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https://www.readbyqxmd.com/read/28735485/genome-wide-analysis-of-dna-methylation-in-hematopoietic-cells-dna-methylation-analysis-by-wgbs
#1
Mira Jeong, Anna G Guzman, Margaret A Goodell
DNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735416/super-resolution-microscopical-localization-of-dopamine-receptors-1-and-2-in-rat-hippocampal-synaptosomes
#2
Andras G Miklosi, Giorgia Del Favero, Tanja Bulat, Harald Höger, Ryuichi Shigemoto, Doris Marko, Gert Lubec
Although dopamine receptors D1 and D2 play key roles in hippocampal function, their synaptic localization within the hippocampus has not been fully elucidated. In order to understand precise functions of pre- or postsynaptic dopamine receptors (DRs), the development of protocols to differentiate pre- and postsynaptic DRs is essential. So far, most studies on determination and quantification of DRs did not discriminate between subsynaptic localization. Therefore, the aim of the study was to generate a robust workflow for the localization of DRs...
July 22, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28735414/bone-mechanobiology-in-mice-toward-single-cell-in-vivo-mechanomics
#3
Ariane Scheuren, Esther Wehrle, Felicitas Flohr, Ralph Müller
Mechanically driven bone (re)modeling is a multiscale process mediated through complex interactions between multiple cell types and their microenvironments. However, the underlying mechanisms of how cells respond to mechanical signals are still unclear and are at the focus of the field of bone mechanobiology. Traditionally, this complex process has been addressed by reducing the system to single scales and cell types. It is only recently that more integrative approaches have been established to study bone mechanobiology across multiple scales in which mechanical load at the organ level is related to molecular responses at the cellular level...
July 22, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28735394/detection-of-differential-dna-methylation-under-stress-conditions-using-bisulfite-sequence-analysis
#4
Ibtisam Al Harrasi, Rashid Al-Yahyai, Mahmoud W Yaish
DNA methylation is the most important epigenetic change affecting gene expression in plants grown under normal as well as under stress conditions. Therefore, researchers study differential DNA methylation under distinct environmental conditions and their relationship with transcriptome abundance. Up to date, more than 25 methods and techniques are available to detect DNA methylation based on different principles. Bisulfite sequencing method is considered as a gold standard since it is able to distinguish 5-methylcytosine from cytosine using the bisulfite treatment...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735282/a-systematic-review-of-criteria-used-to-define-recovery-from-sport-related-concussion-in-youth-athletes
#5
REVIEW
Mohammad Nadir Haider, John J Leddy, Sonja Pavlesen, Melissa Kluczynski, John G Baker, Jeffrey C Miecznikowski, Barry S Willer
OBJECTIVE: The Concussion in Sport Group guidelines recommend a multifaceted approach to help clinicians make return to sport decisions. The purpose of this study was to identify the most common multifaceted measures used to define clinical recovery from sport-related concussion in young athletes (high school and/or college level) and to summarise existing knowledge of criteria used to make return to sport decisions. DESIGN: Systematic review. DATA SOURCES: The PubMed (MEDLINE), SPORTDiscus and Embase electronic databases were searched from 1 January 2000 to 1 March 2017 by three independent reviewers...
July 22, 2017: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/28735216/elastic-properties-and-strain-to-crack-initiation-of-calcium-phosphate-bone-cements-revelations-of-a-high-resolution-measurement-technique
#6
Ingrid Ajaxon, Alice Acciaioli, Giacomo Lionello, Maria-Pau Ginebra, Caroline Öhman-Mägi, Massimiliano Baleani, Cecilia Persson
Calcium phosphate cements (CPCs) should ideally have mechanical properties similar to those of the bone tissue the material is used to replace or repair. Usually, the compressive strength of the CPCs is reported and, more rarely, the elastic modulus. Conversely, scarce or no data are available on Poisson's ratio and strain-to-crack-initiation. This is unfortunate, as data on the elastic response is key to, e.g., numerical model accuracy. In this study, the compressive behaviour of brushite, monetite and apatite cements was fully characterised...
June 21, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28735215/estimation-of-individual-axon-bundle-properties-by-a-multi-resolution-discrete-search-method
#7
Ricardo Coronado-Leija, Alonso Ramirez-Manzanares, Jose Luis Marroquin
A stable, accurate and robust-to-noise method for the estimation of the intra-voxel bundle-wise diffusion properties for diffusion-weighted magnetic resonance imaging is presented. The proposed method overcomes some of the limitations of most of the multi-fiber algorithms in the literature and extends them to estimate the diffusion profiles, improving the estimation of the intra-voxel geometry at challenging microstructure configurations, that is to say: relatively small crossing angles, different voxel-wise anisotropic diffusion profiles and low SNR...
June 29, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28735122/ventral-lumbar-synovial-cyst-causing-cauda-equina-compression-case-report-and-literature-review
#8
Anan Shtaya, Ahmed-Ramadan Sadek, Mark Walker, Ali Nader-Sepahi
BACKGROUND: Juxtafacet spinal cysts are cystic synovial lesions that are often indistinguishable clinically or radiologically and require histopathology analysis to confirm the diagnosis. Lumbar synovial cysts usually arising from the synovium of the facet joints. They have been described posterolateral or rarely in the posterior midline. However, we describe the first synovial cyst ventral to the dural sac. CASE DESCRIPTION: We report a lumbar 3-4 lesion causing cauda equina compression in a 57-year-old man who presented with 3 months history of low back pain and bilateral sciatica, intermittent urinary incontinence and erectile dysfunction...
July 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28735094/dedicated-tool-to-assess-the-impact-of-a-rhetorical-task-on-human-body-temperature
#9
Robert Koprowski, Sławomir Wilczyński, Katarzyna Martowska, Dominik Gołuch, Emilia Wrocławska-Warchala
Functional infrared thermal imaging is a method widely used in medicine, including analysis of the mechanisms related to the effect of emotions on physiological processes. The article shows how the body temperature may change during stress associated with performing a rhetorical task and proposes new parameters useful for dynamic thermal imaging measurements MATERIALS AND METHODS: 29 healthy male subjects were examined. They were given a rhetorical task that induced stress. Analysis and processing of collected body temperature data in a spatial resolution of 256×512pixels and a temperature resolution of 0...
July 19, 2017: International Journal of Psychophysiology
https://www.readbyqxmd.com/read/28735057/brazil-s-resolutions-to-regulate-the-sale-of-antibiotics-impact-on-consumption-and-escherichia-coli-resistance-rates
#10
Karen P H Mattos, Marília B Visacri, Júlia C F Quintanilha, Gustavo R Lloret, Maria A Cursino, Anna S Levin, Carlos E Levy, Patricia Moriel
OBJECTIVE: The aim of this study was to determine the impact of two resolutions to restrict antibiotic use (RDCs no. 44/2010 and 20/2011) in the Campinas metropolitan area (Sao Paulo, Brazil) on antibiotic consumption, resistance rates, and trends in Escherichia coli-causing community-acquired urinary tract infection (UTI). METHODS: The annual retail sale information of antibiotics from drugstores in the Campinas metropolitan area between 2008 and 2012 were obtained through the Intercontinental Medical Statistics Health of Brazil...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28735011/assessment-and-elimination-of-the-effects-of-head-movement-on-meg-resting-state-measures-of-oscillatory-brain-activity
#11
Eirini Messaritaki, Loes Koelewijn, Diana C Dima, Gemma M Williams, Gavin Perry, Krish D Singh
Magnetoencephalography (MEG) is increasingly being used to study brain function because of its excellent temporal resolution and its direct association with brain activity at the neuronal level. One possible cause of error in the analysis of MEG data comes from the fact that participants, even MEG-experienced ones, move their head in the MEG system. Head movement can cause source localization errors during the analysis of MEG data, which can result in the appearance of source variability that does not reflect brain activity...
July 19, 2017: NeuroImage
https://www.readbyqxmd.com/read/28734976/structural-basis-of-the-substrate-specificity-and-instability-in-solution-of-a-glycosidase-from-lactobacillus-plantarum
#12
Iván Acebrón, Laura Plaza-Vinuesa, Blanca de Las Rivas, Rosario Muñoz, J Cumella, F Sánchez-Sancho, José Miguel Mancheño
Statistics from structural genomics initiatives reveal that around 50-55% of the expressed, non-membrane proteins cannot be purified and therefore structurally characterized due to solubility problems, which emphasized protein solubility as one of the most serious concerns in structural biology projects. Lactobacillus plantarum CECT 748(T) produces an aggregation-prone glycosidase (LpBgl) that we crystallized previously. However, this result could not be reproduced due to protein instability and therefore further high-resolution structural analyses of LpBgl were impeded...
July 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28734975/how-consumers-are-affected-by-product-descriptions-in-online-shopping-event-related-potentials-evidence-of-the-attribute-framing-effect
#13
Jia Jin, Wuke Zhang, Mingliang Chen
Due to the limitations of the human ability to process information, e-consumers' decisions are likely to be influenced by various cognitive biases, such as the attribute framing effect. This effect has been well studied by numerous scholars; however, the associated underlying neural mechanisms with a critical temporal resolution have not been revealed. Thus, this study applies the measurement of event-related potentials (ERPs) to directly examine the role of attribute framing in information processing and decision-making in online shopping...
July 19, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28734895/a-sensitive-and-convenient-method-for-clinical-detection-of-non-syndromic-hearing-loss-associated-common-mutations
#14
Er-Feng Yuan, Wei Xia, Jing-Tao Huang, Ling Hu, Xing Liao, Xiang Dai, Song-Mei Liu
BACKGROUND: The majority of non-syndromic hearing loss (NSHL) patients result from causative mutations in GJB2, SLC26A4 and mitochondrial 12S rRNA genes. Accurate detection of these genetic mutations is increasingly recognized for its clinical significance to reduce incidence and guide individual treatment of NSHL. Current methods for clinical practice are labor intensive, expensive or of low sensitivity. METHODS: Genomic DNA from 7 newborns not passing the hearing screening and 94 newborns passing the hearing screening were analyzed for the common mutations using high resolution melting analysis (HRMA) and Sanger sequencing...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28734843/a-high-resolution-melting-hrm-technology-based-assay-for-cost-efficient-clinical-detection-and-genotyping-of-herpes-simplex-virus-hsv-1-and-hsv-2
#15
M Lieveld, A Carregosa, I Benoy, N Redzic, M Berth, D Vanden Broeck
Genital herpes can be caused by two very similar viruses, herpes simplex virus (HSV)-1 or HSV-2. These two HSV types cannot be distinguished clinically, but genotyping is recommended in the first-episodes of genital herpes to guide counselling and management. Quantitative polymerase chain reaction (qPCR) is the preferred diagnostic method for HSV typing. However, commercial qPCR methods use expensive fluorescent labeled probes for detection. Furthermore, most low-cost methods are not able to differentiate between HSV-1 and -2...
July 19, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28734839/recombinant-expression-of-ixolaris-a-kunitz-type-inhibitor-from-the-tick-salivary-gland-for-nmr-studies
#16
V S De Paula, F H S Silva, I M B Francischetti, R Q Monteiro, A P Valente
Ixolaris is an anticoagulant protein identified in the tick saliva of Ixodes scapularis. Ixolaris contains 2 Kunitz like domains and binds to Factor Xa or Factor X as a scaffold for inhibition of the Tissue Factor (TF)/Factor VIIa (FVIIa). In contrast to tissue factor pathway inhibitor (TFPI), however, Ixolaris does not bind to the active site cleft of FXa. Instead, complex formation is mediated by the FXa heparin-binding exosite. Due to its potent and long-lasting antithrombotic activity, Ixolaris is a promising agent for anticoagulant therapy...
July 19, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28734351/capillary-electrophoresis-tandem-mass-spectrometry-as-a-highly-selective-tool-for-the-compositional-and-site-specific-assessment-of-multiple-peptide-deamidation
#17
Elena Dominguez-Vega, Thomas De Vijlder, Edwin P Romijn, Govert W Somsen
Site-specific mapping of multiple deamidations in peptides is a challenging analytical task. In this work, capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) is presented as a high-resolution tool for the detailed characterization of these subtle modifications in peptides. The 4.5-kDa peptide drug TRI-1144, which contains five closely-positioned potential deamidation sites, was selected as model compound. TRI-1144 was exposed to acidic conditions and/or elevated temperatures for 1-14 h. Stressed samples were analyzed using a background electrolyte (BGE) of 150 mM ammonium formate (pH 6...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734350/dried-urine-spots-a-novel-sampling-technique-for-comprehensive-lc-ms-n-drug-screening
#18
Julian A Michely, Markus R Meyer, Hans H Maurer
Dried matrix spot (DMS) technique as alternative sampling strategy, especially dried urine spots (DUS), might be an alternative for drug screening. So far only particular drugs or drug classes were covered in DMS screenings. Therefore, workup of DUS for a broad comprehensive library-based LC-MS(n) screening was developed. It consisted of enzymatic on-spot deconjugation followed by liquid extraction and LC-MS(n) analysis. This workup was compared to established urine precipitation (UP) and validated according to international guidelines for qualitative approaches, using exemplary compounds of several drug classes (antidepressants, benzodiazepines, cardiovascular drugs, neuroleptics, opioids, stimulants, etc...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734253/polybrominated-diphenyl-ether-flame-retardant-concentrations-in-faeces-from-young-children-in-queensland-australia-and-associations-with-environmental-and-behavioural-factors
#19
Karin English, Yiqin Chen, Leisa-Maree Toms, Paul Jagals, Robert S Ware, Jochen F Mueller, Peter D Sly
The aim of our study was to investigate children's exposure to the flame retardants polybrominated diphenyl ethers (PBDEs) by analysing faecal content, a non-invasive matrix, as well as responses to an exposure-assessment questionnaire. A convenience sample of 61 parents with children (aged >3 months to <2 years) completed an online pre-tested questionnaire and provided faecal samples for analysis by high resolution gas chromatography/mass spectrometry. BDE-209 was the dominant congener in faecal samples adjusted to 8...
July 19, 2017: Environmental Research
https://www.readbyqxmd.com/read/28734112/fused-multivariate-empirical-mode-decomposition-memd-and-inverse-solution-method-for-eeg-source-localization
#20
Pegah Khosropanah, Abdul Rahman Ramli, Kheng Seang Lim, Mohammad Hamiruce Marhaban, Anvarjon Ahmedov
EEG source localization is determining possible cortical sources of brain activities with scalp EEG. Generally, every step of the data processing sequence affects the accuracy of EEG source localization. In this paper, we introduce a fused multivariate empirical mode decomposing (MEMD) and inverse solution algorithm with an embedded unsupervised eye blink remover in order to localize the epileptogenic zone accurately. For this purpose, we constructed realistic forward models using MRI and boundary element method (BEM) for each patient to obtain results that are more realistic...
July 22, 2017: Biomedizinische Technik. Biomedical Engineering
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