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https://www.readbyqxmd.com/read/28641226/evaluation-of-trace-element-concentration-in-cancerous-and-non-cancerous-tissues-of-human-stomach
#1
Shadi Kohzadi, Farshad Sheikhesmaili, Ramesh Rahehagh, Baran Parhizkar, Ebrahim Ghaderi, Hozan Loqmani, Behzad Shahmoradi, Ebrahim Mohammadi, Afshin Maleki
Gastric cancer has a high mortality rate in west of Iran. Various environmental elements are proposed as cancer risk factors including trace elements. Trace elements can induce initiation or progression of carcinogenesis via oxidative stress and DNA injury. The aim of this study was to measure and compare some trace element concentration (Ca, Cu, Fe, As, Mg, Ni, Cd and Cr) in gastric cancer tissues and normal tissues. For this purpose, 35 patients with gastric cancer and 30 without any cancer were biopsied...
June 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28633848/novel-synthesis-of-nanocomposite-for-the-extraction-of-sildenafil-citrate-viagra-from-water-and-urine-samples-process-screening-and-optimization
#2
Arash Asfaram, Mehrorang Ghaedi, Mihir Kumar Purkait
A sensitive analytical method is investigated to concentrate and determine trace level of Sildenafil Citrate (SLC) present in water and urine samples. The method is based on a sample treatment using dispersive solid-phase micro-extraction (DSPME) with laboratory-made Mn@ CuS/ZnS nanocomposite loaded on activated carbon (Mn@ CuS/ZnS-NCs-AC) as a sorbent for the target analyte. The efficiency was enhanced by ultrasound-assisted (UA) with dispersive nanocomposite solid-phase micro-extraction (UA-DNSPME). Four significant variables affecting SLC recovery like; pH, eluent volume, sonication time and adsorbent mass were selected by the Plackett-Burman design (PBD) experiments...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633407/exploring-fungus-plant-n-transfer-in-a-tripartite-ant-plant-fungus-mutualism
#3
Céline Leroy, Alain Jauneau, Yves Martinez, Armelle Cabin-Flaman, David Gibouin, Jérôme Orivel, Nathalie Séjalon-Delmas
Background and Aims: The plant Hirtella physophora , the ant Allomerus decemarticulatus and a fungus, Trimmatostroma sp., form a tripartite association. The ants manipulate both the plant trichomes and the fungus to build galleries under the stems of their host plant used to capture prey. In addition to its structural role, the fungus also improves nutrient uptake by the host plant. But it still remains unclear whether the fungus plays an indirect or a direct role in transferring nutrients to the plant...
June 13, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28631293/region-dependent-patterns-of-trabecular-bone-growth-in-the-human-proximal-femur-a-study-of-3d-bone-microarchitecture-from-early-postnatal-to-late-childhood-period
#4
Petar Milovanovic, Danijela Djonic, Michael Hahn, Michael Amling, Björn Busse, Marija Djuric
OBJECTIVES: Parallel with body growth and development, bone structure in non-adults is reorganized to achieve the particular design observed in mature individuals. We traced the changes in three-dimensional trabecular microarchitectural design during the phases of locomotor maturation to clarify how human bone adapts to mechanical demands. MATERIALS AND METHODS: Micro-CT was performed on biomechanically-relevant subregions of the proximal femur (medial, intermediate and lateral neck regions, intertrochanteric region, metaphyseal region) from early postnatal period to late childhood...
June 20, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28631290/a-h2-oxidizing-1-2-3-trichlorobenzene-reducing-multienzyme-complex-isolated-from-the-obligately-organohalide-respiring-bacterium-dehalococcoides-mccartyi-strain-cbdb1
#5
Stefanie Hartwig, Nadya Dragomirova, Anja Kublik, Dominique Türkowsky, Martin von Bergen, Ute Lechner, Lorenz Adrian, R Gary Sawers
Dehalococcoides mccartyi is a small, slow-growing bacterium of the phylum Chloroflexi that conserves energy using aliphatic and aromatic organohalides as electron acceptors, and hydrogen as sole electron donor. A recent study identified a protein complex in the membrane of strain CBDB1 comprising a Hup hydrogenase, a complex iron-sulphur molybdoprotein and a reductive dehalogenase (RdhA) that catalyses reduction of 1,2,3,4-tetrachlorobenzene. Using a combination of size-exclusion chromatography, in-gel hydrogenase activity-staining, immunological analysis and mass spectrometry, we identified here a large molecular mass protein complex solubilized from the cytoplasmic membrane of D...
June 19, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/28628822/spatial-distribution-of-trace-element-ca-normalized-ratios-in-primary-and-permanent-human-tooth-enamel
#6
Théo Tacail, Lenka Kovačiková, Jaroslav Brůžek, Vincent Balter
The trace elements distribution embedded in tooth enamel offers a means to study exposure of toxic metals and allows the reconstruction of dietary behaviors. The quantification of most of the elements with a spatial high resolution (~50μm) is routinely achieved using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). However, the lack of a comprehensive framework of trace elements distribution in enamel jeopardizes any endorsed sampling strategy using LA-ICPMS. The present work is an effort to improve our knowledge on this issue...
June 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28628102/regulation-of-body-weight-and-energy-homeostasis-by-neuronal-cell-adhesion-molecule-1
#7
Thomas Rathjen, Xin Yan, Natalia L Kononenko, Min-Chi Ku, Kun Song, Leiron Ferrarese, Valentina Tarallo, Dmytro Puchkov, Gaga Kochlamazashvili, Sebastian Brachs, Luis Varela, Klara Szigeti-Buck, Chun-Xia Yi, Sonja C Schriever, Sudhir Gopal Tattikota, Anne Sophie Carlo, Mirko Moroni, Jan Siemens, Arnd Heuser, Louise van der Weyden, Andreas L Birkenfeld, Thoralf Niendorf, James F A Poulet, Tamas L Horvath, Matthias H Tschöp, Matthias Heinig, Mirko Trajkovski, Volker Haucke, Matthew N Poy
Susceptibility to obesity is linked to genes regulating neurotransmission, pancreatic beta-cell function and energy homeostasis. Genome-wide association studies have identified associations between body mass index and two loci near cell adhesion molecule 1 (CADM1) and cell adhesion molecule 2 (CADM2), which encode membrane proteins that mediate synaptic assembly. We found that these respective risk variants associate with increased CADM1 and CADM2 expression in the hypothalamus of human subjects. Expression of both genes was elevated in obese mice, and induction of Cadm1 in excitatory neurons facilitated weight gain while exacerbating energy expenditure...
June 19, 2017: Nature Neuroscience
https://www.readbyqxmd.com/read/28627162/fabrication-and-characterization-of-surfaces-modified-with-carboxymethylthio-ligands-for-chelate-assisted-trapping-of-copper
#8
John Onyango Adongo, Tilmann Neubert, Guoguang Sun, Silvia Janietz, Iver Lauermann, Klaus Rademann, Joerg Rappich
Metal ion chelating property was conferred onto silicon (Si) and gold (Au) surfaces by direct electrografting of 4-[(carboxymethyl)thio]benzenediazonium cation (4-CMTBD). Infrared spectroscopic ellipsometry (IRSE) showed presence of characteristic phenyl and carbonyl vibrational bands on the functionalized surfaces as a proof of existence of surface bound organic units of 4-[(carboxymethyl)thio]benzene (4-CMTB). The loss of diazonium group (N≡N+) upon electrografting of 4-CMTBD was investigated using IR spectroscopy...
June 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28627027/elevated-serum-myostatin-level-is-associated-with-worse-survival-in-patients-with-liver-cirrhosis
#9
Hiroki Nishikawa, Hirayuki Enomoto, Akio Ishii, Yoshinori Iwata, Yuho Miyamoto, Noriko Ishii, Yukihisa Yuri, Kunihiro Hasegawa, Chikage Nakano, Takashi Nishimura, Kazunori Yoh, Nobuhiro Aizawa, Yoshiyuki Sakai, Naoto Ikeda, Tomoyuki Takashima, Ryo Takata, Hiroko Iijima, Shuhei Nishiguchi
BACKGROUND: We aimed to elucidate the relationship between serum myostatin levels and other markers including skeletal muscle mass and to investigate the influence of serum myostatin levels on survival for patients with liver cirrhosis (LC). METHODS: A total of 198 LC subjects were analysed in this study. Myostatin levels were measured using stored sera. We retrospectively investigated the relationship between myostatin level and other markers, and the influence of myostatin level on overall survival (OS)...
June 18, 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/28626294/optical-molecular-fluorescence-determination-of-ultra-trace-beryllium-in-occupational-and-environmental-samples-using-highly-alkaline-conditions
#10
Lori Adams, Anoop Agrawal, John P Cronin, Kevin Ashley
Exposures to beryllium (Be), even at extremely low levels, can cause severe health effects in a percentage of those exposed; consequently, occupational exposure limits (OELs) promulgated for this element are the lowest established for any element. This work describes the advantages of using highly alkaline dye solutions for determination of Be in occupational hygiene and environmental samples by means of an optical molecular fluorescence technique after sample extraction in 1-3% (w˖w(-1)) aqueous ammonium bifluoride (NH4HF2)...
2017: International Journal of Environmental Analytical Chemistry
https://www.readbyqxmd.com/read/28621680/associations-between-serum-bilirubin-levels-and-essential-trace-elements-status-in-an-adult-population
#11
You-Fan Peng, Ye-Sheng Wei
OBJECTIVE: This paper aims to evaluate the relations between serum bilirubin and essential trace elements in an adult population. RESULTS: Demographic and clinical data were stratified according to the median of serum bilirubin concentrations (50th percentiles). There were statistical differences in regarding with age, body mass index, white blood count, hemoglobin, mean corpuscular hemoglobin, alanine aminotransferase, creatinine, high-sensitivity C-reactive protein, iron, zinc and copper...
June 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28620727/investigation-of-trace-element-content-in-the-seeds-pulp-and-peel-of-mashui-oranges-using-microwave-digestion-and-icp-ms-analysis
#12
Lingyun Chen, Mei Shen, Ande Ma, Weili Han
Fresh Mashui orange samples were pretreated with microwave digestion using an HNO3-H2O2 system. The levels of Mg, K, Ca, Fe, Mn, Cu, Zn, As, Cd, and Pb in the seeds, pulp, and peel were then determined using inductively coupled plasma mass spectrometry (ICP-MS) combined with collision cell technology (CCT) and kinetic energy discrimination (KED). The standard curve coefficient of determinations of the ten tested elements were between 0.9995 and 0.9999. The instrument detection limit was between 0.112 ng/L and 3...
June 16, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28618663/chemical-characteristics-and-source-apportionment-of-pm2-5-in-lanzhou-china
#13
Jihua Tan, Leiming Zhang, Xueming Zhou, Jingchun Duan, Yan Li, Jingnan Hu, Kebin He
Daily PM2.5 samples were collected during winter 2012 and summer 2013 at an urban site in Lanzhou and were analyzed for chemical compounds including water soluble inorganic ions (WSIN), trace elements, water soluble organic carbon (WSOC), carbonaceous species (OC/EC), polycyclic aromatic hydrocarbons (PAHs), and humic-like substances (HULIS). The seasonal-average reconstructed PM2.5 mass was 120.5μgm(-3) in winter and 34.1μgm(-3) in summer. The top three groups of species in PM2.5 were OC (35.4±13.9μgm(-3)), WSIN (34...
June 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28617866/characterization-of-trace-metal-content-in-the-developing-zebrafish-embryo
#14
Rebecca T Thomason, Michael A Pettiglio, Carolina Herrera, Clara Kao, Jonathan D Gitlin, Thomas B Bartnikas
Trace metals are essential for health but toxic when present in excess. The maintenance of trace metals at physiologic levels reflects both import and export by cells and absorption and excretion by organs. The mechanism by which this maintenance is achieved in vertebrate organisms is incompletely understood. To explore this, we chose zebrafish as our model organism, as they are amenable to both pharmacologic and genetic manipulation and comprise an ideal system for genetic screens and toxicological studies...
2017: PloS One
https://www.readbyqxmd.com/read/28615997/serum-concentration-of-several-trace-metals-and-physical-training
#15
Marcos Maynar, Francisco Llerena, Francisco J Grijota, Javier Alves, María C Robles, Ignacio Bartolomé, Diego Muñoz
BACKGROUND: The aim of this study was to observe the concentrations of trace metals boron, lithium, rubidium, antimony, tin and strontium in the serum of athletes from different modalities and sedentary subjects and the possible influence that different energy sports training modalities can have on their concentration. METHODS: Eighty professional athletes and 31 sedentary males participated in the present survey. All of them were living in Cáceres (Spain). Serum boron, lithium, rubidium, antimony, tin and strontium analysis was performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28615721/spatio-temporal-variability-of-trace-elements-fingerprints-in-cockle-cerastoderma-edule-shells-and-its-relevance-for-tracing-geographic-origin
#16
Fernando Ricardo, Tânia Pimentel, Luciana Génio, Ricardo Calado
Understanding spatio-temporal variability of trace elements fingerprints (TEF) in bivalve shells is paramount to determine the discrimination power of this analytical approach and secure traceability along supply chains. Spatio-temporal variability of TEF was assessed in cockle (Cerastoderma edule) shells using inductively coupled plasma-mass spectrometry (ICP-MS). Four elemental ratios (Mg/Ca, Mn/Ca, Sr/Ca and Ba/Ca) were measured from the shells of specimens originating from eight different ecosystems along the Portuguese coast, as well as from four different areas, within one of them, over two consecutive years (2013 and 2014)...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28609700/identification-of-metabolites-of-vindoline-in-rats-using-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#17
Yuqian Zhang, Yupeng Sun, Xiyan Mu, Lin Yuan, Qiao Wang, Lantong Zhang
Vindoline (VDL) is an indole alkaloid, possessing hypoglycemic and vasodilator effects, and it is also the prodrug of many vinca alkaloids. In this paper, we analyzed in vivo (including plasma, urine, bile and faeces) and in vitro metabolic profile of VDL in rat with ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). The chromatographic separation was performed on a C18 column with a mobile phase consisted of 3mM ammonium acetate buffer and acetonitrile at a flow rate of 300μL/min...
June 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28609106/in-situ-fabrication-of-defective-conx-single-clusters-on-reduced-graphene-oxide-sheets-with-excellent-electrocatalytic-activity-for-oxygen-reduction
#18
Ting He, Xiaojuan Wang, Huiqiong Wu, Hui Xue, Peng Xue, Jing Ma, Minli Tan, Shuanghua He, Rujuan Shen, Lunzhao Yi, Yi Zhang, Juan Xiang
A facile one-step strategy for anchoring defective CoNx single clusters on partly reduced graphene oxide (RGO) is constructed to significantly improve the catalytic performance of non-noble metal complexes toward oxygen reduction reaction (ORR). Sequent loading with trace amounts of metal-free porphyrin and Co2+ in RGO can dramatically enhance both the half-wave potential and peak current density. Intriguingly, the RGO/P/2Co single cluster exhibits the best ORR catalytic performance with the half-wave potential of 0...
June 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28608848/furanoterpene-diversity-and-variability-in-the-marine-sponge-spongia-officinalis-from-untargeted-lc-ms-ms-metabolomic-profiling-to-furanolactam-derivatives
#19
Cléa Bauvais, Natacha Bonneau, Alain Blond, Thierry Pérez, Marie-Lise Bourguet-Kondracki, Séverine Zirah
The Mediterranean marine sponge Spongia officinalis has been reported as a rich source of secondary metabolites and also as a bioindicator of water quality given its capacity to concentrate trace metals. In this study, we evaluated the chemical diversity within 30 S. officinalis samples collected over three years at two sites differentially impacted by anthropogenic pollutants located near Marseille (South of France). Untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomic profiling (C18 LC, ESI-Q-TOF MS) combined with XCMS Online data processing and multivariate statistical analysis revealed 297 peaks assigned to at least 86 compounds...
June 13, 2017: Metabolites
https://www.readbyqxmd.com/read/28604177/evaluation-of-trace-elements-in-potatoes-solanum-tuberosum-from-a-suburban-area-of-naples-italy-the-triangle-of-death
#20
Antonella De Roma, Maria Cesarina Abete, Paola Brizio, Giuseppe Picazio, Marcello Caiazzo, Jacopo Luigi D'auria, Mauro Esposito
Human exposure to contaminated food is a general health concern worldwide; it is necessary to evaluate food safety with respect to contaminants present in the edible parts of major food crops. This study evaluated the concentrations of 17 trace elements (As, Be, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Sb, Se, Sn, Tl, V, and Zn) from 51 potato plantations in the Campania region, inside the area known as the "Triangle of Death," with inductively coupled plasma mass spectrometry analysis. Results confirm that the potatoes collected from the suburban area of Naples contained concentrations of trace elements below the safe limits prescribed by the Food and Agriculture Organization of the United Nations and the World Health Organization...
June 12, 2017: Journal of Food Protection
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