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https://www.readbyqxmd.com/read/29677577/dna-typing-of-birch-development-of-a-forensic-str-system-for-betula-pendula-and-betula-pubescens
#1
Monique Wesselink, Aleksandar Dragutinović, Jeroen W Noordhoek, Leonie Bergwerff, Irene Kuiper
Although botanical trace evidence is often encountered in case investigations, the utilization of such traces in forensic investigations is still limited. Development of a forensic STR system for the two species of Betula (birch) indigenous to and abundant in North West Europe is a step in enhancing the applicability of traces from these species. We describe six microsatellite markers developed for birch species in detail, including repeat structure, and we propose a nomenclature for the encountered alleles...
April 7, 2018: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29677481/mutation-analysis-of-short-tandem-repeats-in-a-population-sample-from-upper-silesia-southern-poland
#2
Kornelia Droździok, Jadwiga Kabiesz, Marcin Tomsia, Rafał Skowronek, Krzysztof Rębała
In paternity testing, DNA polymorphism analysis not only settles explicitly disputed paternity issue but also provides information on mutation frequencies in STR loci. In this study, insertion or deletion of one repetitive unit was observed in 38 of 32,391 meiotic transfers analysed in 953 paternity testing cases. Parentage samples from Upper Silesia (southern Poland) were examined in 2008-2014 with the use of three commercially available amplification kits: AmpFlSTR Identifiler (Applied Biosystems), PowerPlex 16 HS (Promega) and PowerPlex ESX 17 (Promega)...
April 1, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29675433/sweet-taste-receptors-mediated-ros-nlrp3-inflammasome-signaling-activation-implications-for-diabetic-nephropathy
#3
Luping Zhou, Wei Huang, Youhua Xu, Chenlin Gao, Ting Zhang, Man Guo, Yan Liu, Jingya Ding, Ludan Qin, Zihao Xu, Yang Long, Yong Xu
Previous studies demonstrated that ROS-NLRP3 inflammasome signaling activation was involved in the pathogenesis of diabetic nephropathy (DN). Recent research has shown that sweet taste receptors (STRs) are important sentinels of innate immunity. Whether high glucose primes ROS-NLRP3 inflammasome signaling via STRs is unclear. In this study, diabetic mouse model was induced by streptozotocin (STZ) in vivo; mouse glomerular mesangial cells (GMCs) and human proximal tubular cells were stimulated by high glucose (10, 20, and 30 mmol/L) in vitro; STR inhibitor lactisole was used as an intervention reagent to evaluate the role and mechanism of the STRs in the pathogenesis of DN...
2018: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29674926/a-taxonomic-study-of-quercus-langbianensis-complex-based-on-morphology-and-dna-barcodes-of-classic-and-next-generation-sequences
#4
Hoang Thi Binh, Nguyen Van Ngoc, Shuichiro Tagane, Hironori Toyama, Keiko Mase, Chika Mitsuyuki, Joeri Sergej Strijk, Yoshihisa Suyama, Tetsukazu Yahara
The taxonomy of Quercus langbianensis and its relatives in Vietnam and Cambodia have been revised based on evidence obtained from field observations, morphological comparison of herbarium specimens and molecular analyses using both classic and next generation DNA markers. Based on Bayesian inference using rbc L, mat K and ITS regions and Neighbour-joining tree using genome-wide sequences amplified with multiplexed inter-simple sequence repeat (ISSR) primers (MIG-seq), the authors recognised ten species in the complex in Vietnam and Cambodia, three of which are newly described in this paper: Q...
2018: PhytoKeys
https://www.readbyqxmd.com/read/29666998/male-dna-under-female-fingernails-after-scratching-transfer-and-persistence-evaluation-by-rt-pcr-analysis-and-y-str-typing
#5
Alessandra Iuvaro, Carla Bini, Silvia Dilloo, Stefania Sarno, Susi Pelotti
The collection of biological debris beneath fingernails can be useful in forensic casework when a struggle between the victim and the offender is suspected. In the present study, we set up a controlled scratching experiment in which female volunteers scratched the male volunteers' forearms, simulating a defensive action during an assault. A total of 160 fingernail samples were collected: 80 "control samples" before the scratching, 40 samples immediately after the scratching (t = 0 h), and 40 samples 5 h after the scratching (t = 5 h)...
April 17, 2018: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/29665929/-anti-cd36-mediated-platelet-transfusion-refractoriness-and-related-cases-after-stem-cell-transplantation
#6
Yan Zhou, Li-Lan Li, Zhou-Lin Zhong, Xue-Jun Liu, Jin-Lian Liu, Wei-Dong Shen, Guo-Guang Wu
OBJECTIVE: To analyse the cases of platelet transfusion refractoriness after received HLA-matched unrelated donor hematopoietic stem cell transplantation, to analyze and identify the phenotype and genotype of CD36 in both the patient and stem cell donor, as well as the characteristic of antibody induced platelet transfusion refractoriness, and to analyse the efficacy of matched CD36-deficiency platelets transfusions. METHODS: The CD36 expression on platelet and monocyte was analyzed by flow cytometry (FCM) in both patient and donor...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29663254/novel-insights-into-the-genetic-background-of-genetically-modified-mice
#7
Peter Dobrowolski, Melina Fischer, Ronald Naumann
Unclear or misclassified genetic background of laboratory rodents or a lack of strain awareness causes a number of difficulties in performing or reproducing scientific experiments. Until now, genetic differentiation between strains and substrains of inbred mice has been a challenge. We have developed a screening method for analyzing inbred strains regarding their genetic background. It is based on 240 highly informative short tandem repeat (STR) markers covering the 19 autosomes as well as X and Y chromosomes...
April 16, 2018: Transgenic Research
https://www.readbyqxmd.com/read/29661278/comparing-survival-outcomes-of-gross-total-resection-and-subtotal-resection-with-radiotherapy-for-craniopharyngioma-a-meta-analysis
#8
Guoqing Wang, Xiaoyang Zhang, Mengzhao Feng, Fuyou Guo
BACKGROUND: Recent studies suggest that subtotal resection (STR) followed by radiation therapy (RT) is an appealing alternative to gross total resection (GTR) for craniopharyngioma, but it remains controversial. We conducted a meta-analysis to determine whether GTR is superior to STR with RT for craniopharyngioma. MATERIALS AND METHODS: A systematic search was performed for articles published until October 2017 in the PubMed, Embase, and Cochrane Central databases...
June 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29660126/grigorasnps-optimized-analysis-of-snps-for-dna-forensics
#9
Darrell O Ricke, Anna Shcherbina, Adam Michaleas, Philip Fremont-Smith
High-throughput sequencing (HTS) of single nucleotide polymorphisms (SNPs) enables additional DNA forensic capabilities not attainable using traditional STR panels. However, the inclusion of sets of loci selected for mixture analysis, extended kinship, phenotype, biogeographic ancestry prediction, etc., can result in large panel sizes that are difficult to analyze in a rapid fashion. GrigoraSNP was developed to address the allele-calling bottleneck that was encountered when analyzing SNP panels with more than 5000 loci using HTS...
April 16, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29656213/molecular-characterization-of-voltage-gated-potassium-channel-kv-and-its-importance-in-functional-dynamics-in-bull-spermatozoa
#10
Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100 μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106  cells), vehicle control (99 μL of SDM containing 10 × 106  cells, and DMSO- 1  μL) and 4-AP treatment group (99 μL of SDM containing 10 × 106  cells, and drug 1 μL 4-AP) were used in the study...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29655768/cell-free-dna-and-microvascular-damage-in-st-segment-elevation-myocardial-infarction-treated-with-primary-percutaneous-coronary-intervention
#11
Juan Sanchis, Sergio García-Blas, Luis Ortega-Paz, Ana Paula Dantas, Enrique Rodríguez, Lidia Abellán, Salvatore Brugaletta, Ernesto Valero, Gema Miñana, Manuel Garabito, África Corchón, Julio Núñez, Arturo Carratalá, Manel Sabaté
INTRODUCTION AND OBJECTIVES: Cell-free DNA (cfDNA) in ST-segment elevation myocardial infarction might originate from hyperactivated leukocytes at the coronary lesion. Our aim was to investigate the relationship between cfDNA and coronary reperfusion. METHODS: We studied 116 patients treated with primary angioplasty using thrombus aspiration. Coronary (during aspiration) and peripheral (at the end of the procedure) blood samples were drawn for cfDNA, as well as high-sensitivity troponin T and myeloperoxidase quantification...
April 11, 2018: Revista Española de Cardiología
https://www.readbyqxmd.com/read/29653142/characterization-of-a-conjunctival-melanoma-cell-line-cm-as16-newly-established-from-a-metastatic-han-chinese-patient
#12
Yongyun Li, Qingfeng Shang, Peng Li, Jinfeng Cao, Liqi Zhu, Martine J Jager, Xianqun Fan, Shengfang Ge, Renbing Jia
Conjunctival melanoma (CM) is associated with metastases formation, can be fatal, and occurs in all different races. While cell lines are essential for experimental research, all available CM cell lines are derived from Caucasian patients. Furthermore, they are not derived from metastases. We aimed to establish a new CM cell line from a parotid metastasis in a Han Chinese patient and to depict its characteristics. The novel cell line, CM-AS16, was obtained from a surgical parotid sample and determined as a unique one with short tandem repeat (STR) analysis...
April 10, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29652998/-application-of-quantitative-fluorescencet-pcr-in-the-prenatal-diagnosis-of-chromosomale-aneuploidies
#13
Shengfang Qin, Xueyan Wang, Ximin Chen, Mengling Ye, Chun Chen, Ping Wei, Lan Zeng, Yi Deng, Yunxing Li, Na Xi, Xiao Song, Lingling Sun
OBJECTIVE: To assess the accuracy of quantitative fluorescence PCR(QF-PCR) for the detection of fetal chromosomal aneuploidies and its values for prenatal diagnosis. METHODS: QF-PCR and chromosomal karyotyping were used to analyze 6066 amniotic fluid samples derived from 6034 pregnant women. RESULTS: Both QF-PCR and karyotyping analysis have detected 135 cases of fetal aneuploidies involving chromosomes 21, 18, 13, X, and Y. The QF-PCR assay was also successful in 67 cases for which amniotic fluid culture has failed...
April 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/29651125/genetic-diversity-and-phylogenetic-characteristics-of-chinese-tibetan-and-yi-minority-ethnic-groups-revealed-by-non-codis-str-markers
#14
Guanglin He, Zheng Wang, Xing Zou, Xu Chen, Jing Liu, Mengge Wang, Yiping Hou
Non-CODIS STRs, with high polymorphism and allele frequency difference among ethnically and geographically different populations, play a crucial role in population genetics, molecular anthropology, and human forensics. In this work, 332 unrelated individuals from Sichuan Province (237 Tibetan individuals and 95 Yi individuals) are firstly genotyped with 21 non-CODIS autosomal STRs, and phylogenetic relationships with 26 previously investigated populations (9,444 individuals) are subsequently explored. In the Sichuan Tibetan and Yi, the combined power of discrimination (CPD) values are 0...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649681/forensic-parameters-for-15-autosomal-strs-in-mestizo-population-from-the-state-of-guerrero-south-mexico
#15
G J Locia-Aguilar, B López-Saucedo, S Deheza-Bautista, O V Salado-Beltrán, V M Martínez-Sevilla, H Rangel-Villalobos
Allele distribution and forensic parameters were estimated for 15 STR loci (AmpFlSTR Identifiler kit) in 251 Mexican-Mestizos from the state of Guerrero (South, Mexico). Genotype distribution was in agreement with Hardy-Weinberg expectations for all 15 STRs. Similarly, linkage disequilibrium test demonstrated no association between pair of loci. The power of exclusion and power of discrimination values were 99.999634444% and >99.99999999%, respectively. Genetic relationship analysis regarding Mestizo populations from the main geographic regions of Mexico suggests that the Center and the present South regions conform one population cluster, separated from the Southeast and Northwest regions...
March 31, 2018: Legal Medicine
https://www.readbyqxmd.com/read/29649649/aberrant-expression-of-erk-and-hpgd-indicates-radiation-resistance-and-poor-prognosis-for-patients-with-clival-chordomas
#16
Yixuan Zhai, Jiwei Bai, Shuai Wang, Mingxuan Li, Fei Wang, Chuzhong Li, Yazhuo Zhang
OBJECTIVE: We aim to explore the molecular markers of radiosensitivity and prognostic factors in clival chordomas. METHODS: A total of 35 patients were reviewed retrospectively. The mean follow-up interval was 66.37 months (range: 29-106 months). The Kaplan-Meier method was used for survival analysis. Immunohistochemical staining was used to detect the expression level of ERK and HPGD. RESULTS: Total resection (TR) was achieved in 12 cases, subtotal resection (STR) in 12 cases, partial resection (PR) in 11 cases...
April 9, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29643326/identification-and-characterization-of-short-tandem-repeats-in-the-tibetan-macaque-genome-based-on-resequencing-data
#17
San-Xu Liu, Wei Hou, Xue-Yan Zhang, Chang-Jun Peng, Bi-Song Yue, Zhen-Xin Fan, Jing Li
The Tibetan macaque, which is endemic to China, is currently listed as a Near Endangered primate species by the International Union for Conservation of Nature (IUCN). Short tandem repeats (STRs) refer to repetitive elements of genome sequence that range in length from 1-6 bp. They are found in many organisms and are widely applied in population genetic studies. To clarify the distribution characteristics of genome-wide STRs and understand their variation among Tibetan macaques, we conducted a genome-wide survey of STRs with next-generation sequencing of five macaque samples...
April 11, 2018: Zoological Research
https://www.readbyqxmd.com/read/29642498/monitoring-of-the-apple-fruit-moth-detection-of-genetic-variation-and-structure-applying-a-novel-multiplex-set-of-19-str-markers
#18
Abdelhameed Elameen, Hans Geir Eiken, Ida Fløystad, Geir Knudsen, Snorre B Hagen
The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan ( Sorbus aucuparia ) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2-4 years, and apple ( Malus domestica ) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella ...
April 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29628767/interaction-between-rpsl-and-gyra-mutations-affects-the-fitness-and-dual-resistance-of-mycobacterium-tuberculosis-clinical-isolates-against-streptomycin-and-fluoroquinolones
#19
Honghu Sun, Jumei Zeng, Song Li, Pengkuan Liang, Chao Zheng, Yong Liu, Tao Luo, Nalin Rastogi, Qun Sun
Background: The interaction between different drug-resistant mutations is important to the development of drug resistance and its evolution. In this study, we aimed to reveal the potential relationships between mutations conferring resistance to two important antituberculosis drugs streptomycin (STR) and fluoroquinolones (FLQ). Materials and methods: We used an in vitro competitive fitness assay to reveal the interactions between different mutations of rpsL and gyrA in drug-resistant Mycobacterium smegmatis , followed by the analysis of the frequency of rpsL and gyrA mutation combinations in 213 STR-FLQ dual-resistant clinical Mycobacterium tuberculosis isolates from Sichuan region, which was also investigated by the whole genome data from 3,056 global clinical M...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29628666/cardiopulmonary-resuscitation-unusual-techniques-for-unusual-situations
#20
Vidhu Bhatnagar, Kavitha Jinjil, Deepak Dwivedi, Rohit Verma, Urvashi Tandon
Background: The cardiopulmonary resuscitation (CPR) in prone position has been dealt with in 2010 American Heart Association (AHA) guidelines but have not been reviewed in 2015 guidelines. The guidelines for patients presenting with cardiac arrest under general anesthesia in lateral decubitus position and regarding resuscitation in confined spaces like airplanes are also not available in AHA guidelines. This article is an attempt to highlight the techniques adopted for resuscitation in these unusual situations...
January 2018: Journal of Emergencies, Trauma, and Shock
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