keyword
MENU ▼
Read by QxMD icon Read
search

MELK

keyword
https://www.readbyqxmd.com/read/27920471/identification-of-il11ra-and-melk-amplification-in-gastric-cancer-by-comprehensive-genomic-profiling-of-gastric-cancer-cell-lines
#1
Danielle Queiroz Calcagno, Sylvia Santomi Takeno, Carolina Oliveira Gigek, Mariana Ferreira Leal, Fernanda Wisnieski, Elizabeth Suchi Chen, Taíssa Maíra Thomaz Araújo, Eleonidas Moura Lima, Maria Isabel Melaragno, Samia Demachki, Paulo Pimentel Assumpção, Rommel Rodriguez Burbano, Marília Cardoso Smith
AIM: To identify common copy number alterations on gastric cancer cell lines. METHODS: Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS: The amplification of 9p13...
November 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27915972/resistance-to-cell-death-and-its-modulation-in-cancer-stem-cells
#2
Ahmad R Safa
Accumulating evidence has demonstrated that human cancers arise from various tissues of origin that initiate from cancer stem cells (CSCs) or cancer-initiating cells. The extrinsic and intrinsic apoptotic pathways are dysregulated in CSCs, and these cells play crucial roles in tumor initiation, progression, cell death resistance, chemo- and radiotherapy resistance, and tumor recurrence. Understanding CSC-specific signaling proteins and pathways is necessary to identify specific therapeutic targets that may lead to the development of more efficient therapies selectively targeting CSCs...
2016: Critical Reviews in Oncogenesis
https://www.readbyqxmd.com/read/27867630/melk-kinase-holds-promise-as-a-new-radiosensitizing-target-and-biomarker-in-triple-negative-breast-cancer
#3
COMMENT
Carlos S Moreno
No abstract text is available yet for this article.
October 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27827310/cardiovascular-phenotypes-in-children-with-ckd-the-4c-study
#4
Franz Schaefer, Anke Doyon, Karolis Azukaitis, Aysun Bayazit, Nur Canpolat, Ali Duzova, Ana Niemirska, Betul Sözeri, Daniela Thurn, Ali Anarat, Bruno Ranchin, Mieczyslav Litwin, Salim Caliskan, Cengiz Candan, Esra Baskin, Ebru Yilmaz, Sevgi Mir, Marietta Kirchner, Anja Sander, Dieter Haffner, Anette Melk, Elke Wühl, Rukshana Shroff, Uwe Querfeld
BACKGROUND AND OBJECTIVES: Cardiovascular disease is the most important comorbidity affecting long-term survival in children with CKD. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: The Cardiovascular Comorbidity in Children with CKD Study is a multicenter, prospective, observational study in children ages 6-17 years old with initial GFR of 10-60 ml/min per 1.73 m(2). The cardiovascular status is monitored annually, and subclinical cardiovascular disease is assessed by noninvasive measurements of surrogate markers, including the left ventricular mass index, carotid intima-media thickness, and central pulse wave velocity...
November 8, 2016: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/27798878/expression-of-maternal-embryonic-leucine-zipper-kinase-melk-correlates-to-malignant-potentials-in-hepatocellular-carcinoma
#5
Kiyokazu Hiwatashi, Shinichi Ueno, Masahiko Sakoda, Satoshi Iino, Koji Minami, Keiichi Yonemori, Yuka Nishizono, Hiroshi Kurahara, Yuko Mataki, Kosei Maemura, Hiroyuki Shinchi, Shoji Natsugoe
BACKGROUND/AIM: Maternal embryonic leucine zipper kinase (MELK) is categorized as a member of AMP-activated protein kinase families. Various MELK-associated cellular and biological processes affect multiple stages of tumorigenesis. The aim of the present study was to clarify the relationship between MELK expression and hepatocellular carcinoma (HCC) clinicopathological features. MATERIALS AND METHODS: In thirty conserved frozen primary HCC and non-HCC samples MELK mRNA expression was examined by quantitative real-time polymerase chain reaction (PCR)...
October 2016: Anticancer Research
https://www.readbyqxmd.com/read/27715411/the-impact-of-autophagy-on-the-development-of-senescence-in-primary-tubular-epithelial-cells
#6
Arpita Baisantry, Sagar Bhayana, Christoph Wrede, Jan Hegermann, Hermann Haller, Anette Melk, Roland Schmitt
Autophagy and senescence are 2 distinct pathways that are importantly involved in acute kidney injury and renal repair. Recent data indicate that the 2 processes might be interrelated. To investigate the potential link between autophagy and senescence in the kidney we isolated primary tubular epithelial cells (PTEC) from wild-type mice and monitored the occurrence of cellular senescence during autophagy activation and inhibition. We found that the process of cell isolation and transfer into culture was associated with a strong basal autophagic activation in PTEC...
November 2016: Cell Cycle
https://www.readbyqxmd.com/read/27693640/melk-is-an-oncogenic-kinase-essential-for-early-hepatocellular-carcinoma-recurrence
#7
Hongping Xia, Shik Nie Kong, Jianxiang Chen, Ming Shi, Karthik Sekar, Veerabrahma Pratap Seshachalam, Muthukumar Rajasekaran, Brian Kim Poh Goh, London Lucien Ooi, Kam M Hui
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. Many kinases have been found to be intimately involved in oncogenesis and the deregulation of kinase function has emerged as a major mechanism by which cancer cells evade normal physiological constraints on growth and survival. Previously, we have performed gene expression profile analysis on HCC samples and have identified a host of kinases that are remarkably overexpressed in HCC. Among these, the Maternal Embryonic Leucine Zipper Kinase (MELK) is highly overexpressed in HCC and its overexpression strongly correlates with early recurrence and poor patients' survival...
September 28, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27600870/biased-%C3%AE-opioid-receptor-agonists-diversely-regulate-lateral-mobility-and-functional-coupling-of-the-receptor-to-its-cognate-g-proteins
#8
Barbora Melkes, Lucie Hejnova, Jiri Novotny
There are some indications that biased μ-opioid ligands may diversely affect μ-opioid receptor (MOR) properties. Here, we used confocal fluorescence recovery after photobleaching (FRAP) to study the regulation by different MOR agonists of receptor movement within the plasma membrane of HEK293 cells stably expressing a functional yellow fluorescent protein (YFP)-tagged μ-opioid receptor (MOR-YFP). We found that the lateral mobility of MOR-YFP was increased by (D-Ala(2),N-MePhe(4),Gly(5)-ol)-enkephalin (DAMGO) and to a lesser extent also by morphine but decreased by endomorphin-2...
September 6, 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/27574806/kinase-gene-expression-profiling-of-metastatic-clear-cell-renal-cell-carcinoma-tissue-identifies-potential-new-therapeutic-targets
#9
Pooja Ghatalia, Eddy S Yang, Brittany N Lasseigne, Ryne C Ramaker, Sara J Cooper, Dongquan Chen, Sunil Sudarshan, Shi Wei, Arjun S Guru, Amy Zhao, Tiffiny Cooper, Deborah L Della Manna, Gurudatta Naik, Richard M Myers, Guru Sonpavde
Kinases are therapeutically actionable targets. Kinase inhibitors targeting vascular endothelial growth factor receptors (VEGFR) and mammalian target of rapamycin (mTOR) improve outcomes in metastatic clear cell renal cell carcinoma (ccRCC), but are not curative. Metastatic tumor tissue has not been comprehensively studied for kinase gene expression. Paired intra-patient kinase gene expression analysis in primary tumor (T), matched normal kidney (N) and metastatic tumor tissue (M) may assist in identifying drivers of metastasis and prioritizing therapeutic targets...
2016: PloS One
https://www.readbyqxmd.com/read/27540718/anti-myeloma-activity-of-melk-inhibitor-ots167-effects-on-drug-resistant-myeloma-cells-and-putative-myeloma-stem-cell-replenishment-of-malignant-plasma-cells
#10
A T Stefka, J-H Park, Y Matsuo, S Chung, Y Nakamura, A J Jakubowiak, S Rosebeck
No abstract text is available yet for this article.
2016: Blood Cancer Journal
https://www.readbyqxmd.com/read/27528663/mitotic-melk-eif4b-signaling-controls-protein-synthesis-and-tumor-cell-survival
#11
Yubao Wang, Michael Begley, Qing Li, Hai-Tsang Huang, Ana Lako, Michael J Eck, Nathanael S Gray, Timothy J Mitchison, Lewis C Cantley, Jean J Zhao
The protein kinase maternal and embryonic leucine zipper kinase (MELK) is critical for mitotic progression of cancer cells; however, its mechanisms of action remain largely unknown. By combined approaches of immunoprecipitation/mass spectrometry and peptide library profiling, we identified the eukaryotic translation initiation factor 4B (eIF4B) as a MELK-interacting protein during mitosis and a bona fide substrate of MELK. MELK phosphorylates eIF4B at Ser406, a modification found to be most robust in the mitotic phase of the cell cycle...
August 30, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27508705/-op-4b-10-left-ventricular-mass-after-renal-transplantation-metaanalysis-of-cross-sectional-and-longitudinal-studies
#12
B Schmidt, K Bergmann, J Kaufeld, A Melk
OBJECTIVE: Left ventriuclar mass (LVM) is a strong predictor of cardiovascular events. In patients with chronic kidney disease and in patients on dialysis left ventricular hypertrophy is highly prevalent. Overcoming uremia by renal transplantation might improve LVM. However, after transplantation treatment with steroids and calcineurin inhibitors increases blood pressure and therefore might counteract the positive effect of restoring renal function.We performed a metaanalysis of the available evidence from observational studies in patientts after renal tranplantation...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27399530/332%C3%A2-molecular-characteristics-of-tumor-infiltrating-front-in-glioblastoma-insights-into-molecular-heterogeneity-and-implications-on-targeted-therapy
#13
Arivazhagan Arimappamagan, Kruthika, Rose Dawn, Kondaiah Paturu, Vani Santosh
INTRODUCTION: Molecular heterogeneity in glioblastoma (GBM) is a novel area of research recently. Because tumor infiltrating front in GBM is often left behind following tumor decompression, knowledge of its genetic makeup can improve the rationale of potential molecular targets. METHODS: MRI localized biopsies of the tumor core and peritumoral brain zone (PBZ) were obtained from 25 GBM patients. The PBZ samples containing 15% to 30% tumor cells and core samples with predominantly tumor cells along with 8 nonneoplastic brain tissues were selected for a whole Genome Gene Expression Microarray...
August 2016: Neurosurgery
https://www.readbyqxmd.com/read/27313313/genetic-environmental-and-disease-associated-correlates-of-vitamin-d-status-in-children-with-ckd
#14
Anke Doyon, Bettina Schmiedchen, Anja Sander, Aysun Bayazit, Ali Duzova, Nur Canpolat, Daniela Thurn, Karolis Azukaitis, Ali Anarat, Justine Bacchetta, Sevgi Mir, Rukshana Shroff, Ebru Yilmaz, Cengiz Candan, Markus Kemper, Michel Fischbach, Gerard Cortina, Günter Klaus, Matthias Wuttke, Anna Köttgen, Anette Melk, Uwe Querfeld, Franz Schaefer
BACKGROUND AND OBJECTIVES: Vitamin D deficiency is endemic in children with CKD. We sought to investigate the association of genetic disposition, environmental factors, vitamin D supplementation, and renal function on vitamin D status in children with CKD. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: Serum 25-hydroxy-vitamin D, 1,25-dihydroxy-vitamin D, and 24,25-dihydroxy-vitamin D concentrations were measured cross-sectionally in 500 children from 12 European countries with CKD stages 3-5...
July 7, 2016: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/27301292/connection-between-proliferation-rate-and-temozolomide-sensitivity-of-primary-glioblastoma-cell-culture-and-expression-of-yb-1-and-lrp-mvp
#15
N I Moiseeva, O Yu Susova, A A Mitrofanov, D Yu Panteleev, G V Pavlova, N A Pustogarov, A A Stavrovskaya, E Yu Rybalkina
Glioblastomas (GBL) are the most common and aggressive brain tumors. They are distinguished by high resistance to radiation and chemotherapy. To find novel approaches for GBL classification, we obtained 16 primary GBL cell cultures and tested them with real-time PCR for mRNA expression of several genes (YB-1, MGMT, MELK, MVP, MDR1, BCRP) involved in controlling cell proliferation and drug resistance. The primary GBL cultures differed in terms of proliferation rate, wherein a group of GBL cell cultures with low proliferation rate demonstrated higher resistance to temozolomide...
June 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27260389/problems-of-glioblastoma-multiforme-drug-resistance
#16
REVIEW
A A Stavrovskaya, S S Shushanov, E Yu Rybalkina
Glioblastoma multiforme (GBL) is the most common and aggressive brain neoplasm. A standard therapeutic approach for GBL involves combination therapy consisting of surgery, radiotherapy, and chemotherapy. The latter is based on temozolomide (TMZ). However, even by applying such a radical treatment strategy, the mean patient survival time is only 14.6 months. Here we review the molecular mechanisms underlying the resistance of GBL cells to TMZ including genetic and epigenetic mechanisms. Present data regarding a role for genes and proteins MGMT, IDH1/2, YB-1, MELK, MVP/LRP, MDR1 (ABCB1), and genes encoding other ABC transporters as well as Akt3 kinase in developing resistance of GBL to TMZ are discussed...
February 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27225691/maternal-embryonic-leucine-zipper-kinase-melk-as-a-novel-mediator-and-biomarker-of-radioresistance-in-human-breast-cancer
#17
Corey Speers, Shuang G Zhao, Vishal Kothari, Alyssa Santola, Meilan Liu, Kari Wilder-Romans, Joseph Evans, Nidhi Batra, Harry Bartelink, Daniel F Hayes, Theodore S Lawrence, Powel H Brown, Lori J Pierce, Felix Y Feng
PURPOSE: While effective targeted therapies exist for estrogen receptor-positive and HER2-positive breast cancer, no such effective therapies exist for triple-negative breast cancer (TNBC); thus, it is clear that additional targets for radiosensitization and treatment are critically needed. EXPERIMENTAL DESIGN: Expression microarrays, qRT-PCR, and Western blotting were used to assess MELK RNA and protein expression levels. Clonogenic survival assays were used to quantitate the radiosensitivity of cell lines at baseline and after MELK inhibition...
December 1, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27187609/toward-the-validation-of-maternal-embryonic-leucine-zipper-kinase-discovery-optimization-of-highly-potent-and-selective-inhibitors-and-preliminary-biology-insight
#18
B Barry Touré, John Giraldes, Troy Smith, Elizabeth R Sprague, Yaping Wang, Simon Mathieu, Zhuoliang Chen, Yuji Mishina, Yun Feng, Yan Yan-Neale, Subarna Shakya, Dongshu Chen, Matthew Meyer, David Puleo, J Tres Brazell, Christopher Straub, David Sage, Kirk Wright, Yanqiu Yuan, Xin Chen, Jose Duca, Sean Kim, Li Tian, Eric Martin, Kristen Hurov, Wenlin Shao
MELK kinase has been implicated in playing an important role in tumorigenesis. Our previous studies suggested that MELK is involved in the regulation of cell cycle and its genetic depletion leads to growth inhibition in a subset of high MELK-expressing basal-like breast cancer cell lines. Herein we describe the discovery and optimization of novel MELK inhibitors 8a and 8b that recapitulate the cellular effects observed by short hairpin ribonucleic acid (shRNA)-mediated MELK knockdown in cellular models. We also discovered a novel fluorine-induced hydrophobic collapse that locked the ligand in its bioactive conformation and led to a 20-fold gain in potency...
May 26, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27082996/otssp167-abrogates-mitotic-checkpoint-through-inhibiting-multiple-mitotic-kinases
#19
Wenbin Ji, Christopher Arnst, Aaron R Tipton, Michael E Bekier, William R Taylor, Tim J Yen, Song-Tao Liu
OTSSP167 was recently characterized as a potent inhibitor for maternal embryonic leucine zipper kinase (MELK) and is currently tested in Phase I clinical trials for solid tumors that have not responded to other treatment. Here we report that OTSSP167 abrogates the mitotic checkpoint at concentrations used to inhibit MELK. The abrogation is not recapitulated by RNAi mediated silencing of MELK in cells. Although OTSSP167 indeed inhibits MELK, it exhibits off-target activity against Aurora B kinase in vitro and in cells...
2016: PloS One
https://www.readbyqxmd.com/read/27022301/identification-and-prioritization-of-candidate-genes-for-symptom-variability-in-breast-cancer-survivors-based-on-disease-characteristics-at-the-cellular-level
#20
Theresa A Koleck, Yvette P Conley
Research is beginning to suggest that the presence and/or severity of symptoms reported by breast cancer survivors may be associated with disease-related factors of cancer. In this article, we present a novel approach to the identification and prioritization of biologically plausible candidate genes to investigate relationships between genomic variation and symptom variability in breast cancer survivors. Cognitive dysfunction is utilized as a representative breast cancer survivor symptom to elucidate the conceptualization of and justification for our cellular, disease-based approach to address symptom variability in cancer survivors...
2016: Breast Cancer: Targets and Therapy
keyword
keyword
12753
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"