keyword
MENU ▼
Read by QxMD icon Read
search

Resolution

keyword
https://www.readbyqxmd.com/read/29793096/segmentation-of-histological-images-and-fibrosis-identification-with-a-convolutional-neural-network
#1
Xiaohang Fu, Tong Liu, Zhaohan Xiong, Bruce H Smaill, Martin K Stiles, Jichao Zhao
Segmentation of histological images is one of the most crucial tasks for many biomedical analyses involving quantification of certain tissue types, such as fibrosis via Masson's trichrome staining. However, challenges are posed by the high variability and complexity of structural features in such images, in addition to imaging artifacts. Further, the conventional approach of manual thresholding is labor-intensive, and highly sensitive to inter- and intra-image intensity variations. An accurate and robust automated segmentation method is of high interest...
May 16, 2018: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/29793080/visualizing-the-shape-of-society-an-analysis-of-public-bads-and-burden-allocation-due-to-household-consumption-using-an-input-output-approach
#2
Andrew Chapman, Yosuke Shigetomi
This study investigates how our lifestyles can cause societal issue including a reduction in social equity due to the consumption of natural resources. Based on a range of household environmental footprints and their application to a quantitative social equity evaluation framework, a methodology is proposed which identifies the creation and origin of public bads within society. This research builds on the methodologies of energy policy sustainability evaluation incorporated with environmentally extended input output analysis in order to critically assess lifestyle-based consumption impacts, and to quantify the allocation of subsequent burdens across generations...
May 21, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29793061/microstructural-imaging-of-the-human-brain-with-a-super-scanner-10-key-advantages-of-ultra-strong-gradients-for-diffusion-mri
#3
D K Jones, D C Alexander, R Bowtell, M Cercignani, F Dell'Acqua, D J McHugh, K L Miller, M Palombo, G J M Parker, U S Rudrapatna, C M W Tax
The key component of a microstructural diffusion MRI 'super-scanner' is a dedicated high-strength gradient system that enables stronger diffusion weightings per unit time compared to conventional gradient designs. This can, in turn, drastically shorten the time needed for diffusion encoding, increase the signal-to-noise ratio, and facilitate measurements at shorter diffusion times. This review, written from the perspective of the National Facility for In Vivo MR Imaging of Human Tissue Microstructure, an initiative to establish a shared 300 mT/m-gradient facility amongst the microstructural imaging community, describes ten advantages of ultra-strong gradients for microstructural imaging...
May 21, 2018: NeuroImage
https://www.readbyqxmd.com/read/29793032/phosphorylated-and-non-phosphorylated-hck-kinase-domains-produced-by-cell-free-protein-expression
#4
Kazushige Katsura, Yuri Tomabechi, Takayoshi Matsuda, Mayumi Yonemochi, Junko Mikuni, Noboru Ohsawa, Takaho Terada, Shigeyuki Yokoyama, Mutsuko Kukimoto-Niino, Chie Takemoto, Mikako Shirouzu
Since phosphorylation is involved in various physiological events, kinases and interacting factors can be potential targets for drug discovery. For the development and improvement of inhibitors from the point of view of mechanistic enzymology, a cell-free protein synthesis system would be advantageous, since it could prepare mutant proteins easily. However, especially in the case of protein kinase, product solubility remains one of the major challenges. To overcome this problem, we prepared a chaperone-supplemented extract from Escherichia coli BL21 cells harboring a plasmid encoding a set of chaperone genes, dnaK, dnaJ, and grpE...
May 21, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29793028/cytosolic-distributions-of-highly-toxic-metals-cd-and-tl-and-several-essential-elements-in-the-liver-of-brown-trout-salmo-trutta-l-analyzed-by-size-exclusion-chromatography-and-inductively-coupled-plasma-mass-spectrometry
#5
Zrinka Dragun, Nesrete Krasnići, Nicol Kolar, Vlatka Filipović Marijić, Dušica Ivanković, Marijana Erk
Cytosolic distributions of nonessential metals Cd and Tl and seven essential elements among compounds of different molecular masses were studied in the liver of brown trout (Salmo trutta) from the karstic Krka River in Croatia. Analyses were done by size exclusion high performance liquid chromatography and high resolution inductively coupled plasma mass spectrometry. Common feature of Cd and Tl, as highly toxic elements, was their distribution within only two narrow peaks. The increase of cytosolic Cd concentrations was reflected in marked increase of Cd elution within low molecular mass peak (maximum at ∼15 kDa), presumably containing metallothioneins (MTs), which indicated successful Cd detoxification in brown trout liver under studied exposure conditions...
May 15, 2018: Chemosphere
https://www.readbyqxmd.com/read/29793023/kinetics-and-stereochemistry-of-linb-catalyzed-%C3%AE-hbcd-transformation-comparison-of-in-vitro-and-in-silico-results
#6
Norbert V Heeb, Manuel Mazenauer, Simon Wyss, Birgit Geueke, Hans-Peter E Kohler, Peter Lienemann
LinB is a haloalkane dehalogenase found in Sphingobium indicum B90A, an aerobic bacterium isolated from contaminated soils of hexachlorocyclohexane (HCH) dumpsites. We showed that this enzyme also converts hexabromocyclododecanes (HBCDs). Here we give new insights in the kinetics and stereochemistry of the enzymatic transformation of δ-HBCD, which resulted in the formation of two pentabromocyclododecanols (PBCDols) as first- (P1δ , P2δ ) and two tetrabromocyclododecadiols (TBCDdiols) as second-generation products (T1δ , T2δ )...
May 12, 2018: Chemosphere
https://www.readbyqxmd.com/read/29792954/increased-brain-docosahexaenoic-acid-has-no-effect-on-the-resolution-of-neuroinflammation-following-intracerebroventricular-lipopolysaccharide-injection
#7
Marc-Olivier Trépanier, Kathryn E Hopperton, Vanessa Giuliano, Mojgan Masoodi, Richard P Bazinet
Resolution of inflammation in the periphery was once thought to be a passive process, but new research now suggests it is an active process mediated by specialized pro-resolving lipid mediators (SPM) derived from omega-3 polyunsaturated fatty acids (n-3 PUFA). However, this has yet to be illustrated in neuroinflammation. The purpose of this study was to measure resolution of neuroinflammation and to test whether increasing brain docosahexaenoic acid (DHA) affects the resolution of neuroinflammation. C57Bl/6 mice, fat-1 mice and their wildtype littermates, fed either fish oil or safflower oil, received lipopolysaccharide (LPS) in the left lateral ventricle...
May 21, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29792820/hydrogel-tissue-chemistry-principles-and-applications
#8
Viviana Gradinaru, Jennifer Treweek, Kristin Overton, Karl Deisseroth
Over the past five years, a rapidly developing experimental approach has enabled high-resolution and high-content information retrieval from intact multicellular animal (metazoan) systems. New chemical and physical forms are created in the hydrogel-tissue chemistry process, and the retention and retrieval of crucial phenotypic information regarding constituent cells and molecules (and their joint interrelationships) are thereby enabled. For example, rich data sets defining both single-cell-resolution gene expression and single-cell-resolution activity during behavior can now be collected while still preserving information on three-dimensional positioning and/or brain-wide wiring of those very same neurons-even within vertebrate brains...
May 20, 2018: Annual Review of Biophysics
https://www.readbyqxmd.com/read/29792807/micelles-protect-intact-metallo-supramolecular-block-copolymer-complexes-from-solution-to-gas-phase-during-electrospray-ionization
#9
Kai Hung Huang, Tsung-Han Tu, Shi-Cheng Wang, Yi-Tsu Chan, Cheng-Chih Hsu
Supramolecular diblock copolymers using metal-ligand coordination can be synthesized under ambient conditions by delicate design of the end groups of the homopolymer chains. However, mass spectrometric analysis of such metallo-supramolecular copolymers is challenging. One of the reasons is the nonpolarity of the polymer chains, making it hard to disperse the complexes in electrospray ionization (ESI)-friendly environments. The other difficulty is the noncovalent nature of such copolymers, which is easily disrupted during the ionization...
May 24, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29792746/high-resolution-ct-findings-of-obstructive-and-restrictive-phenotypes-of-chronic-lung-allograft-dysfunction-more-than-just-bronchiolitis-obliterans-syndrome
#10
Partha Hota, Chandra Dass, Maruti Kumaran, Scott Simpson
OBJECTIVE: The purpose of this article is to review the high-resolution CT characteristics of individual obstructive and restrictive chronic lung allograft dysfunction (CLAD) phenotypes to aid in making accurate diagnoses and guiding treatment. CONCLUSION: Long-term survival and function after lung transplant are considerably worse compared with after other organ transplants. CLAD is implicated as a major limiting factor for long-term graft viability. Historically thought to be a single entity, bronchiolitis obliterans syndrome, CLAD is actually a heterogeneous group of disorders with distinct subtypes...
May 24, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29792720/-experimental-processing-of-corrosion-casts-of-large-animal-organs
#11
R Pálek, V Liška, L Eberlová, H Mírka, M Svoboda, S Haviar, M Emingr, O Brzoň, P Mik, V Třeška
INTRODUCTION: Corrosion casts (CCs) are used for the visualization and assessment of hollow structures. CCs with filled capillaries enable (with the help of imaging methods) to obtain data for mathematical organ perfusion modelling. As the processing is more difficult in case of organs with greater volume of the vasculature, mainly organs from small animals have been cast up to now. The aim of this study was to optimize the protocol of corrosion casting of different organs of pig. Porcine organs are relatively easily accessible and frequently used in experimental medicine...
2018: Rozhledy V Chirurgii: Měsíčník Československé Chirurgické Společnosti
https://www.readbyqxmd.com/read/29792690/a-general-method-to-increase-stokes-shift-by-introducing-alternating-vibronic-structures
#12
Tian-Bing Ren, Wang Xu, Wei Zhang, Xing-Xing Zhang, Zhi-Yao Wang, Zhen Xiang, Lin Yuan, Xiaobing Zhang
Fluorescent dyes have enabled the progress of the broad biomedical fields. However, many commercially available dyes suffer from small Stokes shifts, resulting in poor signal-to-noise ratio and self-quenching on current microscope configuration. In this work, we have developed a general method to significantly increase the Stokes shifts of common fluorophores. By simply appending a 1,4-diethyl-decahydro-quinoxaline (DQ) moiety onto the conjugated structure, we introduced a vibronic backbone that could facilely expand the Stokes shifts, emission wavelength and photo-stability of eleven different fluorophores by more than 3-fold...
May 24, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29792674/partial-intrinsic-disorder-governs-the-dengue-capsid-protein-conformational-ensemble
#13
Priscilla L S Boon, Wuan Geok Saw, Xin Xiang Lim, Palur Venkata Raghuvamsi, Roland G Huber, Jan K Marzinek, Daniel A Holdbrook, Ganesh S Anand, Gerhard Grüber, Peter J Bond
The 11 kDa, positively charged dengue capsid protein (C protein) exists stably as a homodimer and co-localizes with the viral genome within mature viral particles. Its core is composed of four alpha helices encompassing a small hydrophobic patch that may interact with lipids, but approximately 20% of the protein at the N-terminus is intrinsically disordered, making it challenging to elucidate its conformational landscape. Here, we combine small-angle X-ray scattering (SAXS), amide hydrogen-deuterium exchange mass spectrometry (HDXMS), and atomic-resolution molecular dynamics (MD) simulations to probe the dynamics of dengue C proteins...
May 24, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29792462/extreme-teams-toward-a-greater-understanding-of-multiagency-teamwork-during-major-emergencies-and-disasters
#14
Nicola Power
Major emergencies are extreme team decision making environments. They are complex, dynamic, high-stakes and fast paced events, wherein successful resolution is contingent upon effective teamwork. Not only do emergency teams coordinate at the intrateam level (e.g., police team), but they are increasingly required to operate at the interteam level (e.g., police, fire and ambulance teams). This is in response to the desire for networked and cost-effective practice and due to the evolving nature of modern threats, such as extreme weather events and terrorist attacks, which require a multi- rather than single-agency response...
May 2018: American Psychologist
https://www.readbyqxmd.com/read/29792422/toward-total-synthesis-of-thiolate-protected-metal-nanoclusters
#15
Qiaofeng Yao, Tiankai Chen, Xun Yuan, Jianping Xie
Total synthesis, where desired organic- and/or biomolecules could be produced from simple precursors at atomic precision and with known step-by-step reactions, has prompted centuries-lasting bloom of organic chemistry since its conceptualization in 1828 (Wöhler synthesis of urea). Such expressive science is also highly desirable in nanoscience, since it represents a decisive step toward atom-by-atom customization of nanomaterials for basic and applied research. Although total synthesis chemistry is less established in nanoscience, recent years have witnessed seminal advances and increasing research efforts devoted into this field...
May 24, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29792421/high-temperature-structural-evolution-in-the-ba-3-mo-1-x-w-x-nbo-8-5-system-and-correlation-with-ionic-transport-properties
#16
Andrea Bernasconi, Cristina Tealdi, Lorenzo Malavasi
The evolution of the hybrid structure between 9R hexagonal perovskite and palmierite in the entire Ba3 Mo(1- x) W x NbO8.5 solid solution (where x = 0, 0.25, 0.5, 0.75, and 1) was probed in the 100-900 K range by synchrotron high-resolution powder diffraction. Each sample exhibits a chemical-dependent structural model in the low-temperature regime (from 100 to 500 K) in which 9R and palmierite structures compete each other, the former being progressively favored as tungsten replaces molybdenum. Above 500 K, unit cell parameters and metal site occupancies start to converge toward a similar structural arrangement that is completely reached at 900 K...
May 24, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29792390/mental-health-crisis-resolution-teams-and-crisis-care-systems-in-england-a-national-survey
#17
Brynmor Lloyd-Evans, Danielle Lamb, Joseph Barnby, Michelle Eskinazi, Amelia Turner, Sonia Johnson
Aims and methodA national survey investigated the implementation of mental health crisis resolution teams (CRTs) in England. CRTs were mapped and team managers completed an online survey. RESULTS: Ninety-five per cent of mapped CRTs (n = 233) completed the survey. Few CRTs adhered fully to national policy guidelines. CRT implementation and local acute care system contexts varied substantially. Access to CRTs for working-age adults appears to have improved, compared with a similar survey in 2012, despite no evidence of higher staffing levels...
May 24, 2018: BJPsych Bulletin
https://www.readbyqxmd.com/read/29792332/ligation-enhanced-%C3%AF-hole%C3%A2-%C3%A2-%C3%A2-%C3%AF-interactions-involving-isocyanides-effect-of-%C3%AF-hole%C3%A2-%C3%A2-%C3%A2-%C3%AF-noncovalent-bonding-on-conformational-stabilization-of-acyclic-diaminocarbene-ligands
#18
Alexander S Mikherdov, Mikhail A Kinzhalov, Alexander S Novikov, Vadim P Boyarskiy, Irina A Boyarskaya, Margarita S Avdontceva, Vadim Yu Kukushkin
The reaction of cis-[PdCl2 (CNXyl)2 ] (Xyl = 2,6-Me2 C6 H3 ) with the aminoazoles [1 H-imidazol-2-amine (1), 4 H-1,2,4-triazol-3-amine (2), 1 H-tetrazol-5-amine (3), 1 H-benzimidazol-2-amine (4), 1-alkyl-1 H-benzimidazol-2-amines, where alkyl = Me (5), Et (6)] in a 2:1 ratio in the presence of a base in CHCl3 at RT proceeds regioselectively and leads to the binuclear diaminocarbene complexes [(ClPdCNXyl)2 {μ-C(N-azolyl)N(Xyl)C═NXyl}] (7-12; 73-91%). Compounds 7-12 were characterized by C, H, N elemental analyses, high-resolution ESI+ -MS, Fourier transform infrared spectroscopy, 1D (1 H, 13 C) and 2D (1 H,1 H-COSY, 1 H,1 H-NOESY, 1 H,13 C-HSQC, 1 H,13 C-HMBC) NMR spectroscopies, and X-ray diffraction (XRDn)...
May 24, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29792325/non-geminal-aliphatic-dihalogenation-pattern-in-dichlorinated-diaporthins-from-hamigera-fusca-nrrl-35721
#19
Celso Almeida, Ignacio Pérez-Victoria, Víctor González-Menéndez, Nuria de Pedro, Jesús Martín, Gloria Crespo, Thomas Mackenzie, Bastien Cautain, Fernando Reyes, Francisca Vicente, Olga Genilloud
Two new epimeric dihalogenated diaporthins, (9 R * )-8-methyl-9,11-dichlorodiaporthin (2) and (9 S * )-8-methyl-9,11-dichlorodiaporthin (3), have been isolated from the soil fungus Hamigera fusca NRRL 35721 alongside the known regioisomeric isocoumarin 8-methyl-11,11-dichlorodiaporthin (1). Their structures were elucidated by high-resolution mass spectrometry and NMR spectroscopy combined with molecular modeling. Compounds 1-3 are the first isocoumarins and the first halogenated metabolites ever reported from the Hamigera genus...
May 24, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/29792308/logical-modelling-uncovers-developmental-constraints-for-primary-sex-determination-of-chicken-gonads
#20
Lucas Sánchez, Claudine Chaouiya
In the chicken, sex determination relies on a ZZ (male)/ZW (female) chromosomal system, but underlying mechanisms are still not fully understood. The Z-dosage and the dominant W-chromosome hypotheses have been proposed to underlie primary sex determination. We present a modelling approach, which assembles the current knowledge and permits exploration of the regulation of this process in chickens. Relying on published experimental data, we assembled a gene network, which led to a logical model that integrates both the Z-dosage and dominant W hypotheses...
May 2018: Journal of the Royal Society, Interface
keyword
keyword
12826
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"