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https://www.readbyqxmd.com/read/29677660/variation-in-bacterial-community-structure-of-aerobic-granular-and-suspended-activated-sludge-in-the-presence-of-the-antibiotic-sulfamethoxazole
#1
Abbass Jafari Kang, Alistair K Brown, Charles S Wong, Zhiyong Huang, Qiuyan Yuan
The treatment performance and bacterial community structure of conventional activated sludge and aerobic granules exposed to antibiotic sulfamethoxazole (SMX) was studied. For three months, two sets of sequencing batch reactors inoculated with conventional and granular biomass were fed with a synthetic municipal wastewater containing 2 μg/L SMX. The presence of SMX had no significant impacts on treatment performance of the reactors as well as stability of the granules. Results confirmed different bacterial community structure of flocs and granules...
April 13, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29675924/new-sts-in-multidrug-resistant-acinetobacter-baumannii-harboring-%C3%AE-lactamases-encoding-genes-isolated-from-brazilian-soils
#2
João Pedro Rueda Furlan, André Pitondo-Silva, Eliana Guedes Stehling
AIMS: We investigated the resistance profile, presence of β-lactamases encoding genes and the clonal relationships in Acinetobacter baumannii isolated from Brazilian soils. METHODS AND RESULTS: Soil isolates of Acinetobacter baumannii were subjected to antimicrobial susceptibility testing by disc diffusion and minimum inhibitory concentration methods. Different β-lactamases encoding genes were screened by PCR and the molecular typing of these isolates was performed through the multilocus sequence typing...
April 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29674848/biofilm-infections-between-scylla-and-charybdis-interplay-of-host-antimicrobial-peptides-and-antibiotics
#3
Sergey Chernysh, Natalia Gordya, Dmitry Tulin, Andrey Yakovlev
Purpose: The aim of this study is to improve the anti-biofilm activity of antibiotics. We hypothesized that the antimicrobial peptide (AMP) complex of the host's immune system can be used for this purpose and examined the assumption on model biofilms. Methods: FLIP7, the AMP complex of the blowfly Calliphora vicina containing a combination of defensins, cecropins, diptericins and proline-rich peptides was isolated from the hemolymph of bacteria-challenged maggots...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29674542/draft-genome-sequences-of-six-multidrug-resistant-clinical-strains-of-acinetobacter-baumannii-isolated-at-two-major-hospitals-in-kuwait
#4
Kother Nasser, Abu Salim Mustafa, Mohd Wasif Khan, Prashant Purohit, Inaam Al-Obaid, Rita Dhar, Wadha Al-Fouzan
Acinetobacter baumannii is an important opportunistic pathogen in global health care settings. Its dissemination and multidrug resistance pose an issue with treatment and outbreak control. Here, we present draft genome assemblies of six multidrug-resistant clinical strains of A. baumannii isolated from patients admitted to one of two major hospitals in Kuwait.
April 19, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29673984/bioinformatics-analysis-and-epitope-screening-of-a-potential-vaccine-antigen-tolb-from-acinetobacter-baumannii-outer-membrane-protein
#5
Xiaojie Song, Hua Zhang, Dongsheng Zhang, Weifeng Xie, Guanghui Zhao
The clinical isolation rate of multidrug-resistant or pan-resistant Acinetobacter baumannii (A. baumannii) is increasing, resulting that optional antibiotics are very limited in clinical practice. To deal with such a dilemma in treatment, the development of effective vaccines serves as a good strategy. Outer membrane proteins (Omp) often contain potential excellent vaccine antigens, and NCBI has published >300 Omp sequences of A. baumannii (including the duplicates). To accurately screen out the potential excellent antigen molecules from a large number of sequences, and avoid repetitive experimental processes is of great significance...
April 16, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29672134/metabolism-and-biodegradation-of-spacecraft-cleaning-reagents-by-strains-of-spacecraft-associated-acinetobacter
#6
Rakesh Mogul, Gregory A Barding, Sidharth Lalla, Sooji Lee, Steve Madrid, Ryan Baki, Mahjabeen Ahmed, Hania Brasali, Ivonne Cepeda, Trevor Gornick, Shawn Gunadi, Nicole Hearn, Chirag Jain, Eun Jin Kim, Thi Nguyen, Vinh Bao Nguyen, Alex Oei, Nicole Perkins, Joseph Rodriguez, Veronica Rodriguez, Gautam Savla, Megan Schmitz, Nicholas Tedjakesuma, Jillian Walker
Spacecraft assembly facilities are oligotrophic and low-humidity environments, which are routinely cleaned using alcohol wipes for benchtops and spacecraft materials, and alkaline detergents for floors. Despite these cleaning protocols, spacecraft assembly facilities possess a persistent, diverse, dynamic, and low abundant core microbiome, where the Acinetobacter are among the dominant members of the community. In this report, we show that several spacecraft-associated Acinetobacter metabolize or biodegrade the spacecraft cleaning reagents of ethanol (ethyl alcohol), 2-propanol (isopropyl alcohol), and Kleenol 30 (floor detergent) under ultraminimal conditions...
April 19, 2018: Astrobiology
https://www.readbyqxmd.com/read/29671618/novel-naphthalimide-nitroimidazoles-as-multitargeting-antibacterial-agents-against-resistant-acinetobacter-baumannii
#7
Jie Kang, Lavanya Gopala, Vijai Kumar Reddy Tangadanchu, Wei-Wei Gao, Cheng-He Zhou
AIM: The increasing emergence of resistant bacteria imposed an urgent request to discover novel antibacterial agents. This work was to develop naphthalimide nitroimidazoles as potentially antibacterial agents. Results/methodology: Compound 9e showed the strong antibacterial activity (minimal inhibitory concentration = 0.013 μmol/ml) against resistant Acinetobacter baumannii (A. baumannii) with rapid killing effect and no obvious triggering of the development of resistance. Its combination use with chloromycin, norfloxacin or clinafloxacin improved the antibacterial potency...
April 19, 2018: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/29670582/boosting-alfalfa-medicago-sativa-l-production-with-rhizobacteria-from-various-plants-in-saudi-arabia
#8
Ihsanullah Daur, Maged M Saad, Abdul Aziz Eida, Shakeel Ahmad, Zahid Hussain Shah, Muhammad Z Ihsan, Yasir Muhammad, Sayed S Sohrab, Heribert Hirt
This study focused on rhizobacteria to promote sustainable crop production in arid regions of Saudi Arabia. The study isolated 17 tightly root-adhering rhizobacteria from various plants at Hada Al Sham in Saudi Arabia. All 17 rhizobacterial isolates were confirmed as plant growth promoting rhizobacteria by classical biochemical tests. Using 16S rDNA gene sequence analyses, the strains were identified as Bacillus, Acinetobacter and Enterobacter . Subsequently, the strains were assessed for their ability to improve the physiology, nutrient uptake, growth, and yield of alfalfa plants grown under desert agriculture conditions...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29669956/nosocomial-pneumonia-search-for-an-empiric-and-effective-antibiotic-regimen-in-high-burden-tertiary-care-centre
#9
Nitin Gupta, Manish Soneja, Yogiraj Ray, Monalisa Sahu, Kutty Sharada Vinod, Arti Kapil, Ashutosh Biswas, Naveet Wig, Rita Sood
The clinical practice guidelines on nosocomial pneumonia recommends an empirical regimen that would work in 95% of the patients based on the local antibiogram. The aim of the study was development of an antibiogram for guiding empiric therapy in settings with high prevalence of multi-drug resistant organisms. A retrospective review of electronic health records (e-hospital portal) was done to analyze all respiratory isolates from patients admitted in medical wards and intensive care unit between May 2016 and May 2017...
April 17, 2018: Drug Discoveries & Therapeutics
https://www.readbyqxmd.com/read/29668902/distinct-amino-acid-residues-confer-one-of-three-udp-sugar-substrate-specificities-in-acinetobacter-baumannii-pglc-phosphoglycosyltransferases
#10
Christian M Harding, M Florencia Haurat, Evgeny Vinogradov, Mario F Feldman
Acinetobacter baumannii is an opportunistic human pathogen with the highest reported rates of multidrug resistance among Gram-negative pathogens. The capsular polysaccharide of A. baumannii is considered one of its most significant virulence factors providing resistance against complemented-mediated killing. Capsule synthesis in A. baumannii is usually initiated by the phosphoglycosyltransferase PglC. PglC transfers a phosphosugar from a nucleotide diphosphate-sugar to a polyprenol phosphate generating a polyprenol diphosphate-linked monosaccharide...
April 13, 2018: Glycobiology
https://www.readbyqxmd.com/read/29668623/differential-expressions-of-biomarkers-in-gingival-crevicular-fluid-of-han-and-uygur-populations-with-peri-implantitis
#11
Xiaowei Gao, Jing Zhou, Yue Sun, Lin Wang, Yanmin Zhou
This study aims to investigate and compare the biomarkers in the gingival crevicular fluid between the Han and Uygur subjects with healthy implants and peri-implantitis.Totally 80 subjects were divided into the H-case (Han patients with peri-implantitis), U-case (Uygur patients with peri-implantitis), H-control (Han subjects with healthy implants), and U-control (Uygur subjects with healthy implants) groups. Cytokine levels in the gingival crevicular fluid were detected, and the dominant bacteria species were analyzed...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29668035/metaproteomics-of-microbiota-in-naturally-fermented-soybean-paste-da-jiang
#12
Ping Zhang, Pengfei Zhang, Mengxi Xie, Feiyu An, Boshu Qiu, Rina Wu
Da-jiang is a typical traditional fermented soybean product in China. At present, the proteins in da-jiang are needed to be explored. The composition and species of microbial proteins in traditional fermented da-jiang were analyzed by metaproteomics based on sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The results showed that the number and variety of microbial proteins in the traditional fermented da-jiang from different regions were different...
April 18, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29667378/alarming-results-of-nosocomial-infections-in-a-multicenter-program-of-surveillance-in-polish-intensive-care-units
#13
Michał Wałaszek, Anna Różańska, Małgorzata Bulanda, Jadwiga Wojkowska-Mach, Polish Society of Hospital Infections Team
INTRODUCTION: Nosocomial bloodstream infections (BSI) among patients in intensive care units (ICU) are the most common form of nosocomial infections and they are serious complications of the treatment process. The etiological factors of these infections and the proper antibiotic therapy can influence the effects of patient treatment. The aim of the study was to analyze the epidemiology, microbiology and antibiotic therapy of nosocomial BSI in Polish ICU patients. MATERIALS AND METHODS: The retrospective study covered patients hospitalized in seven ICUs for adults with a general profile, located in southern Poland...
2018: Przegla̧d Epidemiologiczny
https://www.readbyqxmd.com/read/29666685/identifying-risk-factors-for-healthcare-associated-infections-caused-by-carbapenem-resistant-acinetobacter-baumannii-in-a-neonatal-intensive-care-unit
#14
Amira M Sultan, Wael A Seliem
Objectives: Acinetobacter baumannii is a causative pathogen of various healthcare-associated infections (HAIs) and is particularly prevalent in high-risk hospital settings. This study aimed to determine risk factors associated with HAIs caused by carbapenem-resistant A. baumannii (CRAB) in a neonatal intensive care unit (NICU). Methods: This prospective study was performed between January 2013 and June 2014 among NICU patients at the Mansoura University Children's Hospital, Mansoura, Egypt...
February 2018: Sultan Qaboos University Medical Journal
https://www.readbyqxmd.com/read/29666543/spoilage-of-refrigerated-litopenaeus-vannamei-eavesdropping-on-acinetobacter-acyl-homoserine-lactones-promotes-the-spoilage-potential-of-shewanella-baltica
#15
Suqin Zhu, Haohao Wu, Caili Zhang, Jinxin Jie, Zunying Liu, Mingyong Zeng, Changyun Wang
Shewanella baltica and Acinetobacter are among the predominant spoilage bacteria in refrigerated shrimp ( Litopenaeus vannamei ). S. baltica are incapable of producing acyl-homoserine lactone (AHL) quorum sensing signals, but can respond to environmental AHLs. In this paper, Acinetobacter was found to produce three AHLs, i.e. N -butanoyl-l-homoserine lactone (C4-HSL), N -(3-oxohexanoyl)-l-homoserine lactone (O-C6-HSL) and N -(3-oxooctanoyl)-l-homoserine lactone (O-C8-HSL), according to thin-layer chromatography using the bioreporter Agrobacterium tumefaciens A136...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665446/absence-of-tetb-identifies-minocycline-susceptible-isolates-of-acinetobacter-baumannii
#16
Olga Lomovskaya, Dongxu Sun, Debora Rubio-Aparicio, Kirk J Nelson, Visanu Thamlikitkul, Michael N Dudley, Mark A Redell
BACKGROUND: Minocycline is one of the few options available to treat infections caused by Acinetobacter baumannii. Acquired resistance is associated with the presence of the TetB efflux pump. Previous studies suggest that the absence of tetB may predict minocycline MICs of ≤4 mg/L. METHODS: A collection of 258 isolates of A. baumannii was used to generate MIC frequency distributions for the tetB positive and negative sets of isolates. RESULTS: Of the tetB-negative strains, all 93 had minocycline MICs ≤4 mg/L, resulting in a negative predictive value of 100%...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29665444/ndm-1-and-oxa-23-producing-acinetobacter-baumannii-isolated-from-intensive-care-unit-patients-in-tunisia
#17
Elaa Maamar, Carla Andrea Alonso, Sana Ferjani, Ali Jendoubi, Zaineb Hamzaoui, Alia Jebri, Mabrouka Saidani, Salma Ghedira, Carmen Torres, Ilhem Boutiba-Ben Boubaker
Gastrointestinal colonization by carbapenem-resistant Acinetobacter baumannii (CRAB), is a critical step before nosocomial infection. Our objective was to evaluate CRAB intestinal carriage in patients admitted in the intensive care unit (ICU) of a Tunisian Hospital and to determine the antimicrobial resistance mechanisms involved. From December-2014 to February-2015, all 63 patients admitted at the ICU were screened for rectal CRAB-colonization upon admission and once weekly thereafter. ICU-patients who acquired a CRAB-nosocomial-infection were also included...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29665443/activity-of-a-nitric-oxide-generating-wound-treatment-system-against-wound-pathogen-biofilms
#18
Richard D Waite, Joanne E Stewart, Abish S Stephen, Robert P Allaker
Wound bioburden plays an important role in impaired healing and the development of infection-related complications. The objective of this study was to determine the efficacy of an innovative two-layer nitric oxide generating system (NOx) to prevent and treat biofilms formed by bacterial and fungal pathogens commonly associated with wound infection, and activity against Pseudomonas aeruginosa virulence factors. Single and mixed species biofilms were grown for 24h on nitrocellulose filters placed on agar. Filters were covered with either NOx or placebo, before and after biofilm formation...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29664968/water-system-is-a-controlling-variable-modulating-bacterial-diversity-of-gastrointestinal-tract-and-performance-in-rainbow-trout
#19
Omolola C Betiku, Carl J Yeoman, T Gibson Gaylord, Benjamin Americus, Sarah Olivo, Glenn C Duff, Wendy M Sealey
A two-phase feeding study evaluating performance of rainbow trout and comparing luminal and mucosal gastrointestinal tract (GIT) bacterial community compositions when fed two alternative protein diets in two rearing systems was conducted. Alternative protein diets (animal protein and plant protein diets) balanced with crystalline amino acids: lysine, methionine and threonine or unbalanced, were fed to rainbow trout in two separate water systems (recirculating (RR) and flow-through (FF)) for a period of 16 weeks...
2018: PloS One
https://www.readbyqxmd.com/read/29664950/body-lice-of-homeless-people-reveal-the-presence-of-several-emerging-bacterial-pathogens-in-northern-algeria
#20
Meriem Louni, Nassima Mana, Idir Bitam, Mustapha Dahmani, Philippe Parola, Florence Fenollar, Didier Raoult, Oleg Mediannikov
BACKGROUND: Human lice, Pediculus humanus, are obligate blood-sucking parasites. Body lice, Pediculus h. humanus, occur in two divergent mitochondrial clades (A and D) each exhibiting a particular geographic distribution. Currently, the body louse is recognized as the only vector for louse-borne diseases. In this study, we aimed to study the genetic diversity of body lice collected from homeless populations in three localities of northern Algeria, and to investigate louse-borne pathogens in these lice...
April 17, 2018: PLoS Neglected Tropical Diseases
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