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https://www.readbyqxmd.com/read/29146356/identification-of-cis-trans-isomers-of-menaquinone-7-in-food-as-exemplified-by-dietary-supplements
#1
Arkadiusz Szterk, Adam Zmysłowski, Katarzyna Bus
For the first time, the cis/trans isomer content of menaquinone-7 in food products has been identified and marked. A novel method of marking isomers of vitamin K2MK-7 in dietary supplements was developed and validated. Five different isomers of cis/trans vitamin K2MK-7 were identified. Identification of cis/trans isomers was performed by HRMS-QTOF, whereas their quantities were determined by using CAD and DAD detectors. In the majority of cases, the content of biologically active all trans vitamin K2MK-7 was below its declared content...
March 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29134712/characterization-of-forced-degradation-products-of-canagliflozine-by-lc-qtof-ms-ms-and-in-silico-toxicity-predictions
#2
Shandilya Mahamuni Baira, Pradipbhai D Kalariya, Rakesh Nimbalkar, Prabha Garg, R Srinivas, M V N Kumar Talluri
RATIONALE: Forced degradation studies are useful for better understanding of the stability of active pharmaceutical ingredients and drugs and to generate information about drug degradation pathways and formation of degradation products (DPs). Identification of DPs plays a vital role in establishing the safety and therapeutic benefit of the drug. METHODS: Canagliflozin (CAN) was subjected to different stress conditions as per ICH guidelines (Q1A R2). All the DPs and the drug were well separated on Aquity CSH C18 (100 × 2...
November 14, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29128606/salvianic-acid-a-sodium-protects-huvec-cells-against-tert-butyl-hydroperoxide-induced-oxidative-injury-via-mitochondria-dependent-pathway
#3
Dan Jia, Tian Li, Xiaofei Chen, Xuan Ding, Yifeng Chai, Alex F Chen, Zhenyu Zhu, Chuan Zhang
Salvianic acid A (Danshensu) is a major water-soluble component in Salvia miltiorrhiza (Danshen), which has been widely used in clinic in China for treatment of cardiovascular diseases (CVDs). This study aimed to investigate the protective effects of salvianic acid A sodium (SAAS) against tert-butyl hydroperoxide (t-BHP) induced human umbilical vein endothelial cell (HUVEC) oxidative injury and the potential molecular mechanisms. In the antioxidant activity-assessing models, SAAS significantly prevented t-BHP induced cell proliferation inhibition and apoptosis...
November 8, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29127904/pharmacokinetics-and-brain-uptake-study-of-novel-ampa-receptor-antagonist-perampanel-in-sd-rats-using-a-validated-uhplc-qtof-ms-method
#4
David Paul, Lingesh Allakonda, Amit Sahu, Shruti Surendran, Nanjappan Satheeshkumar
Perampanel (PER) is a novel AMPA receptor antagonist for antiepileptic therapy and is prospective for the treatment of other neurological disorders. A highly sensitive and rapid UHPLC-QTOF-MS method was developed for the quantification of PER in plasma/brain homogenate of SD rat with alogliptin as an internal standard (IS). Chromatographic separation was carried out on an Acquity UPLC HSS Cyano column (100mm×2.1mm, 1.8μm) using gradient mobile phase consisting of 0.1% formic acid and acetonitrile at a flow rate of 0...
November 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29127404/investigation-of-novel-metabolites-potentially-involved-in-the-pathogenesis-of-coronary-heart-disease-using-a-uhplc-qtof-ms-based-metabolomics-approach
#5
Yiping Li, Dong Zhang, Yuan He, Changzhe Chen, Chenxi Song, Yanyan Zhao, Yinxiao Bai, Yang Wang, Jielin Pu, Jingzhou Chen, Yuejin Yang, Kefei Dou
Coronary heart disease (CHD) is associated with complex metabolic disorders, but its molecular aetiology remains unclear. Using a novel nontargeted metabolomics approach, we explored the global metabolic perturbation profile for CHD. Blood samples from 150 patients with severe obstructive CHD and 150 angiographically normal controls were collected. Metabolic fingerprinting was performed by ultra-high performance liquid chromatography coupled to quadruple time-of-flight mass spectrometry (UHPLC-QTOF/MS) technique...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29126433/mosquito-larvicidal-activity-of-cassia-tora-seed-extract-and-its-key-anthraquinones-aurantio-obtusin-and-obtusin
#6
Valentine C Mbatchou, David P Tchouassi, Rita A Dickson, Kofi Annan, Abraham Y Mensah, Isaac K Amponsah, Julia W Jacob, Xavier Cheseto, Solomon Habtemariam, Baldwyn Torto
BACKGROUND: The edible and medicinal leguminous plant Cassia tora L. (Fabaceae) is known to possess insecticidal properties against a wide range of plant-feeding insects. However, the bioactivity of extracts of this plant and their constituents against vectors of medical importance has been largely unexplored. We investigated the mosquito larvicidal activity of the seed extract and its major anthraquinones against larvae of the African malaria vector Anopheles gambiae (s.s.). METHODS: Third-fourth instar larval mortality was observed after 24, 48, 72 and 96 h of exposure to varying doses of the extracts, and two anthraquinones isolates identified using liquid chromatography- quadrupole time of flight mass spectrometry (LC-QtoF-MS)...
November 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29122531/uplc-pda-esi-qtof-ms-profiling-and-potent-anti-hsv-ii-activity-of-eucalyptus-sideroxylon-leaves
#7
Mona M Okba, Rania A El Gedaily, Rehab M Ashour
Eucalyptus is one of the most important and highly exploited genus in family Myrtaceae. An UPLC/PDA/ESI-qTOF-MS method was adopted to identify Eucalyptus sideroxylon Cunn. ex Woolls leaves phytoconstituents. Cytotoxicity of E. sideroxylon leaves phloroglucinol-rich extract (PGRE) on VERO cells was determined. The antiviral effect of PGRE against hepatitis A (HAV), herpes simplex type 1 (HSV-I), herpes simplex type 2 (HSV-II), coxsackie (CoxB4), and adenoviruses was in vitro evaluated using MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)...
November 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29120785/identification-and-characterization-of-pesticide-metabolites-in-brassica-species-by-liquid-chromatography-travelling-wave-ion-mobility-quadrupole-time-of-flight-mass-spectrometry-uplc-twims-qtof-ms
#8
Anna Bauer, Jens Luetjohann, Franziska S Hanschen, Monika Schreiner, Jürgen Kuballa, Eckard Jantzen, Sascha Rohn
A new mass spectrometric method for evaluating metabolite formation of the pesticides thiacloprid, azoxystrobin, and difenoconazole was developed for the Brassica species pak choi and broccoli. Both, distribution and transformation kinetics of the active compounds and their metabolites were analyzed by UPLC-TWIMS-QTOF-MS. Additionally, HR-MS analysis and structure elucidation tools such as diagnostic ions, isotopic matches, and collision cross sections were applied for metabolites identification. Following the application of two plant protection products (containing the above-mentioned active compounds) in a greenhouse study plant material was cryo-milled and extracted with water/methanol...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29109061/extraction-fractionation-and-re-fractionation-of-artemisia-nilagirica-for-anticancer-activity-and-hplc-esi-qtof-ms-ms-determination
#9
Neha Sahu, Sanjeev Meena, Vijaya Shukla, Priyank Chaturvedi, Brijesh Kumar, Dipak Datta, K R Arya
ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants used in traditional medicines are affordable, easily accessible, safer, less toxic and considered as a rich or efficient source of bioactive molecules for modern therapeutics. Artemisia nilagirica (AR) has a long history of use in Indian traditional medicine to combat a wide variety of diseases including cancer. AIM OF THE STUDY: Considering the vast potential of traditional healing plants to deliver safer, less toxic and efficient chemotherapeutics, we have examined anticancer activity of ethanolic extract, bioactive fractions and sub-fractions of AR against different human cancer cell lines along with their phytochemical analysis to understand the insights of novel anticancer activities for further preclinical studies...
November 3, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29100030/plasma-biomarker-discovery-for-early-chronic-kidney-disease-diagnosis-based-on-chemometric-approaches-using-lc-qtof-targeted-metabolomics-data
#10
S Benito, A Sánchez-Ortega, N Unceta, J J Jansen, G Postma, F Andrade, L Aldámiz-Echevarria, L M C Buydens, M A Goicolea, R J Barrio
Chronic kidney disease (CKD) is a progressive pathological condition in which renal function deteriorates in time. The first diagnosis of CKD is often carried out in general care attention by general practitioners by means of serum creatinine (CNN) levels. However, it lacks sensitivity and thus, there is a need for new robust biomarkers to allow the detection of kidney damage particularly in early stages. Multivariate data analysis of plasma concentrations obtained from LC-QTOF targeted metabolomics method may reveal metabolites suspicious of being either up-regulated or down-regulated from urea cycle, arginine methylation and arginine-creatine metabolic pathways in CKD pediatrics and controls...
October 29, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29098829/-difference-of-chemical-constituents-in-eucommiae-cortex-from-different-habitats-by-lc-qtof-ms-ms
#11
Ying Yan, Hui Zhao, Li-Si Zou, Xun-Hong Liu, Chuan Chai, Sheng-Nan Wang, Yu-Jiao Hua
In order to study the influence of ecological environment regarding the synthesis and accumulation of metabolites in Eucommiae Cortex, LC-QTOF MS/MS method combined with multivariate statistical analysis was used to analyze the differences of chemical constituents in Eucommiae Cortex from different habitats. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing...
July 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29080828/effects-of-sulfur-fumigation-on-the-pharmacokinetics-metabolites-and-analgesic-activity-of-radix-paeoniae-alba
#12
Ming Kong, Huan-Huan Liu, Jie Wu, Ming-Qin Shen, Zhi-Gang Wang, Su-Min Duan, Yan-Bo Zhang, He Zhu, Song-Lin Li
ETHNOPHARMACOLOGICAL RELEVANCE: Radix Paeoniae Alba (Baishao, BS), one of the most commonly used traditional Chinese medicinal herbs, has many pharmacological effects including analgesic activity. Previous studies found that sulfur-fumigation, a post-harvest handling process developed to prevent mold contamination of medicinal herbs, altered the quality of BS. However, whether sulfur-fumigation affects the pharmacokinetics, safety and efficacy of BS warrants further investigation. AIM OF THE STUDY: To evaluate the feasibility of sulfur-fumigation as a post-harvest handling process of BS from the viewpoints of pharmacokinetics, safety and efficacy...
October 28, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29072738/correlations-in-the-elemental-and-metabolic-profiles-of-the-lichen-dirinaria-picta-after-road-traffic-exposure
#13
Xulei Huang, Lei Wang, Anna Karen Carrasco Laserna, Sam Fong Yau Li
Lichens can be used as cost-effective biomonitors of elements from road traffic in the urban environment. However, the nature of comprehensive interactions between hazardous heavy metals from road traffic and lichen metabolites remains unclear. In this study, elemental and metabolic profiles of the lichen Dirinaria picta after exposure to road traffic for 3 months in 9 sites of Singapore city were investigated. Concentrations of 34 elements reveal strong correlations among each other and a general increase with traffic exposure level...
November 15, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29064229/validating-quantitative-untargeted-lipidomics-across-nine-liquid-chromatography-high-resolution-mass-spectrometry-platforms
#14
Tomas Cajka, Jennifer T Smilowitz, Oliver Fiehn
Liquid chromatography-mass spectrometry (LC-MS) methods are most often used for untargeted metabolomics and lipidomics. However, methods have not been standardized as accepted "best practice" documents, and reports lack harmonization with respect to quantitative data that enable interstudy comparisons. Researchers use a wide variety of high-resolution mass spectrometers under different operating conditions, and it is unclear if results would yield different biological conclusions depending on the instrument performance...
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29058293/chinese-medicine-formula-shenqi-san-extract-inhibits-proliferation-of-human-lung-adenocarcinoma-a549-cells-via-inducing-apoptosis
#15
Yu Xia, Lu Shi, Zhong-Zhu Ai, De-Zhong Zhang, Yan-Wen Liu, Peng-Tao You
The main purpose of this study was to investigate the active components of the Chinese medicine formula Shenqi San (SS) by high performance liquid chromatography with diode array detector and electrospray ionization-hybrid quadrupole time-of-flight mass spectrum (HPLC-DADESI- QTOF-MS), and demonstrate the anticancer mechanism of SS on human lung adenocarcinoma A549 cells by evaluating the cell proliferation and apoptosis induction. The chloroform extraction of SS (CE-SS) was extracted from SS, while HPLC-DAD-ESI-QTOF-MS assay was performed to identify components of CE-SS...
October 2017: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/29055171/safer-and-healthier-reduced-nitrites-turkey-meat-sausages-using-lyophilized-cystoseira-barbata-seaweed-extract
#16
Sabrine Sellimi, Abdelkarim Benslima, Ghada Ksouda, Veronique Barragan Montero, Mohamed Hajji, Moncef Nasri
Background Nitrite salts are still common additives in the meat industry. The present study provides a first approach on the employment of the lyophilized aqueous extract (WE) of the Tunisian seaweed Cystoseira barbata for the quality enhancement of turkey meat sausage. Methods WE was supplemented as a natural antioxidant agent to investigate its effectiveness in delaying lipid oxidation turkey meat sausages containing reduced amounts of sodium nitrites. Results On storage day 5, all concentrations of WE (0...
October 21, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/29055108/semi-quantitative-analysis-of-tramadol-dextromethorphan-and-metabolites-in-decomposed-skeletal-tissues-by-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-uplc-qtof-ms
#17
H M Cornthwaite, L Labine, J H Watterson
The use of filtration/pass-through extraction (FPTE) and ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-qTOF-MS) to detect tramadol (TRAM), dextromethorphan (DXM) and metabolites from skeletal remains is described. Rats (n=5) received 50 mg/kg tramadol and were euthanized by CO2 asphyxiation approximately 30 min post-dose. Rats (n=4) received 75 mg/kg dextromethorphan and were euthanized by CO2 asphyxiation approximately 45 min post-dose. Remains decomposed to skeleton outdoors and vertebral bones were collected...
October 20, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29054150/pesticides-in-essential-oils-occurrence-and-concentration-in-organic-and-conventional-orange-essential-oils-from-eleven-geographical-origins
#18
Yoann Fillâtre, François-Xavier Gray, Céline Roy
In this paper is described for the first time the application of both gas chromatography-tandem mass spectrometry (GC-MS/MS) and ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-QTOF-MS) to the determination of 75 priority pesticides in orange essential oils (EOs). The sample preparation consisted in a 10-fold dilution of orange EO followed by a direct injection for GC-MS/MS technique and a freeze-out step in the case of UPLC-QTOF-MS analysis. The analytical methods were validated using the SANCO criteria and gave reporting limits (RLs) of 10 μg L(-1), 25 μg L(-1), 50 μg L(-1) and 100 μg L(-1) for 57, 13, 4 and 1 pesticides, respectively...
November 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29052395/-purifying-process-of-gynostemma-pentaphyllum-saponins-based-on-adjoint-marker-online-control-technology-and-identification-of-their-compositions-by-uplc-qtof-ms
#19
Dong-Dong Fan, Yan-Hui Kuang, Li-Hua Dong, Xiao Ye, Liang-Mian Chen, Dong Zhang, Zhen-Shan Ma, Jin-Yu Wang, Jing-Jing Zhu, Zhi-Min Wang, De-Qin Wang, Chu-Yuan Li
To optimize the purification process of gynostemma pentaphyllum saponins (GPS) based on "adjoint marker" online control technology with GPS as the testing index. UPLC-QTOF-MS technology was used for qualitative analysis. "Adjoint marker" online control results showed that the end point of load sample was that the UV absorbance of effluent liquid was equal to half of that of load sample solution, and the absorbance was basically stable when the end point was stable. In UPLC-QTOF-MS qualitative analysis, 16 saponins were identified from GPS, including 13 known gynostemma saponins and 3 new saponins...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29045010/characterization-of-carotenoids-in-rhodothermus-marinus
#20
Emanuel Y C Ron, Merichel Plaza, Thordis Kristjansdottir, Roya R R Sardari, Snaedis H Bjornsdottir, Steinn Gudmundsson, Gudmundur Oli Hreggvidsson, Charlotta Turner, Ed W J van Niel, Eva Nordberg-Karlsson
Rhodothermus marinus, a marine aerobic thermophile, was first isolated from an intertidal hot spring in Iceland. In recent years, the R. marinus strain PRI 493 has been genetically modified, which opens up possibilities for targeted metabolic engineering of the species, such as of the carotenoid biosynthetic pathway. In this study, the carotenoids of the R. marinus type-strain DSM 4252(T) , strain DSM 4253, and strain PRI 493 were characterized. Bioreactor cultivations were used for pressurized liquid extraction and analyzed by ultra-high performance supercritical fluid chromatography with diode array and quadropole time-of-flight mass spectrometry detection (UHPSFC-DAD-QTOF/MS)...
October 17, 2017: MicrobiologyOpen
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