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https://www.readbyqxmd.com/read/28918473/complete-genome-sequences-of-two-insect-specific-flaviviruses
#1
Jermilia Charles, Chandra S Tangudu, Andrew E Firth, Bradley J Blitvich
We determined the complete genomic sequences of two previously discovered insect-specific flaviviruses, Marisma mosquito virus (MMV) and Nanay virus (NANV), using a combination of high-throughput sequencing, reverse transcription-polymerase chain reaction, 5' and 3' rapid amplification of cDNA ends and Sanger sequencing. Complete polyprotein amino acid sequence alignments revealed that the closest known relatives of MMV and NANV are Donggang virus (89% identity, 95% similarity) and Nounané virus (53% identity, 70% similarity), respectively...
September 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28918321/extending-donor-size-in-d-a-%C3%AF-a-organic-dye-for-dye-sensitized-solar-cells-anti-aggregation-and-improving-electron-injection
#2
Yuan-Chun Hu, Wei-Lu Ding, Xing-Liang Peng, Ze-Sheng Li
We have theoretically designed two D-A-π-A dyes 3 and 4 based on the efficient references 1 and 2 by introducing an extra electron donor unit (D2). Via calculating the electronic structures of isolated dyes, we obtain that dyes 3 and 4 possess stronger light-harvesting efficiency imparted by the fluorescence energy transfer of D2 part, maintain comparable lifetime of excited states, and shorten the electron injection time significantly with regard to 1 and 2. Meanwhile, dye 3 positively shifts the edge of virtual states of TiO2 in a larger extent compared to its counterparts...
September 8, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28917710/molecular-identification-of-vermamoeba-vermiformis-from-freshwater-fish-in-lake-taal-philippines
#3
Giovanni D Milanez, Frederick R Masangkay, Rey C Thomas, Ma Olive Grace O Ordona, Gabriel Q Bernales, Vyanna Camille M Corpuz, Hannah Selina V Fortes, Charezze Margarette S Garcia, Larra Camille Nicolas, Veeranoot Nissapatorn
Free Living Amoebae (FLA) are considered ubiquitous. FLAs may infect various biological organisms which act as reservoir hosts. Infected freshwater fishes can pose a public health concern due to possible human consumption. This study aims to identify possible pathogenic FLAs present in freshwater fishes. Seventy five (75) Oreochromis niloticus were studied for the presence of FLAs. Fish organs were suspended in physiologic saline pelleted and cultured in non-nutrient agar (NNA) lawned with Escherichia coli and were incubated in 33 °C for 14 days...
September 13, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28917660/stereotaxic-18-f-fdg-pet-and-mri-templates-with-three-dimensional-digital-atlas-for-statistical-parametric-mapping-analysis-of-tree-shrew-brain
#4
Qi Huang, Binbin Nie, Chen Ma, Jing Wang, Tianhao Zhang, Shaofeng Duan, Shang Wu, Shengxiang Liang, Panlong Li, Hua Liu, Hua Sun, Jiangning Zhou, Lin Xu, Baoci Shan
BACKGROUND: Tree shrews are proposed as an alternative animal model to nonhuman primates due to their close affinity to primates. Neuroimaging techniques are widely used to study brain functions and structures of humans and animals. However, tree shrews are rarely applied in neuroimaging field partly due to the lack of available species specific analysis methods. NEW METHOD: In this study, 10 PET/CT and 10 MRI images of tree shrew brain were used to construct PET and MRI templates; based on histological atlas we reconstructed a three-dimensional digital atlas with 628 structures delineated; then the digital atlas and templates were aligned into a stereotaxic space...
September 13, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28917397/evaluation-of-postoperative-toric-intraocular-lens-alignment-with-anterior-segment-optical-coherence-tomography
#5
Andrea Lucisano, Marco Ferrise, Marco Balestrieri, Massimo Busin, Vincenzo Scorcia
We describe the use of a simple tool to evaluate the postoperative alignment of toric intraocular lenses (IOLs). The entire anterior segment is scanned using anterior segment optical coherence tomography and analyzed with an internal dedicated tool. A topographic map is displayed along with an anterior segment image, including a linear axis marker centered on the corneal apex. The marker can be rotated until it is aligned with the line connecting the IOL marking dots, precisely reproducing the IOL astigmatic axis, which is measured in angle degrees...
August 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28917176/health-based-ingestion-exposure-guidelines-for-vibrio-cholerae-technical-basis-for-water-reuse-applications
#6
Annetta P Watson, Anthony Q Armstrong, George H White, Brandolyn H Thran
U.S. military and allied contingency operations are increasingly occurring in locations with limited, unstable or compromised fresh water supplies. Non-potable graywater reuse is currently under assessment as a viable means to increase mission sustainability while significantly reducing the resources, logistics and attack vulnerabilities posed by transport of fresh water. Development of health-based (non-potable) exposure guidelines for the potential microbial components of graywater would provide a logical and consistent human-health basis for water reuse strategies...
September 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28917035/selgenamic-an-algorithm-for-selenoprotein-gene-assembly
#7
Liang Jiang, Qiong Liu
Computational methods for identifying selenoproteins have been developed rapidly in recent years. However, it is still difficult to identify the open reading frame (ORF) of eukaryotic selenoprotein gene, because the TGA codon for a selenocysteine (Sec) residue in the active center of selenoprotein is traditionally a terminal signal of protein translation. A gene assembly algorithm SelGenAmic has been constructed and presented in this chapter for identifying selenoprotein genes from eukaryotic genomes. A method based on this algorithm was developed to build an optimal TGA-containing-ORF for each TGA in a genome, followed by protein similarity analysis through conserved sequence alignments to screen out selenoprotein genes from these ORFs...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28917034/selenoprofiles-a-computational-pipeline-for-annotation-of-selenoproteins
#8
Didac Santesmasses, Marco Mariotti, Roderic Guigó
Selenoproteins contain selenocysteine (Sec or U), the 21st amino acid, inserted in response to an in-frame UGA codon. UGA normally terminates translation, but in selenoprotein mRNAs it is recoded to specify Sec insertion. For this reason, standard gene prediction programs fail to predict Sec codons, and selenoproteins are usually misannotated in protein databases and genome projects. Selenoprofiles is a computational pipeline able to correctly annotate selenoprotein genes in genomic sequences. This program uses a SECIS-independent approach, based on homology searches, and employs curated built-in profile alignments for all known selenoprotein families...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28916982/chemical-shift-assignments-and-the-secondary-structure-of-the-est3-telomerase-subunit-in-the-yeast-hansenula-polymorpha
#9
Sofia S Mariasina, Sergey V Efimov, Olga A Petrova, Elena V Rodina, Alexander N Malyavko, Maria I Zvereva, Vladimir V Klochkov, Olga A Dontsova, Vladimir I Polshakov
Telomerase is a multisubunit ribonucleoprotein enzyme that is essential for continuous cellular proliferation. A key role of telomerase in cancer and ageing makes it a promising target for the development of cancer therapies and treatments of other age-associated diseases, since telomerase allows unlimited proliferation potential of cells in the majority of cancer types. However, the structure and molecular mechanism of telomerase action are still poorly understood. In budding yeast, telomerase consists of the catalytic subunit, the telomerase reverse transcriptase or Est2 protein, telomerase RNA (TLC1) and two regulatory subunits, Est1 and Est3...
September 15, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28916947/honey-bees-possess-a-polarity-sensitive-magnetoreceptor
#10
Veronika Lambinet, Michael E Hayden, Chloe Reid, Gerhard Gries
Honey bees, Apis mellifera, exploit the geomagnetic field for orientation during foraging and for alignment of their combs within hives. We tested the hypothesis that honey bees sense the polarity of magnetic fields. We created an engineered magnetic anomaly in which the magnetic field generally either converged toward a sugar reward in a watch glass, or away from it. After bees in behavioral field studies had learned to associate this anomaly with a sugar water reward, we subjected them to two experiments performed in random order...
September 15, 2017: Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
https://www.readbyqxmd.com/read/28916838/functionally-distinct-tendons-from-elastin-haploinsufficient-mice-exhibit-mild-stiffening-and-tendon-specific-structural-alteration
#11
Jeremy D Eekhoff, Fei Fang, Lindsey G Kahan, M G Espinosa, Austin J Cocciolone, Jessica E Wagenseil, Robert P Mecham, Spencer P Lake
Elastic fibers are present in low quantities in tendon, where they are located both within fascicles near tenocytes and more broadly in the interfascicular matrix. While elastic fibers have long been known to be significant in the mechanics of elastin-rich tissue (i.e. vasculature, skin, lungs), recent studies have suggested a mechanical role for elastic fibers in tendons that is dependent on specific tendon function. However, the exact contribution of elastin to properties of different types of tendons (e...
September 16, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28916578/nutritional-sustainability-aligning-priorities-in-nutrition-and-public-health-with-agricultural-production
#12
REVIEW
John W Finley, Dennis Dimick, Elizabeth Marshall, Gerald Charles Nelson, Jonathan R Mein, David I Gustafson
Nutrition science-based dietary advice urges changes that may have a great impact on agricultural systems. For example, the 2016 Dietary Guidelines for Americans (DGA) recommends greatly increased fruit and vegetable consumption, but the present domestic production is insufficient to accommodate large-scale adoption of these guidelines. Increasing production to the extent needed to meet the DGA will necessitate changes in an already stressed agriculture and food system and will require nutrition and agriculture professionals to come together in open and collegial discourse...
September 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/28916410/kinetic-stability-and-sequence-structure-studies-of-urine-derived-bence-jones-proteins-from-multiple-myeloma-and-light-chain-amyloidosis-patients
#13
Luis M Blancas-Mejía, Emily B Martin, Angela Williams, Jonathan S Wall, Marina Ramirez-Alvarado
It is now accepted that the ability of a protein to form amyloid fibrils could be associated both kinetic and thermodynamic protein folding parameters. A recent study from our laboratory using recombinant full-length (encompassing the variable and constant domain) immunoglobulin light chains found a strong kinetic control of the protein unfolding for these proteins. In this study, we are extending our analysis by using urine-derived Bence Jones proteins (BJPs) from five patients with light chain (AL) amyloidosis and four patients with multiple myeloma (MM)...
September 1, 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/28916355/grouper-stat1a-is-involved-in-antiviral-immune-response-against-iridovirus-and-nodavirus-infection
#14
Jingcheng Zhang, Xiaohong Huang, Songwei Ni, Jiaxin Liu, Yin Hu, Ying Yang, Yepin Yu, Linli Zhou, Qiwei Qin, Youhua Huang
Signal Transducer and Activator of Transcription 1 (STAT1) has been demonstrated to function as a critical mediator in multiple cell processes, such as cell proliferation, cell death, and innate immune response. Interestingly, two orthologues of human STAT1, including STAT1a and STAT1b genes have been identified in different fish. However, the detailed roles of fish STAT1a in virus replication still remained largely uncertain. Here, we cloned a STAT1a from orange-spotted grouper Epinephelus coioides (EcSTAT1a) and characterized its roles during fish virus infection...
September 12, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28915266/evaluation-of-a-conceptual-framework-for-predicting-navigation-performance-in-virtual-reality
#15
Jascha Grübel, Tyler Thrash, Christoph Hölscher, Victor R Schinazi
Previous research in spatial cognition has often relied on simple spatial tasks in static environments in order to draw inferences regarding navigation performance. These tasks are typically divided into categories (e.g., egocentric or allocentric) that reflect different two-systems theories. Unfortunately, this two-systems approach has been insufficient for reliably predicting navigation performance in virtual reality (VR). In the present experiment, participants were asked to learn and navigate towards goal locations in a virtual city and then perform eight simple spatial tasks in a separate environment...
2017: PloS One
https://www.readbyqxmd.com/read/28914916/continuous-wave-optically-pumped-green-perovskite-vertical-cavity-surface-emitter
#16
Mohd Sharizal Alias, Zhixiong Liu, Abdullah Al-Atawi, Tien Khee Ng, Tom Wu, Boon S Ooi
We report an optically pumped green perovskite vertical-cavity surface-emitter operating in continuous-wave (CW) with a power density threshold of ∼89  kW/cm(2). The device has an active region of CH3NH3PbBr3 embedded in a dielectric microcavity; this feat was achieved with a combination of optimal spectral alignment of the optical cavity modes with the perovskite optical gain, an adequate Q-factor of the microcavity, adequate thermal stability, and improved material quality with a smooth, passivated, and annealed thin active layer...
September 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28914819/construction-of-hierarchical-cuo-cu%C3%A2-o-nico%C3%A2-s%C3%A2-nanowire-arrays-on-copper-foam-for-high-performance-supercapacitor-electrodes
#17
Luoxiao Zhou, Ying He, Congpu Jia, Vladimir Pavlinek, Petr Saha, Qilin Cheng
Hierarchical copper oxide @ ternary nickel cobalt sulfide (CuO/Cu₂O@NiCo₂S₄) core-shell nanowire arrays on Cu foam have been successfully constructed by a facile two-step strategy. Vertically aligned CuO/Cu₂O nanowire arrays are firstly grown on Cu foam by one-step thermal oxidation of Cu foam, followed by electrodeposition of NiCo₂S₄ nanosheets on the surface of CuO/Cu₂O nanowires to form the CuO/Cu₂O@NiCo₂S₄ core-shell nanostructures. Structural and morphological characterizations indicate that the average thickness of the NiCo₂S₄ nanosheets is ~20 nm and the diameter of CuO/Cu₂O core is ~50 nm...
September 15, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28914720/effects-of-corrective-exercise-for-thoracic-hyperkyphosis-on-posture-balance-and-well-being-in-older-women-a-double-blind-group-matched-design
#18
Hyun-Jeong Jang, Lynne C Hughes, Duck-Won Oh, Suhn-Yeop Kim
BACKGROUND AND PURPOSE: The purpose of this study was to identify the effects of a corrective exercise for thoracic hyperkyphosis on posture, balance, and well-being in Korean community-dwelling older women. METHODS: Fifty women 65 years of age and older, recruited from 2 senior centers, participated in this study. Participants were assigned to either the experimental group (EG) or the control group (CG) on the basis of convenience of location, and 22 in each were analyzed...
September 13, 2017: Journal of Geriatric Physical Therapy
https://www.readbyqxmd.com/read/28914663/physicians-decision-making-when-implementing-buprenorphine-with-new-patients-conjoint-analyses-of-data-from-a-cohort-of-current-prescribers
#19
Hannah K Knudsen, Michelle R Lofwall, Sharon L Walsh, Jennifer R Havens, Jamie L Studts
OBJECTIVES: Few studies have considered how providers make decisions to prescribe buprenorphine to new patients with opioid use disorder. This study examined the relative importance of patients' clinical, financial, and social characteristics on physicians' decision-making related to willingness to prescribe buprenorphine to new patients and the number of weeks of medication that they are willing to initially prescribe after induction. METHODS: A national sample of 1174 current prescribers was surveyed...
September 13, 2017: Journal of Addiction Medicine
https://www.readbyqxmd.com/read/28914430/position-paper-recommendations-for-a-digital-mammography-quality-assurance-program%C3%A2-v4-0
#20
J C P Heggie, P Barnes, L Cartwright, J Diffey, J Tse, J Herley, I D McLean, F J Thomson, R K Grewal, L T Collins
In 2001 the ACPSEM published a position paper on quality assurance in screen film mammography which was subsequently adopted as a basis for the quality assurance programs of both the Royal Australian and New Zealand College of Radiologists (RANZCR) and of BreastScreen Australia. Since then the clinical implementation of digital mammography has been realised and it has become evident that existing screen-film protocols were not appropriate to assure the required image quality needed for reliable diagnosis or to address the new dose implications resulting from digital technology...
September 15, 2017: Australasian Physical & Engineering Sciences in Medicine
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