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https://www.readbyqxmd.com/read/28438305/cardiovascular-response-to-recreational-hockey-in-middle-aged-men
#1
Zack A Goodman, Scott G Thomas, Robert C Wald, Jack M Goodman
The present study examined the hemodynamic response to recreational pick-up hockey relative to maximal exercise testing in middle-aged men. A total of 23 men with a mean age of 53 ± 7 years were studied. Graded exercise testing on a cycle ergometer determined maximal oxygen consumption, blood pressure (BP), and heart rate (HR). Ambulatory BP and Holter electrocardiographic monitoring was performed during one of their weekly hockey games (mean duration = 45 ± 7.2 minutes): for "On-Ice" responses (PLAY; data recorded while standing immediately after a shift; 8...
April 11, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/28371585/validity-of-field-assessments-to-predict-peak-muscle-power-in-preschoolers
#2
Sara King-Dowling, Nicole A Proudfoot, John Cairney, Brian W Timmons
BACKGROUND: Field-based fitness assessments are time- and cost-efficient. However, no studies to date have reported the predictive value of field-based musculoskeletal fitness assessments in preschoolers. The purpose of this study was to determine the validity of two field assessments to predict peak muscle power in preschool-aged children. METHODS: Four-hundred nineteen 3- to 5-year olds participated (208 girls, 211 boys; mean age: 4.5±0.9 years). Peak power (PP) was evaluated using a modified 10-second Wingate protocol as the criterion standard...
April 3, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28355412/influence-of-work-pace-on-upper-extremity-kinematics-and-muscle-activity-in-a-short-cycle-repetitive-pick-and-place-task
#3
Tessy Luger, Svend Erik Mathiassen, Divya Srinivasan, Tim Bosch
Objectives: This study investigated the extent to which controlled changes in work pace in a cyclic pick-and-place task influence upper extremity kinematics and muscle activity, and whether an effect depends on working height. Methods: Thirteen participants performed the task for 4 min at each of five work paces ranging from 8 to 12 cycles·min-1 in each of two experimental conditions where the hand was moved horizontally with an average upper arm elevation of 30° and 50°, respectively...
February 10, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28353646/intraguild-predation-dynamics-in-a-lake-ecosystem-based-on-a-coupled-hydrodynamic-ecological-model-the-example-of-lake-kinneret-israel
#4
Vardit Makler-Pick, Matthew R Hipsey, Tamar Zohary, Yohay Carmel, Gideon Gal
The food web of Lake Kinneret contains intraguild predation (IGP). Predatory invertebrates and planktivorous fish both feed on herbivorous zooplankton, while the planktivorous fish also feed on the predatory invertebrates. In this study, a complex mechanistic hydrodynamic-ecological model, coupled to a bioenergetics-based fish population model (DYCD-FISH), was employed with the aim of revealing IGP dynamics. The results indicate that the predation pressure of predatory zooplankton on herbivorous zooplankton varies widely, depending on the season...
March 29, 2017: Biology
https://www.readbyqxmd.com/read/28327092/metax-a-flexible-and-comprehensive-software-for-processing-metabolomics-data
#5
Bo Wen, Zhanlong Mei, Chunwei Zeng, Siqi Liu
BACKGROUND: Non-targeted metabolomics based on mass spectrometry enables high-throughput profiling of the metabolites in a biological sample. The large amount of data generated from mass spectrometry requires intensive computational processing for annotation of mass spectra and identification of metabolites. Computational analysis tools that are fully integrated with multiple functions and are easily operated by users who lack extensive knowledge in programing are needed in this research field...
March 21, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28295580/specpad-device-independent-nmr-data-visualization-and-processing-based-on-the-novel-dart-programming-language-and-html5-web-technology
#6
Bruno Guigas
SpecPad is a new device-independent software program for the visualization and processing of one- and two-dimensional NMR time domain (FID) and frequency domain (spectrum) data. It is the result of a project to investigate whether the novel programming language DART, in combination with Html5 Web technology, forms a suitable base to write an NMR data evaluation software which runs on modern computing devices such as Android, iOS, and Windows tablets as well as on Windows, Linux, and Mac OS X desktop PCs and notebooks...
March 12, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28286574/nontargeted-homologue-series-extraction-from-hyphenated-high-resolution-mass-spectrometry-data
#7
Martin Loos, Heinz Singer
BACKGROUND: A large proportion of polar anthropogenic compounds routinely released into the environment comprises homologue series, i.e., sets of chemicals differing in a repeating chemical unit. Using analytical techniques such as liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS), these compounds are readily measurable as signal sets with characteristic differences in mass and typically retention time. However, and despite such distinct characteristics, no computational approach for the direct, simultaneous and untargeted detection of all such signal sets comprising both LC and HRMS information has to date been presented...
2017: Journal of Cheminformatics
https://www.readbyqxmd.com/read/28213793/automation-of-peak-tracking-analysis-of-stepwise-perturbed-nmr-spectra
#8
Tommaso Banelli, Marco Vuano, Federico Fogolari, Andrea Fusiello, Gennaro Esposito, Alessandra Corazza
We describe a new algorithmic approach able to automatically pick and track the NMR resonances of a large number of 2D NMR spectra acquired during a stepwise variation of a physical parameter. The method has been named Trace in Track (TINT), referring to the idea that a gaussian decomposition traces peaks within the tracks recognised through 3D mathematical morphology. It is capable of determining the evolution of the chemical shifts, intensity and linewidths of each tracked peak.The performances obtained in term of track reconstruction and correct assignment on realistic synthetic spectra were high above 90% when a noise level similar to that of experimental data were considered...
February 17, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28213211/application-of-2d-hplc-coupled-with-principal-component-analysis-to-study-an-industrial-opiate-processing-stream
#9
Paul G Stevenson, Niki K Burns, Stuart D Purcell, Paul S Francis, Neil W Barnett, Fiona Fry, Xavier A Conlan
Principal component analysis (PCA) loading plots were used to elucidate key differences between two-dimensional high performance liquid chromatography (2D-HPLC) fingerprint data from samples collected from stages along the Papaver somniferum industrial process chemistry workflow. Data reduction was completed using a 2D-HPLC peak picking approach as a precursor to chemometric analysis. Using comparisons of the final stages of product extraction as an example, PCA analysis of characteristic 2D-HPLC fingerprints accounted for 84...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28160196/infos-spectrum-fitting-software-for-nmr-analysis
#10
Albert A Smith
Software for fitting of NMR spectra in MATLAB is presented. Spectra are fitted in the frequency domain, using Fourier transformed lineshapes, which are derived using the experimental acquisition and processing parameters. This yields more accurate fits compared to common fitting methods that use Lorentzian or Gaussian functions. Furthermore, a very time-efficient algorithm for calculating and fitting spectra has been developed. The software also performs initial peak picking, followed by subsequent fitting and refinement of the peak list, by iteratively adding and removing peaks to improve the overall fit...
February 3, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28160195/peak-picking-multidimensional-nmr-spectra-with-the-contour-geometry-based-algorithm-cypick
#11
Julia M Würz, Peter Güntert
The automated identification of signals in multidimensional NMR spectra is a challenging task, complicated by signal overlap, noise, and spectral artifacts, for which no universally accepted method is available. Here, we present a new peak picking algorithm, CYPICK, that follows, as far as possible, the manual approach taken by a spectroscopist who analyzes peak patterns in contour plots of the spectrum, but is fully automated. Human visual inspection is replaced by the evaluation of geometric criteria applied to contour lines, such as local extremality, approximate circularity (after appropriate scaling of the spectrum axes), and convexity...
February 3, 2017: Journal of Biomolecular NMR
https://www.readbyqxmd.com/read/28150425/integrated-hptlc-based-methodology-for-the-tracing-of-bioactive-compounds-in-herbal-extracts-employing-multivariate-chemometrics-a-case-study-on-morus-alba
#12
Eliza Chaita, Evagelos Gikas, Nektarios Aligiannis
INTRODUCTION: In drug discovery, bioassay-guided isolation is a well-established procedure, and still the basic approach for the discovery of natural products with desired biological properties. However, in these procedures, the most laborious and time-consuming step is the isolation of the bioactive constituents. A prior identification of the compounds that contribute to the demonstrated activity of the fractions would enable the selection of proper chromatographic techniques and lead to targeted isolation...
March 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28012801/occurrence-and-variation-of-lipophilic-shellfish-toxins-in-phytoplankton-shellfish-and-seawater-samples-from-the-aquaculture-zone-in-the-yellow-sea-china
#13
Meihui Li, Geng Sun, Jiangbing Qiu, Aifeng Li
Lipophilic shellfish toxins (LSTs) produced by diverse microalgae have become a potential threat to the marine aquaculture industry in coastal regions throughout the world. In this study, phytoplankton, scallop, and seawater samples were collected and monitored in the aquaculture zone of Liuqing Bay located in the coast of Qingdao, Yellow Sea, in 2014 and 2015. Dissolved LSTs in seawater were adsorbed by solid phase adsorption toxin tracking (SPATT) bags and solid phase extraction (SPE) cartridges, respectively...
December 22, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27966901/multiplexed-post-experimental-monoisotopic-mass-refinement-mpe-mmr-to-increase-sensitivity-and-accuracy-in-peptide-identifications-from-tandem-mass-spectra-of-cofragmentation
#14
Inamul Hasan Madar, Seung-Ik Ko, Hokeun Kim, Dong-Gi Mun, Sangtae Kim, Richard D Smith, Sang-Won Lee
Mass spectrometry (MS)-based proteomics, which uses high-resolution hybrid mass spectrometers such as the quadrupole-orbitrap mass spectrometer, can yield tens of thousands of tandem mass (MS/MS) spectra of high resolution during a routine bottom-up experiment. Despite being a fundamental and key step in MS-based proteomics, the accurate determination and assignment of precursor monoisotopic masses to the MS/MS spectra remains difficult. The difficulties stem from imperfect isotopic envelopes of precursor ions, inaccurate charge states for precursor ions, and cofragmentation...
December 22, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27924495/autopiquer-a-robust-and-reliable-peak-detection-algorithm-for-mass-spectrometry
#15
David P A Kilgour, Sam Hughes, Samantha L Kilgour, C Logan Mackay, Magnus Palmblad, Bao Quoc Tran, Young Ah Goo, Robert K Ernst, David J Clarke, David R Goodlett
We present a simple algorithm for robust and unsupervised peak detection by determining a noise threshold in isotopically resolved mass spectrometry data. Solving this problem will greatly reduce the subjective and time-consuming manual picking of mass spectral peaks and so will prove beneficial in many research applications. The Autopiquer approach uses autocorrelation to test for the presence of (isotopic) structure in overlapping windows across the spectrum. Within each window, a noise threshold is optimized to remove the most unstructured data, whilst keeping as much of the (isotopic) structure as possible...
December 6, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27908224/serum-substance-p-concentrations-to-predict-oocyte-maturation-index-and-clinical-pregnancy
#16
Yavuz Sahin, Enis Özkaya, Semra Kayatas Eser, Tayfun Kutlu, Ilhan Sanverdi, Gulden Tunali, Ates Karateke
AIM: The aim of this study was to assess the predictive value of serum substance P (SP) concentrations on oocyte maturation and clinical pregnancy. METHODS: Ninety-three women with unexplained infertility underwent intracytoplasmic sperm injection (ICSI) cycles. Antagonist protocol was started for each participant and at the day of oocyte pick up, serum samples were obtained from each participant to assess SP concentrations, and these concentrations were utilized to predict mature/total oocyte ratio and clinical pregnancy...
March 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27900236/identification-of-two-sulfated-cholesterol-metabolites-found-in-the-urine-of-a-patient-with-niemann-pick-disease-type-c-as-novel-candidate-diagnostic-markers
#17
Masamitsu Maekawa, Kaoru Omura, Shoutaro Sekiguchi, Takashi Iida, Daisuke Saigusa, Hiroaki Yamaguchi, Nariyasu Mano
In the urine of a Niemann-Pick disease type C (NPC) patient, we have identified three characteristic intense peaks that have not been observed in the urine of a 3β-hydroxysteroid-Δ(5)-C27-steroid dehydrogenase deficiency patient or a healthy infant and adult. Based on accurate masses of the protonated molecules, we focused on two of them as candidate NPC diagnostic markers. Two synthesized authentic preparations agreed with the two compounds found in NPC patient urine in regard to both chromatographic behavior and accurate masses of the deprotonated molecules...
2016: Mass Spectrometry
https://www.readbyqxmd.com/read/27865939/c-reactive-protein-response-is-higher-in-early-than-in-late-ovarian-hyperstimulation-syndrome
#18
COMPARATIVE STUDY
Kati V M Korhonen, Hanna M Savolainen-Peltonen, Tomi S Mikkola, Aila E Tiitinen, Leila S Unkila-Kallio
OBJECTIVES: Many in vitro fertilization (IVF) complications are inflammatory by nature, some of which are even life-threatening. We evaluated the response of C-reactive protein (CRP) in IVF complications, especially in early and late ovarian hyperstimulation syndrome (OHSS), to support clinical decision making in gynecological emergency policlinics. STUDY DESIGN: In a prospective two-year study at Helsinki University Hospital, Finland, we recruited patients with IVF complications including moderate or severe OHSS (n=47 patients: 36 early and 14 late OHSS cases), or other IVF complications (n=13)...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27853180/piezoelectric-extraction-of-ecg-signal
#19
Mahmoud Al Ahmad
The monitoring and early detection of abnormalities or variations in the cardiac cycle functionality are very critical practices and have significant impact on the prevention of heart diseases and their associated complications. Currently, in the field of biomedical engineering, there is a growing need for devices capable of measuring and monitoring a wide range of cardiac cycle parameters continuously, effectively and on a real-time basis using easily accessible and reusable probes. In this paper, the revolutionary generation and extraction of the corresponding ECG signal using a piezoelectric transducer as alternative for the ECG will be discussed...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27746230/quantitative-evaluation-of-orofacial-motor-function-in-mice-the-pasta-gnawing-test-a-voluntary-and-stress-free-behavior-test
#20
R Rabl, A Horvath, C Breitschaedel, S Flunkert, H Roemer, B Hutter-Paier
BACKGROUND: Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals. NEW METHOD: To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piece of spaghetti. Two parameters were evaluated, the biting speed and the biting peaks per biting episode. To evaluate the power of this test compared to commonly used limb motor and muscle strength tests, three mouse models of Parkinson's disease, amyotrophic lateral sclerosis and Niemann-Pick disease were tested in the pasta gnawing test, RotaRod and wire suspension test...
December 1, 2016: Journal of Neuroscience Methods
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