keyword
MENU ▼
Read by QxMD icon Read
search

Glycocalyx

keyword
https://www.readbyqxmd.com/read/29773228/changes-in-mucins-and-matrix-metalloproteases-in-the-endometrium-of-early-pregnant-alpacas-vicugna-pacos
#1
Daniela E Barraza, Renato Zampini, Silvana A Apichela, Joel I Pacheco, Martin E Argañaraz
South American Camelids (SAC) have unique reproductive features, one of which is that 98% of the pregnancies develop in the left uterine horn. Furthermore, early pregnancy is an uncharacterized process in these species, especially in regard to the ultrastructural, biochemical and genetic changes that the uterine epithelial surface undergoes to allow embryo implantation. The present study describes the uterine horn luminal surface and the characteristics of the mucinous glycocalyx in non-pregnant and early pregnant (15 days) female alpacas...
May 14, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29765073/oncotically-driven-control-over-glycocalyx-dimension-for-cell-surface-engineering-and-protein-binding-in-the-longitudinal-direction
#2
Erika M J Siren, Rafi Chapanian, Iren Constantinescu, Donald E Brooks, Jayachandran N Kizhakkedathu
Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing for reactivity control in the longitudinal direction ('z'-direction) on the glycocalyx. Studies in HUVECs demonstrated an oncotically driven collapse of the glycocalyx brush structure in the presence of crowders as the mechanism responsible for re-directing reactivity...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29750566/advanced-age-results-in-a-diminished-endothelial-glycocalyx
#3
Daniel R Machin, Samuel I Bloom, Robert A Campbell, Tam T T Phuong, Phillip Ellis Gates, Lisa A Lesniewski, Matthew T Rondina, Anthony John Donato
Age-related microvascular dysfunction is well characterized in rodents and humans, but little is known about the properties of the microvascular endothelial glycocalyx in advanced age. We examined the glycocalyx in microvessels of young and old C57BL6 mice (young: 6.1{plus minus}0.1 vs old: 24.6{plus minus}0.2 mo) using intravital microscopy and transmission electron microscopy, and in human participants (young: 27{plus minus}1 vs old: 61{plus minus}2 yr) using intravital microscopy. Glycocalyx thickness in mesenteric and skeletal muscle microvessels was 51-54% lower in old, compared to young mice...
May 11, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29747825/gangliosides-in-membrane-organization
#4
Sandro Sonnino, Elena Chiricozzi, Sara Grassi, Laura Mauri, Simona Prioni, Alessandro Prinetti
Since the structure of GM1 was elucidated 55years ago, researchers have been attracted by the sialylated glycans of gangliosides. Gangliosides head groups, protruding toward the extracellular space, significantly contribute to the cell glycocalyx; and in certain cells, such as neurons, are major determinants of the features of the cell surface. Expression of glycosyltransferases involved in the de novo biosynthesis of gangliosides is tightly regulated along cell differentiation and activation, and is regarded as the main metabolic mechanism responsible for the acquisition of cell-specific ganglioside patterns...
2018: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29746166/endothelial-barrier-function-is-differentially-regulated-by-ceacam1-mediated-signaling
#5
Sharang Ghavampour, Florian Kleefeldt, Heike Bömmel, Julian Volland, Alexander Paus, Andrea Horst, Verena Pfeiffer, Stefan Hübner, Nicole Wagner, Uwe Rueckschloss, Süleyman Ergün
Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) is known to be crucial to vasculogenesis and angiogenesis. Recently, CEACAM1 deficiency was shown to result in the formation of aortic plaque-like lesions, indicating a role for CEACAM1 in adult vessels as well. The underlying mechanisms remained largely elusive. Therefore, we aimed to elucidate the role of CEACAM1 in endothelial homeostasis. Here, we show that CEACAM1 deficiency causes subcellular eNOS redistribution in endothelial cells ( i...
May 10, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29730504/sparc-preserves-endothelial-glycocalyx-integrity-and-protects-against-adverse-cardiac-inflammation-and-injury-during-viral-myocarditis
#6
Marieke Rienks, Paolo Carai, Jurgen van Teeffelen, Bart Eskens, Wouter Verhesen, Bianca Hemmeryckx, Daniel M Johnson, Rick van Leeuwen, Elizabeth A Jones, Stephane Heymans, Anna-Pia Papageorgiou
Myocardial damage as a consequence of cardiotropic viruses leads to a broad variety of clinical presentations and is still a complicated condition to diagnose and treat. Whereas the extracellular matrix protein Secreted Protein Acidic and Rich in Cysteine or SPARC has been implicated in hypertensive and ischemic heart disease by modulating collagen production and cross-linking, its role in cardiac inflammation and endothelial function is yet unknown. Absence of SPARC in mice resulted in increased cardiac inflammation and mortality, and reduced cardiac systolic function upon coxsackievirus-B3 induced myocarditis...
May 3, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29728805/large-volume-crystalloid-fluid-is-associated-with-increased-hyaluronan-shedding-and-inflammation-in-a-canine-hemorrhagic-shock-model
#7
Lisa Smart, C J Boyd, M A Claus, E Bosio, G Hosgood, A Raisis
Shedding of the endothelial glycocalyx precedes leukocyte activation and adherence in acute inflammation. Rapid administration of crystalloid or colloid fluids for treating hemorrhagic shock may cause endothelial glycocalyx shedding, thereby increasing inflammation. This study aimed to compare the effect of different fluid treatments in a canine shock model on glycocalyx biomarker, hyaluronan, and inflammatory biomarkers. Greyhound dogs under general anesthesia subject to hemorrhage for 60 min were given 20 mL kg-1 of either fresh whole blood (FWB), hydroxyethyl starch (HES) 130/0...
May 4, 2018: Inflammation
https://www.readbyqxmd.com/read/29725817/assembling-the-thickest-plant-cell-wall-exine-development-in-echinops-asteraceae-cynareae
#8
Nina I Gabarayeva, Svetlana V Polevova, Valentina V Grigorjeva, Stephen Blackmore
The exceptionally complex exine of Echinops, representing a significant investment of energy, develops from an elaborate glycocalyx which establishes, by self-assembly, a multi-layered system of micelles upon which sporopollenin polymerizes. We report on pollen development in two species of Echinops (Asteraceae, Cynareae) studied using transmission and scanning electron microscopy with an emphasis on the organisation and development of the massive sporoderm (maximum thickness 18 μm). The major events of exine deposition during the tetrad stage follow the now familiar sequence of self-assembling micellar mesophases and the subsequent incorporation of sporopollenin, observed here as: (1) spherical units with light cores; (2) columns of spherical units with dark cores; (3) large branched macromolecules arranged in a dendritic, three-dimensional network of long alveoli; and (4) alveoli with electron-transparent cores and dense walls...
May 3, 2018: Planta
https://www.readbyqxmd.com/read/29724540/glycocalyx-degradation-and-inflammation-in-cardiac-surgery
#9
Eero Pesonen, Arie Passov, Sture Andersson, Raili Suojaranta, Tomi Niemi, Peter Raivio, Markku Salmenperä, Alexey Schramko
OBJECTIVE: Experimental inflammation induces degradation of glycocalyx. The authors hypothesized that inflammation is an important determinant of glycocalyx degradation in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). DESIGN: A prospective observational study. SETTING: Operation theater and intensive care unit of a university hospital. PARTICIPANTS: Two separate prospective patient cohorts. INTERVENTIONS: Blood samples were collected at 5 perioperative time points in the trial cohort (30 patients) and only preoperatively in the preoperative cohort (35 patients)...
April 3, 2018: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/29721496/the-role-of-plasma-transfusion-in-massive-bleeding-protecting-the-endothelial-glycocalyx
#10
REVIEW
Stefano Barelli, Lorenzo Alberio
Massive hemorrhage is a leading cause of death worldwide. During the last decade several retrospective and some prospective clinical studies have suggested a beneficial effect of early plasma-based resuscitation on survival in trauma patients. The underlying mechanisms are unknown but appear to involve the ability of plasma to preserve the endothelial glycocalyx. In this mini-review, we summarize current knowledge on glycocalyx structure and function, and present data describing the impact of hemorrhagic shock and resuscitation fluids on glycocalyx...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29719495/comparative-analysis-of-human-sperm-glycocalyx-from-different-freezability-ejaculates-by-lectin-microarray-and-identification-of-aba-as-sperm-freezability-biomarker
#11
Ai-Jie Xin, Yan-Cheng Wu, Hui Lu, Li Cheng, Yi-Hua Gu, Hua Diao, Guo-Wu Chen, Bin Wu, Zheng Li, Sheng-Ce Tao, Xiao-Xi Sun, Hui-Juan Shi
Background: Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on sperm quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance. Methods: The glycocalyx of good freezability ejaculates was compared with poor freezability ejaculates by lectin microarray. The significant different lectins were validated by flow cytometry (FACS). To analyze the relationship between the potential biomarker and the tolerance of sperm to cryopreservation, 60 samples with different recovery rates were collected and detected the lectin-binding intensity by FACS...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/29717213/metabolic-systems-analysis-of-lps-induced-endothelial-dysfunction-applied-to-sepsis-patient-stratification
#12
Sarah McGarrity, Ósk Anuforo, Haraldur Halldórsson, Andreas Bergmann, Skarphéðinn Halldórsson, Sirus Palsson, Hanne H Henriksen, Pär Ingemar Johansson, Óttar Rolfsson
Endothelial dysfunction contributes to sepsis outcome. Metabolic phenotypes associated with endothelial dysfunction are not well characterised in part due to difficulties in assessing endothelial metabolism in situ. Here, we describe the construction of iEC2812, a genome scale metabolic reconstruction of endothelial cells and its application to describe metabolic changes that occur following endothelial dysfunction. Metabolic gene expression analysis of three endothelial subtypes using iEC2812 suggested their similar metabolism in culture...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29716625/6-hydroxyethyl-starch-hes-130-0-4-diminishes-glycocalyx-degradation-and-decreases-vascular-permeability-during-systemic-and-pulmonary-inflammation-in-mice
#13
Andreas Margraf, Jan M Herter, Katharina Kühne, Anika Stadtmann, Thomas Ermert, Manuel Wenk, Melanie Meersch, Hugo Van Aken, Alexander Zarbock, Jan Rossaint
BACKGROUND: Increased vascular permeability is a pathophysiological hallmark of sepsis and results in increased transcapillary leakage of plasma fluid, hypovolemia, and interstitial edema formation. 6% hydroxyethyl starch (HES 130/0.4) is commonly used to treat hypovolemia to maintain adequate organ perfusion and oxygen delivery. The present study was designed to investigate the effects of 6% HES 130/0.4 on glycocalyx integrity and vascular permeability in lipopolysaccharide (LPS)-induced pulmonary inflammation and systemic inflammation in mice...
May 1, 2018: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29712744/vascular-cell-glycocalyx-mediated-vascular-remodeling-induced-by-hemodynamic-environmental-alteration
#14
Jiajia Liu, Hongyan Kang, Xuejiao Ma, Anqiang Sun, Huiqin Luan, Xiaoyan Deng, Yubo Fan
Vascular remodeling induced by hemodynamic stimuli contributes to the pathophysiology of cardiovascular diseases. The importance of vascular cells (endothelial cells and smooth muscle cells) glycocalyx in the mechanotransduction of flow-induced shear stress at the cellular and molecular levels has been demonstrated over the past decade. However, its potential mechanotransduction role in vascular remodeling has triggered little attention. In the present study, a home-made apparatus was used to expose the rat abdominal aorta to sterile, flow or no flow, normal-pressure or high-pressure conditions for 4 days...
April 30, 2018: Hypertension
https://www.readbyqxmd.com/read/29707296/sevoflurane-did-not-show-better-protective-effect-on-endothelial-glycocalyx-layer-compared-to-propofol-during-lung-resection-surgery-with-one-lung-ventilation
#15
Hye-Jin Kim, Eunsoo Kim, Seung-Hoon Baek, Hee Young Kim, Joo-Yun Kim, Juyeon Park, Eun-Ji Choi
Background: The endothelial glycocalyx layer (EGL) coats the alveolar capillary endothelium and plays important roles in pulmonary vascular protection, modulation, and hemostasis. Ischemia-reperfusion, which occurs during lung resection surgery with one lung ventilation (OLV), can damage the EGL. Sevoflurane is known for its protective effect against ischemia-reperfusion injury. Therefore, we hypothesized that lung resection surgery produces EGL damage and sevoflurane protects the EGL better than the intravenous anesthetic propofol...
March 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29702687/macrophage-migration-inhibitory-factor-is-critical-for-dengue-ns1-induced-endothelial-glycocalyx-degradation-and-hyperpermeability
#16
Hong-Ru Chen, Chiao-Hsuan Chao, Ching-Chuan Liu, Tzong-Shiann Ho, Huey-Pin Tsai, Guey-Chuen Perng, Yee-Shin Lin, Jen-Ren Wang, Trai-Ming Yeh
Vascular leakage is one of the salient characteristics of severe dengue. Nonstructural protein 1 (NS1) of dengue virus (DENV) can stimulate endothelial cells to secrete endothelial hyperpermeability factor, macrophage migration inhibitory factor (MIF), and the glycocalyx degradation factor heparanase 1 (HPA-1). However, it is unclear whether MIF is directly involved in NS1-induced glycocalyx degradation. In this study, we observed that among NS1, MIF and glycocalyx degradation-related molecules, the HPA-1, metalloproteinase 9 (MMP-9) and syndecan 1 (CD138) serum levels were all increased in dengue patients, and only NS1 and MIF showed a positive correlation with the CD138 level in severe patients...
April 27, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29694231/shedding-first-light-on-the-alveolar-epithelial-glycocalyx
#17
Sarah Weidenfeld, Wolfgang M Kuebler
No abstract text is available yet for this article.
April 25, 2018: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/29689364/reversible-renal-glomerular-dysfunction-in-guinea-pigs-exposed-to-glutaraldehyde-polymerized-cell-free-hemoglobin
#18
Xiaoyuan Zhang, Matthew C Williams, Otgonchimeg Rentsendorj, Felice D'Agnillo
Chemically modified hemoglobin (Hb)-based oxygen carriers are promising oxygen replacement therapeutics however their potential renal effects are not fully understood. Using a guinea pig exchange transfusion model, we examined the effects of glutaraldehyde-polymerized bovine hemoglobin (HbG) on the permeability and integrity of the glomerular filtration barrier (GFB), which is comprised of podocytes, fenestrated endothelium, and the glomerular basement membrane. HbG induced marked proteinuria characterized in part by the loss of high molecular weight proteins, including albumin, immunoglobulin, and transferrin, at 4 and 12 h post-infusion that resolved by 72 h...
April 22, 2018: Toxicology
https://www.readbyqxmd.com/read/29679512/phylogenetic-analysis-of-dunaliella-chlorophyta-emphasizing-new-benthic-and-supralittoral-isolates-from-great-salt-lake
#19
William J Henley, Michael Cobbs, Lucie Novoveská, Mark A Buchheim
Dunaliella, a commercially important chlorophyte, is globally distributed in saline habitats. Morphological species have not been definitively reconciled with phylogenetic analyses. Considerable genetic diversity continues to be discovered in new isolates, especially from soil and benthic habitats. Twenty-nine new isolates from Great Salt Lake, Utah, many from benthic or supralittoral habitats, were phylogenetically analyzed using ITS1+5.8S+ITS2 in comparison to a broad sampling of available sequences. A few new isolates align in one branch of a bifurcated monophyletic D...
April 21, 2018: Journal of Phycology
https://www.readbyqxmd.com/read/29658328/effects-of-enoxaparin-on-intravascular-sclerostin-release-in-healthy-men
#20
Jacek Borawski, Justyna Zoltko, Barbara Labij-Reduta, Ewa Koc-Zorawska, Beata Naumnik
Sclerostin (Scl) is implicated in vascular calcification and angiogenesis and localizes within vasculature. Its molecule incorporates a heparin-binding site that implies also binding to endothelial glycocalyx. We preliminary tested whether intravenous (IV) low-molecular-weight heparin enoxaparin can stimulate intravascular release of this calcification inhibitor in humans. Sixteen male volunteers were injected with a bolus of 1 mg/kg body weight of enoxaparin. After 10 minutes, plasma immunoreactive Scl levels increased uniformly by a mean of 184% versus baseline level of 0...
January 1, 2018: Journal of Cardiovascular Pharmacology and Therapeutics
keyword
keyword
12677
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"