayed A Elgohary, Mohammad M Sallam, Ali Ismael, Abdallah Nawara
Although it is an invasive and unpleasant procedure, esophagogastroduodenoscopy (EGD) is still the gold standard for esophageal varices (EV) detection. The aim of this study was to investigate liver stiffness measurement (LSM) and spleen diameter as simple noninvasive tools for EV prediction in chronic hepatitis C patients (CHC).A total of 123 Egyptian patients with CHC have been included and were classified based on screening EGD result into 2 groups; group A (without EV) and group B (with EV). Group (B) was subclassified according to EV grade into 4 subgroups: (B1, grade I), (B2, grade II), (B3, grade III), and (B4, grade IV)...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29144210/a-new-allele-of-the-kappa-casein-gene-in-local-zebu-cattle-breeds
#18
Tauseef Ahmad, Ashwin Atkulwar, Sameera Farah, Vijendra Singh, Mumtaz Baig
Local zebu cattle breeds in Indian villages are crossbred with exotic breeds at an unprecedented rate without utilising the full potential of genomic technologies. In addition to agriculture produce, livestock, particularly cattle, constitute a vital source of livelihood for farmers in India. Age-old agricultural practices, errant monsoon, and frequent crop failures have resulted in Maharashtra having the highest number of farmer suicides in the country. Local cattle breeds are considered low-yield breeds and thus are primarily used as beasts of burden...
November 16, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/29143864/correction-to-cell-type-specific-localization-of-ephs-pairing-with-ephrin-b2-in-the-rat-postnatal-pituitary-gland
#19
Saishu Yoshida, Takako Kato, Naoko Kanno, Naoto Nishimura, Hiroto Nishihara, Kotaro Horiguchi, Yukio Kato
The published online version contains mistakes in Table 1, Table 2 and Fig. 2. See below for the corrected Tables and Figure.
November 16, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29142198/shock-synthesis-of-decagonal-quasicrystals
#20
J Oppenheim, C Ma, J Hu, L Bindi, P J Steinhardt, P D Asimow
The Khatyrka meteorite contains both icosahedral and decagonal quasicrystals. In our previous studies, icosahedral quasicrystals have been synthesized and recovered from shock experiments at the interface between CuAl5 and stainless steel 304 alloys. In this study, we report a new shock recovery experiment aimed at synthesizing decagonal quasicrystals similar to decagonite, natural Al71Ni24Fe5. Aluminum 2024 and permalloy 80 alloys were stacked together and shocked in a stainless steel 304 recovery chamber...
November 15, 2017: Scientific Reports
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