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https://www.readbyqxmd.com/read/28351011/fine-cutting-edge-shaped-bi2o3rods-reduced-graphene-oxide-rgo-composite-for-supercapacitor-and-visible-light-photocatalytic-applications
#1
D Maruthamani, S Vadivel, M Kumaravel, B Saravanakumar, Bappi Paul, Siddhartha Sankar Dhar, Aziz Habibi-Yangjeh, A Manikandan, Govindarajan Ramadoss
Bi2O3 rods/RGO composite has been synthesized by a simple precipitation and calcination method. The crystallnity, structural, and morphological features were studied by X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), and high resolution transmission electron microscopy (HR-TEM) techniques. The supercapacitor behavior was studied using cyclic voltammetry, galvanostatic charge discharge and impedance analysis, respectively. The Bi2O3 rods/RGO nanocomposite exhibits a maximum specific capacitance of 1041Fg(-1) at a current density of 2Ag(-1)...
March 22, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28350988/micrornas-establish-uniform-traits-during-the-architecture-of-vertebrate-embryos
#2
Dionna M Kasper, Albertomaria Moro, Emma Ristori, Anand Narayanan, Guillermina Hill-Teran, Elizabeth Fleming, Miguel Moreno-Mateos, Charles E Vejnar, Jing Zhang, Donghoon Lee, Mengting Gu, Mark Gerstein, Antonio Giraldez, Stefania Nicoli
Proper functioning of an organism requires cells and tissues to behave in uniform, well-organized ways. How this optimum of phenotypes is achieved during the development of vertebrates is unclear. Here, we carried out a multi-faceted and single-cell resolution screen of zebrafish embryonic blood vessels upon mutagenesis of single and multi-gene microRNA (miRNA) families. We found that embryos lacking particular miRNA-dependent signaling pathways develop a vascular trait similar to wild-type, but with a profound increase in phenotypic heterogeneity...
March 27, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28350960/enhanced-stability-of-magnetic-skyrmion-lattice-phase-under-a-tilted-magnetic-field-in-two-dimensional-chiral-magnet
#3
Chao Wang, Haifeng Du, Xuebing Zhao, Chiming Jin, Mingliang Tian, Yuheng Zhang, Renchao Che
Magnetic skyrmion is a topologically stable vortex-like spin texture that offers great promise as information carriers for future spintronic devices. In a two-dimensional chiral magnet, it was generally considered that a tilted magnetic field is harmful to its formation and stability. Here we investigated the angular dependent stability of magnetic skyrmions in FeGe nanosheets by using high-resolution Lorentz transmission electron microscopy (Lorentz TEM). Besides the theoretically predicted destruction of skyrmion lattice state by an oblique magnetic field as temperature close to its magnetic Curie temperature Tc~278 K, we also observed an unexpected reentry-like phenomenon at the moderate temperatures near the border between conical and skyrmion phase,Tt~240 K...
March 28, 2017: Nano Letters
https://www.readbyqxmd.com/read/28350891/an-on-chip-instrument-for-white-blood-cells-classification-based-on-a-lens-less-shadow-imaging-technique
#4
Yuan Fang, Ningmei Yu, Runlong Wang, Dong Su
Routine blood tests provide important basic information for disease diagnoses. The proportions of three subtypes of white blood cells (WBCs), which are neutrophils, monocytes, lymphocytes, is key information for disease diagnosis. However, current instruments for routine blood tests, such as blood cell analyzers, flow cytometers, and optical microscopes, are cumbersome, time consuming and expensive. To make a smaller, automatic low-cost blood cell analyzer, much research has focused on a technique called lens-less shadow imaging, which can obtain microscopic images of cells in a lens-less system...
2017: PloS One
https://www.readbyqxmd.com/read/28350888/patterns-of-variation-in-diversity-of-the-mississippi-river-microbiome-over-1-300-kilometers
#5
Jason T Payne, Justin J Millar, Colin R Jackson, Clifford A Ochs
We examined the downriver patterns of variation in taxonomic diversity of the Mississippi River bacterioplankton microbiome along 1,300 river kilometers, or approximately one third the total length of the river. The study section included portions of the Upper, Middle, and Lower Mississippi River, confluences with five tributaries draining distinct sub-basins, river cities, and extended stretches without major inputs to the Mississippi. The composition and proportional abundance of dominant bacterial phyla was distinct for free-living and particle-associated cells, and constant along the entire reach, except for a substantial but transient disturbance near the city of Memphis, Tennessee...
2017: PloS One
https://www.readbyqxmd.com/read/28350824/time-and-radiation-dose-dependent-changes-in-the-plasma-proteome-after-total-body-irradiation-of-non-human-primates-implications-for-biomarker-selection
#6
Stephanie D Byrum, Marie S Burdine, Lisa Orr, Samuel G Mackintosh, Simon Authier, Mylene Pouliot, Martin Hauer-Jensen, Alan J Tackett
Acute radiation syndrome (ARS) is a complex multi-organ disease resulting from total body exposure to high doses of radiation. Individuals can be exposed to total body irradiation (TBI) in a number of ways, including terrorist radiological weapons or nuclear accidents. In order to determine whether an individual has been exposed to high doses of radiation and needs countermeasure treatment, robust biomarkers are needed to estimate radiation exposure from biospecimens such as blood or urine. In order to identity such candidate biomarkers of radiation exposure, high-resolution proteomics was used to analyze plasma from non-human primates following whole body irradiation (Co-60 at 6...
2017: PloS One
https://www.readbyqxmd.com/read/28350655/high-resolution-magnetic-resonance-imaging-of-aging-upper-face-fat-compartments
#7
Rémi Foissac, Olivier Camuzard, Sébastien Piereschi, Pascal Staccini, Olivier Andreani, Charalambos Georgiou, Marc Benatar
BACKGROUND: Age-related changes in fat compartments have yet to be fully characterized. Uncertainty remains in terms of volume gains/losses or shape fluctuations over time. The authors' aim was to determine the evolution of subcutaneous fat in the aging upper face, focusing on shifts in volume and dimension. METHODS: Over the course of 4.5 years, 100 faces of living female Caucasian patients were prospectively studied using high-resolution magnetic resonance imaging...
April 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28350523/comparison-of-micro-ct-and-cone-beam-ct-on-the-feasibility-of-assessing-mandibular-trabecular-structures-in-mandibular-condyle
#8
Xin Liang, Zuyan Zhang, Jianping Gu, Zhihui Wang, Bart Vandenberghe, Reinhilde Jacobs, Jie Yang, Guowu Ma, Haibin Ling, Xuchen Ma
OBJECTIVES: To evaluate the accuracy of Cone-beam CT (CBCT) in assessing trabecular structures Methods: Two human mandibles were scanned by micro-CT (Skyscan 1173 high energy spiral scan micro-CT, Skyscan NV, Kontich, Belgium) and CBCT(3D Accuitomo 170, Morita Japan). The CBCT images were reconstructed in 0.5mm and 1 mm thickness. The condylar images were selected for registration. A parallel algorithm for histogram computation was introduced to perform the registration. Mutual information (MI) value was used to evaluate the match between the images obtained from micro-CT and CBCT...
March 28, 2017: Dento Maxillo Facial Radiology
https://www.readbyqxmd.com/read/28350468/3d-proton-beam-writing-of-optically-active-coherent-vacancy-spins-in-silicon-carbide
#9
Hannes Kraus, Dmitrij Simin, Christian Kasper, Yoshinori Suda, Shunsuke Kawabata, Wataru Kada, Tomoya Honda, Yasuto Hijikata, Takeshi Ohshima, Vladimir Dyakonov, Georgy V Astakhov
Constructing quantum devices comprises various challenging tasks, especially when concerning their nanoscale geometry. For quantum color centers, the traditional approach is to fabricate the device structure after the non-deterministic placement of the centers. Reversing this approach, we present the controlled generation of quantum centers in silicon carbide (SiC) by focused proton beam in a non-complex manner without need for pre- or post-irradiation treatment. The generation depth and resolution can be predicted by matching the proton energy to the material's stopping power, and the amount of quantum centers at one specific sample volume is tuneable from ensembles of millions to discernible single photon emitters...
March 28, 2017: Nano Letters
https://www.readbyqxmd.com/read/28350444/bottom-up-elucidation-of-glycosidic-bond-stereochemistry
#10
Christopher John Gray, Baptiste Schindler, Lukasz G Migas, Martina Picmanova, Abdul-Rahman Allouche, Anthony P Green, Santanu Mandal, Mohammed Saddik Motawia, Raquel Sánchez-Pérez, Nanna Bjarnholt, Birger Lindberg Møller, Anouk M Rijs, Perdita E Barran, Isabelle Compagnon, Claire E Eyers, Sabine L Flitsch
The lack of robust, high-throughput and sensitive analytical strategies that can conclusively map the structure of glycans has significantly hampered progress in fundamental and applied aspects of glycoscience. Resolution of the anomeric α/β glycan linkage within oligosaccharides remains a particular challenge. Here, we show that 'memory' of anomeric configuration is retained following gas-phase glycosidic bond fragmentation during tandem mass spectrometry (MS2). These findings allow for integration of MS2 with ion mobility spectrometry (IM-MS2) and lead The to a strategy to distinguish α- and β-linkages within natural underivatised carbohydrates...
March 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28346887/high-resolution-mass-spectrometry-as-an-alternative-detection-method-to-tandem-mass-spectrometry-for-the-analysis-of-endogenous-steroids-in-serum
#11
Federico Ponzetto, Julien Boccard, Norbert Baume, Tiia Kuuranne, Serge Rudaz, Martial Saugy, Raul Nicoli
Recently, steroid hormones quantification in blood showed a promising ability to detect testosterone doping and interesting complementarities with the urinary module of the Athlete Biological Passport (ABP). In this work, an ultra-high pressure liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) method was developed for the quantification of eleven endogenous steroids in serum. The performance of the full scan and targeted SIM acquisition modes was evaluated and compared to the performance of tandem mass spectrometry (MS/MS)...
March 18, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28346884/automatic-measurement-of-melanoma-depth-of-invasion-in-skin-histopathological-images
#12
Hongming Xu, Richard Berendt, Naresh Jha, Mrinal Mandal
Measurement of melanoma depth of invasion (DoI) in skin tissues is of great significance in grading the severity of skin disease and planning patient's treatment. However, accurate and automatic measurement of melanocytic tumor depth is a challenging problem mainly due to the difficulty of skin granular identification and melanoma detection. In this paper, we propose a technique for measuring melanoma DoI in microscopic images digitized from MART1 (i.e., meleanoma-associated antigen recognized by T cells) stained skin histopathological sections...
March 10, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28346882/determination-of-prodrug-treosulfan-and-its-biologically-active-monoepoxide-in-rat-plasma-liver-lungs-kidneys-muscle-and-brain-by-hplc-esi-ms-ms-method
#13
Michał Romański, Anna Kasprzyk, Artur Teżyk, Agnieszka Widerowska, Czesław Żaba, Franciszek Główka
A prodrug treosulfan (TREO) is currently investigated in clinical trials for conditioning prior to hematopoietic stem cell transplantation. Bioanalysis of TREO and its active derivatives, monoepoxide (S,S-EBDM) and diepoxide, in plasma and urine underlay the pharmacokinetic studies of these compounds but cannot explain an organ pharmacological action or toxicity. Recently, distribution of TREO and S,S-EBDM into brain, cerebrospinal fluid, and aqueous humor of the eye has been investigated in animal models and the obtained results presented clinical relevance...
March 18, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28346826/imaging-successive-intermediate-states-of-the-on-surface-ullmann-reaction-on-cu-111-role-of-the-metal-coordination
#14
Sören Zint, Daniel Ebeling, Tobias Schlöder, Sebastian Ahles, Doreen Mollenhauer, Hermann A Wegner, Andre Schirmeisen
The in-depth knowledge about on-surface reaction mechanisms is crucial for the tailor-made design of covalently bonded organic frameworks, for applications such as nanoelectronic or -optical devices. Latest developments in atomic force microscopy, which rely on functionalizing the tip with single CO molecules at low temperatures allow to image molecular systems with submolecular resolution. Here, we are using this technique to study the complete reaction pathway of the on-surface Ullmann-type coupling between bromotriphenylene molecules on a Cu(111) surface...
March 27, 2017: ACS Nano
https://www.readbyqxmd.com/read/28346784/structure-determination-of-mycobacterium-tuberculosis-serine-protease-hip1-rv2224c
#15
Jacqueline Lara Naffin-Olivos, Andrew Daab, Andre White, Nathan Eric Goldfarb, Amy C Milne, Dali Liu, Jacqueline Baikovitz, Ben M Dunn, Jyothi Rengarajan, Gregory A Petsko, Dagmar Ringe
The Mycobacterium tuberculosis (Mtb) serine protease Hip1 (Hydrolase important for pathogenesis; Rv2224c) promotes tuberculosis (TB) pathogenesis by impairing host immune responses through proteolysis of a protein substrate, Mtb GroEL2. The cell surface localization of Hip1 and its immunomodulatory functions make Hip1 a good drug target for new adjunctive immune therapies for TB. Here we report the crystal structure of Hip1 to a resolution of 2.6 Å and the kinetic studies of the enzyme against model substrates and the protein GroEL2...
March 27, 2017: Biochemistry
https://www.readbyqxmd.com/read/28346708/high-resolution-isotopic-monitoring-of-cave-air-co2
#16
Paul Töchterle, Yuri Dublyansky, Nils Stöbener, Magda Mandić, Christoph Spötl
RATIONALE: CO2 is the main driver of many chemical processes in cave environments. Understanding CO2 fluxes in a given cave system through monitoring campaigns has become a standard procedure in a wide variety of fields such as paleoclimatology or show cave management. However, conventional methods lack the resolution of isotopic data to capture many transient processes occurring in caves. METHODS: A novel approach using isotope ratio infrared spectrometry (IRIS) to monitor cave air pCO2 , δ(13) C and δ(18) O values in situ was tested and compared with conventional monitoring methods (handheld pCO2 meter and discrete cave air samples for conventional isotope ratio mass spectrometry)...
March 27, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28346538/discrimination-of-rippled-spectrum-patterns-in-noise-a-manifestation-of-compressive-nonlinearity
#17
Olga N Milekhina, Dmitry I Nechaev, Vladimir O Klishin, Alexander Ya Supin
In normal-hearing listeners, rippled-spectrum discrimination was psychophysically investigated in both silence and with a simultaneous masker background using the following two paradigms: measuring the ripple density resolution with the phase-reversal test and measuring the ripple-shift threshold with the ripple-shift test. The 0.5-oct wide signal was centered on 2 kHz, the signal levels were 50 and 80 dB SPL, and the masker levels varied from 30 to 100 dB SPL. The baseline ripple density resolutions were 8...
2017: PloS One
https://www.readbyqxmd.com/read/28346485/identification-and-discrimination-of-toxoplasma-gondii-sarcocystis-spp-neospora-spp-and-cryptosporidium-spp-by-righ-resolution-melting-analysis
#18
Hllytchaikra Ferraz Fehlberg, Bianca Mendes Maciel, George Rêgo Albuquerque
The objective of this study was to standardize the high-resolution melting method for identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by amplification of 18S ribosomal DNA (rDNA) using a single primer pair. The analyses were performed on individual reactions (containing DNA from a single species of a protozoan), on duplex reactions (containing DNA from two species of protozoa in each reaction), and on a multiplex reaction (containing DNA of four parasites in a single reaction)...
2017: PloS One
https://www.readbyqxmd.com/read/28346456/sub-10-nm-patterning-via-directed-self-assembly-of-block-copolymer-films-with-a-vapour-phase-deposited-topcoat
#19
Hyo Seon Suh, Do Han Kim, Priya Moni, Shisheng Xiong, Leonidas E Ocola, Nestor J Zaluzec, Karen K Gleason, Paul F Nealey
Directed self-assembly (DSA) of the domain structure in block copolymer (BCP) thin films is a promising approach for sub-10-nm surface patterning. DSA requires the control of interfacial properties on both interfaces of a BCP film to induce the formation of domains that traverse the entire film with a perpendicular orientation. Here we show a methodology to control the interfacial properties of BCP films that uses a polymer topcoat deposited by initiated chemical vapour deposition (iCVD). The iCVD topcoat forms a crosslinked network that grafts to and immobilizes BCP chains to create an interface that is equally attractive to both blocks of the underlying copolymer...
March 27, 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28346450/structural-modeling-of-protein-rna-complexes-using-crosslinking-of-segmentally-isotope-labeled-rna-and-ms-ms
#20
Georg Dorn, Alexander Leitner, Julien Boudet, Sébastien Campagne, Christine von Schroetter, Ahmed Moursy, Ruedi Aebersold, Frédéric H-T Allain
Ribonucleoproteins (RNPs) are key regulators of cellular function. We established an efficient approach, crosslinking of segmentally isotope-labeled RNA and tandem mass spectrometry (CLIR-MS/MS), to localize protein-RNA interactions simultaneously at amino acid and nucleotide resolution. The approach was tested on polypyrimidine tract binding protein 1 and U1 small nuclear RNP. Our method provides distance restraints to support integrative atomic-scale structural modeling and to gain mechanistic insights into RNP-regulated processes...
March 27, 2017: Nature Methods
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