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https://www.readbyqxmd.com/read/28332443/migration-assessment-and-the-threshold-of-toxicological-concern-applied-to-the-safe-design-of-an-acrylic-adhesive-for-food-contact-laminates
#1
Elena Canellas, Paula Vera, Cristina Nerín
Adhesives are widely used in food packaging. The suitability of an acrylic adhesive used on food packaging has been studied. Six potential migrants have been identified using GC-MS and UPLC-QTOF. Five compounds were intentionally added (2-butoxyethanol and 2,4,7,9-tetramethyl-5-decyne-4,7-diol (TMDD) and TMDD ethoxylates). Moreover, one of the compounds identified as 2-(12-(methacryloyloxy)dodecyl)malonic acid was a non-intenionally added substance (NIAS), that could be a methyl metacrylate derivative. This has demonstrated that the identification of compounds in food packaging is an essential step when the risk evaluation of the package is being studied...
March 23, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28317712/lc-esi-qtof-ms-identification-of-novel-antioxidant-peptides-obtained-by-enzymatic-and-microbial-hydrolysis-of-vegetable-proteins
#2
Elena Babini, Davide Tagliazucchi, Serena Martini, Lucilla Dei Più, Andrea Gianotti
Bioactive antioxidant peptides are more and more attracting the attention of food manufacturers for their potential to transform food in functional food, able to prevent a variety of chronic diseases associated with oxidative stress. In the present study proteins extracted from different vegetable sources (KAMUT® khorasan wheat, emmer, lupine and pea) were hydrolyzed with commercial enzymes and Lactobacillus spp. strains. Hydrolysates were separated by size exclusion chromatography and purified fractions were analyzed for their antioxidant activity...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317245/efficient-discovery-of-quality-control-markers-for-gastrodia-elata-tuber-by-fingerprint-efficacy-relationship-modelling
#3
Mingfang Li, Yuan Du, Long Wang, Lilong Jiang, Xiaodong Ma, Ping Zhou, Ping Li, Huijun Li
INTRODUCTION: Gastrodia elata tuber (GET) has been widely used in China as a famous herbal medicine. However, the quality control markers (QCMs) for GET still need further investigation. OBJECTIVE: To develop a rational strategy based on fingerprint-efficacy relationship modelling to discover the efficacy-related QCMs, using GET as a case study. METHODOLOGY: The high-performance liquid chromatography (HPLC) fingerprints of 13 batches of GET extracts were established and 10 common peaks were structurally characterised by HPLC coupled with quadrupole time-of-flight mass spectrometry (QTOF-MS)...
March 20, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28314215/a-metabolomic-approach-shows-sphingosine-1-phosphate-and-lysophospholipids-as-mediators-of-the-therapeutic-effect-of-liver-growth-factor-in-emphysema
#4
A Navarrete, F J Rupérez, T O Mendes, S Pérez-Rial, A Girón-Martínez, R Terrón-Expósito, J J Díaz-Gil, G Peces-Barba, C Barbas, A García
Tobacco smoke exposure is the principal cause of lung tissue destruction, which in turn results in emphysema that leads into shortness of breath. Liver growth factor (LGF, a cell and tissue regenerating factor with therapeutic activity in several organs) has antifibrotic and antioxidant properties that could be useful to promote lung tissue regenerating capacity in damaged lungs. The current study has examined differences in metabolite profiles (fingerprints) of plasma from mice (strain C57BL/6J, susceptible to develop emphysema) exposed to tobacco smoke during six months...
February 27, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28299506/identification-of-the-ligand-of-pru-p-3-a-peach-ltp
#5
Nuria Cubells-Baeza, Cristina Gómez-Casado, Leticia Tordesillas, Carmen Ramírez-Castillejo, María Garrido-Arandia, Pablo González-Melendi, María Herrero, Luis F Pacios, Araceli Díaz-Perales
Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein...
March 15, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28294969/a-metabolomic-approach-for-the-discrimination-of-red-ginseng-root-parts-and-targeted-validation
#6
Gyo In, Hyun Kyu Seo, Hee-Won Park, Kyoung Hwa Jang
Ginsenosides are used as existing markers of red ginseng (RG) quality, and ginsenoside ratios are also indicative of the different components of red ginseng. For the analysis and classification of ginsenoside content, red ginseng was separated into three parts, namely, main roots, lateral roots, and fine roots, and each extract was subjected to ultra-performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-QToF-MS) with multivariate statistical analysis. Principal component analysis (PCA) showed a clear discrimination between the extracts of main roots and fine roots and suggested discrimination markers (four for the main roots and five for the fine roots)...
March 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28290284/uhplc-ms-2-based-approach-for-the-comprehensive-metabolite-profiling-of-bean-vicia-faba-l-by-products-a-promising-source-of-bioactive-constituents
#7
Ibrahim M Abu-Reidah, David Arráez-Román, Ismail Warad, Alberto Fernández-Gutiérrez, Antonio Segura-Carretero
Today, by-products generated from the agro-industrial practices are considered a key source of bioactive and functional components, that can be used for their nutritional and added value properties. New aspects concerning the use of these wastes as by-products in food production additives or supplements with high nutritional and medicinal value have gained substantial interest, due to their possession of economically high-value products. In this sense, the present study describes a thorough characterization of phytochemical compounds from hydro-methanolic extract of broad beans testa by using ultra-high performance liquid chromatography (UHPLC) hyphenated with quadrupole-time-of-flight tandem mass spectrometry (QTOF-MS)...
March 2017: Food Research International
https://www.readbyqxmd.com/read/28287421/in-depth-lipidomic-analysis-of-molecular-species-of-triacylglycerides-diacylglycerides-glycerophospholipids-and-sphingolipids-of-buttermilk-by-gc-ms-fid-hplc-elsd-and-uplc-qtof-ms
#8
Pilar Castro-Gómez, Olimpio Montero, Javier Fontecha
Buttermilk, a byproduct of butter manufacturing, has gained considerable attention due to its high concentration of polar lipids as phospho- and sphingolipids from the milk fat globule membrane (MFGM). These polar lipids (PLs) are essential components of all cellular membranes and exert a variety of indispensable metabolic, neurological, and intracellular signaling processes. Despite its importance, there are few research studies that report a comprehensive characterization of the lipid molecular species of MFGM that could contribute to a better understanding of their putative healthful activities...
March 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28286460/lipid-response-patterns-in-acute-phase-paediatric-plasmodium-falciparum-malaria
#9
Judy Orikiiriza, Izabella Surowiec, Elisabeth Lindquist, Mari Bonde, Jimmy Magambo, Charles Muhinda, Sven Bergström, Johan Trygg, Johan Normark
INTRODUCTION: Several studies have observed serum lipid changes during malaria infection in humans. All of them were focused at analysis of lipoproteins, not specific lipid molecules. The aim of our study was to identify novel patterns of lipid species in malaria infected patients using lipidomics profiling, to enhance diagnosis of malaria and to evaluate biochemical pathways activated during parasite infection. METHODS: Using a multivariate characterization approach, 60 samples were representatively selected, 20 from each category (mild, severe and controls) of the 690 study participants between age of 0...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28285020/uplc-qtof-ms-ms-based-phenolic-profile-and-their-biosynthetic-enzyme-activity-used-to-discriminate-between-cashew-apple-anacardium-occidentale-l-maturation-stages
#10
Aline G Cunha, Edy S Brito, Carlos F H Moura, Paulo R V Ribeiro, Maria Raquel A Miranda
Cashew immature and ripe peduncles (Anacardium occidentale L.) from orange- and red-colored clones CCP 76 and BRS 189, respectively, were prepared as juice or fibrous fraction and submitted to UPLC-MS analyses, while the soluble fraction was also submitted to enzymatic evaluation. Cinnamoyl glucoside was present in ripe juice samples from both cashew clones, while monogalloyl diglucoside and digalloyl glucoside were present in immature juice samples from both cashew clones. Four compounds were found at immature fiber of both clones, anacardic acids (1, 2, 3) and GA19...
February 21, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28284081/an-integrated-strategy-using-uplc-qtof-ms-e-and-uplc-qtof-mrm-enhanced-target-for-pharmacokinetics-study-of-wine-processed-schisandra-chinensis-fructus-in-rats
#11
Kuangyi Liu, Yonggui Song, Yali Liu, Mi Peng, Hanyun Li, Xueliang Li, Bingwei Feng, Pengfei Xu, Dan Su
Currently the pharmacokinetic (PK) research of herbal medicines is still limited and facing critical technical challenges on quantitative analysis of multi-components from biological matrices which often accompanied by lacking of authentic standards and low concentration. This present work contributes to the development of an integrated strategy for extensive pharmacokinetics assessments, and a selective and sensitive method independent of authentic standards for multi-components analysis based on the use of ultra-performance liquid chromatography/quadrupole-time-of-flight/MS(E) (UPLC-TOF-MS(E)) and UPLC-TOF-MRM (rnhanced target)...
February 27, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28277681/hormonemate-derivatives-from-dothiora-sp-an-endophytic-fungus
#12
Mercedes Pérez-Bonilla, Víctor González-Menéndez, Ignacio Pérez-Victoria, Nuria de Pedro, Jesús Martín, Joaquín Molero-Mesa, Manuel Casares-Porcel, María Reyes González-Tejero, Francisca Vicente, Olga Genilloud, José R Tormo, Fernando Reyes
A search for cytotoxic agents from cultures of the endophytic fungus Dothiora sp., isolated from the endemic plant Launaea arborescens, led to the isolation of six new compounds structurally related to hormonemate, with moderate cytotoxic activity against different cancer cell lines. By using a bioassay-guided fractionation approach, hormonemates A-D (1-4), hormonemate (5), and hormonemates E (6) and F (7) were obtained from the acetone extract of this fungus. Their structures were determined using a combination of HRMS, ESI-qTOF-MS/MS, 1D and 2D NMR experiments, and chemical degradation...
March 9, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28273885/iridoids-phenolic-compounds-and-antioxidant-activity-of-edible-honeysuckle-berries-lonicera-caerulea-var-kamtschatica-sevast
#13
Alicja Z Kucharska, Anna Sokół-Łętowska, Jan Oszmiański, Narcyz Piórecki, Izabela Fecka
Iridoid and polyphenol profiles of 30 different honeysuckle berry cultivars and genotypes were studied. Compounds were identified by ultra-performance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-qTOF-MS/MS) in positive and negative ion modes and quantified by HPLC-PDA. The 50 identified compounds included 15 iridoids, 6 anthocyanins, 9 flavonols, 2 flavanonols (dihydroflavonols), 5 flavones, 6 flavan-3-ols, and 7 phenolic acids. 8-epi-Loganic acid, pentosyl-loganic acid, taxifolin 7-O-dihexoside, and taxifolin 7-O-hexoside were identified in honeysuckle berries for the first time...
March 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28268049/application-of-an-esi-qtof-method-for-the-detailed-characterization-of-gsk-3%C3%AE-inhibitors
#14
Angela De Simone, Jessica Fiori, Marina Naldi, Annalisa D'Urzo, Vincenzo Tumiatti, Andrea Milelli, Vincenza Andrisano
The crucial role of Glycogen Synthase Kinase 3 (GSK-3β) as a pivotal player in Alzheimer's Disease (AD) has recently inspired significant attempts to design and synthesize potent kinase inhibitors. In fact GSK-3β is considered the main kinase which catalyzes the microtubule-associated protein tau hyper-phosphorylation and the neurofibrillary tangles (NFT) in vitro and in vivo, The first classes of GSK-3β inhibitors were classified as ATP-competitive and, therefore, they lack of an efficient degree of selectivity over other kinases...
February 27, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28262445/from-untargeted-lc-qtof-analysis-to-characterisation-of-opines-in-abalone-adductor-muscle-theory-meets-practice
#15
Leonie Venter, Peet J Jansen van Rensburg, Du Toit Loots, Andre Vosloo, Jeremie Zander Lindeque
Abalone have a unique ability to use pyruvate, various amino acids and dehydrogenases, to produce opines as means to prevent the accumulation of NADH during anaerobic conditions. In this study, the theoretical masses, formulae and fragment patterns of butylated opines were used to predict which of these compounds could be found in the abalone adductor muscle using untargeted liquid chromatography quadrupole time-of flight-mass spectrometry. These findings were validated using synthesised opine standards. In essence alanopine, lysopine, strombine and tauropine produced in abalone adductor muscle could be characterised using the highest identification confidence levels...
February 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28259866/verification-of-propofol-sulfate-as-a-further-human-propofol-metabolite-using-lc-esi-qqq-ms-and-lc-esi-qtof-ms-analysis
#16
Alexandra Maas, Christoph Maier, Beate Michel-Lauter, Sebastian Broecker, Burkhard Madea, Cornelius Hess
BACKGROUND: Propofol (2,6-diisopropylphenol) is a water-insoluble, intravenous anesthetic that is widely used for the induction and maintenance of anesthesia as well as for endoscopic and pediatric sedation. After admission, propofol undergoes extensive hepatic and extrahepatic metabolism, including direct conjugation to propofol glucuronide and hydroxylation to 2,6-diisopropyl-1,4-quinol. The latter substance subsequently undergoes phase II metabolism, resulting in the formation of further metabolites (1quinolglucuronide, 4quinolglucuronide and 4quinol-sulfate)...
March 1, 2017: Drug Metabolism and Personalized Therapy
https://www.readbyqxmd.com/read/28256553/targeted-mutagenesis-in-the-medicinal-plant-salvia-miltiorrhiza
#17
Bin Li, Guanghong Cui, Guoan Shen, Zhilai Zhan, Luqi Huang, Jiachun Chen, Xiaoquan Qi
CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/Cas9 to precisely knock out the committed diterpene synthase gene (SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a traditional Chinese medicinal herb with significant pharmacological activities, such as vasorelaxation, protection against ischemia-reperfusion injury, and antiarrhythmic effects. Three homozygous and eight chimeric mutants were obtained from 26 independent transgenic hairy root lines by Agrobacterium rhizogenes-mediated transformation...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28254010/preharvest-treatments-with-malic-oxalic-and-acetylsalicylic-acids-affect-the-phenolic-composition-and-antioxidant-capacity-of-coriander-dill-and-parsley
#18
Hussein El-Zaeddi, Ángel Calín-Sánchez, Paulina Nowicka, Juan Martínez-Tomé, Luis Noguera-Artiaga, Francisco Burló, Aneta Wojdyło, Ángel A Carbonell-Barrachina
The effects of a preharvest treatment with malic (MA), oxalic (OA), or acetylsalicylic (ASA) acid at three concentrations (1, 2 and 3mM) on the bioactivity and antioxidant capacity of coriander, dill, and parsley were investigated. The antioxidant capacity of the herbs extracts was assayed by spectrophotometric methods by using three different analytical methods: ORAC, FRAP, and ABTS; the effects of treatments were very positive in coriander, produced intermediate results in dill, and no effects were found in parsley plants...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28252115/improving-sensitivity-and-linear-dynamic-range-of-intact-protein-analysis-using-a-robust-and-easy-to-use-microfluidic-device
#19
Gregory T Roman, James P Murphy
We demonstrate an integrated microfluidic LC device coupled to a QTOF capable of improving sensitivity and linearity for intact protein analysis while also tuning the charge state distributions (CSD) of whole antibodies. The mechanism for sensitivity improvement using microflow ESI is demonstrated by shifting of the CSD to higher charge state, and narrowing of the overall CSD. Both of these aspects serve to improve ion current of the most abundant charge state of antibodies and lead to improvement in sensitivity over high flow ESI by a factor of 15×...
March 2, 2017: Analyst
https://www.readbyqxmd.com/read/28251292/lipidomic-analysis-of-plasma-in-patients-with-lacunar-infarction-using-normal-phase-reversed-phase-two-dimensional-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#20
Li Yang, Pu Lv, Wanpeng Ai, Linnan Li, Sensen Shen, Honggang Nie, Yabing Shan, Yu Bai, Yining Huang, Huwei Liu
Stroke is a major cause of mortality and long-term disability worldwide. The study of biomarkers and pathogenesis is vital for early diagnosis and treatment of stroke. In the present study, a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF/MS) method was employed to measure lipid species in human plasma, including healthy controls and lacunar infarction (LI) patients. As a result, 13 lipid species were demonstrated with significant difference between the two groups, and a "plasma biomarker model" including glucosylceramide (38:2), phosphatidylethanolamine (35:2), free fatty acid (16:1), and triacylglycerol (56:5) was finally established...
March 1, 2017: Analytical and Bioanalytical Chemistry
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