keyword
MENU ▼
Read by QxMD icon Read
search

Leukocyte

keyword
https://www.readbyqxmd.com/read/29908901/neonatal-rbc-transfusions-do-benefits-outweigh-risks
#1
REVIEW
Iman F Iskander, Khaled M Salama, Rasha M Gamaleldin, Jerard Seghatchian
Preterm neonates represent one of the most transfused categories of patients. Their target hematocrits, however, are mainly based on expert opinion. The risk of transfusions are very high in the smallest preterm baby with a weak immune response, immature antioxidant ability, fragile germinal matrix and impaired cerebral autoregulation, yet red cell transfusions remain the only life saving measure in the baby with symptomatic anemia. Minimizing phlebotomy losses, following a restrictive transfusion policy and using screened, leukocyte depleted, irradiated, single donor blood remain the best means of avoiding the possible risks while maximizing the benefits of red cell transfusions in the preterm newborn...
May 9, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29908844/tissue-associated-self-antigens-containing-exosomes-role-in-allograft-rejection
#2
Monal Sharma, Ranjithkumar Ravichandran, Sandhya Bansal, Ross M Bremner, Michael A Smith, T Mohanakumar
Exosomes are extracellular vesicles that express self-antigens (SAgs) and donor human leukocyte antigens. Tissue-specific exosomes can be detected in the circulation following lung, heart, kidney and islet cell transplantations. We collected serum samples from patients who had undergone lung (n=30), heart (n=8), or kidney (n=15) transplantations to isolate circulating exosomes. Exosome purity was analyzed by Western blot, using CD9 exosome-specific markers. Tissue-associated lung SAgs, collagen V (Col-V) and K-alpha 1 tubulin (Kα1T), heart SAgs, myosin and vimentin, and kidney SAgs, fibronectin and collagen IV (Col-IV), were identified using western blot...
June 14, 2018: Human Immunology
https://www.readbyqxmd.com/read/29908814/maternal-supply-of-methionine-during-late-pregnancy-is-associated-with-changes-in-immune-function-and-abundance-of-microrna-and-mrna-in-holstein-calf-polymorphonuclear-leukocytes
#3
C B Jacometo, A S Alharthi, Z Zhou, D Luchini, J J Loor
Pregnancy and early life are critical periods during which environmental factors such as nutrition can affect development. Rumen-protected methionine (Met; RPM) supplementation during the prepartum period improves not only performance but immune responses in dairy cows. We investigated the effects of enhanced maternal supply of Met via feeding RPM on whole-blood in vitro lipopolysaccharide (LPS; 0, 0.01, or 5 μg/mL of blood) challenge and targeted microRNA and mRNA abundance in calf blood polymorphonuclear leukocytes (PMNL)...
June 13, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29908485/myeloid-kdm6b-deficiency-results-in-advanced-atherosclerosis
#4
Annette E Neele, Marion J J Gijbels, Saskia van der Velden, Marten A Hoeksema, Marieke C S Boshuizen, Koen H M Prange, Hung-Jen Chen, Jan Van den Bossche, Cindy P P A van Roomen, Annelie Shami, Johannes H M Levels, Jeffrey Kroon, Tina Lucas, Stefanie Dimmeler, Esther Lutgens, Menno P J de Winther
BACKGROUND AND AIMS: Atherosclerosis is a lipid-driven chronic inflammatory disorder of the arteries, and monocytes and macrophages play a central role in this process. Within the atherosclerotic lesion, macrophages can scavenge modified lipids and become the so-called foam cells. We previously reported that the epigenetic enzyme Kdm6b (also known as Jmjd3) controls the pro-fibrotic transcriptional profile of peritoneal foam cells. Given the importance of these cells in atherosclerosis, we now studied the effect of myeloid Kdm6b on disease progression...
June 1, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29908065/a-complex-interplay-between-the-extracellular-matrix-and-the-innate-immune-response-to-microbial-pathogens
#5
REVIEW
Hannah Tomlin, Anna Maria Piccinini
The role of the host extracellular matrix (ECM) in infection tends to be neglected. However, the complex interactions between invading pathogens, host tissues and immune cells occur in the context of the ECM. On the pathogen side, a variety of surface and secreted molecules, including microbial surface components recognizing adhesive matrix molecules (MSCRAMM) and tissue degrading enzymes, are employed that interact with different ECM proteins in order to effectively establish an infection at specific sites...
June 16, 2018: Immunology
https://www.readbyqxmd.com/read/29907934/diagnostic-yield-of-18-f-fdg-pet-ct-in-suspected-diagnosis-of-vascular-graft-infection-a-prospective-cohort-study
#6
Hans Bowles, Juan Ambrosioni, Gaspar Mestres, Marta Hernández-Meneses, Nuria Sánchez, Jaime Llopis, Xavier Yugueros, Manel Almela, Asunción Moreno, Vicenç Riambau, David Fuster, Jose M Miró
BACKGROUND: Prosthetic vascular graft infection (PVGI) is a severe complication associated with high morbidity and mortality. Clinical diagnosis is complex, requiring image testing such as CT angiography or leukocyte scintigraphy, which has considerable limitations. The aim of this study was to know the diagnostic yield of PET/CT with 18 F-Fluorodeoxyglucose (18 F-FDG) in patients with suspected PVGI. METHODS: We performed a prospective cohort study including 49 patients with suspected PVGI, median age of 62 ± 14 years...
June 15, 2018: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/29907903/the-chemokine-ccl4-mip-1%C3%AE-evokes-antinociceptive-effects-in-mice-a-role-for-cd4-lymphocytes-and-met-enkephalin
#7
Mario García-Domínguez, Ana Lastra, Alicia R Folgueras, Rafael Cernuda-Cernuda, María Teresa Fernández-García, Agustín Hidalgo, Luis Menéndez, Ana Baamonde
In the present study, we characterize the antinociceptive effects produced by the chemokine CCL4 in mice. The intraplantar administration of very low doses of CCL4 (0.1-3 pg) produced bilateral antinociception assessed by the unilateral hot-plate test (UHP) without evoking chemotactic responses at the injection site. Moreover, the subcutaneous administration of CCL4 (3-100 pg/kg) also yielded bilateral antinociception in the UHP and the paw pressure test and reduced the number of spinal neurons that express Fos protein in response to noxious stimulation...
June 15, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29907807/availability-of-hla-allele-matched-unrelated-donors-estimation-from-haplotype-frequency-in-the-japanese-population
#8
Satoshi Nishiwaki, Hidenori Tanaka, Hiroto Kojima, Shinichiro Okamoto
A human leukocyte antigen (HLA)-matched unrelated donor is the primary alternative donor for allogeneic hematopoietic cell transplantation in Japan. In considering an optimal donor registry size, the availability of HLA-matched donors is important. In this study, the probability of finding an HLA-A, -B, -C, and -DRB1 allele-matched donor was estimated using two different methods based on the haplotype frequencies in the Japanese population: an actual measurement method (AMM) and a formula method (FM). According to AMM, the probabilities of finding an HLA-matched donor were 40...
June 15, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29907544/impact-of-flt3-receptor-cd135-detection-by-flow-cytometry-on-clinical-outcome-of-adult-acute-myeloid-leukemia-patients
#9
Eman Z Kandeel, Ghada El Sayed, Nahla Elsharkawy, Dalia Negm Eldin, Hanan R Nassar, Dalia Ibrahiem, Randa Amin, Marwa Hanafi, Mohamed Khalil, Azza Kamel
BACKGROUND: The significance of FMS-like tyrosine kinase 3 (FLT3)-ITD mutation in acute myeloid leukemia (AML) prognosis has been well established. The aims of this study were to investigate the prognostic impact of the FLT3 protein (CD135) expression and its association with FLT3-ITD mutation, and to identify its role in minimal residual disease. PATIENTS AND METHODS: CD135 was measured by flow cytometry on leukemic blasts of 257 adults with de novo AML. High expression of CD135 ≥ 20% was correlated with clinical, laboratory, and other prognostic factors that influenced treatment outcome...
May 26, 2018: Clinical Lymphoma, Myeloma & Leukemia
https://www.readbyqxmd.com/read/29907471/optimization-and-in-vivo-evaluation-of-pyrazolopyridines-as-a-potent-and-selective-pi3k%C3%AE-inhibitor
#10
Toshihiro Hamajima, Fumie Takahashi, Koji Kato, Yukihito Sugano, Susumu Yamaki, Ayako Moritomo, Satoshi Kubo, Koji Nakamura, Kaoru Yamagami, Nozomu Hamakawa, Koji Yokoo, Hidehiko Fukahori
Chemical optimization of pyrazolopyridine 1, focused on cellular potency, isoform selectivity and microsomal stability, led to the discovery of the potent, selective and orally available PI3Kδ inhibitor 5d. On the basis of its desirable potency, selectivity and pharmacokinetic profiles, 5d was tested in the trinitrophenylated aminoethylcarboxymethyl-Ficoll (TNP-Ficoll)-induced antibody production model, and showed higher antibody inhibition than a 4-fold oral dose of the starting compound 1. These excellent results suggest that 5d is a potential candidate for further studies in the treatment of autoimmune diseases and leukocyte malignancies...
June 12, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29907459/nlrc5-deficiency-protects-against-acute-kidney-injury-in-mice-by-mediating-carcinoembryonic-antigen-related-cell-adhesion-molecule-1-signaling
#11
Quanxin Li, Ziying Wang, Yan Zhang, Jiaqing Zhu, Liang Li, Xiaojie Wang, Xiaoyang Cui, Yu Sun, Wei Tang, Chengjiang Gao, Chunhong Ma, Fan Yi
There is significant progress in understanding the structure and function of NLRC5, a member of the nucleotide oligomerization domain-like receptor family. However, in the context of MHC class I gene expression, the functions of NLRC5 in innate and adaptive immune responses beyond the regulation of MHC class I genes remain controversial and unresolved. In particular, the role of NLRC5 in the kidney is unknown. NLRC5 was significantly upregulated in the kidney from mice with renal ischemia/reperfusion injury...
June 12, 2018: Kidney International
https://www.readbyqxmd.com/read/29907291/bifunctional-aptamer-mediated-catalytic-hairpin-assembly-for-the-sensitive-and-homogenous-detection-of-rare-cancer-cells
#12
Jumei Liu, Ye Zhang, Qianwen Zhao, Bo Situ, Jiamin Zhao, Shihua Luo, Bo Li, Xiaohui Yan, Pankaj Vadgama, Lei Su, Wen Ma, Wen Wang, Lei Zheng
The presence of cancer cells in body fluids confirms the occurrence of metastasis and guides treatment. A simple, fast, and homogeneous fluorescent method was developed to detect cancer cells based on catalytic hairpin assembly (CHA) and bifunctional aptamers. The bifunctional aptamer had a recognition domain for binding to target cancer cells and an initiator domain for triggering the CHA reaction. In the presence of target cells, the bifunctional aptamer was released from the inhibitor and initiated a cascade reaction of assembly and disassembly of the hairpins...
October 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29907197/the-importance-of-presepsin-value-in-detection-of-gastrointestinal-anastomotic-leak-a-pilot-study
#13
Murat Cikot, Pinar Kasapoglu, Nilgun Isiksacan, Sinan Binboga, Osman Kones, Eyup Gemici, Bahadır Kartal, Halil Alis
BACKGROUND: Early diagnosis of anastomotic leakage is the most important factor in reducing its morbidity and mortality. Anastomotic integrity monitoring of the leukocyte count (WBC), C-reactive protein (CRP), and neutrophil-lymphocyte ratio (NLR) are commonly used laboratory parameters. The availability of follow-up presepsin anastomotic integrity was investigated in this study. MATERIALS AND METHODS: This study included patients who had gastrointestinal anastomosis due to major abdominal surgery between January 2016 and February 2017...
August 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29906569/salival-%C3%AE-iduronidase-activity-as-a-potential-new-biomarker-for-the-diagnosis-and-monitoring-the-effect-of-therapy-in-mucopolysaccharidosis-i
#14
Jaap van Doorn, Brigitte T A van den Broek, Ans J Geboers, Gé-Ann Kuiper, J J Boelens, Peter M van Hasselt
Although disease progression in Mucopolysaccharidosis I (MPS-1) can be attenuated by hematopoietic cell transplantation (HCT), it is increasingly recognized that residual disease is substantial. Biomarkers that would allow us to evaluate the efficacy of HCT (and upcoming new therapies) in non-hematological tissues are needed. Current biomarkers, including the iduronidase (IDUA) activity in leukocytes, are not suitable for this purpose as they are assessed in tissues of hematological origin and may not reflect enzyme availability in non-hematologic tissues...
June 12, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29906454/standardization-of-sampling-and-sample-preparation-for-analysis-of-human-monocyte-subsets-in-peripheral-blood
#15
Ida Marie Rundgren, Øystein Bruserud, Anita Ryningen, Elisabeth Ersvær
INTRODUCTION: Monocytes are important for innate immunity and include the classical (CD14bright CD16negative ), intermediate (CD14bright CD16dim ) and non-classical (CD14dim CD16bright ) monocyte subsets. The quantification of these functionally different subsets in peripheral blood may become useful for diagnosis and follow-up in human diseases. The aim of the present study was to investigate how different pre-analytical parameters influence analysis of monocyte subsets in peripheral blood samples...
June 12, 2018: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29906262/exposure-to-polycyclic-aromatic-hydrocarbons-and-accelerated-dna-methylation-aging
#16
Jun Li, Xiaoyan Zhu, Kuai Yu, Haijing Jiang, Yizhi Zhang, Biqi Wang, Xuezhen Liu, Siyun Deng, Jie Hu, Qifei Deng, Huizhen Sun, Huan Guo, Xiaomin Zhang, Weihong Chen, Jing Yuan, Meian He, Yansen Bai, Xu Han, Bing Liu, Chuanyao Liu, Yanjun Guo, Bing Zhang, Zhihong Zhang, Frank B Hu, Wenjing Gao, Liming Li, Mark Lathrop, Catherine Laprise, Liming Liang, Tangchun Wu
BACKGROUND: Aging is related to an increased risk of morbidity and mortality and is affected by environmental factors. Exposure to polycyclic aromatic hydrocarbons (PAHs) is associated with adverse health outcomes; but the association of such exposure with DNA methylation aging, a novel aging marker, is unclear. OBJECTIVES: Our aim was to investigate the association of PAH exposure with methylation aging. METHODS: We trained and validated a methylation age predictor suitable for Chinese populations using whole blood methylation data in 989 Chinese and 160 Caucasians...
June 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29905830/an-intergenic-variant-rs9268877-between-hla-dra-and-hla-drb-contributes-to-the-clinical-course-and-long-term-outcome-of-ulcerative-colitis
#17
Ho-Su Lee, Suk-Kyun Yang, Myunghee Hong, Seulgi Jung, Byoung Mok Kim, Jung Won Moon, Sang Hyoung Park, Byong Duk Ye, Seak Hee Oh, Kyung Mo Kim, Yong Sik Yoon, Chang Sik Yu, Jiwon Baek, Cue Hyunkyu Lee, Buhm Han, Jianjun Liu, Talin Haritunians, Dermot P B McGovern, Kyuyoung Song
Background and Aims: The genetic contribution to the prognosis of ulcerative colitis (UC) is poorly understood, and most currently known susceptibility loci are not associated with prognosis. To identify genetic variants influencing the prognosis of UC, we performed an Immunochip-based study using an extreme phenotype approach. Methods: Based on the finding that the only association Pdiscovery-meta < 1 × 10-4 was located in the human leukocyte antigen (HLA), we focused our analyses on the HLA region...
June 13, 2018: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/29905014/a-crystal-structure-of-coil-1b-of-vimentin-in-the-filamentous-form-provides-a-model-of-a-high-order-assembly-of-a-vimentin-filament
#18
Allan H Pang, Josiah M Obiero, Arkadiusz W Kulczyk, Vitaliy M Sviripa, Oleg V Tsodikov
Vimentin is an intermediate filament (IF) protein that is expressed in leukocytes, fibroblasts and endothelial cells of blood vessels. Vimentin filaments contribute to structural stability of the cell membrane, organelle positioning and protein transport. Vimentin self-assembles into a dimer that subsequently forms high-order structures, including tetramers and octamers. The details of IF assembly at crystallographic resolutions are limited to the tetrameric form. We describe a crystal structure of a fragment of a vimentin rod domain (coil 1B) with a dimer of tetramers in the asymmetric unit...
June 15, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29904407/the-cardioprotective-effects-of-secretory-leukocyte-protease-inhibitor-against-myocardial-ischemia-reperfusion-injury
#19
Eakkapote Prompunt, Jantira Sanit, Stephanie Barrère-Lemaire, Joel Nargeot, Hannah Noordali, Melanie Madhani, Sarawut Kumphune
Protease enzymes generated from injured cells and leukocytes are the primary cause of myocardial cell damage following ischemia/reperfusion (I/R). The inhibition of protease enzyme activity via the administration of particular drugs may reduce injury and potentially save patients' lives. The aim of the current study was to investigate the cardioprotective effects of treatment with recombinant human secretory leukocyte protease inhibitor (rhSLPI) on in vitro and ex vivo models of myocardial I/R injury. rhSLPI was applied to isolated adult rat ventricular myocytes (ARVMs) subjected to simulated I/R and to ex vivo murine hearts prior to I/R injury...
June 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29904394/relationship-between-25-hydroxyvitamin-d-levels-and-inflammatory-factors-in-children-with-asthma-attack
#20
Shang Wang, Yao Pan, Zhenkun Zhang
The level of 25-hydroxyvitamin D [25-(OH)D] associated with inflammatory factors in children during an asthma attack was investigated. In total, 60 child patients, who were admitted and treated in the Affiliated Children's Hospital of Xuzhou Medical University from March 2015 to March 2017, during their asthma attacks, were selected as the observation group. The patients were divided into the high 25-(OH)D (n=28) and low 25-(OH)D (n=32) groups according to the median level of 25-(OH)D. A total of 30 healthy children were selected as the control group...
June 2018: Experimental and Therapeutic Medicine
keyword
keyword
12690
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"