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https://www.readbyqxmd.com/read/28205268/the-effect-of-delta-like-1-homologue-on-the-proliferation-and-odontoblastic-differentiation-in-human-dental-pulp-stem-cells
#1
Shengcai Qi, Yanhong Yan, Yue Wen, Jialiang Li, Jing Wang, Fubo Chen, Xiaoshan Tang, Guangwei Shang, Yuanzhi Xu, Raorao Wang
INTRODUCTION: This study aimed to investigate the functions of delta-like homologue 1 (DLK1) in the proliferation and differentiation of human dental pulp stem cells (hDPSCs). METHODS: Immunohistochemical analysis was used to determine the expression of alkaline phosphatase (ALP), dentin sialophosphoprotein (DSPP), DLK1, NOTCH1 and p-ERK1/2 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs. The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay respectively...
February 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28174625/sex-and-age-differences-in-the-expression-of-liver-micrornas-during-the-life-span-of-f344-rats
#2
Joshua C Kwekel, Vikrant Vijay, Tao Han, Carrie L Moland, Varsha G Desai, James C Fuscoe
BACKGROUND: Physiological factors such as age and sex have been shown to be risk factors for adverse effects in the liver, including liver diseases and drug-induced liver injury. Previously, we have reported age- and sex-related significant differences in hepatic basal gene expression in rats during the life span that may be related to susceptibility to such adverse effects. However, the underlying mechanisms of the gene expression changes were not fully understood. In recent years, increasing evidence for epigenetic mechanisms of gene regulation has fueled interest in the role of microRNAs (miRNAs) in toxicogenomics and biomarker discovery...
2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28110695/men1-and-micrornas-the-link-between-sporadic-pituitary-parathyroid-and-adrenocortical-tumors
#3
Z Nagy, P M Szabó, V K Grolmusz, P Perge, I Igaz, A Patócs, P Igaz
Sporadic tumors of the pituitary, parathyroids and adrenal cortex are unique, as their benign forms are very common, but malignant forms are exceptionally rare. Hereditary forms of these tumors occur in multiple endocrine neoplasia syndrome type 1 (MEN1). We hypothesize that the pathogenic link among the sporadic tumors of these organs of different germ layers might be represented by common molecular pathways involving the MEN1 gene and microRNAs (miR). miR-24 might be a microRNA linking the three tumor entities, but other candidates such as miR-142-3p and microRNAs forming the DLK1-MEG3 miRNA cluster might also be of importance...
February 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28099888/the-non-canonical-notch1-ligand-delta-like-1-homolog-dlk1-self-interacts-in-mammals
#4
Gunnhildur Ásta Traustadóttir, Charlotte Harken Jensen, Jose Javier Garcia Ramirez, Hans Christian Beck, Søren Paludan Sheikh, Ditte Caroline Andersen
Delta-like 1 homolog (DLK1) is an imprinted gene, which is widely expressed during mammalian development and plays a pivotal role in differentiation of various tissue types. Most recently, we have shown that DLK1 interacts with NOTCH1, yet several Notch independent mechanisms have previously been suggested as well, but only poorly confirmed in a mammalian context. In the present study, we employed the mammalian two-hybrid (MTH) system, a genetic in vivo protein-protein interaction system, to show robust DLK1-DLK1, DLK1-FnI (Fibronectin) and DLK1-CFR (cysteine-rich FGF receptor) interactions, whereas the proposed DLK1-IGFBP1 interaction was not supported by MTH...
April 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28043921/microrna-134-3p-is-a-novel-potential-inhibitor-of-human-ovarian-cancer-stem-cells-by-targeting-rab27a
#5
Cui Chang, Te Liu, Yongyi Huang, Wenxing Qin, Hongtu Yang, Juan Chen
The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44-/CD133- ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3'-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes...
March 20, 2017: Gene
https://www.readbyqxmd.com/read/27992376/myostatin-deficiency-in-mice-increases-global-gene-expression-at-the-dlk1-dio3-locus-in-the-skeletal-muscle
#6
Keisuke Hitachi, Kunihiro Tsuchida
Myostatin, a member of the transforming growth factor-beta superfamily, is a negative regulator of skeletal muscle growth and development. Myostatin inhibition leads to increased skeletal muscle mass in mammals; hence, myostatin is considered a potential therapeutic target for skeletal muscle wasting. However, downstream molecules of myostatin in the skeletal muscle have not been fully elucidated. Here, we identified the Dlk1-Dio3 locus at the mouse chromosome 12qF1, also called as the callipyge locus in sheep, as a novel downstream target of myostatin...
January 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/27925264/three-intronic-lncrnas-with-monoallelic-expression-derived-from-the-meg8-gene-in-cattle
#7
W Yang, D Li, G Wang, C Zhang, M Zhang, W Zhang, S Li
The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years. Antisense lncRNAs, intergenetic lncRNAs and enhancer lncRNAs can regulate genomic imprinting, which leads to parent-origin-specific monoalletic expression of genes. However, the function of intronic ncRNAs in genomic imprinting remains unclear. Previously, we obtained the cDNA sequence of cattle MEG8 gene, which is located in the DLK1-DIO3 imprinted clusters of cattle chromosome 21. In this study, we undertook a systematic search for transcripts mapping to the MEG8 intronic region and identified three novel lncRNAs, named MEG8 intronic RNA 1 (MEG8-IT1), MEG8 intronic RNA 2 (MEG8-IT2) and MEG8 intronic RNA 3 (MEG8-IT3) according to the GENCODE annotated bibliography...
December 7, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27904782/prognostic-values-of-dlk1-for-surgery-and-imatinib-mesylate-adjuvant-therapy-in-gastrointestinal-stromal-tumors
#8
Jia Xu, Ming Wang, Zizhen Zhang, Wenyi Zhao, Chaojie Wang, Lin Tu, Yeqian Zhang, Hui Cao
The Delta-like 1 homolog (DLK1) gene is a paternal imprinting gene located on human chromosome 14q32, a site associated with frequent chromosomal mutations in GIST. The expression level of DLK1 is closely associated with the outcome of tumours. However, no study has reported the DLK1 expression in GIST. Here, we demonstrated that DLK1 showed low expression in GIST patients with low risk according to the modified National Institute of Health (NIH) criteria. With increasing tumour risk level, DLK1 gene and protein expression levels gradually increased...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27904015/deletion-of-conserved-sequences-in-ig-dmr-at-dlk1-gtl2-locus-suggests-their-involvement-in-expression-of-paternally-expressed-genes-in-mice
#9
Takeshi Saito, Satoshi Hara, Moe Tamano, Hiroshi Asahara, Shuji Takada
Expression regulation of the Dlk1-Dio3 imprinted domain by the intergenic differentially methylated region (IG-DMR) is essential for normal embryonic development in mammals. In this study, we investigated conserved IG-DMR genomic sequences in eutherians to elucidate their role in genomic imprinting of the Dlk1-Dio3 domain. Using a comparative genomics approach, we identified three highly conserved sequences in IG-DMR. To elucidate the functions of these sequences in vivo, we generated mutant mice lacking each of the identified highly conserved sequences using the CRISPR/Cas9 system...
February 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27899651/a-permissive-chromatin-state-regulated-by-zfp281-aff3-in-controlling-the-imprinted-meg3-polycistron
#10
Yan Wang, Yang Shen, Qian Dai, Qian Yang, Yue Zhang, Xin Wang, Wei Xie, Zhuojuan Luo, Chengqi Lin
Genomic imprinting is an epigenetic regulation that leads to gene expression in a parent-of-origin specific manner. AFF3, the central component of the Super Elongation Complex-like 3 (SEC-L3), is enriched at both the intergenic-differentially methylated region (IG-DMR) and the Meg3 enhancer within the imprinted Dlk1-Dio3 locus to regulate the allele-specific gene expression in this locus. The localization of AFF3 to IG-DMR requires ZFP57. However, how AFF3 functions at the Meg3 enhancer in maintaining allele-specific gene expression remains unclear...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27858207/an-imprinted-long-noncoding-rna-located-between-genes-meg8-and-meg9-in-the-cattle-dlk1-dio3-domain
#11
Mingyue Zhang, Yupeng Zhao, Guannan Wang, Dongjie Li, Weina Chen, Cui Zhang, Shijie Li
The Dlk1-Dio3 imprinted domain is located on the cattle chromosome 21 and contains three paternally expressed protein-coding genes and a number of maternally expressed short or long noncoding RNA genes. We have previously obtained two maternally expressed long noncoding RNA genes, Meg8 and Meg9, from the cattle. In this study, we identified a novel noncoding RNA located between Meg8 and Meg9 known as LINC24061 according to the GENCODE annotated bibliography. Two alternatively spliced transcripts (LINC24061-v1 and LINC24061-v2) were obtained using RT-PCR and RACE, and the expression pattern of LINC24061-v1 and LINC24061-v2 was shown to be tissue-specific...
November 17, 2016: Genetica
https://www.readbyqxmd.com/read/27857161/gene-expression-profiling-of-breast-cancer-in-lebanese-women
#12
Joelle Makoukji, Nadine J Makhoul, Maya Khalil, Sally El-Sitt, Ehab Saad Aldin, Mark Jabbour, Fouad Boulos, Emanuela Gadaleta, Ajanthah Sangaralingam, Claude Chelala, Rose-Mary Boustany, Arafat Tfayli
Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2...
November 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27848971/epigenetic-and-genetic-components-of-height-regulation
#13
Stefania Benonisdottir, Asmundur Oddsson, Agnar Helgason, Ragnar P Kristjansson, Gardar Sveinbjornsson, Arna Oskarsdottir, Gudmar Thorleifsson, Olafur B Davidsson, Gudny A Arnadottir, Gerald Sulem, Brynjar O Jensson, Hilma Holm, Kristjan F Alexandersson, Laufey Tryggvadottir, G Bragi Walters, Sigurjon A Gudjonsson, Lucas D Ward, Jon K Sigurdsson, Paul D Iordache, Michael L Frigge, Thorunn Rafnar, Augustine Kong, Gisli Masson, Hannes Helgason, Unnur Thorsteinsdottir, Daniel F Gudbjartsson, Patrick Sulem, Kari Stefansson
Adult height is a highly heritable trait. Here we identified 31.6 million sequence variants by whole-genome sequencing of 8,453 Icelanders and tested them for association with adult height by imputing them into 88,835 Icelanders. Here we discovered 13 novel height associations by testing four different models including parent-of-origin (|β|=0.4-10.6 cm). The minor alleles of three parent-of-origin signals associate with less height only when inherited from the father and are located within imprinted regions (IGF2-H19 and DLK1-MEG3)...
November 16, 2016: Nature Communications
https://www.readbyqxmd.com/read/27829231/deregulation-of-small-non-coding-rnas-at-the-dlk1-dio3-imprinted-locus-predicts-lung-cancer-patient-outcome
#14
Katey S S Enfield, Victor D Martinez, Erin A Marshall, Greg L Stewart, Sonia H Y Kung, Jhon R Enterina, Wan L Lam
Deregulation of the imprinted DLK1-DIO3 locus at chromosome 14q32.1-14q32.31 has been associated with developmental and respiratory disorders, including cancer. In lung cancer, deregulation of imprinting at DLK1-DIO3 was recently described in smokers. Deregulated expression of a microRNA (miRNA) cluster mapping to this locus was also associated with patient outcome, suggesting the importance of this locus to lung cancer disease phenotypes. The DLK1-DIO3 locus is complex, and encodes several protein-coding genes, in addition to long and short non-coding RNAs...
November 5, 2016: Oncotarget
https://www.readbyqxmd.com/read/27811939/metabolism-dlk1-levels-predict-fetal-growth-restriction
#15
Tim Geach
No abstract text is available yet for this article.
January 2017: Nature Reviews. Endocrinology
https://www.readbyqxmd.com/read/27798673/identification-and-expression-profiling-of-mirnaome-in-goat-longissimus-dorsi-muscle-from-prenatal-stages-to-a-neonatal-stage
#16
Jiazhong Guo, Wei Zhao, Siyuan Zhan, Li Li, Tao Zhong, Linjie Wang, Yao Dong, Hongping Zhang
Skeletal muscle development is a complex biological process regulated by numerous genes and non-coding RNAs, such as microRNAs (miRNAs). In the current study, we made use of the deep sequencing data from Jianzhou Da'er goat longissimus dorsi sampled on days 45, 60, and 105 of gestation, as well as day three after birth to identify miRNAs that regulate goat skeletal myogenesis, and examine their temporal expression profiles. A total of 410 known goat miRNAs, 752 miRNA homologs and 88 novel miRNAs were identified across four stages...
2016: PloS One
https://www.readbyqxmd.com/read/27785886/population-genomics-fits-the-bill-genetics-of-adaptive-beak-variation-in-darwin-s-finches
#17
Nicholas I Mundy
Darwin's finches are an iconic case of adaptive radiation. The size and shape of their beaks are key adaptive traits related to trophic niche that vary among species and evolve rapidly when the food supply changes. Building on recent studies, a paper in this issue of Molecular Ecology (Chaves et al. ) investigates the genomic basis of beak size variation in sympatric populations of three species of ground finch (Geospiza) by performing a Genome-wide association study using RAD-seq data. The authors find that variation in a small number of markers can explain a substantial proportion of variation in beak size...
November 2016: Molecular Ecology
https://www.readbyqxmd.com/read/27777636/maternal-vitamin-d-depletion-alters-dna-methylation-at-imprinted-loci-in-multiple-generations
#18
Jing Xue, Sarah A Schoenrock, William Valdar, Lisa M Tarantino, Folami Y Ideraabdullah
BACKGROUND: Environmental perturbation of epigenetic mechanisms is linked to a growing number of diseases. Characterizing the role environmental factors play in modifying the epigenome is important for disease etiology. Vitamin D is an essential nutrient affecting brain, bone, heart, immune and reproductive health. Vitamin D insufficiency is a global issue, and the role in maternal and child health remains under investigation. METHODS: We used Collaborative Cross (CC) inbred mice to characterize the effect of maternal vitamin D depletion on offspring phenotypic and epigenetic outcomes at imprinted domains (H19/Igf2, Snrpn, Dlk1/Gtl2, and Grb10) in the soma (liver) and germline (sperm)...
2016: Clinical Epigenetics
https://www.readbyqxmd.com/read/27777362/associations-between-fetal-imprinted-genes-and-maternal-blood-pressure-in-pregnancy
#19
Clive J Petry, Nuria Sanz Marcos, Gracielle Pimentel, M Geoffrey Hayes, Michael Nodzenski, Denise M Scholtens, Ieuan A Hughes, Carlo L Acerini, Ken K Ong, William L Lowe, David B Dunger
In addition to maternal genes and environmental exposures, variation in fetal imprinted genes could also affect maternal blood pressure during pregnancy. Our objective was to test the associations between polymorphic variants in 16 imprinted genes and maternal mean arterial blood pressures in 1160 DNA trios from 2 established birth cohorts (the Cambridge Baby Growth and Wellbeing Studies) and seek replication in 1367 Hyperglycemia and Adverse Pregnancy Outcome Study participants. Significant univariate associations, all independent of fetal sex, were observed in the Cambridge cohorts, including FAM99A rs1489945 transmitted from the mother (P=2×10(-4)), DLK1 rs10139403 (mother; P=9×10(-4)), DLK1 rs12147008 (mother; P=1×10(-3)), H19 rs217222 (father; P=1×10(-3)), SNRPN rs1453556 (father; P=1×10(-3)), IGF2 rs6356 (father; P=1×10(-3)), and NNAT rs6066671 (father; P=1×10(-3))...
December 2016: Hypertension
https://www.readbyqxmd.com/read/27776119/fetus-derived-dlk1-is-required-for-maternal-metabolic-adaptations-to-pregnancy-and-is-associated-with-fetal-growth-restriction
#20
Mary A M Cleaton, Claire L Dent, Mark Howard, Jennifer A Corish, Isabelle Gutteridge, Ulla Sovio, Francesca Gaccioli, Nozomi Takahashi, Steven R Bauer, D Steven Charnock-Jones, Theresa L Powell, Gordon C S Smith, Anne C Ferguson-Smith, Marika Charalambous
Pregnancy is a state of high metabolic demand. Fasting diverts metabolism to fatty acid oxidation, and the fasted response occurs much more rapidly in pregnant women than in non-pregnant women. The product of the imprinted DLK1 gene (delta-like homolog 1) is an endocrine signaling molecule that reaches a high concentration in the maternal circulation during late pregnancy. By using mouse models with deleted Dlk1, we show that the fetus is the source of maternal circulating DLK1. In the absence of fetally derived DLK1, the maternal fasting response is impaired...
December 2016: Nature Genetics
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