keyword
MENU ▼
Read by QxMD icon Read
search

Resolution

keyword
https://www.readbyqxmd.com/read/28646743/analysis-of-ictal-magnetoencephalography-using-gradient-magnetic-field-topography-gmft-in-patients-with-neocortical-epilepsy
#1
Hiroshi Shirozu, Akira Hashizume, Hiroshi Masuda, Yosuke Ito, Yoko Nakayama, Takefumi Higashijima, Masafumi Fukuda, Shigeki Kameyama
OBJECTIVE: We aimed to validate the usefulness of gradient magnetic-field topography (GMFT) for analysis of ictal magnetoencephalography (MEG) in patients with neocortical epilepsy. METHODS: We identified 13 patients presenting with an ictal event during preoperative MEG. We applied equivalent current dipole (ECD) estimation and GMFT to detect and localize the ictal MEG onset, and compared these methods with the ictal onset zone (IOZ) derived from chronic intracranial electroencephalography...
June 7, 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/28646694/elimination-of-signals-tilting-caused-by-b0-field-inhomogeneity-using-2d-lineshape-reference-deconvolution
#2
Kirill F Sheberstov, Dmitry O Sinitsyn, Dmitry A Cheshkov, Damien Jeannerat
An efficient approach for reference deconvolution of two-dimensional spectra aiming at the correction of static field inhomogeneity was established. In comparison to known techniques, a great improvement was achieved using the cross-section along the diagonal of the reference peak instead of its full 2D line shape. The method is termed pseudo-2D diagonal deconvolution. The approach developed allows suppressing the two-dimensional peaks tilting caused by the magnetic field inhomogeneity, while keeping the signal-to-noise ratio constant...
June 8, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28646659/analysis-of-recombinant-monoclonal-antibodies-in-hydrophilic-interaction-chromatography-a-generic-method-development-approach
#3
Balázs Bobály, Valentina D'Atri, Alain Beck, Davy Guillarme, Szabolcs Fekete
Hydrophilic interaction liquid chromatography (HILIC) is a well-established technique for the separation and analysis of small polar compounds. A recently introduced widepore stationary phase expanded HILIC applications to larger molecules, such as therapeutic proteins. In this paper, we present some generic HILIC conditions adapted for a wide range of FDA and EMA approved recombinant monoclonal antibody (mAb) species and for an antibody-drug conjugate (ADC). Seven approved mAbs possessing various isoelectric point (pI) and hydrophobicity as well as a cysteine conjugated ADC were used in this study...
June 15, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28646653/cryo-em-beyond-the-microscope
#4
REVIEW
Lesley A Earl, Veronica Falconieri, Jacqueline Ls Milne, Sriram Subramaniam
The pace at which cryo-EM is being adopted as a mainstream tool in structural biology has continued unabated over the past year. Initial successes in obtaining near-atomic resolution structures with cryo-EM were enabled to a large extent by advances in microscope and detector technology. Here, we review some of the complementary technical improvements that are helping sustain the cryo-EM revolution. We highlight advances in image processing that permit high resolution structure determination even in the presence of structural and conformational heterogeneity...
June 21, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28646593/mri-assisted-dual-motion-correction-for-myocardial-perfusion-defect-detection-in-pet-imaging
#5
Xinhui Wang, Arman Rahmim, Jing Tang
PURPOSE: Myocardial perfusion (MP) PET imaging is a powerful tool in risk assess-assessment and stratification of patients with coronary artery disease. Involuntary organmotion degrades cardiac PET image resolution while respiratory and/or cardiac gating to freeze the motion leads to noisier reconstructed images due to reduced counts in the gated frames. In this work, we propose an MRI assisted dual motion correction method to compensate for respiratory and cardiac motion in MP PET data and study the impact of dual motion correction on MP defect detection using systematically designed experiments...
June 24, 2017: Medical Physics
https://www.readbyqxmd.com/read/28646351/rare-cyclophosphamide-induced-hemorrhagic-cystitis-in-a-chinese-population-with-rheumatic-diseases
#6
Liuqin Liang, Donging Chen, Xiaodong Wang, Zheng Yang, Jun Zhou, Zhongping Zhan, Fan Lian
OBJECTIVE: The aim of this study was to investigate the prevalence, severity, risk factors, and treatment outcomes of cyclophosphamide (CYC)-induced hemorrhagic cystitis (HC) in patients with rheumatic diseases. METHODS: We collected the clinical data from 1284 consecutive patients admitted to The First Affiliated Hospital of Sun Yat-Sen University who were treated with CYC between 2006 and 2016, and then conducted a retrospective analysis. RESULTS: The mean cumulative dose of CYC was 18...
June 23, 2017: Drugs—Real World Outcomes
https://www.readbyqxmd.com/read/28646301/random-initialisation-of-the-spectral-variables-an-alternate-approach-for-initiating-multivariate-curve-resolution-alternating-least-square-mcr-als-analysis
#7
Keshav Kumar
Multivariate curve resolution alternating least square (MCR-ALS) analysis is the most commonly used curve resolution technique. The MCR-ALS model is fitted using the alternate least square (ALS) algorithm that needs initialisation of either contribution profiles or spectral profiles of each of the factor. The contribution profiles can be initialised using the evolve factor analysis; however, in principle, this approach requires that data must belong to the sequential process. The initialisation of the spectral profiles are usually carried out using the pure variable approach such as SIMPLISMA algorithm, this approach demands that each factor must have the pure variables in the data sets...
June 23, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28646300/methodology-for-non-target-screening-of-sewage-sludge-using-comprehensive-two-dimensional-gas-chromatography-coupled-to-high-resolution-mass-spectrometry
#8
Cathrin Veenaas, Peter Haglund
To investigate the wide range of pollutants occurring in sewage sludge, an analytical method for comprehensive non-target screening is needed. To the best of our knowledge, no procedures currently exist for the full screening of organic contaminants in sewage sludge, which is the ultimate goal of this project. We developed non-discriminating sample preparation methods for gas chromatography-mass spectrometry (GC-MS) analysis. Pressurized liquid extraction (PLE) was used for extraction, with in-line (silica gel selective PLE, SPLE) or off-line clean-up (gel permeation chromatography, GPC)...
June 23, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28646299/effective-combined-water-and-sideband-suppression-for-low-speed-tissue-and-in-vivo-mas-nmr
#9
Yalda Liaghati Mobarhan, Jochem Struppe, Blythe Fortier-McGill, André J Simpson
High-resolution magic angle spinning (HR-MAS) NMR is a powerful technique that can provide metabolic profiles and structural constraints on intact biological and environmental samples such as cells, tissues and living organisms. However, centripetal force from fast spinning can lead to a loss of sample integrity. In analyses focusing on structural organization, metabolite compartmentalization or in vivo studies, it is critical to keep the sample intact. As such, there is growing interest in slow spinning studies that preserve sample longevity...
June 23, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28646298/an-on-off-on-fluorescent-probe-for-ascorbic-acid-based-on-cu-zncds-quantum-dots-and-%C3%AE-mno2-nanorods
#10
Hanbing Rao, Yao Gao, Hongwei Ge, Zhaoyi Zhang, Xin Liu, Yan Yang, Yaqin Liu, Wei Liu, Ping Zou, Yanying Wang, Xianxiang Wang, Hua He, Xianying Zeng
This work established a fluorescence approach for detecting ascorbic acid (AA) based on Cu-ZnCdS quantum dots (Cu-ZnCdS QDs) and α-MnO2 nanorods. Cu-ZnCdS QDs and α-MnO2 nanorods were characterized by high-resolution transmission electron microscopy (HRTEM), fluorescence spectroscopy, inductively coupled plasma optical emission spectroscopy (ICP-OES) and X-ray diffraction (XRD). In the presence of α-MnO2 nanorods, the fluorescence of Cu-ZnCdS QDs was greatly quenched through the inner filter effect (IFE)...
June 23, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28646214/structural-and-biochemical-studies-of-sulphotransferase-18-from-arabidopsis-thaliana-explain-its-substrate-specificity-and-reaction-mechanism
#11
Felix Hirschmann, Florian Krause, Petra Baruch, Igor Chizhov, Jonathan Wolf Mueller, Dietmar J Manstein, Jutta Papenbrock, Roman Fedorov
Sulphotransferases are a diverse group of enzymes catalysing the transfer of a sulfuryl group from 3'-phosphoadenosine 5'-phosphosulphate (PAPS) to a broad range of secondary metabolites. They exist in all kingdoms of life. In Arabidopsis thaliana (L.) Heynh. twenty-two sulphotransferase (SOT) isoforms were identified. Three of those are involved in glucosinolate (Gl) biosynthesis, glycosylated sulphur-containing aldoximes containing chemically different side chains, whose break-down products are involved in stress response against herbivores, pathogens, and abiotic stress...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646212/direct-observation-of-a-non-crystalline-state-of-li2s-p2s5-solid-electrolytes
#12
Hirofumi Tsukasaki, Shigeo Mori, Hideyuki Morimoto, Akitoshi Hayashi, Masahiro Tatsumisago
There are two types of solid electrolytes which has been recently expected to be applied to all-solid-state batteries. One is the glasses characterized by an amorphous state. The other is the glass ceramics containing crystalline in an amorphous matrix. However, the non-crystalline state of glasses and glass ceramics is still an open question. It has been anticipated that sea-island and core-shell structures including crystalline nanoparticles have been proposed as candidate models for glass ceramics. Nevertheless, no direct observation has been conducted so far...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646198/spatiotemporal-dynamics-of-grassland-aboveground-biomass-on-the-qinghai-tibet-plateau-based-on-validated-modis-ndvi
#13
Shiliang Liu, Fangyan Cheng, Shikui Dong, Haidi Zhao, Xiaoyun Hou, Xue Wu
Spatiotemporal dynamics of aboveground biomass (AGB) is a fundamental problem for grassland environmental management on the Qinghai-Tibet Plateau (QTP). Moderate Resolution Imaging Spectroradiometer (MODIS) Normalized Difference Vegetation Index (NDVI) data can feasibly be used to estimate AGB at large scales, and their precise validation is necessary to utilize them effectively. In our study, the clip-harvest method was used at 64 plots in QTP grasslands to obtain actual AGB values, and a handheld hyperspectral spectrometer was used to calculate field-measured NDVI to validate MODIS NDVI...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646193/fabrication-of-nitrogen-doped-nano-onions-and-their-electrocatalytic-activity-toward-the-oxygen-reduction-reaction
#14
E Y Choi, C K Kim
Nitrogen-doped nano-onions (NNO) were prepared as electrocatalytic materials for the oxygen reduction reaction (ORR). The nano-onions (NO), spherical graphitic material particles, were prepared by pyrolysis of nanodiamonds (ND). Oxidized NO (ONO) was prepared from NO by a modified Hummers' method, and this was mixed with urea, followed by pyrolysis, resulting in the formation of NNO. The nitrogen content and molar ratio of nitrogen-containing groups in the NNOs were varied by controlling the oxygen content of ONO to explore the effect of nitrogen content on the ORR activity...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646173/transmembrane-interactions-of-full-length-mammalian-bitopic-cytochrome-p450-cytochrome-b5-complex-in-lipid-bilayers-revealed-by-sensitivity-enhanced-dynamic-nuclear-polarization-solid-state-nmr-spectroscopy
#15
Kazutoshi Yamamoto, Marc A Caporini, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy
The dynamic protein-protein and protein-ligand interactions of integral bitopic membrane proteins with a single membrane-spanning helix play a plethora of vital roles in the cellular processes associated with human health and diseases, including signaling and enzymatic catalysis. While an increasing number of high-resolution structural studies of membrane proteins have successfully manifested an in-depth understanding of their biological functions, intact membrane-bound bitopic protein-protein complexes pose tremendous challenges for structural studies by crystallography or solution NMR spectroscopy...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646155/breast-cancer-multi-classification-from-histopathological-images-with-structured-deep-learning-model
#16
Zhongyi Han, Benzheng Wei, Yuanjie Zheng, Yilong Yin, Kejian Li, Shuo Li
Automated breast cancer multi-classification from histopathological images plays a key role in computer-aided breast cancer diagnosis or prognosis. Breast cancer multi-classification is to identify subordinate classes of breast cancer (Ductal carcinoma, Fibroadenoma, Lobular carcinoma, etc.). However, breast cancer multi-classification from histopathological images faces two main challenges from: (1) the great difficulties in breast cancer multi-classification methods contrasting with the classification of binary classes (benign and malignant), and (2) the subtle differences in multiple classes due to the broad variability of high-resolution image appearances, high coherency of cancerous cells, and extensive inhomogeneity of color distribution...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646146/optical-coherence-microscopy-as-a-novel-non-invasive-method-for-the-4d-live-imaging-of-early-mammalian-embryos
#17
Karol Karnowski, Anna Ajduk, Bartosz Wieloch, Szymon Tamborski, Krzysztof Krawiec, Maciej Wojtkowski, Maciej Szkulmowski
Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers' ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646144/using-fractional-intensities-of-time-resolved-fluorescence-to-sensitively-quantify-nadh-nad-with-genetically-encoded-fluorescent-biosensors
#18
Mengfang Chang, Lei Li, Hanyang Hu, Qingxun Hu, Aoxue Wang, Xiaodan Cao, Xiantong Yu, Sanjun Zhang, Yuzheng Zhao, Jinquan Chen, Yi Yang, Jianhua Xu
In this paper, we propose a novel and sensitive ratiometric analysis method that uses the fractional intensities of time-resolved fluorescence of genetically encoded fluorescent NADH/NAD(+) biosensors, Peredox, SoNar, and Frex. When the conformations of the biosensors change upon NADH/NAD(+) binding, the fractional intensities (α i τ i ) have opposite changing trends. Their ratios could be exploited to quantify NADH/NAD(+) levels with a larger dynamic range and higher resolution versus commonly used fluorescence intensity and lifetime methods...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646132/global-rainfall-erosivity-assessment-based-on-high-temporal-resolution-rainfall-records
#19
Panos Panagos, Pasquale Borrelli, Katrin Meusburger, Bofu Yu, Andreas Klik, Kyoung Jae Lim, Jae E Yang, Jinren Ni, Chiyuan Miao, Nabansu Chattopadhyay, Seyed Hamidreza Sadeghi, Zeinab Hazbavi, Mohsen Zabihi, Gennady A Larionov, Sergey F Krasnov, Andrey V Gorobets, Yoav Levi, Gunay Erpul, Christian Birkel, Natalia Hoyos, Victoria Naipal, Paulo Tarso S Oliveira, Carlos A Bonilla, Mohamed Meddi, Werner Nel, Hassan Al Dashti, Martino Boni, Nazzareno Diodato, Kristof Van Oost, Mark Nearing, Cristiano Ballabio
The exposure of the Earth's surface to the energetic input of rainfall is one of the key factors controlling water erosion. While water erosion is identified as the most serious cause of soil degradation globally, global patterns of rainfall erosivity remain poorly quantified and estimates have large uncertainties. This hampers the implementation of effective soil degradation mitigation and restoration strategies. Quantifying rainfall erosivity is challenging as it requires high temporal resolution(<30 min) and high fidelity rainfall recordings...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646078/stereospecific-metabolism-of-r-and-s-warfarin-by-human-hepatic-cytosolic-reductases
#20
Dustyn A Barnette, Bryce Johnson, Dakota L Pouncey, Robert Nshimiyimana, Linda Desrochers, Thomas E Goodwin, Grover Paul Miller
Coumadin (rac-warfarin) is the most commonly used anticoagulant in the world, yet its clinical use is often challenging due to a narrow therapeutic range and inter-individual variations in response. A critical contributor to the uncertainty is variability in warfarin metabolism, which includes mostly oxidative but also reductive pathways. Reduction of each warfarin enantiomer yields two warfarin alcohol isomers, and the corresponding four alcohols retain varying levels of anticoagulant activity. Studies on the kinetics of warfarin reduction have often lacked resolution of parent drug enantiomers and suffered from co-elution of pairs of alcohol metabolites; thus, those studies fail to establish the importance of individual, stereospecific reductive pathways...
June 23, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
keyword
keyword
12826
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"