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https://www.readbyqxmd.com/read/28231413/metabolic-chemical-reporters-of-glycans-exhibit-cell-type-selective-metabolism-and-glycoprotein-labeling
#1
Anna R Batt, Balyn W Zaro, Marisol X Navarro, Matthew Robert Pratt
Since the pioneering work by Reutter and co-workers that demonstrated structural flexibility in the carbohydrate biosynthesis and glycosylation pathways, many different labs have used unnatural monosaccharide analogs to perform glycan engineering on the surface of living cells. A subset of these unnatural monosaccharides contain bioorthogonal groups that enable the selective installation of visualization or enrichment tags. These metabolic chemical reporters (MCRs) have proven to be powerful for the unbiased identification of glycoproteins; however they do have certain limitations...
February 23, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28231306/effects-of-adamts14-genetic-polymorphism-and-cigarette-smoking-on-the-clinicopathologic-development-of-hepatocellular-carcinoma
#2
Ming-Jen Sheu, Ming-Ju Hsieh, Ying-Erh Chou, Po-Hui Wang, Chao-Bin Yeh, Shun-Fa Yang, Hsiang-Lin Lee, Yu-Fan Liu
BACKGROUND: ADAMTS14 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs), which are proteolytic enzymes with a variety of further ancillary domain in the C-terminal region for substrate specificity and enzyme localization via extracellular matrix association. However, whether ADAMTS14 genetic variants play a role in hepatocellular carcinoma (HCC) susceptibility remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: Four non-synonymous single-nucleotide polymorphisms (nsSNPs) of the ADAMTS14 gene were examined from 680 controls and 340 patients with HCC...
2017: PloS One
https://www.readbyqxmd.com/read/28230982/synthesis-of-%C3%AE-1-2-linked-6-deoxy-l-altropyranose-oligosaccharides-via-gold-i-catalyzed-glycosylation-of-a-ortho-hexynylbenzoate-donor
#3
Zhengnan Shen, Hani Mobarak, Wei Li, Göran Widmalm, Biao Yu
The β-(1-2)-linked 6-deoxy-L-altropyranose di- to penta-saccharides 2-5, relevant to the O-antigen of the infectious Yersinia enterocolitica O:3, were synthesized for the first time. The challenging 1,2-cis-altropyranosyl linkage was assembled effectively via glycosylation with 2-O-benzyl-3,4-di-O-benzoyl-6-deoxy-L-altropyranosyl ortho-hexynylbenzoate (7) under the catalysis of PPh3AuNTf2. NMR and molecular modeling studies showed that the pentasaccharide (5) adopted a left-handed helical conformation.
February 23, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28230459/estimated-cost-effectiveness-cost-benefit-and-risk-reduction-associated-with-an-endocrinologist-pharmacist-diabetes-intense-medical-management-tune-up-clinic
#4
Jan D Hirsch, Mark Bounthavong, Anisa Arjmand, David R Ha, Christine L Cadiz, Andrew Zimmerman, Heather Ourth, Anthony P Morreale, Steven V Edelman, Candis M Morello
BACKGROUND: In 2012 U.S. diabetes costs were estimated to be $245 billion, with $176 billion related to direct diabetes treatment and associated complications. Although a few studies have reported positive glycemic and economic benefits for diabetes patients treated under primary care physician (PCP)-pharmacist collaborative practice models, no studies have evaluated the cost-effectiveness of an endocrinologist-pharmacist collaborative practice model treating complex diabetes patients versus usual PCP care for similar patients...
March 2017: Journal of Managed Care & Specialty Pharmacy
https://www.readbyqxmd.com/read/28230186/glycan-independent-binding-and-internalization-of-human-igm-to-fcmr-its-cognate-cellular-receptor
#5
Katy A Lloyd, Jiabin Wang, Britta C Urban, Daniel M Czajkowsky, Richard J Pleass
IgM is the first antibody to be produced in immune responses and plays an important role in the neutralization of bacteria and viruses. Human IgM is heavily glycosylated, featuring five N-linked glycan sites on the μ chain and one on the J-chain. Glycosylation of IgG is known to modulate the effector functions of Fcγ receptors. In contrast, little is known about the effect of glycosylation on IgM binding to the human Fcμ receptor (hFCMR). In this study, we identify the Cμ4 domain of IgM as the target of hFCMR, and show that binding and internalization of IgM by hFCMR is glycan-independent...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229927/alteration-of-n-glycan-expression-profile-and-glycan-pattern-of-glycoproteins-in-human-hepatoma-cells-after-hcv-infection
#6
Tian Xiang, Ganglong Yang, Xiaoyu Liu, Yidan Zhou, Zhongxiao Fu, Fangfang Lu, Jianguo Gu, Naoyuki Taniguchi, Zengqi Tan, Xi Chen, Yan Xie, Feng Guan, Xiao-Lian Zhang
BACKGROUND: Hepatitis C virus (HCV) infection causes chronic liver diseases, liver fibrosis and even hepatocellular carcinoma (HCC). However little is known about any information of N-glycan pattern in human liver cell after HCV infection. METHODS: The altered profile of N-glycans in HCV-infected Huh7.5.1 cell were analyzed by using mass spectrometry. Then, lectin microarray, lectin pull-down assay, reverse transcription-quantitative real time PCR (RT-qPCR) and western-blotting were used to identify the altered N-glycosylated proteins and glycosyltransferases...
February 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28229385/early-pregnancy-biochemical-predictors-of-gestational-diabetes-mellitus
#7
REVIEW
Camille E Powe
PURPOSE OF REVIEW: Universal oral glucose tolerance-based screening is employed to identify pregnant women with gestational diabetes mellitus (GDM), as treatment of this condition decreases the risk of associated complications. A simple and accurate blood test which identifies women at low or high risk for GDM in the first trimester would have the potential to decrease costs and improve outcomes through prevention or treatment. This review summarizes published data on early pregnancy biomarkers which have been tested as predictors of GDM...
February 2017: Current Diabetes Reports
https://www.readbyqxmd.com/read/28228947/a-randomized-clinical-trial-of-diabetes-self-management-for-mexican-americans-are-there-serendipitous-health-benefits-for-supporters-of-study-participants
#8
Sharon A Brown, Alexandra A García, Philip R Orlander, Craig L Hanis
OBJECTIVES: Studies of social support in diabetes have focused on the effects of support on the person with type 2 diabetes. We explored diabetes prevention effects of a culturally tailored diabetes self-management intervention in individuals without diabetes who were supporters of intervention participants. METHODS: This is a secondary analysis of data from a randomized clinical trial that involved 256 Mexican Americans with diabetes. Each study participant designated a supporter-spouse, relative, friend-who attended intervention sessions and assisted participants in attaining effective diabetes self-management...
2017: SAGE Open Medicine
https://www.readbyqxmd.com/read/28228925/what-can-mathematical-modelling-say-about-cho-metabolism-and-protein-glycosylation
#9
REVIEW
Sarah N Galleguillos, David Ruckerbauer, Matthias P Gerstl, Nicole Borth, Michael Hanscho, Jürgen Zanghellini
Chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins. A deep, quantitative understanding of CHO metabolism and mechanisms involved in protein glycosylation has proven to be attainable through the development of high throughput technologies. Here we review the most notable accomplishments in the field of modelling CHO metabolism and protein glycosylation.
2017: Computational and Structural Biotechnology Journal
https://www.readbyqxmd.com/read/28228850/silyl-protective-groups-influencing-the-reactivity-and-selectivity-in-glycosylations
#10
REVIEW
Mikael Bols, Christian Marcus Pedersen
Silyl groups such as TBDPS, TBDMS, TIPS or TMS are well-known and widely used alcohol protective groups in organic chemistry. Cyclic silylene protective groups are also becoming increasingly popular. In carbohydrate chemistry silyl protective groups have frequently been used primarily as an orthogonal protective group to the more commonly used acyl and benzyl protective groups. However, silyl protective groups have significantly different electronic and steric requirements than acyl and alkyl protective groups, which particularly becomes important when two or more neighboring alcohols are silyl protected...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28228555/microrna-15b-suppresses-th17-differentiation-and-is-associated-with-pathogenesis-of-multiple-sclerosis-by-targeting-o-glcnac-transferase
#11
Ruiqiong Liu, Xiaofeng Ma, Li Chen, Yang Yang, Yi Zeng, Jie Gao, Wei Jiang, Fang Zhang, Daojing Li, Bin Han, Ranran Han, Rongfang Qiu, Wei Huang, Yan Wang, Junwei Hao
IL-17-producing Th17 cells have gradually become considered as key factors in the pathogenesis of many autoimmune diseases, including multiple sclerosis (MS). Although the involvement of certain microRNAs in the development of MS has been reported, their role in Th17-driven autoimmunity is still poorly understood. In this study, we identified microRNA (miR)-15b as an important factor in Th17-associated effects and determined that the expression of miR-15b is significantly downregulated in MS patients and in mice with experimental autoimmune encephalomyelitis...
February 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28225507/high-hemoglobin-a1c-and-diabetes-predict-memory-decline-in-the-health-and-retirement-study
#12
Jessica R Marden, Elizabeth R Mayeda, Eric J Tchetgen Tchetgen, Ichiro Kawachi, M Maria Glymour
BACKGROUND: Type 2 diabetes (T2D) is an established risk factor for dementia, but evidence for T2D and memory decline is less consistent. Understanding how T2D and blood glucose relate to memory decline is crucial to elucidating the mechanisms linking T2D and dementia. MATERIALS AND METHODS: For 8888 Health and Retirement Study participants aged 50+, glycosylated hemoglobin (HbA1c) was measured in either 2006 or 2008 and physician's diagnosis of diabetes was self-reported in the same year...
January 2017: Alzheimer Disease and Associated Disorders
https://www.readbyqxmd.com/read/28223691/cellular-glycosylation-affects-herceptin-binding-and-sensitivity-of-breast-cancer-cells-to-doxorubicin-and-growth-factors
#13
Diluka Peiris, Alexander F Spector, Hannah Lomax-Browne, Tayebeh Azimi, Bala Ramesh, Marilena Loizidou, Hazel Welch, Miriam V Dwek
Alterations in protein glycosylation are a key feature of oncogenesis and have been shown to affect cancer cell behaviour perturbing cell adhesion, favouring cell migration and metastasis. This study investigated the effect of N-linked glycosylation on the binding of Herceptin to HER2 protein in breast cancer and on the sensitivity of cancer cells to the chemotherapeutic agent doxorubicin (DXR) and growth factors (EGF and IGF-1). The interaction between Herceptin and recombinant HER2 protein and cancer cell surfaces (on-rate/off-rate) was assessed using a quartz crystal microbalance biosensor revealing an increase in the accessibility of HER2 to Herceptin following deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28223431/changes-in-the-glycosylation-of-kininogen-and-the-development-of-a-kininogen-based-algorithm-for-the-early-detection-of-hcc
#14
Mengjun Wang, Miloslav Sanda, Mary Ann Comunale, Harmin Herrera, Charles Swindell, Yuko Kono, Amit G Singal, Jorge A Marrero, Timothy Block, Radoslav Goldman, Anand Mehta
BACKGROUND: Hepatocellular carcinoma (HCC) has the greatest increase in mortality among all solids tumors in the United States related to low rates of early tumor detection. Development of non-invasive biomarkers for early detection of HCC may reduce HCC-related mortality. METHODS: We have developed an algorithm that combines routinely observed clinical values into a single equation that in a study of > 3,000 patients from 5 independent sites, improved detection of HCC as compared to the currently used biomarker, alpha-feto-protein (AFP), by 4-20%...
February 21, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28223334/corneal-surface-glycosylation-is-modulated-by-il-1r-and-pseudomonas-aeruginosa-challenge-but-is-insufficient-for-inhibiting-bacterial-binding
#15
Amber L Jolly, Paresh Agarwal, Matteo M E Metruccio, David R Spiciarich, David J Evans, Carolyn R Bertozzi, Suzanne M J Fleiszig
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to P. aeruginosa adhesion if it lacks the innate defense protein MyD88 (myeloid differentiation primary response gene 88), or after superficial injury by blotting with tissue paper. Here we explored their effect on corneal surface glycosylation using a metabolic label, tetra-acetylated N-azidoacetylgalactosamine (Ac4GalNAz)...
February 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28223295/a-model-of-chronic-diabetic-polyneuropathy-benefits-from-intranasal-insulin-are-modified-by-sex-and-rage-deletion
#16
Cristiane L de la Hoz, Chu Cheng, Paul Fernyhough, Douglas W Zochodne
Human diabetic polyneuropathy (DPN) is a progressive complication of chronic diabetes mellitus. Preliminary evidence has suggested that intranasal insulin, in doses insufficient to alter hyperglycemia, suppresses the development of DPN. In this work we confirm this finding, but demonstrate that its impact is modified by sex and deletion of RAGE, the receptor for advanced glycosylation endproducts. We serially evaluated experimental DPN in male and female wild type mice and male RAGE null (RN) mice, each with nondiabetic controls, during 16 weeks of diabetes, the final 8 weeks including groups given intranasal insulin...
February 21, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28223252/the-origin-and-evolution-of-basigin-bsg-gene-a-comparative-genomic-and-phylogenetic-analysis
#17
Xinyan Zhu, Shenglan Wang, Mingjie Shao, Jie Yan, Fei Liu
Basigin (BSG), also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147), plays various fundamental roles in the intercellular recognition involved in immunologic phenomena, differentiation, and development. In this study, we aimed to compare the similarities and differences of BSG among organisms and explore possible evolutionary relationships based on the comparison result. We used the extensive BLAST tool to search the metazoan genomes, N-glycosylation sites, the transmembrane region and other functional sites...
February 18, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28222147/the-desert-gerbil-psammomys-obesus-as-a-model-for-metformin-sensitive-nutritional-type-2-diabetes-to-protect-hepatocellular-metabolic-damage-impact-of-mitochondrial-redox-state
#18
Inès Gouaref, Dominique Detaille, Nicolas Wiernsperger, Naim Akhtar Khan, Xavier Leverve, Elhadj-Ahmed Koceir
INTRODUCTION: While metformin (MET) is the most widely prescribed antidiabetic drug worldwide, its beneficial effects in Psammomys obesus (P. obesus), a rodent model that mimics most of the metabolic features of human diabetes, have not been explored thoroughly. Here, we sought to investigate whether MET might improve insulin sensitivity, glucose homeostasis, lipid profile as well as cellular redox and energy balance in P. obesus maintained on a high energy diet (HED). MATERIALS AND METHODS: P...
2017: PloS One
https://www.readbyqxmd.com/read/28222099/morphological-study-of-tooth-development-in-podoplanin-deficient-mice
#19
Kenyo Takara, Naoki Maruo, Kyoko Oka, Chiaki Kaji, Yuji Hatakeyama, Naruhiko Sawa, Yukinari Kato, Junro Yamashita, Hiroshi Kojima, Yoshihiko Sawa
Podoplanin is a mucin-type highly O-glycosylated glycoprotein identified in several somatyic cells: podocytes, alveolar epithelial cells, lymphatic endothelial cells, lymph node stromal fibroblastic reticular cells, osteocytes, odontoblasts, mesothelial cells, glia cells, and others. It has been reported that podoplanin-RhoA interaction induces cytoskeleton relaxation and cell process stretching in fibroblastic cells and osteocytes, and that podoplanin plays a critical role in type I alveolar cell differentiation...
2017: PloS One
https://www.readbyqxmd.com/read/28222023/factors-associated-with-increased-irisin-levels-in-the-type-1-diabetes-mellitus
#20
I Ates, M F Arikan, K Erdogan, M Kaplan, M Yuksel, C Topcuoglu, N Yilmaz, S Guler
OBJECTIVE: The aim of the present study was to determine the irisin levels in patients with the type 1 diabetes mellitus (T1DM) and to examine the relation of irisin levels with the inflammation and autoimmunity. METHODS: This study included 35 cases diagnosed with T1DM and 36 healthy volunteers. Antiglutamic acid decarboxylase (anti-GAD), islet cell antibody (ICA), and insulin autoantibody levels were measured in patients at the time when they were included into the study and recorded from the patient files...
January 1, 2017: Endocrine Regulations
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