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https://www.readbyqxmd.com/read/29221994/allele-and-haplotype-diversity-of-12-x-strs-in-sardinia
#1
C Robino, D Lacerenza, S Aneli, C Di Gaetano, G Matullo, R Robledo, C Calò
The analysis of clusters of tightly linked X-chromosome short tandem repeat (STR) markers can assist the interpretation of complex kinship cases. However, when linkage disequilibrium (LD) is present in the population of origin of tested individuals, haplotype rather than allele frequencies should be used in likelihood calculations. The diversity of twelve X-STRs arranged in four linkage groups (I: DXS10148-DXS10135-DXS8378; II: DXS7132-DXS10079-DXS10074; III: DXS10103-HPRTB-DXS10101; IV: DXS10146-DXS10134-DXS7423) was tested in a Sardinian population sample (n=516) including three open populations from the Northern, Central and Southern part of the island, and three isolates (Benetutti, Desulo, Carloforte)...
December 5, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29220824/extensive-geographical-and-social-structure-in-the-paternal-lineages-of-saudi-arabia-revealed-by-analysis-of-27-y-strs
#2
Yahya M Khubrani, Jon H Wetton, Mark A Jobling
Saudi Arabia's indigenous population is organized into patrilineal descent groups, but to date, little has been done to characterize its population structure, in particular with respect to the male-specific region of the Y chromosome. We have used the 27-STR Yfiler® Plus kit to generate haplotypes in 597 unrelated Saudi males, classified into five geographical regions (North, South, Central, East and West). Overall, Yfiler® Plus provides a good discrimination capacity of 95.3%, but this is greatly reduced (74...
December 2, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29219779/metastases-of-spinal-myxopapillary-ependymoma-unique-characteristics-and-clinical-management
#3
Theresa Kraetzig, Lily McLaughlin, Mark H Bilsky, Ilya Laufer
OBJECTIVE Myxopapillary ependymoma is a benign WHO Grade I tumor most commonly located in the conus-cauda equina region of the spine. Although this tumor is defined by an overall excellent survival, dissemination throughout the whole neuraxis occurs frequently. The current study evaluated the clinical characteristics and significance of myxopapillary ependymoma metastases. METHODS Patients who underwent surgery from 2005 to 2015 for treatment of spinal myxopapillary ependymoma were included in the study. Charts were reviewed for primary tumor symptoms and initial treatment, local recurrence, response to salvage therapy, and presence and behavior of distant metastases...
December 8, 2017: Journal of Neurosurgery. Spine
https://www.readbyqxmd.com/read/29219271/-polymorphisms-of-19-str-loci-in-guizhou-han-population-and-their-forensic-application
#4
Q S Zhao, Z Ren, H L Zhang, J L Dai, J Wang, F Yu, J Huang
OBJECTIVES: To investigate the allelic distribution of 19 autosomal STR loci in Guizhou Han population, and to estimate the forensic application value. METHODS: The 19 autosomal STR loci in 520 unrelated healthy individuals from Guizhou Han population were studied using Goldeneye™ 20A kit. The 310 genetic analyzer was used for capillary electrophoresis, and the GeneMapper®ID v3.1 for genotyping. RESULTS: The heterozygosis, the discrimination power, the probability of exclusion, the polymorphism information content, the cumulative discrimination power and the cumulative probability of exclusion of the 19 STR loci were 0...
August 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29219270/-genetic-polymorphisms-of-17-y-str-loci-in-nantong-han-population
#5
M Yang, S X Xu, H Y Qin, H Zhang, H Yi, H J Han, D T Dong, Y H Zhang, S L Li
OBJECTIVES: To analyze the genetic phenotypes of Nantong Han population and evaluate the application value of 17 Y-STR loci in Nantong population. METHODS: The peripheral blood samples were collected from 343 unrelated Nantong Han males and the genomic DNA were extracted by Chelex-100 method. Genotyping was performed using the AmpFℓSTR Yfiler™ Kit. The results were compared with other 12 Han populations, including Anhui, Jiangsu, Jiangxi, Shandong, Shanghai, Zhejiang (1), Lanzhou, Nanyang, Luzhou, Mudanjiang, Shanxi and Zhejiang (2), and 9 minority populations (Mongol, Xibe, Tibetan in Lhasa, Tibetan in Qinghai, Kazak, Uighur, Manchu, Paiwan in Taiwan and Tujia)...
August 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29219269/-dna-extraction-from-old-bones-by-automate-express%C3%A2-system
#6
B Li, Z Lü
OBJECTIVES: To establish a method for extracting DNA from old bones by AutoMate Express™ system. METHODS: Bones were grinded into powder by freeze-mill. After extraction by AutoMate Express™, DNA were amplified and genotyped by Identifiler®Plus and MinFiler™ kits. RESULTS: DNA were extracted from 10 old bone samples, which kept in different environments with the postmortem interval from 10 to 20 years, in 3 hours by AutoMate Express™ system...
August 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29219265/-formula-derivation-and-validation-of-probability-of-exclusion-in-the-cases-of-standard-triplet-parentage-testing
#7
M Zhou, H Q Zhang, J Wang
OBJECTIVES: To derive and experiment validate the probability of exclusion (PE) formulas in the cases of standard triplet parentage testing. METHODS: The formulas were derived voluntarily based on the PE definition: [Formula: see text]. This formula was compared with the 5 formulas (1)-(5) reported previously, and the PE values of 19 autosomal STR loci in AGCU EX20 system were calculated. Based on 1 000 samples of single-parentage cases and 1 000 unrelated individuals, the real experiment was designed and the real experiment results of PE were calculated...
August 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29219264/-identification-of-y-chromosomal-genetic-types-for-the-soldier-s-remains-from-huaihai-campaign
#8
C Z Wang, S Q Wen, M S Shi, X E Yu, X J Wang, Y L Pan, Y F Zhang, H Li, J Z Tan
OBJECTIVES: To identify the Y-chromosomal genetic types for the soldier's remains from Huaihai Campaign, and to offer a clue for search of their paternal relatives. METHODS: DNA of the remains were extracted by the ancient DNA extraction method. Yfiler kit was used for the multiplex amplification of 17 Y-STR loci. The haplogroups of the samples were speculated. Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree...
August 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29215049/str-profiling-and-copy-number-variation-analysis-on-single-preserved-cells-using-current-whole-genome-amplification-methods
#9
Ann-Sophie Vander Plaetsen, Lieselot Deleye, Senne Cornelis, Laurentijn Tilleman, Filip Van Nieuwerburgh, Dieter Deforce
The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis. In these applications, target cells are extremely rare and fragile in peripheral circulation, which makes the genetic analysis very challenging. To overcome these challenges, cell stabilization and unbiased whole genome amplification are required. This study investigates the performance of four WGA methods on single or a limited number of cells after 24 hour of Streck Cell-Free DNA BCT preservation...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214499/microbial-degradation-of-forensic-samples-of-biological-origin-potential-threat-to-human-dna-typing
#10
REVIEW
Hirak Ranjan Dash, Surajit Das
Forensic biology is a sub-discipline of biological science with an amalgam of other branches of science used in the criminal justice system. Any nucleated cell/tissue harbouring DNA, either live or dead, can be used as forensic exhibits, a source of investigation through DNA typing. These biological materials of human origin are rich source of proteins, carbohydrates, lipids, trace elements as well as water and, thus, provide a virtuous milieu for the growth of microbes. The obstinate microbial growth augments the degradation process and is amplified with the passage of time and improper storage of the biological materials...
December 6, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29213331/noninvasive-prenatal-diagnosis-of-fetal-aneuploidy-by-circulating-fetal-nucleated-red-blood-cells-and-extravillous-trophoblasts-using-silicon-based-nanostructured-microfluidics
#11
Chung-Er Huang, Gwo-Chin Ma, Hei-Jen Jou, Wen-Hsiang Lin, Dong-Jay Lee, Yi-Shing Lin, Norman A Ginsberg, Hsin-Fu Chen, Frank Mau-Chung Chang, Ming Chen
Background: Noninvasive prenatal testing (NIPT) based on cell-free DNA in maternal circulation has been accepted worldwide by the clinical community since 2011 but limitations, such as maternal malignancy and fetoplacental mosaicism, preclude its full replacement of invasive prenatal diagnosis. We present a novel silicon-based nanostructured microfluidics platform named as "Cell Reveal™" to demonstrate the feasibility of capturing circulating fetal nucleated red blood cells (fnRBC) and extravillous cytotrophoblasts (EVT) for cell-based noninvasive prenatal diagnosis (cbNIPD)...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/29211512/durability-of-second-antiretroviral-regimens-in-the-italian-cohort-naive-antiretrovirals-foundation-study-and-factors-associated-with-discontinuation
#12
Antonio Di Biagio, Patrizia Lorenzini, Giulia Gustinetti, Stefano Rusconi, Andrea De Luca, Giuseppe Lapadula, Sergio Lo Caputo, Stefania Cicalini, Francesco Castelli, Giulia Marchetti, Andrea Antinori, Antonella d'Arminio Monforte
The study was designed to investigate the median duration of second antiretroviral regimens and factors associated with early discontinuation in HIV patients who switched with an undetectable viral load. We conducted a retrospective analysis of the Italian Cohort Naive Antiretrovirals Foundation Study (ICONA), which collects data throughout the country. Patients who started first antiretroviral therapy (ART) after January 1, 2008 in any center involved in this cohort and then switched to a second regimen were included in the study...
December 2017: AIDS Patient Care and STDs
https://www.readbyqxmd.com/read/29209189/spike-triggered-regression-for-synaptic-connectivity-reconstruction-in-neuronal-networks
#13
Yaoyu Zhang, Yanyang Xiao, Douglas Zhou, David Cai
How neurons are connected in the brain to perform computation is a key issue in neuroscience. Recently, the development of calcium imaging and multi-electrode array techniques have greatly enhanced our ability to measure the firing activities of neuronal populations at single cell level. Meanwhile, the intracellular recording technique is able to measure subthreshold voltage dynamics of a neuron. Our work addresses the issue of how to combine these measurements to reveal the underlying network structure. We propose the spike-triggered regression (STR) method, which employs both the voltage trace and firing activity of the neuronal population to reconstruct the underlying synaptic connectivity...
2017: Frontiers in Computational Neuroscience
https://www.readbyqxmd.com/read/29203868/a-microsatellite-repeat-in-pca3-long-non-coding-rna-is-associated-with-prostate-cancer-risk-and-aggressiveness
#14
John Lai, Leire Moya, Jiyuan An, Andrea Hoffman, Srilakshmi Srinivasan, Janaththani Panchadsaram, Carina Walpole, Joanna L Perry-Keene, Suzanne Chambers, Melanie L Lehman, Colleen C Nelson, Judith A Clements, Jyotsna Batra
Short tandem repeats (STRs) are repetitive sequences of a polymorphic stretch of two to six nucleotides. We hypothesized that STRs are associated with prostate cancer development and/or progression. We undertook RNA sequencing analysis of prostate tumors and adjacent non-malignant cells to identify polymorphic STRs that are readily expressed in these cells. Most of the expressed STRs in the clinical samples mapped to intronic and intergenic DNA. Our analysis indicated that three of these STRs (TAAA-ACTG2, TTTTG-TRIB1, and TG-PCA3) are polymorphic and differentially expressed in prostate tumors compared to adjacent non-malignant cells...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29201383/an-autosomal-recessive-mutation-in-scl24a4-causing-enamel-hypoplasia-in-samoyed-and-its-relationship-to-breed-wide-genetic-diversity
#15
Niels C Pedersen, Bonnie Shope, Hongwei Liu
Background: Pure breeding of dogs has led to over 700 heritable disorders, of which almost 300 are Mendelian in nature. Seventy percent of the characterized mutations have an autosomal recessive mode of inheritance, indicative of positive selection during bouts of inbreeding primarily for new desired conformational traits. Samoyed suffer from several common complex genetic disorders, but up to this time only two X-linked and one autosomal dominant disorder have been identified. Previous studies based on pedigrees and SNP arrays have concluded that Samoyed breeders have done a good job in maintaining genetic diversity and avoiding excessive inbreeding...
2017: Canine Genetics and Epidemiology
https://www.readbyqxmd.com/read/29200741/enzyme-inhibitors-cause-multiple-effects-on-accumulation-of-monoterpene-indole-alkaloids-in-catharanthus-roseus-cambial-meristematic-cell-cultures
#16
Zhou Pengfei, Zhu Jianhua, Yu Rongmin, Zi Jiachen
Background: Enzyme inhibitors have been used for the clarification of biosynthesis of natural products. Catharanthus roseus cambial meristematic cell (CMC) culture has been established and proved to be a better monoterpeneindole alkaloid (MIA) producer than C. roseus dedifferentiated cell (DDC) culture. However, little is known about the inter-relationship of the MIA-biosynthetic genes with respect to their transcription. Objective: To clarify effects of alteration of one gene transcription on transcript levels of another genes in MIA-biosynthetic pathway, and how the accumulation of MIAs in CMCs are influenced by the alteration of their biosynthetic gene transcript levels...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29199580/what-is-ganoderma-lucidum-in-the-molecular-era
#17
Viktor Papp, Bálint Dima, Solomon P Wasser
The scientific name Ganoderma lucidum has long been used in the global medicinal mushroom market, and many publications have proven its pharmacological properties and chemical components. Although several Ganoderma species have been described worldwide as having morphology considerably similar to that of G. lucidum s. str., this binomial is still the most frequently used in pharmacological studies. However, the use of particularly divergent nomenclature has caused major difficulties in the evaluation of the results of pharmacological studies...
2017: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/29198841/-antimicrobial-susceptibility-in-isolates-of-streptococcus-equi-subsp-equi-from-buenos-aires-province-argentina
#18
Carla P Bustos, María J Marfil, Natalia S Lanza, Nora Guida
Streptococcus equi subsp. equi is the etiologic agent of strangles, an infectious disease affecting the upper respiratory tract and head and neck lymph nodes of equines. Routine antimicrobial therapy includes penicillin (PEN) as antibiotic of first choice. Streptococci are usually susceptible to PEN and only a few antimicrobial studies had been performed. The aim of this work was to study the antimicrobial susceptibility profile of S. equi from Buenos Aires, Argentina. Ninety-two isolates were studied by the single disk method to PEN, cefotaxime, erythromycin (ERY), tetracycline, enrofloxacin (ENR), trimethoprim sulfamethoxazole (TMS), ciprofloxacin, clindamycin (CLI), streptomycin (STR) and florfenicol...
November 30, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29197717/inferring-chinese-surnames-with-y-str-profiles
#19
Cheng-Min Shi, Changzhen Li, Liang Ma, Lianjiang Chi, Jing Zhao, Wuzhou Yuan, Zhendiao Zhou, Jiang-Wei Yan, Hua Chen
Co-ancestry of human surnames and Y-chromosomes in most human populations and social groups suggests the possibility of inferring one from the other. However, such an intuitive perspective remains to be formally explored. In the present study, we develop two computational methods, based on cosine distance (dcos) and coalescence distance (dcoal) respectively, to infer surnames from Y-STR profiles. We also survey Y-STR variations at 15 loci for 19,009 individuals of Shandong Province in China. For a total of 266 surnames included in the data set, our methods can pinpoint to a single surname with an average accuracy of 65%, and with an average accuracy higher than 80% when providing >4 candidate surnames...
November 24, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29194608/str-genotyping-from-a-dry-cleaned-skirt-in-a-sexual-assault-case
#20
Hisae Ogawa, Yuuji Hiroshige, Takashi Yoshimoto, Akira Ishii, Toshimichi Yamamoto
In this sexual assault case, the standard preliminary semen examinations could not confirm physically or biochemically whether the accused's semen had stained the victim's skirt because the skirt had been dry-cleaned for stain removal and had been worn for more than a year after the assault. Fortunately, however, a photograph taken just after the assault was found in the court records that showed white stains on the checkered skirt. The locations of the stains were estimated based on the checkered pattern of the fabric, and microscopic examination using Baecchi's staining revealed the presence of spermatozoa...
December 1, 2017: Journal of Forensic Sciences
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