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https://www.readbyqxmd.com/read/28936216/genetic-tracing-of-jatropha-curcas-l-from-its-mesoamerican-origin-to-the-world
#1
Haiyan Li, Suguru Tsuchimoto, Kyuya Harada, Masanori Yamasaki, Hiroe Sakai, Naoki Wada, Atefeh Alipour, Tomohiro Sasai, Atsushi Tsunekawa, Hisashi Tsujimoto, Takayuki Ando, Hisashi Tomemori, Shusei Sato, Hideki Hirakawa, Victor P Quintero, Alfredo Zamarripa, Primitivo Santos, Adel Hegazy, Abdalla M Ali, Kiichi Fukui
Jatropha curcas L. (Jatropha), a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using 246 accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28932349/novel-synthetic-adhesive-as-an-effective-alternative-to-fibrin-based-adhesives
#2
Pramod Kadaba Srinivasan, Vera Sperber, Mamdouh Afify, Hirokazu Tanaka, Kenji Fukushima, Babette Kögel, Felix Gremse, René Tolba
AIM: To compare a novel, fully synthetic, polyurethane based glue (MAR-1) to fibrin sealant in a partial liver resection rat model. METHODS: After 50% resection of the lateral left liver lobe in male Wistar rats (n = 7/group/time point), MAR-1, Fibrin or NaCl was applied. After 14, 21 and 90 postoperative days, sealant degradation, intra-abdominal adhesions were scored, and histological examination of liver tissue was performed. RESULTS: (Mean ± SEM) (MAR-1 vs Fibrin vs NaCl)...
August 28, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28927938/hollow-porous-molecularly-imprinted-polymer-for-highly-selective-clean-up-followed-by-influential-preconcentration-of-ultra-trace-glibenclamide-from-bio-fluid
#3
Abbas Ostovan, Mehrorang Ghaedi, Maryam Arabi, Arash Asfaram
In the present work, hollow porous molecularly imprinted polymer (HPMIP) was prepared via adopting a sacrificial support approach using glibenclamide (GB) as template, methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate as cross-linker (EGDMA) and mesoporous MCM-48 nanospheres as support. Owing to a short thickness of the foresaid HPMIP, a suitable steric structure was readily available that lead to fast and effective mass transfer of target analyte to sorbent and consequently supply high binding capacity...
September 10, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28926682/development-of-a-simple-and-valid-method-for-the-trace-determination-of-phthalate-esters-in-human-plasma-using-dispersive-liquid-liquid-microextraction-coupled-with-gc-ms
#4
Karim Ebrahim, Parinaz Poursafa, Mohammad Mehdi Amin
A new method was developed for the trace determination of phthalic acid esters in plasma using dispersive liquid-liquid microextraction and gas chromatography with mass spectrometry analysis. Plasma proteins were efficiently precipitated by trichloroacetic acid and then a mixture of chlorobenzene (as extraction solvent) and acetonitrile (as dispersive solvent) rapidly injected to clear supernatant using a syringe. After the centrifuging, chlorobenzene were sedimented in the bottom of the test tube. 1 μL of this sedimented phase was injected into the gas chromatograph for phthalic acid esters analysis...
September 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28923752/anthropogenic-and-geogenic-mass-input-of-trace-elements-to-moss-and-natural-surface-soil-in-norway
#5
Erik R Christensen, Eiliv Steinnes, Ola Anfin Eggen
Data sets for concentrations of up to 22 elements including Pb, Cd, Ag, As, and Hg and several rare earth elements (REEs) in moss and surface soil from all over mainland Norway are analyzed by positive matrix factorization (PMF) and principal component analysis (PCA) with centered log-ratio transformation. Moss and soil samples collected in 2010 and 2005, respectively, show both a distinct long-range atmospheric transport PMF factor including a dominant Pb loading along with smaller loadings of Mo, Cd, Sb, and As, and a geogenic factor dominated by Ce, La, and Y...
September 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28920824/baseline-health-and-nutrition-evaluation-of-two-sympatric-nocturnal-lemur-species-avahi-laniger-and-lepilemur-mustelinus-residing-near-an-active-mine-site-at-ambatovy-madagascar
#6
Randall E Junge, Cathy V Williams, Hajanirina Rakotondrainibe, Karine L Mahefarisoa, Tsiky Rajaonarivelo, Charles Faulkner, Vanessa Mass
Extractive industries can have significant impacts on ecosystems through loss of habitat, degradation of water quality, and direct impact on floral and faunal biodiversity. When operations are located in sensitive regions with high biodiversity containing endangered or threatened species, it is possible to minimize impact on the environment by developing programs to scientifically monitor the impact on resident flora and fauna species in the early phases of operation so that effects can be mitigated whenever possible...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28920599/magnetic-nanoparticle-enhanced-surface-plasmon-resonance-biosensor-for-extracellular-vesicle-analysis
#7
Agnes T Reiner, Nicolas-Guillermo Ferrer, Priyamvada Venugopalan, Ruenn Chai Lai, Sai Kiang Lim, Jakub Dostálek
The sensitive analysis of small lipid extracellular vesicles (EVs) by using a grating-coupled surface plasmon resonance (GC-SPR) biosensor has been reported. In order to enable the analysis of trace amounts of EVs present in complex liquid samples, the target analyte is pre-concentrated on the sensor surface by using magnetic nanoparticles and its affinity binding is probed by wavelength interrogation of SPR. The GC-SPR has been demonstrated to allow for the implementation of efficient pulling of EVs to the sensor surface by using magnetic nanoparticles and an external magnetic field gradient applied through the sensor chip...
September 18, 2017: Analyst
https://www.readbyqxmd.com/read/28918329/an-integrated-strategy-for-rapid-discovery-and-identification-of-the-sequential-piperine-metabolites-in-rats-using-ultra-high-performance-liquid-chromatography-high-resolution-mass-spectrometery
#8
Zhanpeng Shang, Wei Cai, Yanfeng Cao, Fei Wang, Zhibin Wang, Jianqiu Lu, Jiayu Zhang
Piperine, one of the major bioactive constituents isolated from natural flavorings and medicinal-culinary herbs, possesses various biological activities. In the present study, an integrated strategy based on ultra high-performance liquid chromatography/high resolution mass spectrometry was established to reveal piperine metabolism in rats. First of all, post-acquisition data-mining methods, including high resolution extracted ion chromatograms (HREICs) and multiple mass defect filtering (MMDF), were used to screen piperine metabolite candidates in a full-scan HRMS(1) level...
September 9, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28918328/semiautomated-determination-of-neonicotinoids-and-characteristic-metabolite-in-urine-samples-using-turboflow%C3%A2-coupled-to-ultra-high-performance-liquid-chromatography-coupled-to-orbitrap-analyzer
#9
Marina López-García, Roberto Romero-González, Marina Lacasaña, Antonia Garrido Frenich
A semiautomated method based on ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high resolution mass spectrometry has been developed for the determination of neonicotinoids (imidacloprid, acetamiprid, clothianidin, dinotefuran, nitenpyram, thiacloprid and thiamethoxam) and the metabolite acetamiprid-n-desmethyl in urine samples. Two automated methods were tested (solid-phase extraction "SPE" and turbulent flow chromatography "TurboFlow™"), obtaining the best results when TurboFlow™ was applied...
September 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28918137/reticulo-rumen-mass-epithelium-gene-expression-and-systemic-biomarkers-of-metabolism-and-inflammation-in-holstein-dairy-cows-fed-a-high-energy-diet
#10
J M Arroyo, A Hosseini, Z Zhou, A Alharthi, E Trevisi, J S Osorio, J J Loor
Feeding a higher-energy diet by increasing cereal grains at the expense of forage during the last 3 to 4 wk prepartum is a traditional approach to help the rumen "adapt" to the traditional diets fed at the onset of lactation. Increasing grain/concentrate in the diet changes ruminal fermentation and in sheep and goats elicits marked changes in mRNA expression of immune-related genes in ruminal epithelium. Whether such changes at the epithelial and systemic levels occur in dairy cows when the dietary energy content increases at a fixed level of concentrate is unknown...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917749/trace-determination-of-volatile-polycyclic-aromatic-hydrocarbons-in-natural-waters-by-magnetic-ionic-liquid-based-stir-bar-dispersive-liquid-microextraction
#11
Juan L Benedé, Jared L Anderson, Alberto Chisvert
In this work, a novel hybrid approach called stir bar dispersive liquid microextraction (SBDLME) that combines the advantages of stir bar sorptive extraction (SBSE) and dispersive liquid-liquid microextraction (DLLME) has been employed for the accurate and sensitive determination of ten polycyclic aromatic hydrocarbons (PAHs) in natural water samples. The extraction is carried out using a neodymium stir bar magnetically coated with a magnetic ionic liquid (MIL) as extraction device, in such a way that the MIL is dispersed into the solution at high stirring rates...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917730/direct-and-comprehensive-analysis-of-dyes-based-on-integrated-molecular-and-structural-information-via-laser-desorption-laser-postionization-mass-spectrometry
#12
Rong Liu, Zhibin Yin, Yixin Leng, Wei Hang, Benli Huang
Laser desorption laser postionization time-of-flight mass spectrometry (LDPI-TOFMS) was employed for direct analysis and determination of typical basic dyes. It was also used for the analysis and comprehensive understanding of complex materials such as blue ballpoint pen inks. Simultaneous emergences of fragmental and molecular information largely simplify and facilitate unambiguous identification of dyes via variable energy of 266nm postionization laser. More specifically, by optimizing postionization laser energy with the same energy of desorption laser, the structurally significant results show definite differences in the fragmentation patterns, which offer opportunities for discrimination of isomeric species with identical molecular weight...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917047/detection-of-selenoproteins-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry-la-icp-ms-in-immobilized-ph-gradient-ipg-strips
#13
Jordan Sonet, Sandra Mounicou, Laurent Chavatte
In contrast to other trace elements that are cofactors of enzymes and removed from proteins under denaturing conditions, Se is covalently bound to proteins when incorporated into selenoproteins, since it is a component of selenocysteine aminoacid. It implies that selenoproteins can undergo several biochemical separation methods in stringent and chaotropic conditions and still maintain the presence of selenium in the primary sequence. This feature has been used to develop a method for the detection of trace levels of human selenoproteins in cell extracts without the use of radioactive isotopes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28917046/nonradioactive-isotopic-labeling-and-tracing-of-selenoproteins-in-cultured-cell-lines
#14
Jordan Sonet, Sandra Mounicou, Laurent Chavatte
Selenium (Se) is an essential component of genetically encoded selenoproteins, in the form of a rare amino acid, namely the selenocysteine (Sec). Radioactive (75)Se has been widely used to trace selenoproteins in vitro and in vivo (cell models and animals). Alternatively, its unique isotopic pattern can be used to detect and characterize nonradioactive Se-compounds in cellular extracts using molecular or elemental mass spectrometry at ppm levels. However, when studying trace levels of Se-compounds, such as selenoproteins (ppt levels), the distribution of the signal between its six naturally abundant isotopes reduces its sensitivity...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28916349/offshore-iron-sand-extraction-in-new-zealand-potential-trace-metal-exposure-of-benthic-and-pelagic-biota
#15
Kay Vopel, Chris Pook, Peter Wilson, John Robertson
Plans to exploit an offshore source of iron sand in South Taranaki Bight (STB), New Zealand, caused concerns that such exploitation may expose benthic and pelagic biota to elevated trace metal concentrations. We conducted dilute-acid extractions and standard elutriate tests to investigate the potential of this exploitation to (1) create a new seafloor with elevated trace metal content, (2) mobilise trace metals during iron sand extraction and, (3) enrich the returning process seawater, which feeds iron sand through mills, with trace metals...
September 12, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28916097/determination-of-99-tc-in-fresh-water-using-tru-resin-by-icp-ms
#16
Nicolas Guérin, Remi Riopel, Sheila Kramer-Tremblay, Nimal de Silva, Jack Cornett, Xiongxin Dai
Technetium-99 ((99)Tc) determination at trace level by inductively coupled plasma mass spectrometry (ICP-MS) is challenging because there is no readily available appropriate Tc isotopic tracer. A new method using Re as a recovery tracer to determine (99)Tc in fresh water samples, which does not require any evaporation step, was developed. Tc(VII) and Re(VII) were pre-concentrated on a small anion exchange resin (AER) cartridge from one litre of water sample. They were then efficiently eluted from the AER using a potassium permanganate (KMnO4) solution...
October 2, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28915467/the-broad-emission-at-785nm-in-yag-ce-3-cr-3-phosphor
#17
Xianju Zhou, Xiaoxia Luo, Bing Wu, Sha Jiang, Li Li, Xiaobing Luo, Yu Pang
Luminescence of defects and/or impurities is important for phosphors. One broad emission band centered at 785nm was observed in (Y0.97Ce0.03)3(Al1-xCrx)5O12 (x=0.005, 0.01, 0.03, 0.06, 0.09), (YAG:Ce,Cr) nano-particles synthesized by polymer-assisted sol-gel method in this work. In order to study the source of this wide emission band, the crystalline phase structure of the phosphors was examined by X-ray diffraction (XRD). No obvious diffraction peaks due to impurity phase were observed even though the calcining temperature of the samples arrived at 1400°C...
September 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28914752/ct-attenuation-of-pericoronary-adipose-tissue-in-normal-versus-atherosclerotic-coronary-segments-as-defined-by-intravascular-ultrasound
#18
Mohamed Marwan, Michaela Hell, Annika Schuhbäck, Sören Gauss, Daniel Bittner, Tobias Pflederer, Stephan Achenbach
BACKGROUND: The factors influencing genesis of atherosclerosis at specific regions within the coronary arterial system are currently uncertain. Local mechanical factors such as shear stress as well as metabolic factors, including inflammatory mediators released from epicardial fat, have been proposed. We analyzed computed tomographic (CT) attenuation of pericoronary adipose tissue in normal versus atherosclerotic coronary segments as defined by intravascular ultrasound (IVUS). PATIENTS AND METHODS: We evaluated the data sets of 29 patients who were referred for invasive coronary angiography and in whom IVUS of 1 coronary vessel was performed for clinical reasons...
September 2017: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/28913542/metal-release-profiles-of-orthodontic-bands-brackets-and-wires-an-in-vitro-study
#19
B Wendl, H Wiltsche, E Lankmayr, H Winsauer, A Walter, A Muchitsch, N Jakse, M Wendl, T Wendl
AIM: The present study evaluated the temporal release of Co Cr, Mn, and Ni from the components of a typical orthodontic appliance during simulated orthodontic treatment. MATERIALS AND METHODS: Several commercially available types of bands, brackets, and wires were exposed to an artificial saliva solution for at least 44 days and the metals released were quantified in regular intervals using inductively coupled plasma quadrupole mass spectrometry (ICP-MS, Elan DRC+, Perkin Elmer, USA)...
September 14, 2017: Journal of Orofacial Orthopedics, Fortschritte der Kieferorthopädie
https://www.readbyqxmd.com/read/28911658/identification-of-an-exposure-risk-to-heavy-metals-from-pharmaceutical-grade-rubber-stoppers
#20
Xianghui Li, Pingping Qian
Exposure to low concentrations of heavy metals and metalloids represents a well-documented risk to animal and human health. However, current standards (European Pharmacopeia [EP], United States Pharmacopoeia [USP], International Organization for Standardization [ISO], YBB concerned with rubber closures) only require testing for Zn in pharmaceutical-grade rubber stoppers and then using only pure water as a solvent. We extracted and quantified heavy metals and trace elements from pharmaceutical-grade rubber stoppers under conditions that might occur during the preparation of drugs...
July 2017: Journal of Food and Drug Analysis
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