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https://www.readbyqxmd.com/read/28433865/high-sensitivity-hplc-method-for-analysis-of-in-vivo-extracellular-gaba-using-optimized-fluorescence-parameters-for-o-phthalaldehyde-opa-sulfite-derivatives
#1
Shannon L Zandy, James M Doherty, Nathan D Wibisono, Rueben A Gonzales
Reversed-phase HPLC with derivatization using o-phthalaldehyde (OPA) and sulfite allows electrochemical detection of γ-aminobutyric acid (GABA) in microdialysis samples. However, OPA/sulfite derivatives have been reported to produce lower fluorescent yield than OPA derivatives using organic thiols as the nucleophile. To overcome this limitation we examined excitation and emission spectra, reaction time, pH, and concentration of reagents in the derivatization solution. Optimal detection parameters were determined as λex=220nm and λem=385nm for maximal fluorescence...
April 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28433593/tissue-distribution-and-metabolism-of-triadimefon-and-triadimenol-enantiomers-in-chinese-lizards-eremias-argus
#2
Jitong Li, Yinghuan Wang, Wei Li, Peng Xu, Baoyuan Guo, Jianzhong Li, Huili Wang
Triadimefon (TF, S-(+)-TF, R-(-)-TF) and its metabolite triadimenol (TN, TN-A1, A2 and TN-B1, B2) are two systemic fungicides and both of them are chiral pharmaceuticals which are widely used in agricultural industry. Many researches focused on the toxicity effects of triadimefon on mammals, while the ecotoxicological data of tiradimefon on reptiles is limited. In order to understand the toxicity mechanism of triadimefon in reptiles, the current study administrated S-(+)-TF or R-(-)-TF traidimefon (50mg/kg(bw)) to Chinese lizards (Eremias argus) respectively, the absorption, distribution of triadimefon and the formation of triadimenol were analysed at different sampling times...
April 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28433439/cardiac-amyloidosis-shows-decreased-diastolic-function-as-assessed-by-echocardiographic-parameterized-diastolic-filling
#3
Katrin Salman, Peter A Cain, Benjamin T Fitzgerald, Martin G Sundqvist, Martin Ugander
Cardiac amyloidosis is a rare but serious condition with poor survival. One of the early findings by echocardiography is impaired diastolic function, even before the development of cardiac symptoms. Early diagnosis is important, permitting initiation of treatment aimed at improving survival. The parameterized diastolic filling (PDF) formalism entails describing the left ventricular filling pattern during early diastole using the mathematical equation for the motion of a damped harmonic oscillator. We hypothesized that echocardiographic PDF analysis could detect differences in diastolic function between patients with amyloidosis and controls...
April 19, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28432714/high-human-cytomegalovirus-dnaemia-early-post-transplantation-associates-with-irreversible-and-progressive-loss-of-renal-function
#4
Wouter T Lollinga, Lilli Rurenga-Gard, Willem van Doesum, Rik van Bergen, Arjan Diepstra, Judith M Vonk, Annelies Riezebos-Brilman, Hgm Niesters, Willem J van Son, Jacob van den Born, Jan-Stephan Sanders
BACKGROUND: Transplant recipients are prone to viral infections, which could affect renal transplantation outcome. Our aim was to assess the effects of early human cytomegalovirus (CMV) DNAemia on transplant renal function. METHODS: 264 (age 50.9±13.5; male 55%) Renal transplantation recipients undergoing preemptive anti-CMV therapy were retrospectively categorized based on early (<3 months post-Tx) CMV peak viral load (PVL); PVL≤536, PVL536-6310 or PVL>6310 International Units/ml (IU/ml)...
April 22, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28432095/mushroom-emergence-detected-by-combining-spore-trapping-with-molecular-techniques
#5
Carles Castaño, Jonàs Oliva, Juan Martínez de Aragón, Josu G Alday, Javier Parladé, Joan Pera, José Antonio Bonet
Obtaining reliable and representative mushroom production data requires time-consuming sampling schemes. In this paper, we assessed a simple methodology to detect mushroom emergence by trapping the fungal spores of the fruiting body community in plots where mushroom production was determined weekly. We compared the performance of filter paper traps with that of funnel traps and combined these spore trapping methods with species-specific quantitative real-time PCR and Illumina MiSeq to determine the spore abundance...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28432058/mk5-haplodefficiency-attenuates-hypertrophy-and-preserves-diastolic-function-during-remodeling-induced-by-chronic-pressure-overload-in-the-mouse-heart
#6
Sherin Nawaito, Dharmendra Dingar, Pramod Sahadevan, Bahira Hussein, Fatiha Sahmi, Yanfen Shi, Marc-Antoine Gillis, Matthias Gaestel, Jean-Claude Tardif, Bruce G Allen
MK5 is a protein serine and threonine kinase that is activated by p38 MAPK and the atypical MAPKs ERK3 and ERK4. The physiological function(s) of MK5 remain unknown. Herein we examine the effect of MK5 haplodeficiency on cardiac function and myocardial remodeling. At 12-weeks of age, MK5 haplodeficient mice (MK5(+/-)) were smaller than age-matched wild-type litter mates (MK5(+/+)), with similar diastolic function but reduced systolic function. Transverse aortic constriction (TAC) was employed to induce a chronic pressure overload in twelve-week-old male MK5(+/-) and MK5(+/+) mice...
April 21, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28432032/neuronal-excitation-and-permeabilization-by-200-ns-pulsed-electric-field-an-optical-membrane-potential-study-with-fluovolt-dye
#7
Andrei G Pakhomov, Iurii Semenov, M Casciola, Shu Xiao
Electric field pulses of nano- and picosecond duration are a novel modality for neurostimulation, activation of Ca(2+) signaling, and tissue ablation. However it is not known how such brief pulses activate voltage-gated ion channels. We studied excitation and electroporation of hippocampal neurons by 200-ns pulsed electric field (nsPEF), by means of time-lapse imaging of the optical membrane potential (OMP) with FluoVolt dye. Electroporation abruptly shifted OMP to a more depolarized level, which was reached within <1ms...
April 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28431558/epidemiology-and-genotype-distribution-of-human-papillomavirus-hpv-in-southwest-china-a-cross-sectional-five-years-study-in-non-vaccinated-women
#8
Yishu Tang, Lan Zheng, Shuangshuang Yang, Bing Li, Huiting Su, Li-Ping Zhang
BACKGROUND: Large-size data on type-specific HPV prevalence in Southwest China are required to estimate the cervical cancer burden in the country and to prepare for HPV-based cervical screening program and further HPV vaccination of China. This HPV study is a pooled analysis of data from five years in Chongqing of China, which is cross-sectional in design using data collecting. RESULTS: The positivity of HPV was 26.2% (10542/40311), single type was 25.7% (10360/40311), multiple type was 8...
April 21, 2017: Virology Journal
https://www.readbyqxmd.com/read/28431362/rna-aptamer-based-electrochemical-aptasensor-for-c-reactive-protein-detection-using-functionalized-silica-microspheres-as-immunoprobes
#9
Junchun Wang, Jinjin Guo, Junjun Zhang, Wenjuan Zhang, Yuzhong Zhang
C-reactive protein (CRP) is a widely accepted biomarker of cardiovascular disease and inflammation. In this study, a RNA aptamer-based electrochemical sandwich type aptasensor for CRP detection was described using the functionalized silica microspheres as immunoprobes. Silica microspheres (Si MSs), which have good monodispersity and uniform shape, were firstly synthesized. The silica microspheres functionlized with gold nanoparticles (Au NPs) provided large surface area for immobilizing signal molecules (Zinc ions, Zn(2+)) and antibodies (Ab)...
April 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28431025/1h-magnetic-resonance-spectroscopy-of-live-human-sperm
#10
S Reynolds, S J Calvert, M N Paley, A A Pacey
STUDY QUESTION: Can 1H Magnetic Resonance Spectroscopy (MRS) be used to obtain information about the molecules and metabolites in live human spermatozoa? SUMMARY ANSWER: Percoll-based density gradient centrifugation (DGC) followed by a further two washing steps, yielded enough sperm with minimal contamination (<0.01%) from seminal fluid to permit effective MRS which detected significant differences (p < 0.05) in the choline/glycerophosphocholine, lipid and lactate regions of the 1H MRS spectrum between sperm in the pellet and those from the 40/80% interface...
April 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28430897/genetic-variants-associated-with-the-root-system-architecture-of-oilseed-rape-brassica-napus-l-under-contrasting-phosphate-supply
#11
Xiaohua Wang, Yanling Chen, Catherine L Thomas, Guangda Ding, Ping Xu, Dexu Shi, Fabian Grandke, Kemo Jin, Hongmei Cai, Fangsen Xu, Bin Yi, Martin R Broadley, Lei Shi
Breeding crops with ideal root system architecture for efficient absorption of phosphorus is an important strategy to reduce the use of phosphate fertilizers. To investigate genetic variants leading to changes in root system architecture, 405 oilseed rape cultivars were genotyped with a 60K Brassica Infinium SNP array in low and high P environments. A total of 285 single-nucleotide polymorphisms were associated with root system architecture traits at varying phosphorus levels. Nine single-nucleotide polymorphisms corroborate a previous linkage analysis of root system architecture quantitative trait loci in the BnaTNDH population...
April 19, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28430507/beating-the-standard-sensitivity-bandwidth-limit-of-cavity-enhanced-interferometers-with-internal-squeezed-light-generation
#12
M Korobko, L Kleybolte, S Ast, H Miao, Y Chen, R Schnabel
The shot-noise limited peak sensitivity of cavity-enhanced interferometric measurement devices, such as gravitational-wave detectors, can be improved by increasing the cavity finesse, even when comparing fixed intracavity light powers. For a fixed light power inside the detector, this comes at the price of a proportional reduction in the detection bandwidth. High sensitivity over a large span of signal frequencies, however, is essential for astronomical observations. It is possible to overcome this standard sensitivity-bandwidth limit using nonclassical correlations in the light field...
April 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28430367/effect-of-dalteparin-administration-on-thrombin-generation-kinetics-in-healthy-dogs
#13
Carolyn Gara-Boivin, Jérôme R E Del Castillo, Marilyn E Dunn, Christian Bédard
BACKGROUND: Dalteparin is used to prevent thrombotic complications in dogs. Measurement of anti-factor Xa (anti-FXa) activity is currently used for monitoring therapy, but remains a nonfunctional test. The calibrated automated thrombogram (CAT) could be a suitable approach for functional monitoring. OBJECTIVES: We hypothesized that the CAT will detect decreased endogenous thrombin potential (ETP) in healthy dogs receiving dalteparin. METHODS: Twenty-four healthy adult Beagles were randomly allocated to 4 equal groups...
April 21, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28430235/fiber-bragg-grating-interrogation-using-wavelength-modulated-tunable-distributed-feedback-lasers-and-a-fiber-optic-mach-zehnder-interferometer
#14
Anirban Roy, Arup Lal Chakraborty, Chandan Kumar Jha
This paper demonstrates a technique of high-resolution interrogation of two fiber Bragg gratings (FBGs) with flat-topped reflection spectra centered on 1649.55 nm and 1530.182 nm with narrow line width tunable semiconductor lasers emitting at 1651.93 nm and 1531.52 nm, respectively. The spectral shift of the reflection spectrum in response to temperature and strain is accurately measured with a fiber-optic Mach-Zehnder interferometer that has a free spectral range of 0.0523 GHz and a broadband photodetector...
April 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/28430229/light-scattering-from-normal-and-cervical-cancer-cells
#15
Xiaogang Lin, Nan Wan, Lingdong Weng, Yong Zhou
The light scattering characteristic plays a very important role in optic imaging and diagnostic applications. For optical detection of the cell, cell scattering characteristics have an extremely vital role. In this paper, we use the finite-difference time-domain (FDTD) algorithm to simulate the propagation and scattering of light in biological cells. The two-dimensional scattering cell models were set up based on the FDTD algorithm. The cell models of normal cells and cancerous cells were established, and the shapes of organelles, such as mitochondria, were elliptical...
April 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/28430109/acquisition-of-electrocardiogram-signals-during-magnetic-resonance-imaging
#16
Julien Oster, Gari Clifford
The recording of the electrocardiogram (ECG) during Magnetic Resonance Imaging (MRI) acquisition is of great interest and importance. Firstly, MRI acquisition is a relatively slow process, which therefore complicates the imaging of moving organs. Cardiac MRI requires the development of strategies for acquiring high quality images, which is mainly achieved by synchronising the image acquisition with a specific time during the cardiac cycle. The ECG is used to monitor the heart's activity, and the detection of the largest and steepest peak in the cardiac cycle (the QRS complex) triggers the acquisition of slices of the k-space...
April 21, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28429353/roles-of-the-nrf2-ho-1-pathway-in-the-anti-oxidative-stress-response-to-ischemia-reperfusion-brain-injury-in-rats
#17
L-J Jiang, S-M Zhang, C-W Li, J-Y Tang, F-Y Che, Y-C Lu
OBJECTIVE: The aim of this study was to investigate the roles of the Nrf2/HO-1 pathway in the responses to the oxidative stress created by ischemia-reperfusion brain injury in rats. MATERIALS AND METHODS: 54 healthy, adult, male SD rats were included in the study. Eighteen (18) rats were placed in the sham group. The ischemia-reperfusion model was created in the other 36 rats, among which 18 received injections of Nrf2 agonist before the surgery. The suture method was used to create artery occlusions in the right brain of the rats; and reperfusion was done after 90-minute ischemia (MCAO); while no suture was inserted in the sham group...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28428563/a-sublethal-dose-of-a-neonicotinoid-insecticide-disrupts-visual-processing-and-collision-avoidance-behaviour-in-locusta-migratoria
#18
Rachel H Parkinson, Jacelyn M Little, John R Gray
Neonicotinoids are known to affect insect navigation and vision, however the mechanisms of these effects are not fully understood. A visual motion sensitive neuron in the locust, the Descending Contralateral Movement Detector (DCMD), integrates visual information and is involved in eliciting escape behaviours. The DCMD receives coded input from the compound eyes and monosynaptically excites motorneurons involved in flight and jumping. We show that imidacloprid (IMD) impairs neural responses to visual stimuli at sublethal concentrations, and these effects are sustained two and twenty-four hours after treatment...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428549/low-noise-time-resolved-optical-sensing-of-electromagnetic-pulses-from-petawatt-laser-matter-interactions
#19
T S Robinson, F Consoli, S Giltrap, S J Eardley, G S Hicks, E J Ditter, O Ettlinger, N H Stuart, M Notley, R De Angelis, Z Najmudin, R A Smith
We report on the development and deployment of an optical diagnostic for single-shot measurement of the electric-field components of electromagnetic pulses from high-intensity laser-matter interactions in a high-noise environment. The electro-optic Pockels effect in KDP crystals was used to measure transient electric fields using a geometry easily modifiable for magnetic field detection via Faraday rotation. Using dielectric sensors and an optical fibre-based readout ensures minimal field perturbations compared to conductive probes and greatly limits unwanted electrical pickup between probe and recording system...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428084/metabolomics-of-fescue-toxicosis-in-grazing-beef-steers
#20
Ryan S Mote, Nicholas S Hill, Karan Uppal, ViLinh T Tran, Dean P Jones, Nikolay M Filipov
Fescue toxicosis (FT) results from consumption of tall fescue (Lolium arundinaceum) infected with an endophyte (Epichloë coenophiala) that produces ergot alkaloids (EA), which are considered key etiological agents of FT. Decreased weight gains, hormonal imbalance, circulating cholesterol disruption, and decreased volatile fatty acid absorption suggest toxic (E+) fescue-induced metabolic perturbations. Employing untargeted high-resolution metabolomics (HRM) to analyze E+ grazing-induced plasma and urine metabolome changes, fescue-naïve Angus steers were placed on E+ or non-toxic (Max-Q) fescue pastures and plasma and urine were sampled before, 1, 2, 14, and 28 days after pasture assignment...
April 18, 2017: Food and Chemical Toxicology
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