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https://www.readbyqxmd.com/read/28098900/endothelial-microparticles-activate-endothelial-cells-to-facilitate-the-inflammatory-response
#1
Yiyun Liu, Ruyuan Zhang, Hongping Qu, Jun Wu, Lei Li, Yaoqing Tang
Endothelial microparticles (EMPs) and endothelial cells (ECs) are involved in the pathophysiological mechanisms of sepsis and septic shock. EMPs are small vesicles released by ECs and are considered biomarkers for endothelial cell function and mediators for intercellular information exchange. However, the effect of EMPs on their parental ECs remains unknown. The present study collected tumor necrosis factor‑α‑derived EMPs and detected the proinflammatory cytokines released from unstimulated and EMP‑stimulated ECs by proteome profiler array...
January 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28098891/nhe1-is-upregulated-in-gastric-cancer-and-regulates-gastric-cancer-cell-proliferation-migration-and-invasion
#2
Rui Xie, Haibo Wang, Hai Jin, Guorong Wen, Biguang Tuo, Jingyu Xu
Na+/H+ exchanger isoform 1 (NHE1) is known to play a key role in regulating intracellular pH and osmotic homeostasis and is involved in the development and progression of several types of cancer. However, the function and specific mechanism of NHE1 in gastric cancer (GC) are not clearly understood. In the present study, we report that NHE1 is overexpressed in tissues and cell lines from GC patients, and knockdown or inhibition of NHE1 suppressed GC cell proliferation via regulation of G1/S and G2/M cell cycle phase transitions, concomitant with a marked decrease in positive cell cycle regulators, including cyclin D1 and cyclin B1...
January 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28098750/cluster-based-maximum-consensus-time-synchronization-for-industrial-wireless-sensor-networks
#3
Zhaowei Wang, Peng Zeng, Mingtuo Zhou, Dong Li, Jintao Wang
Time synchronization is one of the key technologies in Industrial Wireless Sensor Networks (IWSNs), and clustering is widely used in WSNs for data fusion and information collection to reduce redundant data and communication overhead. Considering IWSNs' demand for low energy consumption, fast convergence, and robustness, this paper presents a novel Cluster-based Maximum consensus Time Synchronization (CMTS) method. It consists of two parts: intra-cluster time synchronization and inter-cluster time synchronization...
January 13, 2017: Sensors
https://www.readbyqxmd.com/read/28098357/partial-purification-characterization-and-thermal-inactivation-kinetics-of-peroxidase-and-polyphenol-oxidase-isolated-from-kalipatti-sapota-manilkara-zapota
#4
Vishwasrao Chandrahas, Chakraborty Snehasis, Ananthanarayan Laxmi
BACKGROUND: The extraction, purification, and characterization of peroxidase (POD) and polyphenol oxidase (PPO) were studied for Kalipatti sapota fruit. The crude enzyme extract was partially purified by ammonium sulfate precipitation followed by BioGel P100 size exclusion and Unosphere Q anion-exchange chromatography. RESULTS: Molecular weights of 20 kDa (POD) and 24 kDa (PPO) were indicated by SDS-PAGE. A single band was observed on SDS-PAGE with a fold purity of 10...
January 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28098339/the-effect-of-blue-light-on-stomatal-oscillations-and-leaf-turgor-pressure-in-banana-leaves
#5
Yotam Zait, Or Shapira, Amnon Schwartz
Stomatal oscillations are cyclic opening and closing of stomata, presumed to initiate from hydraulic mismatch between leaf water supply and transpiration rate. To test this assumption, mismatches between water supply and transpiration were induced using manipulations of VPD (vapor pressure deficit) and light spectrum in banana (Musa acuminata). Simultaneous measurements of gas exchange with changes in leaf turgor pressure were used to describe the hydraulic mismatches. An increase of VPD above a certain threshold, caused stomatal oscillations with variable amplitudes...
January 18, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28098337/characterisation-of-the-enzymatic-properties-of-mpapr1-an-aspartic-protease-secreted-by-the-wine-yeast-metschnikowia-pulcherrima
#6
Louwrens Wiid Theron, Marina Bely, Benoit Divol
BACKGROUND: MpAPr1, encoding an acid protease from the wine yeast Metschnikowia pulcherrima IWBT Y1123, was previously isolated and shown to display potential activity against casein and grape proteins. However, its characterisation remained partial. RESULTS: MpAPr1 was cloned into the pGAPZαA vector and transformed into Komagataella pastoris X33 for heterologous expression. After verification of activity, the enzyme properties were characterised. Protease activity within the concentrated supernatant was retained over a pH range of 3...
January 18, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28098290/in-situ-ligand-exchange-mediated-0d-1d-transformation-of-a-polyoxovanadate
#7
M Wendt, P Polzin, J van Leusen, C Näther, P Kögerler, W Bensch
The antimonato-polyoxovanadate {Ni(II)(en)3}3[VSbO42(H2O)]·ca.15H2O was utilized as a synthon for the solvothermal in situ generation of the new compound {Ni(II)(phen)3}2[{Ni(II)(en)2}VSbO42(H2O)]·19H2O, a rearrangement induced by ligand metathesis. While in the precursor structure cations and anions are isolated, the solid-state structure of the product is characterized by 1D chains consisting of alternating [V15Sb6O42(H2O)](6-) cluster shells and [Ni(en)2](2+) units covalently linked to neighboring clusters via terminal oxygen atoms...
January 18, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28098267/a-tailored-multi-frequency-epr-approach-to-accurately-determine-the-magnetic-resonance-parameters-of-dynamic-nuclear-polarization-agents-application-to-amupol
#8
P Gast, D Mance, E Zurlo, K L Ivanov, M Baldus, M Huber
To understand the dynamic nuclear polarization (DNP) enhancements of biradical polarizing agents, the magnetic resonance parameters need to be known. We describe a tailored EPR approach to accurately determine electron spin-spin coupling parameters using a combination of standard (9 GHz), high (95 GHz) and ultra-high (275 GHz) frequency EPR. Comparing liquid- and frozen-solution continuous-wave EPR spectra provides accurate anisotropic dipolar interaction D and isotropic exchange interaction J parameters of the DNP biradical AMUPol...
January 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28098142/carbon-translocation-from-a-plant-to-an-insect-pathogenic-endophytic-fungus
#9
Scott W Behie, Camila C Moreira, Irina Sementchoukova, Larissa Barelli, Paul M Zelisko, Michael J Bidochka
Metarhizium robertsii is a common soil fungus that occupies a specialized ecological niche as an endophyte and an insect pathogen. Previously, we showed that the endophytic capability and insect pathogenicity of Metarhizium are coupled to provide an active method of insect-derived nitrogen transfer to a host plant via fungal mycelia. We speculated that in exchange for this insect-derived nitrogen, the plant would provide photosynthate to the fungus. By using (13)CO2, we show the incorporation of (13)C into photosynthate and the subsequent translocation of (13)C into fungal-specific carbohydrates (trehalose and chitin) in the root/endophyte complex...
January 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28098045/-on-the-timing-of-randomised-studies-the-sooner-initiated-the-better
#10
R H H Groenwold, P M M Bossuyt
The study design for randomised double-blind studies is powerful. Randomisation and blinding ensure that the groups that are compared are truly exchangeable. Any differences in health outcomes can be attributed rightfully to the one aspect on which the study groups differ: the treatment. In this commentary, we argue that the use of this powerful study design at the wrong moment can lead to undesirable situations.
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28097910/purification-and-biochemical-characterization-of-a-novel-thermostable-serine-alkaline-protease-from-aeribacillus-pallidus-c10-a-potential-additive-for-detergents
#11
Vildan Yildirim, Mustafa Ozkan Baltaci, Ilknur Ozgencli, Melda Sisecioglu, Ahmet Adiguzel, Gulsah Adiguzel
An extracellular thermostable alkaline serine protease enzyme from Aeribacillus pallidus C10 (GenBank No: KC333049), was purified 4.85 and 17. 32-fold with a yield of 26.9 and 19.56%, respectively, through DE52 anion exchange and Probond affinity chromatography. The molecular mass of the enzyme was determined through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with approximately 38.35 kDa. The enzyme exhibited optimum activity at pH 9 and at temperature 60 °C. It was determined that the enzyme had remained stable at the range of pH 7...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28097753/fully-copper-exchanged-high-silica-lta-zeolites-as-unrivaled-hydrothermally-stable-nh3-scr-catalysts
#12
Taekyung Ryu, Nak Ho Ahn, Seungwan Seo, Jung Cho, Hyojun Kim, Donghui Jo, Gi Tae Park, Pyung Soon Kim, Chang Hwan Kim, Elliott L Bruce, Paul A Wright, In-Sik Nam, Suk Bong Hong
Diesel engine technology is still the most effective solution to meet tighter CO2 regulations in the mobility and transport sector. In implementation of fuel-efficient diesel engines, the poor thermal durability of lean nitrogen oxides (NOx ) aftertreatment systems remains as one major technical hurdle. Divalent copper ions when fully exchanged into high-silica LTA zeolites are demonstrated to exhibit excellent activity maintenance for NOx reduction with NH3 under vehicle simulated conditions even after hydrothermal aging at 900 °C, a critical temperature that the current commercial Cu-SSZ-13 catalyst cannot overcome owing to thermal deactivation...
January 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28097675/the-swamplands-of-reflection-using-conversation-analysis-to-reveal-the-architecture-of-group-reflection-sessions
#13
Mario Veen, Anne de la Croix
CONTEXT: Many medical schools include group reflection in their curriculum, and many researchers have considered both the concept and the outcomes of reflection. However, no research has been carried out on how 'reflective talk' is structured in the classroom. This paper describes how tutors and residents organise group reflection sessions in situ by describing an example of group reflection in medical education. Our aim is to provide an evidence base that can be used by medical educators to think about the way reflection should be included in their curriculum...
January 18, 2017: Medical Education
https://www.readbyqxmd.com/read/28097651/the-mysterious-role-of-the-neuronal-anion-exchanger-3
#14
Eric Delpire
No abstract text is available yet for this article.
January 17, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28097485/sorption-of-organic-phosphates-and-its-effects-on-aggregation-of-hematite-nanoparticles-in-monovalent-and-bivalent-solutions
#15
Chen-Yang Xu, Jiu-Yu Li, Ren-Kou Xu, Zhi-Neng Hong
Sorption of organic phosphates-myo-inositol hexakisphosphate (IHP) and glycerol phosphate (GP) and its effects on the early stage of hematite aggregation kinetics were investigated at different pH and electrolyte composition. KH2PO4 (KP) was taken as an inorganic P source for comparison. Results indicated that for all types of P, the sorption amounts decreased with increasing solution pH. Sorption amount of IHP was almost two times that of KP, while those of GP and KP were close. Both organic P and inorganic P interacted with hematite via ligand exchange through their phosphate groups, which conveyed negative charges to mineral surface and significantly decreased the zeta potential of hematite...
January 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28097168/purification-and-biochemical-properties-of-a-thermostable-haloalkaline-cellulase-from-bacillus-licheniformis-amf-07-and-its-application-for-hydrolysis-of-different-cellulosic-substrates-to-bioethanol-production
#16
Fatemeh Azadian, Arastoo Badoei-Dalfard, Abdolhamid Namaki-Shoushtari, Mehdi Hassanshahian
A thermophilic strain AMF-07, hydrolyzing carboxymethylcellulose (CMC) was isolated from Kerman hot spring and was identified as Bacillus licheniformis based on 16S rRNA sequence homology. The carboxymethylcellulase (CMCase) enzyme produced by the B. licheniformis was purified by (NH4)2SO4 precipitation, ion exchange and gel filtration chromatography. The purified enzyme gave a single band on SDS- PAGE with a molecular weight of 37 kDa. The CMCase enzyme was highly active and stable over broad ranges of temperature (40-80ºC), pH (6...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097167/expression-purification-and-kinetic-characterization-of-recombinant-benzoate-dioxygenase-from-rhodococcus-ruber-ukmp-5m
#17
Arezoo Tavakoli, Ainon Hamzah, Amir Rabu
In this study, benzoate dioxygenase from Rhodococcus ruber UKMP-5M was catalyzed by oxidating the benzene ring to catechol and other derivatives. The benzoate dioxygenase (benA gene) from Rhodococcus ruber UKMP-5M was then expressed, purified, characterized, The benA gene was amplified (642 bp), and the product was cloned into a pGEM-T vector. The recombinant plasmid pGEMT-benA was digested by double restriction enzymes BamHI and HindIII to construct plasmid pET28b-benA and was then ligated into Escherichia coli BL21 (DE3)...
September 2016: Molecular Biology Research Communications
https://www.readbyqxmd.com/read/28097115/granulomatous-lung-disease-a-novel-complication-following-metallosis-from-hip-arthroplasty
#18
Theodoros Balbouzis, Thomas Georgiadis, Peter Grigoris
A case of a female patient with local and systemic complications of metallosis, following catastrophic wear of a revised hip arthroplasty, is presented. The patient had a history of a fractured ceramic-on-ceramic implant, exchanged with a metal-on-polyethylene prosthesis. Systemic complications included sarcoidosis-like reactions, presenting as granulomatous lung disease, along with chorioretinitis, erythema nodosum, and cardiomyopathy. High local and circulating cobalt and chromium levels established the diagnosis...
December 2016: Hip & Pelvis
https://www.readbyqxmd.com/read/28097039/the-concept-of-wind-in-traditional-chinese-medicine
#19
REVIEW
Mehrab Dashtdar, Mohammad Reza Dashtdar, Babak Dashtdar, Karima Kardi, Mohammad Khabaz Shirazi
The use of folk medicine has been widely embraced in many developed countries under the name of traditional, complementary and alternative medicine (TCAM) and is now becoming the mainstream in the UK and the rest of Europe, as well as in North America and Australia. Diversity, easy accessibility, broad continuity, relatively low cost, base levels of technological inputs, fewer side effects, and growing economic importance are some of the positive features of folk medicine. In this framework, a critical need exists to introduce the practice of folk medicine into public healthcare if the goal of reformed access to healthcare facilities is to be achieved...
December 2016: Journal of Pharmacopuncture
https://www.readbyqxmd.com/read/28096988/successful-outcome-of-three-patients-with-sickle-cell-disease-and-fat-embolism-syndrome-treated-with-intensive-exchange-transfusion
#20
Paul Greaves, Vivek Mathew, Catherine Peters, Susan Rowe, Roger J Amos, Dimitris A Tsitsikas
Fat embolism syndrome (FES) is a rare complication of sickle-cell disease (SCD) associated with extremely high mortality rates. It affects predominantly non-SS patients and those with previously mild disease. Rapid institution of exchange transfusion with an aim to reduce HbS to very low levels as soon as FES is suspected can be life-saving.
January 2017: Clinical Case Reports
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