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https://www.readbyqxmd.com/read/28631638/clinicomicrobiological-profile-of-endophthalmitis-a-10-year-experience-in-a-tertiary-care-center-in-north-india
#1
Gita Satpathy, Niranjan Nayak, Meenakshi Wadhwani, Pradeep Venkwatesh, Atul Kumar, Yograj Sharma, Vishnu Sreenivas
PURPOSE: To determine the clinicomicrobiological profile of infectious agents and their antibiotic susceptibility in different type of endophthalmitis. METHODS: A retrospective review of clinical and microbiological records from January 2001 to December 2010, was performed in 1110 patients diagnosed with different type of endophthalmitis (postoperative, posttraumatic, endogenous and post keratitis) to record the demographic details, clinical presentations; microbiological agents isolated with their antimicrobial sensitivity pattern...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28631634/phenotypic-and-molecular-characterization-of-cefotaximases-temoniera-and-sulfhydryl-variable-%C3%AE-lactamases-in-pseudomonas-and-acinetobacter-isolates-in-an-indian-tertiary-health-care-center
#2
Sana Jamali, Mohammed Shahid, Farrukh Sobia, Anuradha Singh, Haris M Khan
BACKGROUND: Cefotaximases (CTX-M), temoniera (TEM), and sulfhydryl variable (SHV) constitute a rapidly growing cluster of enzymes that have disseminated geographically. They are spreading to species other than Enterobacteriaceae and might be responsible for the presence of blaCTX-M,blaTEM, and blaSHVgenes in Pseudomonas and Acinetobacter spp. The present study was designed to characterize CTX-M, TEM, and SHV phenotypically and genotypically in Pseudomonas and Acinetobacter spp. MATERIALS AND METHODS: A total of 90 isolates (73 Pseudomonas and 17 Acinetobacter spp...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28630311/a-prokaryotic-viral-sequence-is-expressed-and-conserved-in-mammalian-brain
#3
Yang-Hui Yeh, Vignesh Gunasekharan, Laura Manuelidis
A natural and permanent transfer of prokaryotic viral sequences to mammals has not been reported by others. Circular "SPHINX" DNAs <5 kb were previously isolated from nuclease-protected cytoplasmic particles in rodent neuronal cell lines and brain. Two of these DNAs were sequenced after Φ29 polymerase amplification, and they revealed significant but imperfect homology to segments of commensal Acinetobacter phage viruses. These findings were surprising because the brain is isolated from environmental microorganisms...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28630181/in-vitro-activity-of-the-siderophore-cephalosporin-cefiderocol-against-a-recent-collection-of-clinically-relevant-gram-negative-bacilli-from-north-america-and-europe-including-carbapenem-non-susceptible-isolates-the-sidero-wt-2014-study
#4
Meredith A Hackel, Masakatsu Tsuji, Yoshinori Yamano, Roger Echols, James A Karlowsky, Daniel F Sahm
Cefiderocol (formerly S-649266) is an investigational siderophore cephalosporin. Iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) was prepared according to the Clinical and Laboratory Standards Institute (CLSI) protocol and used to perform broth microdilution testing of cefiderocol against a 2014-2015 collection of clinical isolates of gram-negative bacilli from North America (n=4,239) and Europe (n=4,966). The concentration of cefiderocol inhibiting 90% of isolates tested (MIC90) was: 0.5 (North America; n=3,007) and 1 μg/ml (Europe; n=3,080) for all isolates of Enterobacteriaceae; 1 (North America; n=30) and 4 μg/ml (Europe; n=139) for meropenem-non-susceptible (MIC ≥2 μg/ml) isolates of Enterobacteriaceae; 0...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28630178/efficacy-of-cefiderocol-against-carbapenem-resistant-gram-negative-bacilli-in-immunocompetent-rat-respiratory-tract-infection-models-recreating-human-plasma-pharmacokinetics
#5
Shuhei Matsumoto, Christine M Singley, Jennifer Hoover, Rio Nakamura, Roger Echols, Stephen Rittenhouse, Masakatsu Tsuji, Yoshinori Yamano
Cefiderocol (S-649266), a novel siderophore cephalosporin, shows potent activity against carbapenem-resistant Gram-negative bacilli. In this study, we evaluated the efficacy of cefiderocol against carbapenem-resistant Gram-negative bacilli (Pseudomonas aeruginosa,Acinetobacter baumannii and Klebsiella pneumoniae) in immunocompetent rat respiratory tract infection models recreating plasma pharmacokinetics (PK) profiles in healthy human subjects. A total of 6 clinical isolates (1 cephalosporin-susceptible P. aeruginosa, 1 multidrug-resistant P...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28629972/characterization-of-dioxygenases-and-biosurfactants-produced-by-crude-oil-degrading-soil-bacteria
#6
Santhakumar Muthukamalam, Sivalingam Sivagangavathi, Dharmapal Dhrishya, Sadras Sudha Rani
Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28626737/high-level-bacterial-contamination-of-secondary-school-students-mobile-phones
#7
Siiri Kõljalg, Rando Mändar, Tiina Sõber, Tiiu Rööp, Reet Mändar
INTRODUCTION: While contamination of mobile phones in the hospital has been found to be common in several studies, little information about bacterial abundance on phones used in the community is available. Our aim was to quantitatively determine the bacterial contamination of secondary school students' mobile phones. METHODS: Altogether 27 mobile phones were studied. The contact plate method and microbial identification using MALDI-TOF mass spectrometer were used for culture studies...
June 2017: Germs
https://www.readbyqxmd.com/read/28626312/bacterial-contamination-in-physical-therapy-departments-in-the-state-of-kuwait
#8
Sameera H Aljadi, Mashael Al-Shemmari, Jumana Al-Ramzi, Shoug Al-Abdullatif, Zahraa Hajeyah, Laila Jamal, Sara Al-Bahar
[Purpose] Interferential therapy and electrical stimulation are electrophysical modalities commonly used in physical therapy departments to treat patients with musculoskeletal problems. These machines are applied directly to the patient's skin via a medium or electrodes, which can facilitate the transmission of microorganisms from one patient to another. The purpose of this study was to determine the extent of microorganism contamination in the machines sponges at physical therapy departments in Kuwait hospitals...
June 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28625698/a-cold-hard-menace-a-contaminated-ice-machine-as-a-potential-source-for-transmission-of-carbapenem-resistant-acinetobacter-baumannii
#9
Anubhav Kanwar, T Nicholas Domitrovic, Sreelatha Koganti, Peter Fuldauer, Jennifer L Cadnum, Robert A Bonomo, Curtis J Donskey
During an investigation of potential sources of transmission of multidrug-resistant gram-negative bacilli on a spinal cord injury unit, we recovered genetically related carbapenem-resistant Acinetobacter baumannii isolates from the stool of 3 patients, the hands of a nurse, and an ice machine water outlet spout and drain. Our findings suggest that contaminated ice machines could serve as a potential reservoir for dissemination of multidrug-resistant gram-negative bacilli.
June 15, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28624549/evaluation-of-a-new-trilocus-sequence-based-multiplex-pcr-to-detect-major-acinetobacter-baumannii-clonal-complexes-circulating-in-brazil
#10
Francielli Mahnic de Vasconcellos, Monique Ribeiro Tiba Casas, Laís Calissi Brisolla Tavares, William Vaz de Sousa, Doroti de Oliveira Garcia, Carlos Henrique Camargo
Dissemination of carbapenem-resistant Acinetobacter baumannii (CRAB) is mainly due to the spread of clonal lineages, particularly those included into the clonal complexes (CC) CC1, CC2, CC15, CC25, and CC79. We evaluated the usefulness of a recently modified PCR-based trilocus sequence-based typing (m3LST) in comparison with the standard multilocus sequence typing (MSLT) of 7 housekeeping genes as per the Institute Pasteur Scheme to assign the clonal complexes in CRAB. A collection of 78 CRAB isolated from 67 different Brazilian health institutions was submitted to both methodologies, and concordance rate was calculated...
June 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28623298/constraint-based-modeling-identifies-new-putative-targets-to-fight-colistin-resistant-a-baumannii-infections
#11
Luana Presta, Emanuele Bosi, Leila Mansouri, Lenie Dijkshoorn, Renato Fani, Marco Fondi
Acinetobacter baumannii is a clinical threat to human health, causing major infection outbreaks worldwide. As new drugs against Gram-negative bacteria do not seem to be forthcoming, and due to the microbial capability of acquiring multi-resistance, there is an urgent need for novel therapeutic targets. Here we have derived a list of new potential targets by means of metabolic reconstruction and modelling of A. baumannii ATCC 19606. By integrating constraint-based modelling with gene expression data, we simulated microbial growth in normal and stressful conditions (i...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28622734/epidemiology-of-multidrug-resistant-acinetobacter-baumannii-strains-in-iran-a-systematic-review-and-meta-analysis
#12
Abed Zahedi Bialvaei, Ebrahim Kouhsari, Amin Salehi-Abargouei, Nour Amirmozafari, Rashid Ramazanzadeh, Ali Ghadimi-Daresajini, Mansour Sedighi
Acinetobacter baumannii is an important opportunistic pathogen that causes major public health concern especially in hospitalized patients due to the acquisition of multidrug resistance (MDR). The aim of this study was to systematically review published data about the prevalence rate of MDR-A. baumannii (MDR-AB) from different parts of Iran and provide an overall relative frequency (RF) using meta-analysis. All available national and international databanks were searched to find published studies up to June 2016...
June 16, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/28622477/an-examination-of-factors-affecting-the-length-of-stay-in-a-palliative-care-center
#13
Metin Dincer, Kadriye Kahveci, Cihan Doger
BACKGROUND: With the increase in elderly population, life-threatening chronic diseases are increasing, simultaneously increasing the need for palliative care centers (PCCs). OBJECTIVES: To evaluate the factors affecting the length of stay (LOS) and discharge of patients from a PCC. METHODS: A retrospective scan was made of the records of patients followed up in the PCC between January 2013 and March 2016. A record was made of patient age, gender, diagnosis, conditions/comorbidities, Glasgow Coma Scale, Karnofsky Performance Scale, LOS, prognosis (exitus or surviving), percutaneous endoscopic gastrostomy (PEG), tracheostomy, mechanical ventilator, nutrition (total parenteral nutrition [TPN] or enteral nutrition), and the results of cultures taken during stay in PCCs (blood, tracheal aspirate, urine, rectal swab, wound)...
June 16, 2017: Journal of Palliative Medicine
https://www.readbyqxmd.com/read/28622224/too-much-too-soon-or-too-little-too-late-the-challenge-of-preventing-acinetobacter-transmissions
#14
Brooke K Decker
No abstract text is available yet for this article.
July 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28621711/indigenous-probiotic-lactobacillus-isolates-presenting-antibiotic-like-activity-against-human-pathogenic-bacteria
#15
Debashis Halder, Manisha Mandal, Shiv Sekhar Chatterjee, Nishith Kumar Pal, Shyamapada Mandal
BACKGROUND: Indigenous lactic acid bacteria are well known probiotics having antibacterial activity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. METHODS: Four curd samples were processed microbiologically for the isolation of lactic acid bacteria (LAB). The LAB strains obtained were identified by conventional methods: cultural aspect, gram-staining, biochemical and sugar fermentation tests...
June 16, 2017: Biomedicines
https://www.readbyqxmd.com/read/28621110/characterization-of-microbial-communities-in-chinese-rice-wine-collected-at-yichang-city-and-suzhou-city-in-china
#16
Yucai Lyu, Yanli Gong, Yajie Li, Zejiang Pan, Yi Yao, Ning Li, Jinling Guo, Dachun Gong, Yihong Ti, Caiyun Peng
Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 d. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and 292.50 and 12.31 mg/g, respectively, for rice wine from Suzhou city. Acetic acid and lactic acid were the most abundant organic acids. Abundant fungi and bacteria were detected in both communities by high-throughput sequencing...
June 16, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28620784/inhibitory-effect-of-taurine-on-biofilm-formation-during-alkane-degradation-in-acinetobacter-oleivorans-dr1
#17
Hyo Jung Eom, Woojun Park
Taurine, 2-aminoethanesulfonate, is known to function as an antioxidant or membrane stabilizer in eukaryotic cells, but its role in bacteria has been poorly characterized. Biofilm formation of Acinetobacter oleivorans DR1 was significantly reduced by taurine only during alkane degradation, suggesting that taurine affects alkane-induced cell surface. Structurally similar compounds harboring an amine group such as hypotaurine or ethylenediamine have a similar effect, which was not observed with sulfonate-containing chemicals such as ethanesulfonic acid, hexanesulfonic acid...
June 15, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28619790/complete-genome-sequence-of-acinetobacter-calcoaceticus-ca16-a-bacterium-capable-of-degrading-diesel-and-lignin
#18
Margaret T Ho, Brian Weselowski, Ze-Chun Yuan
We report here the complete assembled genome sequence of Acinetobacter calcoaceticus CA16, which is capable of utilizing diesel and lignin as a sole carbon source. CA16 contains a 4,110,074-bp chromosome and a 5,920-bp plasmid. The assembled sequences will help elucidate potential metabolic pathways and mechanisms responsible for CA16's hydrocarbon degradation ability.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28619105/patterns-of-antimicrobial-resistance-in-intensive-care-unit-patients-a-study-in-vietnam
#19
Giang M Tran, Thao P Ho-Le, Duc T Ha, Chau H Tran-Nguyen, Tuyet S M Nguyen, Thao T N Pham, Tuyet A Nguyen, Dung A Nguyen, Hoa Q Hoang, Ngoc V Tran, Tuan V Nguyen
BACKGROUND: Antimicrobial resistance has emerged as a major concern in developing countries. The present study sought to define the pattern of antimicrobial resistance in ICU patients with ventilator-associated pneumonia. METHODS: Between November 2014 and September 2015, we enrolled 220 patients (average age ~ 71 yr) who were admitted to ICU in a major tertiary hospital in Ho Chi Minh City, Vietnam. Data concerning demographic characteristics and clinical history were collected from each patient...
June 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28618727/analysis-of-epidemiology-and-risk-factors-for-mortality-in-ventilator-associated-pneumonia-attacks-in-intensive-care-unit-patients
#20
Ayşe But, Meltem Arzu Yetkin, Dilek Kanyilmaz, Halide Aslaner, Aliye Baştuğ, Adalet Aypak, Pınar Öngürü, Esragül Akinci, Nevzat Mehmet Mutlu, Hürrem Bodur
BACKGROUND/AIM: The aim of this study was to investigate the epidemiologic characteristics, the causative microorganisms and their antimicrobial susceptibility patterns, and the prognostic risk factors for mortality in critically ill patients with ventilator-associated pneumonia (VAP). MATERIALS AND METHODS: In this retrospective observational study, all the critically ill patients with VAP hospitalized in a medical/surgical intensive care unit (ICU) between January 2010 and June 2015 were evaluated...
June 12, 2017: Turkish Journal of Medical Sciences
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