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https://www.readbyqxmd.com/read/29345345/autophagy-inhibition-promotes-phagocytosis-of-macrophage-and-protects-mice-from-methicillin-resistant-staphylococcus-aureus-pneumonia
#1
Yulin Zhu, Huichun Li, Shenggang Ding, Yating Wang
OBJECTIVE: To investigate the effect of autophagy in macrophages and the protection of mouse models against MRSA invasion, which provide new potential therapeutic direction for lung infection. METHODS: The effect of ST239 in macrophages were analyzed by western blot and immunofluorescence to observe the influence of autophagy inhibitor 3-MA in macrophages. Then we established MRSA mice model and the models were divided into different groups of drugs. As well as analyzed the effect of autophagy in macrophage with MRSA and the changes of lung pathological in the mouse model by flow cytometry and immunohistochemistry...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29344647/autophagy-regulates-the-degeneration-of-the-auditory-cortex-through-the-ampk-mtor-ulk1-signaling-pathway
#2
Jie Yuan, Xueyan Zhao, Yujuan Hu, Haiying Sun, Guoqing Gong, Xiang Huang, Xubo Chen, Mingyu Xia, Chen Sun, Qilin Huang, Yu Sun, Wen Kong, Weijia Kong
Presbycusis is the most common sensory impairment associated with aging; however, the underlying molecular mechanism remains unclear. Autophagy has been demonstrated to serve a key role in diverse diseases; however, no studies have examined its function in central presbycusis. The aim of the present study was to investigate the changes of autophagy in the physiological processes of the auditory cortex and its role in the degeneration of the auditory cortex, as well as the related mechanisms using naturally aging rats and a D‑galactose (D‑gal)‑induced mimetic rat model of aging...
January 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29344231/expression-of-beclin-1-and-bcl-2-in-pancreatic-neoplasms-and-its-effect-on-pancreatic-ductal-adenocarcinoma-prognosis
#3
Shanshan Song, Baosheng Wang, Shuailin Gu, Xiaocheng Li, Shaolong Sun
Aberrant expression of Beclin 1 and B-cell lymphoma-2 (Bcl-2) has been identified in a variety of human tumors; however, little information is available for pancreatic neoplasms. The present study analyzed the expression of Beclin 1 and Bcl-2 in pancreatic ductal adenocarcinoma (PDAC) and solid pseudopapillary neoplasm (SPN) of the pancreas, and evaluated their prognostic significance for PDAC. The present study included 117 PDAC, 43 SPN and 32 chronic pancreatitis (CP) cases. Levels of Beclin 1 and Bcl-2 expression were evaluated semiquantitatively by immunohistochemistry, and their correlation with the survival of patients with PDAC was determined...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344152/regulation-of-autophagy-inhibition-and-inflammatory-response-in-glioma-by-wnt-signaling-pathway
#4
Tongxin Wang, Zhixia Chen, Wei Zhang
The objective of this study was to investigate the mechanism of the function of Wnt signaling pathway in regulating autophagy and inflammatory response in glioma cells. Human brain glioma cells U118 were selected and divided into three groups: i) the Wnt signaling inhibitor IWR-1 group (the observation group); ii) the PBS negative control group (the PBS group) and iii) the blank control group. After 24 h culture, Wnt5a/β-catenin protein, autophagy marker, microtubule-associated-proteins-1A1B-light-chain-3C (LC-3) II and Beclin I, and inflammatory factors IL-6 and TNF-α protein expression levels were evaluated using western blotting...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344024/elevated-phosphate-levels-trigger-autophagy-mediated-cellular-apoptosis-in-h9c2-cardiomyoblasts
#5
Yao-Lung Liu, Kuan-Ho Lin, Shanmugam Tamilselvi, Wei-Kung Chen, Chia-Yao Shen, Ray-Jade Chen, Cecilia Hsuan Day, Hsi-Chin Wu, Vijaya Padma Viswanadha, Chih-Yang Huang
BACKGROUND/AIM: In chronic kidney disease (CKD), kidneys fail to maintain phosphorus homeostasis in serum. Elevated phosphorus levels in serum have been associated with cardiovascular diseases in CKD patients and in normal individuals. In this study, we evaluated the level of autophagy- and apoptosis-related markers under different concentrations of hyperphosphate in myocardial cells. METHODS: Modulation inflicted on the levels of various survival-, autophagy-, and apoptosis-related markers were determined by Western blotting analysis using total protein extract...
December 2017: Cardiorenal Medicine
https://www.readbyqxmd.com/read/29340072/hyperbaric-oxygen-protects-against-myocardial-reperfusion-injury-via-the-inhibition-of-inflammation-and-the-modulation-of-autophagy
#6
Chunxia Chen, Wan Chen, Yaoxuan Li, Yanling Dong, Xiaoming Teng, Zhihuan Nong, Xiaorong Pan, Liwen Lv, Ying Gao, Guangwei Wu
Our previous study demonstrated that hyperbaric oxygen (HBO) preconditioning protected against myocardial ischemia reperfusion injury (MIRI) and improved myocardial infarction. However, HBO's effect on MIRI-induced inflammation and autophagy remains unclear. In this study, we investigate the potential impact and underlying mechanism of HBO preconditioning on an MIRI-induced inflammatory response and autophagy using a ligation of the left anterior descending (LAD) coronary artery rat model. Our results showed that HBO restored myocardial enzyme levels and decreased the apoptosis of cardiomyocytes, which were induced by MIRI...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29339018/nicotine-alleviates-chronic-stress-induced-anxiety-and-depressive-like-behavior-and-hippocampal-neuropathology-via-regulating-autophagy-signaling
#7
Xi Xiao, Xueliang Shang, Baohui Zhai, Hui Zhang, Tao Zhang
Recently, we reported that chronic nicotine significantly improved chronic stress-induced impairments of cognition and the hippocampal synaptic plasticity in mice, however, the underlying mechanism still needs to be explored. In the present study, 32 male C57BL/6 mice were divided into four groups: control (CON), stress (CUS), stress with chronic nicotine administration (CUS + Nic) and chronic nicotine administration (Nic). The anxiety-like behavior and neuropathological alteration of DG neurons were examined...
January 12, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29335016/autophagy-is-required-for-human-umbilical-cord-mesenchymal-stem-cells-to-improve-spatial-working-memory-in-app-ps1-transgenic-mouse-model
#8
Wen Li, Kai Li, Jing Gao, Zhuo Yang
BACKGROUND: Recent studies have shown that autophagy plays a central role in mesenchymal stem cells (MSCs), and many studies have shown that human umbilical cord MSCs (huMSCs) can treat Alzheimer's disease (AD) through a variety of mechanisms. However, no studies have looked at the effects of autophagy on neuroprotective function of huMSCs in the AD mouse model. Thus, in this study we investigated whether inhibition of autophagy could weaken or block the function of huMSCs through in vitro and in vivo experiments...
January 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29334671/glucosamine-promotes-osteoblast-proliferation-by-modulating-autophagy-via-the-mammalian-target-of-rapamycin-pathway
#9
Chen Lv, Lu Wang, Xiongbai Zhu, Wenjun Lin, Xin Chen, Zhengxiang Huang, Lintuo Huang, Shengwu Yang
Glucosamine is effective in the treatment of osteoarthritis; however, its effect on osteoporosis remains unclear. Decreased activity of osteoblasts is the main cause of osteoporosis. Here, we examined the effects of glucosamine on osteoblasts. The potential underlying mechanisms were explored. The results showed that glucosamine had a biphasic effect on the viability of hFOB1.19 osteoblasts. At low concentrations (<0.6 mM), glucosamine induced hFOB1.19 cell proliferation, whereas at high concentrations (>0...
January 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29333604/adiponectin-inhibits-inflammatory-cytokines-production-by-beclin-1-phosphorylation-and-bcl-2-mrna-destabilization-role-for-autophagy-induction
#10
Nirmala Tilija Pun, Pil-Hoon Park
BACKGROUND AND PURPOSE: Adiponectin potently suppresses inflammatory mediator production. Autophagy is known to play a critical role in modulating inflammatory responses by adiponectin. However, the underlying mechanisms are not clearly understood. Interaction between Beclin-1 and Bcl-2 is a critical event in autophagy induction. We examined the effects of globular adiponectin (gAcrp) on the Beclin-1/Bcl-2 association and its underlying mechanisms. EXPERIMENTAL APPROACH: The effect of gAcrp on the interaction between Beclin-1 and Bcl-2 was examined by immunoprecipitation followed by Western blotting...
January 14, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29331511/maresin-1-regulates-autophagy-and-inflammation-in-human-periodontal-ligament-cells-through-glycogen-synthase-kinase-3%C3%AE-%C3%AE-catenin-pathway-under-inflammatory-conditions
#11
Li Du, Yucheng Li, Weifeng Liu
OBJECTIVE: Accumulating lines of evidence suggest that maresin 1 (MaR-1) exerts anti-inflammatory effects in many cell types and plays beneficial roles in inflammatory disease, such as peritonitis and colitis. Moreover, it has been demonstrated that MaR-1 play protective roles against localized aggressive periodontitis. However, the function and mechanism of MaR-1 in human periodontal ligament cells (PDL) cells from periodontitis are poorly understood. The present study aimed to clarify the effects and molecular mechanism of MaR-1 in PDL cell survival and inflammation...
December 23, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29328494/pterostilbene-inhibits-reactive-oxygen-species-production-and-apoptosis-in-primary-spinal-cord-neurons-by-activating-autophagy-via-the-mechanistic-target-of-rapamycin-signaling-pathway
#12
Jing-Lan He, Xiao-Hui Dong, Zong-Hu Li, Xiao-Ying Wang, Zhi-An Fu, Na Shen
Autophagy is an important self-adaptive mechanism that is involved in inhibiting reactive oxygen species (ROS) in spinal cord neurons. Pterostilbene, a natural plant extract, has been demonstrated to possess antioxidant effects; however, it has not yet been investigated whether pterostilbene could activate autophagy and protect spinal cord neurons from oxidative stress. In the present study, primary spinal cord neurons of Sprague Dawley rats were cultured. Cell counting kit‑8 analysis was used to detect cytotoxicity of pterostilbene...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328447/high-mobility-group-protein%C3%A2-b1-silencing-promotes-susceptibility-of-retinoblastoma-cells-to-chemotherapeutic-drugs-through-downregulating-nuclear-factor-%C3%AE%C2%BAb
#13
Yong Chai, Juhua Xiao, Shouhua Zhang, Yunyan Du, Zhipeng Luo, Xin Zhou, Kai Huang
The aim of the present study was to investigate the effects of high-mobility group protein B1 (HMGB1) silencing on the susceptibility of retinoblastoma (RB) cells to chemotherapeutic drugs and the underlying molecular mechanisms. Western blot analysis revealed that vincristine (VCR), etoposide (ETO) and carboplatin (CBP) significantly increased the expression of HMGB1 in Weri‑Rb-1 and Y79 cells compared with the untreated control (P<0.01). siRNA HMGB1 and siRNA negative control (NC) were transfected to Y79 cells by Lipofectamine™ 2000 and, following VCR treatment, the expression of HMGB1 and nuclear factor-κB (NF-κB) was analyzed...
January 10, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29325369/-cd137-signaling-promotes-the-formation-of-plaque-calcification-via-inhibiting-the-fusion-of-autophagy-and-lysosomal-in-apo-e-mice
#14
X Y Li, R Chen, W Zhong, B Li, C Shao, Z Q Wang, J C Yan
Objective: To investigate whether CD137 signaling promoted the formation of atherosclerotic plaque calcification by inhibiting the fusion of autophagosome and lysosome. Methods: (1) In vivo, CD137 agonist antibody and anti-CD137 antibody were used to stimulate and inhibit the CD137 signaling, respectively. Fifteen Apo E(-/-) mice were randomly divided into three groups: control group (intraperitoneal injection of IgG2b 200 µg) , CD137 agonist group (intraperitoneal injection of CD137 agonist antibody 200 µg) , anti-CD137 group (pretreatment with 200 µg anti-CD137 antibody for 24 hours, then injection of CD137 agonist antibody) ...
December 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29323257/dengue-virus-induced-er-stress-is-required-for-autophagy-activation-viral-replication-and-pathogenesis-both-in-vitro-and-in-vivo
#15
Ying-Ray Lee, Szu-Han Kuo, Ching-Yen Lin, Po-Jung Fu, Yee-Shin Lin, Trai-Ming Yeh, Hsiao-Sheng Liu
Dengue virus (DENV) utilizes the endoplasmic reticulum (ER) for replication and assembling. Accumulation of unfolded proteins in the ER lumen leads to ER stress and unfolded protein response (UPR). Three branches of UPRs temporally modulated DENV infection. Moreover, ER stress can also induce autophagy. DENV infection induces autophagy which plays a promotive role in viral replication has been reported. However, the role of ER stress in DENV-induced autophagy, viral titer, and pathogenesis remain unclear. Here, we reveal that ER stress and its downstream UPRs are indispensable for DENV-induced autophagy in various human cells...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321496/genistein-promotes-ionizing-radiation-induced-cell-death-by-reducing-cytoplasmic-bcl-xl-levels-in-non-small-cell-lung-cancer
#16
Zhimin Zhang, Feng Jin, Xiaojuan Lian, Mengxia Li, Ge Wang, Baohua Lan, Hao He, Guo-Dong Liu, Yan Wu, Guiyin Sun, Cheng-Xiong Xu, Zhen-Zhou Yang
Genistein (GEN) has been previously reported to enhance the radiosensitivity of cancer cells; however, the detailed mechanisms remain unclear. Here, we report that GEN treatment inhibits the cytoplasmic distribution of Bcl-xL and increases nuclear Bcl-xL in non-small cell lung cancer (NSCLC). Interestingly, our in vitro data show that ionizing radiation IR treatment significantly increases IR-induced DNA damage and apoptosis in a low cytoplasmic Bcl-xL NSCLC cell line compared to that of high cytoplasmic Bcl-xL cell lines...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29318850/-effect-of-moxibustion-preconditioning-with-seed-sized-moxa-cones-on-myocardial-infarction-size-and-beclin-1-expression-in-myocardial-ischemia-reperfusion-injury-rats
#17
Hua Bai, Sheng-Feng Lu, Wan-Ying Chen, Ze-Hao Zhong, Yi-Huang Gu
OBJECTIVE: To observe the effect of moxibustion (Moxi) preconditioning with seed-sized moxa cones on myocardial ischemia-reperfusion injury (MI/RI) at different stages, and to analyze the correlation between this effect and the expression of autophagy related protein Beclin 1. METHODS: This study contains two parts: 1) changes of myocardial pathological injury and percentages of myocardial infarcted area at different time-points after modeling and Moxi intervention, and 2) effect of Moxi on contents of serum cardiac troponin T(cTnT) and expression of myocardial Bcl-2, Bax and Beclin 1 proteins...
December 25, 2017: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29316373/tanshinone-iia-induces-cell-death-via-beclin-1-dependent-autophagy-in-oral-squamous-cell-carcinoma-scc-9-cell-line
#18
Ye Qiu, Conghua Li, Qinhua Wang, Xingqi Zeng, Ping Ji
Tanshinone IIA (TAN) is one of the major functional compounds of Salvia miltiorrhiza Bunge and possesses the ability to suppress the growth of multiple cancer cell types via its apoptosis- and autophagy-inducing functions. In this study, the effect of TAN therapy on the survival of oral squamous cell carcinoma (OSCC) was evaluated, and the underlying mechanism involved in the treatment was investigated. Human oral squamous cell carcinoma cell SCC-9 was used for in vitro assays and induction in an OSCC xenograft mouse model...
January 6, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29314656/mammalian-target-of-rapamycin-inhibition-attenuates-myocardial-ischaemia-reperfusion-injury-in-hypertrophic-heart
#19
Lei-Lei Ma, Xin Ma, Fei-Juan Kong, Jun-Jie Guo, Hong-Tao Shi, Jian-Bing Zhu, Yun-Zeng Zou, Jun-Bo Ge
Pathological cardiac hypertrophy aggravated myocardial infarction and is causally related to autophagy dysfunction and increased oxidative stress. Rapamycin is an inhibitor of serine/threonine kinase mammalian target of rapamycin (mTOR) involved in the regulation of autophagy as well as oxidative/nitrative stress. Here, we demonstrated that rapamycin ameliorates myocardial ischaemia reperfusion injury by rescuing the defective cytoprotective mechanisms in hypertrophic heart. Our results showed that chronic rapamycin treatment markedly reduced the phosphorylated mTOR and ribosomal protein S6 expression, but not Akt in both normal and aortic-banded mice...
January 4, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29313410/ulk1-phosphorylates-ser30-of-becn1-in-association-with-atg14-to-stimulate-autophagy-induction
#20
Ji-Man Park, Minchul Seo, Chang Hwa Jung, Douglas Grunwald, Matthew Stone, Neil Michael Otto, Erik Toso, Yeseul Ahn, Michael Kyba, Timothy J Griffin, LeeAnn Higgins, Do-Hyung Kim
ULK1 (unc51-like autophagy activating kinase 1) is a serine/threonine kinase that plays a key role in regulating macroautophagy/autophagy induction in response to amino acid starvation. Despite the recent progress in understanding ULK1 functions, the molecular mechanism by which ULK1 regulates the induction of autophagy remains elusive. In this study, we determined that ULK1 phosphorylates Ser30 of BECN1 (beclin 1) in association with ATG14 (autophagy related 14) but not with UVRAG (UV radiation resistance associated)...
January 9, 2018: Autophagy
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