keyword
MENU ▼
Read by QxMD icon Read
search

Human trafficking

keyword
https://www.readbyqxmd.com/read/29470517/two-essential-thioredoxins-mediate-apicoplast-biogenesis-protein-import-and-gene-expression-in-toxoplasma-gondii
#1
Marco Biddau, Anne Bouchut, Jack Major, Tracy Saveria, Julie Tottey, Ojore Oka, Marcel van-Lith, Katherine Elizabeth Jennings, Jana Ovciarikova, Amy DeRocher, Boris Striepen, Ross Frederick Waller, Marilyn Parsons, Lilach Sheiner
Apicomplexan parasites are global killers, being the causative agents of diseases like toxoplasmosis and malaria. These parasites are known to be hypersensitive to redox imbalance, yet little is understood about the cellular roles of their various redox regulators. The apicoplast, an essential plastid organelle, is a verified apicomplexan drug target. Nuclear-encoded apicoplast proteins traffic through the ER and multiple apicoplast sub-compartments to their place of function. We propose that thioredoxins contribute to the control of protein trafficking and of protein function within these apicoplast compartments...
February 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29469805/c-ebp%C3%AE-drives-interactions-between-human-mait-cells-and-endothelial-cells-that-are-important-for-extravasation
#2
Chang Hoon Lee, Hongwei H Zhang, Satya P Singh, Lily Koo, Juraj Kabat, Hsinyi Tsang, Tej Pratap Singh, Joshua M Farber
Many mediators and regulators of extravasation by bona fide human memory-phenotype T cells remain undefined. Mucosal-associated invariant T (MAIT) cells are innate-like, anti-bacterial cells that we found excelled at crossing inflamed endothelium. They displayed abundant selectin ligands, with high expression of FUT7 and ST3GAL4 , and expressed CCR6, CCR5, and CCR2, which played non-redundant roles in trafficking on activated endothelial cells. MAIT cells selectively expressed CCAAT/enhancer-binding protein delta (C/EBPδ)...
February 22, 2018: ELife
https://www.readbyqxmd.com/read/29468680/proinflammatory-role-of-blister-fluid-derived-exosomes-in-bullous-pemphigoid
#3
Hui Fang, Shuai Shao, Man Jiang, Erle Dang, Shengxian Shen, Jieyu Zhang, Pei Qiao, Caixia Li, Gang Wang
Bullous pemphigoid is an autoimmune inflammatory disorder characterised by the presence of autoantibodies against bullous pemphigoid autoantigens, leading to dermal-epidermal separation with consequent blister formation. However, whether and how the components of blister fluid exacerbate the progression of bullous pemphigoid is unclear. Exosomes are nanometre-sized vesicles released from cells into the body fluid, where they can transmit signals throughout the body. In the present study, we isolated and characterised exosomes from the blister fluids of patients with bullous pemphigoid, evaluated their proinflammatory role, and identified the underlying molecular mechanisms...
February 22, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29467750/the-peroxygenase-activity-of-the-aspergillus-flavus-caleosin-afpxg-modulates-the-biosynthesis-of-aflatoxins-and-their-trafficking-and-extracellular-secretion-via-lipid-droplets
#4
Abdulsamie Hanano, Mari Alkara, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Mehedi Hassan, Denis J Murphy
Aflatoxins (AF) are highly detrimental to human and animal health. We recently demonstrated that the Aspergillus flavus caleosin, AfPXG, had peroxygenase activity and mediated fungal development and AF accumulation. We now report the characterization of an AfPXG -deficient line using reference strain NRRL3357. The resulting fungal phenotype included a severe decrease in mycelium growth, failure to sporulate, and reduced AF production. Increasing cellular oxidative status by administration of hydrogen peroxide and cumene hydroperoxide did not restore the AfPXG -deficient phenotype, which suggests that AfPXG -deficiency is not directly related to oxidative stress...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29467617/quantitative-proteomics-of-synaptosomal-fractions-in-a-rat-overexpressing-human-disc1-gene-indicates-profound-synaptic-dysregulation-in-the-dorsal-striatum
#5
Fernando J Sialana, An-Li Wang, Benedetta Fazari, Martina Kristofova, Roman Smidak, Svenja V Trossbach, Carsten Korth, Joseph P Huston, Maria A de Souza Silva, Gert Lubec
Disrupted-in-schizophrenia 1 (DISC1) is a key protein involved in behavioral processes and various mental disorders, including schizophrenia and major depression. A transgenic rat overexpressing non-mutant human DISC1, modeling aberrant proteostasis of the DISC1 protein, displays behavioral, biochemical and anatomical deficits consistent with aspects of mental disorders, including changes in the dorsal striatum, an anatomical region critical in the development of behavioral disorders. Herein, dorsal striatum of 10 transgenic DISC1 (tgDISC1) and 10 wild type (WT) littermate control rats was used for synaptosomal preparations and for performing liquid chromatography-tandem mass spectrometry (LC-MS)-based quantitative proteomics, using isobaric labeling (TMT10plex)...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29464032/stroma-derived-extracellular-vesicles-deliver-tumor-suppressive-mirnas-to-pancreatic-cancer-cells
#6
Song Han, David H Gonzalo, Michael Feely, Carlos Rinaldi, Sayali Belsare, Haiyan Zhai, Krishan Kalra, Michael H Gerber, Christopher E Forsmark, Steven J Hughes
The biology of tumor-associated stroma (TAS) in pancreatic ductal adenocarcinoma (PDAC) is not well understood. The paradoxical observation that stroma-depletion strategies lead to progression of PDAC reinforced the need to critically evaluate the functional contribution of TAS in the initiation and progression of PDAC. PDAC and TAS cells are unique in their expression of specific miRNAs, and this specific miRNA expression pattern alters host to tumor microenvironment interactions. Using primary human pancreatic TAS cells and primary xenograft PDAC cells co-culture, we provide evidence of miRNA trafficking and exchanging between TAS and PDAC cells, in a two-way, cell-contact independent fashion, via extracellular vesicles (EVs) transportation...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29463289/strain-dependent-effects-of-clinical-echovirus-30-outbreak-isolates-at-the-blood-csf-barrier
#7
Tobias Dahm, Ortwin Adams, Sindy Boettcher, Sabine Diedrich, Vasily Morozov, Grant Hansman, Petra Fallier-Becker, Sebastian Schädler, Claus J Burkhardt, Christel Weiss, Carolin Stump-Guthier, Hiroshi Ishikawa, Horst Schroten, Christian Schwerk, Tobias Tenenbaum, Henriette Rudolph
BACKGROUND: Echovirus (E) 30 (E-30) meningitis is characterized by neuroinflammation involving immune cell pleocytosis at the protective barriers of the central nervous system (CNS). In this context, infection of the blood-cerebrospinal fluid barrier (BCSFB), which has been demonstrated to be involved in enteroviral CNS pathogenesis, may affect the tight junction (TJ) and adherens junction (AJ) function and morphology. METHODS: We used an in vitro human choroid plexus epithelial (HIBCPP) cell model to investigate the effect of three clinical outbreak strains (13-311, 13-759, and 14-397) isolated in Germany in 2013, and compared them to E-30 Bastianni...
February 20, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29459263/hyperlipidemia-induces-typical-atherosclerosis-development-in-ldlr-and-apoe-deficient-rats
#8
Yongliang Zhao, Yiqing Yang, Roumei Xing, Xueqin Cui, Yufang Xiao, Ling Xie, Panpan You, Tongtong Wang, Li Zeng, Wenhui Peng, Dali Li, Huaqing Chen, Mingyao Liu
BACKGROUND AND AIMS: Low-density lipoprotein receptor (Ldlr) and apolipoprotein E (Apoe) knockout (KO) mice have been widely used as animal models of atherosclerosis. However, data suggested that it is difficult to develop typical atherosclerosis in rats. To this end, Ldlr and Apoe KO rats were generated and the potential to develop novel atherosclerosis models was evaluated. METHODS: We established Apoe/Ldlr single and double KO (DKO) rats via the CRISPR/Cas9 system in the same background...
February 12, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29457782/hypothalamic-er-associated-degradation-regulates-pomc-maturation-feeding-and-age-associated-obesity
#9
Geun Hyang Kim, Guojun Shi, Diane Rm Somlo, Leena Haataja, Soobin Song, Qiaoming Long, Eduardo A Nillni, Malcolm J Low, Peter Arvan, Martin G Myers, Ling Qi
Pro-opiomelanocortin (POMC) neurons function as key regulators of metabolism and physiology by releasing prohormone-derived neuropeptides with distinct biological activities. However, our understanding of early events in prohormone maturation in the ER remains incomplete. Highlighting the significance of this gap in knowledge, a single POMC cysteine-to-phenylalanine mutation at position 28 (POMC-C28F) is defective for ER processing and causes early onset obesity in a dominant-negative manner in humans through an unclear mechanism...
February 19, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29450149/human-and-mouse-microarrays-guided-expression-analysis-of-membrane-protein-trafficking-related-genes-in-mdck-cells-a-canine-epithelial-model-for-apical-and-basolateral-differential-protein-targeting
#10
Xiaofan Xu, Mingming Pan, Alexis E Gasiewicz, Rongzi Li, Shiu-Ming Kuo
MDCK cells are widely used to study the differential targeting of membrane transporters to apical and basolateral membrane but its canine origin limited the commercial tools available for the analysis of protein trafficking machinery. Because apical and basolateral membranes are only found in differentiated epithelial cells, genes critical for differential targeting may be specifically up-regulated upon MDCK cell differentiation. To search for these genes, a cross-species screening strategy was used. We first analyzed the human microarray data for protein trafficking-related genes that were up-regulated in colon carcinoma Caco2 cells upon differentiation...
June 2017: Biochimie Open
https://www.readbyqxmd.com/read/29447473/impact-of-micronutrients-on-the-immune-response-of-animals
#11
Allen D Smith, Kiran S Panickar, Joseph F Urban, Harry D Dawson
Vitamins and minerals (micronutrients) play an important role in regulating and shaping an immune response. Deficiencies generally result in inadequate or dysregulated cellular activity and cytokine expression, thereby affecting the immune response. Decreased levels of natural killer, granulocyte, and phagocytic cell activity and T and B cell proliferation and trafficking are associated with inadequate levels of micronutrients, as well as increased susceptibility to various adverse health conditions, including inflammatory disorders, infection, and altered vaccine efficacy...
February 15, 2018: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/29447005/human-asic1a-mediates-stronger-acid-induced-responses-as-compared-with-mouse-asic1a
#12
Yuanyuan Xu, Yu-Qing Jiang, Ce Li, Mindi He, W George Rusyniak, Naga Annamdevula, Juan Ochoa, Silas J Leavesley, Jiangping Xu, Thomas C Rich, Mike T Lin, Xiang-Ming Zha
Acid-sensing ion channels (ASICs) are the major proton receptor in the brain and a key mediator of acidosis-induced neuronal injuries in disease. Most of published data on ASIC function came from studies performed in mice, and relatively little is known about potential differences between human and mouse ASICs (hASIC and mASIC, respectively). This information is critical for us to better interpret the functional importance of ASICs in human disease. Here, we examined the expression of ASICs in acutely resected human cortical tissue...
February 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29443946/a-comparative-study-of-human-saposins
#13
María Garrido-Arandia, Bruno Cuevas-Zuviría, Araceli Díaz-Perales, Luis F Pacios
Saposins are small proteins implicated in trafficking and loading of lipids onto Cluster of Differentiation 1 (CD1) receptor proteins that in turn present lipid antigens to T cells and a variety of T-cell receptors, thus playing a crucial role in innate and adaptive immune responses in humans. Despite their low sequence identity, the four types of human saposins share a similar folding pattern consisting of four helices linked by three conserved disulfide bridges. However, their lipid-binding abilities as well as their activities in extracting, transporting and loading onto CD1 molecules a variety of sphingo- and phospholipids in biological membranes display two striking characteristics: a strong pH-dependence and a structural change between a compact, closed conformation and an open conformation...
February 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29443872/ap-1-kif13a-blocking-peptides-impair-melanosome-maturation-and-melanin-synthesis
#14
Cécile Campagne, Léa Ripoll, Floriane Gilles-Marsens, Graça Raposo, Cédric Delevoye
Melanocytes are specialized cells that generate unique organelles called melanosomes in which melanin is synthesized and stored. Melanosome biogenesis and melanocyte pigmentation require the transport and delivery of melanin synthesizing enzymes, such as tyrosinase and related proteins (e.g., TYRP1), from endosomes to maturing melanosomes. Among the proteins controlling endosome-melanosome transport, AP-1 together with KIF13A coordinates the endosomal sorting and trafficking of TYRP1 to melanosomes. We identify here β1-adaptin AP-1 subunit-derived peptides of 5 amino acids that block the interaction of KIF13A with AP-1 in cells...
February 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29439717/dysregulated-phosphorylation-of-rab-gtpases-by-lrrk2-induces-neurodegeneration
#15
Ga Ram Jeong, Eun-Hae Jang, Jae Ryul Bae, Soyoung Jun, Ho Chul Kang, Chi-Hu Park, Joo-Ho Shin, Yukio Yamamoto, Keiko Tanaka-Yamamoto, Valina L Dawson, Ted M Dawson, Eun-Mi Hur, Byoung Dae Lee
BACKGROUND: Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial and sporadic Parkinson's disease (PD). Elevated kinase activity is associated with LRRK2 toxicity, but the substrates that mediate neurodegeneration remain poorly defined. Given the increasing evidence suggesting a role of LRRK2 in membrane and vesicle trafficking, here we systemically screened Rab GTPases, core regulators of vesicular dynamics, as potential substrates of LRRK2 and investigated the functional consequence of such phosphorylation in cells and in vivo...
February 13, 2018: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/29439330/hypoxia-induced-signaling-activation-in-neurodegenerative-diseases-targets-for-new-therapeutic-strategies
#16
Niraj Kumar Jha, Saurabh Kumar Jha, Renu Sharma, Dhiraj Kumar, Rashmi K Ambasta, Pravir Kumar
For the maintenance of cellular homeostasis and energy metabolism, an uninterrupted supply of oxygen (O2) is routinely required in the brain. However, under the impaired level of O2 (hypoxia) or reduced blood flow (ischemia), the tissues are not sufficiently oxygenated, which triggers disruption of cellular homeostasis in the brain. Hypoxia is known to have a notable effect on controlling the expression of proteins involved in a broad range of biological processes varying from energy metabolism, erythropoiesis, angiogenesis, neurogenesis to mitochondrial trafficking and autophagy, thus facilitating neuronal cells to endure in deprived O2...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29437871/gut-microbiota-modulate-t-cell-trafficking-into-human-colorectal-cancer
#17
Eleonora Cremonesi, Valeria Governa, Jesus Francisco Glaus Garzon, Valentina Mele, Francesca Amicarella, Manuele Giuseppe Muraro, Emanuele Trella, Virginie Galati-Fournier, Daniel Oertli, Silvio Raffael Däster, Raoul A Droeser, Benjamin Weixler, Martin Bolli, Raffaele Rosso, Ulrich Nitsche, Nina Khanna, Adrian Egli, Simone Keck, Julia Slotta-Huspenina, Luigi M Terracciano, Paul Zajac, Giulio Cesare Spagnoli, Serenella Eppenberger-Castori, Klaus-Peter Janssen, Lubor Borsig, Giandomenica Iezzi
OBJECTIVE: Tumour-infiltrating lymphocytes (TILs) favour survival in human colorectal cancer (CRC). Chemotactic factors underlying their recruitment remain undefined. We investigated chemokines attracting T cells into human CRCs, their cellular sources and microenvironmental triggers. DESIGN: Expression of genes encoding immune cell markers, chemokines and bacterial 16S ribosomal RNA (16SrRNA) was assessed by quantitative reverse transcription-PCR in fresh CRC samples and corresponding tumour-free tissues...
February 6, 2018: Gut
https://www.readbyqxmd.com/read/29437135/human-trafficking
#18
EDITORIAL
Amy C Brown, Christine E Barron
No abstract text is available yet for this article.
February 2018: Pediatrics in Review
https://www.readbyqxmd.com/read/29435408/a-putative-rna-binding-protein-from-plasmodium-vivax-apicoplast
#19
Sofía M García-Mauriño, Antonio Díaz-Quintana, Francisco Rivero-Rodríguez, Isabel Cruz-Gallardo, Christian Grüttner, Marian Hernández-Vellisca, Irene Díaz-Moreno
Malaria is caused by Apicomplexa protozoans from the Plasmodium genus entering the bloodstream of humans and animals through the bite of the female mosquitoes. The annotation of the Plasmodium vivax genome revealed a putative RNA binding protein (apiRBP) that was predicted to be trafficked into the apicoplast, a plastid organelle unique to Apicomplexa protozoans. Although a 3D structural model of the apiRBP corresponds to a noncanonical RNA recognition motif with an additional C-terminal α-helix (α3), preliminary protein production trials were nevertheless unsuccessful...
February 2018: FEBS Open Bio
https://www.readbyqxmd.com/read/29434999/functions-of-nuclear-actin-binding-proteins-in-human-cancer
#20
Xinyi Yang, Ying Lin
Nuclear actin-binding proteins (ABPs) perform distinguishable functions compared with their cytoplasmic counterparts in extensive activities of living cells. In addition to the ability to regulate actin cytoskeleton dynamics, nuclear ABPs are associated with multiple nuclear biological processes, including chromatin remodeling, gene transcriptional regulation, DNA damage response, nucleocytoplasmic trafficking and nuclear structure maintenance. The nuclear translocation of ABPs is affected by numerous intracellular or extracellular stimuli, which may lead to developmental malformation, tumor initiation, tumor progression and metastasis...
March 2018: Oncology Letters
keyword
keyword
12843
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"