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https://www.readbyqxmd.com/read/27926813/perturbation-of-h3k27me3-associated-epigenetic-processes-increases-agrobacterium-mediated-transformation
#1
Hidekazu Iwakawa, Benjamin C Carter, Brett C Bishop, Joe Ogas, Stanton B Gelvin
Agrobacterium-mediated transformation is a core technology for basic plant science and agricultural biotechnology. Improving transformation frequency is a major goal for plant transgenesis. We previously showed that T-DNA insertions in some histone genes decreased transformation susceptibility, whereas overexpression of several Arabidopsis H2A and H4 isoforms increased transformation. Overexpression of several histone H2B and H3 isoforms had little effect on transformation frequency. However, overexpression of histone H3-11 (HTR11) enhanced transformation...
December 7, 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/27926684/what-s-new-in-orthopaedic-basic-science
#2
Matthew J Allen
No abstract text is available yet for this article.
December 7, 2016: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/27925188/a-human-3d-in-vitro-model-to-assess-the-relationship-between-osteoporosis-and-dissemination-to-bone-of-breast-cancer-tumour-cells
#3
F Salamanna, V Borsari, S Brogini, P Torricelli, S Cepollaro, M Cadossi, M Fini
Despite consistent improvements in diagnostic and therapeutic strategies for breast cancer, up to 40% of patients will develop bone metastases. To reduce the morbidity and complications related with bone metastases, it is imperative to reduce their etiological factors. Osteoporosis, being characterized by a sudden estrogen deficiency, may provide a favorable condition for bone metastasis. This work, using a humanized 3D in vitro model, aims at evaluating the relationship between osteoporosis and breast cancer-derived bone metastases...
December 7, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27919479/how-to-refer-to-people-with-disease-in-research-outputs-the-disconnection-between-academic-practise-and-that-preferred-by-people-with-multiple-sclerosis
#4
David Baker, Ananthi Anandhakrishnan, Katie A Tuite-Dalton, Hazel Lockart-Jones, Rodden M Middleton, David V Ford, Christina Crowe, Gavin Giovannoni
BACKGROUND: Increasingly, Government and Charity funders require public engagement in research. Invariably these research outputs describe the condition of someone with the disease of interest. We therefore sought to identify the preferred descriptor of someone with a disease, such as multiple sclerosis (MS) and to determine what descriptors are currently used by academics. METHODS: Several surveys were undertaken: one from the Research Network of the MS Society (MSSRN), a major MS Charity within the United Kingdom, who are involved in reviewing grant applications, priority setting and research governance (n=146), and surveys from both the United Kingdom MS register (MSR; n=1713) and the North American Research Committee on Multiple Sclerosis (NARCOMS) registry (n=518)...
November 2016: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/27916427/tea-cocoa-coffee-and-affective-disorders-vicious-or-virtuous-cycle
#5
REVIEW
Tatiana García-Blanco, Alberto Dávalos, Francesco Visioli
BACKGROUND: The prevalence of psychiatric disorders is increasing worldwide, which underscores the importance of increasing research in this field, in terms of better detection, prevention based on improvement of lifestyle and diet, and effectiveness of treatment. Increasing evidence suggest that diet and exercise can affect proper neuronal development and physiology and protect the brain from neurological illnesses or injuries. Of note, cocoa, tea, and coffee are being actively investigated because they are rich in (poly)phenolic compounds that can modulate mental health, namely brain plasticity, behavior, mood, depression, and cognition...
November 25, 2016: Journal of Affective Disorders
https://www.readbyqxmd.com/read/27915165/re-assessing-gallium-67-as-a-therapeutic-radionuclide
#6
Muhamad F Bin Othman, Nabil R Mitry, Valerie J Lewington, Philip J Blower, Samantha Y A Terry
INTRODUCTION: Despite its desirable half-life and low energy Auger electrons that travel further than for other radionuclides, (67)Ga has been neglected as a therapeutic radionuclide. Here, (67)Ga is compared with Auger electron emitter (111)In as a potential therapeutic radionuclide. METHODS: Plasmid pBR322 studies allowed direct comparison between (67)Ga and (111)In (1MBq) in causing DNA damage, including the effect of chelators (EDTA and DTPA) and the effects of a free radical scavenger (DMSO)...
October 29, 2016: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/27914452/inflammatory-immune-infiltration-in-human-tumors-role-in-pathogenesis-and-prognostic-and-diagnostic-value
#7
REVIEW
A V Bogolyubova, P V Belousov
The cellular microenvironment directly and indirectly influences tumor development and possesses prognostic and in some cases diagnostic value. Over the years, understanding of structural organization of the immune/inflammatory moiety of neoplasms as well as in-depth phenotypic and transcriptomic profiling of its cellular components together provide more and more insights in both basic and translational medical science. In this review, we will discuss the specific roles of various stromal cells and their impact on neoplastic progression as well as address the use of quantitative and phenotypic analysis of immune/inflammatory infiltrate for diagnostics and predicting the clinical course of human malignancies...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27914089/generating-and-purifying-fab-fragments-from-human-and-mouse-igg-using-the-bacterial-enzymes-ides-speb-and-kgp
#8
Jonathan Sjögren, Linda Andersson, Malin Mejàre, Fredrik Olsson
Fab fragments are valuable research tools in various areas of science including applications in imaging, binding studies, removal of Fc-mediated effector functions, mass spectrometry, infection biology, and many others. The enzymatic tools for the generation of Fab fragments have been discovered through basic research within the field of molecular bacterial pathogenesis. Today, these enzymes are widely applied as research tools and in this chapter, we describe methodologies based on bacterial enzymes to generate Fab fragments from both human and mouse IgG...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27914044/the-framework-of-computational-protein-design
#9
Ilan Samish
Computational protein design (CPD) has established itself as a leading field in basic and applied science with a strong coupling between the two. Proteins are computationally designed from the level of amino acids to the level of a functional protein complex. Design targets range from increased thermo- (or other) stability to specific requested reactions such as protein-protein binding, enzymatic reactions, or nanotechnology applications. The design scheme may encompass small regions of the proteins or the entire protein...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27913797/promoting-gait-recovery-and-limiting-neuropathic-pain-after-spinal-cord-injury-two-sides-of-the-same-coin
#10
Catherine Mercier, Meyke Roosink, Jason Bouffard, Laurent J Bouyer
Most persons living with a spinal cord injury experience neuropathic pain in the months following their lesion, at the moment where they receive intensive gait rehabilitation. Based on studies using animal models, it has been proposed that central sensitization in nociceptive pathways (maladaptive plasticity) and plasticity related to motor learning (adaptive plasticity) share common neural mechanisms and compete with each other. This article aims to address the discrepancy between the growing body of basic science literature supporting this hypothesis and the general belief in rehabilitation research that pain and gait rehabilitation represent two independent problems...
December 2, 2016: Neurorehabilitation and Neural Repair
https://www.readbyqxmd.com/read/27913313/the-role-of-vitamin-d-and-vdr-in-carcinogenesis-through-epidemiology-and-basic-sciences
#11
REVIEW
Borja Bandera Merchan, Sonsoles Morcillo, Gracias Martin-Nuñez, Francisco José Tinahones, Manuel Macías-González
In the last two decades vitamin D (VD) research has demonstrated new extraskeletal actions of this pre-hormone, suggesting a protective role of this secosteroid in the onset, progression and prognosis of several chronic noncommunicable diseases, such as cardiovascular disease, diabetes mellitus or cancer. Regarding carcinogenesis, both preclinical and epidemiological evidence available show oncoprotective actions of VD and its receptor, the VDR. However, in late neoplastic stages the VD system (VDS) seems to be less functional, which appears to be due to an epigenetic silencing of the system...
November 29, 2016: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27912812/endodontic-microbiology-and-pathobiology-current-state-of-knowledge
#12
REVIEW
Ashraf F Fouad
Newer research tools and basic science knowledge base have allowed the exploration of endodontic diseases in the pulp and periapical tissues in novel ways. The use of next generation sequencing, bioinformatics analyses, genome-wide association studies, to name just a few of these innovations, has allowed the identification of hundreds of microorganisms and of host response factors. This review addresses recent advances in endodontic microbiology and the host response and discusses the potential for future innovations in this area...
January 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/27909796/-clinical-morphological-and-molecular-biological-characteristics-of-the-aging-eye
#13
M R R Böhm, H Thomasen, F Parnitzke, K-P Steuhl
BACKGROUND: The physiological aging of the eye is associated with loss of visual function. Age-related changes of the eye can result in ophthalmological diseases. The aim of this article is to display morphological, histological and molecular biological alterations of the aging eye. MATERIAL AND METHODS: A web-based search and review of the literature for aging of the visual system including cornea, lens, vitreous humor, retina, retinal pigment epithelium (RPE), choroidea and optic nerve were carried out...
December 1, 2016: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/27909612/nonsurgical-treatment-options-in-peyronie-s-disease-2016-update
#14
REVIEW
Raidh A Talib, Mohammed Abdulkareem Ibrahim, Önder Cangüven
Peyronie's disease (PD) is an inflammatory condition of penile tunica albuginea which commonly ends with penile curvature and difficulty in vaginal penetration. Unfortunately, the pathophysiology of PD has not been completely understood. In this paper, we will review what is known about the pathophysiology of PD and the nonsurgical medical treatment options that have been trialed as a result. In the last 5 years, commonly used oral medications left their places to intralesional therapies. Clostridium collagenase, which is the only Food and Drug Administration (FDA) approved treatment for PD, is now the most prescribed intralesional therapy in the last years...
December 2016: Turkish Journal of Urology
https://www.readbyqxmd.com/read/27909510/interactive-in-vitro-training-in-physics-of-radiofrequency-ablation-for-physicians-and-medical-engineering-students
#15
REVIEW
Haber T, Kleister G, Selman B, Härtig J, Melichercik J, Ismer B
Radiofrequency (RF) ablation requires a complex set of devices as well as profound electrophysiological experience and substantial knowledge of physical science basics. To establish RF ablation in-vitro teaching-system, six workstations were equipped with computer-controlled RF ablation generators. Universal connection boxes allow ablation-essays with catheters of different make and model. Special wetlabs were developed combining a basin containing isotonic saline solution with a thermostat and a pump to simulate blood flow...
June 2016: Journal of Atrial Fibrillation
https://www.readbyqxmd.com/read/27909075/cell-scientist-to-watch-brian-stramer
#16
(no author information available yet)
Brian Stramer completed his undergraduate degree at the University of Rochester in Rochester, NY, and then moved to pursue his PhD at Tufts University in Boston, MA. He then crossed the Atlantic to work in the laboratory of Paul Martin as a US/UK Royal Society Fellow at the University of Bristol, UK. Before becoming a lecturer and a group leader at King's College London in 2008, Brian was also a lecturer of Veterinary Basic Sciences at the Royal Veterinary College in London. In 2015, Brian received a Wellcome Trust Investigator Award, which he will use to continue his research on the regulation of contact inhibition of locomotion and its roles in animal physiology...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27908839/the-transition-of-acute-postoperative-pain-to-chronic-pain-an-integrative-overview-of-research-on-mechanisms
#17
C Richard Chapman, Charles J Vierck
The nature of the transition from acute to chronic pain still eludes explanation, but chronic pain resulting from surgery provides a natural experiment that invites both clinical epidemiological investigation and basic scientific inquiry into the mechanisms of this transition. The primary purpose of this paper is to review current knowledge and hypotheses on the transition from acute to persistent postsurgical pain, summarizing literature on clinical epidemiological studies of persistent postsurgical pain development, as well as basic neurophysiological studies targeting mechanisms in the periphery, spinal cord, and brain...
November 28, 2016: Journal of Pain: Official Journal of the American Pain Society
https://www.readbyqxmd.com/read/27907981/use-of-social-media-in-education-among-medical-students-in-saudi-arabia
#18
Anas Khaleel Alsuraihi, Ahmed Saeed Almaqati, Sultan Adnan Abughanim, Nisreen Abdulrahman Jastaniah
PURPOSE: Social media (SM), a virtual place where people can share, exchange, and communicate their ideas and knowledge, has become the new trend in communication and learning. This study aims to explore Saudi Arabian medical students' usage of SM and to discover the most common resources used in medical education. Furthermore, it aims to illustrate students' belief about the influence of SM on their learning. METHODS: This cross-sectional study administered validated questionnaires to medical students from different universities in Saudi Arabia, via emails, Twitter, Facebook, and short message service...
December 2016: Korean Journal of Medical Education
https://www.readbyqxmd.com/read/27907110/electrospray-ionization-efficiency-is-dependent-on-different-molecular-descriptors-with-respect-to-solvent-ph-and-instrumental-configuration
#19
Andreas Kiontke, Ariana Oliveira-Birkmeier, Andreas Opitz, Claudia Birkemeyer
Over the past decades, electrospray ionization for mass spectrometry (ESI-MS) has become one of the most commonly employed techniques in analytical chemistry, mainly due to its broad applicability to polar and semipolar compounds and the superior selectivity which is achieved in combination with high resolution separation techniques. However, responsiveness of an analytical method also determines its suitability for the quantitation of chemical compounds; and in electrospray ionization for mass spectrometry, it can vary significantly among different analytes with identical solution concentrations...
2016: PloS One
https://www.readbyqxmd.com/read/27906626/advances-in-purine-and-pyrimidine-metabolism-in-health-and-diseases
#20
Michio Hirano, Godefridus J Peters
In June, 2015, the Purine and Pyrimidine Society organized the 16th biennial symposium on Purine and Pyrimidine metabolism at the Faculty House of Columbia University, New York City. This exciting meeting focused on these important molecules, new developments in inborn errors of metabolism; therapeutic analogs. In addition, the biochemistry of mammalian and non-mammalian systems were discussed. Due to significant advances in molecular medicine, the boundaries between clinical and basic sciences have merged into exciting translational research, of which a small portion was highlighted in the presymposium...
December 2016: Nucleosides, Nucleotides & Nucleic Acids
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