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https://www.readbyqxmd.com/read/28534074/facial-nerve-sparing-surgery-for-large-vestibular-schwannomas
#1
Paolo Ferroli, Lorenzo Bosio, Morgan Broggi
BACKGROUND: Nowadays, there is a general trend in vestibular schwannoma (VS) surgery favoring near-total or subtotal tumor resection (NTR/STR) with facial nerve (FN) function preservation rather than gross total resection (GTR) with high risk of FN damage. METHODS: The surgical technique of FN sparing in large VS includes patient-tailored image-guided craniotomy, continuous intraoperative neurophysiological monitoring (INM), intracapsular wide tumor debulking, and only final extracapsular dissection with FN course identification and brainstem decompression...
May 22, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28527009/the-simpson-grading-in-meningioma-surgery-does-the-tumor-location-influence-the-prognostic-value
#2
Kira Marie Voß, Dorothee Cäcilia Spille, Cristina Sauerland, Eric Suero Molina, Caroline Brokinkel, Werner Paulus, Walter Stummer, Markus Holling, Astrid Jeibmann, Benjamin Brokinkel
In meningiomas, location-specific differences of the prognostic value of the Simpson classification are sparsely investigated but can influence strategy of surgery. We therefore compared the prognostic value of the Simpson classification in different tumor locations. Progression was compared with Simpson grade in 826 meningioma patients (median age 58 years, female:male ratio 2.4) in location-specific uni- and multivariate analyses. Simpson grade strongly correlated with tumor location (p < .001). Within a median follow-up of 50 months, recurrence was observed in 107 of 803 patients (13%)...
May 19, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28524795/long-term-risk-of-recurrence-and-regrowth-after-gross-total-and-subtotal-resection-of-sporadic-vestibular-schwannoma
#3
Hirofumi Nakatomi, Jeffrey T Jacob, Matthew L Carlson, Shota Tanaka, Minoru Tanaka, Nobuhito Saito, Christine M Lohse, Colin L W Driscoll, Michael J Link
OBJECTIVE The management of vestibular schwannoma (VS) remains controversial. One commonly cited advantage of microsurgery over other treatment modalities is that tumor removal provides the greatest chance of long-term cure. However, there are very few publications with long-term follow-up to support this assertion. The purpose of the current study is to report the very long-term risk of recurrence among a large historical cohort of patients who underwent microsurgical resection. METHODS The authors retrospectively reviewed the medical records of patients who had undergone primary microsurgical resection of unilateral VS via a retrosigmoid approach performed by a single neurosurgeon-neurotologist team between January 1980 and December 1999...
May 19, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28523757/evaluation-of-a-freezer-mill-for-bone-pulverization-prior-to-dna-extraction-an-improved-workflow-for-str-analysis
#4
Emely Morales Colón, Mireya Hernández, Mariel Candelario, María Meléndez, Tracey Dawson Cruz
Traditional methods for bone pulverization typically generate heat, risking stability of DNA sample. SPEX™ has developed cryogenic grinders which introduce liquid nitrogen to cool the sample and aid in the grinding process. In this study, the Freezer Mill 6970 EFM was used with two DNA extraction methods and routine downstream STR analysis procedures. DNA from as little as 0.1 g of bone powder was used to develop full STR profiles after freezer mill pulverization, and the method was reproducible. Further, no contamination was detected upon cleaning/reuse of the sample vials...
May 19, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28523456/bone-marrow-mesenchymal-stem-cell-administration-significantly-improves-outcome-after-retinal-ischemia-in-rats
#5
Biji Mathew, Jacqueline N Poston, John C Dreixler, Leianne Torres, Jasmine Lopez, Ruth Zelkha, Irina Balyasnikova, Maciej S Lesniak, Steven Roth
PURPOSE: Ischemia-associated retinal degeneration is one of the leading causes of vision loss, and to date, there are no effective treatment options. We hypothesized that delayed injection of bone-marrow stem cells (BMSCs) 24 h after the onset of ischemia could effectively rescue ischemic retina from its consequences, including apoptosis, inflammation, and increased vascular permeability, thereby preventing retinal cell loss. METHODS: Retinal ischemia was induced in adult Wistar rats by increasing intraocular pressure (IOP) to 130-135 mmHg for 55 min...
May 18, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28523410/genomic-portrait-of-population-of-jharkhand-india-drawn-with-15-autosomal-strs-and-17-y-strs
#6
Jahangir Imam, Romana Reyaz, Rama Shankar Singh, Arun Kumar Bapuly, Pankaj Shrivastava
We analysed 15 autosomal STRs in 200 unrelated individuals (102 males and 98 females) and 17 Y-STRs in 102 unrelated males living in Jharkhand, India, to establish parameters of forensic interest. The examined autosomal STRs revealed high combined power of exclusion (CPE) and combined power of discrimination (CPD) as equal to 0.9999 and greater than 0.99999, respectively. The combined probability of match (CPm) and combined paternity index (CPI) for all 15 autosomal STR loci were found to be 5.15 × 10(-18) and 6...
May 18, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28513665/a-fully-integrated-microchip-system-for-automated-forensic-short-tandem-repeat-analysis
#7
Junping Han, Wupeng Gan, Bin Zhuang, Jing Sun, Lei Zhao, Jian Ye, Yao Liu, Cai-Xia Li, Peng Liu
We have successfully developed an integrated microsystem that combines two plastic microchips for DNA extraction and PCR amplification with a glass capillary array electrophoresis chip together in a compact control and detection instrument for automated forensic short tandem repeat (STR) analysis. DNA extraction followed by an "in situ PCR" was conducted in a single reaction chamber of the microchip based on a filter paper-based extraction methodology. PCR products were then mixed with sizing standards by an injection electrode and injected into the electrophoresis chip for four-color confocal fluorescence detection...
May 17, 2017: Analyst
https://www.readbyqxmd.com/read/28511096/improved-amplification-results-following-episodes-of-failure-to-amplify-at-the-amelogenin-locus-using-powerplex-%C3%A2-esi-16-fast-system
#8
Sigal Berlyne, Carla Oz, Naftaly Einot, Shlomit Avraham, Tanya Ram, Miri D Goldberg, Ron Gafny
In 2012 the Israel Police DNA Casework laboratory adopted the 16 STR PowerPlex(®) ESI kit for routine use. The Promega Company updated this kit and developed the PowerPlex(®) ESI 16 Fast System in which all autosomal primer pairs remained identical to the original set, except at the amelogenin site. The master mix was improved and optimized which allowed for direct, faster and more robust amplification. Prior to implementing the PowerPlex(®) ESI 16 Fast System in our lab, we conducted a preliminary assay where 213 casework samples were amplified using the new kit...
April 29, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28507140/linkage-disequilibrium-matches-forensic-genetic-records-to-disjoint-genomic-marker-sets
#9
Michael D Edge, Bridget F B Algee-Hewitt, Trevor J Pemberton, Jun Z Li, Noah A Rosenberg
Combining genotypes across datasets is central in facilitating advances in genetics. Data aggregation efforts often face the challenge of record matching-the identification of dataset entries that represent the same individual. We show that records can be matched across genotype datasets that have no shared markers based on linkage disequilibrium between loci appearing in different datasets. Using two datasets for the same 872 people-one with 642,563 genome-wide SNPs and the other with 13 short tandem repeats (STRs) used in forensic applications-we find that 90-98% of forensic STR records can be connected to corresponding SNP records and vice versa...
May 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28506497/comparison-of-enzymatic-hydrolysis-in-a-centrifugal-partition-chromatograph-and-stirred-tank-reactor
#10
J Krause, J Merz
Recently the Centrifugal Partition Chromatography (CPC) device is investigated as a reactor for biocatalytic reactions, as it enables biocatalyst immobilization without solid support and continuous operation of biphasic reaction systems. However, a detailed determination of the enzymes behavior in the CPC reactor and comparison to a classical stirred tank reactor (STR) for biphasic enzyme catalysis is not shown yet. In this study, the performance of an enzymatic biphasic hydrolysis reaction using lipase from Candida rugosa is systematically evaluated using a STR...
May 4, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28504203/internal-validation-of-strmix%C3%A2-for-the-interpretation-of-single-source-and-mixed-dna-profiles
#11
Tamyra R Moretti, Rebecca S Just, Susannah C Kehl, Leah E Willis, John S Buckleton, Jo-Anne Bright, Duncan A Taylor, Anthony J Onorato
The interpretation of DNA evidence can entail analysis of challenging STR typing results. Genotypes inferred from low quality or quantity specimens, or mixed DNA samples originating from multiple contributors, can result in weak or inconclusive match probabilities when a binary interpretation method and necessary thresholds (such as a stochastic threshold) are employed. Probabilistic genotyping approaches, such as fully continuous methods that incorporate empirically determined biological parameter models, enable usage of more of the profile information and reduce subjectivity in interpretation...
April 5, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28503372/endosymbiont-interference-and-microbial-diversity-of-the-pacific-coast-tick-dermacentor-occidentalis-in-san-diego-county-california
#12
Nikos Gurfield, Saran Grewal, Lynnie S Cua, Pedro J Torres, Scott T Kelley
The Pacific coast tick, Dermacentor occidentalis Marx, is found throughout California and can harbor agents that cause human diseases such as anaplasmosis, ehrlichiosis, tularemia, Rocky Mountain spotted fever and rickettsiosis 364D. Previous studies have demonstrated that nonpathogenic endosymbiotic bacteria can interfere with Rickettsia co-infections in other tick species. We hypothesized that within D. occidentalis ticks, interference may exist between different nonpathogenic endosymbiotic or nonendosymbiotic bacteria and Spotted Fever group Rickettsia (SFGR)...
2017: PeerJ
https://www.readbyqxmd.com/read/28496149/the-active-site-structure-and-catalytic-mechanism-of-arsenite-oxidase
#13
Thomas P Warelow, M Jake Pushie, Julien J H Cotelesage, Joanne M Santini, Graham N George
Arsenite oxidase is thought to be an ancient enzyme, originating before the divergence of the Archaea and the Bacteria. We have investigated the nature of the molybdenum active site of the arsenite oxidase from the Alphaproteobacterium Rhizobium sp. str. NT-26 using a combination of X-ray absorption spectroscopy and computational chemistry. Our analysis indicates an oxidized Mo(VI) active site with a structure that is far from equilibrium. We propose that this is an entatic state imposed by the protein on the active site through relative orientation of the two molybdopterin cofactors, in a variant of the Rây-Dutt twist of classical coordination chemistry, which we call the pterin twist hypothesis...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28493563/nalmefene-prevents-alcohol-induced-neuroinflammation-and-alcohol-drinking-preference-in-adolescent-female-mice-role-of-tlr4
#14
Jorge Montesinos, Anabel Gil, Consuelo Guerri
BACKGROUND: We previously showed that, by activating innate immune receptors toll-like 4 (TLR4), adolescent intermittent ethanol exposure causes neuroinflammation, myelin damage and behavioral dysfunctions. Recent findings reveal that clinically-used opioid antagonists naltrexone (NT) and naloxone (NX) inhibit opioid-induced TLR4 signaling, and that NT, NX and nalmefene (NF), the 6-methylene derivative of NX, are able to reduce alcohol drinking escalation. METHODS: NF (0...
May 11, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/28490578/investigating-clinical-issues-by-genotyping-of-medically-important-fungi-why-and-how
#15
REVIEW
Alexandre Alanio, Marie Desnos-Ollivier, Dea Garcia-Hermoso, Stéphane Bretagne
Genotyping studies of medically important fungi have addressed elucidation of outbreaks, nosocomial transmissions, infection routes, and genotype-phenotype correlations, of which secondary resistance has been most intensively investigated. Two methods have emerged because of their high discriminatory power and reproducibility: multilocus sequence typing (MLST) and microsatellite length polymorphism (MLP) using short tandem repeat (STR) markers. MLST relies on single-nucleotide polymorphisms within the coding regions of housekeeping genes...
July 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28488953/atypical-meningioma-referral-patterns-treatment-and-adherence-to-guidelines
#16
Majed Alghamdi, Haocheng Li, Ivo Olivotto, Jay Easaw, John Kelly, Robert Nordal, Gerald Lim
OBJECTIVE: To determine the referral rate to radiation oncologist (RO), use of postoperative radiotherapy (PORT) and the impact of a clinical practice guideline (CPG) on patients with atypical meningioma (AM). METHODS: A retrospective review of meningioma patients (n=526) treated between 2003 and 2013 was undertaken. Patients' characteristics, extent of surgical resection (EOR), RO referral, PORT, date and treatment of first recurrence were collected for all patients >18 years with a new diagnosis of AM after surgical resection (n=83)...
May 2017: Canadian Journal of Neurological Sciences. le Journal Canadien des Sciences Neurologiques
https://www.readbyqxmd.com/read/28487173/patrilineal-background-of-the-she-minority-population-from-chaoshan-fenghuang-mountain-an-isolated-mountain-region-in-china
#17
Shuhui Liu, Guangcan Chen, Haihua Huang, Wengting Lin, Guo Dan, Zhao Shukun, Dongping Tian, Min Su
The She ethnic minority population is distributed in southern China. The origin of the She population has been controversial. The purpose of this work was to investigate the genomic diversity of She. The Chaoshan She population living in the Chaoshan Fenghuang mountain is a relatively isolated population. We detected 14 Y chromosome biallelic markers (Y-SNPs) and 6 Y chromosome short tandem repeat (Y-STR) loci in Chaoshan She people. Y-SNP analysis showed the Chaoshan She was closely related to the Chaoshan Hakka, Chaoshanese, Tujia and Gaoshan national minority...
May 6, 2017: Genomics
https://www.readbyqxmd.com/read/28485820/comparative-tests-of-the-species-genetic-diversity-correlation-at-neutral-and-non-neutral-loci-in-four-species-of-stream-insect
#18
Kozo Watanabe, Michael T Monaghan
A fundamental question linking population genetics and community ecology is how adaptive processes (e.g., natural selection) and neutral processes (e.g., drift-migration equilibrium) underpin the species-genetic diversity correlation (SGDC). Here we combine genome scans and outlier loci detection with community analysis to separately test for neutral and non-neutral SGDCs in four species of stream insect. We sampled 60 localities in Japan and examined the relationships among population AFLP band richness (Br), taxon richness of the total community (S) and of the trophic guild (Str ), and 15 habitat parameters that could potentially drive adaptation and influence richness...
May 9, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28484119/relationship-between-glucose-fluctuations-and-st-segment-resolution-in-patients-with-st-elevation-acute-myocardial-infarction
#19
Keiichi Tsuchida, Norihito Nakamura, Satoshi Soda, Ryohei Sakai, Kota Nishida, Jiro Hiroki, Asami Kashiwa, Yuki Fujihara, Shinpei Kimura, Yukio Hosaka, Kazuyoshi Takahashi, Hirotaka Oda
This study was conducted to assess whether any relationships exist between glucose fluctuations and electrocardiographic surrogate markers of reperfusion injury in patients with ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI).We prospectively studied 63 consecutive patients with STEMI undergoing primary PCI. Patients had either diabetes (n = 30), impaired glucose tolerance (n = 26), impaired fasting glucose (n = 1), or normal glucose tolerance (n = 6). STsegment resolution (STR, %) was measured using electrocardiograms recorded 60 minutes after PCI...
May 8, 2017: International Heart Journal
https://www.readbyqxmd.com/read/28471009/enhancement-of-digestibility-of-casein-powder-and-raw-rice-particles-in-an-improved-dynamic-rat-stomach-model-through-an-additional-rolling-mechanism
#20
Peng Wu, Zhenkai Liao, Tingyu Luo, Liding Chen, Xiao Dong Chen
Previously, a dynamic in vitro rat stomach system (DIVRS-I) designed based on the principles of morphological bionics was reported. The digestibilities of casein powder and raw rice particles were found to be lower than those in vivo due to perhaps the less efficient compression performance and lower mixing efficiency. In this study, a 2nd version of the rat stomach system (DIVRS-II) with an additional rolling extrusion type motility on the wall of the soft-elastic silicone rat stomach model is introduced. The DIVRS-II was then tested by comparing the digestive behaviors of the casein powder suspensions and raw rice particles with those previously published data obtained from the in vivo test on living rats, the DIVRS-I, and the stirred tank reactor at its optimum stirring speed...
May 4, 2017: Journal of Food Science
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