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https://www.readbyqxmd.com/read/28414971/influence-of-sulfur-fumigation-on-the-chemical-profiles-of-atractylodes-macrocephala-koidz-evaluated-by-uflc-qtof-ms-combined-with-multivariate-statistical-analysis
#1
Xue Sun, Xiao-Bing Cui, Hong-Mei Wen, Chen-Xiao Shan, Xin-Zhi Wang, An Kang, Chuan Chai, Wei Li
In the present study, the chemical compositions of Atractylodes macrocephala Koidz. (AMK) were analyzed systematically and influence of sulfur fumigation on the chemical profiles was evaluated by ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UFLC-QTOF-MS) combined with multivariate statistical analysis. 52 components were detected from non-fumigated AMK (NF-AMK) and 28 components were newly produced after sulfur fumigation, out of which 59 major peaks were identified...
March 7, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28407977/structural-characterization-of-reaction-products-of-caftaric-acid-and-bisulfite-present-in-a-commercial-wine-using-high-resolution-mass-spectrometric-and-nuclear-magnetic-resonance-techniques
#2
Yoji Hayasaka, Cory A Black, Jeremy Hack, Paul Smith
Reaction products of bisulfite and caftaric acid were found in wines containing sulfites as a preservative. Acidic compounds were separated from wine and analyzed by HPLC combined with DAD and QTOF mass spectrometer. HPLC chromatograms of the expected [M-H](-) ion and UV absorption revealed the presence of five possible reaction products (a-e). These compounds were isolated then characterized by NMR and confirmed to be the reaction products as follows; 5-sulfo-(E)-caftaric acid (a), 2-sulfo-(Z)-caftaric acid (b), 2-sulfo-(E)-caftaric acid (c), (E)-caftaric acid-4-O-sulfate (d) and (E)-caftaric acid-3-O-sulfate (e)...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28407941/screening-and-identification-of-per-and-polyfluoroalkyl-substances-in-microwave-popcorn-bags
#3
Itsaso Zabaleta, Noelia Negreira, Ekhine Bizkarguenaga, Ailette Prieto, Adrian Covaci, Olatz Zuloaga
Liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC-QToF-MS) was used for the accurate identification (<10ppm) of different polyfluoroalkylphosphates (PAPs) and their intermediate and end degradation products in popcorn bags. Up to 46 per- and polyfluoroalkyl substances (PFASs) and precursors were identified. Moreover, an accurate method based on focused ultrasonic solid-liquid extraction (FUSLE) and a clean-up step with Envi-Carb sorbent was validated and applied to the quantification of 24 PFASs in popcorn bags from over twelve European countries, three American countries and two Asian countries...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28401460/enhanced-production-of-berberine-in-in-vitro-regenerated-cell-of-tinospora-cordifolia-and-its-analysis-through-lcms-qtof
#4
Jitendra Mittal, Madan Mohan Sharma
Tinospora cordifolia is a prioritized medicinal plant and having an immense medicinal importance especially in Indian medicinal system. But this plant needs a regeneration protocol for its rapid propagation. An efficient regeneration protocol was developed for T. cordifolia using nodal explants. High frequency of multiple shoot formation was induced when the nodal segments were cultured on MS medium supplemented with BAP (1.0 mg L(-1)) and 2-iP (0.5 mg L(-1)). The highest mean number of shoots per nodal explant (7...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28400733/optimization-of-huang-lian-jie-du-decoction-for-ischemic-stroke-treatment-and-mechanistic-study-by-metabolomic-profiling-and-network-analysis
#5
Qian Zhang, Junsong Wang, Shanting Liao, Pei Li, Dingqiao Xu, Yan Lv, Minghua Yang, Lingyi Kong
Optimal drug proportions and mechanism deciphering of multicomponent drugs are critical for developing novel therapies to cope with complex diseases, such as stroke. In the present study, orthogonal experimental design was applied to explore the optimal proportion of the four component herbs in Huang-Lian-Jie-Du-Decoction (HLJDD) on the treatment of ischemic stroke. The treatment efficacies and mechanisms were assessed using global and amino acids (AAs) targeted metabolomics, as well as correlation network analysis...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28393524/changes-in-caprine-milk-oligosaccharides-at-different-lactation-stages-analyzed-by-high-performance-liquid-chromatography-coupled-to-mass-spectrometry
#6
Andrea Martín-Ortiz, Daniela Barile, Jaime Salcedo, F Javier Moreno, Alfonso Clemente, Ana I Ruiz-Matute, María L Sanz
Changes of the abundance of caprine milk oligosaccharides (CMO) at different lactation stages have been evaluated by hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-Q MS) and nanoflow liquid chromatography-quadrupole-time-of-flight mass spectrometry (nano-LC-Chip-QTOF MS). Eight major oligosaccharides (OS) were quantified at different lactation stages by HILIC-Q MS, while the use of nano-LC-Chip-QToF MS allowed expanding the study to forty-nine different OS by monitoring neutral non- and fucosylated species, as well as acidic species containing not only N-acetyl-neuraminic acid or N-glycolyl-neuraminic acid residues but also the combination of both sialic acids...
April 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28390941/effects-of-gnaphalium-affine-d-don-on-hyperuricemia-and-acute-gouty-arthritis-anti-hyperuricemia-effect-through-xanthine-oxidase-uricosuric-action-and-anti-inflammatory-activity
#7
Hong-Jian Zhang, Ling-Nan Li, Jie Zhou, Qin-Qin Yang, Pei-Gang Liu, Pan Xu, Wei-Qing Liang, Lin Cheng, Ying-Qi Zhang, Jin-Bao Pu, Yi-Juan Hu
ETHNOPHARMACOLOGICAL RELEVANCE: The Gnaphalium affine D. Don is used in China as a folk medicine to treat gout, anti-inflammatory, antitussive and expectorant activities. The aim of this study was to evaluate the potential of the extract of G. affine to treat hyperuricemia and acute gouty arthritis in animal model. MATERIALS AND METHODS: G. affine extract was evaluated in an experimental model with potassium oxonate (PO) induced hyperuricemia in mice which was used to evaluate anti-hyperuricemia activity and xanthine oxidase (XO) inhibition...
April 5, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28376075/application-value-of-mass-spectrometry-in-the-differentiation-of-benign-and-malignant-liver-tumors
#8
Bo Li, Boan Li, Tongsheng Guo, Zhiqiang Sun, Xiaohan Li, Xiaoxi Li, Han Wang, Weijiao Chen, Peng Chen, Mengran Qiao, Lifang Xia, Yuanli Mao
BACKGROUND Differentiation of malignant from benign liver tumors remains a challenging problem. In recent years, mass spectrometry (MS) technique has emerged as a promising strategy to diagnose a wide range of malignant tumors. The purpose of this study was to establish classification models to distinguish benign and malignant liver tumors and identify the liver cancer-specific peptides by mass spectrometry. MATERIAL AND METHODS In our study, serum samples from 43 patients with malignant liver tumors and 52 patients with benign liver tumors were treated with weak cation-exchange chromatography Magnetic Beads (MB-WCX) kits and analyzed by the Matrix-Assisted Laser Desorption Time of Flight Mass Spectrometry (MALDI-TOF-MS)...
April 4, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28372238/metabolite-profiling-and-volatiles-of-pineapple-wine-and-vinegar-obtained-from-pineapple-waste
#9
Arianna Roda, Luigi Lucini, Fabrizio Torchio, Roberta Dordoni, Dante Marco De Faveri, Milena Lambri
Vinegar is an inexpensive commodity, and economic considerations require that a relatively low-cost raw material be used for its production. An investigation into the use of a new, alternative substrate - pineapple waste - is described. This approach enables the utilization of the pineapple's (Ananas comosus) peels and core, which are usually discarded during the processing or consumption of the fruit. Using physical and enzymatic treatments, the waste was saccharified, and the resulting substrate was fermented with Saccharomyces cerevisiae for 7-10days under aerobic conditions at 25°C...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28372167/a-new-parameter-to-simultaneously-assess-antioxidant-activity-for-multiple-phenolic-compounds-present-in-food-products
#10
Hong Yang, Xuejia Xue, Huan Li, Su Chin Tay-Chan, Seng Poon Ong, Edmund Feng Tian
In this work, we established a new methodology to simultaneously assess the relative reaction rates of multiple antioxidant compounds in one experimental set-up. This new methodology hypothesizes that the competition among antioxidant compounds towards limiting amount of free radical (in this article, DPPH) would reflect their relative reaction rates. In contrast with the conventional detection of DPPH decrease at 515nm on a spectrophotometer, depletion of antioxidant compounds treated by a series of DPPH concentrations was monitored instead using liquid chromatography coupled with quadrupole time-of-flight (LC-QTOF)...
August 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28371476/detection-of-the-recently-emerged-synthetic-cannabinoid-5%C3%A2-f-mdmb-pica-in-legal-high-products-and-human-urine-samples
#11
Lukas Mogler, Florian Franz, Daniel Rentsch, Verena Angerer, Georg Weinfurtner, Mitchell Longworth, Samuel D Banister, Michael Kassiou, Bjoern Moosmann, Volker Auwärter
Indole or indazole based synthetic cannabinoids (SCs) bearing substituents derived from valine or tert-leucine are frequently abused new psychoactive substances (NPS). The emergence of 5 F-MDMB-PICA (methyl N-{[1-(5-fluoropentyl)-1H-indol-3-yl]carbonyl}-3-methylvalinate) on the German drug market is a further example of a substance synthesized in the context of scientific research being misused by clandestine laboratories by adding it to 'legal high' products. In this work we present the detection of 5 F-MDMB-PICA in several 'legal high' products by GC-MS analysis...
March 31, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28370241/lc-q-tof-profile-and-preliminary-stability-studies-of-an-enriched-flavonoid-fraction-of-cecropia-pachystachya-tr%C3%A3-cul-leaves-with-potential-antidepressant-like-activity
#12
Caroline F Ortmann, Helena M Abelaira, Gislaine Z Réus, Zuleide M Ignácio, Vitor Clasen Chaves, Talitha Caldas Dos Santos, Pâmela de Carvalho, Anelise S Carlessi, Livia Bruchchen, Lucineia G Danielski, Simone Gonçalves Cardoso, Angela M de Campos, Fabricia Petronilho, Joyce Rebelo, Meline O S Morais, Francieli Vuolo, Felipe Dal-Pizzol, Emilio L Streck, João Quevedo, Flávio H Reginatto
There is an increasing interest in natural antioxidants that are candidates for prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and is a rich source of polyphenols, especially flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF-Cp) and evaluate the antidepressant-like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters...
March 31, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28369647/developing-a-uhplc-qtof-ms-and-automated-library-search-method-for-screening-drugs-and-toxic-compounds-in-postmortem-specimens
#13
Hsiu-Chuan Liu, Chu-An Yang, Ray H Liu, Dong-Liang Lin
Screening and confirming the presence of drugs and toxic compounds in various matrices are important and challenging tasks routinely faced by forensic and clinical laboratories. Recent advances in the liquid chromatographic and mass spectrometric technologies have provided an opportunity for the development of more specific and effective approaches to achieve the "screening" and "confirmation" goals in a single analytical step. The objectives of this study are: (i) the establishment of an ultra-high performance liquid chromatographic, quadrupole time-of-flight mass spectrometric mass spectrometric and MS-MS spectral database, including 1,200 compounds of interest; and (ii) the development of an effective protocol, using this database and three searching algorithms, for general unknown screening of these compounds...
March 24, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28366571/evaluation-of-direct-analysis-in-real-time-for-the-determination-of-highly-polar-pesticides-in-lettuce-and-celery-using-modified-quick-polar-pesticides-extraction-method
#14
Francisco J Lara, Danny Chan, Michael Dickinson, Antony S Lloyd, Stuart J Adams
Direct analysis in real time (DART) was evaluated for the determination of a number of highly polar pesticides using the Quick Polar Pesticides Extraction (QuPPe) method. DART was hyphenated to high resolution mass spectrometry (HRMS) in order to get the required selectivity that allows the determination of these compounds in complex samples such as lettuce and celery. Experimental parameters such as desorption temperature, scanning speed, and distances between the DART ion source and MS inlet were optimized...
March 15, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28364409/metabolomics-analysis-of-anaphylactoid-reaction-reveals-its-mechanism-in-a-rat-model
#15
Yubin Xu, Na Guo, Deqiang Dou, Xiaoku Ran, Chunyan Liu
BACKGROUND: Anaphylactoid reactions, accounting for more than 77% of all immune-mediated immediate hypersensitivity reactions, have become a serious threat to public health, but their effect mechanism is not clear and diagnostic tests are limited. Comprehensive metabolite analysis may reveal the anaphylactoid effect mechanism systematically and provide reference for future diagnostic purposes. METHODS: Plasma from Brown Norway rats given intravenous injection of saline, compound 48/80 (2...
April 1, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28358117/altered-neo-lacto-series-glycolipid-biosynthesis-impairs-%C3%AE-2-6-sialylation-on-n-glycoproteins-in-ovarian-cancer-cells
#16
Shahidul Alam, Merrina Anugraham, Yen-Lin Huang, Reto S Kohler, Timm Hettich, Katharina Winkelbach, Yasmin Grether, Mónica Núñez López, Nailia Khasbiullina, Nicolai V Bovin, Götz Schlotterbeck, Francis Jacob
The (neo-) lacto series glycosphingolipids (nsGSLs) comprise of glycan epitopes that are present as blood group antigens, act as primary receptors for human pathogens and are also increasingly associated with malignant diseases. Beta-1, 3-N-acetyl-glucosaminyl-transferase 5 (B3GNT5) is suggested as the key glycosyltransferase for the biosynthesis of nsGSLs. In this study, we investigated the impact of CRISPR-Cas9 -mediated gene disruption of B3GNT5 (∆B3GNT5) on the expression of glycosphingolipids and N-glycoproteins by utilizing immunostaining and glycomics-based PGC-UHPLC-ESI-QTOF-MS/MS profiling...
March 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28348510/comprehensive-optimization-of-lc-ms-metabolomics-methods-using-design-of-experiments-colmed
#17
Seth D Rhoades, Aalim M Weljie
INTRODUCTION: Both reverse-phase and HILIC chemistries are deployed for liquid-chromatography mass spectrometry (LC-MS) metabolomics analyses, however HILIC methods lag behind reverse-phase methods in reproducibility and versatility. Comprehensive metabolomics analysis is additionally complicated by the physiochemical diversity of metabolites and array of tunable analytical parameters. OBJECTIVE: Our aim was to rationally and efficiently design complementary HILIC-based polar metabolomics methods on multiple instruments using Design of Experiments (DoE)...
December 2016: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/28347564/the-gut-microbiota-metabolism-of-pomegranate-or-walnut-ellagitannins-yields-two-urolithin-metabotypes-that-correlate-with-cardiometabolic-risk-biomarkers-comparison-between-normoweight-overweight-obesity-and-metabolic-syndrome
#18
María V Selma, Antonio González-Sarrías, Jordi Salas-Salvadó, Cristina Andrés-Lacueva, Cesarettin Alasalvar, Asım Örem, Francisco A Tomás-Barberán, Juan C Espín
BACKGROUND & AIMS: Urolithins are microbial metabolites produced after consumption of ellagitannin-containing foods such as pomegranates and walnuts. Parallel to isoflavone-metabolizing phenotypes, ellagitannin-metabolizing phenotypes (urolithin metabotypes A, B and 0; UM-A, UM-B and UM-0, respectively) can vary among individuals depending on their body mass index (BMI), but correlations between urolithin metabotypes (UMs) and cardiometabolic risk (CMR) factors are unexplored. We investigated the association between UMs and CMR factors in individuals with different BMI and health status...
March 16, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28343060/uv-chlorine-treatment-of-carbamazepine-transformation-products-and-their-formation-kinetics
#19
Yanheng Pan, ShuangShuang Cheng, Xin Yang, Jingyue Ren, Jingyun Fang, Chii Shang, Weihua Song, Lushi Lian, Xinran Zhang
Carbamazepine (CBZ) is one of the pharmaceuticals most frequently detected in the aqueous environment. This study investigated the transformation products when CBZ is degraded by chlorine under ultraviolet (UV) irradiation (the UV/chlorine process). Detailed pathways for the degradation of CBZ were elucidated using ultra-high performance liquid chromatography (UHPLC)-quadrupole time-of-flight mass spectrometry (QTOF-MS). CBZ is readily degraded by hydroxyl radicals (HO) and chlorine radicals (Cl) in the UV/chlorine process, and 24 transformation products were identified...
March 23, 2017: Water Research
https://www.readbyqxmd.com/read/28342305/metabolic-profiling-of-nuciferine-in-rat-urine-plasma-bile-and-feces-after-oral-administration-using-ultra-high-performance-liquid-chromatography-diode-array-detection-quadrupole-time-of-flight-mass-spectrometry
#20
Xiao-Lei Wu, Ming-Jiang Wu, Xin-Ze Chen, Hao-Ling Ma, Li-Qin Ding, Feng Qiu, Qin Pan, De-Qin Zhang
Nuciferine, a major alkaloid found in Nelumbinis Folium, exhibits a broad spectrum of bioactivities, such as antiobesity, anti-diabetes and anti-inflammatory. However, many research regarding nuciferine focused on the extraction, isolation and biological activity, the metabolism is not comprehensively explained in vivo. Thence, the present of this paper is to establish a simple method for speculating metabolites of nuciferine. A total of 15 metabolites were detected and tentatively identified through ultra high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry (UHPLC-DAD-QTOF-MS), including 7 new metabolites...
March 18, 2017: Journal of Pharmaceutical and Biomedical Analysis
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