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https://www.readbyqxmd.com/read/29785744/fumonisin-b1-actuates-oxidative-stress-associated-colonic-damage-via-apoptosis-and-autophagy-activation-in-murine-model
#1
Sang Ho Kim, Mahendra Pal Singh, Chanchal Sharma, Sun Chul Kang
In the present study, we investigated the cytotoxic mechanism of Fumonisin B1 (FB1) in mice colonic region in a time course manner. Herein, after consecutive 4 days of exposure to FBI (2.5 mg/kg body weight), we observed disintegration of mice colon, as evidenced by histopathological analysis. FB1 significantly increased alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities in serum and plasma, decreased ceramide level, increased sphinganine level, and increased lipid peroxidase level along with the breakdown of the antioxidant system...
May 22, 2018: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/29785590/clinical-application-of-a-minimally-invasive-cement-augmentable-schanz-screw-rod-system-to-treat-pelvic-ring-fractures
#2
Paul Schmitz, Florian Baumann, Yves P Acklin, Boyko Gueorguiev, Michael Nerlich, Stephan Grechenig, Michael Bernd Müller
PURPOSE: The purpose of this study is to analyze the results using the USS fracture MIS system (DePuy Synthes) to treat instable pelvic ring fractures. As its outstanding feature, it is the only Schanz screw and rod system at present that combines angular stability, perforation/fenestration of the screws for cement-augmentation, a variable screw length, and a large screw diameter. MATERIALS AND METHODS: Retrospective investigation of 134 pelvic ring fractures treated in 2012-2013...
May 21, 2018: International Orthopaedics
https://www.readbyqxmd.com/read/29785517/optimization-and-enhancement-of-textile-reactive-remazol-black-b-decolorization-and-detoxification-by-environmentally-isolated-ph-tolerant-pseudomonas-aeruginosa-ky284155
#3
Rasha A Hashem, Reham Samir, Tamer M Essam, Amal E Ali, Magdy A Amin
Azo dyes are complex derivatives of diazene used in food and textile manufacture. They are highly recalcitrant compounds, and account for severe environmental and health problems. Different strains of Pseudomonas species were isolated from textile wastewater effluents. The bioconversion of Remazol black B (a commonly used water soluble dye) by Pseudomonas aeruginosa was observed in static conditions. The bio-decolorization process was optimized by a multi factorial Plackett-Burman experimental design. Decolorization of 200 mg L-1 reached 100% in 32 h...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29785357/prognostic-significance-of-cyclins-a2-b1-d1-and-e1-and-ccnd1-numerical-aberrations-in-oral-squamous-cell-carcinomas
#4
Luís Silva Monteiro, Márcio Diniz-Freitas, Saman Warnakulasuriya, Tomás Garcia-Caballero, Jerónimo Forteza-Vila, Máximo Fraga
We analysed the expression of cyclins A2, B1, D1, and E1 by immunohistochemistry and numerical aberrations in CCND1 gene by fluorescence in situ hybridization technique in 67 primary oral squamous cell carcinomas (OSCC). Cyclin A2 expression was observed in 54 (83.1%) tumours, cyclin D1 in 58 (89.2%), cyclin B1 in 39 (60%), and cyclin E in 21 (32.8%). CCND1 region analysis revealed 26 (43.3%) tumours with the presence of numerical aberrations which were correlated with cyclin D1 high expression (Rho = 0...
2018: Analytical Cellular Pathology (Amsterdam)
https://www.readbyqxmd.com/read/29785044/the-live-cell-dna-stain-sir-hoechst-induces-dna-damage-responses-and-impairs-cell-cycle-progression
#5
Onur Sen, Adrian T Saurin, Jonathan M G Higgins
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10-25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784851/replacement-by-site-saturation-mutagenesis-of-residue-119-in-ndm-1-metallo-%C3%AE-lactamase-a-kinetic-study
#6
Francesca Marcoccia, Paola Sandra Mercuri, Moreno Galleni, Giuseppe Celenza, Gianfranco Amicosante, Mariagrazia Perilli
NDM-1 is a subclass B1 metallo-β-lactamase which exhibits a broad activity spectrum against β-lactam antibiotics. In this study we report the kinetic study of six Q119X variants obtained by site-directed mutagenesis on NDM-1. All Q119X variants were able to hydrolyze very efficiently carbapenems, penicillins and 1st , 2nd , 3rd and 4th generation cephalosporins. In particular, Q119E, Q119Y, Q119V and Q119K mutants showed an improvement of kcat/ Km towards penicillins respect to NDM-1. The catalytic efficiency of Q119K variant is about 65- and 70-fold higher than that of NDM-1 for benzylpenicillin and carbenicillin, respectively...
May 21, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29784365/rapid-detection-of-four-mycotoxins-in-corn-using-a-microfluidics-and-microarray-based-immunoassay-system
#7
Yiqi Chen, Xiangrui Meng, Yunzeng Zhu, Minjie Shen, Ying Lu, Jing Cheng, Youchun Xu
Mycotoxins threaten human health seriously because they usually exist in food, fodder and commodities. In this study, a rapid and sensitive immunoassay system for commonly encountered mycotoxins was established based on microfluidics and protein microarrays. Four mycotoxins (T-2 toxin, aflatoxin B1, ochratoxin A, and zearalenone) can be automatically detected in a custom-made microdevice within 30 min under the assistance of a prototype of the instrument with a fluid control system and an imaging system. Once the microdevices are fabricated, they are small-sized and user-friendly...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784363/a-colorimetric-immunoassay-based-on-glucose-oxidase-induced-aunp-aggregation-for-the-detection-of-fumonisin-b-1
#8
Xirui Chen, Yi Liang, Wenjing Zhang, Yuankui Leng, Yonghua Xiong
Naked-eye readout colorimetric signal-based assays are promising for high-throughput screening detection and point-of-care diagnostics in resource-constrained regions. Here, a novel direct competitive plasmonic enzyme-linked immunosorbent assay (dc-pELISA) based on gold nanoparticle (AuNP) aggregation with highly sensitive and robust naked-eye readout signal was developed and used to detect fumonisin B1 (FB1 ). AuNP aggregation was induced by a horseradish peroxidase (HRP)/hydrogen peroxide (H2 O2 )/tyramine (TYR) system, resulting in a dramatic color change from red to blue...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29782917/detoxification-and-immunoprotection-of-zn-ii-curcumin-in-juvenile-pacific-white-shrimp-litopenaeus-vannamei-feed-with-aflatoxin-b1
#9
Ying-Ying Yu, Jin Niu, Pen Yin, Yong-Jian Liu, Li-Xia Tian, Donghui Xu
Aflatoxins, which was produced by Aspergillus flavus or Aspergillus parasiticus fungi during grain and feed processing or storage, could cause severe health problems and reduction of yield during shrimp cultures. To evaluate toxic effects of aflatoxin B1 (AFB1) in juvenile Pacific white shrimp (Litopenaeus vannamei) and potential protective effect of Zn(II)-curcumin (Zn-CM), four experimental diets (control, 500 μg/kg AFB1, 500 μg/kg AFB1+100 mg/kg Zn-CM, 500 μg/kg AFB1+200 mg/kg Zn-CM) were formulated in quadruplicate to feed the shrimp for 8 weeks...
May 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29782351/role-of-lkb1-in-migration-and-invasion-of-cr-vi-transformed-human-bronchial-epithelial-beas-2b-cells
#10
Jian Lu, Zhongping Zhou, Miaomiao Tang, Haijun Shen, Yi Liu, Jin Wang, Yewen Jiang, Yifan Chen, Zhanao Wu
Hexavalent chromium [Cr(VI)] is a common human carcinogen associated with lung cancer and other pulmonary diseases as exposure to excessive Cr(VI) induces malignant transformation in human lung epithelial cells. The mechanism underlying its carcinogenicity is unclear in terms of how it facilitates metastases. Cr(VI) compounds are reported to briefly promote cell migration in a concentration-dependent manner and oncogene liver kinase B1 (LKB1) was reduced in Cr(VI)-transformed cells. Overexpression of LKB1 in Beas-2B-Cr [Cr(VI) malignantly transformed Beas-2B cells] suppressed cell migration and invasion and inactivated FAK, Src, MMP-2, GSK3β, β-catenin, and HEF1, which contribute to cell migration and invasion...
May 18, 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29781609/enhancement-and-analysis-of-human-anti-aflatoxin-b1-afb1-scfv-antibody-ligand-interaction-using-chain-shuffling
#11
Kuntalee Rangnoi, Kiattawee Choowongkomon, Richard O'Kennedy, Florian Rüker, Montarop Yamabhai
A human anti-aflatoxin B1 (AFB1) scFv antibody (yAFB1-c3), selected from a naïve human phage-displayed scFv library, was used as a template for improving and analysis of antibody-ligand interactions using the chain-shuffling technique. The variable heavy and variable light (VH/VL) shuffled library was constructed from the VH of 25 pre-selected clones recombined with the VL of yAFB1-c3 and vice versa. Affinity selection from these library demonstrated that the VH domain played an important role in the binding of scFv to free AFB1...
May 21, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29780443/separase-inhibitor-sepin-1-inhibits-foxm1-expression-and-breast-cancer-cell-growth
#12
Nenggang Zhang, Debananda Pati
Sepin-1, a potent non-competitive inhibitor of separase, inhibits cancer cell growth, but the mechanisms of Sepin-1-mediated growth inhibition are not fully understood. Here we report that Sepin-1 hinders growth of breast cancer cells, cell migration, and wound healing. Inhibition of cell growth induced by Sepin-1 in vitro doesn't appear to be through apoptosis but rather due to growth inhibition. Following Sepin-1 treatment caspases 3 and 7 are not activated and Poly (ADP-ribose) polymerase (Parp) is not cleaved...
2018: Journal of Cancer Science & Therapy
https://www.readbyqxmd.com/read/29779877/developmental-upregulation-of-ephrin-b1-silences-sema3c-neuropilin-1-signaling-during-post-crossing-navigation-of-corpus-callosum-axons
#13
Erik Mire, Mélanie Hocine, Elsa Bazellières, Thomas Jungas, Alice Davy, Sophie Chauvet, Fanny Mann
The corpus callosum is the largest commissure in the brain, whose main function is to ensure communication between homotopic regions of the cerebral cortex. During fetal development, corpus callosum axons (CCAs) grow toward and across the brain midline and then away on the contralateral hemisphere to their targets. A particular feature of this circuit, which raises a key developmental question, is that the outgoing trajectory of post-crossing CCAs is mirror-symmetric with the incoming trajectory of pre-crossing axons...
May 10, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29779163/discovering-simple-phenylboronic-acid-and-benzoxaborole-derivatives-for-experimental-oncology-phase-cycle-specific-inducers-of-apoptosis-in-a2780-ovarian-cancer-cells
#14
Mateusz Psurski, Agnieszka Łupicka-Słowik, Agnieszka Adamczyk-Woźniak, Joanna Wietrzyk, Andrzej Sporzyński
Objective The aim of the study was to evaluate the antiproliferative potential of simple phenylboronic acid and benzoxaborole derivatives as well as to provide preliminary insight into their mode of action in cancer cells in vitro. Methods The antiproliferative activity was assessed in five diverse cancer cell lines via the SRB method (sulforhodamine B) or MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method after 72 h of treatment. Further studies of the mechanism of action consisted of the influence of the compounds on cell cycle progression and apoptosis induction, which was assessed by flow cytometry, caspase-3 enzymatic activity, fluorescence microscopy and western blot analysis...
May 19, 2018: Investigational New Drugs
https://www.readbyqxmd.com/read/29778206/occurrence-and-characterisation-of-esbl-encoding-plasmids-among-escherichia-coli-isolates-from-fresh-vegetables
#15
Christin Freitag, G B Michael, Jun Li, Kristina Kadlec, Yang Wang, Melanie Hassel, Stefan Schwarz
Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates have been increasingly reported in different reservoirs. The aims of this study were to investigate the presence of ESBL-producing E. coli in fresh vegetables and to characterise their ESBL gene-carrying plasmids. Among the 245 samples from vegetables investigated during 2011-2013, seven putative ESBL-producing E. coli (salad n = 2, sprouts n = 5) were found. They were subjected to ESBL phenotypic confirmatory tests, detection/sequencing of ESBL genes, antimicrobial susceptibility testing (AST), phylotyping, XbaI-macrorestriction analysis, multilocus sequence typing and transformation...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778183/high-genetic-diversity-of-extended-spectrum-%C3%AE-lactamases-producing-escherichia-coli-in-feces-of-horses
#16
Syndia Sadikalay, Yann Reynaud, Stéphanie Guyomard-Rabenirina, Mélanie Falord, Célia Ducat, Laetitia Fabre, Simon Le Hello, Antoine Talarmin, Séverine Ferdinand
Extended-spectrum beta-lactamases (ESBLs), especially those of the CTX-M type, represent a major public health problem throughout the world. Although the carriage of ESBL-producing Enterobacteriaceae (EPE) in feces of horses is now well recognized, little is known about the diversity of EPE after treatment of horses with antibiotics. We undertook this study to assess and follow the diversity of EP Escherichia coli isolated from horses after antibiotic treatment for an infection. Fecal samples from two horses treated and two that were untreated were tested for the presence of EPE on different days...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29775726/pathological-effects-of-concurrent-administration-of-aflatoxin-b-1-and-fowl-adenovirus-4-in-broiler-chicks
#17
Muhammad Noman Naseem, Muhammad Kashif Saleemi, Ahrar Khan, Aisha Khatoon, Shafia Tehseen Gul, Farzana Rizvi, Ijaz Ahmad, Ahad Fayyaz
The current study was designed to investigate pathological effects fowl adenovirus in broilers exposed to aflatoxin B1 . Fowl Adenovirus-4 (FAdV-4) infection is remerging in all types of poultry birds in Pakistan. Poultry feed contamination with mycotoxin (aflatoxin) is another important global issue. A total of 125 a-day old broiler birds were divided into six equal groups. Group A served as control. B and C groups were administered with aflatoxin B1 (AFB1 ) 100 and 200 μg/kg feed. Group D was infected with FAdV-4 while groups E and F administered with both AFB1 (100 & 200 μg/kg) along with FAdV-4...
May 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29775649/expression-and-function-of-tlr4-induced-b1r-bradykinin-receptor-on-cardiac-fibroblasts
#18
Claudia Muñoz-Rodríguez, Samuel Fernández, José Miguel Osorio, Francisco Olivares, Renatto Anfossi, Samir Bolivar, Claudio Humeres, Pía Boza, Raúl Vivar, Viviana Pardo-Jimenez, Karen E Hemmings, Neil A Turner, Guillermo Díaz-Araya
Cardiac fibroblasts (CF) are key cells for maintaining extracellular matrix (ECM) protein homeostasis in the heart, and for cardiac repair through CF-to-cardiac myofibroblast (CMF) differentiation. Additionally, CF play an important role in the inflammatory process after cardiac injury, and they express Toll like receptor 4 (TLR4), B1 and B2 bradykinin receptors (B1R and B2R) which are important in the inflammatory response. B1R and B2R are induced by proinflammatory cytokines and their activation by bradykinin (BK: B2R agonist) or des-arg-kallidin (DAKD: B1R agonist), induces NO and PGI2 production which is key for reducing collagen I levels...
May 15, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29774099/camptothecin-induces-g-2-m-phase-arrest-through-the-atm-chk2-cdc25c-axis-as-a-result-of-autophagy-induced-cytoprotection-implications-of-reactive-oxygen-species
#19
Rajapaksha Gedara Prasad Tharanga Jayasooriya, Matharage Gayani Dilshara, Ilandarage Menu Neelaka Molagoda, Cheol Park, Sang Rul Park, Seungheon Lee, Yung Hyun Choi, Gi-Young Kim
In the present study, we report that camptothecin (CPT) caused irreversible cell cycle arrest at the G2 /M phase, and was associated with decreased levels of cell division cycle 25C (Cdc25C) and increased levels of cyclin B1, p21, and phospho-H3. Interestingly, the reactive oxygen species (ROS) inhibitor, glutathione, decreased CPT-induced G2 /M phase arrest and moderately induced S phase arrest, indicating that the ROS is required for the regulation of CPT-induced G2 /M phase arrest. Furthermore, transient knockdown of nuclear factor-erythroid 2-related factor 2 (Nrf2), in the presence of CPT, increased the ROS' level and further shifted the cell cycle from early S phase to the G2 /M phase, indicating that Nrf2 delayed the S phase in response to CPT...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29774077/long-noncoding-rna-mir503hg-a-prognostic-indicator-inhibits-tumor-metastasis-by-regulating-the-hnrnpa2b1-nf-%C3%AE%C2%BAb-pathway-in-hepatocellular-carcinoma
#20
Hui Wang, Linhui Liang, Qiongzhu Dong, Lin Huan, Jia He, Botai Li, Chen Yang, Haojie Jin, Lin Wei, Chengtao Yu, Fangyu Zhao, Jinjun Li, Ming Yao, Wenxin Qin, Lunxiu Qin, Xianghuo He
Long noncoding RNAs (lncRNAs) have been associated with hepatocellular carcinoma (HCC), but the underlying molecular mechanisms of their specific association with hepatocarcinogenesis have not been fully explored. Methods: miR503HG was identified by microarray and validated by real-time PCR. Survival analysis was evaluated using the Kaplan-Meier method and assessed using the log-rank test. In vitro and in vivo assays were preformed to explore the biological effects of miR503HG in HCC cells. The interaction of miR503HG with HNRNPA2B1 was identified by RNA pull-down and RNA immunoprecipitation...
2018: Theranostics
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