Read by QxMD icon Read


Daisuke Muramatsu, Hiroshi Kimura, Kaoru Kotoshiba, Makoto Tachibana, Yoichi Shinkai
Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts directly with HP1. However, the importance of HP1 interaction for Suv39h-mediated H3K9me3 formation at the pericentromere is not well characterized. To address this question, we introduced HP1 binding-defective, N-terminally truncated mouse Suv39h1 (ΔN) into Suv39h-deficient cells...
October 12, 2016: Cell Structure and Function
Xiujun Zhang, Yinbo Qu, Yuqi Qin
BACKGROUND: Heterochromatin protein 1 (HP1, homologue HepA in Penicillium oxalicum) binding is associated with a highly compact chromatin state accompanied by gene silencing or repression. HP1 loss leads to the derepression of gene expression. We investigated HepA roles in regulating cellulolytic enzyme gene expression, as an increasingly number of studies have suggested that cellulolytic enzyme gene expression is not only regulated by transcription factors, but is also affected by the chromatin status...
2016: Biotechnology for Biofuels
Nawras Ghanem, Bärbel Kiesel, Rene Kallies, Hauke Harms, Antonis Chatzinotas, Lukas Y Wick
Although several studies examined the transport of viruses in the terrestrial systems only few studies exist on marine phages (i.e. non-terrestrial viruses infecting marine host bacteria) as sensitively detectable microbial tracers for subsurface colloid transport and water flow. Here, we systematically quantified and compared for the first time the effects of size, morphology and physico-chemical surface properties of six marine phages and two coliphages (MS2, T4) during transport in sand-filled percolated columns...
October 7, 2016: Environmental Science & Technology
Shinji Honda, Vincent T Bicocca, Jordan D Gessaman, Michael R Rountree, Ayumi Yokoyama, Eun Y Yu, Jeanne M L Selker, Eric U Selker
DNA methylation, heterochromatin protein 1 (HP1), histone H3 lysine 9 (H3K9) methylation, histone deacetylation, and highly repeated sequences are prototypical heterochromatic features, but their interrelationships are not fully understood. Prior work showed that H3K9 methylation directs DNA methylation and histone deacetylation via HP1 in Neurospora crassa and that the histone deacetylase complex HCHC is required for proper DNA methylation. The complex consists of the chromodomain proteins HP1 and chromodomain protein 2 (CDP-2), the histone deacetylase HDA-1, and the AT-hook motif protein CDP-2/HDA-1-associated protein (CHAP)...
October 11, 2016: Proceedings of the National Academy of Sciences of the United States of America
Henrique Bridi, Aline Beckenkamp, Gari Vidal Ccana-Ccapatinta, Sérgio Augusto de Loreto Bordignon, Andréia Buffon, Gilsane Lino von Poser
In this study, a phytochemical and biological investigation on five South Brazilian Hypericum species (Hypericum caprifoliatum, Hypericum carinatum, Hypericum connatum, Hypericum myrianthum, and Hypericum polyanthemum) was carried out. The phloroglucinol-enriched fractions (PEF) of the flowering aerial parts were analyzed by high-performance liquid chromatography for the content of uliginosin A (1), japonicin A (2), uliginosin B (3), hyperbrasilol B (4), and the three benzopyrans, that is, 6-isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran (HP1) (5), 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran (HP2) (6), and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) (7)...
September 13, 2016: Phytotherapy Research: PTR
Xing-Yong Liu, Xian-Bo Zhang, Ming-Hui Li, Shu-Qing Zheng, Zhi-Long Liu, Yun-Ying Cheng, De-Shou Wang
Chromobox (Cbx) family proteins are transcriptional repressors that involved in epigenetic and developmental processes. In this study, comprehensive analyses of Cbxs were performed using available genome databases from representative animal species. The Cbx family were originated from one Polycomb (Pc) gene like the yeast Pc, which duplicated into two and gave rise to the Pc and the Heterochromatin protein 1 (Hp1) identified in invertebrates from protozoon to lancelet. Rapid expansion of Cbx family members was observed in vertebrates as ~8 (5 Pc and 3 Hp1) were identified in spotted gar, coelacanth and tetrapods...
September 7, 2016: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Xiaoli Zhang, Xinqiang Liu, Yanli Zhao, Jiasen Cheng, Jiatao Xie, Yanping Fu, Daohong Jiang, Tao Chen
Histone methylation is widely present in animals, plants and fungi, and the methylation modification of histone H3 has important biological functions. Methylation of Lys9 of histone H3 (H3K9) has been proven to regulate chromatin structure, gene silencing, transcriptional activation, plant metabolism, and other processes. In this work, we investigated the functions of a H3K9 methyltransferase gene BcDIM5 in Botrytis cinerea, which contains a PreSET domain, a SET domain and a PostSET domain. Characterization of BcDIM5 knockout transformants showed that the hyphal growth rate and production of conidiophores and sclerotia were significantly reduced, while complementary transformation of BcDIM5 could restore the phenotypes to the levels of wild type...
2016: Frontiers in Microbiology
Katerina Yale, Alan J Tackett, Monica Neuman, Emily Bulley, Brian T Chait, Emily Wiley
The evolutionarily conserved proteins related to heterochromatin protein 1 (HP1), originally described in Drosophila, are well known for their roles in heterochromatin assembly and gene silencing. Targeting of HP1 proteins to specific chromatin locales is mediated, at least in part, by the HP1 chromodomain, which binds to histone H3 methylated at lysine 9 that marks condensed regions of the genome. Mechanisms that regulate HP1 targeting are emerging from studies with yeast and metazoans and point to roles for posttranslational modifications...
July 2016: MSphere
Taylor J R Penke, Daniel J McKay, Brian D Strahl, A Gregory Matera, Robert J Duronio
A defining feature of heterochromatin is methylation of Lys9 of histone H3 (H3K9me), a binding site for heterochromatin protein 1 (HP1). Although H3K9 methyltransferases and HP1 are necessary for proper heterochromatin structure, the specific contribution of H3K9 to heterochromatin function and animal development is unknown. Using our recently developed platform to engineer histone genes in Drosophila, we generated H3K9R mutant flies, separating the functions of H3K9 and nonhistone substrates of H3K9 methyltransferases...
August 15, 2016: Genes & Development
Ruggiero Caizzi, Roberta Moschetti, Lucia Piacentini, Laura Fanti, Renè Massimiliano Marsano, Patrizio Dimitri
The term heterochromatin has been long considered synonymous with gene silencing, but it is now clear that the presence of transcribed genes embedded in pericentromeric heterochromatin is a conserved feature in the evolution of eukaryotic genomes. Several studies have addressed the epigenetic changes that enable the expression of genes in pericentric heterochromatin, yet little is known about the evolutionary processes through which this has occurred. By combining genome annotation analysis and high-resolution cytology, we have identified and mapped 53 orthologs of D...
August 2016: PLoS Genetics
Hua Xie, Qin Wang, Yaqin Chai, Yali Yuan, Ruo Yuan
In this work, a label-free electrochemical biosensor was developed for sensitive and selective detection of mercury (II) ions (Hg(2+)) based on in-situ deposition of silver nanoparticles (AgNPs) on terminal deoxynucleotidyl transferase (TdT) extended ssDNA for signal output and nicking endonuclease for cycling amplification. In the presence of target Hg(2+), the T-rich DNA (HP1) could partly fold into duplex-like structure (termed as output DNA) via T-Hg(2+)-T base pairs and thus exposed its sticky end. The sticky end of output DNA could then hybridize with 3'-PO4 terminated capture DNA (HP2) on electrode surface to form output DNA-HP2 hybridization complex with the sequence 5'-CCTCAGC-3'/3'-GGAGTCG-5' (the sequence could be recognized by nicking endonuclease Nt...
December 15, 2016: Biosensors & Bioelectronics
Kensuke Kataoka, Tomoko Noto, Kazufumi Mochizuki
Multiple heterochromatic loci are often clustered into a higher order nuclear architecture called a heterochromatin body in diverse eukaryotes. Although phosphorylation of Heterochromatin Protein 1 (HP1) family proteins regulates heterochromatin dynamics, its role in heterochromatin bodies remains unknown. We previously reported that dephosphorylation of the HP1-like protein Pdd1p is required for the formation of heterochromatin bodies during the process of programmed DNA elimination in the ciliated protozoan Tetrahymena Here, we show that the heterochromatin body component Jub4p is required for Pdd1p phosphorylation, heterochromatin body formation, and DNA elimination...
August 9, 2016: Proceedings of the National Academy of Sciences of the United States of America
Yajun Wang, Qing Dong, Zhaolan Ding, Kexin Gai, Xiaoyun Han, Farah Naz Kaleri, Qun He, Ying Wang
Catalase-3 (CAT-3) constitutes the main catalase activity in growing hyphae of Neurospora crassa, and its activity increases during exponential growth or is induced under different stress conditions. Although extensive progress has been made to identify catalase regulators, the regulation mechanism of CAT-3 at the chromatin level still remains unclear. Here, we aim at investigating the molecular regulation mechanisms of cat-3 at the chromatin level. We found that CAT-3 protein levels increased in mutants defective in proper global heterochromatin formation...
July 22, 2016: Free Radical Biology & Medicine
Sandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario de Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Favre, Aurélien Olichon, Franck Perez
In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1)...
2016: ELife
Christèle Maison, Delphine Bailly, Jean-Pierre Quivy, Geneviève Almouzni
The trimethylation of histone H3 on lysine 9 (H3K9me3) - a mark recognized by HP1 that depends on the Suv39h lysine methyltransferases (KMTs) - has provided a basis for the reader/writer model to explain HP1 accumulation at pericentric heterochromatin in mammals. Here, we identify the Suv39h1 paralog, as a unique enhancer of HP1α sumoylation both in vitro and in vivo. The region responsible for promoting HP1α sumoylation (aa1-167) is distinct from the KMT catalytic domain and mediates binding to Ubc9. Tethering the 1-167 domain of Suv39h1 to pericentric heterochromatin, but not mutants unable to bind Ubc9, accelerates the de novo targeting of HP1α to these domains...
2016: Nature Communications
Nermina Buljubasic, Rohit M Oemrawsingh, Mirjam B Smeets, Jin M Cheng, Evelyn Regar, Robert-Jan M van Geuns, Patrick W J C Serruys, Eric Boersma, K Martijn Akkerhuis, Isabella Kardys, Fatih Arslan
BACKGROUND: Conflicting results exist regarding the association between a common Haptoglobin (Hp) polymorphism and risk of coronary artery disease. We investigated the association of three functionally different anti-oxidant and anti-inflammatory Hp phenotypes (Hp1-1, Hp2-1, Hp2-2) with invasively measured degree and composition of coronary atherosclerosis as determined by intravascular ultrasound (-virtual histology) (IVUS(-VH)) as well as near-infrared spectroscopy (NIRS). METHODS: Non-culprit coronary artery segments of 581 patients with acute coronary syndrome (ACS) or stable angina pectoris were imaged with IVUS(-VH)...
October 15, 2016: International Journal of Cardiology
Wenwen Wu, Yukiko Togashi, Yoshikazu Johmura, Yasuo Miyoshi, Sachihiko Nobuoka, Makoto Nakanishi, Tomohiko Ohta
The breast and ovarian cancer predisposition protein BRCA1 forms three mutually exclusive complexes with FANCJ (also called BACH1 or BRIP1), CtIP and Abraxas/RAP80 through its BRCT domains, while its RING domain binds to BARD1. We recently found that the interaction of HP1 with BARD1 is required for the accumulation of BRCA1 and CtIP at sites of DNA double-strand breaks (DSBs). Here, we investigated the importance of the HP1 and BARD1-HP1 interaction in the localization of FANCJ together with the other BRCA1-BRCT-binding proteins to clarify the separate role of the HP1-mediated pathway from the RNF8/RNF168-induced ubiquitin-mediated pathway for BRCA1 function...
July 11, 2016: Cancer Science
(no author information available yet)
No abstract text is available yet for this article.
July 1, 2016: Journal of Biological Chemistry
Fan Yang, Yang Wang, Yan He, Haobo Jiang
Extracellular serine protease cascades mediate immune signaling and responses in insects. In the tobacco hornworm Manduca sexta, nearly 30 serine proteases (SPs) and their homologs (SPHs) are cloned from hemocytes and fat body. Some of them participate in prophenoloxidase (proPO) activation and proSpätzle processing. Here we report the cDNA cloning of hemolymph protease-1b (HP1b), which is 90% identical and 95% similar to HP1a (formerly HP1). The HP1a and HP1b mRNA levels in hemocytes was down- and up-regulated after an immune challenge, respectively...
September 2016: Insect Biochemistry and Molecular Biology
Yanhong Zhao, Ning Cheng, Min Dai, Hongquan Pu, Tongzhang Zheng, Haiyan Li, Jie He, Yana Bai
Objective To investigatethe dynamic variation in H3K4me3 and HP1 with employment length in nickel smelting workers. Methods Blood samples were collected from 140 nickel smelting workers and 140 age-matched office workers to test for H3K4me3, and HP1 levels. Results H3K4me3 was statistically significantly different (p < 0.05) between the two groups and positively correlated with employment length (rs=0.267).HP1 was not correlated with employment length (p = 0.066) but was significantly different between the two groups...
June 21, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"